diff --git a/dask_jobqueue/htcondor.py b/dask_jobqueue/htcondor.py
index fb7b0be7..18fc74eb 100644
--- a/dask_jobqueue/htcondor.py
+++ b/dask_jobqueue/htcondor.py
@@ -16,7 +16,6 @@ class HTCondorJob(Job):
 
 %(job_header)s
 
-Environment = "%(quoted_environment)s"
 Arguments = "%(quoted_arguments)s"
 Executable = %(executable)s
 
@@ -67,7 +66,14 @@ def __init__(
             env_extra = dask.config.get(
                 "jobqueue.%s.env-extra" % self.config_name, default=[]
             )
-        self.env_dict = self.env_lines_to_dict(env_extra)
+
+        if env_extra is not None:
+            # Overwrite command template: prepend commands from env_extra separated by semicolon.
+            # This is special for HTCondor, because lines to execute on the worker node cannot be
+            # simply added to the submit script like for other batch systems.
+            self._command_template = (
+                "; ".join(env_extra) + "; " + self._command_template
+            )
 
         self.job_header_dict = {
             "MY.DaskWorkerName": '"htcondor--$F(MY.JobId)--"',
@@ -118,31 +124,15 @@ def __init__(
             + " ".join(shlex.quote(arg) for arg in cancel_command_extra)
         )
 
-    def env_lines_to_dict(self, env_lines):
-        """Convert an array of export statements (what we get from env-extra
-        in the config) into a dict"""
-        env_dict = {}
-        for env_line in env_lines:
-            split_env_line = shlex.split(env_line)
-            if split_env_line[0] == "export":
-                split_env_line = split_env_line[1:]
-            for item in split_env_line:
-                if "=" in item:
-                    k, v = item.split("=", 1)
-                    env_dict[k] = v
-        return env_dict
-
     def job_script(self):
         """Construct a job submission script"""
         quoted_arguments = quote_arguments(["-c", self._command_template])
-        quoted_environment = quote_environment(self.env_dict)
         job_header_lines = "\n".join(
             "%s = %s" % (k, v) for k, v in self.job_header_dict.items()
         )
         return self._script_template % {
             "shebang": self.shebang,
             "job_header": job_header_lines,
-            "quoted_environment": quoted_environment,
             "quoted_arguments": quoted_arguments,
             "executable": self.executable,
         }
@@ -260,6 +250,17 @@ class HTCondorCluster(JobQueueCluster):
     This also works with adaptive clusters.  This automatically launches and kill workers based on load.
 
     >>> cluster.adapt(maximum_jobs=20)
+
+    If setup commands need to be run before starting the worker on the worker node, ``env_extra`` can be used,
+    e.g., to activate a virtual environment:
+
+    >>> from dask_jobqueue.htcondor import HTCondorCluster
+    >>> cluster = HTCondorCluster(cores=1, memory="2GB", disk="4GB",
+                                  env_extra=['cd /some/path/', 'source venv/bin/activate'])
+
+    Note that environment variables are no longer passed via the ``Environment`` parameter in the submit
+    description file. If you explictly want to set that, you need to use ``job_extra``.
+
     """.format(
         job=job_parameters, cluster=cluster_parameters
     )
diff --git a/dask_jobqueue/tests/test_htcondor.py b/dask_jobqueue/tests/test_htcondor.py
index 99a55733..1664da85 100644
--- a/dask_jobqueue/tests/test_htcondor.py
+++ b/dask_jobqueue/tests/test_htcondor.py
@@ -27,7 +27,12 @@ def test_job_script():
         processes=2,
         memory="100MB",
         disk="100MB",
-        env_extra=['export LANG="en_US.utf8"', 'export LC_ALL="en_US.utf8"'],
+        env_extra=[
+            'export LANG="en_US.utf8"',
+            'export LC_ALL="en_US.utf8"',
+            "cd /some/path/",
+            "source venv/bin/activate",
+        ],
         job_extra={"+Extra": "True"},
         submit_command_extra=["-verbose"],
         cancel_command_extra=["-forcex"],
@@ -40,9 +45,10 @@ def test_job_script():
         assert "MY.DaskWorkerDisk = 100000000" in job_script
         assert "MY.DaskWorkerMemory = 100000000" in job_script
         assert 'MY.JobId = "$(ClusterId).$(ProcId)"' in job_script
-        assert "LANG=en_US.utf8" in job_script
-        assert "LC_ALL=en_US.utf8" in job_script
-        assert "export" not in job_script
+        assert 'export LANG=""en_US.utf8""' in job_script
+        assert 'export LC_ALL=""en_US.utf8""' in job_script
+        assert "cd /some/path/" in job_script
+        assert "source venv/bin/activate" in job_script
         assert "+Extra = True" in job_script
         assert re.search(
             r"condor_submit\s.*-verbose", cluster._dummy_job.submit_command
diff --git a/docs/source/advanced-tips-and-tricks.rst b/docs/source/advanced-tips-and-tricks.rst
index bd798106..237adc6f 100644
--- a/docs/source/advanced-tips-and-tricks.rst
+++ b/docs/source/advanced-tips-and-tricks.rst
@@ -68,6 +68,36 @@ accepted option on some SLURM clusters. The error was something like this:
   sbatch: error: Memory specification can not be satisfied
   sbatch: error: Batch job submission failed: Requested node configuration is not available
 
+Run setup commands before starting the worker with ``env_extra``
+----------------------------------------------------------------
+
+Sometimes you need to run some setup commands before the actual worker can be started. This includes
+setting environment variables, loading environment modules, sourcing/activating a virtual environment,
+or activating conda/mamba environments.
+
+This can be achieved using the ``env_extra`` parameter. Example for setting up a virtual environment:
+
+.. code-block:: python
+
+   from dask_jobqueue.htcondor import HTCondorCluster
+   env_extra = ['cd /some/path', 'source venv/bin/activate']
+   cluster = HTCondorCluster(cores=1, memory="2GB", disk="4GB", log_directory = 'logs', python='python3',
+                             env_extra=env_extra)
+   print(cluster.job_script())
+
+For ``HTCondorCluster``, the commands will be prepended to the actual python call in the ``Arguments``
+parameter in the submit description file. The relevant lines will look like this:
+
+.. code-block:: text
+
+   ...
+   Arguments = "-c 'cd /some/path; source venv/bin/activate; python3 -m distributed.cli.dask_worker tcp://<IP>:<PORT> --nthreads 1 --memory-limit 2.00GB --name dummy-name --nanny --death-timeout 60'"
+   Executable = /bin/sh
+   ...
+
+For other batch systems (``*Cluster`` classes) the additional commands will be inserted as separate lines
+in the submission script.
+
 
 How to handle job queueing system walltime killing workers
 ----------------------------------------------------------
diff --git a/docs/source/examples.rst b/docs/source/examples.rst
index ad029716..4f9a3823 100644
--- a/docs/source/examples.rst
+++ b/docs/source/examples.rst
@@ -32,7 +32,7 @@ PBS Deployments
                         interface='ib0')
 
 Moab Deployments
-~~~~~~~~~~~~~~~~
+----------------
 
 On systems which use the Moab Workload Manager, a subclass of ``PBSCluster``
 can be used, called ``MoabCluster``: