diff --git a/.github/workflows/ci.yaml b/.github/workflows/ci.yaml index 4a80490b1..5b705a647 100644 --- a/.github/workflows/ci.yaml +++ b/.github/workflows/ci.yaml @@ -7,7 +7,7 @@ on: types: [run-all-workflow-tests-command] env: GALAXY_FORK: galaxyproject - GALAXY_BRANCH: release_22.05 + GALAXY_BRANCH: release_24.0 MAX_CHUNKS: 40 jobs: setup: @@ -23,7 +23,7 @@ jobs: chunk-list: ${{ steps.discover.outputs.chunk-list }} strategy: matrix: - python-version: ['3.7'] + python-version: ['3.11'] steps: - name: Add reaction if: ${{ github.event.client_payload.slash_command.command == 'run-all-workflow-tests' }} @@ -83,7 +83,7 @@ jobs: runs-on: ubuntu-latest strategy: matrix: - python-version: ['3.7'] + python-version: ['3.11'] steps: - uses: actions/checkout@v3 with: @@ -116,7 +116,7 @@ jobs: fail-fast: false matrix: chunk: ${{ fromJson(needs.setup.outputs.chunk-list) }} - python-version: ['3.7'] + python-version: ['3.11'] services: postgres: image: postgres:11 @@ -168,7 +168,7 @@ jobs: needs: [setup, test] strategy: matrix: - python-version: ['3.7'] + python-version: ['3.11'] # This job runs on Linux runs-on: ubuntu-latest steps: diff --git a/.github/workflows/comment_on_pr.yml b/.github/workflows/comment_on_pr.yml new file mode 100644 index 000000000..b186c32e9 --- /dev/null +++ b/.github/workflows/comment_on_pr.yml @@ -0,0 +1,51 @@ +name: Comment on the pull request + +# read-write repo token +# access to secrets +on: + workflow_run: + workflows: ["Galaxy Workflow Tests for push and PR"] + types: + - completed + +jobs: + download_and_comment: + runs-on: ubuntu-latest + if: > + github.event.workflow_run.event == 'pull_request' && + github.event.workflow_run.conclusion == 'failure' + steps: + - name: 'Download artifact' + uses: actions/github-script@v7 + with: + script: | + var artifacts = await github.rest.actions.listWorkflowRunArtifacts({ + owner: context.repo.owner, + repo: context.repo.repo, + run_id: ${{github.event.workflow_run.id }}, + }); + var matchArtifact = artifacts.data.artifacts.filter((artifact) => { + return artifact.name == 'All tool test results' + })[0]; + var download = await github.rest.actions.downloadArtifact({ + owner: context.repo.owner, + repo: context.repo.repo, + artifact_id: matchArtifact.id, + archive_format: 'zip', + }); + var fs = require('fs'); + fs.writeFileSync('${{github.workspace}}/upload.zip', Buffer.from(download.data)); + - run: unzip upload.zip + - name: 'Comment on PR' + uses: actions/github-script@v7 + with: + github-token: ${{ secrets.GITHUB_TOKEN }} + script: | + var fs = require('fs'); + var issue_number = Number(fs.readFileSync('./NR')); + await github.rest.issues.createComment({ + owner: context.repo.owner, + repo: context.repo.repo, + issue_number: issue_number, + body: fs.readFileSync('./tool_test_output.md', 'utf-8') + }); diff --git a/.github/workflows/setup.yml b/.github/workflows/setup.yml index e8f2644e0..3b0968249 100644 --- a/.github/workflows/setup.yml +++ b/.github/workflows/setup.yml @@ -4,17 +4,17 @@ on: inputs: python-version-list: description: 'stringified JSON array of Python versions' - default: "[\"3.7\"]" + default: "[\"3.11\"]" required: false type: string galaxy-fork: description: 'Galaxy fork to use' - default: '' + default: galaxyproject required: false type: string galaxy-branch: description: 'Galaxy branch to use' - default: '' + default: master required: false type: string max-chunks: diff --git a/.github/workflows/test_workflows.yml b/.github/workflows/test_workflows.yml index e672220d3..073e37fc5 100644 --- a/.github/workflows/test_workflows.yml +++ b/.github/workflows/test_workflows.yml @@ -18,7 +18,7 @@ on: type: string python-version-list: description: 'stringified JSON array of Python versions' - default: "[\"3.7\"]" + default: "[\"3.11\"]" required: false type: string repository-list: @@ -28,12 +28,12 @@ on: type: string galaxy-fork: description: 'Galaxy fork to use' - default: '' + default: galaxyproject required: false type: string galaxy-branch: description: 'Galaxy branch to use' - default: '' + default: master required: false type: string check-outputs: diff --git a/.github/workflows/workflow_test.yml b/.github/workflows/workflow_test.yml index 0adb35dca..99918de07 100644 --- a/.github/workflows/workflow_test.yml +++ b/.github/workflows/workflow_test.yml @@ -9,10 +9,10 @@ jobs: name: Setup cache and determine changed repositories uses: ./.github/workflows/setup.yml with: - python-version-list: "[\"3.7\"]" - galaxy-fork: galaxyproject - galaxy-branch: release_22.05 + python-version-list: "[\"3.11\"]" max-chunks: 4 + galaxy-fork: galaxyproject + galaxy-branch: release_24.0 # Planemo lint the changed repositories lint: @@ -22,7 +22,7 @@ jobs: runs-on: ubuntu-latest strategy: matrix: - python-version: ['3.7'] + python-version: ['3.11'] steps: - uses: actions/checkout@v3 with: @@ -54,10 +54,10 @@ jobs: galaxy-head-sha: ${{ needs.setup.outputs.galaxy-head-sha }} chunk-count: ${{ fromJSON(needs.setup.outputs.chunk-count) }} chunk-list: ${{ needs.setup.outputs.chunk-list }} - python-version-list: "[\"3.7\"]" + python-version-list: "[\"3.11\"]" repository-list: ${{ needs.setup.outputs.repository-list }} galaxy-fork: galaxyproject - galaxy-branch: master + galaxy-branch: release_24.0 check-outputs: false combine_outputs: @@ -66,7 +66,7 @@ jobs: needs: [setup, test] strategy: matrix: - python-version: ['3.7'] + python-version: ['3.11'] runs-on: ubuntu-latest steps: - uses: actions/download-artifact@v3 @@ -87,6 +87,13 @@ jobs: with: mode: combine html-report: true + markdown-report: true + - run: cat upload/tool_test_output.md >> $GITHUB_STEP_SUMMARY + - name: Save PR number + run: echo ${{ github.event.number }} > ./upload/NR + - name: Debug PR number + run: | + cat ./upload/NR - uses: actions/upload-artifact@v3 with: name: 'All tool test results' @@ -103,7 +110,7 @@ jobs: needs: [setup,test,combine_outputs] strategy: matrix: - python-version: ['3.7'] + python-version: ['3.11'] runs-on: ubuntu-latest if: github.ref == 'refs/heads/main' && github.repository_owner == 'galaxyproject' steps: @@ -139,3 +146,25 @@ jobs: workflows: true workflow-namespace: iwc-workflows github-token: ${{ secrets.IWC_WORKFLOWS_BOT_TOKEN }} + + deploy-report: + name: Report deploy status + needs: [deploy] + if: ${{ always() && needs.deploy.result != 'success' && (github.ref == 'refs/heads/master' || github.ref == 'refs/heads/main' ) && github.repository_owner == 'galaxyproject' }} + runs-on: ubuntu-latest + steps: + # report to the PR if deployment failed + - name: Get PR object + uses: 8BitJonny/gh-get-current-pr@2.2.0 + id: getpr + with: + sha: ${{ github.event.after }} + - name: Create comment + uses: peter-evans/create-or-update-comment@v3 + with: + token: ${{ secrets.PAT }} + issue-number: ${{ steps.getpr.outputs.number }} + body: | + Attention: deployment ${{ needs.deploy.result }}! + + https://github.com/${{ github.repository }}/actions/runs/${{ github.run_id }} diff --git a/static/update_workflow.png b/static/update_workflow.png new file mode 100644 index 000000000..04d8b79bf Binary files /dev/null and b/static/update_workflow.png differ diff --git a/workflows/README.md b/workflows/README.md index fc27a175a..f7099320c 100644 --- a/workflows/README.md +++ b/workflows/README.md @@ -8,11 +8,12 @@ The structure is as follows: * top-level directories represent categories, e.g., `sars-cov-2-variant-calling`; * directories right under the top level represent individual workflow repositories, e.g., `sars-cov-2-consensus-from-variation` which will be deployed to https://github.com/iwc-workflows/sars-cov-2-consensus-from-variation; +* please, only use lower case and `-` in directory names. * workflow repository directories contain: * the `.ga` workflow file, e.g., `consensus-from-variation.ga`; - * a [Planemo test file](https://planemo.readthedocs.io/en/latest/test_format.html), with the same name as the workflow file, but with a `-test.yml` extension, e.g., `consensus-from-variation-test.yml`; - * a `test-data` directory with the test data used by Planemo; + * a [Planemo test file](https://planemo.readthedocs.io/en/latest/test_format.html), with the same name as the workflow file, but with a `-tests.yml` extension, e.g., `consensus-from-variation-tests.yml`; + * a `test-data` directory with the test data used by Planemo (optional); * a [Dockstore](https://dockstore.org) [metadata file](https://docs.dockstore.org/en/develop/getting-started/github-apps/github-apps.html#workflow-yml-file) named `.dockstore.yml`; * a `README.md` and a `CHANGELOG.md` file. @@ -20,23 +21,25 @@ The structure is as follows: Here are some guidelines to help new contributors to add their workflows. -Everything starts from a workflow that you need to download locally. - -Currently the CI is using CVMFS for tools and for references, this is why all tests described below are against usegalaxy.org. +Everything starts from a workflow that you have on a galaxy instance. ### Optional: Updating tools -It is recommended to check and update tools with Planemo before import : +It is recommended to check and update tools with Planemo before import. -```bash -planemo autoupdate -``` + - You can do it from the galaxy instance using "Workflow Options" "Upgrade Workflow": + + ![Update galaxy workflow in galaxy](../static/update_workflow.png) + + - Or by downloading the ga file and running planemo: -Then, it is recommended to upload this updated workflow to the usegalaxy.org server as some updates may break connections (and some tools may not yet be installed on usegalaxy.org). + ```bash + planemo autoupdate + ``` ### Ensure workflows follow best-practices -Start by opening your workflow in the workflow editor, click on "Workflow Options", select "Best Practices" and apply the suggested improvements. In particular, in order to make the workflow usable, **make sure you specify a license**. Another important field is "Creator", which allows to give proper credit to the author(s). The "Identifier" field of a Creator added as a _person_ should be filled with a fully qualified URI, e.g., https://orcid.org/0000-0002-1825-0097. +The best way is to check it into a galaxy instance: click on "Workflow Options", select "Best Practices" and apply the suggested improvements. In particular, in order to make the workflow usable, **make sure you specify a license**. Another important field is "Creator", which allows to give proper credit to the author(s). The "Identifier" field of a Creator added as a _person_ should be filled with a fully qualified URI, e.g., https://orcid.org/0000-0002-1825-0097. ![Add Creator GIF](../static/add-creator.gif) @@ -45,16 +48,17 @@ If you add an _organization_ as Creator, you should include a "URL" field pointi ### Generate tests -You can either write test cases by hand, or use a workflow invocation to generate a test case: +This is usually the most difficult part and we encourage all new contributors to IWC to propose their workflows even if they did not managed to generate tests. However, the publication of these workflow will be speed up if tests are already present. +To generate tests, you can either write test cases by hand, or use a workflow invocation to generate a test case (see below). #### Find input datasets -If your analysis is covered by the GTN, using GTN dataset material is a good start! +If your analysis is covered by the GTN, using GTN dataset material is a good start! If it is not, try to generate a toy dataset for example following [this](https://training.galaxyproject.org/training-material/topics/contributing/tutorials/create-new-tutorial/tutorial.html#get-a-toy-dataset) and then publish it to zenodo to have a permanent URL. #### Generate test from a workflow invocation Create a new directory under one of the directories that represent categories. If no category is suitable you can create a new category directory. -Name the directory that contains your workflow(s) appropriately, as it will become the name of the repository deployed to [iwc-workflows github organization](https://github.com/iwc-workflows). +Name the directory that contains your workflow(s) appropriately, as it will become the name of the repository deployed to [iwc-workflows github organization](https://github.com/iwc-workflows). Only use lower-case and `-` in names of categories and repositories. Execute the workflow on your Galaxy server using the smallest input data you can generate. Go to the workflow invocations page (User > Workflow Invocations), open the most recent item and find the invocation id: @@ -64,26 +68,27 @@ Go to the workflow invocations page (User > Workflow Invocations), open the most You will also need your Galaxy API key. To copy it, or generate it if you don't have one yet, go to User > Preferences > Manage API Key. Then run: ``` -planemo workflow_test_init --from_invocation --galaxy_url https://usegalaxy.org/ --galaxy_user_key +planemo workflow_test_init --from_invocation --galaxy_url --galaxy_user_key ``` This will place the workflow and workflow test files in your current working directory. You may still want to remove test files and edit the test comparisons so that tests will pass reliably. For example, you could consider using assertions to test the outputs, rather than comparing the entire output file with test data. +Also, if some outputs are large, it is better to use assertions than storing the whole output file to the iwc repository. #### Manually write test for workflow Download the workflow and place it in a new directory under one of the directories that represent categories. If no category is suitable you can create a new category directory. -Name the directory that contains your workflow(s) appropriately, as it will become the name of the repository deployed to [iwc-workflows github organization](https://github.com/iwc-workflows). -You can then generate a template planemo test file. Here we generate a test file for the workflow parallel-accession-download.ga. +Name the directory that contains your workflow(s) appropriately, as it will become the name of the repository deployed to [iwc-workflows github organization](https://github.com/iwc-workflows). Only use lower-case and `-` in names of categories and repositories. +You can then generate a template planemo test file with planemo: ```bash -planemo workflow_test_init parallel-accession-download.ga +planemo workflow_test_init ``` -This generates the file parallel-accession-download-tests.yml: +If you run it on `parallel-accession-download.ga`, this generates the file parallel-accession-download-tests.yml: ```yaml - doc: Test outline for parallel-accession-download.ga @@ -98,7 +103,7 @@ This generates the file parallel-accession-download-tests.yml: class: '' ``` -We now need to provide inputs and outputs. You can find details about the test format in the [planemo documentation](https://planemo.readthedocs.io/en/latest/test_format.html). +We now need to provide inputs and outputs. You can find details about the test format in the [planemo documentation](https://planemo.readthedocs.io/en/latest/test_format.html). You can also check other test files into this repository to get examples. For this example workflow the final parallel-accession-download-tests.yml might look like this: ```yaml @@ -127,14 +132,20 @@ Applying linter tests... CHECK .. CHECK: Tests appear structurally correct for parallel-accession-download.ga ``` -#### Test your workflow against usegalaxy.org +#### Test your workflow against an instance which have all tools installed. -Before running the CI, it might be interesting to run your tests against usegalaxy.org using planemo: +Before running the CI, it might be interesting to run your tests against a galaxy instance using planemo so you can easily see what is failing and what are the differences between your expectations and the output you get: ```bash -planemo test --galaxy_url https://usegalaxy.org/ --galaxy_user_key +planemo test --galaxy_url --galaxy_user_key ``` +If your tests are not passing because you made an error into your test file you can modify your test file and use planemo to check that the test is valid against the same invocation. + +planemo workflow_test_on_invocation --galaxy_url + +Note: If your workflow is using build-in indexes, note that the CI will use CVMFS. You can browse the available indexes at http://datacache.galaxyproject.org/. + #### Add required metadata We now need to generate a `.dockstore.yml` file that contains metadata needed for [Dockstore](https://dockstore.org/organizations/iwc). diff --git a/workflows/VGP-assembly-v2/Assembly-Hifi-HiC-phasing-VGP4/.dockstore.yml b/workflows/VGP-assembly-v2/Assembly-Hifi-HiC-phasing-VGP4/.dockstore.yml new file mode 100644 index 000000000..0622300d2 --- /dev/null +++ b/workflows/VGP-assembly-v2/Assembly-Hifi-HiC-phasing-VGP4/.dockstore.yml @@ -0,0 +1,14 @@ +version: 1.2 +workflows: +- name: main + subclass: Galaxy + publish: true + primaryDescriptorPath: /Assembly-Hifi-HiC-phasing-VGP4.ga + testParameterFiles: + - /Assembly-Hifi-HiC-phasing-VGP4-tests.yml + authors: + - name: Galaxy + - name: VGP + url: https://vertebrategenomeproject.org + - name: Delphine Lariviere + orcid: 0000-0001-6421-3484 diff --git a/workflows/VGP-assembly-v2/Assembly-Hifi-HiC-phasing-VGP4/Assembly-Hifi-HiC-phasing-VGP4-tests.yml b/workflows/VGP-assembly-v2/Assembly-Hifi-HiC-phasing-VGP4/Assembly-Hifi-HiC-phasing-VGP4-tests.yml new file mode 100644 index 000000000..7d9d57906 --- /dev/null +++ b/workflows/VGP-assembly-v2/Assembly-Hifi-HiC-phasing-VGP4/Assembly-Hifi-HiC-phasing-VGP4-tests.yml @@ -0,0 +1,56 @@ +- doc: Test outline for Assembly-Hifi-HiC-phasing-VGP4 + job: + HiC forward reads: + class: File + location: https://zenodo.org/records/10068595/files/HiC%20forward%20reads.fastqsanger.gz?download=1 + filetype: fastqsanger.gz + HiC reverse reads: + class: File + location: https://zenodo.org/records/10068595/files/HiC%20reverse%20reads.fastqsanger.gz?download=1 + filetype: fastqsanger.gz + Genomescope Model Parameters: + class: File + path: test-data/Genomescope Model Parameters.tabular + filetype: tabular + Genomescope Summary: + class: File + location: https://zenodo.org/records/10068595/files/Genomescope%20Summary.txt?download=1 + filetype: txt + Meryl Database: + class: File + location: https://zenodo.org/records/10068595/files/Meryl%20Database.meryldb?download=1 + filetype: meryldb + Pacbio Reads Collection: + class: Collection + collection_type: list + elements: + - class: File + identifier: yeast_reads_sub1.fastq.gz + location: https://zenodo.org/records/10068595/files/Pacbio%20Reads%20Collection_yeast_reads_sub1.fastq.gz.fastq.gz?download=1 + Lineage: vertebrata_odb10 + Bits for bloom filter: 32 + Name for Haplotype 1: Hap1 + Name for Haplotype 2: Hap2 + outputs: + Hifiasm HiC hap1: + assert: + has_n_line: + n: 168 + Estimated Genome size: 2288021 + Busco Summary Hap1: + asserts: + has_text: + text: "C:1.0%[S:1.0%,D:0.0%],F:0.4%,M:98.6%,n:3354" + Nx Plot: + asserts: + has_size: + value: 65000 + delta: 10000 + usable hap1 gfa: + assert: + has_n_line: + n: 173 + Assembly statistics fir Hap1 and Hap2: + asserts: + has_text: + text: "Metric hap1 hap2" diff --git a/workflows/VGP-assembly-v2/Assembly-Hifi-HiC-phasing-VGP4/Assembly-Hifi-HiC-phasing-VGP4.ga b/workflows/VGP-assembly-v2/Assembly-Hifi-HiC-phasing-VGP4/Assembly-Hifi-HiC-phasing-VGP4.ga new file mode 100644 index 000000000..ca159e43b --- /dev/null +++ b/workflows/VGP-assembly-v2/Assembly-Hifi-HiC-phasing-VGP4/Assembly-Hifi-HiC-phasing-VGP4.ga @@ -0,0 +1,3369 @@ +{ + "a_galaxy_workflow": "true", + "annotation": "", + "creator": [ + { + "class": "Organization", + "name": "Galaxy" + }, + { + "class": "Organization", + "name": "VGP", + "url": "https://vertebrategenomeproject.org" + }, + { + "class": "Person", + "identifier": "https://orcid.org/0000-0001-6421-3484", + "name": "Delphine Lariviere" + } + ], + "format-version": "0.1", + "license": "CC-BY-4.0", + "release": "0.1.8", + "name": "Assembly-Hifi-HiC-phasing-VGP4", + "steps": { + "0": { + "annotation": "A simple list containing PacBio data in either fasta or fastq formats.", + "content_id": null, + "errors": null, + "id": 0, + "input_connections": {}, + "inputs": [ + { + "description": "A simple list containing PacBio data in either fasta or fastq formats.", + "name": "Pacbio Reads Collection" + } + ], + "label": "Pacbio Reads Collection", + "name": "Input dataset collection", + "outputs": [], + "position": { + "left": 0, + "top": 54.86661965562355 + }, + "tool_id": null, + "tool_state": "{\"optional\": false, \"tag\": null, \"collection_type\": \"list\"}", + "tool_version": null, + "type": "data_collection_input", + "uuid": "f7ef62fa-eb54-4489-b444-b6be262a45c0", + "when": null, + "workflow_outputs": [] + }, + "1": { + "annotation": "Forward reads as a single dataset in fastq format", + "content_id": null, + "errors": null, + "id": 1, + "input_connections": {}, + "inputs": [ + { + "description": "Forward reads as a single dataset in fastq format", + "name": "HiC forward reads" + } + ], + "label": "HiC forward reads", + "name": "Input dataset", + "outputs": [], + "position": { + "left": 5.227635768494666, + "top": 146.68167273687337 + }, + "tool_id": null, + "tool_state": "{\"optional\": false, \"tag\": null}", + "tool_version": null, + "type": "data_input", + "uuid": "d5d37559-d0fd-4d56-bb2f-a89335984a73", + "when": null, + "workflow_outputs": [] + }, + "2": { + "annotation": "Reverse reads as a single dataset in fastq format", + "content_id": null, + "errors": null, + "id": 2, + "input_connections": {}, + "inputs": [ + { + "description": "Reverse reads as a single dataset in fastq format", + "name": "HiC reverse reads" + } + ], + "label": "HiC reverse reads", + "name": "Input dataset", + "outputs": [], + "position": { + "left": 14.512079824566827, + "top": 240.65230620636626 + }, + "tool_id": null, + "tool_state": "{\"optional\": false, \"tag\": \"\"}", + "tool_version": null, + "type": "data_input", + "uuid": "94594456-f087-4983-9b0e-014e36084354", + "when": null, + "workflow_outputs": [] + }, + "3": { + "annotation": "GenomeScope summary generated by K-mer profiling workflow", + "content_id": null, + "errors": null, + "id": 3, + "input_connections": {}, + "inputs": [ + { + "description": "GenomeScope summary generated by K-mer profiling workflow", + "name": "Genomescope Summary" + } + ], + "label": "Genomescope Summary", + "name": "Input dataset", + "outputs": [], + "position": { + "left": 18.350006103515625, + "top": 338.7749938964844 + }, + "tool_id": null, + "tool_state": "{\"optional\": false, \"tag\": \"\"}", + "tool_version": null, + "type": "data_input", + "uuid": "1844cbb3-ba5f-4997-a3cd-ce70f4570a99", + "when": null, + "workflow_outputs": [] + }, + "4": { + "annotation": "GenomeScope model parameters generated by K-mer profiling workflow", + "content_id": null, + "errors": null, + "id": 4, + "input_connections": {}, + "inputs": [ + { + "description": "GenomeScope model parameters generated by K-mer profiling workflow", + "name": "Genomescope Model Parameters" + } + ], + "label": "Genomescope Model Parameters", + "name": "Input 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`toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_grep_tool/9.3+galaxy0` +- `toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_replace_in_line/1.1.2` was updated to `toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_replace_in_line/9.3+galaxy0` +- `toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_awk_tool/1.1.2` was updated to `toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_awk_tool/9.3+galaxy0` +- `toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_sed_tool/1.1.1` was updated to `toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_sed_tool/9.3+galaxy0` +- `toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_cat/0.1.1` was updated to `toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_cat/9.3+galaxy0` +- `toolshed.g2.bx.psu.edu/repos/lparsons/cutadapt/cutadapt/4.4+galaxy0` was updated to `toolshed.g2.bx.psu.edu/repos/lparsons/cutadapt/cutadapt/4.7+galaxy0` +- `toolshed.g2.bx.psu.edu/repos/bgruening/hifiasm/hifiasm/0.19.8+galaxy0` was updated to `toolshed.g2.bx.psu.edu/repos/bgruening/hifiasm/hifiasm/0.19.8+galaxy1` + +## [0.1.7] 2023-11-20 + +- Fix author in dockstore + +## [0.1.5] 2023-11-15 + +### Added + +- more descriptive labels for inputs + +### Automatic update +- `toolshed.g2.bx.psu.edu/repos/iuc/busco/busco/5.4.6+galaxy0` was updated to `toolshed.g2.bx.psu.edu/repos/iuc/busco/busco/5.5.0+galaxy0` + +## [0.1.4] 2023-11-08 + +### Automatic update +- `toolshed.g2.bx.psu.edu/repos/lparsons/cutadapt/cutadapt/4.0+galaxy1` was updated to `toolshed.g2.bx.psu.edu/repos/lparsons/cutadapt/cutadapt/4.4+galaxy0` +- `toolshed.g2.bx.psu.edu/repos/bgruening/hifiasm/hifiasm/0.16.1+galaxy4` was updated to `toolshed.g2.bx.psu.edu/repos/bgruening/hifiasm/hifiasm/0.19.8+galaxy0` +- `toolshed.g2.bx.psu.edu/repos/iuc/datamash_ops/datamash_ops/1.1.0+galaxy2` was updated to `toolshed.g2.bx.psu.edu/repos/iuc/datamash_ops/datamash_ops/1.8+galaxy0` +- `toolshed.g2.bx.psu.edu/repos/iuc/busco/busco/5.3.2+galaxy0` was updated to `toolshed.g2.bx.psu.edu/repos/iuc/busco/busco/5.4.6+galaxy0` +- `toolshed.g2.bx.psu.edu/repos/iuc/merqury/merqury/1.3+galaxy2` was updated to `toolshed.g2.bx.psu.edu/repos/iuc/merqury/merqury/1.3+galaxy3` +- `toolshed.g2.bx.psu.edu/repos/devteam/add_value/addValue/1.0.0` was updated to `toolshed.g2.bx.psu.edu/repos/devteam/add_value/addValue/1.0.1` +- `toolshed.g2.bx.psu.edu/repos/iuc/ggplot2_point/ggplot2_point/3.4.0+galaxy0` was updated to `toolshed.g2.bx.psu.edu/repos/iuc/ggplot2_point/ggplot2_point/3.4.0+galaxy1` + +## [0.1.3] - 2023-11-08 + +- Added tags `hifiasm_Assembly_Haplotype_1` and `hifiasm_Assembly_Haplotype_2` to fasta outputs of hifiasm, so they can be easily identified for feeding to purge_dups workflow +- Made BUSCO lineages selectable from workflow launch interface + +## [0.1.2] - 2023-11-07 + +- Added input filtering based on tags. Rearranged input ordering. + +## [0.1] - 2023-09-27 + +- Creation of workflow for HiFi assembly with Hi-C phasing. diff --git a/workflows/VGP-assembly-v2/Assembly-Hifi-HiC-phasing-VGP4/README.md b/workflows/VGP-assembly-v2/Assembly-Hifi-HiC-phasing-VGP4/README.md new file mode 100644 index 000000000..1cc40e5db --- /dev/null +++ b/workflows/VGP-assembly-v2/Assembly-Hifi-HiC-phasing-VGP4/README.md @@ -0,0 +1,23 @@ +# Contiging Solo w/HiC: + +Generate phased assembly based on PacBio Hifi Reads using HiC data from the same individual for phasing. + +## Inputs + +1. Hifi long reads [fastq] +2. HiC forward reads (if multiple input files, concatenated in same order as reverse reads) [fastq] +3. HiC reverse reads (if multiple input files, concatenated in same order as forward reads) [fastq] +4. K-mer database [meryldb] +5. Genome profile summary generated by Genomescope [txt] +6. Name of first assembly +7. Name of second assembly + +## Outputs + +1. Haplotype 1 assembly ([fasta] and [gfa]) +2. Haplotype 2 assembly ([fasta] and [gfa]) +3. QC: BUSCO report for both assemblies +4. QC: Merqury report for both assemblies +5. QC: Assembly statistics for both assemblies +6. QC: Nx plot for both assemblies +7. 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Database : Maternal': + class: File + location: https://zenodo.org/records/10056319/files/Hapmer%20Database%20-%20Maternal.meryldb?download=1 + filetype: meryldb + Genomescope Summary: + class: File + location: https://zenodo.org/records/10056319/files/Genomescope%20Summary.txt?download=1 + filetype: txt + 'Pacbio Reads Collection : child': + class: Collection + collection_type: list + elements: + - class: File + identifier: yeast_reads_sub1.fastq.gz + location: https://zenodo.org/records/10056319/files/Pacbio%20Reads%20Collection.fastq.gz?download=1 + Paternal Illumina reads (hap1): + class: Collection + collection_type: list + elements: + - class: File + identifier: hap1_2.fq + location: https://zenodo.org/records/10056319/files/Sub_hap1_2.fastqsanger?download=1 + - class: File + identifier: hap1_1.fq + location: https://zenodo.org/records/10056319/files/Sub_hap1_1.fastqsanger?download=1 + Maternal Illumina reads (hap2): + class: Collection + collection_type: list + elements: + - 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`toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_replace_in_line/9.3+galaxy0` +- `toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_awk_tool/1.1.2` was updated to `toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_awk_tool/9.3+galaxy0` +- `toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_sed_tool/1.1.1` was updated to `toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_sed_tool/9.3+galaxy0` +- `toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_cat/0.1.1` was updated to `toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_cat/9.3+galaxy0` +- `toolshed.g2.bx.psu.edu/repos/lparsons/cutadapt/cutadapt/4.4+galaxy0` was updated to `toolshed.g2.bx.psu.edu/repos/lparsons/cutadapt/cutadapt/4.7+galaxy0` +- `toolshed.g2.bx.psu.edu/repos/bgruening/hifiasm/hifiasm/0.19.8+galaxy0` was updated to `toolshed.g2.bx.psu.edu/repos/bgruening/hifiasm/hifiasm/0.19.8+galaxy1` + +## [0.1.2] 2023-11-14 + +### Automatic update +- `toolshed.g2.bx.psu.edu/repos/lparsons/cutadapt/cutadapt/4.0+galaxy1` was updated to `toolshed.g2.bx.psu.edu/repos/lparsons/cutadapt/cutadapt/4.4+galaxy0` +- `toolshed.g2.bx.psu.edu/repos/bgruening/hifiasm/hifiasm/0.16.1+galaxy4` was updated to `toolshed.g2.bx.psu.edu/repos/bgruening/hifiasm/hifiasm/0.19.8+galaxy0` +- `toolshed.g2.bx.psu.edu/repos/iuc/busco/busco/5.3.2+galaxy0` was updated to `toolshed.g2.bx.psu.edu/repos/iuc/busco/busco/5.4.6+galaxy0` +- `toolshed.g2.bx.psu.edu/repos/iuc/merqury/merqury/1.3+galaxy2` was updated to `toolshed.g2.bx.psu.edu/repos/iuc/merqury/merqury/1.3+galaxy3` +- `toolshed.g2.bx.psu.edu/repos/iuc/datamash_ops/datamash_ops/1.1.0+galaxy2` was updated to `toolshed.g2.bx.psu.edu/repos/iuc/datamash_ops/datamash_ops/1.8+galaxy0` +- `toolshed.g2.bx.psu.edu/repos/devteam/add_value/addValue/1.0.0` was updated to `toolshed.g2.bx.psu.edu/repos/devteam/add_value/addValue/1.0.1` +- `toolshed.g2.bx.psu.edu/repos/iuc/ggplot2_point/ggplot2_point/3.4.0+galaxy0` was updated to `toolshed.g2.bx.psu.edu/repos/iuc/ggplot2_point/ggplot2_point/3.4.0+galaxy1` + +## [0.1.1] 2023-11-20 + +- Fix author in dockstore + + +## [0.1] - 2023-09-27 + +Creation of workflow for Trio assembly. + diff --git a/workflows/VGP-assembly-v2/Assembly-Hifi-Trio-phasing-VGP5/README.md b/workflows/VGP-assembly-v2/Assembly-Hifi-Trio-phasing-VGP5/README.md new file mode 100644 index 000000000..f9d85ac45 --- /dev/null +++ b/workflows/VGP-assembly-v2/Assembly-Hifi-Trio-phasing-VGP5/README.md @@ -0,0 +1,28 @@ +# Assembly with Hifi reads and Trio Data + +Generate phased assembly based on PacBio Hifi Reads using parental Illumina data for phasing + +## Inputs + +1. Hifi long reads [fastq] +2. Concatenated Illumina reads : Paternal [fastq] +3. Concatenated Illumina reads : Maternal [fastq] +4. K-mer database [meryldb] +5. Paternal hapmer database [meryldb] +6. Maternal hapmer database [meryldb] +7. Genome profile summary generated by Genomescope [txt] +8. Bloom Filter +9. Name of first haplotype +10. Name of second haplotype + +## Outputs + +1. Haplotype 1 assembly +2. Haplotype 2 assembly +3. QC: BUSCO report for both assemblies +4. Merqury report for both assemblies +5. Assembly statistics for both assemblies +6. Nx Plot for both assemblies +7. Size plot for both assemblies + + diff --git a/workflows/VGP-assembly-v2/Assembly-Hifi-only-VGP3/.dockstore.yml b/workflows/VGP-assembly-v2/Assembly-Hifi-only-VGP3/.dockstore.yml new file mode 100644 index 000000000..78eb01153 --- /dev/null +++ b/workflows/VGP-assembly-v2/Assembly-Hifi-only-VGP3/.dockstore.yml @@ -0,0 +1,12 @@ +version: 1.2 +workflows: +- name: main + subclass: Galaxy + publish: true + primaryDescriptorPath: /Assembly-Hifi-only-VGP3.ga + testParameterFiles: + - /Assembly-Hifi-only-VGP3-tests.yml + authors: + - name: Galaxy + - name: VGP + url: https://vertebrategenomeproject.org diff --git a/workflows/VGP-assembly-v2/Assembly-Hifi-only-VGP3/Assembly-Hifi-only-VGP3-tests.yml b/workflows/VGP-assembly-v2/Assembly-Hifi-only-VGP3/Assembly-Hifi-only-VGP3-tests.yml new file mode 100644 index 000000000..627a008f3 --- /dev/null +++ b/workflows/VGP-assembly-v2/Assembly-Hifi-only-VGP3/Assembly-Hifi-only-VGP3-tests.yml @@ -0,0 +1,45 @@ +- doc: Test for Assembly-Hifi-only-VGP3 + job: + Meryl Database: + class: File + location: https://zenodo.org/record/8371053/files/Meryl-db.meryldb?download=1 + filetype: meryldb + Genomescope Summary: + class: File + location: https://zenodo.org/record/8371053/files/GenomeScope_Summary.txt?download=1 + filetype: txt + Pacbio Reads Collection: + class: Collection + collection_type: list + elements: + - class: File + identifier: yeast_reads_sub1.fastq.gz + location: https://zenodo.org/record/8371053/files/yeast_reads_sub1.fastq.gz?download=1 + filetype: fastqsanger.gz + Bits for Hifiasm bloom filter: 32 + SAK input file (Optional): null + Name of primary assembly: Primary + Name of alternate assembly: Alternate + outputs: + Hifiasm Primary assembly: + asserts: + has_n_lines: + n: 170 + Hifiasm Alternate assembly: + asserts: + has_n_lines: + n: 2 + Assembly Stats on Alternate Assembly: + asserts: + has_line: + line: "# scaffolds 1" + Estimated Genome size: 2288021 + Assembly Stats on Primary assembly: + asserts: + has_line: + line: "# scaffolds 85" + Nx Plot: + asserts: + has_size: + value : 60000 + delta: 10000 diff --git a/workflows/VGP-assembly-v2/Assembly-Hifi-only-VGP3/Assembly-Hifi-only-VGP3.ga b/workflows/VGP-assembly-v2/Assembly-Hifi-only-VGP3/Assembly-Hifi-only-VGP3.ga new file mode 100644 index 000000000..b334081f6 --- /dev/null +++ b/workflows/VGP-assembly-v2/Assembly-Hifi-only-VGP3/Assembly-Hifi-only-VGP3.ga @@ -0,0 +1,2863 @@ +{ + "a_galaxy_workflow": "true", + "annotation": "", + "creator": [ + { + "class": "Organization", + "name": "Galaxy" + }, + { + "class": "Organization", + "name": "VGP", + "url": "https://vertebrategenomeproject.org" + } + ], + "format-version": "0.1", + "license": "CC-BY-4.0", + "release": "0.1.3", + "name": "Assembly-Hifi-only-VGP3", + "steps": { + "0": { + "annotation": "", + "content_id": null, + "errors": null, + "id": 0, + "input_connections": {}, + "inputs": [ + { + "description": "", + "name": "Pacbio Reads Collection" + } + ], + "label": "Pacbio Reads Collection", + "name": "Input dataset collection", + "outputs": [], + "position": { + "left": 0, + "top": 684.5727767944336 + }, + "tool_id": null, + "tool_state": "{\"optional\": false, \"tag\": \"\", \"collection_type\": \"list\"}", + "tool_version": null, + "type": "data_collection_input", + "uuid": "188272eb-651c-4cad-9cdc-57dc6425947d", + "when": null, + "workflow_outputs": [] + }, + "1": { + "annotation": "", + "content_id": null, + "errors": null, + "id": 1, + "input_connections": {}, + "inputs": [ + { + "description": "", + "name": "Meryl Database" + } + ], + "label": "Meryl Database", + "name": "Input dataset", + "outputs": [], + "position": { + "left": 24.8125, + "top": 1200.546875 + }, + "tool_id": null, + "tool_state": "{\"optional\": false, \"tag\": \"\"}", + "tool_version": null, + "type": "data_input", + "uuid": "a3b31a30-b60f-4e4a-96d1-f4c0513ef476", + "when": null, + "workflow_outputs": [] + }, + "2": { + "annotation": "", + "content_id": null, + "errors": null, + "id": 2, + "input_connections": {}, + "inputs": [ + { + "description": "", + "name": "Genomescope Summary" + } + ], + "label": "Genomescope Summary", + "name": "Input dataset", + "outputs": [], + "position": { + "left": 13.59375, + "top": 1344.075584411621 + }, + "tool_id": null, + "tool_state": "{\"optional\": false, \"tag\": \"\"}", + "tool_version": null, + "type": "data_input", + "uuid": "705c22f0-68e3-4f5e-93da-e1dda2a80f98", + "when": null, + "workflow_outputs": [] + }, + "3": { + "annotation": "Defaults to 37 if not specified. 