Merge pull request #50 from AllenInstitute/kt_stitch

Kt stitch

Author: RussTorres

acpreprocessing/stitching_modules/acstitch/ccorr_stitch.py

@@ -0,0 +1,25 @@
+import numpy
+from acpreprocessing.stitching_modules.acstitch.rtccorr import get_point_correspondence
+
+
+def get_correspondences(A1_ds,A2_ds,A1_pts,A2_pts,w,r=1,pad=False,cc_threshold=0.8,min_value=0):
+    cc_threshold /= r # TODO: is this the correct rescaling accounting for expanding reference?
+    w = numpy.asarray(w,dtype=int)
+    if len(A1_pts.shape)<2:
+        A1_pts = numpy.array([A1_pts])
+        A2_pts = numpy.array([A2_pts])
+    pm1 = []
+    pm2 = []
+    for p,q in zip(A1_pts.astype(int),A2_pts.astype(int)):
+        A2sub = A2_ds[0,0,(q-r*w)[0]:(q+r*w)[0],(q-r*w)[1]:(q+r*w)[1],(q-r*w)[2]:(q+r*w)[2]]
+        A1sub = A1_ds[0,0,(p-w)[0]:(p+w)[0],(p-w)[1]:(p+w)[1],(p-w)[2]:(p+w)[2]]
+        if r > 1:
+            pw = numpy.asarray([((r-1)*wi,(r-1)*wi) for wi in w],dtype=int)
+            A1sub = numpy.pad(A1sub,pw)
+        p1,p2 = get_point_correspondence(p,q,A1sub,A2sub,autocorrelation_threshold=cc_threshold,padarray=pad,value_threshold=min_value)
+        if not p1 is None:
+            pm1.append(p1)
+            pm2.append(p2)
+    if pm1:
+        return numpy.asarray(pm1),numpy.asarray(pm2)
+    return None,None

acpreprocessing/stitching_modules/acstitch/io.py

@@ -0,0 +1,32 @@
+# -*- coding: utf-8 -*-
+"""
+Created on Mon Jul 31 13:33:46 2023
+
+@author: kevint
+"""
+
+import json
+import gzip
+import numpy
+
+class NumpyEncoder(json.JSONEncoder):
+    def default(self, obj):
+        if isinstance(obj, numpy.ndarray):
+            return obj.tolist()
+        return json.JSONEncoder.default(self, obj)
+    
+    
+def save_pointmatch_file(pmdata,jsonpath):
+    with gzip.open(jsonpath, 'w') as fout:
+        fout.write(json.dumps(pmdata,cls=NumpyEncoder).encode('utf-8'))
+        
+
+def read_pointmatch_file(jsonpath):
+    with gzip.open(jsonpath, 'r') as fin:
+        data = json.loads(fin.read().decode('utf-8'))
+    if data:
+        for tspec in data:
+            for key in ["p_pts","q_pts"]:
+                if not tspec[key] is None:
+                    tspec[key] = numpy.asarray(tspec[key])
+    return data

acpreprocessing/stitching_modules/acstitch/rtccorr.py

@@ -0,0 +1,80 @@
+
+import numpy
+import scipy.ndimage
+
+def correlate_fftns(fft1, fft2):
+    prod = fft1 * fft2.conj()
+    res = numpy.fft.ifftn(prod)
+    
+    corr = numpy.fft.fftshift(res).real
+    return corr
+
+
+def ccorr_fftn(img1, img2):
+    # TODO do we want to pad this?
+    fft1 = numpy.fft.fftn(img1)
+    fft2 = numpy.fft.fftn(img2)
+    
+    return correlate_fftns(fft1, fft2)
+
+
+def autocorr_fftn(img):
+    fft = numpy.fft.fftn(img)
+    return correlate_fftns(fft, fft)
+
+
+def ccorr_and_autocorr_fftn(img1, img2):
+    # TODO do we want to pad this?
+    fft1 = numpy.fft.fftn(img1)
+    fft2 = numpy.fft.fftn(img2)
+    ccorr = correlate_fftns(fft1, fft2)
+    acorr1 = correlate_fftns(fft1, fft1)
+    acorr2 = correlate_fftns(fft2, fft2)
+    return ccorr, acorr1, acorr2
+
+
+def subpixel_maximum(arr):
+    max_loc = numpy.unravel_index(numpy.argmax(arr), arr.shape)
+    
+    sub_arr = arr[
+        tuple(slice(ml-1, ml+2) for ml in max_loc)
+    ]
+    
+    # get center of mass of sub_arr
+    subpixel_max_loc = numpy.array(scipy.ndimage.center_of_mass(sub_arr)) - 1
+    return subpixel_max_loc + max_loc
+
+
+def ccorr_disp(img1, img2, autocorrelation_threshold=None, padarray=False, value_threshold=0):
+    if padarray:
+        d = numpy.ceil(numpy.array(img1.shape) / 2)
+        pw = numpy.asarray([(di,di) for di in d],dtype=int)
+        img1 = numpy.pad(img1,pw)
+        img2 = numpy.pad(img2,pw)
+    if value_threshold:
+        img1[img1<value_threshold] = 0
+        img2[img2<value_threshold] = 0
+    if autocorrelation_threshold is not None:
+        cc, ac1, ac2 = ccorr_and_autocorr_fftn(img1, img2)
+        ac1max = ac1.max()
+        ac2max = ac2.max()
+        if (not numpy.isnan(ac1max) and ac1max > 0) and (not numpy.isnan(ac2max) and ac2max > 0):
+            autocorrelation_ratio = cc.max() / (numpy.sqrt(ac1max*ac2max))
+            if autocorrelation_ratio < autocorrelation_threshold:
+                # what to do here?
+                print("ratio below threshold: " + str(autocorrelation_ratio))
+                return None
+        else:
+            return None
+    else:
+        cc = ccorr_fftn(img1, img2)
+    max_loc = subpixel_maximum(cc)
+    mid_point = numpy.array(img1.shape) // 2
+    return max_loc - mid_point
+
+
+def get_point_correspondence(src_pt, dst_pt, src_patch, dst_patch, autocorrelation_threshold=0.8,padarray=False,value_threshold=0):
+    disp = ccorr_disp(src_patch, dst_patch, autocorrelation_threshold, padarray,value_threshold)
+    if disp is not None:
+        return src_pt, dst_pt - disp
+    return None,None