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b/workflows/VGP-assembly-v2/Assembly-Hifi-only-VGP3/CHANGELOG.md @@ -0,0 +1,40 @@ +# Changelog + + +## [0.1.3] 2024-03-25 + +### Manual Updates +- Add Gff outputs to Busco +- Add histogram outputs to Merqury +- Add labels to subworkflows + + +### Automatic update +- `toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_grep_tool/1.1.1` was updated to `toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_grep_tool/9.3+galaxy0` +- `toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_replace_in_line/1.1.2` was updated to `toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_replace_in_line/9.3+galaxy0` +- `toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_awk_tool/1.1.2` was updated to `toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_awk_tool/9.3+galaxy0` +- `toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_sed_tool/1.1.1` was updated to `toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_sed_tool/9.3+galaxy0` +- `toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_cat/0.1.1` was updated to `toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_cat/9.3+galaxy0` +- `toolshed.g2.bx.psu.edu/repos/lparsons/cutadapt/cutadapt/4.4+galaxy0` was updated to `toolshed.g2.bx.psu.edu/repos/lparsons/cutadapt/cutadapt/4.7+galaxy0` +- `toolshed.g2.bx.psu.edu/repos/bgruening/hifiasm/hifiasm/0.19.8+galaxy0` was updated to `toolshed.g2.bx.psu.edu/repos/bgruening/hifiasm/hifiasm/0.19.8+galaxy1` + + +## [0.1.2] 2023-11-14 + +### Automatic update +- `toolshed.g2.bx.psu.edu/repos/lparsons/cutadapt/cutadapt/4.0+galaxy1` was updated to `toolshed.g2.bx.psu.edu/repos/lparsons/cutadapt/cutadapt/4.4+galaxy0` +- `toolshed.g2.bx.psu.edu/repos/bgruening/hifiasm/hifiasm/0.16.1+galaxy4` was updated to `toolshed.g2.bx.psu.edu/repos/bgruening/hifiasm/hifiasm/0.19.8+galaxy0` +- `toolshed.g2.bx.psu.edu/repos/iuc/busco/busco/5.3.2+galaxy0` was updated to `toolshed.g2.bx.psu.edu/repos/iuc/busco/busco/5.4.6+galaxy0` +- `toolshed.g2.bx.psu.edu/repos/iuc/merqury/merqury/1.3+galaxy2` was updated to `toolshed.g2.bx.psu.edu/repos/iuc/merqury/merqury/1.3+galaxy3` +- `toolshed.g2.bx.psu.edu/repos/iuc/datamash_ops/datamash_ops/1.1.0+galaxy2` was updated to `toolshed.g2.bx.psu.edu/repos/iuc/datamash_ops/datamash_ops/1.8+galaxy0` +- `toolshed.g2.bx.psu.edu/repos/devteam/add_value/addValue/1.0.0` was updated to `toolshed.g2.bx.psu.edu/repos/devteam/add_value/addValue/1.0.1` +- `toolshed.g2.bx.psu.edu/repos/iuc/ggplot2_point/ggplot2_point/3.4.0+galaxy0` was updated to `toolshed.g2.bx.psu.edu/repos/iuc/ggplot2_point/ggplot2_point/3.4.0+galaxy1` + + +## [0.1.1] 2023-11-20 + +- Fix author in dockstore + +## [0.1] - 2023-09-26 + +Addition of the workflow to the iwc repository. diff --git a/workflows/VGP-assembly-v2/Assembly-Hifi-only-VGP3/README.md b/workflows/VGP-assembly-v2/Assembly-Hifi-only-VGP3/README.md new file mode 100644 index 000000000..47180d7c5 --- /dev/null +++ b/workflows/VGP-assembly-v2/Assembly-Hifi-only-VGP3/README.md @@ -0,0 +1,26 @@ +## Contiging Solo w/HiC: + +Generate phased assembly based on PacBio Hifi Reads using HiC data from the same individual for phasing. + + +### Inputs + + +1. Hifi long reads [fastq] +2. HiC forward reads (if multiple input files, concatenated in same order as reverse reads) [fastq] +3. HiC reverse reads (if multiple input files, concatenated in same order as forward reads) [fastq] +4. K-mer database [meryldb] +5. Genome profile summary generated by Genomescope [txt] +6. Name of first assembly +7. Name of second assembly + + +### Outputs + +1. Haplotype 1 assembly +2. Haplotype 2 assembly +3. QC: BUSCO report for both assemblies +4. QC: Merqury report for both assemblies +5. QC: Assembly statistics for both assemblies +6. QC: Nx plot for both assemblies +7. 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`toolshed.g2.bx.psu.edu/repos/devteam/ncbi_blast_plus/ncbi_dustmasker_wrapper/2.10.1+galaxy2` was updated to `toolshed.g2.bx.psu.edu/repos/devteam/ncbi_blast_plus/ncbi_dustmasker_wrapper/2.14.1+galaxy0` +- `toolshed.g2.bx.psu.edu/repos/devteam/ncbi_blast_plus/ncbi_blastn_wrapper/2.10.1+galaxy2` was updated to `toolshed.g2.bx.psu.edu/repos/devteam/ncbi_blast_plus/ncbi_blastn_wrapper/2.14.1+galaxy0` + +## [0.1] - 2023-11-07 + +- Creation of workflow for Assembly decontamination diff --git a/workflows/VGP-assembly-v2/Assembly-decontamination-VGP9/test-data/full contaminant + mito scaffold list.txt b/workflows/VGP-assembly-v2/Assembly-decontamination-VGP9/test-data/full contaminant + mito scaffold list.txt new file mode 100644 index 000000000..50433e8b3 --- /dev/null +++ b/workflows/VGP-assembly-v2/Assembly-decontamination-VGP9/test-data/full contaminant + mito scaffold list.txt @@ -0,0 +1,2 @@ +scaffold_1058 +scaffold_1100 diff --git 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/dev/null +++ b/workflows/VGP-assembly-v2/Mitogenome-assembly-VGP0/README.MD @@ -0,0 +1,18 @@ +# Assembly of Mitochondrial DNA from PacBio HiFi reads + +Generate mitochondrial assembly based on PacBio Hifi Reads. + +## Inputs + +1. Hifi long reads [fastq] +2. Latin name of a related Species +3. Email adress required for NCBI database query + +## Outputs + +1. Contigs Statistics +2. Images : + 1. Mitogenome Coverage + 2. Mitogenome Annotation +3. Genbank file of the assembled mitogenome +4. Fasta file of the assembled mitogenome \ No newline at end of file diff --git a/workflows/VGP-assembly-v2/Purge-duplicate-contigs-VGP6/.dockstore.yml b/workflows/VGP-assembly-v2/Purge-duplicate-contigs-VGP6/.dockstore.yml new file mode 100644 index 000000000..91367ca6f --- /dev/null +++ b/workflows/VGP-assembly-v2/Purge-duplicate-contigs-VGP6/.dockstore.yml @@ -0,0 +1,10 @@ +version: 1.2 +workflows: +- name: main + subclass: Galaxy + publish: true + primaryDescriptorPath: /Purge-duplicate-contigs-VGP6.ga + authors: + - name: Galaxy + - name: VGP + url: https://vertebrategenomeproject.org diff --git a/workflows/VGP-assembly-v2/Purge-duplicate-contigs-VGP6/CHANGELOG.md b/workflows/VGP-assembly-v2/Purge-duplicate-contigs-VGP6/CHANGELOG.md new file mode 100644 index 000000000..b04c4e621 --- /dev/null +++ b/workflows/VGP-assembly-v2/Purge-duplicate-contigs-VGP6/CHANGELOG.md @@ -0,0 +1,72 @@ +# Changelog + +## [0.3.6] 2024-03-25 + +### Automatic update +- `toolshed.g2.bx.psu.edu/repos/iuc/minimap2/minimap2/2.26+galaxy0` was updated to `toolshed.g2.bx.psu.edu/repos/iuc/minimap2/minimap2/2.27+galaxy0` + +## [0.3.5] 2024-03-04 + +### Automatic update +- `toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_awk_tool/1.1.2` was updated to `toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_awk_tool/9.3+galaxy0` +- `toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_easyjoin_tool/1.1.2` was updated to `toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_easyjoin_tool/9.3+galaxy0` +- `toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_cat/0.1.1` was updated to `toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_cat/9.3+galaxy0` + +## [0.3.4] 2024-02-15 + +- Remove tag filtering +- Add merqury histogram outputs + +## [0.3.3] 2023-11-20 + +- Fix author in dockstore + +## [0.3.2] - 2023-11-15 + +### Added + +- more descriptive lables for inputs + +## [0.3.1] 2023-11-09 + +### Automatic update +- `toolshed.g2.bx.psu.edu/repos/iuc/minimap2/minimap2/2.24+galaxy0` was updated to `toolshed.g2.bx.psu.edu/repos/iuc/minimap2/minimap2/2.26+galaxy0` +- `toolshed.g2.bx.psu.edu/repos/iuc/busco/busco/5.3.2+galaxy0` was updated to `toolshed.g2.bx.psu.edu/repos/iuc/busco/busco/5.4.6+galaxy0` +- `toolshed.g2.bx.psu.edu/repos/iuc/datamash_ops/datamash_ops/1.1.0+galaxy2` was updated to `toolshed.g2.bx.psu.edu/repos/iuc/datamash_ops/datamash_ops/1.8+galaxy0` +- `toolshed.g2.bx.psu.edu/repos/iuc/merqury/merqury/1.3+galaxy2` was updated to `toolshed.g2.bx.psu.edu/repos/iuc/merqury/merqury/1.3+galaxy3` +- `toolshed.g2.bx.psu.edu/repos/devteam/add_value/addValue/1.0.0` was updated to `toolshed.g2.bx.psu.edu/repos/devteam/add_value/addValue/1.0.1` +- `toolshed.g2.bx.psu.edu/repos/iuc/ggplot2_point/ggplot2_point/3.4.0+galaxy0` was updated to `toolshed.g2.bx.psu.edu/repos/iuc/ggplot2_point/ggplot2_point/3.4.0+galaxy1` + +## [0.3] - 2023-11-08 + +### Added + +- Made BUSCO lineage selectable from workflow launch interface + +### Changed + +- Change BUSCO lineage input parameter name from `Provide lineage for BUSCO (e.g., Vertebrata)` to `Lineage` + + +## [0.2.0] - 2023-11-07 + +### Added + +- Tag filtering for inputs +- Changed parameters of gfastats for postprocessing of contigs generated with purge_dups. Specfically: + - "Terminal overlap selection" is set to "Yes" + - "Generated the initial set of paths" is set to "No" + + +## [0.1.1] - 2023-11-01 + +### Added + +- More explicit tags for readability + + +## [0.1] - 2023-10-26 + +### Added + +- Workflow for purging duplication in contigs diff --git a/workflows/VGP-assembly-v2/Purge-duplicate-contigs-VGP6/Purge-duplicate-contigs-VGP-tests.yml b/workflows/VGP-assembly-v2/Purge-duplicate-contigs-VGP6/Purge-duplicate-contigs-VGP-tests.yml new file mode 100644 index 000000000..d0439c476 --- /dev/null +++ b/workflows/VGP-assembly-v2/Purge-duplicate-contigs-VGP6/Purge-duplicate-contigs-VGP-tests.yml @@ -0,0 +1,76 @@ +- doc: Test outline for Purge-duplicate-contigs-VGP6 + job: + Hifiasm Primary assembly: + class: File + location: https://zenodo.org/records/10047837/files/Hifiasm%20Primary%20assembly.fasta?download=1 + filetype: fasta + Hifiasm Alternate assembly: + class: File + location: https://zenodo.org/records/10047837/files/Hifiasm%20Alternate%20assembly.fasta?download=1 + filetype: fasta + Meryl Database: + class: File + location: https://zenodo.org/records/10047837/files/Meryl%20Database.meryldb?download=1 + filetype: meryldb + Genomescope model parameters: + class: File + location: https://zenodo.org/records/10047837/files/Genomescope%20model%20parameters.tabular?download=1 + filetype: tabular + Estimated genome size - Parameter File: + class: File + location: https://zenodo.org/records/10047837/files/Estimated%20genome%20size%20-%20Parameter%20File.expression.json?download=1 + filetype: expression.json + Pacbio Reads Collection - Trimmed: + class: Collection + collection_type: list + elements: + - class: File + identifier: yeast_reads_sub1.fastq.gz + location: https://zenodo.org/records/10047837/files/Pacbio%20Reads%20Collection%20-%20Trimmed_yeast_reads_sub1.fastq.gz.fastq.gz?download=1 + Lineage: vertebrata_odb10 + Name of primary assembly: Primary + Name of alternate assembly: Alternate + outputs: + Cutoffs for primary assembly: + asserts: + has_text: + text: "1 15 15 16 16 48" + Purged Primary Assembly: + asserts: + has_n_lines: + n: 156 + Purged Primary Assembly (gfa): + asserts: + has_n_lines: + n: 235 + 'Busco on Purged Primary assembly: short summary': + asserts: + has_text: + text: "C:1.1%[S:1.1%,D:0.0%],F:0.5%,M:98.4%,n:3354" + Cutoffs for alternate assembly: + asserts: + has_text: + text: "1 15 15 16 16 48" + Purged Alternate assembly: + asserts: + has_n_lines: + n: 144 + Purged Alternate assembly (gfa): + asserts: + has_n_lines: + n: 217 + Assembly stats on Primary and alternate assemblies: + asserts: + has_text: + text: "# contigs 78 72" + Nx Plot: + asserts: + has_size: + value : 61000 + delta: 5000 + 'Merqury on Phased assemblies: stats': + element_tests: + output_merqury.completeness: + asserts: + has_text: + text: "both all 1212740 1300032 93.2854" diff --git a/workflows/VGP-assembly-v2/Purge-duplicate-contigs-VGP6/Purge-duplicate-contigs-VGP6.ga b/workflows/VGP-assembly-v2/Purge-duplicate-contigs-VGP6/Purge-duplicate-contigs-VGP6.ga new file mode 100644 index 000000000..d562a7dd8 --- /dev/null +++ b/workflows/VGP-assembly-v2/Purge-duplicate-contigs-VGP6/Purge-duplicate-contigs-VGP6.ga @@ -0,0 +1,3548 @@ +{ + "a_galaxy_workflow": "true", + "annotation": "Purge contigs marked as duplicates by purge_dups (could be haplotypic duplication or overlap duplication). 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It is meant to be run after one of the contigging steps (Workflow 3, 4, or 5) + +## Inputs + +1. Hifi long reads - trimmed [fastq] (Generated by Cutadapt in the contigging workflow) +2. Primary Assembly (hap1) [fasta] (Generated by the contigging workflow) +3. Alternate Assembly (hap2) [fasta] (Generated by the contigging workflow) +4. K-mer database [meryldb] (Generated by the k-mer profiling workflow) +5. Genomescope model parameters [txt] (Generated by the k-mer profiling workflow) +6. Estimated Genome Size [txt] +7. Name of first haplotype +8. Name of second haplotype +9. Lineage of you species for Busco Orthologs + +## Outputs + +1. Haplotype 1 purged assembly (Fasta and gfa) +2. Haplotype 2 purged assembly (Fasta and gfa) +3. QC: BUSCO report for both assemblies +4. QC: Merqury report for both assemblies +5. QC: Assembly statistics for both assemblies +6. QC: Nx plot for both assemblies +7. QC: Size plot for both assemblies diff --git a/workflows/VGP-assembly-v2/Purge-duplicates-one-haplotype-VGP6b/.dockstore.yml b/workflows/VGP-assembly-v2/Purge-duplicates-one-haplotype-VGP6b/.dockstore.yml new file mode 100644 index 000000000..93ba0e0cf --- /dev/null +++ b/workflows/VGP-assembly-v2/Purge-duplicates-one-haplotype-VGP6b/.dockstore.yml @@ -0,0 +1,12 @@ +version: 1.2 +workflows: +- name: main + subclass: Galaxy + publish: true + primaryDescriptorPath: /Purging-duplicates-one-haplotype-VGP6b.ga + testParameterFiles: + - /Purging-duplicates-one-haplotype-VGP6b-tests.yml + authors: + - name: Galaxy + - name: VGP + url: https://vertebrategenomeproject.org diff --git a/workflows/VGP-assembly-v2/Purge-duplicates-one-haplotype-VGP6b/CHANGELOG.md b/workflows/VGP-assembly-v2/Purge-duplicates-one-haplotype-VGP6b/CHANGELOG.md new file mode 100644 index 000000000..9c026ecd0 --- /dev/null +++ b/workflows/VGP-assembly-v2/Purge-duplicates-one-haplotype-VGP6b/CHANGELOG.md @@ -0,0 +1,22 @@ +# Changelog + +## [0.4] 2024-03-25 + +### Automatic update +- `toolshed.g2.bx.psu.edu/repos/iuc/minimap2/minimap2/2.26+galaxy0` was updated to `toolshed.g2.bx.psu.edu/repos/iuc/minimap2/minimap2/2.27+galaxy0` + +## [0.2] 2024-02-19 + +### Automatic update +- `toolshed.g2.bx.psu.edu/repos/iuc/minimap2/minimap2/2.24+galaxy0` was updated to `toolshed.g2.bx.psu.edu/repos/iuc/minimap2/minimap2/2.26+galaxy0` +- `toolshed.g2.bx.psu.edu/repos/iuc/datamash_ops/datamash_ops/1.1.0+galaxy2` was updated to `toolshed.g2.bx.psu.edu/repos/iuc/datamash_ops/datamash_ops/1.8+galaxy0` +- `toolshed.g2.bx.psu.edu/repos/iuc/merqury/merqury/1.3+galaxy2` was updated to `toolshed.g2.bx.psu.edu/repos/iuc/merqury/merqury/1.3+galaxy3` +- `toolshed.g2.bx.psu.edu/repos/iuc/busco/busco/5.3.2+galaxy0` was updated to `toolshed.g2.bx.psu.edu/repos/iuc/busco/busco/5.5.0+galaxy0` +- `toolshed.g2.bx.psu.edu/repos/devteam/add_value/addValue/1.0.0` was updated to `toolshed.g2.bx.psu.edu/repos/devteam/add_value/addValue/1.0.1` +- `toolshed.g2.bx.psu.edu/repos/iuc/ggplot2_point/ggplot2_point/3.4.0+galaxy0` was updated to `toolshed.g2.bx.psu.edu/repos/iuc/ggplot2_point/ggplot2_point/3.4.0+galaxy1` + +## [0.1] - 2024-02-07 + +### Added + +- Workflow for purging duplication in contigs in single haplotype \ No newline at end of file diff --git a/workflows/VGP-assembly-v2/Purge-duplicates-one-haplotype-VGP6b/Purging-duplicates-one-haplotype-VGP6b-tests.yml b/workflows/VGP-assembly-v2/Purge-duplicates-one-haplotype-VGP6b/Purging-duplicates-one-haplotype-VGP6b-tests.yml new file mode 100644 index 000000000..5c1a8c257 --- /dev/null +++ b/workflows/VGP-assembly-v2/Purge-duplicates-one-haplotype-VGP6b/Purging-duplicates-one-haplotype-VGP6b-tests.yml @@ -0,0 +1,76 @@ +- doc: Test outline for Purging-duplicates-one-haplotype-VGP6b + job: + Genomescope model parameters: + class: File + location: https://zenodo.org/records/10632760/files/Genomescope%20model%20parameters.tabular?download=1 + filetype: tabular + Assembly to purge: + class: File + location: https://zenodo.org/records/10632760/files/hap1.fasta?download=1 + filetype: fasta + Meryl Database: + class: File + location: https://zenodo.org/records/10632760/files/Meryl%20Database.meryldb?download=1 + filetype: meryldb + Estimated genome size - Parameter File: + class: File + location: https://zenodo.org/records/10632760/files/Estimated%20genome%20size%20-%20Parameter%20File.expression.json?download=1 + filetype: expression.json + Assembly to leave alone (need this for merqury): + class: File + location: https://zenodo.org/records/10632760/files/hap2.fasta?download=1 + filetype: fasta + Pacbio Reads Collection - Trimmed: + class: Collection + collection_type: list + elements: + - class: File + identifier: yeast_reads_sub1.fastq.gz + location: 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is the 6th workflow of the VGP pipeline. It is meant to be run after one of the contigging steps (Workflow 3, 4, or 5) + +## Inputs + +1. Genomescope model parameters [txt] (Generated by the k-mer profiling workflow) +1. Hifi long reads - trimmed [fastq] (Generated by Cutadapt in the contigging workflow) +2. Assembly to purge (e.g. hap1) [fasta] (Generated by the contigging workflow) +3. K-mer database [meryldb] (Generated by the k-mer profiling workflow) +4. Estimated Genome Size [txt] +5. Assembly to leave alone (used for merqury statistics) (e.g. hap2) [fasta] (Generated by the contigging workflow) +6. Name of un-altered assembly +7. Name of purged assembly + + +## Outputs + +1. Haplotype 1 purged assembly (Fasta and gfa) +2. Haplotype 2 purged assembly (Fasta and gfa) +3. QC: BUSCO report for both assemblies +4. QC: Merqury report for both assemblies +5. QC: Assembly statistics for both assemblies +6. QC: Nx plot for both assemblies +7. QC: Size plot for both assemblies \ No newline at end of file diff --git a/workflows/VGP-assembly-v2/Scaffolding-Bionano-VGP7/.dockstore.yml b/workflows/VGP-assembly-v2/Scaffolding-Bionano-VGP7/.dockstore.yml new file mode 100644 index 000000000..b1794de48 --- /dev/null +++ b/workflows/VGP-assembly-v2/Scaffolding-Bionano-VGP7/.dockstore.yml @@ -0,0 +1,12 @@ +version: 1.2 +workflows: +- name: main + subclass: Galaxy + publish: true + primaryDescriptorPath: /Scaffolding-BioNano-VGP7.ga + testParameterFiles: + - /Scaffolding-BioNano-VGP7-tests.yml + authors: + - name: Galaxy + - name: VGP + url: https://vertebrategenomeproject.org diff --git a/workflows/VGP-assembly-v2/Scaffolding-Bionano-VGP7/CHANGELOG.md b/workflows/VGP-assembly-v2/Scaffolding-Bionano-VGP7/CHANGELOG.md new file mode 100644 index 000000000..7c8537fc6 --- /dev/null +++ b/workflows/VGP-assembly-v2/Scaffolding-Bionano-VGP7/CHANGELOG.md @@ -0,0 +1,17 @@ +# Changelog + +## [0.1.2] 2024-03-04 + +### Automatic update +- `toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_awk_tool/1.1.2` was updated to `toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_awk_tool/9.3+galaxy0` + +## [0.1.1] 2023-11-14 + +### Automatic update +- `toolshed.g2.bx.psu.edu/repos/bgruening/bionano_scaffold/bionano_scaffold/3.7.0+galaxy0` was updated to `toolshed.g2.bx.psu.edu/repos/bgruening/bionano_scaffold/bionano_scaffold/3.7.0+galaxy3` +- `toolshed.g2.bx.psu.edu/repos/iuc/datamash_ops/datamash_ops/1.1.0+galaxy2` was updated to `toolshed.g2.bx.psu.edu/repos/iuc/datamash_ops/datamash_ops/1.8+galaxy0` +- `toolshed.g2.bx.psu.edu/repos/iuc/ggplot2_point/ggplot2_point/3.4.0+galaxy0` was updated to `toolshed.g2.bx.psu.edu/repos/iuc/ggplot2_point/ggplot2_point/3.4.0+galaxy1` + +## [0.1] - 2023-11-01 + +Addition of the workflow to the iwc repository. diff --git a/workflows/VGP-assembly-v2/Scaffolding-Bionano-VGP7/README.md b/workflows/VGP-assembly-v2/Scaffolding-Bionano-VGP7/README.md new file mode 100644 index 000000000..b5b66fca8 --- /dev/null +++ b/workflows/VGP-assembly-v2/Scaffolding-Bionano-VGP7/README.md @@ -0,0 +1,17 @@ +# Scaffolding with Bionano + +Scaffolding using Bionano optical map data + +## Inputs + +1. Bionano data [cmap] +2. Estimated genome size [txt] +3. Phased assembly generated by Hifiasm [gfa1] + +## Outputs + +1. Scaffolds +2. Non-scaffolded contigs +3. QC: Assembly statistics +4. QC: Nx plot +5. 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1.2 +workflows: +- name: main + subclass: Galaxy + publish: true + primaryDescriptorPath: /Scaffolding-HiC-VGP8.ga + testParameterFiles: + - /Scaffolding-HiC-VGP8-tests.yml + authors: + - name: VGP + url: https://vertebrategenomeproject.org + - name: Galaxy diff --git a/workflows/VGP-assembly-v2/Scaffolding-HiC-VGP8/CHANGELOG.md b/workflows/VGP-assembly-v2/Scaffolding-HiC-VGP8/CHANGELOG.md new file mode 100644 index 000000000..63ecdebac --- /dev/null +++ b/workflows/VGP-assembly-v2/Scaffolding-HiC-VGP8/CHANGELOG.md @@ -0,0 +1,44 @@ +# Changelog + +## [0.2.2] 2024-02-05 + +### Manual update + +- remove tag filtering +- set "Show grid" of Pretext Snapshot to "yes" + +### Automatic update +- `toolshed.g2.bx.psu.edu/repos/iuc/pretext_map/pretext_map/0.1.9+galaxy0` was updated to `toolshed.g2.bx.psu.edu/repos/iuc/pretext_map/pretext_map/0.1.9+galaxy1` +- `toolshed.g2.bx.psu.edu/repos/iuc/pretext_snapshot/pretext_snapshot/0.0.3+galaxy1` was updated to `toolshed.g2.bx.psu.edu/repos/iuc/pretext_snapshot/pretext_snapshot/0.0.3+galaxy2` +- `toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_awk_tool/1.1.2` was updated to `toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_awk_tool/9.3+galaxy0` +- `toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_sort_header_tool/1.1.1` was updated to `toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_sort_header_tool/9.3+galaxy0` + + +## [0.2.1] 2023-11-20 + +- Fix author in dockstore + +## [0.2] - 2023-11-15 + +### Added + +- more descriptive labels for inputs + +## [0.1.2] 2023-11-14 + +### Automatic update +- `toolshed.g2.bx.psu.edu/repos/iuc/bwa_mem2/bwa_mem2/2.2.1+galaxy0` was updated to `toolshed.g2.bx.psu.edu/repos/iuc/bwa_mem2/bwa_mem2/2.2.1+galaxy1` +- `toolshed.g2.bx.psu.edu/repos/iuc/bellerophon/bellerophon/1.0+galaxy0` was updated to `toolshed.g2.bx.psu.edu/repos/iuc/bellerophon/bellerophon/1.0+galaxy1` +- `toolshed.g2.bx.psu.edu/repos/iuc/yahs/yahs/1.2a.2+galaxy0` was updated to `toolshed.g2.bx.psu.edu/repos/iuc/yahs/yahs/1.2a.2+galaxy1` +- `toolshed.g2.bx.psu.edu/repos/iuc/busco/busco/5.3.2+galaxy0` was updated to `toolshed.g2.bx.psu.edu/repos/iuc/busco/busco/5.4.6+galaxy0` +- `toolshed.g2.bx.psu.edu/repos/iuc/datamash_ops/datamash_ops/1.1.0+galaxy2` was updated to `toolshed.g2.bx.psu.edu/repos/iuc/datamash_ops/datamash_ops/1.8+galaxy0` +- `toolshed.g2.bx.psu.edu/repos/iuc/ggplot2_point/ggplot2_point/3.4.0+galaxy0` was updated to `toolshed.g2.bx.psu.edu/repos/iuc/ggplot2_point/ggplot2_point/3.4.0+galaxy1` + +## [0.1.1] - 2023-10-26 + +Added tags for better visibility of outputs in the history, and exposure of the BUSCO lineage parameter + +## [0.1] - 2023-09-27 + +Creation of the workflow and tests + diff --git a/workflows/VGP-assembly-v2/Scaffolding-HiC-VGP8/README.md b/workflows/VGP-assembly-v2/Scaffolding-HiC-VGP8/README.md new file mode 100644 index 000000000..16413b667 --- /dev/null +++ b/workflows/VGP-assembly-v2/Scaffolding-HiC-VGP8/README.md @@ -0,0 +1,25 @@ +# Scaffolding with HiC data + +This workflow perfoms scaffolding using HiC data with YAHS. It is designed to be run as part of one the VGP analysis trajectories. +Example of trajectory : +- VGP1 : Kmer profiling +- VGP4 : Genome assembly with HiC phasing +- VGP6 : Purge duplicated haplotigs +- VGP8 : Scaffolding with HiC + +## Inputs + +1. Scaffolded assembly [fasta] +2. Concatenated HiC forward reads [fastq] +3. Concatenated HiC reverse reads [fastq] +4. Restriction enzyme sequence [txt] +5. Estimated genome size [txt] + +### Outputs + +1. Scaffolds in [fasta] and [gfa] format +2. QC: Assembly statistics +3. QC: Nx plot +4. QC: Size plot +5. QC: BUSCO report +6. 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/kmer-profiling-hifi-VGP1-tests.yml + authors: + - name: VGP + url: https://vertebrategenomeproject.org + - name: Galaxy diff --git a/workflows/VGP-assembly-v2/kmer-profiling-hifi-VGP1/.workflowhub.yml b/workflows/VGP-assembly-v2/kmer-profiling-hifi-VGP1/.workflowhub.yml new file mode 100644 index 000000000..7927f79d6 --- /dev/null +++ b/workflows/VGP-assembly-v2/kmer-profiling-hifi-VGP1/.workflowhub.yml @@ -0,0 +1,5 @@ +version: '0.1' +registries: +- url: https://workflowhub.eu + project: iwc + workflow: VGP-meryldb-creation/main diff --git a/workflows/VGP-assembly-v2/kmer-profiling-hifi-VGP1/CHANGELOG.md b/workflows/VGP-assembly-v2/kmer-profiling-hifi-VGP1/CHANGELOG.md new file mode 100644 index 000000000..261693f89 --- /dev/null +++ b/workflows/VGP-assembly-v2/kmer-profiling-hifi-VGP1/CHANGELOG.md @@ -0,0 +1,28 @@ +# Changelog + +## [0.1.5] 2023-11-20 + +- Fix author in dockstore + +## [0.1.4] 2023-11-15 + +### Added + +- more descriptive labels for inputs + +## [0.1.3] 2023-11-09 +### Automatic update +- `toolshed.g2.bx.psu.edu/repos/iuc/genomescope/genomescope/2.0+galaxy1` was updated to `toolshed.g2.bx.psu.edu/repos/iuc/genomescope/genomescope/2.0+galaxy2` + +## [0.1.2] - 2023-11-07 +### Changed +- Added tag filtering for inputs + +## [0.1.1] - 2023-10-30 +### Changed +- Names of the workflow and viles for consistency with other VGP workflows +- Labels and tags for user readability + +## [0.1] - 2021-08-26 +### Added +- First version of the workflow. diff --git a/workflows/VGP-assembly-v2/kmer-profiling-hifi-VGP1/README.md b/workflows/VGP-assembly-v2/kmer-profiling-hifi-VGP1/README.md new file mode 100644 index 000000000..5fef421b0 --- /dev/null +++ b/workflows/VGP-assembly-v2/kmer-profiling-hifi-VGP1/README.md @@ -0,0 +1,23 @@ +# VGP Workflow #1 + +This workflow produces a Meryl database and Genomescope outputs that will be used to determine parameters for following workflows, and assess the quality of genome assemblies. Specifically, it provides information about the genomic complexity, such as the genome size and levels of heterozygosity and repeat content, as well about the data quality. + +### Inputs + +- A collection of Hifi long reads in FASTQ format +- *k*-mer length +- Ploidy + +### Outputs + +- Meryl Database of kmer counts +- GenomeScope + - Linear plot + - Log plot + - Transformed linear plot + - Transformed log plot + - Summary + - Model + - Model parameteres + + ![image](https://github.com/galaxyproject/iwc/assets/4291636/565238fc-f8a9-46ac-8b31-6276410fa436) diff --git a/workflows/VGP-assembly-v2/kmer-profiling-hifi-VGP1/kmer-profiling-hifi-VGP1-tests.yml b/workflows/VGP-assembly-v2/kmer-profiling-hifi-VGP1/kmer-profiling-hifi-VGP1-tests.yml new file mode 100644 index 000000000..7c28a630e --- /dev/null +++ b/workflows/VGP-assembly-v2/kmer-profiling-hifi-VGP1/kmer-profiling-hifi-VGP1-tests.yml @@ -0,0 +1,50 @@ +- doc: Test outline for kmer-profiling-hifi-VGP1.ga + job: + Collection of 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+version: 1.2 +workflows: +- name: main + subclass: Galaxy + publish: true + primaryDescriptorPath: /kmer-profiling-hifi-trio-VGP2.ga + testParameterFiles: + - /kmer-profiling-hifi-trio-VGP2-tests.yml + authors: + - name: VGP + url: https://vertebrategenomeproject.org + - name: Galaxy diff --git a/workflows/VGP-assembly-v2/kmer-profiling-hifi-trio-VGP2/.workflowhub.yml b/workflows/VGP-assembly-v2/kmer-profiling-hifi-trio-VGP2/.workflowhub.yml new file mode 100644 index 000000000..114f0335b --- /dev/null +++ b/workflows/VGP-assembly-v2/kmer-profiling-hifi-trio-VGP2/.workflowhub.yml @@ -0,0 +1,5 @@ +version: '0.1' +registries: +- url: https://workflowhub.eu + project: iwc + workflow: VGP-meryldb-creation-trio/main diff --git a/workflows/VGP-assembly-v2/kmer-profiling-hifi-trio-VGP2/CHANGELOG.md b/workflows/VGP-assembly-v2/kmer-profiling-hifi-trio-VGP2/CHANGELOG.md new file mode 100644 index 000000000..06a53f900 --- /dev/null +++ b/workflows/VGP-assembly-v2/kmer-profiling-hifi-trio-VGP2/CHANGELOG.md @@ -0,0 +1,24 @@ +# Changelog + +## [0.1.3] 2023-11-20 + +- Fix author in dockstore + +## [0.1.2] 2023-11-09 + +### Automatic update +- `toolshed.g2.bx.psu.edu/repos/iuc/genomescope/genomescope/2.0+galaxy1` was updated to `toolshed.g2.bx.psu.edu/repos/iuc/genomescope/genomescope/2.0+galaxy2` + +## [0.1] - 2023-10-30 + +### Added +- Clearer labels + +### Removed +- Interlacing step that was unneccessary +- Requirement for paired list inputs for the Illumina parental reads. A dataset collection list is sufficient now. +- Unnecessary workflow intputs + +## [0.1] - 2021-08-26 +### Added +- First version of the workflow. diff --git a/workflows/VGP-assembly-v2/kmer-profiling-hifi-trio-VGP2/README.md b/workflows/VGP-assembly-v2/kmer-profiling-hifi-trio-VGP2/README.md new file mode 100644 index 000000000..9ba3820c4 --- /dev/null +++ b/workflows/VGP-assembly-v2/kmer-profiling-hifi-trio-VGP2/README.md @@ -0,0 +1,28 @@ +# VGP Workflow #1 + +This workflow collects the metrics on the properties of the genome under consideration by analyzing the *k*-mer frequencies. It provides information about the genomic complexity, such as the genome size and levels of heterozygosity and repeat content, as well about the data quality. It uses reads from two parental genomes to partition long reads from the offspring into haplotype-specific *k*-mer databases. + +### Inputs + +- Collection of Hifi long reads [fastq] (Collection) +- Paternal short-read Illumina sequencing reads [fastq] (Collection) +- Maternal short-read Illumina sequencing reads [fastq] (Collection) +- *k*-mer length +- Ploidy + +### Outputs + +- Meryl databases of k-mer counts + - Child + - Paternal haplotype + - Maternal haplotype +- GenomeScope metrics for child and the two parental genomes (three GenomeScope profiles in total) + - Linear plot + - Log plot + - Transformed linear plot + - Transformed log plot + - Summary + - Model + - Model parameteres + + ![image](https://github.com/galaxyproject/iwc/assets/4291636/35282f8e-d021-44f6-8e03-7b58b32d6d00) diff --git a/workflows/VGP-assembly-v2/kmer-profiling-hifi-trio-VGP2/kmer-profiling-hifi-trio-VGP2-tests.yml b/workflows/VGP-assembly-v2/kmer-profiling-hifi-trio-VGP2/kmer-profiling-hifi-trio-VGP2-tests.yml new file mode 100644 index 000000000..1d9044cfb --- /dev/null +++ b/workflows/VGP-assembly-v2/kmer-profiling-hifi-trio-VGP2/kmer-profiling-hifi-trio-VGP2-tests.yml @@ -0,0 +1,73 @@ +- doc: Test outline for kmer-profiling-hifi-trio-VGP2.ga + job: + Maternal reads: + class: Collection + collection_type: list + elements: + - class: File + identifier: forward + location: https://zenodo.org/record/6603774/files/paternal.fastq?download=1 + filetype: fastqsanger + - class: File + identifier: reverse + location: https://zenodo.org/record/6603774/files/paternal.fastq?download=1 + filetype: fastqsanger + Paternal reads: + class: Collection + collection_type: list + elements: + - class: File + identifier: forward + location: https://zenodo.org/record/6603774/files/maternal.fastq?download=1 + filetype: fastqsanger + - class: File + identifier: reverse + location: https://zenodo.org/record/6603774/files/maternal.fastq?download=1 + filetype: fastqsanger + Pacbio Hifi reads: + class: Collection + collection_type: list + elements: + - class: File + identifier: pacbio_dataset + location: https://zenodo.org/record/6603774/files/child.fastq?download=1 + filetype: fastqsanger + 'K-mer length': 9 + Ploidy: 1 + outputs: + GenomeScope linear plot (maternal): + class: File + file: test-data/GenomeScope_Linear_plot_maternal.png + compare: sim_size + delta: 15000 + GenomeScope linear plot (paternal): + class: File + file: test-data/GenomeScope_Linear_plot_paternal.png + compare: sim_size + delta: 15000 + GenomeScope linear plot (child): + class: File + file: test-data/GenomeScope_Linear_plot_child.png + compare: sim_size + delta: 15000 + GenomeScope summary (child): + asserts: + has_text: + text: '39,419 bp' + has_text: + text: '43,763 bp' + 'Meryl database : Child': + asserts: + has_size: + value: 205051 + delta: 1000 + 'Meryl database : paternal': + asserts: + has_size: + value: 40338 + delta: 1000 + 'Meryl database : maternal': + asserts: + has_size: + value: 49534 + delta: 1000 diff --git a/workflows/VGP-assembly-v2/kmer-profiling-hifi-trio-VGP2/kmer-profiling-hifi-trio-VGP2.ga b/workflows/VGP-assembly-v2/kmer-profiling-hifi-trio-VGP2/kmer-profiling-hifi-trio-VGP2.ga new file mode 100644 index 000000000..11c7afc43 --- /dev/null +++ b/workflows/VGP-assembly-v2/kmer-profiling-hifi-trio-VGP2/kmer-profiling-hifi-trio-VGP2.ga @@ -0,0 +1,1019 @@ +{ + "a_galaxy_workflow": "true", + "annotation": "Create Meryl Database used for the estimation of assembly parameters and quality control with Merqury. 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/dev/null +++ b/workflows/amplicon/dada2/README.md @@ -0,0 +1,37 @@ +# Dada2: amplicon analysis for paired end data + +## Inputs dataset + +- `Paired input data` paired input collection in FASTQ format + +## Inputs values + +- `Read length forward/reverse reads` length of the forward/reverse reads to which they should be truncated in the filter and trim step +- `Pool samples` pooling may increase sensitivity +- `Reference database` that should be used for taxonomic assignment + +## Processing + +The workflow follows the steps described in the [dada2 tutorial](https://benjjneb.github.io/dada2/tutorial.html). + +As a first step the input collection is sorted. This is important because the dada2 step outputs +a collection in sorted order. If the input collection would not be sorted then the mergePairs step +samples would be mixed up. + +- `FilterAndTrim` Quality control by filtering and trimming reads +- `QualityProfile` is called before and after the FilterAndTrim step +- `Unzip Collection` separates forward and reverse reads (the next steps are evaluated separately on forward and reverse reads) +- `learnErrors` learn error rates +- `dada` filter noisy reads +- `mergePairs` merge forward and reverse reads +- `makeSequenceTable` create the sequence table +- `removeBimeraDenovo` remove chimeric sequencs +- `assignTaxonomy` assign taxonomic information from a reference data base + +## TODO + +Some possibilities to extend/improve the workflow + +- output BIOM +- use ASV1, ... in sequence table and taxonomy output, and output additional fasta +- allow to use custom taxonomy / make it optional diff --git a/workflows/amplicon/dada2/dada2_paired-tests.yml b/workflows/amplicon/dada2/dada2_paired-tests.yml new file mode 100644 index 000000000..50d9c177d --- /dev/null +++ b/workflows/amplicon/dada2/dada2_paired-tests.yml @@ -0,0 +1,86 @@ +- doc: Test outline for dada---paired + job: + Paired input data: + class: Collection + collection_type: list:paired + elements: + - class: Collection + type: paired + identifier: F3D0 + elements: + - class: File + identifier: forward + location: https://zenodo.org/record/800651/files/F3D0_R1.fastq + - class: File + identifier: reverse + location: https://zenodo.org/record/800651/files/F3D0_R2.fastq + - class: Collection + type: paired + identifier: F3D5 + elements: + - class: File + identifier: forward + location: https://zenodo.org/record/800651/files/F3D5_R1.fastq + - class: File + identifier: reverse + location: https://zenodo.org/record/800651/files/F3D5_R2.fastq + - class: Collection + type: paired + identifier: F3D145 + elements: + - class: File + identifier: forward + location: https://zenodo.org/record/800651/files/F3D145_R1.fastq + - class: File + identifier: reverse + location: https://zenodo.org/record/800651/files/F3D145_R2.fastq + - class: Collection + type: paired + identifier: F3D150 + elements: + - class: File + identifier: forward + location: https://zenodo.org/record/800651/files/F3D150_R1.fastq + - class: File + identifier: reverse + location: https://zenodo.org/record/800651/files/F3D150_R2.fastq + - class: Collection + type: paired + identifier: Mock + elements: + - class: File + identifier: forward + location: https://zenodo.org/record/800651/files/Mock_R1.fastq + - class: File + identifier: reverse + location: https://zenodo.org/record/800651/files/Mock_R2.fastq + Read length forward read: 240 + Read length reverse read: 160 + Pool samples: 'FALSE' + Cached reference database: silva_132 + outputs: + Sequence Table: + path: test-data/Sequence Table.dada2_sequencetable + asserts: + - has_n_columns: + n: 6 + - has_n_lines: + n: 171 + Counts: + path: test-data/Counts.tabular + asserts: + - has_n_columns: + n: 8 + - has_n_lines: + n: 6 + Taxonomy: + ftype: tabular + sorted: true + asserts: + - has_text: + text: Firmicutes + n: 131 + - has_n_columns: + n: 7 + - has_n_lines: + n: 171 diff --git a/workflows/amplicon/dada2/dada2_paired.ga b/workflows/amplicon/dada2/dada2_paired.ga new file mode 100644 index 000000000..182c428f3 --- /dev/null +++ b/workflows/amplicon/dada2/dada2_paired.ga @@ -0,0 +1,824 @@ +{ + "a_galaxy_workflow": "true", + "annotation": "dada2 amplicon analysis for paired end data\n\nThe workflow has three main outputs: \n- the sequence table (output of makeSequenceTable)\n- the taxonomy (output of assignTaxonomy)\n- the counts which allow to track the number of sequences in the samples through the steps (output of sequence counts)", + "creator": [ + { + "class": "Person", + "identifier": "0000-0003-3763-0797", + "name": "Matthias Bernt" + }, + { + "class": "Organization", + "name": "UFZ Leipzig" + } + ], + "format-version": "0.1", + "license": "MIT", + "name": "dada2 amplicon analysis pipeline - for paired end data", + "release": "0.1", + "steps": { + "0": { + "annotation": "Paired input data", + "content_id": null, + "errors": null, + "id": 0, + "input_connections": {}, + "inputs": [ + { + "description": "Paired input data", + "name": "Paired input data" + } + ], + "label": "Paired input data", + "name": "Input dataset collection", + "outputs": [], + "position": { + "left": 0, + "top": 6 + }, + "tool_id": null, + "tool_state": "{\"optional\": false, \"tag\": null, \"collection_type\": \"list:paired\"}", + "tool_version": null, + "type": "data_collection_input", + "uuid": "9bccdfaa-3c84-4c13-acfe-6c41aebb0d10", + "when": null, + "workflow_outputs": [] + }, + "1": { + "annotation": "Forward reads will be truncated to this length. 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+TACGTAGGGGGCAAGCGTTATCCGGAATTACTGGGTGTAAAGGGAGCGTAGACGGTAATGCAAGTCTGGAGTGAAAGGCGGGGGCCCAACCCCCGGACTGCTCTGGAAACTGTGTAACTGGAGTGCAGGAGAGGCAGGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCCTGCTGGACTGTAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGG 280 11 0 38 0 +TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTAGGCGGTTGGTCGCGTCTGCCGTGAAATCCTCTGGCTTAACTGGGGGCGTGCGGTGGGTACGGGCTGACTTGAGTGCGGTAGGGGAGACTGGAACTCCTGGTGTAGCGGTGGAATGCGCAGATATCAGGAAGAACACCGGTGGCGAAGGCGGGTCTCTGGGCCGTTACTGACGCTGAGGAGCGAAAGCGTGGGGAGCGAACAGG 0 0 0 0 325 +TACGTAGGTGGCAAGCGTTGTCCGGATTTACTGGGCGTAAAGGGAGCGTAGGTGGATATTTAAGTGGGATGTGAAATACTCGGGCTTAACCTGGGTGCTGCATTCCAAACTGGATATCTAGAGTGCAGGAGAGGAAAGTAGAATTCCTAGTGTAGCGGTGAAATGCGTAGAGATTAGGAAGAATACCAGTGGCGAAGGCGACTTTCTGGACTGTAACTGACACTGAGGCTCGAAAGCGTGGGGAGCAAACAGG 0 5 0 0 300 +TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGGGCGCAGACGGTTACTTAAGCAGGATGTGAAATCCCCGGGCTCAACCCGGGAACTGCGTTCTGAACTGGGTGACTCGAGTGTGTCAGAGGGAGGTAGAATTCCACGTGTAGCAGTGAAATGCGTAGAGATGTGGAGGAATACCGATGGCGAAGGCAGCCTCCTGGGACAACACTGACGTTCATGCCCGAAAGCGTGGGTAGCAAACAGG 0 4 0 0 289 +TACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGAGCGCGCAGGTGGTTAATTAAGTCTGATGTGAAAGCCCACGGCTTAACCGTGGAGGGTCATTGGAAACTGGTTGACTTGAGTGCAGAAGAGGGAAGTGGAATTCCATGTGTAGCGGTGAAATGCGTAGAGATATGGAGGAACACCAGTGGCGAAGGCGGCTTCCTGGTCTGCAACTGACACTGAGGCGCGAAAGCGTGGGGAGCAAACAGG 80 126 30 36 0 +TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTCCGTTAAGTCAGCGGTAAAATTGCGGGGCTCAACCCCGTCGAGCCGTTGAAACTGGCAGACTTGAGTTGGCGAGAAGTACGCGGAATGCGCGGTGTAGCGGTGAAATGCATAGATATCGCGCAGAACTCCGATTGCGAAGGCAGCGTACCGGCGCCAGACTGACGCTGAGGCACGAAAGCGTGGGGATCGAACAGG 69 105 49 37 0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAGGCGGAATTCGTGGTGTAGCGGTGAAATGCTTAGATATCACGAAGAACTCCGATTGCGAAGGCAGCCTGCTAAGCTGCAACTGACATTGAGGCTCGAAAGTGTGGGTATCAAACAGG 0 0 0 0 216 +TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCAGCGCAAGTCTGGAGTGAAATGCCGGGGCCCAACCCCGGAACTGCTTTGGAAACTGTGCAGCTCGAGTGCAGGAGAGGTAAGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACTGTAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGG 93 15 19 87 0 +TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGGGAGCGCAGGCGGTCAGGAAAGTCTGGAGTAAAAGGCTATGGCTCAACCATAGTGTGCTCTGGAAACTGTCTGACTTGAGTGCAGAAGGGGAGAGTGGAATTCCATGTGTAGCGGTGAAATGCGTAGATATATGGAGGAACACCAGTGGCGAAAGCGGCTCTCTGGTCTGTCACTGACGCTGAGGCTCGAAAGCGTGGGTAGCGAACAGG 0 0 0 0 211 +TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGACAGTTAAGTCAGCGGTAAAATTGAGAGGCTCAACCTCTTCCCGCCGTTGAAACTGATTGTCTTGAGTGGGCGAGAAGTATGCGGAATGCGTGGTGTAGCGGTGAAATGCATAGATATCACGCAGAACTCCGATTGCGAAGGCAGCATACCGGCGCCCAACTGACGCTGAAGCACGAAAGCGTGGGTATCGAACAGG 65 121 0 20 0 +TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTGAGACAAGTCTGAAGTGAAAGCCCGGGGCTCAACCCCGGGACTGCTTTGGAAACTGCCTGACTGGAGTGCTGGAGAGGCAAGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAAGAACACCAGTGGCGAAGGCGGCTTGCTGGACAGTAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGG 44 13 140 0 0 +TACGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGGGAACGCAGGCGGTCTTTTAAGTCTGATGTGAAAGCCTTCGGCTTAACCGGAGTAGTGCATTGGAAACTGGGAGACTTGAGTGCAGAAGAGGAGAGTGGAACTCCATGTGTAGCGGTGAAATGCGTAGATATATGGAAGAACACCAGTGGCGAAAGCGGCTCTCTGGTCTGTAACTGACGCTGAGGTTCGAAAGCGTGGGTAGCAAACAGG 52 22 64 48 0 +TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAGGGGGGTAGAATTCCAGGTGTAGCGGTGAAATGCGTAGAGATCTGGAGGAATACCGGTGGCGAAGGCGGCCCCCTGGACGAAGACTGACGCTCAGGTGCGAAAGCGTGGGGAGCAAACAGG 0 0 0 4 179 +TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCAGGCGGTTTCTTAAGTCTGATGTGAAAGCCCCCGGCTCAACCGGGGAGGGTCATTGGAAACTGGGAGACTTGAGTGCAGAAGAGGAGAGTGGAATTCCATGTGTAGCGGTGAAATGCGTAGATATATGGAGGAACACCAGTGGCGAAGGCGGCTCTCTGGTCTGTAACTGACGCTGAGGCTCGAAAGCGTGGGGAGCAAACAGG 0 0 0 0 181 +TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCAGTGCAAGTCTGAAGTGAAAGGCGGGGGCCCAACCCCCGGACTGCTTTGGAAACTGTGCTGCTGGAGTGCAGGAGAGGCAAGTGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTGCTGGACTGTAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGG 74 13 31 43 0 +TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCAGGCGGTTCTTTAAGTCTGAAGTTAAAGGCAGTGGCTTAACCATTGTACGCTTTGGAAACTGGAGGACTTGAGTGCAGAAGGGGAGAGTGGAATTCCATGTGTAGCGGTGAAATGCGTAGATATATGGAGGAACACCGGTGGCGAAAGCGGCTCTCTGGTCTGTAACTGACGCTGAGGCTCGAAAGCGTGGGGAGCAAACAGG 0 0 0 0 157 +TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGCGCGCAGGCGGTCTTTTAAGTCTGATGTGAAAGCCCCCGGCTTAACCGGGGAGGGTCATTGGAAACTGGAAGACTGGAGTGCAGAAGAGGAGAGTGGAATTCCACGTGTAGCGGTGAAATGCGTAGATATGTGGAGGAACACCAGTGGCGAAGGCGACTCTCTGGTCTGTAACTGACGCTGAGGCGCGAAAGCGTGGGGAGCAAACAGG 0 0 0 0 150 +TACGTAGGTGGCGAGCGTTGTCCGGATTTACTGGGCGTAAAGGGAGCGTAGGCGGACTTTTAAGTGAGATGTGAAATACTCGGGCTCAACTTGAGTGCTGCATTTCAAACTGGAAGTCTAGAGTGCAGGAGAGGAGAATGGAATTCCTAGTGTAGCGGTGAAATGCGTAGAGATTAGGAAGAACACCAGTGGCGAAGGCGATTCTCTGGACTGTAACTGACGCTGAGGCTCGAAAGCGTGGGGAGCAAACAGG 128 14 6 0 0 +TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCCAGACAAGTCTGAAGTGAAAATCCAGCGCTTAACGTTGGAAGTGCTTTGGAAACTGCCGGGCTAGAGTGCAGGAGGGGCAGGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCCTGCTGGACTGCAACTGACGTTGAGGCTCGAAGGCGTGGGGAGCAAACAGG 62 0 0 78 0 +TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTGCGCCAAGTCTGGAGTGAAATGCCGCAGCTTAACTGCGGAACTGCTTTGGAAACTGGCGAACTAGAGTGCGGGAGGGGTAAGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCGGTGGCGAAGGCGGCTTACTGGACCGTAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGG 54 8 23 55 0 +TACGAAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTAGGTGGTTCAGCAAGTTGGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCCAAAACTACTGAGCTAGAGTACGGTAGAGGGTGGTGGAATTTCCTGTGTAGCGGTGAAATGCGTAGATATAGGAAGGAACACCAGTGGCGAAGGCGACCACCTGGACTGATACTGACACTGAGGTGCGAAAGCGTGGGGAGCAAACAGG 0 0 0 0 140 +TACGTAGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGTGCAGCCGGAGAGACAAGTCAGATGTGAAATCCACGGGCTCAACCCGTGAACTGCATTTGAAACTGTTTCCCTTGAGTGTCGGAGAGGTAATCGGAATTCCTTGTGTAGCGGTGAAATGCGTAGATATAAGGAAGAACACCAGTGGCGAAGGCGGATTACTGGACGATAACTGACGGTGAGGCGCGAAAGCGTGGGGAGCAAACAGG 75 6 6 52 0 +TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTTTTGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGGACTGCATTGGAAACTGTATGACTGGAGTGCAGGAGAGGTAAGTGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACTGTAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGG 70 0 6 57 0 +TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGGGCGCAGACGGCCATGGCAAGCCCGGTGTGAAAGGCAGGGGCATAACCCCTGGACTGCACTGGGAACTGTCAGGCTGGAGTGCCGGAGGGGTAAGCGGAATTCCTGGTGTAGCGGTGAAATGCGTAGATATCAGGAGGAACACCGGCGGCGAAGGCGGCTTACTGGACGGCAACTGACGTTGAGGCCCGAAAGCGTGGGGAGCGAACAGG 28 18 85 0 0 +TACGTAGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGTGCAGCCGGGAAGACAAGTCAGATGTGAAATCCCGCGGCTCAACCGCGGAACTGCATTTGAAACTGTTTTTCTTGAGTACCGGAGAGGTCATCGGAATTCCTTGTGTAGCGGTGAAATGCGTAGATATAAGGAAGAACACCAGTGGCGAAGGCGGATGACTGGACGGCAACTGACGGTGAGGCGCGAAAGCGTGGGGAGCAAACAGG 31 18 32 49 0 +TACGTAGGGGGCGAGCGTTGTCCGGAATCACTGGGCGTAAAGGGCGCGTAGGCGGTTTAATAAGTCAGTGGTGAAAACTGAGGGCTCAACCCTCAGCCTGCCACTGATACTGTTAGACTTGAGTATGGAAGAGGAGAATGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGATTCTCTGGGCCAAGACTGACGCTGAGGCGCGAAAGCGTGGGGAGCAAACAGG 26 20 54 25 0 +TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCGATGCAAGTCTGAAGTGAAAGGCGGGGGCCCAACCCCCGGACTGCTTTGGAAACTGTATAGCTGGAGTGCAGGAGAGGTAAGTGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACTGTAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGG 58 17 25 21 0 +TACGGAGGGTGCAAGCGTTACCCGGAATCACTGGGCGTAAAGGGCGTGTAGGCGGAAATTTAAGTCTGGTTTTAAAGACCGGGGCTCAACCTCGGGGATGGACTGGATACTGGATTTCTTGACCTCTGGAGAGGTAACTGGAATTCCTGGTGTAGCGGTGGAATGCGTAGATACCAGGAGGAACACCAATGGCGAAGGCAAGTTACTGGACAGAAGGTGACGCTGAGGCGCGAAAGTGTGGGGAGCAAACCGG 0 2 0 0 112 +TACGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCAGGCGGTTGCTTAGGTCTGATGTGAAAGCCTTCGGCTTAACCGAAGAAGTGCATCGGAAACCGGGCGACTTGAGTGCAGAAGAGGACAGTGGAACTCCATGTGTAGCGGTGGAATGCGTAGATATATGGAAGAACACCAGTGGCGAAGGCGGCTGTCTGGTCTGCAACTGACGCTGAGGCTCGAAAGCATGGGTAGCGAACAGG 42 39 19 10 0 +TACGTAGGGGGCGAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGCGTGGCAAGTCAGATGTGAAAACCCGGGGCCCAACCCCGGGACTGCATTTGAAACTGCCATGCTGGAGTGCCGGAGAGGCAAGTGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGGAGGCGGCTTGCTGGACGGTAACTGACGCTGAGGCTCGAAAGCGTGGGGAGCAAACAGG 55 0 0 54 0 +TACGTAGGGGGCAAGCGTTATCCGGAATTACTGGGTGTAAAGGGAGCGTAGACGGTGATGCAAGTCTGGAGTGAAAGGCGGGGGCCCAACCCCCGGACTGCTCTGGAAACTGTGTAACTTGAGTGCAGGAGAGGTAAGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACTGTAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGG 6 13 53 33 0 +TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCAATGCAAGCCAGATGTGAAAACCCGCAGCTCAACTGGGGGAGTGCATTTGGAACTGTGTAGCTGGAGTGCAGGAGAGGTAAGCGGAATTCCTGGTGTAGCGGTGAAATGCGTAGATATCAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACTGTAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGG 43 11 0 45 0 +TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGTTGTTCGGTAAGTCAGCGGTGAAACCTGAGCGCTCAACGTTCAGCCTGCCGTTGAAACTGCCGGGCTTGAGTTCAGCGGCGGCAGGCGGAATTCGTGGTGTAGCGGTGAAATGCATAGATATCACGAGGAACTCCGATTGCGAAGGCAGCTTGCCATACTGCGACTGACACTGAAGCACGAAGGCGTGGGTATCAAACAGG 0 0 0 0 97 +TACGTAGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGTGTAGCCGGGCTTACAAGTCAGATGTGAAATCCGGGGGCTCAACCCCCGAACTGCATTTGAAACTGTAGGTCTTGAGTATCGGAGAGGCAGGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCCTGCTGGACGACAACTGACGGTGAGGCGCGAAAGCGTGGGGAGCAAACAGG 62 0 0 34 0 +TACGTAGGGAGCGAGCGTTATCCGGATTTATTGGGTGTAAAGGGTGCGTAGGCGGTAATACAGGTCTTTGGTATAAGCCCGAAGCTTAACTTCGGTAAGCCAGAGAAACCGTATAACTAGAGTATGGTAGAGGCAAGTGGAATTTCTAGTGTAGCGGTAAAATGCGTAGATATTAGAAGGAACACCAGTGGCGAAGGCGACTTGCTGGGCCAAAACTGACGCTGAGGCACGAAAGCGTGGGGAGCAAATAGG 67 5 5 17 0 +TACGTAGGGAGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCGTAGGCGGCCTTGCAAGTCAGAAGTGAAATCCATGGGCTTAACCCGTGAACTGCTTTTGAAACTGTAGGGCTTGAGTGAAGTAGAGGCAGGCGGAATTCCCGGTGTAGCGGTGAAATGCGTAGAGATCGGGAGGAACACCAGTGGCGAAGGCGGCCTGCTGGGCTTTAACTGACGCTGAAGCACGAAAGCGTGGGTAGCAAACAGG 50 11 13 20 0 +TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGACGGCTGTGCAAGTCTGGAGTGAAAGGCGGGGGCCCAACCCCCGGACTGCTCTGGAAACTGTAAAGCTGGAGTGCAGGAGAGGTAAGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACTGCAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGG 45 0 0 48 0 +TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTGATGCAAGTCTGAAGTGAAAGGCGGGGGCTCAACCCCCGGACTGCTTTGGAAACTGTATGACTGGAGTGCAGGAGAGGTAAGTGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACTGTAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGG 92 0 0 0 0 +TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTGCGCCAAGTCTGGAGTGAAATGCCGCAGCTTAACTGCGGAACTGCTTTGGAAACTGGCGGACTAGAGTGCGGGAGGGGTAAGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCGGTGGCGAAGGCGGCTTACTGGACCGTAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGG 57 0 0 35 0 +TACGTAGGGAGCGAGCGTTATCCGGAATTATTGGGCGTAAAGGGTGCGTAGATGGCATAGTAAGTCTTTTGTAAAAATGCTGGGCTCAACCCAGTAGGGCAAAAGATACTGCAAAGCTAGAGTATGACAGAGGCAAGTGGAACTACATGTGTAGCGGTAAAATGCGTAAATATATGTAAGAACACCAGTGGCGAAGGCGGCTTGCTGGGTCGATACTGACATTGAGGCACGAAAGCGTGGGGAGCAAACAGG 84 3 0 0 0 +TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCTGTGCAAGTCTGAAGTGAAAGCCCGTGGCTCAACCGCGGAACTGCTTTGGAAACTGTATAGCTTGAGTACTGGAGAGGCAGGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCCTGCTGGACAGAAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGG 27 14 20 23 0 +TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGACGGCAGTGCAAGTCTGGAGTGAAAGCCCGGGGCCCAACCCCGGAACTGCTCTGGAAACTGTGCGGCTAGAGTACTGGAGGGGCAGGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCCTGCTGGACAGTAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGG 18 6 41 18 0 +TACGTAGGGGGCGAGCGTTATCCGGATTCACTGGGCGTAAAGGGAGCGCAGGCGGCACGGCAAGTCCGGTGTGAAAGCCCGGGGCCCAACCCCGGGACTGCACTGGAAACTGCCGGGCTGGAGTGCCGGAGGGGTAAGCGGAATTCCTGGTGTAGCGGTGAAATGCGCAGATATCAGGAGGAACACCAGTGGCGGAGGCGGCTTACTGGACGGTCACTGACGCTGAGGCTCGAAAGCGTGGGGAGCAAACAGG 50 0 25 7 0 +TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCATAGCAAGTCTGAAGTGAAAGCCCAAGGCTCAACCATGGGACTGCTTTGGAAACTGTTAAGCTAGAGTGCTGGAGAGGTAAGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAAGAACACCAGTGGCGAAGGCGGCTTACTGGACAGTAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGG 5 0 16 52 0 +TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGCATGGCAAGTCAGATGTGAAAGCCCGGGGCTCAACCCCGGGACTGCATTTGAAACTGCCAGGCTAGAGTGTCGGAGAGGCAAGTGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTGCTGGACGATCACTGACGCTGAGGCTCGAAAGCGTGGGGAGCAAACAGG 32 0 0 34 0 +TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCGCGGCAAGTCTGAAGTGAAAGGCAGGGGCTTAACCCCTGAACTGCTTTGGAAACTGCCATGCTAGAGTGCTGGAGAGGTAAGTGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACAGTAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGG 27 0 29 10 0 +TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTCCTGCAAGCCAGATGTGAAAGCCCGGGGCTCAACCCCGGGACTGCATTTGGAACTGTAAGGCTAGAGTGTCGGAGAGGCAGGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCCTGCTGGACGATGACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGG 47 0 0 18 0 +TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTGTCAAGTCAGCGGTAAAAATTCCGGGCTCAACCCGGTCCCGCCGTTGAAACTGGCGAACTCGAGAGGGAGAGAAGTAGGCGGAATGCGCAGTGTAGCGGTGAAATGCATAGATATTGCGCAGAACTCCGATTGCGAAGGCAGCCTACCGGCTCCTATCTGACGCTGAGGCACGAAAGCGTGGGTATCGAACAGG 57 0 0 0 0 +TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCGAAGAAAGTCTGAAGTGAAAGCCCGCGGCTCAACCGCGGAATGGCTTTGGAAACTTTTTTGCTGGAGTACCGGAGAGGTAAGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACGGTAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGG 6 13 27 10 0 +TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCCAGACAAGTCTGAAGTGAAAATCCAGCGCTTAACGTTGGAAGTGCTTTGGAAACTGCCGGGCTGGAGTGCAGGAGGGGCAGGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCCTGCTGGACTGCAACTGACGTTGAGGCTCGAAGGCGTGGGGAGCAAACAGG 0 0 0 55 0 +TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCAGCGCAAGTCTGAAGTGAAATGCCAGGGCTTAACCCTGGAACTGCTTTGGAAACTGTGCAGCTAGAGTGCAGGAGAGGTAAGTGGAATTTCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACTGTAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGG 0 13 41 0 0 +TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTTATGTAAGTCTGGAGTGAAAGCCCGGGGCCCAACCCCGGGACTGCTTTGGAAACTGTGTAACTGGAGTACAGGAGGGGCAGGCGGAATTCCTGGTGTAGCGGTGAAATGCGTAGATATCAGGAGGAACACCGGCGGCGAAGGCGGCCTGCTGGACTGAAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGG 42 0 0 11 0 +TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTTGTGCAAGTCTGAAGTGAAAGCCCGCGGCTCAACTGCGGAACGGCTTTGGAAACTGTGTAACTGGAGTGCTGGAGAGGTAAGCGGAATTCCTGGTGTAGCGGTGAAATGCGTAGAGATCAGGAGGAACACCGGCGGCGAAGGCGGCTTACTGGACAGTAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGG 19 5 23 6 0 +TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCAGAGCAAGTCTGAAGTGAAAGCCCGCGGCCCAACTGCGGGACTGCTTTGGAAACTGCCCGGCTAGAGTGCTGGAGAGGTAAGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACAGTAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGG 18 0 15 19 0 +TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCTGTGCAAGTCTGAAGTGAAAGCCCAGGGCTCAACCCTGGGACTGCTTTGGAAACTGTGGAGCTAGAGTGCTGGAGAGGTAAGTGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACAGTAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGG 0 0 52 0 0 +TACGGAGGGGGCTAGCGTTATTCGGAATTACTGGGCGTAAAGCGCACGTAGGCGGATCGGAAAGTCAGAGGTGAAATCCCAGGGCTCAACCCTGGAACTGCCTTTGAAACTCCCGATCTTGAGGTCGAGAGAGGTGAGTGGAATTCCGAGTGTAGAGGTGAAATTCGTAGATATTCGGAGGAACACCAGTGGCGAAGGCGGCTCACTGGCTCGATACTGACGCTGAGGTGCGAAAGCGTGGGGAGCAAACAGG 0 0 0 0 52 +TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGGCGGCCATGCAAGTCAGAAGTGAAAACCCGGGGCTCAACCCTGGGAGTGCTTTTGAAACTGTGCGGCTAGAGTGTCGGAGGGGTAAGTGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACGATGACTGACGCTGAGGCTCGAAAGCGTGGGGAGCAAACAGG 34 0 0 16 0 +TACGTAGGGTGCAAGCGTTATCCGGATTTATTGGGCGTAAAGGGCTCGTAGGCGGTTCGTCGCGTCCGGTGTGAAAGTCCATCGCTTAACGGTGGATCCGCGCCGGGTACGGGCGGGCTTGAGTGCGGTAGGGGAGACTGGAATTCCCGGTGTAACGGTGGAATGTGTAGATATCGGGAAGAACACCAATGGCGAAGGCAGGTCTCTGGGCCGTTACTGACGCTGAGGAGCGAAAGCGTGGGGAGCGAACAGG 24 7 19 0 0 +TACGTAGGGGGCAAGCGTTATCCGGAATCACTGGGTGTAAAGGGAGCGTAGACGGCTGTGCAAGCCTGAAGTGAAAGGCGGGGGCCCAACCCCTGGACTGCTTTGGGAACTGTACGGCTGGAGTGCAGGAGAGGTAAGTGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACTGTAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGG 47 0 0 0 0 +TACGTAGGGGGCGAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTTCTGCAAGTCTGAAGTGAAAGCCCGTGGCTTAACCGCGGAACGGCTTTGGAAACTGTGGAACTGGAGTGCTGGAGAGGCAAGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTGCTGGACAGTAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGG 0 0 47 0 0 +TACGTAGGGGGCAAGCGTTATCCGGATTCACTGGGTGTAAAGGGAGCGTAGACGGCCATGCAAGCCAGGGGTGAAAGCCCGGGGCCCAACCCCGGGACTGCCCTTGGAACTGTGCGGCTGGAGTGCGGGAGGGGCAGGCGGAATTCCTGGTGTAGCGGTGAAATGCGTAGATATCAGGAGGAACACCGGCGGCGAAGGCGGCCTGCTGGACCGCGACTGACGTTGAGGCTCGAAAGCGTGGGGAGCGAACAGG 20 0 0 25 0 +TACGTAGGGAGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCGTAGGCGGCTTTGCAAGTCAGAAGTGAAATCCATGGGCTTAACCCATGAACTGCTTTTGAAACTGCAGAGCTTGAGTGGAGTAGAGGTAGGCGGAATTCCCGGTGTAGCGGTGAAATGCGTAGAGATCGGGAGGAACACCAGTGGCGAAGGCGGCCTGCTGGGCTCTAACTGACGCTGAGGCACGAAAGCGTGGGTAGCAAACAGG 35 0 0 9 0 +TACGTATGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGGCGGCCTGGCAAGCCTGATGTGAAATACCGGGGCCCAACCCCGGGGCTGCATTGGGAACTGCCAGGCTGGAGTGCCGGAGAGGCAGGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCCTGCTGGACGGTGACTGACGCTGAGGCTCGAAAGCGTGGGGAGCAAACAGG 0 44 0 0 0 +TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCTGTGCAAGTCTGAAGTGAAATGCCGGGGCCCAACCCCGGAACTGCTTTGGAAACTGTACAGCTAGAGTGCAGGAGGGGTGAGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCGGTGGCGAAGGCGGCTCACTGGACTGTAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGG 0 4 14 26 0 +TACGTAGGGAGCAAGCGTTGTTCGGAATGACTGGGCGTAAAGGGTGCGTAGGCGGTTGTACAAGTTAGAAGTGAAATACCCAGGGCTTAACTCGGGTGCTGCTTCTAAAACTGTATGACTTGAGTGCAGTAGAGGTTAGTGGAATTCCTAGTGTAGCGGTGGAATGCGTAGATATTAGGAGGAACACCAGAGGCGAAGGCGGCTAACTGGACTGCAACTGACGCTGAGGCACGAAAGTGTGGGGAGCAAACAGG 26 6 0 11 0 +TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCGATGCAAGTCTGAAGTGAAAGCCCGGGGCTCAACCCCGGGACTGCTTTGGAAACTGTATGGCTGGAGTGCTGGAGAGGCAAGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAAGAACACCAGTGGCGAAGGCGGCTTGCTGGACAGTAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGG 10 0 0 33 0 +TACGTAGGGGGCAAGCGTTATCCGGATTCACTGGGCGTAAAGGGAGCGCAGGCGGCACGGCAAGTCTGGTGTGAAAACCCGGGGCTCAACCCCGGGACTGCATTGGAAACTGCCGGGCTGGAGTGTCGGAGGGGTAAGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACGATGACTGACGCTGAGGCTCGAAAGCGTGGGGAGCAAACAGG 41 0 0 0 0 +TACGTAGGTGGCAAGCGTTATCCGGATTTATTGGGTGTAAAGGGCGTGTAGGCGGGAATGCAAGTCAGATGTGAAAACTATGGGCTCAACCCATAGCCTGCATTTGAAACTGTATTTCTTGAGTGCTGGAGAGGCAATCGGAATTCCGTGTGTAGCGGTGAAATGCGTAGATATACGGAGGAACACCAGTGGCGAAGGCGGATTGCTGGACAGTAACTGACGCTGAGGCGCGAAAGCGTGGGGAGCAAACAGG 0 10 12 19 0 +TACGTAGGGGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGAGTACGTAGGTGGCAACCTAAGCGCAGGGTTTAAGGCAATGGCTCAACCATTGTTCGCCCTGCGAACTGGGATGCTTGAGTGCAGGAGAGGAAAGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTTCTGGACTGTAACTGACACTGAGGTACGAAAGCGTGGGGAGCAAACAGG 5 21 8 6 0 +TACGTAGGTGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGTGTAGGCGGGAAAGCAAGTCAGATGTGAAAACCATGGGCTCAACCTGTGGCCTGCATTTGAAACTGTTTTTCTTGAGTACTGGAGAGGCAGACGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGTCTGCTGGACAGCAACTGACGCTGAGGCGCGAAAGCGTGGGGAGCAAACAGG 17 9 0 13 0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATATTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAGGCGGAATTCGTGGTGTAGCGGTGAAATGCTTAGATATCACGAAGAACTCCGATTGCGAAGGCAGCCTGCTAAGCTGCAACTGACATTGAGGCTCGAAAGTGTGGGTATCAAACAGG 0 0 0 0 38 +TACGTAGGGGGCAAGCGTTATCCGGAATTACTGGGTGTAAAGGGAGCGTAGACGGTGATGTAAGTCTGGAGTGAAAGGCGGGGGCCCAACCCCCGGACTGCTCTGGAAACTATGTGACTGGAGTGCAGGAGAGGTGAGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTCACTGGACTGTAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGG 14 0 0 23 0 +TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGCGCGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGGACTGCATTGGAAACTGTCGCGCTCGAGTGTCGGAGAGGTAAGTGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACGACAACTGACGCTGAGGCTCGAAAGCGTGGGGAGCAAACAGG 24 0 0 12 0 +TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCAGGGCAAGTCTGGAGTGAAAGGCAGGGGCCCAACCCCTGGACTGCTCTGGAAACTGTCCGGCTGGAGTGCAGGAGAGGTAAGTGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACTGTAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGG 13 0 0 22 0 +TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTACGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGCCGGACTGGAGTGTCGGAGGGGTAAGCGGAATTCCTGGTGTAGCGGTGAAATGCGTAGATATCAGGAGGAACACCGGTGGCGAAGGCGGCTTACTGGACGATGACTGACGCTGAGGCTCGAAAGCGTGGGGAGCAAACAGG 34 0 0 0 0 +TACGTAGGGGGCGAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCGTCGCAAGTCTGAAGTGAAAGCCCGTGGCTCAACCGCGGAACCGCTTTGGAAACTGCGAGGCTGGAGTGCTGGAGAGGTAAGCGGAATTCCTGGTGTAGCGGTGAAATGCGTAGATATCAGGAGGAACACCGGTGGCGAAGGCGGCTTACTGGACAGTGACTGACGTTGAGGCTCGAAAGCGTGGGGAGCGAACAGG 8 0 20 6 0 +TACGTAGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGTGTAGCCGGGTTGACAAGTCAGATGTGAAATCCTGCGGCTTAACCGCAGAACTGCATTTGAAACTGTTGATCTTGAGTACTGGAGAGGCAGACGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGTCTGCTGGACAGCAACTGACGGTGAGGCGCGAAAGCGTGGGGAGCAAACAGG 0 9 12 13 0 +TACGTAGGTGGCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGTCCGCAGCCGGTTTATTAAGTATAGAATAAAACTTCGGGGCTCAACCCCGTCTCGTTCTATAAACTGATAGACTAGAGTGTGGTAGAGGCAAGTGGAATTTCTAGTGTAGCGGTAGAATGCGTAGATATTAGAAGGAACACCAGTGGCGAAGGCGACTTGCTGGGCCATCACTGACGGTCAGGGACGAAAGCGTGGGGAGCGAATAGG 0 18 14 0 0 +TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGACTTGCAAGTCTGATGTGAAAATCCGGGGCCCAACCCCGGAACTGCATTGGAAACTGTATATCTAGAGTGTCGGAGAGGCAAGTGGAATTCCTGGTGTAGCGGTGAAATGCGTAGATATCAGGAGGAACACCAGTGGCGAAGGCGGCTTGCTGGACGATGACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGG 0 14 12 6 0 +TACGTAGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGTGTAGCCGGGCTGACAAGTCAGATGTGAAATCCGGGGGCTCAACCCCCGAACTGCATTTGAAACTGTTGGTCTTGAGTATCGGAGAGGCAGGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCCTGCTGGACGACAACTGACGGTGAGGCGCGAAAGCGTGGGGAGCAAACAGG 0 0 31 0 0 +TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGGCGGTTTAGCAAGTCAGAAGTGAAAGCCCGGGGCTCAACTCCGGGACTGCTTTTGAAACTGTTGAACTAGATTGCAGGAGAGGTAAGTGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACTGTAAATGACGCTGAGGCTCGAAAGCGTGGGGAGCAAACAGG 19 0 0 9 0 +TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGGGCGCAGACGGCAGCGCAAGCCAGGAGTGAAAGCCCGGGGCCCAACCCCGGGACTGCTCTTGGAACTGCGCGGCTGGAGTGCAGGAGGGGCAGGCGGAATTCCTGGTGTAGCGGTGAAATGCGTAGATATCAGGAGGAACACCGGTGGCGAAGGCGGCCTGCTGGACTGCGACTGACGTTGAGGCCCGAAAGCGTGGGGAGCAAACAGG 5 0 11 12 0 +TACGTAGGGAGCGAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGTAGGCGGGCGAGAAAGTTGAATGTTAAATCTACCGGCTTAACTGGTAGCTGCGTTCAAAACTTCTTGTCTTGAGTGAAGTAGAGGCAGGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCCTGCTGGGCTTTAACTGACGCTGAGGCTCGAAAGCGTGGGGAGCAAACAGG 0 11 17 0 0 +TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCGGTGCAAGCCAGATGTGAAAGCCCGGGGCTCAACCCCGGGACTGCATTTGGAACTGTGCTGCTAGAGTGTCGGAGAGGCAGGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCCTGCTGGACGATGACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGG 0 0 14 13 0 +TACGTATGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGGCGGCAGGGCAAGCCTGATGTGAAAGCCCGGGGCTCAACCCCGGGACTGCATTGGGAACTGCCCCGCTCGAGTGCCGGAGGGGCAGGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCCTGCTGGACGGTGTACTGACGCTGAGGCTCGAAAGCGTGGGGAGCAAACAGG 16 0 10 0 0 +TACGTAGGAGGCAAGCGTTATCCGGAATGACTGGGCGTAAAGGGTGCGTAGGTGGTTTGACAAGTTGGTAGCGTAACTCCGGGGCTCAACCTCGGCACTACTACCAAAACTGTTGGACTTGAGTGCAGGAGGGGCAAATGGAATTCCTAGTGTAGCGGTGGAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGATTTGCTGGACTGTAACTGACACTGAGGCACGAAAGCGTGGGGAGCAAACAGG 0 10 10 6 0 +TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGGTGGCATGGCAAGCCAGAAGTGAAAACCCGGGGCTTAACCCCGCGGATTGCTTTTGGAACTGTCAGGCTGGAGTGCAGGAGGGGCAGGCGGAATTCCTGGTGTAGCGGTGAAATGCGTAGATATCAGGAGGAACACCGGTGGCGAAGGCGGCCTGCTGGACTGTAACTGACACTGAGGCTCGAAAGCGTGGGGAGCAAACAGG 11 0 9 5 0 +TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGCACGGCAAGCCTGATGTGAAAGCCCGCGGCCCAACCGCGGGACTGCATTGGGAACTACCGGGCTGGAGTGCCGGAGAGGTAGGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCCTACTGGACGGTGACTGACGCTGAGGCTCGAAAGCGTGGGGAGCAAACAGG 9 7 9 0 0 +TACGTAGGGAGCAAGCGTTATCCGGAATTATTGGGTGTAAAGGGTGCGTAGACGGGATAACAAGTTGGTTGTGAAACCCCTCGGCTCAACTGAGGAACTGCAACCAAAACTATTATTCTTGAGTGCAGGAGAGGAAAGTGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAAGAACACCGGTGGCGAAGGCGACTTTCTGGACTGTAACTGACGTTGAGGCACGAAAGTGTGGGGAGCAAACAGG 0 16 9 0 0 +TACGTAGGGGGCAAGCGTTATCCGGATTCACTGGGCGTAAAGGGAGCGCAGGCGGCATGGCAAGTCTGATGTGAAAACCCGGGGCCCAACCCCGGGAGTGCATTGGAAACTGCCAGGCTGGAGTGTCGGAGGGGTAAGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACGACTACTGACGCTGAGGCTCGAAAGCGTGGGGAGCAAACAGG 0 5 4 15 0 +TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTTATGTAAGTCTGAAGTGAAAGCCCGGGGCCCAACCCCGGGACTGCTTTGGAAACTGTGTAACTAGAGTACAGGAGGGGCAGGCGGAATTCCTGGTGTAGCGGTGAAATGCGTAGATATCAGGAGGAACACCGGCGGCGAAGGCGGCCTGCTGGACTGAAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGG 0 0 23 0 0 +TACGTAGGGGGCAAGCGTTATCCGGATTCACTGGGTGTAAAGGGAGCGTAGACGGCGACGCAAGTCTGGAGTGAAAGCCCGGGGCCCAACCCCGGGACTGCTCTGGAAACTGCGGCGCTGGAGTACGGGAGGGGCAGGCGGAATTCCTGGTGTAGCGGTGAAATGCGTAGATATCAGGAGGAACACCGGCGGCGAAGGCGGCCTGCTGGACCGTGACTGACGTTGAGGCTCGAGAGCGTGGGGAGCAAACAGG 0 0 23 0 0 +TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGGACTGCATTGGAAACTACCGAACTAGAGTGTCGGAGAGGTAAGTGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACGATAACTGACGCTGAGGCTCGAAAGCGTGGGGAGCAAACAGG 0 0 23 0 0 +TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGGCGGCCTGGCAAGTCTGGTGTGAAATACCGGGGCCCAACCCCGGGACTGCATTGGAAACTGCCAGGCTGGAGTGTCGGAGGGGCAGGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCGGTGGCGAAGGCGGCCTGCTGGACGACAACTGACGCTGAGGCTCGAAAGCGTGGGGAGCAAACAGG 22 0 0 0 0 +TACGTAGGTGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGTGTAGGCGGGAGAGCAAGTCAGACGTGAAATTCCAGGGCTCAACCCTGGAACTGCGTTTGAAACTGTTCTTCTTGAGTGATGGAGAGGCAGGCGGAATTCCGTGTGTAGCGGTGAAATGCGTAGATATACGGAGGAACACCAGTGGCGAAGGCGGCCTGCTGGACATTAACTGACGCTGAGGCGCGAAAGCGTGGGGAGCAAACAGG 13 9 0 0 0 +TACGTAGGGAGCAAGCGTTGTCCGGAATTACTGGGCGTAAAGGGCGCGTAGGTGGGCGCTTAAGTCAGGTGTGAAAACTCCGGGCTCAACCTGGAGACTGCACTTGAAACTGGGTGTCTTGAGGGCAGGAGAGGAAAGTGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGACTTTCTGGACTGTACCTGACGCTGAGGCGCGAAAGCGTGGGTAGCGAACGGG 0 5 11 6 0 +TACGTAGGTGGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGTAGGCGGGAAGCCAAGTCAGCTGTGAAAACTACGGGCTTAACCTGTAGACTGCAGTTGAAACTGGCTTTCTTGAGTGAAGTAGAGGTTGGCGGAATTCCGAGTGTAGCGGTGAAATGCGTAGATATTCGGAGGAACACCGGTGGCGAAGGCGGCCAACTGGGCTTTAACTGACGCTGAGGCTCGAAAGTGTGGGGAGCAAACAGG 21 0 0 0 0 +TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGAGTAGGTGGCAGAGCAAGTCCGAAGTGAAAACCCAAAGCTCAACTATGGGAATGCTTTAGAAACTGCTCAGCTAGAGTGCAGGAGAGGCAAGTGGAATTCTTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTGCTGGACTGTAACTGACACTGAGGCTCGAAAGCGTGGGGAGCAAACAGG 10 6 5 0 0 +TACGTAGGGGGCAAGCGTTATCCGGATTCACTGGGTGTAAAGGGAGCGTAGACGGCGACGCAAGTCTGGAGTGAAAGCCCGGGGCCCAACCCCGGGACTGCTCTGGAAACTGCGTCGCTGGAGTACGGGAGGGGCAGGCGGAATTCCTGGTGTAGCGGTGAAATGCGTAGATATCAGGAGGAACACCGGCGGCGAAGGCGGCCTGCTGGACCGTGACTGACGTTGAGGCTCGAGAGCGTGGGGAGCAAACAGG 9 0 0 12 0 +TACGTAGGATCCGAGCATTATCCGGAGTGACTGGGTGTAAAGAGTTGCGTAGGTGGCATAGTAAGTAGCTAGTGAAATCTGGTGGCTCAACCATTCAGACTATTAGCTAAACTGCTAAGCTCGAGACCGTTAGGGGTAACTGGAATTTCTAGTGTAGGAGTGAAATCCGTAGATATTAGAAGGAACACCGATAGCGTAGGCAGGTTACTGGGACGGTTCTGACACTAAGGCACGAAAGCGTAGGGAGCAAACGGG 0 3 0 18 0 +TACGTAGGAGGCAAGCGTTATCCGGAATGACTGGGCGTAAAGGGTGCGTAGGTGGTTTGACAAGTTGGTAGCGTAACTCCGAGGCTTAACCTCGGCACTACTACCAAAACTGTTGGACTCGAGTGCAGGAGGGGCAAATGGAATTCCTAGTGTAGCGGTGGAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGATTTGCTGGACTGTAACTGACACTGAGGCACGAAAGCGTGGGGAGCAAACAGG 19 0 0 0 0 +TACGTAGGTGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGTGTAGGCGGGACTGCAAGTCAGATGTGAAAACCACGGGCTCAACCTGTGGCCTGCATTTGAAACTGTAGTTCTTGAGTACTGGAGAGGCAGACGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGTCTGCTGGACAGCAACTGACGCTGAGGCGCGAAAGCGTGGGGAGCAAACAGG 19 0 0 0 0 +TACGTAGGGGGCGAGCGTTATCCGGATTCACTGGGCGTAAAGGGAGCGCAGGCGGCACGGCAAGTCCGGTGTGAAAGCCCGGGGCCCAACCCCGGGACTGCACTGGAAACTGCCGGGCTGGAGTGTCGGAGGGGCAGGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCGGTGGCGAAGGCGGCCTGCTGGACGACGACTGACGCTGAGGCTCGAAAGCGTGGGGAGCAAACAGG 19 0 0 0 0 +TACGTAGGGGGCAAGCGTTGTCCGGAATGACTGGGCGTAAAGGGAGTGTAGGCGGCTCATCAAGTTAGATGTGAAATCCCGCGGCTTAACCGCGGAACTGCATCTAAAACTGGTAAGCTGGAGTGCAGGAGAGGTGAGTGGAATTCCTAGTGTAGCGGTGGAATGCGTAGATATTAGGAGGAACACCAGAGGCGAAGGCGACTCACTGGACTGTAACTGACGCTGAGGCTCGAAAGCGTGGGGAGCAAACAGG 19 0 0 0 0 +TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTGATGCAAGTCAGAAGTGAAAGCCCGGGGCTCAACTCCGGGACTGCTTTTGAAACTGTGTGACTGGAGTGCAGGAGAGGTAAGCGGAATTCCTGGTGTAGCGGTGAAATGCGTAGATATCAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACTGTAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGG 10 0 0 9 0 +TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTATGGCAAGTCTGATGTGAAAACCCGGGGCTCAACCCCGGGACTGCATTGGAAACTGTCGGACTAGAGTGTCGGAGAGGCAAGTGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTGCTGGACGATGACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGG 0 14 0 5 0 +TACGTAGGGGGCAAGCGTTATCCGGATTCACTGGGCGTAAAGGGAGCGCAGGCGGCACGGCAAGTCTGGTGTGAAAGCCCGGGGCCCAACCCCGGGACTGCATTGGAAACTGCCGGGCTGGAGTGCCGGAGGGGTAAGCGGAATTCCTGGTGTAGCGGTGAAATGCGCAGATATCAGGAAGAACACCGGTGGCGGAGGCGGCTTACTGGACGGCAACTGACGCTGAGGCTCGAAAGCGTGGGGAGCAAACAGG 0 0 19 0 0 +TACGTAGGGAGCAAGCGTTGTCCGGAATTACTGGGCGTAAAGGGTGCGTAGGCGGCGCAGTTAGTCAGAAGTGAAATATCGGGGCTTAACCCCGGGGCTGCTTCTGATACTGCTGTGCTCGAGTGCAGGAGAGGTAAGTGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGACTTACTGGACTGTAACTGACGCTGAGGCACGAAAGCGTGGGTAGCAAACAGG 7 5 6 0 0 +TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCAATACAAGTCGGAAGTGAAATACCCGGGCTCAACCTGGGAACTGCTTTGGAAACTGTATGGCTGGAGTGCTGGAGAGGTAAGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACAGTAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGG 0 0 18 0 0 +TACGTAGGGGGCAAGCGTTATCCGGAATTACTGGGTGTAAAGGGAGAGTAGGCGGCAAGGTAAGCGATATGTGAAAGCCTTAGGCTTAACCAAAGGATTGCATAACGAACTATCTAGCTAGAGTACAGGAGAGGAAAGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAAGAACACCAGTGGCGAAGGCGGCTTTCTGGACTGAAACTGACGCTGAGGCTCGAAAGCGTGGGGAGCGAACAGG 17 0 0 0 0 +TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTTAATCAAGTCAGAAGTGAAAACCCAGGGCTCAACCTTGGGACTGCTTTTGAAACTGAGTAGCTAGAGTGCAGGAGAGGTAAGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACTGTAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGG 16 0 0 0 0 +TACGTAGGGGGCGAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGCGTGGCAAGTCAGATGTGAAAACCCGGGGCCCAACCCCGGGACTGCATTTGAAACTGCCATGCTGGAGTGCCGGAGAGGTAAGTGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACGGTAACTGACGCTGAGGCTCGAAAGCGTGGGGAGCAAACAGG 7 0 0 9 0 +TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCAATGCAAGCCAGATGTGAAAACCCGCAGCTCAACTGGGGGAGTGCATTTGGAACTGTGTAGCTAGAGTGCAGGAGAGGTAAGCGGAATTCCTGGTGTAGCGGTGAAATGCGTAGATATCAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACTGTAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGG 0 0 16 0 0 +TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGGTGGCAGGGCAAGTCAGATGTGAAAACCCGGGGCTCAACTCCGGGATTGCATTTGAAACTGTCCGGCTGGAGTGCAGGAGAGGTAAGTGGAATTCCTAGTGTAGCGGTGAAATGCATAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACTGTAACTGACACTGAGGCTCGAAAGCGTGGGGAGCAAACAGG 0 0 0 16 0 +TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCAGGCGGTTAGATAAGTCTGAAGTTAAAGGCTGTGGCTTAACCATAGTAGGCTTTGGAAACTGTTTAACTTGAGTGCAAGAGGGGAGAGTGGAATTCCATGTGTAGCGGTGAAATGCGTAGATATATGGAGGAACACCGGTGGCGAAAGCGGCTCTCTGGCTTGTAACTGACGCTGAGGCTCGAAAGCGTGGGGAGCAAACAGG 0 0 0 0 16 +TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGCGCGTTAAGCCTGGTGTGAAAGCCCGCGGCCCAACCGCGGGACTGCATTGGGAACTGGCGCGCTGGAGTGCCGGAGGGGTAGGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCCTACTGGACGGTGACTGACGCTGAGGCTCGAAAGCGTGGGGAGCAAACAGG 7 0 8 0 0 +TACGTAGGTGGCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGTGCGCAGGCGGTTTGTTAGGTCTAAAATTAAAGTCCGAAGCTTAACTTCGGTTCGTTTTAGAAACCGGCAGGCTTGAGTATGGTAGAGGCAAACGGAATTTCTAGTGTAGCGGTAAAATGCGTAGATATTAGAAGGAACACCAGTGGCGAAGGCGGTTTGCTGGGCCATTACTGACGCTCATGCACGAAAGCGTGGGGAGCAAATAGG 0 15 0 0 0 +TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCGAAGAAAGTCTGAAGTGAAAGCCCGCGGCTTAACCGCGGAACTGCTTTGGAAACTTTTTTGCTGGAGTACCGGAGAGGTAAGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACGGTAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGG 0 0 15 0 0 +TACGTAGGGAGCGAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGTAGGCGGGACTGCAAGTTGGGTGTCAAATCTACCGGCTCAACCGGTAGCCGCACTCAAAACTGCAGTTCTTGAGTGAAGTAGAGGCAGGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCCTGCTGGGCTTTTACTGACGCTGAGGCTCGAAAGTGTGGGGAGCAAACAGG 14 0 0 0 0 +TACGTAGGGGGCGAGCGTTGTCCGGAATGACTGGGCGTAAAGGGAGTGTAGGCGGCTCTTTAAGTTATATGTGAAATCCCGCGGCTTAACCGTGGAAGTGCATATAAAACTGGGGAGCTGGAGTGCAGGAGAGGTGAGTGGAATTCCTAGTGTAGCGGTGGAATGCGTAGATATTAGGAAGAACACCGGTGGCGAAGGCGGCTCACTGGACTGTAACTGACGCTGAGGCTCGAAAGCGTGGGGAGCAAACAGG 12 0 0 0 0 +TACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTAGGTGGTTTGTTAAGTTGGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATTCAAAACTGACTGACTAGAGTATGGTAGAGGGTGGTGGAATTTCCTGTGTAGCGGTGAAATGCGTAGATATAGGAAGGAACACCAGTGGCGAAGGCGACCACCTGGACTAATACTGACACTGAGGTGCGAAAGCGTGGGGAGCAAACAGG 8 2 0 2 0 +TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCAGTGCAAGTCTGGAGTGAAAGGCGGGGGCTCAACCCCCGGACTGCTCTGGAAACTGTACGGCTGGAGTGCAGGAGAGGTAAGTGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACTGTAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGG 0 0 12 0 0 +TACGTAAGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGTGAGTAGGCGGTTCGGCAAGTCATATGTGAAATACCTGGGCTCAACCCAGGAACCGCATAAGAAACTGTCGGACTTGAGTACAGGAGAGGTAAGCGGAATTCCCAGTGTAGCGGTGAAATGCGTAGATATTGGGAAGAACACCGGTGGCGAAGGCGGCTTACTGGACTGAAACTGACGCTGAGTCACGAAAGCGTGGGGAGCGAACAGG 11 0 0 0 0 +TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCAGGGCAAGTCTGGAGTGAAAGCCCGGGGCCCAACCCCGGGACTGCTTTGGAAACTGCCATGCTGGAGTGCTGGAGAGGTAAGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACAGTAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGG 0 0 11 0 0 +TACGTAGGGGGCAAGCGTTATCCGGAATTACTGGGTGTAAAGGGAGCGTAGACGGCAATGCAAGTCCGGAGTGGAATGCGGCAGCTCAACTGCCGAACTGCTCTGGAAACTGTATGGCTTGAGTGCAGGAGGGGTAAGCGGAATTCCTGGTGTAGCGGTGAAATGCGTAGATATCAGGAGGAACACCGGTGGCGAAGGCGGCTTACTGGACTGTAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGG 0 0 0 11 0 +TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGATAAGCAAGTCTGATGTGAAAATCCGGGGCCCAACCCCGGAATTGCATTGGAAACTGCATATCTAGAGTGTCGGAGAGGCAAGTGGAATTCCTGGTGTAGCGGTGAAATGCGTAGATATCAGGAGGAACACCAGTGGCGAAGGCGGCTTGCTGGACGATGACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGG 10 0 0 0 0 +TACAGAGGTCTCAAGCGTTGTTCGGAATCACTGGGCGTAAAGCGTGCGTAGGCTGTTTCGTAAGTCGTGTGTGAAAGGCGCGGGCTCAACCCGCGGACGGCACATGATACTGCGAGACTAGAGTAATGGAGGGGGAACCGGAATTCTCGGTGTAGCAGTGAAATGCGTAGATATCGAGAGGAACACTCGTGGCGAAGGCGGGTTCCTGGACATTAACTGACGCTGAGGCACGAAGGCCAGGGGAGCGAAAGGG 10 0 0 0 0 +TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCTGTGCAAGCCAGATGTGAAAGCCCGGGGCTCAACCCCGGGACTGCATTTGGAACTGTGTGGCTGGAGTGTCGGAGAGGCAGGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCCTGCTGGACGATGACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGG 10 0 0 0 0 +TACGTAGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGTGCAGCCGGAGAGACAAGTCAGATGTGAAATCCGCAGGCTCAACCTGCGAACTGCATTTGAAACTGTTTCCCTTGAGTATCGGAGAGGTCATCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAAGAACACCAGTGGCGAAGGCGGATGACTGGACGACAACTGACGGTGAGGCGCGAAAGCGTGGGGAGCAAACAGG 10 0 0 0 0 +TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCAGCGCAAGTCCGAAGTGAAAGCCCGGGGCCCAACCCCGGGACTGCTTTGGAAACTGTGAAGCTGGAGTGCGGGAGGGGCAGGCGGAATTCCTGGTGTAGCGGTGAAATGCGTAGATATCAGGAGGAACACCGGCGGCGAAGGCGGCCTGCTGGACCGTAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGG 0 0 10 0 0 +TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGGCGGCCTGGCAAGTCTGGTGTGAAAACCCGGGGCCCAACCCCGGGAGTGCATTGGAAACTGCCAGGCTGGAGTGCCGGAGGGGCAGGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCGGTGGCGAAGGCGGCCTGCTGGACGGCAACTGACGCTGAGGCTCGAAAGCGTGGGGAGCAAACAGG 0 0 9 0 0 +TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCAGGGCAAGCCAGAAGTGAAAACGCAGAGCTTAACTTTGCGACTGCTTTTGGAACTGCCCAGCTAGAGTACAGGAGAGGTAAGTGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACTGAGACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGG 0 0 0 9 0 +TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGGCGGCAAGGTAAGCCAGAAGTGAAAGCCCGTGGCTCAACTGCGGGACTGCTTTTGGAACTATCTAGCTAGATTGCAGGAGAGGTAAGTGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACTGTAAATGACGCTGAGGCTCGAAAGCGTGGGGAGCAAACAGG 0 8 0 0 0 +TACGTATGGTGCAAGCGTTATCCGGAATGACTGGGTGTAAAGGGAGCGTAGACGGTTGTACAAGTCTGATGTGAAAGCCCACGGCTCAACTGTGGGAGTGCATTGGAAACTGTAGAACTAGAGTATCGGAGAGGCAAGTGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTGCTGGACGAAAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGG 0 0 0 8 0 +TACGTAGGGGGCGAGCGTTATCCGGATTCATTGGGCGTAAAGCGCGCGTAGGCGGCCGCCTAAGCGGGACCTCTAACCCCGGGGCTCAACCCCGGGCCGGGTCCCGGACTGGGCGGCTCGAGTGCGGTAGAGGAGAGCGGAATTCCCGGTGTAGCGGTGGAATGCGCAGATATCGGGAAGAACACCGATGGCGAAGGCAGCTCTCTGGGCCGTCACTGACGCTGAGGCGCGAAAGCTGGGGGAGCGAACAGG 3 4 0 0 0 +TACGTAGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGTGTAGGCGGAGCTGCAAGTCAGATGTGAAATCCCGGGGCTCAACCCCGGAACTGCATTTGAAACTGTAGCCCTTGAGTATCGGAGAGGCAGGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCCTGCTGGACGACAACTGACGCTGAGGCGCGAAAGCGTGGGGAGCAAACAGG 0 7 0 0 0 +TACGTAGGGGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGTACGTAGGCGGTCTGTTAAGCGCAAGGTGAAAGGCATAGGCTCAACCAATGTGAGCCTTGCGAACTGGCAGACTTGAGTGCAGGAGAGGAAAGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCGGTGGCGAAGGCGGCTTTCTGGACTGAAACTGACGCTGAGGTACGAAAGCGTGGGGAGCAAACAGG 0 3 0 4 0 +TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCTAATCAAGTCAGAAGTGAAAACCCAGGGCTCAACCTTGGGACTGCTTTTGAAACTGAGTAGCTAGAGTGCAGGAGAGGTAAGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACTGTAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGG 0 0 7 0 0 +TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCGATGCAAGCCAGATGTGAAAGCCCGCGGCTCAACTGCGGTAGTGCATTTGGAACTGTATGGCTGGAGTGCAGGAGAGGCAGGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCCTGCTGGACTGTAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGG 0 0 0 7 0 +TACGTAGGTGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGTGTAGGCGGGACTGCAAGTCAGATGTGAAACCCATGGGCTCAACCCATGGCCTGCATTTGAAACTGTAGTTCTTGAGTGATGGAGAGGCAGGCGGAATTCCGTGTGTAGCGGTGAAATGCGTAGATATACGGAGGAACACCAGTGGCGAAGGCGGCCTGCTGGACATTAACTGACGCTGAGGCGCGAAAGCGTGGGGAGCAAACAGG 6 0 0 0 0 +TACGTAGGGAGCGAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGTGTAGGCGGGATGGCAAGTCAGGCGTGAAAACTATGGGCTCAACCCATAGCCTGCGTTTGAAACTGTCGTTCTTGAGTGATGGAGAGGCAAGCGGAATTCCGTGTGTAGCGGTGAAATGCGTAGATATACGGAGGAACACCAGTGGCGAAGGCGGCTTGCTGGACATTAACTGACGCTGAGGCGCGAAAGCGTGGGGAGCAAACAGG 6 0 0 0 0 +TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTTCTGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCGCGGGACTGCATTGGAAACTGCGGGACTGGAGTGTCGGAGGGGTAAGCGGAATTCCTGGTGTAGCGGTGAAATGCGTAGATATCAGGAGGAACACCGGTGGCGAAGGCGGCTTACTGGACGATGACTGACGCTGAGGCTCGAAAGCGTGGGGAGCAAACAGG 0 0 6 0 0 +TACGTAGGGAGCGAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGTAGGCGGGATTGCAAGTTGGATGTGAAAACTGCGGGCTCAACCCGGAGAGTGCATTCAAAACTGCGATTCTTGAGTGAAGTAGAGGCAAGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTGCTGGGCTTTTACTGACGCTGAGGCTCGAAAGTGTGGGGAGCAAACAGG 5 0 0 0 0 +TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGGCGGCAGTGCAAGTCAGAAGTGAAAGCCCGGGGCTCAACTCCGGGACTGCTTTTGAAACTGTACAGCTTGATTGCAGGAGAGGCAAGTGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTGCTGGACTGTAAATGACGCTGAGGCTCGAAAGCGTGGGGAGCAAACAGG 0 0 0 5 0 +TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCTGTGCAAGTCTGAAGTGAAAGGCCGGGGCCCAACCCCGGGAGTGCTTTGGAAACTGTGCGGCTAGAGTGTCGGAGAGGCAAGTGGAATTCCCAGTGTAGCGGTGAAATGCGTAGATATTGGGAGGAACACCAGTGGCGAAGGCGTCTTGCTGGACGATGACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGG 4 0 0 0 0 +TACGTAGGGGGCGAGCGTTGTCCGGAATGACTGGGCGTAAAGGGAGTGTAGGCGGCCTTTTAAGTTATGTGTGAAAGCCCACGGCTCAACTGTGGAATTGCACATAAAACTGGGAGGCTGGAGTGCAGGAGAGGTAAGCGGAATTTCCAGTGTAGCGGTGGAATGCGTAGATATTGGGAAGAACACCGGAGGCGAAGGCGGCTTACTGGACTGTAACTGACGCTGAGGCTCGAAAGCGTGGGGAGCAAACAGG 4 0 0 0 0 +GACGGGGGGGGCAAGTGTTCTTCGGAATGACTGGGCGTAAAGGGCACGTAGGCGGTGAATCGGGTTGAAAGTGAAAGTCGCCAAAAAGTGGCGGAATGCTCTCGAAACCAATTCACTTGAGTGAGACAGAGGAGAGTGGAATTTCGTGTGTAGGGGTGAAATCCGTAGATCTACGAAGGAACGCCAAAAGCGAAGGCAGCTCTCTGGGTCCCTACCGACGCTGGGGTGCGAAAGCATGGGGAGCGAACAGG 4 0 0 0 0 +TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCAGCGCAAGCCTGGAGTGAAAGGATGGGGCCCAACCCCATGACTGCTCTGGGAACTGTGCGGCTAGAGTGCCGGAGGGGTAAGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACGGTAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGG 0 4 0 0 0 +TACGTAGGTGGCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGTGCGCAGGCGGTTTGTTAAGTCTAAAATCAAAGCCCGAAGCTTAACTTCGGTTCGTTTTAGAAACTGGCAGGCTTGAGTATGGTAGAGGCAAACGGAATTTCTAGTGTAGCGGTAAAATGCGTAGATATTAGAAGGAACACCAGTGGCGAAGGCGGTTTGCTGGGCCATTACTGACGCTCATGCACGAAAGCGTGGGGAGCAAATAGG 0 0 4 0 0 +TACGTAGGGGGCGAGCGTTATCCGGATTCATTGGGCGTAAAGCGCGCGTAGGCGGCCGCCTAAGCGGAACCTCTAACCCCGGGGCTCAACCTCGGGCCGGGTTCCGGACTGGGCGGCTCGAGTGCGGTAGAGGAGAGCGGAATTCCCGGTGTAGCGGTGGAATGCGCAGATATCGGGAAGAACACCGATGGCGAAGGCAGCTCTCTGGGCCGTCACTGACGCTGAGGCGCGAAAGCTGGGGGAGCGAACAGG 0 0 0 4 0 +TACGTAGGTGGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGTAGGCGGGAAAGCAAGTTGAATGTTTAAGGTACGGGCTCAACCTGTACAAGCGTTCAAAACTGTTTTTCTTGAGTGGAGTAGAGGTAAGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAAATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGGCTTTAACTGACGCTGAGGCTCGAAAGCGTGGGTAGCAAACAGG 3 0 0 0 0 +TACGTAGGGAGCGAGCGTTATCCGGAATTATTGGGTGTAAAGGGTGCGTAGACGGGAAAACAAGTTAGTTGTGAAATCCCTCGGCTTAACTGAGGAACTGCAACTAAAACTATTTTTCTTGAGTGCAGGAGAGGTAAGTGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGACTTACTGGACTGTAACTGACGTTGAGGCACGAAAGTGTGGGGAGCAAACAGG 0 0 0 3 0 diff --git a/workflows/computational-chemistry/fragment-based-docking-scoring/.dockstore.yml b/workflows/computational-chemistry/fragment-based-docking-scoring/.dockstore.yml index e89851830..5438650c8 100644 --- a/workflows/computational-chemistry/fragment-based-docking-scoring/.dockstore.yml +++ b/workflows/computational-chemistry/fragment-based-docking-scoring/.dockstore.yml @@ -1,7 +1,13 @@ version: 1.2 workflows: - name: main - primaryDescriptorPath: /fragment-based-docking-scoring.ga subclass: Galaxy + publish: true + primaryDescriptorPath: /fragment-based-docking-scoring.ga testParameterFiles: - /fragment-based-docking-scoring-tests.yml + authors: + - name: Simon Bray + orcid: 0000-0002-0621-6705 + - name: Tim Dudgeon + orcid: 0000-0001-6879-5194 diff --git a/workflows/computational-chemistry/fragment-based-docking-scoring/CHANGELOG.md b/workflows/computational-chemistry/fragment-based-docking-scoring/CHANGELOG.md index 63f0303ce..11c5f4801 100644 --- a/workflows/computational-chemistry/fragment-based-docking-scoring/CHANGELOG.md +++ b/workflows/computational-chemistry/fragment-based-docking-scoring/CHANGELOG.md @@ -1,5 +1,15 @@ # Changelog +## [0.1.5] 2023-11-20 + +- Fix author in dockstore + +## [0.1.4] 2023-02-14 + +### Fixed +- Fix the changeset revisions for 2 tool shed repositories +- Sync version of ``openbabel_compund_convert`` step to newest version + ## [0.1.3] 2022-05-25 ### Changed diff --git a/workflows/computational-chemistry/fragment-based-docking-scoring/fragment-based-docking-scoring.ga b/workflows/computational-chemistry/fragment-based-docking-scoring/fragment-based-docking-scoring.ga index 57b2d147e..e80c448f5 100644 --- a/workflows/computational-chemistry/fragment-based-docking-scoring/fragment-based-docking-scoring.ga +++ b/workflows/computational-chemistry/fragment-based-docking-scoring/fragment-based-docking-scoring.ga @@ -16,7 +16,7 @@ "format-version": "0.1", "license": "MIT", "name": "Fragment-based virtual screening using rDock for docking and SuCOS for pose scoring", - "release": "0.1.3", + "release": "0.1.5", "steps": { "0": { "annotation": "Number of docking poses to generate per input compound", @@ -491,7 +491,7 @@ }, "tool_id": "toolshed.g2.bx.psu.edu/repos/bgruening/rxdock_rbcavity/rxdock_rbcavity/2013.1.1_148c5bd1+galaxy0", "tool_shed_repository": { - "changeset_revision": "7e5c2e4bc227", + "changeset_revision": "0848d3b1a46f", "name": "rxdock_rbcavity", "owner": "bgruening", "tool_shed": "toolshed.g2.bx.psu.edu" @@ -504,7 +504,7 @@ }, "12": { "annotation": "", - "content_id": "toolshed.g2.bx.psu.edu/repos/bgruening/openbabel_compound_convert/openbabel_compound_convert/2.4.2.2.0", + "content_id": "toolshed.g2.bx.psu.edu/repos/bgruening/openbabel_compound_convert/openbabel_compound_convert/3.1.1+galaxy0", "errors": null, "id": 12, "input_connections": { @@ -539,7 +539,7 @@ "output_name": "outfile" } }, - "tool_id": "toolshed.g2.bx.psu.edu/repos/bgruening/openbabel_compound_convert/openbabel_compound_convert/2.4.2.2.0", + "tool_id": "toolshed.g2.bx.psu.edu/repos/bgruening/openbabel_compound_convert/openbabel_compound_convert/3.1.1+galaxy0", "tool_shed_repository": { "changeset_revision": "e2c36f62e22f", "name": "openbabel_compound_convert", @@ -547,7 +547,7 @@ "tool_shed": "toolshed.g2.bx.psu.edu" }, "tool_state": "{\"appendtotitle\": \"\", \"dative_bonds\": \"false\", \"infile\": {\"__class__\": \"ConnectedValue\"}, \"oformat\": {\"oformat_opts_selector\": \"sdf\", \"__current_case__\": 58, \"sdf_exp_h\": \"false\", \"sdf_no_prop\": \"false\", \"sdf_wedge_bonds\": \"false\", \"sdf_alias_out\": \"false\", \"gen2d\": \"false\", \"gen3d\": \"true\"}, \"ph\": \"7.4\", \"remove_h\": \"false\", \"split\": \"false\", \"unique\": {\"unique_opts_selector\": \"\", \"__current_case__\": 0}, \"__page__\": null, \"__rerun_remap_job_id__\": null}", - "tool_version": "2.4.2.2.0", + "tool_version": "3.1.1+galaxy0", "type": "tool", "uuid": "7f069b7b-a0c0-474a-9e33-052ab9318658", "workflow_outputs": [] @@ -651,7 +651,7 @@ "post_job_actions": {}, "tool_id": "toolshed.g2.bx.psu.edu/repos/bgruening/rxdock_rbdock/rxdock_rbdock/2013.1.1_148c5bd1+galaxy0", "tool_shed_repository": { - "changeset_revision": "c362398df83b", + "changeset_revision": "b34d068c2782", "name": "rxdock_rbdock", "owner": "bgruening", "tool_shed": "toolshed.g2.bx.psu.edu" diff --git a/workflows/computational-chemistry/gromacs-dctmd/.dockstore.yml b/workflows/computational-chemistry/gromacs-dctmd/.dockstore.yml index ef8832d84..35f44991f 100644 --- a/workflows/computational-chemistry/gromacs-dctmd/.dockstore.yml +++ b/workflows/computational-chemistry/gromacs-dctmd/.dockstore.yml @@ -1,7 +1,11 @@ version: 1.2 workflows: - name: main - primaryDescriptorPath: /gromacs-dctmd.ga subclass: Galaxy + publish: true + primaryDescriptorPath: /gromacs-dctmd.ga testParameterFiles: - /gromacs-dctmd-tests.yml + authors: + - name: Simon Bray + orcid: 0000-0002-0621-6705 diff --git a/workflows/computational-chemistry/gromacs-dctmd/CHANGELOG.md b/workflows/computational-chemistry/gromacs-dctmd/CHANGELOG.md index d609c3858..321fb05c1 100644 --- a/workflows/computational-chemistry/gromacs-dctmd/CHANGELOG.md +++ b/workflows/computational-chemistry/gromacs-dctmd/CHANGELOG.md @@ -1,5 +1,18 @@ # Changelog +## [0.1.5] 2023-11-20 + +- Fix author in dockstore + +## [0.1.4] 2023-11-10 + +### Automatic update +- `toolshed.g2.bx.psu.edu/repos/chemteam/gmx_setup/gmx_setup/2021.3+galaxy0` was updated to `toolshed.g2.bx.psu.edu/repos/chemteam/gmx_setup/gmx_setup/2022+galaxy0` +- `toolshed.g2.bx.psu.edu/repos/chemteam/gmx_solvate/gmx_solvate/2021.3+galaxy0` was updated to `toolshed.g2.bx.psu.edu/repos/chemteam/gmx_solvate/gmx_solvate/2022+galaxy0` +- `toolshed.g2.bx.psu.edu/repos/chemteam/gmx_em/gmx_em/2021.3+galaxy1` was updated to `toolshed.g2.bx.psu.edu/repos/chemteam/gmx_em/gmx_em/2022+galaxy0` +- `toolshed.g2.bx.psu.edu/repos/chemteam/gmx_makendx/gmx_makendx/2021.3+galaxy0` was updated to `toolshed.g2.bx.psu.edu/repos/chemteam/gmx_makendx/gmx_makendx/2022+galaxy0` +- `toolshed.g2.bx.psu.edu/repos/chemteam/gmx_sim/gmx_sim/2021.3+galaxy2` was updated to `toolshed.g2.bx.psu.edu/repos/chemteam/gmx_sim/gmx_sim/2022+galaxy0` + ## [0.1.3] 2022-05-25 ### Changed diff --git a/workflows/computational-chemistry/gromacs-dctmd/gromacs-dctmd.ga 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"uuid": "506adfe5-8c0c-41d1-8a13-42f8821677de", + "uuid": "612d572b-541f-4e76-9a9a-d477abd10a07", + "when": null, "workflow_outputs": [] }, - "31": { + "30": { "annotation": "", "content_id": "toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_sed_tool/1.1.1", "errors": null, - "id": 31, + "id": 30, "input_connections": { "infile": { - "id": 28, + "id": 27, "output_name": "ndx" } }, @@ -2150,14 +1862,8 @@ } ], "position": { - "bottom": 329.1000061035156, - "height": 113.60000610351562, - "left": -263.5, - "right": -63.5, - "top": 215.5, - "width": 200, - "x": -263.5, - "y": 215.5 + "left": 1440.0, + "top": 670.0 }, "post_job_actions": { "HideDatasetActionoutput": { @@ -2168,7 +1874,7 @@ }, "tool_id": "toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_sed_tool/1.1.1", "tool_shed_repository": { - "changeset_revision": "ddf54b12c295", + "changeset_revision": "d698c222f354", "name": "text_processing", "owner": "bgruening", "tool_shed": "toolshed.g2.bx.psu.edu" @@ -2177,6 +1883,63 @@ "tool_version": "1.1.1", "type": "tool", "uuid": "95cac327-99f3-455e-8765-a7db33343894", + "when": null, + "workflow_outputs": [] + }, + "31": { + "annotation": "", + "content_id": "toolshed.g2.bx.psu.edu/repos/bgruening/add_line_to_file/add_line_to_file/0.1.0", + "errors": null, + "id": 31, + "input_connections": { + "infile": { + "id": 29, + "output_name": "outfile" + }, + "text_input": { + "id": 20, + "output_name": "out1" + } + }, + "inputs": [], + "label": null, + "name": "Add line to file", + "outputs": [ + { + "name": "outfile", + "type": "input" + } + ], + "position": { + "left": 1728.0, + "top": 863.0 + }, + "post_job_actions": { + "ChangeDatatypeActionoutfile": { + "action_arguments": { + "newtype": "mdp" + }, + "action_type": "ChangeDatatypeAction", + "output_name": "outfile" + }, + "HideDatasetActionoutfile": { + "action_arguments": {}, + "action_type": "HideDatasetAction", + "output_name": "outfile" + } + }, + "tool_id": "toolshed.g2.bx.psu.edu/repos/bgruening/add_line_to_file/add_line_to_file/0.1.0", + "tool_shed_repository": { + "changeset_revision": "8e251055b1a9", + "name": "add_line_to_file", + "owner": "bgruening", + "tool_shed": "toolshed.g2.bx.psu.edu" + }, + "tool_state": "{\"infile\": {\"__class__\": \"ConnectedValue\"}, \"options\": \"footer\", \"text_input\": {\"__class__\": \"ConnectedValue\"}, \"__page__\": null, \"__rerun_remap_job_id__\": null}", + "tool_version": "0.1.0", + "type": "tool", + "uuid": "506adfe5-8c0c-41d1-8a13-42f8821677de", + "when": null, "workflow_outputs": [] }, "32": { @@ -2186,11 +1949,11 @@ "id": 32, "input_connections": { "infile": { - "id": 31, + "id": 30, "output_name": "output" }, "text_input": { - "id": 0, + "id": 12, "output_name": "output" } }, @@ -2204,14 +1967,8 @@ } ], "position": { - "bottom": 242.5, - "height": 144, - "left": 24.5, - "right": 224.5, - "top": 98.5, - "width": 200, - "x": 24.5, - "y": 98.5 + "left": 1728.0, + "top": 553.0 }, "post_job_actions": { "HideDatasetActionoutfile": { @@ -2231,6 +1988,7 @@ "tool_version": "0.1.0", "type": "tool", "uuid": "45466bad-4528-4558-89e2-c80cd3d73029", + "when": null, "workflow_outputs": [] }, "33": { @@ -2240,7 +1998,7 @@ "id": 33, "input_connections": { "inputs": { - "id": 28, + "id": 27, "output_name": "ndx" }, "queries_0|inputs2": { @@ -2258,14 +2016,8 @@ } ], "position": { - "bottom": 174.5, - "height": 144, - "left": 302.5, - "right": 502.5, - "top": 30.5, - "width": 200, - "x": 302.5, - "y": 30.5 + "left": 2006.0, + "top": 485.0 }, "post_job_actions": { "ChangeDatatypeActionout_file1": { @@ -2283,7 +2035,7 @@ }, "tool_id": "toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_cat/0.1.1", "tool_shed_repository": { - "changeset_revision": "f46f0e4f75c4", + "changeset_revision": "d698c222f354", "name": "text_processing", "owner": "bgruening", "tool_shed": "toolshed.g2.bx.psu.edu" @@ -2292,20 +2044,21 @@ "tool_version": "0.1.1", "type": "tool", "uuid": "1a933020-6bde-4378-bae2-a401389e2d63", + "when": null, "workflow_outputs": [] }, "34": { "annotation": "", - "content_id": "toolshed.g2.bx.psu.edu/repos/chemteam/gmx_sim/gmx_sim/2021.3+galaxy2", + "content_id": "toolshed.g2.bx.psu.edu/repos/chemteam/gmx_sim/gmx_sim/2022+galaxy0", "errors": null, "id": 34, "input_connections": { "gro_input": { - "id": 29, + "id": 28, "output_name": "output1" }, "inps|cpt_in": { - "id": 29, + "id": 28, "output_name": "output5" }, "inps|ndx_in": { @@ -2313,11 +2066,11 @@ "output_name": "out_file1" }, "sets|mdp|mdp_input": { - "id": 30, + "id": 31, "output_name": "outfile" }, "top_input": { - "id": 22, + "id": 21, "output_name": "output2" } }, @@ -2356,14 +2109,8 @@ } ], "position": { - "bottom": 693.8999938964844, - "height": 458.3999938964844, - "left": 590.5, - "right": 790.5, - "top": 235.5, - "width": 200, - "x": 590.5, - "y": 235.5 + "left": 2294.0, + "top": 690.0 }, "post_job_actions": { "ChangeDatatypeActionoutput8": { @@ -2379,17 +2126,18 @@ "output_name": "output7_1" } }, - "tool_id": "toolshed.g2.bx.psu.edu/repos/chemteam/gmx_sim/gmx_sim/2021.3+galaxy2", + "tool_id": "toolshed.g2.bx.psu.edu/repos/chemteam/gmx_sim/gmx_sim/2022+galaxy0", "tool_shed_repository": { - "changeset_revision": "525ca7c8065f", + "changeset_revision": "c0c9a5024177", "name": "gmx_sim", "owner": "chemteam", "tool_shed": "toolshed.g2.bx.psu.edu" }, - "tool_state": "{\"__job_resource\": {\"__job_resource__select\": \"yes\", \"__current_case__\": 1, \"gpu\": \"1\"}, \"capture_log\": \"true\", \"gro_input\": {\"__class__\": \"ConnectedValue\"}, \"inps\": {\"cpt_in\": {\"__class__\": \"ConnectedValue\"}, \"itp_in\": {\"__class__\": \"RuntimeValue\"}, \"ndx_in\": {\"__class__\": \"ConnectedValue\"}}, \"mxw\": \"0\", \"outps\": {\"traj\": \"xtc\", \"str\": \"gro\", \"cpt_out\": \"false\", \"edr_out\": \"false\", \"xvg_out\": \"true\", \"tpr_out\": \"true\"}, \"sets\": {\"ensemble\": \"nvt\", \"mdp\": {\"mdpfile\": \"custom\", \"__current_case__\": 0, \"mdp_input\": {\"__class__\": \"ConnectedValue\"}}}, \"top_input\": {\"__class__\": \"ConnectedValue\"}, \"__page__\": null, \"__rerun_remap_job_id__\": null}", - "tool_version": "2021.3+galaxy2", + "tool_state": "{\"__job_resource\": {\"__current_case__\": 1, \"__job_resource__select\": \"yes\", \"gpu\": \"1\"}, \"capture_log\": true, \"gro_input\": {\"__class__\": \"ConnectedValue\"}, \"inps\": {\"cpt_in\": {\"__class__\": \"ConnectedValue\"}, \"itp_in\": {\"__class__\": \"RuntimeValue\"}, \"ndx_in\": {\"__class__\": \"ConnectedValue\"}}, \"mxw\": \"0\", \"outps\": {\"traj\": \"xtc\", \"str\": \"gro\", \"cpt_out\": \"false\", \"edr_out\": \"false\", \"xvg_out\": \"true\", \"tpr_out\": \"true\"}, \"sets\": {\"ensembleselect\": {\"ensemble\": \"nvt\", \"__current_case__\": 0, \"startvel\": \"false\"}, \"mdp\": {\"mdpfile\": \"custom\", \"__current_case__\": 0, \"mdp_input\": {\"__class__\": \"ConnectedValue\"}}}, \"top_input\": {\"__class__\": \"ConnectedValue\"}, \"__page__\": null, \"__rerun_remap_job_id__\": null}", + "tool_version": "2022+galaxy0", "type": "tool", "uuid": "c724b44d-5a73-4e92-8406-bf45ebbdb716", + "when": null, "workflow_outputs": [ { "label": "XVG files", @@ -2448,14 +2196,8 @@ } ], "position": { - "bottom": 594.8999938964844, - "height": 174.39999389648438, - "left": 868.5, - "right": 1068.5, - "top": 420.5, - "width": 200, - "x": 868.5, - "y": 420.5 + "left": 2572.0, + "top": 875.0 }, "post_job_actions": {}, "tool_id": "toolshed.g2.bx.psu.edu/repos/chemteam/biomd_neqgamma/biomd_neqgamma/0.1.5.2+galaxy1", @@ -2469,6 +2211,7 @@ "tool_version": "0.1.5.2+galaxy1", "type": "tool", "uuid": "7446ff96-7c10-4363-9067-045c05dfddf6", + "when": null, "workflow_outputs": [ { "label": "Friction data", @@ -2486,4 +2229,4 @@ "tags": [], "uuid": "7950b716-2bc9-4cb6-8472-6506c702adea", "version": 1 -} +} \ No newline at end of file diff --git a/workflows/computational-chemistry/gromacs-mmgbsa/.dockstore.yml b/workflows/computational-chemistry/gromacs-mmgbsa/.dockstore.yml index 45dbc34b8..f05c0f5ac 100644 --- a/workflows/computational-chemistry/gromacs-mmgbsa/.dockstore.yml +++ b/workflows/computational-chemistry/gromacs-mmgbsa/.dockstore.yml @@ -1,7 +1,11 @@ version: 1.2 workflows: - name: main - primaryDescriptorPath: /gromacs-mmgbsa.ga subclass: Galaxy + publish: true + primaryDescriptorPath: /gromacs-mmgbsa.ga testParameterFiles: - /gromacs-mmgbsa-tests.yml + authors: + - name: Simon Bray + orcid: 0000-0002-0621-6705 diff --git a/workflows/computational-chemistry/gromacs-mmgbsa/CHANGELOG.md b/workflows/computational-chemistry/gromacs-mmgbsa/CHANGELOG.md index e749b2694..27519c696 100644 --- a/workflows/computational-chemistry/gromacs-mmgbsa/CHANGELOG.md +++ b/workflows/computational-chemistry/gromacs-mmgbsa/CHANGELOG.md @@ -1,5 +1,22 @@ # Changelog +## [0.1.5] 2023-11-27 + +### Automatic update +- `toolshed.g2.bx.psu.edu/repos/chemteam/gmx_setup/gmx_setup/2021.3+galaxy0` was updated to `toolshed.g2.bx.psu.edu/repos/chemteam/gmx_setup/gmx_setup/2022+galaxy0` +- `toolshed.g2.bx.psu.edu/repos/chemteam/gmx_editconf/gmx_editconf/2021.3+galaxy0` was updated to `toolshed.g2.bx.psu.edu/repos/chemteam/gmx_editconf/gmx_editconf/2022+galaxy0` +- `toolshed.g2.bx.psu.edu/repos/bgruening/split_file_to_collection/split_file_to_collection/0.5.0` was updated to `toolshed.g2.bx.psu.edu/repos/bgruening/split_file_to_collection/split_file_to_collection/0.5.1` +- `toolshed.g2.bx.psu.edu/repos/chemteam/gmx_solvate/gmx_solvate/2021.3+galaxy1` was updated to `toolshed.g2.bx.psu.edu/repos/chemteam/gmx_solvate/gmx_solvate/2022+galaxy0` +- `toolshed.g2.bx.psu.edu/repos/chemteam/gmx_em/gmx_em/2021.3+galaxy1` was updated to `toolshed.g2.bx.psu.edu/repos/chemteam/gmx_em/gmx_em/2022+galaxy0` +- `toolshed.g2.bx.psu.edu/repos/chemteam/gmx_sim/gmx_sim/2021.3+galaxy2` was updated to `toolshed.g2.bx.psu.edu/repos/chemteam/gmx_sim/gmx_sim/2022+galaxy0` +- `toolshed.g2.bx.psu.edu/repos/chemteam/md_converter/md_converter/1.9.6+galaxy0` was updated to `toolshed.g2.bx.psu.edu/repos/chemteam/md_converter/md_converter/1.9.7+galaxy0` +- `toolshed.g2.bx.psu.edu/repos/nml/collapse_collections/collapse_dataset/4.2` was updated to `toolshed.g2.bx.psu.edu/repos/nml/collapse_collections/collapse_dataset/5.1.0` + +## [0.1.4] 2023-11-20 + +- Fix author in dockstore +- Fix changeset_revision of gmx_solvate + ## [0.1.3] 2022-05-25 ### Changed diff --git a/workflows/computational-chemistry/gromacs-mmgbsa/gromacs-mmgbsa.ga b/workflows/computational-chemistry/gromacs-mmgbsa/gromacs-mmgbsa.ga index f2055ec37..3db8129e9 100644 --- a/workflows/computational-chemistry/gromacs-mmgbsa/gromacs-mmgbsa.ga +++ b/workflows/computational-chemistry/gromacs-mmgbsa/gromacs-mmgbsa.ga @@ -11,70 +11,60 @@ "format-version": "0.1", "license": "MIT", "name": "MMGBSA calculations with GROMACS", - "release": "0.1.3", + "release": "0.1.5", "steps": { "0": { - "annotation": "Size of the MMGBSA ensemble", + "annotation": "NaCl concentration", "content_id": null, "errors": null, "id": 0, "input_connections": {}, "inputs": [ { - "description": "Size of the MMGBSA ensemble", - "name": "Number of simulations" + "description": "NaCl concentration", + "name": "Salt concentration" } ], - "label": "Number of simulations", + "label": "Salt concentration", "name": "Input parameter", "outputs": [], "position": { - "bottom": 128.10000610351562, - "height": 102.60000610351562, - "left": 1, - "right": 201, - "top": 25.5, - "width": 200, - "x": 1, - "y": 25.5 + "left": 0, + "top": 184.0 }, "tool_id": null, - "tool_state": "{\"parameter_type\": \"integer\", \"optional\": false}", + "tool_state": "{\"default\": 0.0, \"parameter_type\": \"float\", \"optional\": true}", "tool_version": null, "type": "parameter_input", - "uuid": "0b26a4eb-6715-4e56-b8c4-1796486f91c5", + "uuid": "be1f6375-076e-4d61-a780-f867af394809", + "when": null, "workflow_outputs": [] }, "1": { - "annotation": "NaCl concentration", + "annotation": "Size of the MMGBSA ensemble", "content_id": null, "errors": null, "id": 1, "input_connections": {}, "inputs": [ { - "description": "NaCl concentration", - "name": "Salt concentration" + "description": "Size of the MMGBSA ensemble", + "name": "Number of simulations" } ], - "label": "Salt concentration", + "label": "Number of simulations", "name": "Input parameter", "outputs": [], "position": { - "bottom": 284.6999969482422, - "height": 82.19999694824219, - "left": -277, - "right": -77, - "top": 202.5, - "width": 200, - "x": -277, - "y": 202.5 + "left": 278, + "top": 7.0 }, "tool_id": null, - "tool_state": "{\"default\": 0.0, \"parameter_type\": \"float\", \"optional\": true}", + "tool_state": "{\"parameter_type\": \"integer\", \"optional\": false}", "tool_version": null, "type": "parameter_input", - "uuid": "be1f6375-076e-4d61-a780-f867af394809", + "uuid": "0b26a4eb-6715-4e56-b8c4-1796486f91c5", + "when": null, "workflow_outputs": [] }, "2": { @@ -93,20 +83,15 @@ "name": "Input dataset", "outputs": [], "position": { - "bottom": 402.6999969482422, - "height": 82.19999694824219, - "left": 279, - "right": 479, - "top": 320.5, - "width": 200, - "x": 279, - "y": 320.5 + "left": 556, + "top": 302.0 }, "tool_id": null, - "tool_state": "{\"optional\": false}", + "tool_state": "{\"optional\": false, \"tag\": null}", "tool_version": null, "type": "data_input", "uuid": "808dce0b-2da4-4cad-865e-5f264164b9a5", + "when": null, "workflow_outputs": [] }, "3": { @@ -125,20 +110,15 @@ "name": "Input parameter", "outputs": [], "position": { - "bottom": 502.3000030517578, - "height": 61.80000305175781, - "left": 279, - "right": 479, - "top": 440.5, - "width": 200, - "x": 279, - "y": 440.5 + "left": 556, + "top": 422.0 }, "tool_id": null, "tool_state": "{\"restrictions\": [\"tip3p\", \"tip4p\", \"tips3p\", \"tip5p\", \"spc\", \"spce\", \"none\"], \"parameter_type\": \"text\", \"optional\": false}", "tool_version": null, "type": "parameter_input", "uuid": "c556adbf-d074-4467-95dc-1c1966c5cff2", + "when": null, "workflow_outputs": [] }, "4": { @@ -157,20 +137,15 @@ "name": "Input parameter", "outputs": [], "position": { - "bottom": 602.3000030517578, - "height": 61.80000305175781, - "left": 279, - "right": 479, - "top": 540.5, - "width": 200, - "x": 279, - "y": 540.5 + "left": 556, + "top": 522.0 }, "tool_id": null, "tool_state": "{\"default\": -1.0, \"parameter_type\": \"float\", \"optional\": true}", "tool_version": null, "type": "parameter_input", "uuid": "a7af9e2f-f236-4ed4-b4e6-472d8542d8b5", + "when": null, "workflow_outputs": [] }, "5": { @@ -189,20 +164,15 @@ "name": "Input parameter", "outputs": [], "position": { - "bottom": 702.3000030517578, - "height": 61.80000305175781, - "left": 279, - "right": 479, - "top": 640.5, - "width": 200, - "x": 279, - "y": 640.5 + "left": 556, + "top": 622.0 }, "tool_id": null, "tool_state": "{\"restrictions\": [\"oplsaa\", \"gromos43a1\", \"amber96\", \"gromos53a6\", \"amber99sb\", \"gromos53a5\", \"gromos43a2\", \"amberGS\", \"charmm27\", \"amber03\", \"gromos54a7\", \"gromos45a3\", \"amber99\", \"amber94\"], \"parameter_type\": \"text\", \"optional\": false}", "tool_version": null, "type": "parameter_input", "uuid": "8c2f5860-bd30-4351-8bfb-1f9c42a92c4b", + "when": null, "workflow_outputs": [] }, "6": { @@ -221,20 +191,15 @@ "name": "Input dataset", "outputs": [], "position": { - 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name: main - primaryDescriptorPath: /protein-ligand-complex-parameterization.ga subclass: Galaxy + publish: true + primaryDescriptorPath: /protein-ligand-complex-parameterization.ga testParameterFiles: - /protein-ligand-complex-parameterization-tests.yml + authors: + - name: Simon Bray + orcid: 0000-0002-0621-6705 diff --git a/workflows/computational-chemistry/protein-ligand-complex-parameterization/CHANGELOG.md b/workflows/computational-chemistry/protein-ligand-complex-parameterization/CHANGELOG.md index 3be52e4a9..d7d08482f 100644 --- a/workflows/computational-chemistry/protein-ligand-complex-parameterization/CHANGELOG.md +++ b/workflows/computational-chemistry/protein-ligand-complex-parameterization/CHANGELOG.md @@ -1,5 +1,9 @@ # Changelog +## [0.1.4] 2023-11-20 + +- Fix author in dockstore + ## [0.1.3] 2022-05-25 ### Changed diff --git a/workflows/computational-chemistry/protein-ligand-complex-parameterization/protein-ligand-complex-parameterization.ga b/workflows/computational-chemistry/protein-ligand-complex-parameterization/protein-ligand-complex-parameterization.ga index 53ff9b1ed..090edbe2d 100644 --- a/workflows/computational-chemistry/protein-ligand-complex-parameterization/protein-ligand-complex-parameterization.ga +++ b/workflows/computational-chemistry/protein-ligand-complex-parameterization/protein-ligand-complex-parameterization.ga @@ -11,7 +11,7 @@ "format-version": "0.1", "license": "MIT", "name": "Create GRO and TOP complex files", - "release": "0.1.3", + "release": "0.1.4", "steps": { "0": { "annotation": "pH for protonating the ligand. Set to -1.0 to skip.", diff --git a/workflows/data-fetching/parallel-accession-download/.dockstore.yml b/workflows/data-fetching/parallel-accession-download/.dockstore.yml index 45cdce16d..8b6502719 100644 --- a/workflows/data-fetching/parallel-accession-download/.dockstore.yml +++ b/workflows/data-fetching/parallel-accession-download/.dockstore.yml @@ -1,7 +1,13 @@ version: 1.2 workflows: - name: main - primaryDescriptorPath: /parallel-accession-download.ga subclass: Galaxy + publish: true + primaryDescriptorPath: /parallel-accession-download.ga testParameterFiles: - /parallel-accession-download-tests.yml + authors: + - name: Marius van den Beek + orcid: 0000-0002-9676-7032 + - name: IWC + url: https://github.com/galaxyproject/iwc diff --git a/workflows/data-fetching/parallel-accession-download/CHANGELOG.md b/workflows/data-fetching/parallel-accession-download/CHANGELOG.md index cfbd7e6fc..5ebdf7b95 100644 --- a/workflows/data-fetching/parallel-accession-download/CHANGELOG.md +++ b/workflows/data-fetching/parallel-accession-download/CHANGELOG.md @@ -1,5 +1,45 @@ # Changelog +## [0.1.11] 2024-04-08 + +### Automatic update +- `toolshed.g2.bx.psu.edu/repos/iuc/sra_tools/fasterq_dump/3.0.10+galaxy0` was updated to `toolshed.g2.bx.psu.edu/repos/iuc/sra_tools/fasterq_dump/3.1.0+galaxy0` + +## [0.1.10] 2024-03-11 + +### Automatic update +- `toolshed.g2.bx.psu.edu/repos/iuc/sra_tools/fasterq_dump/3.0.8+galaxy1` was updated to `toolshed.g2.bx.psu.edu/repos/iuc/sra_tools/fasterq_dump/3.0.10+galaxy0` + +## [0.1.9] 2023-11-27 + +### Automatic update +- `toolshed.g2.bx.psu.edu/repos/bgruening/split_file_to_collection/split_file_to_collection/0.5.0` was updated to `toolshed.g2.bx.psu.edu/repos/bgruening/split_file_to_collection/split_file_to_collection/0.5.1` + +## [0.1.8] 2023-11-20 + +### Automatic update +- `toolshed.g2.bx.psu.edu/repos/iuc/sra_tools/fasterq_dump/3.0.8+galaxy0` was updated to `toolshed.g2.bx.psu.edu/repos/iuc/sra_tools/fasterq_dump/3.0.8+galaxy1` + +## [0.1.7] 2023-11-10 + +### Automatic update +- `toolshed.g2.bx.psu.edu/repos/iuc/sra_tools/fasterq_dump/3.0.5+galaxy3` was updated to `toolshed.g2.bx.psu.edu/repos/iuc/sra_tools/fasterq_dump/3.0.8+galaxy0` + +## [0.1.6] 2023-09-27 + +### Automatic update +- `toolshed.g2.bx.psu.edu/repos/iuc/sra_tools/fasterq_dump/3.0.5+galaxy1` was updated to `toolshed.g2.bx.psu.edu/repos/iuc/sra_tools/fasterq_dump/3.0.5+galaxy3` + +## [0.1.5] 2023-09-12 + +### Automatic update +- `toolshed.g2.bx.psu.edu/repos/iuc/sra_tools/fasterq_dump/3.0.5+galaxy0` was updated to `toolshed.g2.bx.psu.edu/repos/iuc/sra_tools/fasterq_dump/3.0.5+galaxy1` + +## [0.1.4] 2023-02-17 + +### Automatic update +- `toolshed.g2.bx.psu.edu/repos/iuc/sra_tools/fasterq_dump/2.11.0+galaxy1` was updated to `toolshed.g2.bx.psu.edu/repos/iuc/sra_tools/fasterq_dump/3.0.5+galaxy0` + ## [0.1.3] 2022-02-04 ### Automatic update diff --git a/workflows/data-fetching/parallel-accession-download/parallel-accession-download.ga b/workflows/data-fetching/parallel-accession-download/parallel-accession-download.ga index 0a064617e..a7caa9709 100644 --- a/workflows/data-fetching/parallel-accession-download/parallel-accession-download.ga +++ b/workflows/data-fetching/parallel-accession-download/parallel-accession-download.ga @@ -15,7 +15,7 @@ ], "format-version": "0.1", "license": "MIT", - "release": "0.1.3", + "release": "0.1.11", "name": "Parallel Accession Download", "steps": { "0": { @@ -34,25 +34,20 @@ "name": "Input dataset", "outputs": [], "position": { - "bottom": 634.3875122070312, - "height": 61, - "left": 387.4250183105469, - "right": 587.4250183105469, - "top": 573.3875122070312, - "width": 200, - "x": 387.4250183105469, - "y": 573.3875122070312 + "left": 0.0, + "top": 27.0 }, "tool_id": null, - "tool_state": "{\"optional\": false, \"format\": [\"txt\"]}", + "tool_state": "{\"optional\": false, \"format\": [\"txt\"], \"tag\": null}", "tool_version": null, "type": "data_input", "uuid": "e9e5605e-29e1-4f90-9693-0e40a2ddfd8f", + "when": null, "workflow_outputs": [] }, "1": { "annotation": "", - "content_id": "toolshed.g2.bx.psu.edu/repos/bgruening/split_file_to_collection/split_file_to_collection/0.5.0", + "content_id": "toolshed.g2.bx.psu.edu/repos/bgruening/split_file_to_collection/split_file_to_collection/0.5.1", "errors": null, "id": 1, "input_connections": { @@ -61,7 +56,12 @@ "output_name": "output" } }, - "inputs": [], + "inputs": [ + { + "description": "runtime parameter for tool Split file", + "name": "split_parms" + } + ], "label": "Split accessions to collection", "name": "Split file", "outputs": [ @@ -71,14 +71,8 @@ } ], "position": { - "bottom": 851.4000244140625, - "height": 132, - "left": 533.9375, - "right": 733.9375, - "top": 719.4000244140625, - "width": 200, - "x": 533.9375, - "y": 719.4000244140625 + "left": 146.51248168945312, + "top": 173.01251220703125 }, "post_job_actions": { "HideDatasetActionlist_output_txt": { @@ -87,17 +81,18 @@ "output_name": "list_output_txt" } }, - "tool_id": "toolshed.g2.bx.psu.edu/repos/bgruening/split_file_to_collection/split_file_to_collection/0.5.0", + "tool_id": "toolshed.g2.bx.psu.edu/repos/bgruening/split_file_to_collection/split_file_to_collection/0.5.1", "tool_shed_repository": { - "changeset_revision": "6cbe2f30c2d7", + "changeset_revision": "baabc30154cd", "name": "split_file_to_collection", "owner": "bgruening", "tool_shed": "toolshed.g2.bx.psu.edu" }, "tool_state": "{\"split_parms\": {\"select_ftype\": \"txt\", \"__current_case__\": 5, \"input\": {\"__class__\": \"ConnectedValue\"}, \"select_mode\": {\"mode\": \"chunk\", \"__current_case__\": 0, \"chunksize\": \"1\"}, \"newfilenames\": \"split_file\", \"select_allocate\": {\"allocate\": \"byrow\", \"__current_case__\": 2}}, \"__page__\": null, \"__rerun_remap_job_id__\": null}", - "tool_version": "0.5.0", + "tool_version": "0.5.1", "type": "tool", "uuid": "f8d776ff-e1ba-4bde-9b4b-392d825fd2b7", + "when": null, "workflow_outputs": [] }, "2": { @@ -121,14 +116,8 @@ } ], "position": { - "bottom": 678.3875122070312, - "height": 132, - "left": 725.4625244140625, - "right": 925.4625244140625, - "top": 546.3875122070312, - "width": 200, - "x": 725.4625244140625, - "y": 546.3875122070312 + "left": 338.0375061035156, + "top": 0.0 }, "post_job_actions": { "HideDatasetActiontext_param": { @@ -138,15 +127,16 @@ } }, "tool_id": "param_value_from_file", - "tool_state": "{\"input1\": {\"__class__\": \"ConnectedValue\"}, \"param_type\": \"text\", \"remove_newlines\": \"true\", \"__page__\": null, \"__rerun_remap_job_id__\": null}", + "tool_state": "{\"input1\": {\"__class__\": \"ConnectedValue\"}, \"param_type\": \"text\", \"remove_newlines\": true, \"__page__\": null, \"__rerun_remap_job_id__\": null}", "tool_version": "0.1.0", "type": "tool", "uuid": "570d0317-259f-42b1-91f4-74f050e754b5", + "when": null, "workflow_outputs": [] }, "3": { "annotation": "", - "content_id": "toolshed.g2.bx.psu.edu/repos/iuc/sra_tools/fasterq_dump/2.11.0+galaxy1", + "content_id": "toolshed.g2.bx.psu.edu/repos/iuc/sra_tools/fasterq_dump/3.1.0+galaxy0", "errors": null, "id": 3, "input_connections": { @@ -155,7 +145,12 @@ "output_name": "text_param" } }, - "inputs": [], + "inputs": [ + { + "description": "runtime parameter for tool Faster Download and Extract Reads in FASTQ", + "name": "input" + } + ], "label": "fasterq-dump", "name": "Faster Download and Extract Reads in FASTQ", "outputs": [ @@ -177,14 +172,8 @@ } ], "position": { - "bottom": 889.3624877929688, - "height": 242, - "left": 992.3500366210938, - "right": 1192.3500366210938, - "top": 647.3624877929688, - "width": 200, - "x": 992.3500366210938, - "y": 647.3624877929688 + "left": 604.9250183105469, + "top": 100.9749755859375 }, "post_job_actions": { "HideDatasetActionlist_paired": { @@ -208,17 +197,18 @@ "output_name": "output_collection_other" } }, - "tool_id": "toolshed.g2.bx.psu.edu/repos/iuc/sra_tools/fasterq_dump/2.11.0+galaxy1", + "tool_id": "toolshed.g2.bx.psu.edu/repos/iuc/sra_tools/fasterq_dump/3.1.0+galaxy0", "tool_shed_repository": { - "changeset_revision": "83c7d564b128", + "changeset_revision": "ba931a59dd59", "name": "sra_tools", "owner": "iuc", "tool_shed": "toolshed.g2.bx.psu.edu" }, - "tool_state": "{\"__job_resource\": {\"__current_case__\": 0, \"__job_resource__select\": \"no\"}, \"adv\": {\"minlen\": null, \"split\": \"--split-3\", \"skip_technical\": \"true\"}, \"input\": {\"input_select\": \"accession_number\", \"__current_case__\": 0, \"accession\": {\"__class__\": \"ConnectedValue\"}}, \"__page__\": null, \"__rerun_remap_job_id__\": null}", - "tool_version": "2.11.0+galaxy1", + "tool_state": "{\"__job_resource\": {\"__current_case__\": 0, \"__job_resource__select\": \"no\"}, \"adv\": {\"seq_defline\": \"@$sn/$ri\", \"minlen\": null, \"split\": \"--split-3\", \"skip_technical\": true}, \"input\": {\"input_select\": \"accession_number\", \"__current_case__\": 0, \"accession\": {\"__class__\": \"ConnectedValue\"}}, \"__page__\": null, \"__rerun_remap_job_id__\": null}", + "tool_version": "3.1.0+galaxy0", "type": "tool", "uuid": "4d328e7b-df7d-4d3e-a60f-1ea12925f317", + "when": null, "workflow_outputs": [] }, "4": { @@ -242,14 +232,8 @@ } ], "position": { - "bottom": 768.3624877929688, - "height": 112, - "left": 1319.425048828125, - "right": 1519.425048828125, - "top": 656.3624877929688, - "width": 200, - "x": 1319.425048828125, - "y": 656.3624877929688 + "left": 932.0000305175781, + "top": 109.9749755859375 }, "post_job_actions": { "TagDatasetActionoutput": { @@ -265,6 +249,7 @@ "tool_version": "1.1.0", "type": "tool", "uuid": "924d2152-5640-40cd-bec3-0ced45469d84", + "when": null, "workflow_outputs": [ { "label": "Paired End Reads", @@ -294,14 +279,8 @@ } ], "position": { - "bottom": 911.4125366210938, - "height": 112, - "left": 1326.4749755859375, - "right": 1526.4749755859375, - "top": 799.4125366210938, - "width": 200, - "x": 1326.4749755859375, - "y": 799.4125366210938 + "left": 939.0499572753906, + "top": 253.0250244140625 }, "post_job_actions": { "TagDatasetActionoutput": { @@ -317,6 +296,7 @@ "tool_version": "1.1.0", "type": "tool", "uuid": "946d9610-c363-4915-b93a-f074169ef812", + "when": null, "workflow_outputs": [ { "label": "Single End Reads", diff --git a/workflows/data-fetching/parallel-accession-download/test-data/SRR044777_head.fastq b/workflows/data-fetching/parallel-accession-download/test-data/SRR044777_head.fastq index 013984ddc..9c35cf9e4 100644 --- a/workflows/data-fetching/parallel-accession-download/test-data/SRR044777_head.fastq +++ b/workflows/data-fetching/parallel-accession-download/test-data/SRR044777_head.fastq @@ -1,10 +1,12 @@ -@SRR044777.1 F47USSH02H1LGA length=160 +@F47USSH02H1LGA/4 NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN -+SRR044777.1 F47USSH02H1LGA length=160 ++ !!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!! -@SRR044777.2 F47USSH02GNP1D length=320 +@F47USSH02GNP1D/4 CACGTAGTTAGCCGGTGCTTATTCTTCCGGTACCGTCAGCACGCAATGGTATTAACATCGCGCTTTTCTTCCCGGACAAAAGTCCTTTACAACCCGAAGGCCTTCTTCAGACACGCGGCATGGCTGGATCAGGGTTGCCCCCATTGTCCAAAATTCCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTGGCGGATCATCCTCTCAGACCCGCTACTGATCGTCGCCTTGGTAGGCCTTTACCCCACCAACCAGCTAATCAGATATCGGCCGCTCAAACAGCGCAAGGCACACAGGGGATAGGN -+SRR044777.2 F47USSH02GNP1D length=320 ++ IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIAAAAIIIHCCCCII@@@@IIIIHHHIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHIII?????IIIIIII@@@@HH6666IFIIIIIIIIHHHIIIIIIIIIIHHHIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIICCCIIIIIIIIIIIIIIIIIIIIIIII??===?@B>>IDCIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHIIIIIIHHHHIIIIII@@@@HIIII! -@SRR044777.3 F47USSH02JNWNH length=78 +@F47USSH02JNWNH/4 GGCCATGCCTGTACCTGGGGTCCCTACTGAGCCAGGATCAAACTCTCTGAGACTGCCAAGGCACACAGGGGGATAGGN ++ +AAIIIIIIIIIIIIIIACCCIHHHIIIIIIIIHHHHIIIHHHIIIIIIIIIIIIICCCCEAIIBB;4-----8<=<1 times" + text: "141221 (50.26%) aligned concordantly >1 times" MarkDuplicates metrics: element_tests: SRR891268_chr22_enriched: @@ -54,7 +55,7 @@ SRR891268_chr22_enriched: asserts: has_n_lines: - n: 255 + n: 223 MACS2 report: element_tests: SRR891268_chr22_enriched: @@ -62,7 +63,7 @@ - that: "has_text" text: "# tag size is determined as 47 bps" - that: "has_text" - text: "# total tags in treatment: 239626" + text: "# total tags in treatment: 232804" Coverage from MACS2 (bigwig): element_tests: SRR891268_chr22_enriched: @@ -75,35 +76,49 @@ SRR891268_chr22_enriched: asserts: has_n_lines: - n: 240 + n: 211 Nb of reads in summits +-500bp: element_tests: SRR891268_chr22_enriched: asserts: has_line: - line: "10107" + line: "8698" + bigwig_norm: + element_tests: + SRR891268_chr22_enriched: + asserts: + has_size: + value: 1130014 + delta: 100000 + bigwig_norm2: + element_tests: + SRR891268_chr22_enriched: + asserts: + has_size: + value: 1133795 + delta: 100000 'MultiQC on input dataset(s): Stats': element_tests: bowtie2: asserts: has_line: - line: "SRR891268_chr22_enriched\t280964\t280964\t30410\t138163\t112391\t13319\t6127\t6515\t21540\t98.91\t10770.0\t3063.5\t3257.5" + line: "SRR891268_chr22_enriched\t280964\t280964\t30410\t109333\t141221\t13319\t6127\t6515\t21540\t98.91\t10770.0\t3063.5\t3257.5" cutadapt: asserts: has_line: - line: "SRR891268_chr22_enriched_2 4.0 285247 41011 40415 4283 280964 28524700 480633 27163516 4.771948521807416" + line: "SRR891268_chr22_enriched_2\t4.7\t285247\t41011\t40415\t4283\t280964\t28524700\t480633\t27163516\t4.771948521807416" general_stats: asserts: has_text: - text: "SRR891268_chr22_enriched 200.0 255 0.028469 98.91" + text: "SRR891268_chr22_enriched\t200.0\t223\t0.028469\t98.91" macs: asserts: has_line: - line: "SRR891268_chr22_enriched 255 47.0 239626.0 200.0" + line: "SRR891268_chr22_enriched\t223\t47.0\t232804.0\t200.0" picard_dups: asserts: has_line: - line: "SRR891268_chr22_enriched Unknown Library 0.0 119813.0 0.0 0.0 0.0 3411.0 5.0 0.028469 2067029.0" + line: "SRR891268_chr22_enriched\tUnknown Library\t0.0\t119813.0\t0.0\t0.0\t0.0\t3411.0\t5.0\t0.028469\t2067029.0" sources: asserts: has_n_lines: diff --git a/workflows/epigenetics/atacseq/atacseq.ga b/workflows/epigenetics/atacseq/atacseq.ga index 1f536db7b..311bd270f 100644 --- a/workflows/epigenetics/atacseq/atacseq.ga +++ b/workflows/epigenetics/atacseq/atacseq.ga @@ -1,6 +1,6 @@ { "a_galaxy_workflow": "true", - "annotation": "This workflow take as input a collection of paired fastq. It will remove bad quality and adapters with cutadapt. Map with Bowtie2 end-to-end. Will remove reads on MT and unconcordant pairs and pairs with mapping quality below 30 and PCR duplicates. Will compute the pile-up on 5' +- 100bp. Will call peaks and count the number of reads falling in the 1kb region centered on the summit. Will plot the number of reads for each fragment length.", + "annotation": "This workflow takes as input a collection of paired fastq. It will remove bad quality and adapters with cutadapt. Map with Bowtie2 end-to-end. Will remove reads on MT and unconcordant pairs and pairs with mapping quality below 30 and PCR duplicates. Will compute the pile-up on 5' +- 100bp. Will call peaks and count the number of reads falling in the 1kb region centered on the summit. Will compute 2 normalization for coverage: normalized by million reads and normalized by million reads in peaks. Will plot the number of reads for each fragment length.", "creator": [ { "class": "Person", @@ -10,7 +10,7 @@ ], "format-version": "0.1", "license": "MIT", - "release": "0.1", + "release": "0.13", "name": "ATACseq", "steps": { "0": { @@ -37,6 +37,7 @@ "tool_version": null, "type": "data_collection_input", "uuid": "3b1d869d-e8a9-49d9-894c-8aa500cd445d", + "when": null, "workflow_outputs": [] }, "1": { @@ -63,6 +64,7 @@ "tool_version": null, "type": "parameter_input", "uuid": "c085055c-9d18-49d7-9f87-fae61683d775", + "when": null, "workflow_outputs": [] }, "2": { @@ -89,20 +91,53 @@ "tool_version": null, "type": "parameter_input", "uuid": "43f5c231-ed56-4cda-bd50-0495dfd77d70", + "when": null, "workflow_outputs": [] }, "3": { - "annotation": "", - "content_id": "toolshed.g2.bx.psu.edu/repos/lparsons/cutadapt/cutadapt/4.0+galaxy0", + "annotation": "Bin size for normalized bigwig (usually 50bp is sufficient)", + "content_id": null, "errors": null, "id": 3, + "input_connections": {}, + "inputs": [ + { + "description": "Bin size for normalized bigwig (usually 50bp is sufficient)", + "name": "bin_size" + } + ], + "label": "bin_size", + "name": "Input parameter", + "outputs": [], + "position": { + "left": 155.54247082439377, + "top": 898.8496207394044 + }, + "tool_id": null, + "tool_state": "{\"parameter_type\": \"integer\", \"optional\": false}", + "tool_version": null, + "type": "parameter_input", + "uuid": "942c77e6-f462-49ac-87ed-6e4e14589b0b", + "when": null, + "workflow_outputs": [] + }, + "4": { + "annotation": "", + "content_id": "toolshed.g2.bx.psu.edu/repos/lparsons/cutadapt/cutadapt/4.7+galaxy0", + "errors": null, + "id": 4, "input_connections": { "library|input_1": { "id": 0, "output_name": "output" } }, - 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{ - "label": "MultiQC on input dataset(s): Stats", - "output_name": "stats", - "uuid": "6071150d-48db-4cf8-bfed-2cbdb2635856" - }, { "label": "MultiQC webpage", "output_name": "html_report", "uuid": "c22aafb2-d9f2-43c3-a6c4-cfe4a3166c07" + }, + { + "label": "MultiQC on input dataset(s): Stats", + "output_name": "stats", + "uuid": "6071150d-48db-4cf8-bfed-2cbdb2635856" } ] } @@ -1232,6 +1664,6 @@ "tags": [ "ATACseq" ], - "uuid": "eb8e9537-3716-43ea-8e07-afb56a7865cb", - "version": 25 + "uuid": "02785f0c-c445-4419-9f95-a202eaf6493a", + "version": 1 } \ No newline at end of file diff --git a/workflows/epigenetics/average-bigwig-between-replicates/.dockstore.yml b/workflows/epigenetics/average-bigwig-between-replicates/.dockstore.yml new file mode 100644 index 000000000..a58e6582b --- /dev/null +++ b/workflows/epigenetics/average-bigwig-between-replicates/.dockstore.yml @@ -0,0 +1,11 @@ +version: 1.2 +workflows: +- name: main + subclass: Galaxy + publish: true + primaryDescriptorPath: /average-bigwig-between-replicates.ga + testParameterFiles: + - /average-bigwig-between-replicates-tests.yml + authors: + - name: Lucille Delisle + orcid: 0000-0002-1964-4960 diff --git a/workflows/epigenetics/average-bigwig-between-replicates/.workflowhub.yml b/workflows/epigenetics/average-bigwig-between-replicates/.workflowhub.yml new file mode 100644 index 000000000..d423b9bc6 --- /dev/null +++ b/workflows/epigenetics/average-bigwig-between-replicates/.workflowhub.yml @@ -0,0 +1,5 @@ +version: '0.1' +registries: +- url: https://workflowhub.eu + project: iwc + workflow: average-bigwig-between-replicates-tests/main diff --git a/workflows/epigenetics/average-bigwig-between-replicates/CHANGELOG.md b/workflows/epigenetics/average-bigwig-between-replicates/CHANGELOG.md new file mode 100644 index 000000000..d7a185ed5 --- /dev/null +++ b/workflows/epigenetics/average-bigwig-between-replicates/CHANGELOG.md @@ -0,0 +1,9 @@ +# Changelog + +## [0.2] 2023-09-27 + +### Automatic update +- `toolshed.g2.bx.psu.edu/repos/bgruening/deeptools_bigwig_average/deeptools_bigwig_average/3.5.2+galaxy0` was updated to `toolshed.g2.bx.psu.edu/repos/bgruening/deeptools_bigwig_average/deeptools_bigwig_average/3.5.4+galaxy0` + +## [0.1] 2023-09-22 +First release. diff --git a/workflows/epigenetics/average-bigwig-between-replicates/README.md b/workflows/epigenetics/average-bigwig-between-replicates/README.md new file mode 100644 index 000000000..59aed0818 --- /dev/null +++ b/workflows/epigenetics/average-bigwig-between-replicates/README.md @@ -0,0 +1,38 @@ +# Average Bigwig between replicates + +This workflow is very useful when you processed multiple samples in collections and you want to generate an average coverage per condition. + +## Inputs dataset + +- The workflow needs a single input which is a list of bigwigs (normalized). The identifiers of your bigwigs must be like: + - whatever_sample1_identificationOfReplicate1 + - whatever_sample1_identificationOfReplicate2 + - ... + - whatever_sample2_identificationOfReplicate1 + - whatever_sample2_identificationOfReplicate2 + - ... + +## Inputs values + +- bin_size: this is used when average of coverage is performed. Large values will allow to have smaller output files but with less resolution while small values will increase computation time and size of output files to produce more resolutive bigwigs. I suggest 5bp for RNA-seq and 50bp for other applications. + +## Processing + +- The workflow will split identifiers between everything which is before the last underscore which will be the *sample* and everything which is after the last underscore which will be the *replicate identifier*. And restructure the collection as list:list: + - whatever_sample1: + - identificationOfReplicate1 + - identificationOfReplicate2 + - ... + - whatever_sample2: + - identificationOfReplicate1 + - identificationOfReplicate2 + - --- + - ... +- Then it will average bigwigs into each inner list + +## Outputs + +- The output is a collection of bigwig datasets like: + - whatever_sample1 + - whatever_sample2 + - ... diff --git a/workflows/epigenetics/average-bigwig-between-replicates/average-bigwig-between-replicates-tests.yml b/workflows/epigenetics/average-bigwig-between-replicates/average-bigwig-between-replicates-tests.yml new file mode 100644 index 000000000..3f21209d2 --- /dev/null +++ b/workflows/epigenetics/average-bigwig-between-replicates/average-bigwig-between-replicates-tests.yml @@ -0,0 +1,38 @@ +- doc: Test outline for average-bigwig-between-replicates.ga + job: + Bigwig to average: + class: Collection + collection_type: list + elements: + - identifier: ATAC_HH19_PT_rep1 + class: File + location: https://www.ncbi.nlm.nih.gov/geo/download/?acc=GSM6152756&format=file&file=GSM6152756%5FATAC%5FHH19%5FPT%5Frep1%2Ebigwig + filetype: bigwig + - identifier: ATAC_HH19_PT_rep2 + class: File + location: https://www.ncbi.nlm.nih.gov/geo/download/?acc=GSM6152757&format=file&file=GSM6152757%5FATAC%5FHH19%5FPT%5Frep2%2Ebigwig + filetype: bigwig + - identifier: ATAC_HH35_DS_rep1 + class: File + location: https://www.ncbi.nlm.nih.gov/geo/download/?acc=GSM6152758&format=file&file=GSM6152758%5FATAC%5FHH35%5FDS%5Frep1%2Ebigwig + filetype: bigwig + - identifier: ATAC_HH35_DS_rep2 + class: File + location: https://www.ncbi.nlm.nih.gov/geo/download/?acc=GSM6152759&format=file&file=GSM6152759%5FATAC%5FHH35%5FDS%5Frep2%2Ebigwig + filetype: bigwig + bin_size: 50 + outputs: + average_bigwigs: + class: Collection + collection_type: list + element_tests: + ATAC_HH19_PT: + asserts: + has_size: + value: 140203524 + delta: 10000000 + ATAC_HH35_DS: + asserts: + has_size: + value: 124599299 + delta: 10000000 diff --git a/workflows/epigenetics/average-bigwig-between-replicates/average-bigwig-between-replicates.ga b/workflows/epigenetics/average-bigwig-between-replicates/average-bigwig-between-replicates.ga new file mode 100644 index 000000000..1e5edfc2f --- /dev/null +++ b/workflows/epigenetics/average-bigwig-between-replicates/average-bigwig-between-replicates.ga @@ -0,0 +1,167 @@ +{ + "a_galaxy_workflow": "true", + "annotation": "We assume the identifiers of the input list are like:\nsample_name_replicateID.\nThe identifiers of the output list will be:\nsample_name", + "creator": [ + { + "class": "Person", + "identifier": "https://orcid.org/0000-0002-1964-4960", + "name": "Lucille Delisle" + } + ], + "format-version": "0.1", + "license": "MIT", + "release": "0.2", + "name": "Average Bigwig between replicates", + "steps": { + "0": { + "annotation": "We assume the identifiers are like:\nsample_name_replicateID", + "content_id": null, + "errors": null, + "id": 0, + "input_connections": {}, + "inputs": [ + { + "description": "We assume the identifiers are like:\nsample_name_replicateID", + "name": "Bigwig to average" + } + ], + "label": "Bigwig to average", + "name": "Input dataset collection", + "outputs": [], + "position": { + "left": 0, + "top": 0.0 + }, + "tool_id": null, + "tool_state": "{\"optional\": false, \"tag\": \"\", \"collection_type\": \"list\"}", + "tool_version": null, + "type": "data_collection_input", + "uuid": "45d1d287-ea71-4817-afd2-8ad89aedac46", + "when": null, + "workflow_outputs": [] + }, + "1": { + "annotation": "Bin size for normalized bigwig (usually 5bp is sufficient for RNA-seq and 50bp for ChIP/CUTandRUN/ATAC)", + "content_id": null, + "errors": null, + "id": 1, + "input_connections": {}, + "inputs": [ + { + "description": "Bin size for normalized bigwig (usually 5bp is sufficient for RNA-seq and 50bp for ChIP/CUTandRUN/ATAC)", + "name": "bin_size" + } + ], + "label": "bin_size", + "name": "Input parameter", + "outputs": [], + "position": { + "left": 16, + "top": 125.0 + }, + "tool_id": null, + "tool_state": "{\"parameter_type\": \"integer\", \"optional\": false}", + "tool_version": null, + "type": "parameter_input", + "uuid": "ae41572a-bafb-40d4-8042-87f07552980f", + "when": null, + "workflow_outputs": [] + }, + "2": { + "annotation": "", + "content_id": "__APPLY_RULES__", + "errors": null, + "id": 2, + "input_connections": { + "input": { + "id": 0, + "output_name": "output" + } + }, + "inputs": [], + "label": null, + "name": "Apply rules", + "outputs": [ + { + "name": "output", + "type": "input" + } + ], + "position": { + "left": 254, + "top": 11.5 + }, + "post_job_actions": { + "HideDatasetActionoutput": { + "action_arguments": {}, + "action_type": "HideDatasetAction", + "output_name": "output" + } + }, + "tool_id": "__APPLY_RULES__", + "tool_state": "{\"input\": {\"__class__\": \"ConnectedValue\"}, \"rules\": {\"mapping\": [{\"collapsible_value\": {\"__class__\": \"RuntimeValue\"}, 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"bgruening", + "tool_shed": "toolshed.g2.bx.psu.edu" + }, + "tool_state": "{\"advancedOpt\": {\"showAdvancedOpt\": \"yes\", \"__current_case__\": 1, \"binSize\": {\"__class__\": \"ConnectedValue\"}, \"skipNAs\": false, \"scaleFactors\": \"1\", \"blackListFileName\": {\"__class__\": \"RuntimeValue\"}}, \"bigwigs\": {\"__class__\": \"ConnectedValue\"}, \"outFileFormat\": \"bigwig\", \"region\": \"\", \"__page__\": null, \"__rerun_remap_job_id__\": null}", + "tool_version": "3.5.4+galaxy0", + "type": "tool", + "uuid": "f63daf4e-ce9f-4301-be74-e4c263b6e88b", + "when": null, + "workflow_outputs": [ + { + "label": "average_bigwigs", + "output_name": "outFileName", + "uuid": "19023604-eee1-4099-b1c2-abe3de93b3f3" + } + ] + } + }, + "tags": [], + "uuid": "c6ed4b0e-d719-4997-9860-ba99488ec332", + "version": 6 +} \ No newline at end of file diff --git a/workflows/epigenetics/chipseq-pe/.dockstore.yml b/workflows/epigenetics/chipseq-pe/.dockstore.yml index c63f72bf4..138f3c0d4 100644 --- a/workflows/epigenetics/chipseq-pe/.dockstore.yml +++ b/workflows/epigenetics/chipseq-pe/.dockstore.yml @@ -1,8 +1,11 @@ version: 1.2 workflows: - name: main - primaryDescriptorPath: /chipseq-pe.ga - publish: true subclass: Galaxy + publish: true + primaryDescriptorPath: /chipseq-pe.ga testParameterFiles: - /chipseq-pe-tests.yml + authors: + - name: Lucille Delisle + orcid: 0000-0002-1964-4960 diff --git a/workflows/epigenetics/chipseq-pe/CHANGELOG.md b/workflows/epigenetics/chipseq-pe/CHANGELOG.md index 1fa96f031..733425f84 100644 --- a/workflows/epigenetics/chipseq-pe/CHANGELOG.md +++ b/workflows/epigenetics/chipseq-pe/CHANGELOG.md @@ -1,5 +1,50 @@ # Changelog +## [0.8] 2024-04-08 + +### Automatic update +- `toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_grep_tool/9.3+galaxy0` was updated to `toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_grep_tool/9.3+galaxy1` + +## [0.7] 2024-03-25 + +### Automatic update +- `toolshed.g2.bx.psu.edu/repos/lparsons/cutadapt/cutadapt/4.6+galaxy1` was updated to `toolshed.g2.bx.psu.edu/repos/lparsons/cutadapt/cutadapt/4.7+galaxy0` + +## [0.6.1] 2024-03-14 + +Fix bug introduced in 0.6: the second adapter sequence was not used. + +## [0.6] 2024-02-05 + +### Automatic update +- `toolshed.g2.bx.psu.edu/repos/lparsons/cutadapt/cutadapt/4.4+galaxy0` was updated to `toolshed.g2.bx.psu.edu/repos/lparsons/cutadapt/cutadapt/4.6+galaxy1` +- `toolshed.g2.bx.psu.edu/repos/devteam/bowtie2/bowtie2/2.5.0+galaxy0` was updated to `toolshed.g2.bx.psu.edu/repos/devteam/bowtie2/bowtie2/2.5.3+galaxy0` +- `toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_grep_tool/1.1.1` was updated to `toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_grep_tool/9.3+galaxy0` + +## [0.5] 2023-10-17 + +### Automatic update +- `toolshed.g2.bx.psu.edu/repos/iuc/macs2/macs2_callpeak/2.2.7.1+galaxy0` was updated to `toolshed.g2.bx.psu.edu/repos/iuc/macs2/macs2_callpeak/2.2.9.1+galaxy0` + +## [0.4] 2023-06-17 + +### Automatic update +- `toolshed.g2.bx.psu.edu/repos/lparsons/cutadapt/cutadapt/4.0+galaxy1` was updated to `toolshed.g2.bx.psu.edu/repos/lparsons/cutadapt/cutadapt/4.4+galaxy0` + +### Manual update +- New parameter to get normalized profile + +## [0.3] 2022-12-17 + +### Automatic update +- `toolshed.g2.bx.psu.edu/repos/iuc/multiqc/multiqc/1.11+galaxy0` was updated to `toolshed.g2.bx.psu.edu/repos/iuc/multiqc/multiqc/1.11+galaxy1` + +## [0.2] 2022-11-28 + +### Automatic update +- `toolshed.g2.bx.psu.edu/repos/lparsons/cutadapt/cutadapt/4.0+galaxy0` was updated to `toolshed.g2.bx.psu.edu/repos/lparsons/cutadapt/cutadapt/4.0+galaxy1` +- `toolshed.g2.bx.psu.edu/repos/devteam/bowtie2/bowtie2/2.4.5+galaxy1` was updated to `toolshed.g2.bx.psu.edu/repos/devteam/bowtie2/bowtie2/2.5.0+galaxy0` + ## 2022-10-20 ChIPseq_PE has been renamed chipseq-pe (still version 0.1) diff --git a/workflows/epigenetics/chipseq-pe/README.md b/workflows/epigenetics/chipseq-pe/README.md index b30f5656c..b41f1805d 100644 --- a/workflows/epigenetics/chipseq-pe/README.md +++ b/workflows/epigenetics/chipseq-pe/README.md @@ -9,19 +9,19 @@ - adapters sequences: this depends on the library preparation. If you don't know, use FastQC to determine if it is Truseq or Nextera. - reference_genome: this field will be adapted to the genomes available for bowtie2. - effective_genome_size: this is used by MACS2 and may be entered manually (indications are provided for heavily used genomes). +- normalize_profile: Whether you want to have a profile normalized as Signal to Million Fragments. ## Processing - The workflow will remove illumina adapters and low quality bases and filter out any pair with mate smaller than 15bp. - The filtered reads are mapped with bowtie2 with default parameters. - The BAM is filtered to keep only MAPQ30 and concordant pairs. -- The peaks are called with MACS2 which at the same time generates a coverage file. +- The peaks are called with MACS2 which at the same time generates a coverage file (normalized or not). - The coverage is converted to bigwig. - A MultiQC is run to have an overview of the QC. ### Warning -- The coverage output is not normalized. - The filtered bam still has PCR duplicates which are removed by MACS2. ## Contribution diff --git a/workflows/epigenetics/chipseq-pe/chipseq-pe-tests.yml b/workflows/epigenetics/chipseq-pe/chipseq-pe-tests.yml index 7aa933c24..aba2de776 100644 --- a/workflows/epigenetics/chipseq-pe/chipseq-pe-tests.yml +++ b/workflows/epigenetics/chipseq-pe/chipseq-pe-tests.yml @@ -20,6 +20,7 @@ adapter_reverse: 'GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT' reference_genome: 'mm10' effective_genome_size: 1870000000 + normalize_profile: true outputs: MultiQC webpage: asserts: @@ -37,11 +38,11 @@ text: "46307\t46307\t1292" cutadapt: asserts: - has_text: - text: "4.0\t50000\t587\t550\t3693\t46307\t5100000" + has_text: + text: "4.7\t50000\t587\t550\t3693\t46307\t5100000" general_stats: asserts: - has_text: + has_text: text: "201.0\t0.0\t11\t98.68" macs: asserts: @@ -95,7 +96,7 @@ wt_H3K4me3: asserts: has_size: - value: 527913 + value: 556477 delta: 10000 mapping stats: element_tests: @@ -104,6 +105,6 @@ - that: "has_text" text: "1292 (2.79%) aligned concordantly 0 times" - that: "has_text" - text: "41729 (90.11%) aligned concordantly exactly 1 time" + text: "39329 (84.93%) aligned concordantly exactly 1 time" - that: "has_text" - text: "3286 (7.10%) aligned concordantly >1 times" + text: "5686 (12.28%) aligned concordantly >1 times" diff --git a/workflows/epigenetics/chipseq-pe/chipseq-pe.ga b/workflows/epigenetics/chipseq-pe/chipseq-pe.ga index 16e3b365f..4f1d73ee3 100644 --- a/workflows/epigenetics/chipseq-pe/chipseq-pe.ga +++ b/workflows/epigenetics/chipseq-pe/chipseq-pe.ga @@ -10,7 +10,7 @@ ], "format-version": "0.1", "license": "MIT", - "release": "0.1", + "release": "0.8", "name": "ChIPseq_PE", "steps": { "0": { @@ -37,6 +37,7 @@ "tool_version": null, "type": "data_collection_input", "uuid": "e09c0852-1db3-4a68-b88c-1b94c205cb6c", + "when": null, "workflow_outputs": [] }, "1": { @@ -55,14 +56,15 @@ "name": "Input parameter", "outputs": [], "position": { - "left": 31.01666259765625, - "top": 81.5 + "left": 11.01666259765625, + "top": 93.5 }, "tool_id": null, "tool_state": "{\"parameter_type\": \"text\", \"optional\": false}", "tool_version": null, "type": "parameter_input", "uuid": "30c1d867-5e73-4348-8969-848f58d94015", + "when": null, "workflow_outputs": [] }, "2": { @@ -81,14 +83,15 @@ "name": "Input parameter", "outputs": [], "position": { - "left": 72.066650390625, - "top": 173.4499969482422 + "left": 50.066650390625, + "top": 189.45001220703125 }, "tool_id": null, "tool_state": "{\"parameter_type\": \"text\", \"optional\": false}", "tool_version": null, "type": "parameter_input", "uuid": "ec244ede-8fa3-4d05-85a0-06839f4cf97d", + "when": null, "workflow_outputs": [] }, "3": { @@ -107,14 +110,15 @@ "name": "Input parameter", "outputs": [], "position": { - "left": 177.9000244140625, - "top": 305.23333740234375 + "left": 102.9000244140625, + "top": 333.23333740234375 }, "tool_id": null, "tool_state": "{\"restrictOnConnections\": true, \"parameter_type\": \"text\", \"optional\": false}", "tool_version": null, "type": "parameter_input", "uuid": "0119d669-7ce9-46fd-ba6f-3efd92dfb7f2", + "when": null, "workflow_outputs": [] }, "4": { @@ -133,21 +137,49 @@ "name": "Input parameter", "outputs": [], "position": { - "left": 235.8499755859375, - "top": 399.6166687011719 + "left": 144.8499755859375, + "top": 437.61669921875 }, "tool_id": null, "tool_state": "{\"parameter_type\": \"integer\", \"optional\": false}", "tool_version": null, "type": "parameter_input", "uuid": "5bb3b3df-60ab-4ec7-88e4-476be547ffbf", + "when": null, "workflow_outputs": [] }, "5": { - "annotation": "", - "content_id": "toolshed.g2.bx.psu.edu/repos/lparsons/cutadapt/cutadapt/4.0+galaxy0", + "annotation": "Whether you want to have a profile normalized as Signal to Million Fragments", + "content_id": null, "errors": null, "id": 5, + "input_connections": {}, + "inputs": [ + { + "description": "Whether you want to have a profile normalized as Signal to Million Fragments", + "name": "normalize_profile" + } + ], + "label": "normalize_profile", + "name": "Input parameter", + "outputs": [], + "position": { + "left": 185, + "top": 542 + }, + "tool_id": null, + "tool_state": "{\"parameter_type\": \"boolean\", \"optional\": false}", + "tool_version": null, + "type": "parameter_input", + "uuid": "8180a47b-2fa4-404e-b644-1f3ec3bea980", + "when": null, + "workflow_outputs": [] + }, + "6": { + "annotation": "", + "content_id": "toolshed.g2.bx.psu.edu/repos/lparsons/cutadapt/cutadapt/4.7+galaxy0", + "errors": null, + "id": 6, "input_connections": { "library|input_1": { "id": 0, @@ -157,7 +189,7 @@ "id": 1, "output_name": "output" }, - 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"id": 10, + "id": 11, "input_connections": { "input1": { - "id": 8, + "id": 9, "output_name": "output_treat_pileup" } }, - "inputs": [ - { - "description": "runtime parameter for tool Wig/BedGraph-to-bigWig", - "name": "input1" - } - ], + "inputs": [], "label": "Bigwig from MACS2", "name": "Wig/BedGraph-to-bigWig", "outputs": [ @@ -578,8 +617,8 @@ } ], "position": { - "left": 1929.933349609375, - "top": 411.3333282470703 + "left": 2017.933349609375, + "top": 464.3333282470703 }, "post_job_actions": { "RenameDatasetActionout_file1": { @@ -591,10 +630,11 @@ } }, "tool_id": "wig_to_bigWig", - "tool_state": "{\"input1\": {\"__class__\": \"RuntimeValue\"}, \"settings\": {\"settingsType\": \"preset\", \"__current_case__\": 0}, \"__page__\": null, \"__rerun_remap_job_id__\": null}", + "tool_state": "{\"input1\": {\"__class__\": \"ConnectedValue\"}, \"settings\": {\"settingsType\": \"preset\", \"__current_case__\": 0}, \"__page__\": null, \"__rerun_remap_job_id__\": null}", "tool_version": "1.1.1", "type": "tool", "uuid": "0fe57c5d-00a0-4cb6-9bac-97e6d03c6b76", + "when": null, "workflow_outputs": [ { "label": "coverage from MACS2", @@ -603,22 +643,22 @@ } ] }, - "11": { + "12": { "annotation": "", - "content_id": "toolshed.g2.bx.psu.edu/repos/iuc/multiqc/multiqc/1.11+galaxy0", + "content_id": "toolshed.g2.bx.psu.edu/repos/iuc/multiqc/multiqc/1.11+galaxy1", "errors": null, - "id": 11, + "id": 12, "input_connections": { "results_0|software_cond|input": { - "id": 5, + "id": 6, "output_name": "report" }, "results_1|software_cond|input": { - "id": 6, + "id": 7, "output_name": "mapping_stats" }, "results_2|software_cond|input": { - "id": 8, + "id": 9, "output_name": "output_tabular" } }, @@ -640,8 +680,8 @@ } ], "position": { - "left": 1977.1666870117188, - "top": 790.3333129882812 + "left": 2065.1666870117188, + "top": 843.3333129882812 }, "post_job_actions": { "HideDatasetActionplots": { @@ -650,27 +690,28 @@ "output_name": "plots" } }, - "tool_id": "toolshed.g2.bx.psu.edu/repos/iuc/multiqc/multiqc/1.11+galaxy0", + "tool_id": "toolshed.g2.bx.psu.edu/repos/iuc/multiqc/multiqc/1.11+galaxy1", "tool_shed_repository": { - "changeset_revision": "9a913cdee30e", + "changeset_revision": "abfd8a6544d7", "name": "multiqc", "owner": "iuc", "tool_shed": "toolshed.g2.bx.psu.edu" }, - "tool_state": "{\"comment\": \"\", \"export\": \"true\", \"flat\": \"false\", \"results\": [{\"__index__\": 0, \"software_cond\": {\"software\": \"cutadapt\", \"__current_case__\": 5, \"input\": {\"__class__\": \"RuntimeValue\"}}}, {\"__index__\": 1, \"software_cond\": {\"software\": \"bowtie2\", \"__current_case__\": 3, \"input\": {\"__class__\": \"RuntimeValue\"}}}, {\"__index__\": 2, \"software_cond\": {\"software\": \"macs2\", \"__current_case__\": 16, \"input\": {\"__class__\": \"RuntimeValue\"}}}], \"saveLog\": \"false\", \"title\": \"\", \"__page__\": null, \"__rerun_remap_job_id__\": null}", - "tool_version": "1.11+galaxy0", + "tool_state": "{\"comment\": \"\", \"export\": true, \"flat\": false, \"results\": [{\"__index__\": 0, \"software_cond\": {\"software\": \"cutadapt\", \"__current_case__\": 5, \"input\": {\"__class__\": \"ConnectedValue\"}}}, {\"__index__\": 1, \"software_cond\": {\"software\": \"bowtie2\", \"__current_case__\": 3, \"input\": {\"__class__\": \"ConnectedValue\"}}}, {\"__index__\": 2, \"software_cond\": {\"software\": \"macs2\", \"__current_case__\": 16, \"input\": {\"__class__\": \"ConnectedValue\"}}}], \"saveLog\": false, \"title\": \"\", \"__page__\": null, \"__rerun_remap_job_id__\": null}", + "tool_version": "1.11+galaxy1", "type": "tool", "uuid": "fb066848-43df-412a-9767-9613bac7d961", + "when": null, "workflow_outputs": [ - { - "label": "MultiQC on input dataset(s): Stats", - "output_name": "stats", - "uuid": "409a12c8-c81d-4607-a7d8-3f8fe0ab8d02" - }, { "label": "MultiQC webpage", "output_name": "html_report", "uuid": "175c983c-cb66-430e-aaeb-252b9f520f3b" + }, + { + "label": "MultiQC on input dataset(s): Stats", + "output_name": "stats", + "uuid": "409a12c8-c81d-4607-a7d8-3f8fe0ab8d02" } ] } @@ -678,6 +719,6 @@ "tags": [ "ChIP" ], - "uuid": "12593cdc-9a1d-4cf3-810f-edeeee35b9d7", + "uuid": "1834d09c-c683-4424-bc8d-57df156c2fc8", "version": 1 } \ No newline at end of file diff --git a/workflows/epigenetics/chipseq-sr/.dockstore.yml b/workflows/epigenetics/chipseq-sr/.dockstore.yml index 9d0c4bb29..05b3726bf 100644 --- a/workflows/epigenetics/chipseq-sr/.dockstore.yml +++ b/workflows/epigenetics/chipseq-sr/.dockstore.yml @@ -1,8 +1,11 @@ version: 1.2 workflows: - name: main - primaryDescriptorPath: /chipseq-sr.ga - publish: true subclass: Galaxy + publish: true + primaryDescriptorPath: /chipseq-sr.ga testParameterFiles: - /chipseq-sr-tests.yml + authors: + - name: Lucille Delisle + orcid: 0000-0002-1964-4960 diff --git a/workflows/epigenetics/chipseq-sr/CHANGELOG.md b/workflows/epigenetics/chipseq-sr/CHANGELOG.md index 74b4bbfef..f99d544ad 100644 --- a/workflows/epigenetics/chipseq-sr/CHANGELOG.md +++ b/workflows/epigenetics/chipseq-sr/CHANGELOG.md @@ -1,4 +1,45 @@ # Changelog +## [0.8] 2024-04-08 + +### Automatic update +- `toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_grep_tool/9.3+galaxy0` was updated to `toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_grep_tool/9.3+galaxy1` + +## [0.7] 2024-03-25 + +### Automatic update +- `toolshed.g2.bx.psu.edu/repos/lparsons/cutadapt/cutadapt/4.6+galaxy1` was updated to `toolshed.g2.bx.psu.edu/repos/lparsons/cutadapt/cutadapt/4.7+galaxy0` + +## [0.6] 2024-02-05 + +### Automatic update +- `toolshed.g2.bx.psu.edu/repos/lparsons/cutadapt/cutadapt/4.4+galaxy0` was updated to `toolshed.g2.bx.psu.edu/repos/lparsons/cutadapt/cutadapt/4.6+galaxy1` +- `toolshed.g2.bx.psu.edu/repos/devteam/bowtie2/bowtie2/2.5.0+galaxy0` was updated to `toolshed.g2.bx.psu.edu/repos/devteam/bowtie2/bowtie2/2.5.3+galaxy0` +- `toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_grep_tool/1.1.1` was updated to `toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_grep_tool/9.3+galaxy0` + +## [0.5] 2023-10-17 + +### Automatic update +- `toolshed.g2.bx.psu.edu/repos/iuc/macs2/macs2_callpeak/2.2.7.1+galaxy0` was updated to `toolshed.g2.bx.psu.edu/repos/iuc/macs2/macs2_callpeak/2.2.9.1+galaxy0` + +## [0.4] 2023-06-17 + +### Automatic update +- `toolshed.g2.bx.psu.edu/repos/lparsons/cutadapt/cutadapt/4.0+galaxy1` was updated to `toolshed.g2.bx.psu.edu/repos/lparsons/cutadapt/cutadapt/4.4+galaxy0` + +### Manual update +- New parameter to get normalized profile + +## [0.3] 2022-12-17 + +### Automatic update +- `toolshed.g2.bx.psu.edu/repos/iuc/multiqc/multiqc/1.11+galaxy0` was updated to `toolshed.g2.bx.psu.edu/repos/iuc/multiqc/multiqc/1.11+galaxy1` + +## [0.2] 2022-11-28 + +### Automatic update +- `toolshed.g2.bx.psu.edu/repos/lparsons/cutadapt/cutadapt/4.0+galaxy0` was updated to `toolshed.g2.bx.psu.edu/repos/lparsons/cutadapt/cutadapt/4.0+galaxy1` +- `toolshed.g2.bx.psu.edu/repos/devteam/bowtie2/bowtie2/2.4.5+galaxy1` was updated to `toolshed.g2.bx.psu.edu/repos/devteam/bowtie2/bowtie2/2.5.0+galaxy0` + ## [0.1] 2022-10-18 First release. diff --git a/workflows/epigenetics/chipseq-sr/README.md b/workflows/epigenetics/chipseq-sr/README.md index 9d97a32aa..0591c9d91 100644 --- a/workflows/epigenetics/chipseq-sr/README.md +++ b/workflows/epigenetics/chipseq-sr/README.md @@ -9,17 +9,17 @@ - adapters sequence_forward: this depends on the library preparation. If you don't know, use FastQC to determine if it is Truseq or Nextera. - reference_genome: this field will be adapted to the genomes available for bowtie2. - effective_genome_size: this is used by MACS2 and may be entered manually (indications are provided for heavily used genomes). +- normalize_profile: Whether you want to have a profile normalized as Signal to Million Reads. ## Processing - The workflow will remove illumina adapters and low quality bases and filter out any read smaller than 15bp. - The filtered reads are mapped with bowtie2 with default parameters. - The BAM is filtered to keep only MAPQ30. -- The peaks are called with MACS2 with a fixed extension of 200bp which at the same time generates a coverage file. +- The peaks are called with MACS2 with a fixed extension of 200bp which at the same time generates a coverage file (normalized or not). - The coverage is converted to bigwig. - A MultiQC is run to have an overview of the QC. ### Warning -- The coverage output is not normalized. - The filtered bam still has PCR duplicates which are removed by MACS2. diff --git a/workflows/epigenetics/chipseq-sr/chipseq-sr-tests.yml b/workflows/epigenetics/chipseq-sr/chipseq-sr-tests.yml index dc56d4dbf..f8b734ff2 100644 --- a/workflows/epigenetics/chipseq-sr/chipseq-sr-tests.yml +++ b/workflows/epigenetics/chipseq-sr/chipseq-sr-tests.yml @@ -11,6 +11,7 @@ adapter_forward: 'GATCGGAAGAGCACACGTCTGAACTCCAGTCAC' reference_genome: 'mm10' effective_genome_size: 1870000000 + normalize_profile: true outputs: MultiQC webpage: asserts: @@ -28,12 +29,12 @@ text: "49251\t49251\t805" cutadapt: asserts: - has_text: - text: "4.0\t50000\t587\t749\t49251" + has_text: + text: "4.7\t50000\t587\t749\t49251" general_stats: asserts: - has_text: - text: "200.0\t0.0\t4\t98.37\t4.612745098039215" + has_text_matching: + expression: "200.0\t0.0\t4\t98.[0-9]*\t4.612745098039215" macs: asserts: has_text: @@ -86,8 +87,8 @@ wt_H3K4me3: asserts: has_size: - value: 527913 - delta: 50000 + value: 559925 + delta: 10000 mapping stats: element_tests: wt_H3K4me3: diff --git a/workflows/epigenetics/chipseq-sr/chipseq-sr.ga b/workflows/epigenetics/chipseq-sr/chipseq-sr.ga index 009d60a5c..24211918f 100644 --- a/workflows/epigenetics/chipseq-sr/chipseq-sr.ga +++ b/workflows/epigenetics/chipseq-sr/chipseq-sr.ga @@ -10,7 +10,7 @@ ], "format-version": "0.1", "license": "MIT", - 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/consensus-peaks-atac-cutandrun-tests.yml + authors: + - name: Lucille Delisle + orcid: 0000-0002-1964-4960 diff --git a/workflows/epigenetics/consensus-peaks/.workflowhub.yml b/workflows/epigenetics/consensus-peaks/.workflowhub.yml new file mode 100644 index 000000000..6f2d3d2ee --- /dev/null +++ b/workflows/epigenetics/consensus-peaks/.workflowhub.yml @@ -0,0 +1,5 @@ +version: '0.1' +registries: +- url: https://workflowhub.eu + project: iwc + workflow: consensus-peaks/consensus-peaks-atac-cutandrun diff --git a/workflows/epigenetics/consensus-peaks/CHANGELOG.md b/workflows/epigenetics/consensus-peaks/CHANGELOG.md new file mode 100644 index 000000000..24e65ed73 --- /dev/null +++ b/workflows/epigenetics/consensus-peaks/CHANGELOG.md @@ -0,0 +1,33 @@ +# Changelog + +## [0.6] 2024-04-08 + +### Automatic update +- `toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_sorted_uniq/9.3+galaxy0` was updated to `toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_sorted_uniq/9.3+galaxy1` +- `toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_sorted_uniq/9.3+galaxy0` was updated to `toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_sorted_uniq/9.3+galaxy1` +- `toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_sorted_uniq/9.3+galaxy0` was updated to `toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_sorted_uniq/9.3+galaxy1` + +## [0.5] 2024-03-05 + +### Automatic update +- `toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_sorted_uniq/1.1.0` was updated to `toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_sorted_uniq/9.3+galaxy0` + +## [0.4] 2023-11-27 + +### Automatic update +- `toolshed.g2.bx.psu.edu/repos/iuc/samtools_view/samtools_view/1.15.1+galaxy0` was updated to `toolshed.g2.bx.psu.edu/repos/iuc/samtools_view/samtools_view/1.15.1+galaxy2` + +## [0.3] 2023-11-27 + +### Automatic update +- `toolshed.g2.bx.psu.edu/repos/iuc/samtools_view/samtools_view/1.15.1+galaxy0` was updated to `toolshed.g2.bx.psu.edu/repos/iuc/samtools_view/samtools_view/1.15.1+galaxy2` + +## [0.2] 2023-11-10 + +### Automatic update +- `toolshed.g2.bx.psu.edu/repos/iuc/macs2/macs2_callpeak/2.2.7.1+galaxy0` was updated to `toolshed.g2.bx.psu.edu/repos/iuc/macs2/macs2_callpeak/2.2.9.1+galaxy0` +- `toolshed.g2.bx.psu.edu/repos/bgruening/deeptools_bigwig_average/deeptools_bigwig_average/3.5.2+galaxy0` was updated to `toolshed.g2.bx.psu.edu/repos/bgruening/deeptools_bigwig_average/deeptools_bigwig_average/3.5.4+galaxy0` +- `toolshed.g2.bx.psu.edu/repos/bgruening/deeptools_bigwig_average/deeptools_bigwig_average/3.5.2+galaxy0` was updated to `toolshed.g2.bx.psu.edu/repos/bgruening/deeptools_bigwig_average/deeptools_bigwig_average/3.5.4+galaxy0` + +## [0.1] 2023-08-31 +First release. diff --git a/workflows/epigenetics/consensus-peaks/README.md b/workflows/epigenetics/consensus-peaks/README.md new file mode 100644 index 000000000..c1efdefbf --- /dev/null +++ b/workflows/epigenetics/consensus-peaks/README.md @@ -0,0 +1,29 @@ +# Consensus peaks Workflow + +The goal of this workflow is to get a list of confident peaks with summits from two replicates. + +## Inputs dataset + +- The workflow needs a single input which is a list of datasets with 2 BAM (or BED) where PCR duplicates have been removed. + +## Inputs values + +- effective_genome_size: this is used by MACS2 and may be entered manually (indications are provided for heavily used genomes). +- bin_size: this is the bin sized used to compute the average of normalized profiles. Large values will allow to have a smaller output file but with less resolution while small values will increase computation time and size of the output file to produce a more resolutive bigwig. + +## Strategy summary + +Here is a generated example to highlight the strategy: +![strategy](./strategy.png) + +## Processing + +- The workflow will: + - first part: + - call peaks and compute normalized coverage on each BAM (or BED) individually + - average normalized profiles + - compute the intersection between both peaks + - second part: + - subset both BAM (or BED) to get the same number of reads + - call peaks on both subsetted BAM combined + - finally, keep only peaks from the second part that have summits overlapping the intersection of the first part. diff --git a/workflows/epigenetics/consensus-peaks/consensus-peaks-atac-cutandrun-tests.yml b/workflows/epigenetics/consensus-peaks/consensus-peaks-atac-cutandrun-tests.yml new file mode 100644 index 000000000..8240cd5b1 --- /dev/null +++ b/workflows/epigenetics/consensus-peaks/consensus-peaks-atac-cutandrun-tests.yml @@ -0,0 +1,52 @@ +- doc: Test for consensus-peaks-atac-cutandrun.ga + job: + 2 rmDup bed: + class: Collection + collection_type: list + elements: + - class: File + identifier: rep1 + path: test-data/rep1.bed + dbkey: mm10 + - class: File + identifier: rep2 + path: test-data/rep2.bed + dbkey: mm10 + effective_genome_size: 1870000000 + bin_size: 50 + outputs: + individual_macs2_narrowPeaks: + element_tests: + rep1: + asserts: + has_n_lines: + n: 4 + delta: 1 + rep2: + asserts: + has_n_lines: + n: 4 + delta: 1 + strict_intersect_peaks: + asserts: + has_n_lines: + n: 4 + delta: 1 + average_bigwig: + asserts: + has_size: + value: 1388 + delta: 100 + merged_macs2_narrowPeaks: + asserts: + has_n_lines: + n: 5 + delta: 1 + multiqc_output: + asserts: + has_text: + text: "Fragment Length" + shared_narrowPeak: + asserts: + has_n_lines: + n: 3 diff --git a/workflows/epigenetics/consensus-peaks/consensus-peaks-atac-cutandrun.ga b/workflows/epigenetics/consensus-peaks/consensus-peaks-atac-cutandrun.ga new file mode 100644 index 000000000..bbfed7633 --- /dev/null +++ b/workflows/epigenetics/consensus-peaks/consensus-peaks-atac-cutandrun.ga @@ -0,0 +1,1085 @@ +{ + "a_galaxy_workflow": "true", + "annotation": "This workflow takes as input BED from ATAC-seq or CUT&RUN. 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<- paste0("read", 1:nrow(frag)) +frag$plus <- "+" +frag$minus <- "-" +frag$score <- 0 +write.table(frag[, c("chr", "start1", "end1", "name", "score", "plus")], + file = "/tmp/sample1_R1.bed", + sep = "\t", col.names = F, row.names = F, quote = F) +write.table(frag[, c("chr", "start2", "end2", "name", "score", "minus")], + file = "/tmp/sample1_R2.bed", + sep = "\t", col.names = F, row.names = F, quote = F) +set.seed(2) +peak.width <- 100 +frag.length <- 350 +read.length <- 40 +gauss.centers <- c(74670050, 74668300, 74665000) +reads.per.peaks <- c(1000, 3000, 1000) +inputs.df <- data.frame(gauss.center = gauss.centers, reads.per.peak = reads.per.peaks) + +rand.center <- do.call(c, apply(inputs.df, 1, function(v){ + rnorm(n = v["reads.per.peak"], mean = v["gauss.center"], sd = peak.width) +})) +rand.frag.length <- rnorm(n = sum(reads.per.peaks), mean = frag.length, sd = frag.length / 4) + +frag <- data.frame(start = as.integer(rand.center - rand.frag.length / 2), + end = as.integer(rand.center + rand.frag.length / 2)) +frag$start1 <- frag$start +frag$end1 <- frag$start + read.length +frag$start2 <- frag$end - read.length +frag$end2 <- frag$end + +frag$end1[frag$end1 > frag$frag$end] <- frag$end[frag$end1 > frag$frag$end] - 1 +frag$start2[frag$start2 < frag$frag$start] <- frag$start[frag$start2 < frag$frag$start] + 1 + +frag$chr <- "chr2" +frag$name <- paste0("read", 1:nrow(frag)) +frag$plus <- "+" +frag$minus <- "-" +frag$score <- 0 +write.table(frag[, c("chr", "start1", "end1", "name", "score", "plus")], + file = "/tmp/sample2_R1.bed", + sep = "\t", col.names = F, row.names = F, quote = F) +write.table(frag[, c("chr", "start2", "end2", "name", "score", "minus")], + file = "/tmp/sample2_R2.bed", + sep = "\t", col.names = F, row.names = F, quote = F) + +# Then in bash +# for sample in 1 2; do for i in 1 2; do bedtools getfasta -nameOnly -s -fi ~/igv/genomes/seq/mm10.fa -bed sample${sample}_R${i}.bed | seqtk seq -F 'A' - > sample${sample}_R${i}.fastq; done; done +# Then in galaxy bowtie2 on mm10 + +# For ATAC/CUT&RUN +# Just BAM to BED (even if it is not really a good way to do it) + +## ChIP-seq SR +set.seed(1) +peak.width <- 100 +frag.length <- 300 +read.length <- 40 +gauss.centers <- c(74670000, 74668000, 74666000) +reads.per.peaks <- c(100, 50, 150) +inputs.df <- data.frame(gauss.center = gauss.centers, reads.per.peak = reads.per.peaks) + +rand.center <- do.call(c, apply(inputs.df, 1, function(v){ + rnorm(n = v["reads.per.peak"], mean = v["gauss.center"], sd = peak.width) +})) +rand.frag.length <- rnorm(n = sum(reads.per.peaks), mean = frag.length, sd = frag.length / 4) + +frag <- data.frame(start = as.integer(rand.center - rand.frag.length / 2), + end = as.integer(rand.center + rand.frag.length / 2)) +frag$start1 <- frag$start +frag$end1 <- frag$start + read.length +frag$start2 <- frag$end - read.length +frag$end2 <- frag$end + +frag$end1[frag$end1 > frag$frag$end] <- frag$end[frag$end1 > frag$frag$end] - 1 +frag$start2[frag$start2 < frag$frag$start] <- frag$start[frag$start2 < frag$frag$start] + 1 + +frag$chr <- "chr2" +frag$name <- paste0("read", 1:nrow(frag)) +frag$score <- 0 +frag$orientation <- sample(c("+", "-"), nrow(frag), replace = TRUE) +frag$read.start <- apply(frag, 1, function(v) { + ifelse(v["orientation"] == "+", v["start1"], v["start2"]) +}) +frag$read.end <- apply(frag, 1, function(v) { + ifelse(v["orientation"] == "+", v["end1"], v["end2"]) +}) +write.table(frag[, c("chr", "read.start", "read.end", "name", "score", "orientation")], + file = "/tmp/rep1.bed", + sep = "\t", col.names = F, row.names = F, quote = F) + + +set.seed(2) +peak.width <- 100 +frag.length <- 350 +read.length <- 40 +gauss.centers <- c(74670050, 74668300, 74665000) +reads.per.peaks <- c(1000, 3000, 1000) +inputs.df <- data.frame(gauss.center = gauss.centers, reads.per.peak = reads.per.peaks) + +rand.center <- do.call(c, apply(inputs.df, 1, function(v){ + rnorm(n = v["reads.per.peak"], mean = v["gauss.center"], sd = peak.width) +})) +rand.frag.length <- rnorm(n = sum(reads.per.peaks), mean = frag.length, sd = frag.length / 4) + +frag <- data.frame(start = as.integer(rand.center - rand.frag.length / 2), + end = as.integer(rand.center + rand.frag.length / 2)) +frag$start1 <- frag$start +frag$end1 <- frag$start + read.length +frag$start2 <- frag$end - read.length +frag$end2 <- frag$end + +frag$end1[frag$end1 > frag$frag$end] <- frag$end[frag$end1 > frag$frag$end] - 1 +frag$start2[frag$start2 < frag$frag$start] <- frag$start[frag$start2 < frag$frag$start] + 1 + +frag$chr <- "chr2" +frag$name <- paste0("read", 1:nrow(frag)) +frag$score <- 0 +frag$orientation <- sample(c("+", "-"), nrow(frag), replace = TRUE) +frag$read.start <- apply(frag, 1, function(v) { + ifelse(v["orientation"] == "+", v["start1"], v["start2"]) +}) +frag$read.end <- apply(frag, 1, function(v) { + ifelse(v["orientation"] == "+", v["end1"], v["end2"]) +}) +write.table(frag[, c("chr", "read.start", "read.end", "name", "score", "orientation")], + file = "/tmp/rep2.bed", + sep = "\t", col.names = F, row.names = F, quote = F) + +# Then in bash +# for sample in 1 2; do bedtools getfasta -nameOnly -s -fi ~/igv/genomes/seq/mm10.fa -bed rep${sample}.bed | seqtk seq -F 'A' - > rep${sample}.fastq; done +# Then in galaxy bowtie2 on mm10 diff --git a/workflows/epigenetics/consensus-peaks/strategy.png b/workflows/epigenetics/consensus-peaks/strategy.png new file mode 100644 index 000000000..55b070d29 Binary files /dev/null and b/workflows/epigenetics/consensus-peaks/strategy.png differ diff --git a/workflows/epigenetics/consensus-peaks/test-data/ChIP_SR_rep1.bam b/workflows/epigenetics/consensus-peaks/test-data/ChIP_SR_rep1.bam new file mode 100644 index 000000000..a138d79cf Binary files /dev/null and b/workflows/epigenetics/consensus-peaks/test-data/ChIP_SR_rep1.bam differ diff --git a/workflows/epigenetics/consensus-peaks/test-data/ChIP_SR_rep2.bam b/workflows/epigenetics/consensus-peaks/test-data/ChIP_SR_rep2.bam new file mode 100644 index 000000000..e5546de2b Binary files /dev/null and b/workflows/epigenetics/consensus-peaks/test-data/ChIP_SR_rep2.bam differ diff --git a/workflows/epigenetics/consensus-peaks/test-data/rep1.bam b/workflows/epigenetics/consensus-peaks/test-data/rep1.bam new file mode 100644 index 000000000..6fb93cbcd Binary files /dev/null and b/workflows/epigenetics/consensus-peaks/test-data/rep1.bam differ diff --git a/workflows/epigenetics/consensus-peaks/test-data/rep1.bed b/workflows/epigenetics/consensus-peaks/test-data/rep1.bed new file mode 100644 index 000000000..158157aab --- /dev/null +++ b/workflows/epigenetics/consensus-peaks/test-data/rep1.bed @@ -0,0 +1,600 @@ +chr2 74665517 74665557 read232(+)/1 42 + +chr2 74665584 74665624 read205(+)/1 42 + +chr2 74665586 74665626 read195(+)/1 42 + +chr2 74665599 74665639 read277(+)/1 42 + +chr2 74665645 74665685 read228(+)/1 42 + +chr2 74665652 74665692 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42 - +chr2 74670401 74670441 read448(-)/2 42 - +chr2 74670404 74670444 read706(-)/2 42 - +chr2 74670408 74670448 read286(-)/2 42 - +chr2 74670410 74670450 read329(-)/2 42 - +chr2 74670410 74670450 read720(-)/2 42 - +chr2 74670412 74670452 read662(-)/2 42 - +chr2 74670414 74670454 read674(-)/2 42 - +chr2 74670417 74670457 read355(-)/2 42 - +chr2 74670423 74670463 read832(-)/2 42 - +chr2 74670423 74670463 read969(-)/2 42 - +chr2 74670425 74670465 read392(-)/2 42 - +chr2 74670429 74670469 read554(-)/2 42 - +chr2 74670430 74670470 read108(-)/2 42 - +chr2 74670435 74670475 read800(-)/2 42 - +chr2 74670438 74670478 read91(-)/2 42 - +chr2 74670446 74670486 read167(-)/2 42 - +chr2 74670447 74670487 read830(-)/2 42 - +chr2 74670453 74670493 read372(-)/2 42 - +chr2 74670457 74670497 read567(-)/2 42 - +chr2 74670462 74670502 read9(-)/2 42 - +chr2 74670462 74670502 read637(-)/2 42 - +chr2 74670468 74670508 read230(-)/2 42 - +chr2 74670474 74670514 read916(-)/2 42 - +chr2 74670478 74670518 read203(-)/2 42 - +chr2 74670480 74670520 read911(-)/2 42 - +chr2 74670482 74670522 read242(-)/2 42 - +chr2 74670485 74670525 read765(-)/2 42 - +chr2 74670486 74670526 read550(-)/2 42 - +chr2 74670503 74670543 read994(-)/2 42 - diff --git a/workflows/epigenetics/cutandrun/.dockstore.yml b/workflows/epigenetics/cutandrun/.dockstore.yml index b710ae18a..32a6f1003 100644 --- a/workflows/epigenetics/cutandrun/.dockstore.yml +++ b/workflows/epigenetics/cutandrun/.dockstore.yml @@ -1,7 +1,11 @@ version: 1.2 workflows: - name: main - primaryDescriptorPath: /cutandrun.ga subclass: Galaxy + publish: true + primaryDescriptorPath: /cutandrun.ga testParameterFiles: - /cutandrun-tests.yml + authors: + - name: Lucille Delisle + orcid: 0000-0002-1964-4960 diff --git a/workflows/epigenetics/cutandrun/CHANGELOG.md b/workflows/epigenetics/cutandrun/CHANGELOG.md index 5e5dd0bc3..f4afe52dc 100644 --- a/workflows/epigenetics/cutandrun/CHANGELOG.md +++ b/workflows/epigenetics/cutandrun/CHANGELOG.md @@ -1,4 +1,56 @@ # Changelog +## [0.9] 2024-04-08 + +### Automatic update +- `toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_grep_tool/9.3+galaxy0` was updated to `toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_grep_tool/9.3+galaxy1` + +## [0.8] 2024-03-25 + +### Automatic update +- `toolshed.g2.bx.psu.edu/repos/lparsons/cutadapt/cutadapt/4.6+galaxy1` was updated to `toolshed.g2.bx.psu.edu/repos/lparsons/cutadapt/cutadapt/4.7+galaxy0` + +## [0.7] 2024-02-05 + +### Automatic update +- `toolshed.g2.bx.psu.edu/repos/lparsons/cutadapt/cutadapt/4.4+galaxy0` was updated to `toolshed.g2.bx.psu.edu/repos/lparsons/cutadapt/cutadapt/4.6+galaxy1` +- `toolshed.g2.bx.psu.edu/repos/devteam/bowtie2/bowtie2/2.5.0+galaxy0` was updated to `toolshed.g2.bx.psu.edu/repos/devteam/bowtie2/bowtie2/2.5.3+galaxy0` +- `toolshed.g2.bx.psu.edu/repos/devteam/picard/picard_MarkDuplicates/2.18.2.4` was updated to `toolshed.g2.bx.psu.edu/repos/devteam/picard/picard_MarkDuplicates/3.1.1.0` +- `toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_grep_tool/1.1.1` was updated to `toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_grep_tool/9.3+galaxy0` + +## [0.6.1] 2023-11-20 + +- Fix author in dockstore + +## [0.6] 2023-10-19 + +Fix the remove duplicate step! +In all previous versions, due to an error, PCR duplicates were not removed. + +## [0.5] 2023-10-17 + +### Automatic update +- `toolshed.g2.bx.psu.edu/repos/iuc/macs2/macs2_callpeak/2.2.7.1+galaxy0` was updated to `toolshed.g2.bx.psu.edu/repos/iuc/macs2/macs2_callpeak/2.2.9.1+galaxy0` + +## [0.4] 2023-03-17 + +### Automatic update +- `toolshed.g2.bx.psu.edu/repos/devteam/picard/picard_MarkDuplicates/2.18.2.3` was updated to `toolshed.g2.bx.psu.edu/repos/devteam/picard/picard_MarkDuplicates/2.18.2.4` +- `toolshed.g2.bx.psu.edu/repos/lparsons/cutadapt/cutadapt/4.0+galaxy1` was updated to `toolshed.g2.bx.psu.edu/repos/lparsons/cutadapt/cutadapt/4.4+galaxy0` + +### Manual update +- New parameter to get normalized profile + +## [0.3] 2022-12-17 + +### Automatic update +- `toolshed.g2.bx.psu.edu/repos/iuc/multiqc/multiqc/1.11+galaxy0` was updated to `toolshed.g2.bx.psu.edu/repos/iuc/multiqc/multiqc/1.11+galaxy1` + +## [0.2] 2022-11-28 + +### Automatic update +- `toolshed.g2.bx.psu.edu/repos/lparsons/cutadapt/cutadapt/4.0+galaxy0` was updated to `toolshed.g2.bx.psu.edu/repos/lparsons/cutadapt/cutadapt/4.0+galaxy1` +- `toolshed.g2.bx.psu.edu/repos/devteam/bowtie2/bowtie2/2.4.5+galaxy1` was updated to `toolshed.g2.bx.psu.edu/repos/devteam/bowtie2/bowtie2/2.5.0+galaxy0` + ## [0.1] 2022-10-06 First release. diff --git a/workflows/epigenetics/cutandrun/README.md b/workflows/epigenetics/cutandrun/README.md index cc9c9c9e7..c122e5dc6 100644 --- a/workflows/epigenetics/cutandrun/README.md +++ b/workflows/epigenetics/cutandrun/README.md @@ -9,18 +9,15 @@ - adapter sequences: this depends on the library preparation. Usually CUT&RUN is Truseq and CUT&TAG is Nextera. If you don't know, use FastQC to determine if it is Truseq or Nextera - reference_genome: this field will be adapted to the genomes available for bowtie2 - effective_genome_size: this is used by macs2 and may be entered manually (indications are provided for heavily used genomes) +- normalize_profile: Whether you want to have a profile normalized as Signal to Million Reads. ## Processing - The workflow will remove illumina adapters and low quality bases and filter out any read smaller than 15bp - The filtered reads are mapped with bowtie2 allowing dovetail and fragment length up to 1kb - The BAM is filtered to keep only MAPQ30 and concordant pairs -- The PCR duplicates are removed with Picard +- The PCR duplicates are removed with Picard (only from version 0.6) - The BAM is converted to BED to enable macs2 to take both pairs into account -- The peaks are called with macs2 which at the same time generates a coverage file. +- The peaks are called with macs2 which at the same time generates a coverage file (normalized or not). - The coverage file is converted to bigwig - A multiQC is run to have an overview of the QC - -### Warning - -- The coverage output is not normalized. diff --git a/workflows/epigenetics/cutandrun/cutandrun-tests.yml b/workflows/epigenetics/cutandrun/cutandrun-tests.yml index 74692e828..8f2ebcecb 100644 --- a/workflows/epigenetics/cutandrun/cutandrun-tests.yml +++ b/workflows/epigenetics/cutandrun/cutandrun-tests.yml @@ -20,6 +20,7 @@ adapter_reverse: 'GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT' reference_genome: 'hg38canon' effective_genome_size: 2700000000 + normalize_profile: false outputs: Mapping stats: element_tests: @@ -34,8 +35,8 @@ Rep1: asserts: has_size: - value: 10914132 - delta: 1000000 + value: 8661584 + delta: 800000 MarkDuplicates metrics: element_tests: Rep1: @@ -59,7 +60,7 @@ has_text: text: "# tag size is determined as 40 bps" has_text_matching: - expression: "# total tags in treatment: 360634" + expression: "# total tags in treatment: 238930" - doc: Test outline for CUT&RUN (dm6) job: PE fastq input: @@ -82,6 +83,7 @@ adapter_reverse: 'GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT' reference_genome: 'dm6' effective_genome_size: 120000000 + normalize_profile: true outputs: Mapping stats: element_tests: @@ -121,7 +123,7 @@ has_text: text: "# tag size is determined as 36 bps" has_text_matching: - expression: "# total tags in treatment: 490338" + expression: "# total tags in treatment: 489716" Coverage from MACS2 (bigwig): element_tests: Rep1: diff --git a/workflows/epigenetics/cutandrun/cutandrun.ga b/workflows/epigenetics/cutandrun/cutandrun.ga index 34b356b4c..f288982e7 100644 --- a/workflows/epigenetics/cutandrun/cutandrun.ga +++ b/workflows/epigenetics/cutandrun/cutandrun.ga @@ -10,7 +10,7 @@ ], "format-version": "0.1", "license": "MIT", - "release": "0.1", + "release": "0.9", "name": "CUTandRUN", "steps": { "0": { @@ -37,6 +37,7 @@ "tool_version": null, "type": "data_collection_input", "uuid": "9fbb875d-05b1-4557-bd2e-710f80dd1a21", + "when": null, "workflow_outputs": [] }, "1": { @@ -63,6 +64,7 @@ "tool_version": null, "type": "parameter_input", "uuid": "1eac70a4-a4b7-42c7-82cd-913f23b4f941", + "when": null, "workflow_outputs": [] }, "2": { @@ -89,6 +91,7 @@ "tool_version": null, "type": "parameter_input", "uuid": "68b198e5-7de8-445f-807f-7f432090e2c7", + "when": null, "workflow_outputs": [] }, "3": { @@ -115,6 +118,7 @@ "tool_version": null, "type": "parameter_input", "uuid": "7f16a988-8ead-4a4a-9be1-8f5fbb744dec", + "when": null, "workflow_outputs": [] }, "4": { @@ -141,13 +145,41 @@ "tool_version": null, "type": "parameter_input", "uuid": "aae90433-355d-4f27-bdfb-77f8bc4fcff5", + "when": null, "workflow_outputs": [] }, "5": { - "annotation": "", - "content_id": "toolshed.g2.bx.psu.edu/repos/lparsons/cutadapt/cutadapt/4.0+galaxy0", + "annotation": "Whether you want to have a profile normalized as Signal to Million Reads", + "content_id": null, "errors": null, "id": 5, + "input_connections": {}, + "inputs": [ + { + "description": "Whether you want to have a profile normalized as Signal to Million Reads", + "name": "normalize_profile" + } + ], + "label": "normalize_profile", + "name": "Input parameter", + "outputs": [], + "position": { + "left": 228, + "top": 780.4016399999857 + }, + "tool_id": null, + "tool_state": "{\"parameter_type\": \"boolean\", \"optional\": false}", + "tool_version": null, + "type": "parameter_input", + "uuid": "a2c8d3e2-7542-4feb-8523-6100983ddb3b", + "when": null, + "workflow_outputs": [] + }, + "6": { + "annotation": "", + "content_id": "toolshed.g2.bx.psu.edu/repos/lparsons/cutadapt/cutadapt/4.7+galaxy0", + "errors": null, + "id": 6, "input_connections": { "library|input_1": { "id": 0, @@ -157,7 +189,7 @@ "id": 1, "output_name": "output" }, - "library|r2|adapters2_0|adapter_source2|adapter2": { + "library|r2|adapters2_0|adapter_source|adapter": { "id": 2, "output_name": "output" } @@ -203,27 +235,28 @@ "output_name": "report" } }, - "tool_id": "toolshed.g2.bx.psu.edu/repos/lparsons/cutadapt/cutadapt/4.0+galaxy0", + "tool_id": "toolshed.g2.bx.psu.edu/repos/lparsons/cutadapt/cutadapt/4.7+galaxy0", "tool_shed_repository": { - "changeset_revision": "02c5a84971c8", + "changeset_revision": "944ae523bacb", "name": "cutadapt", "owner": "lparsons", "tool_shed": "toolshed.g2.bx.psu.edu" }, - "tool_state": "{\"__job_resource\": {\"__job_resource__select\": \"no\", \"__current_case__\": 0}, \"adapter_options\": {\"action\": \"trim\", \"internal\": \"\", \"error_rate\": \"0.1\", \"no_indels\": \"false\", \"times\": \"1\", \"overlap\": \"3\", \"match_read_wildcards\": \" \", \"revcomp\": \"false\"}, \"filter_options\": {\"discard_trimmed\": \"false\", \"discard_untrimmed\": \"false\", \"minimum_length\": \"15\", \"maximum_length\": null, \"length_R2_options\": {\"length_R2_status\": \"False\", \"__current_case__\": 1}, \"max_n\": null, \"pair_filter\": \"any\", \"max_expected_errors\": null, \"discard_cassava\": \"false\"}, \"library\": {\"type\": \"paired_collection\", \"__current_case__\": 2, \"input_1\": {\"__class__\": \"RuntimeValue\"}, \"r1\": {\"adapters\": [{\"__index__\": 0, \"adapter_source\": {\"adapter_source_list\": \"user\", \"__current_case__\": 0, \"adapter_name\": \"Please use: For R1: - 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{ - "label": "MultiQC webpage", - "output_name": "html_report", - "uuid": "6d87436a-550e-4d76-a5da-d4463dfd4473" - }, { "label": "MultiQC on input dataset(s): Stats", "output_name": "stats", "uuid": "59dfba75-8658-4213-8fd9-b3ce5a916f14" + }, + { + "label": "MultiQC webpage", + "output_name": "html_report", + "uuid": "6d87436a-550e-4d76-a5da-d4463dfd4473" } ] } @@ -776,6 +843,6 @@ "tags": [ "CUTnRUN" ], - "uuid": "de155358-1b05-49ea-8740-0e22b5609a67", - "version": 6 + "uuid": "98e9e34b-b203-43db-9a23-5fa72963fa6d", + "version": 1 } \ No newline at end of file diff --git a/workflows/epigenetics/hic-hicup-cooler/.dockstore.yml b/workflows/epigenetics/hic-hicup-cooler/.dockstore.yml new file mode 100644 index 000000000..6d9404165 --- /dev/null +++ b/workflows/epigenetics/hic-hicup-cooler/.dockstore.yml @@ -0,0 +1,38 @@ +version: 1.2 +workflows: +- name: hic-fastq-to-cool-hicup-cooler + subclass: Galaxy + publish: true + primaryDescriptorPath: /hic-fastq-to-cool-hicup-cooler.ga + testParameterFiles: + - /hic-fastq-to-cool-hicup-cooler-tests.yml + authors: + - name: Lucille Delisle + orcid: 0000-0002-1964-4960 +- name: chic-fastq-to-cool-hicup-cooler + subclass: Galaxy + publish: true + primaryDescriptorPath: /chic-fastq-to-cool-hicup-cooler.ga + testParameterFiles: + - /chic-fastq-to-cool-hicup-cooler-tests.yml + authors: + - name: Lucille Delisle + orcid: 0000-0002-1964-4960 +- name: hic-juicermediumtabix-to-cool-cooler + subclass: Galaxy + publish: true + primaryDescriptorPath: /hic-juicermediumtabix-to-cool-cooler.ga + testParameterFiles: + - /hic-juicermediumtabix-to-cool-cooler-tests.yml + authors: + - name: Lucille Delisle + orcid: 0000-0002-1964-4960 +- name: hic-fastq-to-pairs-hicup + subclass: Galaxy + publish: true + primaryDescriptorPath: /hic-fastq-to-pairs-hicup.ga + testParameterFiles: + - /hic-fastq-to-pairs-hicup-tests.yml + authors: + - name: Lucille Delisle + orcid: 0000-0002-1964-4960 diff --git a/workflows/epigenetics/hic-hicup-cooler/.workflowhub.yml b/workflows/epigenetics/hic-hicup-cooler/.workflowhub.yml new file mode 100644 index 000000000..c30872e7a --- /dev/null +++ b/workflows/epigenetics/hic-hicup-cooler/.workflowhub.yml @@ -0,0 +1,5 @@ +version: '0.1' +registries: +- url: https://workflowhub.eu + project: iwc + workflow: hic-hicup-cooler/hic-fastq-to-cool-hicup-cooler diff --git a/workflows/epigenetics/hic-hicup-cooler/CHANGELOG.md b/workflows/epigenetics/hic-hicup-cooler/CHANGELOG.md new file mode 100644 index 000000000..86f34616a --- /dev/null +++ b/workflows/epigenetics/hic-hicup-cooler/CHANGELOG.md @@ -0,0 +1,26 @@ +# Changelog + +## [0.3] 2023-09-08 + +### Update tools +- all cooler tools were updated from 0.8.11 to 0.9.3 +- pyGenomeTracks was updated from 3.7 to 3.8 + +## [0.2.1] 2023-09-01 Bug fix + +Fix dockstore (workflows are the same) + +## [0.2] 2023-02-17 + +### Automatic update +- `toolshed.g2.bx.psu.edu/repos/iuc/pygenometracks/pygenomeTracks/3.7+galaxy0` was updated to `toolshed.g2.bx.psu.edu/repos/iuc/pygenometracks/pygenomeTracks/3.8+galaxy0` +- `toolshed.g2.bx.psu.edu/repos/bgruening/hicup_hicup/hicup_hicup/0.8.3+galaxy0` was updated to `toolshed.g2.bx.psu.edu/repos/bgruening/hicup_hicup/hicup_hicup/0.9.2+galaxy0` +- `toolshed.g2.bx.psu.edu/repos/bgruening/hicup2juicer/hicup2juicer/0.8.3+galaxy0` was updated to `toolshed.g2.bx.psu.edu/repos/bgruening/hicup2juicer/hicup2juicer/0.9.2+galaxy0` + +### Manual update +- Get fragment id in valid pairs file. +- Use the middle of the fragment instead of 5' of the read in the valid pairs file. + +## [0.1] 2023-01-16 + +First release. diff --git a/workflows/epigenetics/hic-hicup-cooler/README.md b/workflows/epigenetics/hic-hicup-cooler/README.md new file mode 100644 index 000000000..4c3d4f596 --- /dev/null +++ b/workflows/epigenetics/hic-hicup-cooler/README.md @@ -0,0 +1,62 @@ +# Hi-C (hic_fastq_to_cool_hicup_cooler) and region capture Hi-C (chic_fastq_to_cool_hicup_cooler) Workflows + +This can also be used for Hi-ChIP experiments, in that case the output with `matrix with iced values` is ignored and the matrix to use is `matrix with raw values`. + +## Input datasets + +- The workflow needs a list of dataset pairs of fastqsanger. + +## Input values + +- genome name: suggested from the bowtie2 indices, it is used to map and build the list of bins. +- restriction enzyme: Restriction enzyme used e.g. A^GATCT,BglII. The '^' is used to express where the enzyme cuts. +- No fill-in: If you used a biotin fill-in protocol, put this to false, else, put it to true. +- minimum MAPQ: Filtering to apply to pairs you want to keep in your matrix, set it to 0 to not apply filtering (HiCUP already filter for uniquely mapped or MAPQ30). +- Bin size in bp: Used to generate your first matrix but you will be able to rerun the subworkflow `hic_tabix_to_cool_cooler` to get other resolutions. +- Interactions to consider to calculate weights in normalization step: this is a parameter for the last correction step (ICE). + +For the region capture workflow: + +- chromosome, start and end positions of the capture region + +For the Hi-C workflow: + +- region to use in pyGenomeTracks to check the matrices. + +## Processing + +- Reads are processed with HiCUP which comprises these steps: + - Truncation of reads for the religation motif + - Mapping of mates independently with bowtie2 + - Pairing the mates when both mates are uniquely mapped or MAPQ30 + - Filtering the pairs for undigested, self-ligated... + - Removing duplicates +- The output BAM file is converted to medium juicer format: ` ` where str = strand (0 for forward, anything else for reverse) and pos is the middle of the fragment. +- The pairs are filtered for MAPQ if specified. +- For the region capture Hi-C workflow the pairs are filtered for both mates in the captured region. +- The filtered pairs are sorted and indexed with cooler_csort. +- The pairs are loaded into a matrix of the given resolution and balanced with cooler. +- A final plot is made with pyGenomeTracks using the balanced matrices on the region provided or the capture region. + +## Subworkflows + +There are 2 subworkflows: `hic_tabix_to_cool_cooler` and `hic_fastq_to_pairs_hicup.ga`. + +### hic_tabix_to_cool_cooler + +This first subworkflow can be used to generate matrices to different resolutions using one of the output of the full workflow (`valid pairs filtered and sorted`). + +If the dataset are still in galaxy (format: juicer_medium_tabix.gz), the workflow can be run directly. + +If the dataset is not anymore in galaxy, you need to upload and specify the datatype as: juicer_medium_tabix.gz + +### hic_fastq_to_pairs_hicup + +The second subworkflow has no real reason to be launched by itself except for QC tests. + +If you want to run the first subworkflow from these results: + +- You first need to filter the pairs (`valid pairs in juicebox format MAPQ filtered`) for the capture region if relevent using the tool Filter1 (**Filter** data on any column using simple expressions) with the condition `(c3=='chr2' and c4<180000000 and c4>170000000) and (c7=="chr2" and c8<180000000 and c8>170000000)` if your capture region is chr2:170000000-180000000. +- Then you need to run cooler_csort (**cooler csort with tabix** Sort and index a contact list.) with as input the `valid pairs in juicebox format MAPQ filtered` or the output of the previous step and for "Format of your input file" use "Juicer Medium Format". + +The output of `cooler_csort` can be used as input of the first subworkflow. diff --git a/workflows/epigenetics/hic-hicup-cooler/chic-fastq-to-cool-hicup-cooler-tests.yml b/workflows/epigenetics/hic-hicup-cooler/chic-fastq-to-cool-hicup-cooler-tests.yml new file mode 100644 index 000000000..173062097 --- /dev/null +++ b/workflows/epigenetics/hic-hicup-cooler/chic-fastq-to-cool-hicup-cooler-tests.yml @@ -0,0 +1,87 @@ +- doc: Test outline for Hi-C_fastqToCool_hicup_cooler + job: + PE fastq input: + class: Collection + collection_type: list:paired + elements: + - class: Collection + type: paired + identifier: test_dataset + elements: + - class: File + identifier: forward + location: https://github.com/bgruening/galaxytools/raw/master/tools/hicup/test-data/dataset1.fastq + filetype: fastqsanger + - class: File + identifier: reverse + location: https://github.com/bgruening/galaxytools/raw/master/tools/hicup/test-data/dataset2.fastq + filetype: fastqsanger + genome name: hg19 + Restriction enzyme: A^AGCTT,HindIII + No fill-in: 'false' + minimum MAPQ: 30 + Bin size in bp: 1000000 + Interactions to consider to calculate weights in normalization step: --cis-only + capture region (chromosome): chr2 + capture region (start): 170000000 + capture region (end): 180000000 + outputs: + HiCUP report (html): + element_tests: + test_dataset: + asserts: + has_size: + value: 4602173 + delta: 4000000 + HiCUP report (txt): + element_tests: + test_dataset: + asserts: + has_text: + text: "\t99742\t99742\t92512\t92628\t7230\t7114\t22.45\t22.63\t2658\t2476\t73431\t72142\t17767\t18475\t5886\t6649\t57671\t57671\t39966\t1652\t17997\t20317\t17705\t481\t2432\t13452\t1340\t0\t0\t39962\t1652\t17996\t20314\t57.82\t69.30\t99.99\t50.83\t40.07" + valid pairs in juicebox format: + element_tests: + test_dataset: + asserts: + - that: "has_line" + line: "1 1 chr10 100023987 28055 1 chr10 101500419 28474 42 42" + - that: "has_line" + line: "2 1 chr10 100091500 28079 1 chr10 122245984 34516 38 42" + - that: "has_line" + line: "3 0 chr10 100127492 28094 1 chr10 50864290 13489 0 42" + valid pairs in juicebox format MAPQ filtered: + element_tests: + test_dataset: + asserts: + - that: "has_line" + line: "1 1 chr10 100023987 28055 1 chr10 101500419 28474 42 42" + - that: "has_line" + line: "2 1 chr10 100091500 28079 1 chr10 122245984 34516 38 42" + - that: "not_has_text" + text: "3 0 chr10 100127492 28094 1 chr10 50864290 13489 0 42" + valid pairs filtered and sorted: + element_tests: + test_dataset: + asserts: + has_size: + value: 807 + delta: 80 + matrix with raw values: + element_tests: + test_dataset: + asserts: + has_size: + value: 42118 + delta: 4000 + matrix with iced values: + element_tests: + test_dataset: + asserts: + has_size: + value: 47830 + delta: 4000 + plot with pyGenomeTracks: + class: File + file: test-data/plot_chic.png + compare: sim_size + delta: 1500 diff --git a/workflows/epigenetics/hic-hicup-cooler/chic-fastq-to-cool-hicup-cooler.ga b/workflows/epigenetics/hic-hicup-cooler/chic-fastq-to-cool-hicup-cooler.ga new file mode 100644 index 000000000..903a8e783 --- /dev/null +++ b/workflows/epigenetics/hic-hicup-cooler/chic-fastq-to-cool-hicup-cooler.ga @@ -0,0 +1,1427 @@ +{ + "a_galaxy_workflow": "true", + "annotation": "This workflow take as input a collection of paired fastq. It uses HiCUP to go from fastq to validPair file. The pairs are filtered for MAPQ and for the region captured. Then, they are sorted by cooler to generate a tabix dataset. Cooler is used to generate a balanced cool file to the desired resolution.", + "creator": [ + { + "class": "Person", + "identifier": "https://orcid.org/0000-0002-1964-4960", + "name": "Lucille Delisle" + } + ], + "format-version": "0.1", + "license": "MIT", + "release": "0.3", + "name": "cHi-C_fastqToCool_hicup_cooler", + "steps": { + "0": { + "annotation": "Should be a paired collection with Hi-C fastqs", + "content_id": null, + "errors": null, + "id": 0, + "input_connections": {}, + "inputs": [ + { + "description": "Should be a paired collection with Hi-C fastqs", + "name": "PE fastq input" + } + ], + "label": "PE fastq input", + "name": "Input dataset collection", + "outputs": [], + "position": { + "left": 0, + "top": 39.12167999999406 + }, + "tool_id": null, + "tool_state": "{\"optional\": false, \"tag\": null, \"collection_type\": \"list:paired\"}", + "tool_version": null, + "type": "data_collection_input", + "uuid": "30fe3d0f-541a-478a-b57d-a43c0c16ccad", + "when": null, + "workflow_outputs": [] + }, + "1": { + "annotation": "only use genome ids which have bowtie2 indexes", + "content_id": null, + "errors": null, + "id": 1, + "input_connections": {}, + "inputs": [ + { + "description": "only use genome ids which have bowtie2 indexes", + "name": "genome name" + } + ], + "label": "genome name", + "name": "Input parameter", + "outputs": [], + "position": { + "left": 39, + "top": 109.12167999999406 + }, + "tool_id": null, + "tool_state": "{\"restrictOnConnections\": true, \"parameter_type\": \"text\", \"optional\": false}", + "tool_version": null, + "type": "parameter_input", + "uuid": "45194ed6-a1e9-4248-8d3a-f51febc36d61", + "when": null, + "workflow_outputs": [] + }, + "2": { + "annotation": "Restriction enzyme used e.g. A^GATCT,BglII. 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HiCUP accomodates N in restriction enzyme: e.g. :A^ANCTT", + "name": "Restriction enzyme" + } + ], + "label": "Restriction enzyme", + "name": "Input parameter", + "outputs": [], + "position": { + "left": 72, + "top": 184.12167999999406 + }, + "tool_id": null, + "tool_state": "{\"parameter_type\": \"text\", \"optional\": false}", + "tool_version": null, + "type": "parameter_input", + "uuid": "ec011ceb-c601-464f-8661-ca9c0b335bb5", + "when": null, + "workflow_outputs": [] + }, + "3": { + "annotation": "Hi-C protocol did NOT include a fill-in of sticky ends prior to re-ligation and therefore reads shall be truncated at the restriction site sequence", + "content_id": null, + "errors": null, + "id": 3, + "input_connections": {}, + "inputs": [ + { + "description": "Hi-C protocol did NOT include a fill-in of sticky ends prior to re-ligation and therefore reads shall be truncated at the restriction site sequence", + "name": "No fill-in" + } + ], + "label": "No fill-in", + "name": "Input 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mode 100644 index 000000000..95a438d6b --- /dev/null +++ b/workflows/epigenetics/hic-hicup-cooler/hic-fastq-to-cool-hicup-cooler-tests.yml @@ -0,0 +1,85 @@ +- doc: Test outline for Hi-C_fastqToCool_hicup_cooler + job: + PE fastq input: + class: Collection + collection_type: list:paired + elements: + - class: Collection + type: paired + identifier: test_dataset + elements: + - class: File + identifier: forward + location: https://github.com/bgruening/galaxytools/raw/master/tools/hicup/test-data/dataset1.fastq + filetype: fastqsanger + - class: File + identifier: reverse + location: https://github.com/bgruening/galaxytools/raw/master/tools/hicup/test-data/dataset2.fastq + filetype: fastqsanger + genome name: hg19 + Restriction enzyme: A^AGCTT,HindIII + No fill-in: 'false' + minimum MAPQ: 30 + Bin size in bp: 1000000 + Interactions to consider to calculate weights in normalization step: "" + region for matrix plotting: chr2:175,689,620-178,604,461 + outputs: + HiCUP report (html): + element_tests: + test_dataset: + asserts: + has_size: + value: 4602173 + delta: 4000000 + HiCUP report (txt): + element_tests: + test_dataset: + asserts: + has_text: + text: "\t99742\t99742\t92512\t92628\t7230\t7114\t22.45\t22.63\t2658\t2476\t73431\t72142\t17767\t18475\t5886\t6649\t57671\t57671\t39966\t1652\t17997\t20317\t17705\t481\t2432\t13452\t1340\t0\t0\t39962\t1652\t17996\t20314\t57.82\t69.30\t99.99\t50.83\t40.07" + valid pairs in juicebox format: + element_tests: + test_dataset: + asserts: + - that: "has_line" + line: "1 1 chr10 100023987 28055 1 chr10 101500419 28474 42 42" + - that: "has_line" + line: "2 1 chr10 100091500 28079 1 chr10 122245984 34516 38 42" + - that: "has_line" + line: "3 0 chr10 100127492 28094 1 chr10 50864290 13489 0 42" + valid pairs in juicebox format MAPQ filtered: + element_tests: + test_dataset: + asserts: + - that: "has_line" + line: "1 1 chr10 100023987 28055 1 chr10 101500419 28474 42 42" + - that: "has_line" + line: "2 1 chr10 100091500 28079 1 chr10 122245984 34516 38 42" + - that: "not_has_text" + text: "3 0 chr10 100127492 28094 1 chr10 50864290 13489 0 42" + valid pairs filtered and sorted: + element_tests: + test_dataset: + asserts: + has_size: + value: 735227 + delta: 70000 + matrix with raw values: + element_tests: + test_dataset: + asserts: + has_size: + value: 117588 + delta: 10000 + matrix with iced values: + element_tests: + test_dataset: + asserts: + has_size: + value: 149349 + delta: 10000 + plot with pyGenomeTracks: + class: File + file: test-data/plot_hic.png + compare: sim_size + delta: 1500 diff --git a/workflows/epigenetics/hic-hicup-cooler/hic-fastq-to-cool-hicup-cooler.ga b/workflows/epigenetics/hic-hicup-cooler/hic-fastq-to-cool-hicup-cooler.ga new file mode 100644 index 000000000..71ea93dc9 --- /dev/null +++ b/workflows/epigenetics/hic-hicup-cooler/hic-fastq-to-cool-hicup-cooler.ga @@ -0,0 +1,1212 @@ +{ + "a_galaxy_workflow": "true", + "annotation": "This workflow takes as input a collection of paired 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https://github.com/bgruening/galaxytools/raw/master/tools/hicup/test-data/dataset2.fastq + filetype: fastqsanger + genome name: hg19 + Restriction enzyme: A^AGCTT,HindIII + No fill-in: 'false' + minimum MAPQ: 30 + outputs: + HiCUP report (HTML): + element_tests: + test_dataset: + asserts: + has_size: + value: 4602173 + delta: 4000000 + HiCUP report (tabular): + element_tests: + test_dataset: + asserts: + has_text: + text: "\t99742\t99742\t92512\t92628\t7230\t7114\t22.45\t22.63\t2658\t2476\t73431\t72142\t17767\t18475\t5886\t6649\t57671\t57671\t39966\t1652\t17997\t20317\t17705\t481\t2432\t13452\t1340\t0\t0\t39962\t1652\t17996\t20314\t57.82\t69.30\t99.99\t50.83\t40.07" + valid pairs in juicebox format: + element_tests: + test_dataset: + asserts: + - that: "has_line" + line: "1 1 chr10 100023987 28055 1 chr10 101500419 28474 42 42" + - that: "has_line" + line: "2 1 chr10 100091500 28079 1 chr10 122245984 34516 38 42" + - that: "has_line" + line: "3 0 chr10 100127492 28094 1 chr10 50864290 13489 0 42" + valid pairs in juicebox format MAPQ filtered: + element_tests: + test_dataset: + asserts: + - that: "has_line" + line: "1 1 chr10 100023987 28055 1 chr10 101500419 28474 42 42" + - that: "has_line" + line: "2 1 chr10 100091500 28079 1 chr10 122245984 34516 38 42" + - that: "not_has_text" + text: "3 0 chr10 100127492 28094 1 chr10 50864290 13489 0 42" diff --git a/workflows/epigenetics/hic-hicup-cooler/hic-fastq-to-pairs-hicup.ga b/workflows/epigenetics/hic-hicup-cooler/hic-fastq-to-pairs-hicup.ga new file mode 100644 index 000000000..28c9666b7 --- /dev/null +++ b/workflows/epigenetics/hic-hicup-cooler/hic-fastq-to-pairs-hicup.ga @@ -0,0 +1,435 @@ +{ + "a_galaxy_workflow": "true", + "annotation": "This workflow takes as input a collection of paired fastq. 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"uuid": "48f579ea-eaac-49ee-b38f-93c82f8b7d76", + "version": 2 +} diff --git a/workflows/epigenetics/hic-hicup-cooler/hic-juicermediumtabix-to-cool-cooler-tests.yml b/workflows/epigenetics/hic-hicup-cooler/hic-juicermediumtabix-to-cool-cooler-tests.yml new file mode 100644 index 000000000..a81211d06 --- /dev/null +++ b/workflows/epigenetics/hic-hicup-cooler/hic-juicermediumtabix-to-cool-cooler-tests.yml @@ -0,0 +1,28 @@ +- doc: Test outline for Hi-C_fastqToCool_hicup_cooler + job: + Juicer Medium Tabix with validPairs: + class: Collection + collection_type: list + elements: + - class: File + identifier: test_dataset + path: test-data/valid_pairs_filtered_and_sorted.juicer_medium_tabix.gz + filetype: juicer_medium_tabix.gz + genome name: hg19 + Bin size in bp: 1000000 + Interactions to consider to calculate weights in normalization step: --cis-only + outputs: + matrix with raw values: + element_tests: + test_dataset: + asserts: + has_size: + value: 42118 + delta: 4000 + matrix with iced values: + element_tests: + test_dataset: + asserts: + has_size: + value: 47830 + delta: 4000 diff --git a/workflows/epigenetics/hic-hicup-cooler/hic-juicermediumtabix-to-cool-cooler.ga b/workflows/epigenetics/hic-hicup-cooler/hic-juicermediumtabix-to-cool-cooler.ga new file mode 100644 index 000000000..408e5ca35 --- /dev/null +++ b/workflows/epigenetics/hic-hicup-cooler/hic-juicermediumtabix-to-cool-cooler.ga @@ -0,0 +1,302 @@ +{ + "a_galaxy_workflow": "true", + "annotation": "This workflow uses as input a collection of juicer medium tabix files and a genome name. It builds balanced cool file to the desired resolution.", + "creator": [ + { + "class": "Person", + "identifier": "https://orcid.org/0000-0002-1964-4960", + "name": "Lucille Delisle" + } + ], + "format-version": "0.1", + "license": "MIT", + "release": "0.3", + "name": "Hi-C_juicermediumtabixToCool_cooler", + "steps": { + "0": { + "annotation": "For example 10000 for 10kb", + "content_id": null, + "errors": null, + "id": 0, + "input_connections": {}, + "inputs": [ + { + "description": "For example 10000 for 10kb", + "name": "Bin size in bp" + } + ], + "label": "Bin size in bp", + "name": "Input parameter", + "outputs": [], + "position": { + "left": 0, + "top": 33.5 + }, + "tool_id": null, + "tool_state": "{\"parameter_type\": \"integer\", \"optional\": false}", + "tool_version": null, + "type": "parameter_input", + "uuid": "d0a82863-82f8-426c-addb-06066e9d0a73", + "when": null, + "workflow_outputs": [] + }, + "1": { + "annotation": "This is used to get the chromosome sizes", + 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000000000..92a6bbb26 Binary files /dev/null and b/workflows/epigenetics/hic-hicup-cooler/test-data/plot_chic.png differ diff --git a/workflows/epigenetics/hic-hicup-cooler/test-data/plot_hic.png b/workflows/epigenetics/hic-hicup-cooler/test-data/plot_hic.png new file mode 100644 index 000000000..2d856170e Binary files /dev/null and b/workflows/epigenetics/hic-hicup-cooler/test-data/plot_hic.png differ diff --git a/workflows/epigenetics/hic-hicup-cooler/test-data/valid_pairs_filtered_and_sorted.juicer_medium_tabix.gz b/workflows/epigenetics/hic-hicup-cooler/test-data/valid_pairs_filtered_and_sorted.juicer_medium_tabix.gz new file mode 100644 index 000000000..bcbcd4fd5 Binary files /dev/null and b/workflows/epigenetics/hic-hicup-cooler/test-data/valid_pairs_filtered_and_sorted.juicer_medium_tabix.gz differ diff --git a/workflows/gen_crates.py b/workflows/gen_crates.py index 8fd492ba4..9b8e08ac8 100644 --- a/workflows/gen_crates.py +++ b/workflows/gen_crates.py @@ -60,8 +60,6 @@ TARGET_OWNER = "iwc-workflows" GH_API_URL = "https://api.github.com" PLANEMO_VERSION = f">={planemo.__version__}" -PLANEMO_TEST_SUFFIXES = ["-tests", "_tests", "-test", "_test"] -PLANEMO_TEST_EXTENSIONS = [".yml", ".yaml", ".json"] HUB_URL = "https://workflowhub.eu" HUB_API_KEY = os.getenv("HUB_API_KEY") HUB_CFG_NAME = ".workflowhub.yml" @@ -175,24 +173,6 @@ def update_wf_access(self, wf_id, proj_id): return self.patch(f"/workflows/{wf_id}", payload=payload) -def get_wf_id(crate_dir): - ids = [_.name for _ in os.scandir(crate_dir) if _.name.endswith(".ga")] - if not ids: - raise RuntimeError(".ga workflow file not found") - return ids[0] - - -def get_planemo_id(crate_dir, wf_id): - tag, _ = os.path.splitext(wf_id) - for suffix in PLANEMO_TEST_SUFFIXES: - for ext in PLANEMO_TEST_EXTENSIONS: - planemo_id = f"{tag}{suffix}{ext}" - planemo_source = Path(crate_dir) / planemo_id - if planemo_source.is_file(): - return planemo_id, planemo_source - raise RuntimeError(f"Planemo test file not found in {crate_dir}") - - def handle_creator(ga_json, crate, workflow): try: gh_creators = ga_json["creator"] @@ -218,29 +198,29 @@ def handle_creator(ga_json, crate, workflow): workflow["creator"] = ro_creators -def get_workflow_name(repo_dir): - repo_dir = Path(repo_dir) +def get_wf_records(repo_dir): cfg_path = repo_dir / DOCKSTORE_CFG_NAME if not cfg_path.is_file(): raise RuntimeError(f"{cfg_path} not found") with open(cfg_path, "rt") as f: cfg = yaml.load(f, Loader=Loader) assert str(cfg["version"]) == DOCKSTORE_CFG_VERSION - wf_entry = cfg["workflows"][0] # assuming first wf is the main wf - return f"{repo_dir.name}/{wf_entry['name']}" + return cfg["workflows"] -def make_crate(crate_dir, target_owner, planemo_version): - wf_id = get_wf_id(crate_dir) - planemo_id, planemo_source = get_planemo_id(crate_dir, wf_id) - crate = ROCrate(gen_preview=False) - wf_source = Path(crate_dir) / wf_id +def add_workflow(crate_dir, crate, wf_record, target_owner, planemo_version, main=False): + wf_id = wf_record["primaryDescriptorPath"].lstrip("/") + wf_source = crate_dir / wf_id + if not wf_source.is_file(): + return with open(wf_source) as f: code = json.load(f) - workflow = crate.add_workflow(wf_source, wf_id, main=True, + workflow = crate.add_workflow(wf_source, wf_id, main=main, lang="galaxy", gen_cwl=False) handle_creator(code, crate, workflow) - workflow["name"] = crate.root_dataset["name"] = get_workflow_name(crate_dir) + workflow["name"] = wf_name = f"{crate_dir.name}/{wf_record['name']}" + if main: + crate.root_dataset["name"] = wf_name try: workflow["version"] = code["release"] except KeyError: @@ -251,16 +231,31 @@ def make_crate(crate_dir, target_owner, planemo_version): crate.root_dataset["license"] = code["license"] except KeyError: pass - readme_source = Path(crate_dir) / "README.md" + readme_source = crate_dir / "README.md" if readme_source.is_file(): crate.add_file(readme_source, "README.md") - suite = crate.add_test_suite(identifier="#test1") - resource = f"repos/{target_owner}/{crate_dir.name}/actions/workflows/{CI_WORKFLOW.name}" - crate.add_test_instance(suite, GH_API_URL, resource=resource, - service="github", identifier="test1_1") - crate.add_test_definition(suite, source=planemo_source, - dest_path=planemo_id, engine="planemo", - engine_version=planemo_version) + for i, planemo_id in enumerate(wf_record.get("testParameterFiles", [])): + planemo_id = planemo_id.lstrip("/") + planemo_source = Path(crate_dir) / planemo_id + if not planemo_source.is_file(): + continue + suite_id = f"#test_{wf_record['name']}_{i+1}" + instance_id = f"{suite_id}_1" + suite = crate.add_test_suite(identifier=suite_id, main_entity=workflow) + resource = f"repos/{target_owner}/{crate_dir.name}/actions/workflows/{CI_WORKFLOW.name}" + crate.add_test_instance(suite, GH_API_URL, resource=resource, + service="github", identifier=instance_id) + crate.add_test_definition(suite, source=planemo_source, + dest_path=planemo_id, engine="planemo", + engine_version=planemo_version) + + +def make_crate(crate_dir, target_owner, planemo_version): + crate = ROCrate(gen_preview=False) + wf_records = get_wf_records(crate_dir) + for i, r in enumerate(wf_records): + main = i == 0 + add_workflow(crate_dir, crate, r, target_owner, planemo_version, main=main) crate.metadata.write(crate_dir) diff --git a/workflows/genome-assembly/assembly-with-flye/.dockstore.yml b/workflows/genome-assembly/assembly-with-flye/.dockstore.yml new file mode 100644 index 000000000..61147c1ea --- /dev/null +++ b/workflows/genome-assembly/assembly-with-flye/.dockstore.yml @@ -0,0 +1,11 @@ +version: 1.2 +workflows: +- name: main + subclass: Galaxy + publish: true + primaryDescriptorPath: /Genome-assembly-with-Flye.ga + testParameterFiles: + - /Genome-assembly-with-Flye-tests.yml + authors: + - name: Anna Syme + orcid: 0000-0002-9906-0673 diff --git a/workflows/genome-assembly/assembly-with-flye/CHANGELOG.md b/workflows/genome-assembly/assembly-with-flye/CHANGELOG.md new file mode 100644 index 000000000..00e1f7d95 --- /dev/null +++ b/workflows/genome-assembly/assembly-with-flye/CHANGELOG.md @@ -0,0 +1,14 @@ +# Changelog + +## [0.2] 2024-03-25 + +### Automatic update +- `toolshed.g2.bx.psu.edu/repos/bgruening/flye/flye/2.9.1+galaxy0` was updated to `toolshed.g2.bx.psu.edu/repos/bgruening/flye/flye/2.9.3+galaxy0` + +All notable changes to this project will be documented in this file. + +The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/), +and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). + +## [0.1] 2023-07-14 +First release. diff --git a/workflows/genome-assembly/assembly-with-flye/Genome-assembly-with-Flye-tests.yml b/workflows/genome-assembly/assembly-with-flye/Genome-assembly-with-Flye-tests.yml new file mode 100644 index 000000000..2e59d3fb4 --- /dev/null +++ b/workflows/genome-assembly/assembly-with-flye/Genome-assembly-with-Flye-tests.yml @@ -0,0 +1,46 @@ +- doc: Test outline for Genome-assembly-with-Flye + job: + Input sequence reads: + class: File + path: test-data/Input_reads.fastqsanger.gz + filetype: fastqsanger.gz + outputs: + Flye assembly (consensus): + asserts: + has_text: + text: '>contig_1' + has_size: + min: 80k + max: 86k + Flye assembly (assembly_graph): + asserts: + has_text: + text: 'digraph {' + has_n_lines: + min: 20 + max: 24 + Flye assembly (Graphical Fragment Assembly): + asserts: + has_text: + text: edge_1 + has_n_lines: + min: 6 + max: 8 + Flye assembly (assembly_info): + asserts: + has_text: + text: seq_name + 'Quast: HTML report': + asserts: + has_size: + min: 250k + max: 400k + Flye assembly statistics: + asserts: + has_text: + text: "Scaffold L50" + 'Bandage Image: Assembly Graph Image': + asserts: + has_size: + min: 30k + max: 50k diff --git a/workflows/genome-assembly/assembly-with-flye/Genome-assembly-with-Flye.ga b/workflows/genome-assembly/assembly-with-flye/Genome-assembly-with-Flye.ga new file mode 100644 index 000000000..2de7b57cd --- /dev/null +++ b/workflows/genome-assembly/assembly-with-flye/Genome-assembly-with-Flye.ga @@ -0,0 +1,265 @@ +{ + "a_galaxy_workflow": "true", + "annotation": "Assemble long reads with Flye, then view assembly statistics and assembly graph", + "creator": [ + { + "class": "Person", + "identifier": "https://orcid.org/0000-0002-9906-0673", + "name": "Anna Syme" + } + ], + "format-version": "0.1", + "license": "MIT", + "release": "0.2", + "name": "Genome assembly with Flye", + "report": { + "markdown": "\n# Workflow Execution Report\n\n## Workflow Inputs\n```galaxy\ninvocation_inputs()\n```\n\n## Workflow Outputs\n```galaxy\ninvocation_outputs()\n```\n\n## Workflow\n```galaxy\nworkflow_display()\n```\n\n" + }, + "steps": { + "0": { + "annotation": "Sequence reads e.g. nanopore", + "content_id": null, + "errors": null, + "id": 0, + "input_connections": {}, + "inputs": [ + { + "description": "Sequence reads e.g. nanopore", + "name": "Input sequence reads" + } + ], + "label": "Input sequence reads", + "name": "Input dataset", + "outputs": [], + "position": { + "left": 0, + "top": 263.5110244255367 + }, + "tool_id": null, + "tool_state": "{\"optional\": false, \"tag\": null}", + "tool_version": null, + "type": "data_input", + "uuid": "501d7387-3bed-4510-891f-a4571a48d9ab", + "when": null, + "workflow_outputs": [] + }, + "1": { + "annotation": "", + "content_id": "toolshed.g2.bx.psu.edu/repos/bgruening/flye/flye/2.9.3+galaxy0", + "errors": null, + "id": 1, + "input_connections": { + "inputs": { + "id": 0, + "output_name": "output" + } + }, + "inputs": [], + "label": "Flye: assembly", + "name": "Flye", + "outputs": [ + { + "name": "consensus", + "type": "fasta" + }, + { + "name": "assembly_graph", + "type": "graph_dot" + }, + { + "name": "assembly_gfa", + "type": "txt" + }, + { + "name": "assembly_info", + "type": "tabular" + } + ], + "position": { + "left": 264.3488280182951, + "top": 139.5081025362239 + }, + "post_job_actions": { + "RenameDatasetActionassembly_gfa": { + "action_arguments": { + "newname": "Flye output as GFA for bandage" + }, + "action_type": "RenameDatasetAction", + "output_name": "assembly_gfa" + } + }, + "tool_id": "toolshed.g2.bx.psu.edu/repos/bgruening/flye/flye/2.9.3+galaxy0", + "tool_shed_repository": { + "changeset_revision": "291923e6f276", + "name": "flye", + "owner": "bgruening", + "tool_shed": "toolshed.g2.bx.psu.edu" + }, + "tool_state": "{\"asm\": {\"asm_select\": \"false\", \"__current_case__\": 1}, \"generate_log\": false, \"i\": \"1\", \"inputs\": {\"__class__\": \"ConnectedValue\"}, \"iterations\": \"1\", \"keep_haplotypes\": false, \"m\": null, \"meta\": false, \"min_overlap\": null, \"mode\": \"--nano-raw\", \"mode_conditional\": {\"mode\": \"--nano-raw\", \"__current_case__\": 0}, \"no_alt_contigs\": false, \"no_trestle\": \"false\", \"plasmids\": \"false\", \"scaffold\": false, \"__page__\": null, \"__rerun_remap_job_id__\": null}", + "tool_version": "2.9.3+galaxy0", + 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"report_html", + "type": "html" + } + ], + "position": { + "left": 562.9918212890625, + "top": 0 + }, + "post_job_actions": {}, + "tool_id": "toolshed.g2.bx.psu.edu/repos/iuc/quast/quast/5.2.0+galaxy1", + "tool_shed_repository": { + "changeset_revision": "72472698a2df", + "name": "quast", + "owner": "iuc", + "tool_shed": "toolshed.g2.bx.psu.edu" + }, + "tool_state": "{\"__job_resource\": {\"__current_case__\": 0, \"__job_resource__select\": \"no\"}, \"advanced\": {\"contig_thresholds\": \"0,1000\", \"strict_NA\": false, \"extensive_mis_size\": \"1000\", \"scaffold_gap_max_size\": \"1000\", \"unaligned_part_size\": \"500\", \"skip_unaligned_mis_contigs\": true, \"fragmented_max_indent\": null, \"report_all_metrics\": false, \"x_for_Nx\": \"90\"}, \"al\": {\"ambiguity_score\": \"0.99\", \"ambiguity_usage\": \"one\", \"fragmented\": false, \"fragmented_max_indent\": \"50\", \"min_alignment\": \"65\", \"min_identity\": \"95.0\", \"upper_bound_assembly\": false, \"upper_bound_min_con\": 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+ { + "name": "stats_output", + "type": "tabular" + } + ], + "position": { + "left": 569.7271118164062, + "top": 189.37097199902308 + }, + "post_job_actions": {}, + "tool_id": "toolshed.g2.bx.psu.edu/repos/iuc/fasta_stats/fasta-stats/2.0", + "tool_shed_repository": { + "changeset_revision": "0dbb995c7d35", + "name": "fasta_stats", + "owner": "iuc", + "tool_shed": "toolshed.g2.bx.psu.edu" + }, + "tool_state": "{\"dataset\": {\"__class__\": \"ConnectedValue\"}, \"fasta\": {\"__class__\": \"ConnectedValue\"}, \"gaps_option\": false, \"genome_size\": null, \"__page__\": null, \"__rerun_remap_job_id__\": null}", + "tool_version": "2.0", + "type": "tool", + "uuid": "811b5756-e24e-4de3-a3d6-2ee4bf347c1b", + "when": null, + "workflow_outputs": [ + { + "label": "Flye assembly statistics", + "output_name": "stats_output", + "uuid": "49f24151-e76c-4d94-8045-4d52bcd9aae5" + } + ] + }, + "4": { + "annotation": "", + "content_id": "toolshed.g2.bx.psu.edu/repos/iuc/bandage/bandage_image/2022.09+galaxy4", + "errors": null, + "id": 4, + "input_connections": { + "input_file": { + "id": 1, + "output_name": "assembly_gfa" + } + }, + "inputs": [], + "label": "Bandage image: Flye assembly", + "name": "Bandage Image", + "outputs": [ + { + "name": "outfile", + "type": "jpg" + } + ], + "position": { + "left": 571.0624389648438, + "top": 311.80291748046875 + }, + "post_job_actions": {}, + "tool_id": "toolshed.g2.bx.psu.edu/repos/iuc/bandage/bandage_image/2022.09+galaxy4", + "tool_shed_repository": { + "changeset_revision": "ddddce450736", + "name": "bandage", + "owner": "iuc", + "tool_shed": "toolshed.g2.bx.psu.edu" + }, + "tool_state": "{\"fontsize\": null, \"height\": \"1000\", \"input_file\": {\"__class__\": \"ConnectedValue\"}, \"lengths\": false, \"names\": false, \"nodewidth\": null, \"output_format\": \"jpg\", \"width\": null, \"__page__\": null, \"__rerun_remap_job_id__\": null}", + "tool_version": "2022.09+galaxy4", + "type": "tool", + "uuid": "395f2dfe-3fa5-4684-bf97-7dd12e970f78", + "when": null, + "workflow_outputs": [ + { + "label": "Bandage Image: Assembly Graph Image", + "output_name": "outfile", + "uuid": "e66bd129-146f-48dc-95b8-39a2a1ffb68d" + } + ] + } + }, + "tags": [], + "uuid": "83ef6fdc-b8a9-41af-a824-0225f0199e63", + "version": 21 +} \ No newline at end of file diff --git a/workflows/genome-assembly/assembly-with-flye/README.md b/workflows/genome-assembly/assembly-with-flye/README.md new file mode 100644 index 000000000..cf9e8f021 --- /dev/null +++ b/workflows/genome-assembly/assembly-with-flye/README.md @@ -0,0 +1,35 @@ +# Genome assembly with Flye workflow + + +## Why use this workflow? + +- This is a fairly simple workflow that assembles a genome from long sequencing reads. +- It takes in sequencing reads from PacBio (Hifi or non-Hifi), or Oxford Nanopore. +- If you have PacBio Hifi reads, you may prefer to use a workflow with the assembly tool Hifiasm, such as the those in the suite of VGP workflows. + +## Inputs + +Raw sequencing reads from PacBio or Oxford Nanopore in format: +fasta, fasta.gz, fastq, fastq.gz, fastqsanger.gz or fastqsanger + +## What does the workflow do + +- Assembles the reads with the tool Flye +- Summarizes the statistics with the tool Fasta statistics +- Report with the tool Quast +- Renders the assembly graph with the tool Bandage + +## Settings + +Run as-is or change parameters at runtime + +For example: +- change the Flye option of "mode" to the correct sequencing type +- change the Quast option for "Type of organism" to correct taxon + +## Outputs + +- Flye assembly output - four files: fasta, gfa for bandage, graph_dot file, assembly info +- Fasta statistics +- Bandage image +- Quast report diff --git a/workflows/genome-assembly/assembly-with-flye/test-data/Input_reads.fastqsanger.gz b/workflows/genome-assembly/assembly-with-flye/test-data/Input_reads.fastqsanger.gz new file mode 100644 index 000000000..2a61b4c51 Binary files /dev/null and b/workflows/genome-assembly/assembly-with-flye/test-data/Input_reads.fastqsanger.gz differ diff --git a/workflows/genome-assembly/polish-with-long-reads/.dockstore.yml b/workflows/genome-assembly/polish-with-long-reads/.dockstore.yml new file mode 100644 index 000000000..99237d302 --- /dev/null +++ b/workflows/genome-assembly/polish-with-long-reads/.dockstore.yml @@ -0,0 +1,11 @@ +version: 1.2 +workflows: +- name: main + subclass: Galaxy + publish: true + primaryDescriptorPath: /Assembly-polishing-with-long-reads.ga + testParameterFiles: + - /Assembly-polishing-with-long-reads-tests.yml + authors: + - name: Anna Syme + orcid: 0000-0002-9906-0673 diff --git a/workflows/genome-assembly/polish-with-long-reads/Assembly-polishing-with-long-reads-tests.yml b/workflows/genome-assembly/polish-with-long-reads/Assembly-polishing-with-long-reads-tests.yml new file mode 100644 index 000000000..134bbd87b --- /dev/null +++ b/workflows/genome-assembly/polish-with-long-reads/Assembly-polishing-with-long-reads-tests.yml @@ -0,0 +1,16 @@ +- doc: Test outline for Assembly-polishing-with-long-reads + job: + Assembly to be polished: + class: File + path: test-data/assembly.fasta + filetype: fasta + long reads: + class: File + path: test-data/long_reads.fastqsanger.gz + filetype: fastqsanger.gz + 'minimap setting (for long reads) ': map-ont + outputs: + Assembly polished by long reads using Racon: + path: test-data/assembly_polished_by_long_reads.fasta + compare: sim_size + delta_frac: 0.2 diff --git a/workflows/genome-assembly/polish-with-long-reads/Assembly-polishing-with-long-reads.ga b/workflows/genome-assembly/polish-with-long-reads/Assembly-polishing-with-long-reads.ga new file mode 100644 index 000000000..a2d19082d --- /dev/null +++ b/workflows/genome-assembly/polish-with-long-reads/Assembly-polishing-with-long-reads.ga @@ -0,0 +1,533 @@ +{ + "a_galaxy_workflow": "true", + "annotation": "Racon polish with 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\"include_unpolished\": false, \"m\": \"5\", \"match\": \"3\", \"mismatch\": \"-5\", \"no_trimming\": false, \"overlaps\": {\"__class__\": \"ConnectedValue\"}, \"q\": \"10.0\", \"quality_threshold\": \"10.0\", \"reads\": {\"__class__\": \"ConnectedValue\"}, \"u\": \"true\", \"w\": \"500\", \"window_length\": \"500\", \"x\": \"-4\", \"__page__\": null, \"__rerun_remap_job_id__\": null}", + "tool_version": "1.5.0+galaxy1", + "type": "tool", + "uuid": "53f4572e-3a4a-4585-89ab-9459f8a61720", + "when": null, + "workflow_outputs": [ + { + "label": "Assembly polished by long reads using Racon", + "output_name": "consensus", + "uuid": "bcf0f03c-5951-46a7-aa38-545aed9bc183" + } + ] + } + }, + "tags": [], + "uuid": "68d6270a-bfd1-452a-abc9-4a314e13af85", + "version": 9 +} diff --git a/workflows/genome-assembly/polish-with-long-reads/CHANGELOG.md b/workflows/genome-assembly/polish-with-long-reads/CHANGELOG.md new file mode 100644 index 000000000..2d503cf69 --- /dev/null +++ b/workflows/genome-assembly/polish-with-long-reads/CHANGELOG.md @@ -0,0 +1,4 @@ +# Changelog + +## [0.1] 2023-07-15 +First release. diff --git a/workflows/genome-assembly/polish-with-long-reads/README.md b/workflows/genome-assembly/polish-with-long-reads/README.md new file mode 100644 index 000000000..a080e1458 --- /dev/null +++ b/workflows/genome-assembly/polish-with-long-reads/README.md @@ -0,0 +1,30 @@ +# Assembly polishing with Racon workflow + +## Inputs + +- Sequencing reads in format: fastq, fastq.gz, fastqsanger.gz or fastqsanger +- Genome assembly to be polished, in fasta format + +## What does the workflow do + +- After long reads have been assembled into a genome (contigs), this can be polished with the same long reads. +- This workflow uses the tool minimap2 to map the long reads back to the assembly, and then uses Racon to make polishes. +- This is repeated a further 3 times. + +In more detail: + +- minimap2 : long reads are mapped to assembly => overlaps.paf. +- overaps, long reads, assembly => Racon => polished assembly 1 +- using polished assembly 1 as input; repeat minimap2 + racon => polished assembly 2 +- using polished assembly 2 as input, repeat minimap2 + racon => polished assembly 3 +- using polished assembly 3 as input, repeat minimap2 + racon => polished assembly 4 + +## Settings + +- Run as-is or change parameters at runtime. +- For the input at "minimap settings for long reads", enter (map-pb) for PacBio reads, (map-hifi) for PacBio HiFi reads, or (map-ont) for Oxford Nanopore reads. + +## Outputs + +There is one output: the polished assembly in fasta format. + diff --git a/workflows/genome-assembly/polish-with-long-reads/test-data/assembly.fasta b/workflows/genome-assembly/polish-with-long-reads/test-data/assembly.fasta new file mode 100644 index 000000000..259cca74d --- /dev/null +++ b/workflows/genome-assembly/polish-with-long-reads/test-data/assembly.fasta @@ -0,0 +1,1395 @@ +>contig_1 +AAGTATCGCTGAACTGTGATTACTTCGTCCGGGTTTGATGAACGTGGACTTTGGCAGACG +TCGCACCGTAATGTCTGCGATGGGCTCTACGCAATGAGGGGTTCTGGCCGTTGGCGAGCG +GTTGAAGACCGTGGCGGAAGAAGCTGCTGAAAATTGGCTATGCTCTTCTCCGGCTGCTGG +AAGATGATCGACCTGTCTGGCGGCGCGGCGTGCTGGTTAACATCGACGGCGGGCTTCGGA +CCTGCGTCCTGGACGAGTTCGGATCGGTAGGTAAACACCAATCCGTGCATTCTGGCTCTC +CGACCAACGCGATCTGGGTGGTGTTATCGGGTACTTCTCTTTGACCCCGGATTATGAATG +ACGAGCTGCGCCGTTACACCGTTGGTTGCGACAGGTTATCGGCATGGACAACCTGTCCTG +AAATCACTCTGGCTGAACCAATAAGCAGGTTCAGGAGCCAAGTGATGGGATCGCTAGCCA +GCAGGCATGGGATGGCTCCGGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTTCGTGAA +TGACAGTGCCGCCGCAAACTGGGAGAAAGAGCCGGATTTACTGGGATATCCACAGGCATT +CCTGCGTAAGCGCAAGCGGATTAAGGATTTGACTGGGAATTTGGCGTTTCGAGGCTCTTT +GCGCTAAACCTGGCCCGCCGAACGTATAGTACATCATTCGGTCTGGATAGGGTTAAGTTG +GCGGAGATAATTACTGATGATCGTAAAAAAGGACAACTCAAACGTATCGTTCAGGCGACG +GGTGGTCGGTTTACATACCCGGCAAGGAAAGTGCACCCCTTGACGTTACGCCCCTGCGCT +CCGGGGCCAACACCGGGCGTCATCTATCGTCGCACCGGCACTTGACATCACACCCCGGTA +GATTTCCCGGACCGATGCCCACAAATCTTGTGCGTCGATACCCATGGCTCTGTACGTGTC +CGGTCAACGAGCATTGATGTCACGGAATTTCAAAACCGTAGAGCACCTCAACGACTGCTC +TCGCCGGGCTGGGGGCATCGATTAACATTGTTATCGAAGTTACATGCGCGCACGGAAATT +CCCCGATCTATGGACGGGGGCAAGCGCCGCTCCGTTTGTACACCCTGCTGCTTGACGGCG +GCTATCGACGAGGTTGAACCTGCGCCCAAAAAATTTCGTTCGCACCAAATGAGATGTTGT +GTCGAAGATTGGCCGATATGTGCGCTGAATTTAAGCCCGGTACAATGGTTTTCGGCTGGA +ATTTTCGACATCGTTTTTTAAACCCATTCCGGGCTATATGATCTCCCAGCAATCCAGCGC +TTAGGCTGATGTACTTCATCCGCTGATAGCGTTTTATGCGCCAGACTCAGCCGTGCGCGG +TAACGTTGGGTTTCATGGCTGTGATATGGAAATATCTGCAGTCCCGTGGTTTGTGCCGTG +GGCGGCAAAGCTTTCGATTTGTGCCCATCGTTCTGTTTGGACGAATTATCAGCCGTACTT +GAACGAAGATCGGCCTGCGTTTTGAAGAGCGAAATTTGTGCTGTCACAAAATGCTCGATG +CGATCGGGGTGACTTGTGTCATGTGTTGGTGACAATATTATTGGGTGCCATTTAACCGCT +TAATAAATCCGGGACATGTACATGAATAACAATCTAGCTGCAGGCTGCTCCTGGCGCAAA +CAGGAAGACCCTGGGAATGATGTGAACGCTTCCAGGACGAACTGCAGAACTAGCCGTATG +GCCTTCGAAAGCGCCTTTCAGCTGTACGGGCATACACGACCATTTAAGAGCTGTCGTATA +AAATTCGACTGGCAAATTCGGTGGCAGCTCTCTCCGGGCCAGGTGAACCAGTGTCGTTTT +GTTTTGTGAATTTATTATAAGAGCTATTAAAAAACGGGTTGCGAACCGCTGTTTTCTTAA +GCACTTTCCGCCACAACTTTCTCTCATTGTCCGCGTGCTGTGGACTGCAGGCTTTAATGA +TAAAGATTTGTGGCGCTAAATTACGTTCTGAAATATGATCGGGATGGCAATAACGTGCAG +TGGAATACTCGACGCCGCTGGCGACAGTTTTGGTAAAACGTCTACTTCTGGCCAGCAATC +TCTTATTTGGGATGGTTGCGGCGAGTTTAGGTTTGGCCTGCGCTCAGCAAACCCGCCGGC +GAAACCAAACGGCCCGCAAAAAGCGACAGACCCGCAACCGCACGAGCCTTCAGGCCAAAG +CTTTTAACTGTTGTGACATTGGGCCTATGCTGGAGAAGGCGAACACCACGCACGCCCGAA +TTCGAACTATTCCTGTTGAAATTACTGGCATCAACATGGCCATTCGCACACGGTAAATCC +GGCGATCATTTTCTTTTCGGCAAAATCGGCACCGCAAACACCTGCCCCGTTGCTGAAAGA +ATCTTTGCTTTCCTCAGGCCGCAACAATCTTGGCATTACTGGATACGCTCAGCTGCGCTG +CTTGACCCAGGAAGGCACGCCCGTCCTGAACAAGGGTTATCGCATTGCGATTTTATGCGG +CATTTTACCCCACATGCAAAAATTCTGCATCACGCCGGGCTGGATAAGCCAGGGGCAAGG +CATTCCGTTGCTGGGGCCCTCCAACGCCGTCACCTAACAAGCAACTTAGAACCTTTACTT +CATTATTAACTGATAACTCCGCAAGCGCGCGGGGGGGCGTTTGCGGCATTTTATTATGCC +TAATCAAAATTGTTTAACTAAAGTTTATCGGGTAGTCGTAACAGAATCGCAACCCTGCGC +CGGATTGCGCAAAGGATGGGGTCTCAACATCCATCAATGCCATGGAAACCGGGATTGGAA +AAAACTCTTCCCGGACGAAAGAACTTGAAAGGGAATAACCGCAGAAGTTTCGTGGCACGT +CTGGAAAAACGGCGAAAGTTTGCTGGGAAATCTTGATCCCCGGAAAGCGTTTCTGCCGAT +GGTTACGTGAGGCAAGTTAAGCGCGTCTTTGGTAGTGCGTCACCTTCGACGTTTCAGTTA +CTCGGGACGGTATGGTTCTTAAGAACGGCTGCATCGCCGAAATGCGGTTACCGGTGAAGG +AAAAACCACTGACCGCAACGCTGCCTGCTTAACCTGAACGCCACTAACCCGGTAAAGGGC +GTGCACACGTTAGTTACCGTCAAACGACTACCTGGGCCGCAACGTGGACGCCCGAAAACA +ACCGTCCGCTGTTTGAATTCCCTTGGCCCTGACTTGTCGGTATCAACCGCCGGGCCATGC +CAGCACCGGCAAGGCGCTGACAGCTTTAACGCAGCTGACATCACTTTACGGATATCGATA +ACAAACGAATACGGCTTTGACTACCTGCGCGACAACAATGGCGTTCAGCCCTGAAGAAAC +GTGTACAGCGTAAACTGCACTTAATGCGCTGGTGGACGAAGTGTGGAACTTCGCATTCCG +AGATCGTATGAAGCGGCGTAACACCGCTGATCCATTTCCGGCCCGGGCAGACGACCAGCT +TCGGAAATCCGTTATAAACGCGTGAATAAAATTATTCCGCACCCTGATCCGTCAAGGAAA +AAGAAGACTCCGAAACCTTCCAGGGCGAGAGGCCACTTCTCTGGGTGGACGAAAAATCTC +GCCAGGTGGAACCTGACCCGGACGGTGCGTTCTGGTGCTGGATTGAAGCAACTGGTGGTG +AAAGAGGGGCACTCATGGAATGAAGAGGGAGTTCTCTGTACCTGCTCCGGGCGCAACATC +CATAGCTCGATGGCACCACGTAACGGGCGGCGCTGCCGCGCTCATGCGGCCTTGTTTTAA +CGCGTTGACGTCGACGTACGATCGTTAAAGATTGGTGAAGTTACTCATCGTTTGACGAAC +ACACCCGGTCCGTAACCATGCAGGGGCCGTCGTCGCTGGCTCCGATGGTCTGCACCAGGG +CTTTTGTGGGAAGCGAAAGAAGGTTGTGCAAGATCCAGGAACGAAAACCAAATGCCTGGC +TTCGCATCACACCTTCCAGAACTAACTTTCCGTACTGGTATGGAAAACTGGCGGGGATAG +ACCGGTCACTGGCTGATACCGAAGCTTCCGAATTTCGTCTCAATCTACAAGCTGGATAGC +CGTCGTTGCTTCCGACCAACCCGTCCCACTGGATTTTCGTAAAGGATCTAGGCAGGACCT +GGGTCGTACATGACTGGAAGCGGCAAAAACTCAGGCGAATCATTGAGAGATATCGAAAGA +ACGTTACTGCGAAGAGGCCAGCCGGCTGCTGGGATGGGTACTATCTCACATCGGAGAAAA +TCGGAGCTGGTTGGTCAAACCGGAACTTGGCCAAAGCCGGGTATGAAGCACAACGTCCTT +AACGGCCAAACATTCCACGCCCCAACCGAATCAGCGGGATTGTTTTGCTCAGGCGACGGT +ATCCGGCTGCGGTGACTATCGCGACCATATGGCGGGGTCGATGGTACAGGATATTGCTGC +TTCGGTGGGTAGCTGGCAGAGCACGAAGTTGCCGCGGTCGGAAAGTCCGAACCGCCAGAG +TCAAATGTGAAAAAATTAAAAGCCGACGGGGCAGGTAACGTCACGATGTCGGTACTGGGA +AGCAGGTGGCCTGCATATCATCGGTCCCGGCGTCACGAATCCCGTCGATATCGATAACCA +GTTGCGCGGTCGTGCTGGGTCGTTCAGGGGGATGCGGTTCTTGCCCGTTTCTGACCTGTC +GTTGGAAGATGCGCTTGGATGCGTATTTTTGCTTCCGACCGGAGTTACCGGCATGCATGC +GTATAACTGGGTATGAAGGCCAGGACGAAAGCCATTGAAAACACCCGTGGGTGACCTAAA +GCGACTTGCCAGACGCCCCAGCCGTCACAAGTTGAAAGCCTGTAACTTCGACATTTCGTT +AAGCAACTGCTGGAAATATGATTGCACGCTGGCATAGACGATCAAAGCCGTCGCGCCCAT +TTACTCCCAGACGTTAACGGAGACTTGTTGGGATGTTCAGCGATGTGAGCCGAAAACCAT +TTAACAGCATTCGGTGAAGACTGTGGTTCAAAGCGACCAATTGATTGCCTTACATTCCTA +CCAACCAGGGTCCTGCGGAAAGCAAAATGTGGGGAATATTCCGGGGGCTGCAGGGAACGT +CTGAAGAACCGATTGTTTTCGACCTGCGCTTATGCCAAATTGCCGAGTGGCTTGGATTAA +AGAATACCAGAACTAGCGATGAAGAGACGCTCGCTGAGCGCATTCTGGCTGCAAGTCCTT +CGGAAGTGGTATCAGCGTAACAGAAGAAGTGGTTGGTGCTGAGATGATGCGGTCACTTCG +AGAAAGGCGTCAATTGCTGGCAAACGCTTGACTCCCTTGGAAAAAGAGCACCTGGCAGCC +GGTGGCCTATCTGCGTCAGGGTTACCACCTGGCAGTTGGGCTACTGGCACCAGTTGAAGA +ATCCGAAAGCAGGAATCCATAGACGTTAGCAATCCCGTTGTCCATGTTTTGTCAGTCGAT +GCTGGAGAAGTCGTTGAAAATATGATAGTTAGCACGGTACGCCTGAGCAAAGTTCCAGGT +ACGATATGCCTGAGAAGCAGGTTGAGGAAGCTGGAACAACAGGCGTCGTAATCGGAAGCC +GAGCGTTTAGCGCAATTGCAGCAGGCTTAGCCCATCAGGATGACGCACTCTGCTAGCCCG +GCCAGCTGCACTGGCGGCGCAAACACTGGAGAGCGCAAAGTAGGAACGTTAACGGATCCA +TTTGCCCGTGCGGTTTGGGTAAAAAATACAAGCAGGTGCCATGGCCGCCTCGGCAAATAA +AAGCTTAACTGTTGAAGTAAAAGGCGCAGGAATTCTGCGCCTTTTTTAGAGGTTTAAGAG +CAATGAAAAAGAGCTGCAAATTGCGGTCAGGTATTGATTTCGCAACGAGAACAATGAAAA +TCTTTCATAAATCGCGTCGCGCAAAGCAGATGCGCACATGGCCGAAATTAAACTGGAGTT +TCCCGGGCGGTAAAATTTGAAATTGGGTGAAAACGCCGGAACAGGGCGGTGGGTGGCGAT +GAACTTCAGGAAGAAAGTCGGGATGCTAGCCCCCCAAGATTTTAGTCGCTTATTTGAAAA +ACCTGGAATATGAAGTTCCCGCGACAGGGCATATAACTAGTGGTGGTTTTGGCCTGGTCC +GAAACGCTGGGGAAAGGGGAAGCCGTGGGTGTAAAGAAGGGCAACCCGGTGTGACGGTGG +ATGTCGGCTGGATCTGGTCTTAATGGCCAGATGATTTTTCCGCCCAGCCAATGAAACCGC +GTAATTGCGAAGCTTAAAACGGTCTCGTAGGTCAGATAAGCGCGTTTTCGCCGCATTCCG +ACTATTCGCTACAACGATGCCTGATGGCGCACGCTGTGCGGGTCTTATCAGGCCTAAAGG +ATTTCTAACTTCATTGATAAATATTGTTTTTTGTAGGTCGGATAAGGCGTTCACGCCGGC +ATCCGACATTTGCACAAGATGCCTGATAGCGACGCTGTCGCGTCTTATCAGGCCTACGTG +CGGCATCAGACAAAATGTCACCTGCTTTGGTTCTTCGCTCCAGTCATCGCTTTACGGAAA +GATCGCCACTGCTGGGGATTCCGTTTTTTCCTTCAGCAGCCCCATATCTCCGAGGTCCGA +TCAGCTGACAAACGTTTGGAGCAAAATGGCCGAAACGGGCTGATTTTCACCCCACACCAC +CGTTTTCCCGCAGGTTGGGCAATTCACCCGTAATAGTTTTCTGACATTTTTACTCCTTAG +CAACAGGCCAGTTCGAAAATCCCAGACGTTCTCCGGTACCTGTCCGTCTTTCAGTGTCCA +GCGGCATAAAGAACGAAATGGCAGAAACTGGCTCTTATGTCCGGATAATTTGCGGATAAA +GCTGTGAATCGAGCGACAGATTCAGGCGCAGCAAGTCCGGCATCCCACCGTTATCCTGAT +AAAAACCATTCAGGCTGGTTTGTTTACGGAAGGGGGGCCGACTGGCGAATTAAATCCCAG +CACCATGGTAAGTGCCTGGGTGAGCGGGTTCTAGGGCTGGCAATCCAGGTTTCTAACCTG +GCTGTCGCGCTTGCGCCTGGGGTAGGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAG +CTGCAACAGCCGCCTGGGATGCCTCAGTCGCTGACGCACCACAGAAGCCAATCAAACGAT +CTCACGCAGAAATTGCCCGGATACGCGGCGCGGAAATTAAATACGCTCCCGCCGCCGTTT +TAACACTGCTAGAATTAATGCTTCAATACGGCTCTGGTCCACGCCAGGCCACGCCAAATC +CAGGTCTGGGAGTTTACGTTGCTGCCGGTCAAGGTTCTTTCAACAGCTCAGTGCGAGACT +TCGGCCGGCGCTCGAAAACATCCAGTAATTCACTGACATTACGGAAGAAAATGAGCAGCG +CGCCAGCGTGGTCAAACGATGGGGTAAATTTAACGGTGAGGTTGCTGAAGTCAAAAACTC +AATGCGCAGCCATGGTACGCATTTTTTCATTTAGTGGATGTTCAAAAAAGGACCTGGGTC +TGCACTTACGGTTTTTCCTGTGAGACAAACTGCGGGACGCAAGCCTGCAAATAGTGTGCG +TGCAGGCGGGCAACATCCGGATGCAGATAGCATCCGGTGCGCCGTTATTATCAATGACGT +CATCTGCCACGGCAAGGCGGGCTTCGCGCGTTGCCCTGAGCAGCGAAGGATTTTGGTTCC +GACATGCTCGCGAGTTACATCATCGCGCTGCATGGGTGCGCTTAAAGTTGCGTTTCTGGG +CTGACATCCAACCACAAGCACTCGAGTTCGCTTTTTTTATACAGTGAGTTTTCTACCCAG +CAATGGCACAACCCACAGTACATAGGGGGAGAGTAGCTTGCTGGATCTGGGTGTTGGCGT +CTTCTTGCTGAATCAGCGGATGCAGCAGGGCGTTAAGCCAGGTATTCTTCTTTCCGGGTT +GGCGAAGATCCGCTCGCGCAAGGCCCGGGCGCTGCAATGTTCCCATCTAGCAAGCAATCA +TGTTAGCGCCAAAGTGATCAGCCACAGGCATGTAGCGCAAGGTGCACCTGGTTTCAACCA +CCTGGACGGCGCAATAATATATCGGCATCAATGACGTTAATTCCGAGGATCAGCAAACGC +ATTGGCAACGGTACTCTTGCCACTGCCAATGCCTCCCGTTAAGGCAACTATATACCTCAT +AATTTATAATTCCCGGGAATTCAGTCAGTGATTATCCAAAACGTGTTAAAAATGAGTGCA +CGAAAGCGAAATTGATGAAACGTTCGCTCACTATCTTACCAGGTAAATTTTATGGGATTG +TAGCGTAAAAAAAGACAATTTCGCAGTCTTGCGCCGCACTTGAGTTAGTGCGTATGATAG +CGTCACTGGAGTTGCCGCTCTTACCCTTATTAGCCATTAACCCCCAGGAATCCCGCACAT +GGCGTATTGAAAGGAAGATCTGAAGTTAGGTTTTAAAGACGTTCTCATCCGCCCTAAACG +CTCCACTCTTAAAAGCGCTTCGCGATGTTGAACTGGAACGTCAATTCACCTTCACAACAT +TCAGGTCAGAGCTGGTCCGGCGTGCCGATTATCGGCCGCAAATATGGGACACCGGTAGGC +ACATTTTCTATGGCCTTCTGCGCTGGCTTCCTCTTTGCATATTTTTGACTGCTGTGCATA +AACACTATTCTGTCGAAGAGTGGCAAGCGTTTATCTCAACAAATTCTTCCCGCTGATGTG +CTGAAAACATGTGATGGTTTCTACCGGTCACGTCTGATGCGGGATTTCGTAAAAAACTAA +ACAGATTCTCGACCTGAACCCGGCATTAAACTTCGTTTGTATTGACGTGGCGAATGGTTA +TTCCGAAACACTTCGTGCAGTTCGTTGCGAAAAGCGCGTGAAGCGTGGCCGACCAAAACA +CCATTTTGTGCTGGTAAACGTAGTGACTGGTGAAATGTGTGAGGAGCTTATCCTCTCAGG +TGCCGATATCCGTTAAAGTTGGCATTGGGCCACAGGTTCTGTTTGTACAACTCGCGTCAA +AAACAGGCGTCGGTTATCCGCAACTTCTCTGCCGGTAATCGAATTGTGCCGATGCTGCGC +ACGGTCCTGGGCGGAATGATCGTCAGCGATGGTGGCTTGCACCCACGCCGGGGCGATGTG +GCGAAAGCCTTTGGCGGCGGTGCCGATTTCGTCATGCTTTGGCGGCATGCTGGCGGGGCC +ACGAAGAGAGCGGCGGTGCGCATCGTTGAGGAGAAACGGCGAGTAATTTTATGCTGTTTC +TACGGCATGAGCCTCCGAGTCCTGCGAGGTGAAACGTCACGTTGGCGGCGTTGCGGAAAT +ATCGCGCAGGCAGAAGGTATAAACCGTTAAGCTGCCGCTGCGAGAGGCCCCGGGTTGAAA +ATACCGCGCGAGATATTTTGGGGGCGGCCTGCGTTCAGCTTGTACATACGTTGGGGCTTC +ACGCCTGAAAAGAGCTGACCAAGCGCACCACCGTTTATATTCGTGTGCAGGGAACAAAGA +AAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGT +TGATCACCATCCCACCTCATTCGCATCGCCTAAATGGAAAAATTGGCAAGATACATTGCC +ACCACCACGCGGTACCAATAATTCCTCCCCGTTATGATCAGCAACGCCGGTTCCAGGTAA +GGCTGCGAGGTTATCCGCCAGCGCCATTGTGTTTTCCCCGATGATGATGGGCGAGGTTGT +CTAACATGAGATCCCAGAGAGCCGGATGCCTCTCCTGTTCTCACTAATTTGCAAAACAGG +AGCGGGCTAAACTCACCCGGTATTTTTATAGCGCCAGCCCAGATGGGTTGACCGTTGACT +GATATCCGTGCTGGATTTGTGTCAAGAAGTTGCACCCCAGTACGGGCAGCGCATTGTTTT +CTCTGACGCTCTCTACGCCCTGTAAAAAAGTAATGCCCTGCACTTTGTGTCAGCGCCAGA +ATCGTAAAGATCTGCGTGAGTTTTTTGTCCCCGCATCAGTGAACCCATAATCGGGATGCG +TAACAGCAATATTCTGCCGCACTATAAGCCAGGTCGGTCGGCGCATCAGCAACTTATTGG +CTATCGGCCAGCAGAAAGCCGAACAACACCCAGCAGCCAGCTCCATTCGCCACTAAAGTC +TGCCAGCGTCATGATCCCCTGCGTTAGTGCCGGTAGTGGGGGTGTTGAAGGTCTTATAGA +TAGCGGCAAACTTCCGGCAGAACAAAAATGCAGCATTGCCACAACCACCATGATTGCCAT +CGCTAAAATGATGATGGGGATAACGTAACGCTGAATTTCACTTTGTCGGGTCAAGCTGAC +GCTGGGGCTTTTTTGCTGAACGCGCCAGTTCAAAGCAGCATTCATCCAGCTTACCGGTCA +GTTCCACCCGTGCGGATCATCGCCTGATAGAGCGGCGGAAATACCTCTGACCAGGGTAAT +AAGGCATTGGAAAAAGCAATGCCCTGTTCGAGATCGTGCGCCAAGCGATTGCAGCAACGC +TTGCCATTGCTTACTGGGATGCTGTTCCGCCAGCAGAGCCAGCCCTTCAGAAAGCGTTAA +CCCTGCTTTGAGTAGCGTCGCCCAGTTGATGAATGACTTCCGCGCTTTTATCTCCTCGCC +ACTGCGCAGAATTGATGGCGATTCGCTTCAGGCTTAGTGGGGTAACCATCTGTTGCTGTA +GTGCCATTAAGCAGTAAAGTACGGCTCTCTGCCCATAGCATCCCATCTTGCGCATTGCCG +TCGCCGGTTATTGCCATGCCAGCGCCAGAGTTGCTTACTCTCGCCATGCGGCATCCCCAG +TACGCGGGATTAACTCTTCAAAGGTGGTTAAGCCTTGCTCTACGGCCAGGCAGCCCGTTT +TCAAAAAGCGTACGCATACCCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCAACGTCGGT +ATTAGCGGAAATAAGCTGACGAATGACCGGCGTTATGGGCAGAACTTCAAATAAGGCCGT +ACGACCATAAAAACCGTGGTAGCAATGTTACACAACCGGGTGCCTGCCCAGTGGGGCAGC +GGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACAATGT +GGGCAAAGTTTTGCGTACCAGACGCGTGGGCTATTACCAGCGTAAGCGCCGATGATAGCA +TCCAGCGGGGCGACCCCCATTTGCTGTAAACGTACCAGCGTTTCGCAGGTGGAAATTTAG +TGTGTAGGGTAGACAACACCAGGGTGACCAGTTTGCGCCGCTTTAATAGCGAATCTCTGC +TGTTTTCGCCATCGCGGATCTCTCCGATCATGGATGACGTCAGGATCCTGGCGCAATAAC +GCACGCAAAACGCCCTGAAAGGTGAGTCCGGCACGCGGATGGATTTGCGTCTGGTTTAGT +CCGGCTATGGGGATCTCAACTGGATCTTCGACGCTACAAATATTAATTGTCAGCGGTATT +CAGCTTTTGCAGGGCACTATAAAGCGTGACCGTTTTGCCGCTGCCCGGTAGGGCCAGTTA +CCAGCACCAGTCCCTGTGGTTGTTGCAAGGCATGAGCAAAGTCCGCCAGTTGTAACGGCT +GCATTCCAAGCGTGTTGACATCCAGTGCCTGACCCACCTTGCTGTAACAACCTTAATACC +ACCTTTTCACCACCCCGACATGGTAAGGTCGCAATACGAAAATGAGACGGCGTTTCCTGC +CAGTTCGACAGTGAATTGCCCCGTCCTGCGGCAGGGCGATGTTCCGCAATATCCAGGTTT +CCCAGCAACTTTTAATCTGGCGGTTAATGCGACTCCGGCATCCGGTGAAACATCCGGTAA +AGGATGCAATACGCCGTCGATACGCAAGCGGATGCGGTAGGCATTGTCCGCTGGTTCGAT +ATGAATATCAGACGCGCGTTGTTCCAGCGCAGATTGTAACGTTCGAGTTTAGCAACTCTG +CTTTTGGGCTGATGCTTCTCCTGAACAGCTACGGGCAATGTCTGTTGTGTGCGACTGGCG +TGGACCTTCCATTTGTTGGCGCGTCCAGCAGGTGATCTCAATACGTTTGGTGGTAGCGAA +ATGCAATGCGTCCAGTAGGCTCATGCGAAGGTGCATCGACTACCGCAACAATGAACCACC +TTCTTCGCTGGCATCCAGCAAGACTCCATTGATAACGCAGACACAGGGGCAGTGAGCTGT +GGAATATTCATTGCCGCTCCTTAGTTGGCGTCATCAAAGCGGAAGACATCTTCGCAGGCT +TGCTGCAATTGCGCTGTCACTTTGAATTATTGCAGTTTGCGCGTCCAGCCGGTGACGCCG +TTTGCGTTATCCCAACCCGGTGTCATGACGACGCTTAGCCCATTGAGACTTTCTTGCCCG +GTCAGCGACAACCACGCCCCTTTGCCACACTCATGGCTGAAACATAGCGGGTTGGTGGTA +GGCGAGGGAATGCCAATTGCTTGCCACCGTCGCAGGGTATCTAATCCACACCATGTTCCA +GCGCGCACAACTCTACGGCGGTACGGTAAGGCACAAAAGGTTTGTAGCATGTCGGGTGAG +TGCGGCTTTGCGCAGGTAGTTTTTGATAAGCGGGAATACCAATGGCGCTTAAAATGGCAA +TGATGCCAATAACCACCATCAGTTCGATAAAGTGTAAAACCGCGTTGCTTGTCCCATCTG +TTTTCGCTCCTTGATTTGGATTGGCGCTACTTTGGCAAAACGGCCATCAGCACAGGCGAG +GGGGCAAAAAACGAAACGAGGGAAAGCAGATTCCGAGGTTTTTTATTTCGTTGCAGCGAA +AGACAAGAAATTTGCGAGGCGTTTACGAAGAAAGTTGGGGAAGGGGGAGATTATCCGCCC +GCGATGGAGCGGATAAATCTGTTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCT +TGTACGTGTTTAGTTAGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCA +ACGCAGTGTTTTGTCAGTGAACGTTGGCCAGACACTTCCAGTAGCGCCTTGCCGTTGGTG +CGTTTTGACGGCTTCGCGCATCTGTTCTGTTTCGAAGTTATCCAGCATGATGATAATCGG +CTCCTGCTTTGCAGGGCTTTCATCAAGTTCTTCCAAGATTCTTCTACTTCGACTTCTACT +GGCGCATCCGGGTGCAGCCAGGACGCGTTTTTTCGACCGCCTGGCGCACTGAGCCCGGAG +GCAATAATATGGTTTTCTTTGATCAGGAAGGCATCAGAAAGCCCCCAGACGGTGATTCGC +TCCGCCGCCGCAAAGTACCGCGTATTTCAGAGCTGAACGCAGGCCGGGTAAGGTTTTGCG +CGTATCCAACAACACTGCGTGTTGGTGCCTTCCAGCAATTTCGACATAGTGGCGTACCTT +ACTGGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCCCGTTAACA +GCACGCGGGATGGGCCTTCAAGTTCGAACAAGGATTGATTGGGCATTGATGACATCGCCG +TCATCCACATGCCAGATTATGGTGACATCGTCGCCTGCCAGTTGAATAAACACCTCTTCA +ACCCAGCGTTTGCCGCAAAAGACGCCATTCTCCGCGGGTGATCACCGTGGCATGAGAGCG +AGAATTTTTCCGGTAAAAGTTTTGCCGTAAATATCATTGTTGGCATCGACTGTTCCGCCT +AAATCTTCCCGCCAGCGCCTGGGCCACCGCGCCGGGGATATCGAGATTAATGCGTTCCAG +CAGCTCGTCACGTCGGGTGTCAGGGTTATAGCGGCGAGGCGGCATGTTAAAACTCCAGAT +AGCTAACGAATCATAAGGTAGAAACATGCTACTCTGAACCGGGTATTAGCACCACATATA +AGGAGATCCTGCATGTTGTTAGAACAGGGGGTGGCTGGTTGGCGCGCGCCGCGTTCCCTC +ACCACATTACGATTGCCGCCCGGATGACGAAACACCCACCCTGCTGGTGGTGCACAATAT +TAGCCTGCCGCCAGGCGAGTTTGGCGGTCCGTGGATCGACGCATTATTCACTGGAACTAT +TGATCCCGCAGGCACATCCTTTCTTTTGCTGAGATCGCCCATTTGCGCGTCTCCGCTCAC +TGTTTGATTCGCCGTGATGGTGAAATAGTTCCAGTATGTTCCTTTCGATAAACGTGCATG +GCATGCGGGAGTCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTATTGGGAT +TGAGCTTGAAGGCACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGC +GGTTACGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATTG +TGATATTGCGCCGGATCGGAAAAACCGATCCCCGGTCCCTGCATTTGATTGGGCACGGTT +TCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTTAAT +TTTCGAGCGCCTGTTTAAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGT +TCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGGCATGACCATTATTGCGA +TGGGCGTGACTTTTTTTACTGTTACGCGCATTGCAGGGGAGTATTGTTCAACGTTCCCCA +CGCTACTGGTGTGGCTGCTGATTGGTTTGCTGGTGTATTGGCGCAGGTAAAGTTCGTCTT +CATTATCAATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCCCGTGCCACCGA +TGGCTGGCGAACTCACCATGATTCACGGCGTCCCGGCAGGCTGCGACGAAACGTGAGTAT +TTGCGTGAGCTGCAAAATGGCATTGCTGTGGATTTAACTTTCGTTTTTATCTTGCACCGC +TGTTCCTGGCTGATTGTGGGGGGGAACCTGGGGACCCGTTACGCTGATGGGGGTATGCGT +TTTTGCGTGCATGGCAATACTGGCTGGCGCGGATATCAGACGCCGCATCATCGGTTTAGC +AGTCCGGCATTGATGCCGTGCTTCATGTACTGGATTGGGTGCCGGTTCGTCTTGCGGGTG +TGGTATATGCCTTGATCGGTCATGGTGAGAAAGCGTTACCGGCCTGGTTTGCTTCGCTGG +GGTGATTTCCATACTTCGCAGTATCAGGTGTTAACGCGTCTGGCGCAGTTCTCTCCTGGC +GCGTGAACCGCATGTCGATAAGGTGGAGACGCCGAAGGCAGCGGTTTCAATGGCGAAGAA +AACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTTACGGGGCGTTTGGTT +GTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTA +CGGCTTTGGCGGTTTTCTCTTTAAACAGGATAGCCGATACCTAACACGATCAGCCATACC +GGGATCAGGTATACCGAAAATCGCCATTCCTGGGGTCATCAGCATAATCACCAGTACCGC +CGCCATAAACAGCAGGCAGATCCAGTTACCCAGCGGATAAAAGCAGAGCAGGGAAGCGAG +TTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCAATC +GCCCAGCTTGATTACCAGTGCAGATACCACCAGCGCCATTAACAGTCCGAAAGCGGACTC +TGGGGCAAGGTAGTTAATCAGTACGCACAACGCCGTTACCAGTGCAGACACCAGAATGGT +ATTTACTGGTTACACCACGTTTATCGACAGACGCCAGCGCTTTTGGGCGCATTACCCTGT +TGTGCCAGACCAAACAGCATATCGGCTGTTGCAATATACGCAGCTGTTGTACACGGAGAA +GCGCCGCAGTCAGTAACCACGATGTTCAGCGCATTCGCCACAAAGGTATCGCCTAACTCG +TGGAAGATCAGCACAAACGGACTGGTATCGGCGGTAACGCGGGGTCCACGGCATCAGTGA +GAGCAGAACGGCTAACGGAACCAATATAGAAAATCAGGATGCGGTAGATAACCTGGTTAG +TTGCTTTCGGTATACTTTGCTCCCGGGTTATCAGCTTCTGCTGCGGTGATCCCCACCAGT +TCCAGACCACCGAACGAGAACATGATAATCGCCATCATCATCACCAGCCCGGTGAAGCCC +GTGCGGCAGGAAACCACCCTGATCCCACAGGTTGCTAACGGTCGCCTGCGGGCCGCCGTT +GCCACTGAATAAGCAGCCAGCGCCGAAGATGATCATCGCTACCACCGCGATAACTTTGAT +AATGGCAAACCAGAACTCCATCTCGCCAAACACTTTAACGTTGGTCAGGGTTGATTGGCG +TTAATCACCACAAAGAATACGGCGGCAGAAACCCCAGGTGGGGATTTCCGGAATACCCAG +AACTGAATGTATTTACCCACGGCAGTCAGCTCAGCCATGGCAACTAAAACCGTACAGTAC +CCCAGTAGTTCCAGCCAGAGGCCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAA +GTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATCTCACCCAGCTGACGCATGAT +CAGAAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGATAATCCCTGGCCCTGCGGACT +GTATTACGGAGGCCGCTACCCAGGAATAACCCGGTCCCTTATCGCGCCACCCAGCGCGGA +TAAGCTGAATATGGCGGTTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCT +TCCATCATGAAACCTCGTGCGGTGGTTGTTTTTTTTGATCTACGCAGTGATGCGTGTGTA +AGTTTGCAATATTCCGTTTGTTGTATTAATTTGTTTTACATCAAAGAAGTTTGAATTGTT +TACAAAAAGACCTTCCGTCAGATCAAGAATAATGGTATGCGGCAGCGCAATGCACCCGCT +TTATGCATGGTTGAAGATGAGTTGCTTAAAAAAGAAAACCCGTTTTGTAAAGCTCAGCCT +CAACCCCTCTCAATATGTAGAATGAAATTTAAAATTCGTTTTAATTGAAATTAAAAATCA +CAAAATTGGTAAGTGAATCGGTTTCAATTCGGACTTTTTATAGTTTAGATAATACGTTAA +AAAATCATCCTTTCCCTACGTAAATGTCTACATTTTGTGCATAGTTACAACTTTGAAACG +TTAATATATGTCAAGTTGTTTAAAATGTGCACAGTTTCATGATTTCAATCAAAACCTGGT +ATGGACATAAGGTGAATACTTTGTTACTTTAGCGTCACAGACATGAAATTGGTAAGACCA +ACTTGACTTTCGGCAAGTGGCTTAAGACAGGAACTCATGGGCCTACAGCAAAATCCGCCC +AACCAAAACTCTTCCGATGTGATTTGAGCAGCAACCTGGAGTTTTTGATGCCTCGAAGGC +ACTCCTCCGCCCGGGCGAAAAACTCCACACCGGAACGCGAACTGGCAAAAACAGTTTGAC +GTCTCCCGGTCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCCGAAGGGCTTGTGTT +GCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGGCAGCCTATGGCAAAGCTTCAGCG +ATCCGCTGGTGGAGCTGCTCTCCGACCATCCCTGAGTCACAAGTTATGACTTGCTCGAAA +CACGACACGCCCCTGGAAGGTATCGCCGCTTATTACGCGCCGCGCTGCGTAGTACCGATG +AAGACAAGGAACGCCATCCGTGAACTCCACCACGCCATAGAGCTGGGCGCCAGCAGTCTG +GCGCGATCTGGACGCGGAATCAAACGCCGTACTCCAGTATCAGATTGCCGTCACCGAAGC +GGCCCCCACAAGTGTGTTCTGCTTCATCTGCTAAGGTGTATGGAGCCGATGTTGGCCCAG +AATGTCCGCCAGAAACTTCGAATTGCTCTATTCGCGTCGCGAGATGCTAGCCCGCTGGTG +AAGTAGTCACCGCACCCCGCATATTTTGAAGCGATTATGGCCGGTAAGCCGGAGGAAGCG +CGCGAAGCATCGGCATCGCCCATCTGGCCTTTATCGAAGAGAAATTTTGCTCGACAGAAG +TCGTGAAGAGAGCCGCCCGTGAGCGTTCTCTGCGTCGTCTGGAGCAACGAAGAATTAAGA +TGATTTTTCTGGGTAAAAATTATCCAGAAGATGTTGTAAATCAAGCGCATATAAAACGCG +CGGCAACTAAACGTAGAACCTGTCTTTATTGAGCTTTCCGGCGAGGAGTTCAATGGGGAC +AGGTTCCCAGAAACTCAACGTTATTAGATAGATAAGGAATAACCCACAGTCAGAACGTTT +CCCCAAATGACGTGGAATCCGATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGGT +CATCCCGTGAAGAAGGTGTTGAGCGTGCTCAGTACTCTGATCGACCAACGTGCTTTGCTG +AAAGCCCCGCAAAGGCGGTGTATAACGTAGCCGCAGGCACAGGTATCAGCAACTACATCA +ACACCATCCCCGTTGAAGAACAACCGGAGTATCCGGGTTAACTCTGGAACCTGGAACGCC +GTATTTCGTTTCAGCTATCCGCTGGAAACGCCATCATGACGGTGCTGCGTGCGTCGAAAA +AAGACCTCGAACTGGGCGGCCATATGGCGTCCTTCCCAAGTCTTCCGCCAACCCATTTAT +GATGTGTGCTTTTAACCACTTTCTTCCGGTGCACGCAACGAGCAGGATGGCGGCGACCTG +GTTTACTTCCAGGGCCACACATTCTCCCCGGGCGTGTACGCTCGTGCTTTCCTGGAAGGT +CGTCTGACTCACGGAGCAGCTGGATAACTTCCGTCCAGGAAGTTACACGGCAATGGCCTC +TCTTTCCTATCCGCACCCGAAACTGATGCCGGAAATTCTGGCAGTTCCCGACCGTATCTA +TGGGTCTGGGTCCGATTGGTGCTATTTACCAGGCTAAATCCTGAAATATCTGGAACACCG +TGGCTCTGAAAGATACCGTCTAAACAAACCGTTTACGCGCGTTCCTCGGGTGACGGGTGA +AATGGACGAACCGGAATCCAAAGGTGCGATCACCATCGCTACCCGTGAAAAACTGGATAA +CCTGGTCTTCGTTATCAACTTGTAACCTGCAGCGTCTTGACGGGCCCGGTCACCCGGTAA +CGGCAAGATCATCAACGAACTTGGAAGGCATTCTTCGAAGGTGCTGGCTGGAACGTGATC +AAAGTGATGTGGGGTAGCCGTTCGGGATGAACTGCTGCGTAAGGATACGCAGCCGGTAAA +CTGATCCAGCTGATGAAGAAACCGTTGGACGGCGACTACCAGACCTTCAATCGAAAGATG +GTAGCGTACGTCCGTGAACCACTTCTTCGGTAAATATCCCTGACAAACCGCAGCACTGGT +TGCAGACTGGACTGACGAAGCAGATCTGGGCACTGAACCGTGGTGGTCACGATCCGAAGA +AAACTCTACGCCTGCATTCAAGAAAGCGCCAGGAAACCAAGGAGCAAAGCGACAGTAATC +CTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCG +CACCAGGTTAAAGAAAATGAACATGGACGGTGTGCGTCTATATCCGCGACCGTTTCAATG +TGCCGGTAGTCTGATGCAGATATCGAAAAAACTGCCGTACATCACCTTCCCGGAAGGTTC +TGAAGAGCATACCCTATCTGCACCGCTACCAGCGTCAGGAAACTGCACGGTTATCTGCCA +AGCCGTCCAGCCGAACTTCACCGAGAAGCTTGCAGCTGCCGAGCCTGCAAGACTTCGGCG +CGCTGTTGGAAAGAGCAGAGCAAAGAGATCTCTACCCACTATCGGCTTTCGTTCGTGCTC +TGAAACGTGATGCTGAAGAACAAGTCGATCAAAGATCGTCTGGTACCGATCCATCGCCGA +CGAAGCGCGTACTTTCGGTATGGAAGCGTCTGTTTCCGTCAGAATTGGTATTTACAGCCC +GAACGGTCAGCAGTACACCCCGGCAGGACCGCGAGCAGGTTGCTTACTATAAAGAAGACG +AGAAAGGTCAGATTCTGCAGGAAGGGATCAACGAGCTGGGCGCAGGTTGTTCCTGGCTGG +CAGCGGGCGAACCTCTCTTACAGCACCCAACAATCTGCCGATGATCCCGTTCTACATCTA +TTAACTCGATGTTCGGGGCTTCCAGCGTATTGGGCGATCTGTGCTGGGCGGCTGGCGACC +AGCCAAGCGCGTGGCTTCCCTGATCGGCCGGTTAACTTCCGGGTCGTACCACCCTGAACG +GCGAAAGGTCTGCAGCACGAAGATGGTCACAGCCACATTCAGTCGCTGACTATCCCGAAC +TGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCCATCATGCATGACGGTCT +GGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACATCACTACGCTGAACGA +AAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAAGGAAGGTATCCGTAAAGGTATCT +ACAAACTCGAAACTCATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGGCTTCCGGTT +CTATCCTGCGTCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCCGTAGGT +TCTGACGTTTTATAGCGTGAACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTG +AACGCTGGAACATGCTGCACCCGCTGGGAAACTCCGCGCGTTTCCGTATATCGCTCAGGT +GATGAACGACGCTCCGGCAGTGGCATCTATCCGAACTATAATGAAACTGTTCGCCTGAGC +AGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTAACTGATTGGCTTCGG +TCGTTCCCGACAGCCGTGAGAACCTGCGTCACCACTTCGAAGTTGATGCTTCTTATGTCG +TGGTTGCCCGGCGCTGGGCGAACTGGCTAAACGTGGCGAAATCGATAAGAAAAGTGGTTG +CTGACGCAAATCGCCAAATTCACACATCGATGCAGATAAAGTTAACCCCGGCGTCTGGCG +GTAAGAGGTAAAAGAATAATGGCTATCGAAAGTCAAAAGTACCGGACATCGGGGCTGATG +AAGTTGAAATCACCGAGATCCTGGGTCAAAGTGGGCGACAAAGTTGAAGCCGAAACAGTC +GCTGGATCACCGTAGAAGGCGACAAAAGCCCTCTATGGAAAGTTCCGTCTCCGCAGAGCG +GGATATCGTTAAAGAGGATCAAAGTCTCTGTTGGGCGATAAAAACCCAGAACCGGCGCAC +TTGATTAATGGATTTTCGATTCCGCCGACGGTGCAGCAGACGCTGCACCCTGCTTCAGGC +AGAAGAGAAAGAAAAGAAGCAGCTCCGGCAGCAGCACCAGCCGGCTGCGGCGGCAAAAAG +ACGTTAACGTTCCGGATATCGGCAGCGACGAGTTGAAGTGACCGAAATCCTGGTGAAAGT +TGCGCGATAAAGTTGAAGCTGAACAGTCGCCTGATCAACCCGTAGAAGGCGACAAGGCTT +CTTTGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTG +ACAAAGGTGTCTAACCGGCTCGCTGATTATGGTCTTCGAAGTCGCGGGTGAAGCAGGCGC +GGCAGCTTCCGGCCGCTAACAGGAAGCAGGCTCCGGGCAGCGGCCCCTGCACCAGCGGCT +GGCGTGAAAGAAGTTAACGTTCCGGATATCGGCGGTGACGAAGTTTGAAGTGACTGAAGT +GATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACCTGATCACCGTAGAAGGCG +ACAAAGCTTCTATGGGAAGTTCCGGCGCCGTTTGCAGGCGTCGTGAAGGAACTGAAAGTC +AACGTTCGGGCGATAAAGTGAAAACTGGGCTCGCTGATTATGATCTTCGAAGTTGAAGGC +GCAGCGCCTGCGGCAGCTCCCTGCGAAGAACAGGAAGCGGCAGCGCCGGCACCGGCAGCA +AAAGCCTGAAGCCCCGGCAGCAGCACCCAGCTGCGAAAGCGGAAGGCAAATCCTGAAATT +TGCTGAAAGACGACGCTTATGCTTCACGCGACTCCGGCTGATCCGCCGTCTGGCACGCGA +GTTTGGGTGTTAACCCTTGCGAAAGTGAAGGGCACTAGGCCGTAAAGGTCGTATCCTGCG +CGAAGACGTTCAGGCTTACGGTGAAAGAAGCTATCAAACGGTGCAGAAGCAGCTCCGGGC +AGGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGT +TTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCCGAACCTG +AGCCGTAACTGGGGTAATGATCCGCATGTTACTCACTTTCGACAAAACCGATATCACCGA +GTTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCGGCGAAACGTAAGCTGGATGTGA +AGATCACCCCGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCTC +GCTATCGAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAAGAAATACATCAA +CATCGGTGGTGGCGGTGGAGACCCCGGAACGGTCCTGGTTGTTCCGGTATTCAAAGGACG +TCAACAAGAAAGGCATCATCGAGCTGTCTTGCGAGCTGATGACTATTTCTAAGGAAAGCG +CGTGACGGTAAGCTGACTGCGGGCGAAATGCAGGGCGGTTGCTTCACCATCTCCAGCATC +GGCGGCCTGGGTACTACCCCACTTCGCGCCGATTCGTGAAACGCGCCGGAAGTGGCTATC +CTCGGCGTTTCCAAGTCCGCGATGGAGCCGGGTGTGGAATGGTTAAGAGTTCGTGGCCGC +GTCTGATGCTGCCGATTTCTCTCTCTCCTTCGACCACCGCGTGATCGACGGTGCTGATGG +TGCCCGTTTCATATACCATCATTAACAACAACGCTGTCTGACGTTCGCCGCTTGGTGATG +TAAGTAAAAGGAGCCCGGCCCAACGGCCGGCTTTTTTCTGGTAATTCTCATGAATGTAAT +TGAGCGTTACATTAGCGAAATAGACAAATCGGTTGGCCGTTTGTTGTTTAAAAATTGTTA +ACAAATTTTGTTAAAATTACCGACGGATTAAGAACGACCCCGGTGGGTGGGTAGGGTAGT +ACCTTCACCATTACCCTATGGATTTTCTGTGGTGCAGGAAGGTAGCCAAGCGCCCAGAAT +CCCCCAGGATGCCTTACATAAGTAAGTGTAACCTGGGGTGAAGGGCGTGAAGCTAACGCC +GCTGCGGGCCTGAAAGAACGAACGGGTATGAACCGCCGGAGATAAATATAATAAGAGGGT +CATCGAATGAGTTCTGAAATCAAAAACTCAGGTTCCGTGGTTACTTGGGGGCAGGGCCCG +CAGGTTACTCCGCTGCCCTCCGTTGCTGGCTGCATTTTAGGTCTGGTAAACCGTAAATCG +TAGAACGCTACAACACCCTTTGGCGGTGTTTTGCCGGAAGCGTCGGCATGTATCCCTTCT +AAAGCAGCTGCTGCGACGTAGCAACAAGCTGTCGAAGAAGTCCAAAGCGGCTGGGTCTGA +ACACCGGTTATCGTCTTCGCGAACCGAAAACCGATATCGTAGCAGATTCGTACCCTGGTA +AGCAGACACGTGCATCAATCAGGCTGACCGGTGGTCTGGCTGGTATGGGCGAAACGCCGG +CGCAAAGGTCAAACGTGGTTCACGGTTCGAGTCTGGGGTAAATTCACCAGGGGCTAACAC +CCTGGAAGTTGAACGAGTGAGAACGAGCAAAAACCAATGGATCAACTTCGACTACACGGC +GATTCATTGCAGCGGGTTCTCGCCCGATCCCAACTGCCGTTTAATTCTCGCATGAAGATC +CGCGTATCTGGGACTCCCACTGACGCGCTGGATAGTGAAAAGAAGTACCAGCACGCCTGC +TGTAATGGGCGGGCGGTATCCTAACCGGCCTGGAAATGGGGCACCGTTTACTCACGCGCT +GGGGTTCACAGATTTGACGTGGATTAGAATGGTTCGACCAGGTTAATCCCGGCAGCTTGA +CAAAGCACCCCGTTAAAAGTTCTTCACCAAGTCGTATCCAGCAAGAAATTCAGACTGATG +CTCTGGAAACCAAAGTTTACCGCCGTGGAATGCGAAAGAAGACAGGCAATTTATGTGACG +AATGGAAGGGCAAAAATAGCACCCGCTGGACACCGCAGCGTTACGACGCCGGTGCTGGTA +GGCGATTCGGTCGCTGTGCCGAACGGTAACAACCTCGACGCAGGGCAACGCTAGGCCGTG +GAAAGTTGCGACGACCGTTGGTTTGTCATACGACCGTTGACAAACAAGCCTGCGTACCCC +AACGCACCCGCACATCTTTTGCTATCGGCGGATTATCGTCGGTCAACGCGATGCTGGCAC +ACAAAGTGTGTTCACAGAAGGTATCACGTTGCCGCTGGAAGTATATCGCGCGGTAAGAAA +CACTTACTTCGACCGAGAAGTTATCCCGTCCATCGCCTATTACCGGAACCAGAAGTTTGC +ATGCGGTGGCGTCTGGTACTGAGAAAGAAGCGAAAAGATGAAAGGGCATCAAGCTATGAA +ACCGGCACATCCTTCCCCGTGGGCTAGCTTCTGGTCGTGCTATCGCTTCTAGGACTGCGC +CAGACGGTAGTGACCAAGCTGATTTTCGACAAATAATTCTCACCGTGGTATCGTGGTGCC +GATTGTCGGTACTAACGGCGGCGAGCTTCTGGGGTGAAATCGCCTGGCAATCGAAATGGG +GTTGTCGATGACTGAGACATCGCCTGACATCCATCGCGCACCCGACCTCATGCACGAGTC +TGTGGGGCCTAGGCGCAGGAAGTGTGTCGAAGGTAGCAATTACCACCTGCTGAACCCGAA +AAGCGAAGAAGAACGTAATTTTCGTTTGCCGGAACATCTGGCAATTAAAAAAAGCGGCTA +ACCACGCCGCCTTTTTGTAACGTCTGCCAATTTACTTTCCAATTCTTCTTGCTCCACGTT +CAGAGGAGACGTTCGCATACTGCTGACCGATGCTCGTTTTCAGCCGACAGTATGTTACTG +TTCGTTAGACGTTGTGGGCGGCTCTACCCTCTGAACTTTCTCCCGGAATAAGAAAAACCT +GTACGTTGTTCAAGGGTGCATGGCGATTGAACACGCTGGCGCGGGCGTTATCACGTTGCT +GTTGATTTCGTGGGCGCGGCTGTACTTTTTCTTAAACACCTGGGCGCTGCCCTCTGTGTG +GGACTTGAATGACGCTACGCGCTGCGTCTTCTCTCGCGGCTGGTTCTGCGGGTTTATCTG +CATTTTCTCGCGTAACCGCCTGGCGGCTGCGCTTAAGGGCGAGGCGGACTGAATGCGCTC +ACGCGCTGCCTCTCTCTCGCTGCTGGATCTTGGGTTCAGTCTGCATTCTCCGCGGAACTC +CGCGCTGCTCAGGCGAGGCGGACTGATAACGCTGACGGCGGCGTCCCTTTTTTGCCTGGG +TCAGTGGTATGGCGACGGCTGAAGCGGGAAGTCTCATAGCCCCATAGTTTCAGCTTCATT +AAACCGTACTGCCGCTGCCCCTGACGATTTGGGTATCTCGTGTAATGACTGGTGCGGCGT +GTCGTTCGTTGCTGAAACTTGATTTGCTGGCCCCTGACGCTGGCTGTCAGCGTATGGGGG +CAGGTAATTGGCGTGGCGCTCATTCTCCGTTGACACTGGTTTGTGGGAAACCGCTTTTGC +CATATCCTGATCATGACTAGGCACACATTCGGTTAGCTTTGGATTGTGCCGCCATGCCAT +ATTCTTATCAGTAAGATTGCTCCCGGGGTGATACGGTTGAAATTGTTGGACGTCGATATT +ATGTTGTCCCGTTGTTGTCTGCCAGCATTTTCCTCACGATGACCGCCAATATCGTGGTGA +TTGTAACATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGC +TGAATAGTGCGTACGTGGTAGCAACGCCCATGCTAAGCCAATCCGCGTACAAGCGTCAAC +AACGGTTCTCCGGCTGGTGGTGGCAAGATAACCGCGGATCGCAGTAATTGGCCCAGTTCC +CGTAAACCACGGGTTGGTTGTAGTTGGGAAATATAGACCACATCAGGATTGGCGGCTCAA +TGGAAATGACGTTGTTGGCCGGCTCTGTAATGCGAGGGTTGGCAGTTAAAACTGTTGGAT +CTGTATGACGGGTTGCGTACTGTCCTGTTTTACCGGTAAGCTTTCTTCGTTTGGGGTAAT +AACTTCTGTTCGTGATGACTCAGCGAGCGGTCTTGTTGCGCCAGTTTGCCGCAATCCCTG +TACCGAGTCCATCACGTCCTGCGGCTGAGCCAGAAAAGCATCGCCCAGGTTTTGCCCCAT +TGCGGGTTTTCCCCATCATGCACAATGTGGTAAAGGCCCACCAGTGATTTAACGCTGGCG +TCCCACGGCTGGGTCAGATACCGCTAATAGGCAGCATCGCCTTGTTTTGAAGTGGATTCA +CGTGCGACCATTGCACTGTTGAGTACGTTGTGGAGTAGGTTTTTGGATGCCATCACACCT +GCGAAAGTAGGGCGTCGTGGGAACGCGGACGGGCGCGACCCATTTGATCTATTTGTGCAG +TGCCGATATGCGGATTTTGACGGACGGCGGTGTTACGGGAGGTGCCGTAAACCGTTTCGC +AGGAAAGTACCCGGGCAGCGACAGATCACAGGCCAGTTGTGTCGCATTGCGGTTCC +>contig_2 +GCGAAAATCCTGGAAAGAGGCGGTGCCGCCGGTCCGTTTGCCTTCCCGGCTCCGTCGCAA +ATGTTTGAAAGCGATGGTCGGTTGGTCTGGAATTGTTCCACGGGGGCCAAACGCTGGCTA +TTTAAAGATTTCGGCGGTCCGCTTATATGGCACAAATGCTGACGCCATATTGCGGTGGCT +AAGCCAGTGACCCAATTTTCTGACCGCGACCGCCGTGACTACCGGAAGCAGGGCAGTTGG +CTCCAGCTTTCGTACGGTTAACCGAAGTGAAAAGGGTATATCCTCCATCTCACACGAAGG +CAAAAAAGTCAAGTCCCACCTGCAAAGAAAAAAACTGTTCTGTACATTGGGCGGGAATAT +TCACGATAAAACTCGTTGGCCAATCGGACCGGCGGTATCTCGTTAGCCTTGTTGCAAGGC +GCTGGTGGATAGGCAGGCGTTTGATGGATGGATGAACTGAAAGTGCGCGCTAGGGGGCTT +AAACTCGGGCTAACTGGATTAGACATACTAGCCGTTTGCCTGGGCGGCAGATTTTTGCTA +GCCTGACTTTTGGAGCTGTTTGCGCAGCCGCCGAGGGCAGGAGCGCGCTGCGCAAGCCGC +TGGGTTGTCTCCGGTGCCAAGGCGGGAAACGTTCGGCGCTTTTGACGGCGGGTTCTGCTG +GGGCGAAAGTCCACGTGGCGGTCTGCCCGGTTGAAACCTGTTTGTATTTGCTGCGACGAG +ACGGTGAACGAATACCGTGCCACGGTTTCCTGACACGAGGGCAGTGGGTCCAACCCCAAA +GGGACCTCAGGCGACGGTTATCCAAACGCGATGGACGTGGCAGGCTCGCCGAACAAACCT +GGCCGCTGCTGTGGAAAGAGTTCTTCACGCCGCCAAAAATCTGGGCAAACTGGAAAGATG +CTGGGGGTTATGCAGCCGTGGGTATGATGAAACCACTCGCAACAGACAATGGCGGTGAGG +TTAAAAGAACGGGGCTACCACCTTCGGAGCCGCTACGCGCCTGACCGTTGTAGAGCTACG +ATGCGCTGCGTTGTTCAGTTGAATCCAGGCGCAATATTGGCTTGTTCCTCGGGCACCGCC +GAACAATCCCCGGCGAAAATTTAAAGGAAGAGCGTGGAAGCGATTTCTGGGGTGAAACGT +TGGATACTGGCCAAAAGGAGCTGGGCAGAACGTGGCCTTGAATTACCCCTTGCTTTTCAA +CAATAATCTGCCCGCCGATTTTTCGCTGCGTTCGTGCGTAAATTGATTGATGAATCATCC +GAGTAGAAATCTATTGTCCACTAGTCCTTCAAATCCAGGCCGGGGCTGCTTTTATGCAGC +CCGGCTTTTTTTATTGAAGAAAATTACGGAGAAAAAATGACGAGCGGAAAGAGGAGAAAT +TCCTCCAGTAAATGCGGTAACTTAGGAGATTTTAGGAATTGCGGAGACATTAACAACGGC +CGGATCTCATCGGGTACCATCTCCCGGGTAAGCCCCGACCCTAATAGCGGGCAATGATAA +AATGGAGTAGACCTGTGAAAAAGATGTCCAATCTATCGGTTCTCGCCACCTTTCCCCTCT +GGTTCTGGTCGCCTCCCCATGGCAGTTCGCCCAGAGGCATGCGGAGATTACCGTTTAGTC +CCGTTCAGTAAAATTACAGAATTAGGGGAATCGTTGCATAATCGTGGCTTATACATGGGA +TTGGAAGGTCACTGGCGCCGACCACGGCTGCGATGGAAACAACCTTTAATGAATGGCGAG +GCAATCGCTGGCACCTACACGGACCGGCCGCCCACGGCCGCCGACCACCATATTAGATGA +AGCTCCTCAATGATTTGCATCACGGCGGTCATGGTTCCAGTGGGCAAAATCGACCGCTAA +TGTGACACATGCCGGGCAACAAATCCGGCATTCAGCCGGCCTGATGACGACGGCTGGCGC +GCCGCCCCTTATCAGGTCCTACCGTTAATTCTCTTGCAAATAAATTATTCTTGAAATCAT +CGCCATGCCTTTTCTGTAGGCGCAAGGATATAAGTGCCGTTTCGAACGCCGCTTATCCCC +GGCGATTGAGCTGCGAACTTAAACGGCCTGCTCGGTAGCGCTCTAAACACCAGAGTTTCG +CCAAGTTGCTGGAGGAATCTTTCATCCAAAGTATGTAAACACCTATCGCTATTAAAAACC +AGTCAGTGTGGCTCTGCGTTGGTCAGGCCGAATTTTGCAACATAATGAAACGGACTCACC +GAGCATCCCCGCGTGCGTTTCCTTAGGTCGGAAGCGATGGGTCTTAAAGTTGTCCGTTCT +TTCCTCATCCGGAGGCACACACGGCTGGGCTGTAGGATAAACTTACGGCATTGTCAATTT +TCTCTCGGGCTTCACCGGAATCAAAATACTCACCTGACCGCCAGATTAAATCACCCAGCT +TATGCGCCCTTGGTGCTGCGGGCGCGCCTCGTTCCAGCTTGTTGGTGATTGATGTATCTC +CCCCGGAAAGTTTTGAATGCACAGAATCTTTCCGTCGGGCATTCTCAAGGCGGATCCCCC +ATTTCCAGAACGATAAGGGTGCATCTGAAATCGTACGCGGGCGGAGTCCGGCCAATAACC +AAGCCGGAAAGCATTACGCAAAATGCTGTTGGGAGCAAAACAATCGGAAATCGTGTTCGC +TGGAGGTTTCGGCCTCGCAAGCCCGTGTTTAGACTTCAACCTTTAAACGCCAGAATCCGC +CTTTGGCGGGCCCATTCGCCGGGCGTTTGAAATCTGGCCTGCGAGTTTCATGCCATAGCG +AGCGGCGCTGGAATACCCGCCCAGTTTTGTCGCTGATGCGCATCAGACGTTGCCCTGACT +ACTTCGCGGAGGGCGTCACAGCTCTGTTCCGCCTGCCTCATTGGATCAACTGCTGGGAAT +GGGCCATACAGGCCTCCGGCAGCGTATTGAGCGCGTGGGGGTCCAAACGGGGGGGCTTTG +CGGTATTCTAAGCTGTTTTTCGCCAGGGGACAATAAGAATCAGCATAGCCCAGTCCCCTT +GCAGGAAAATTGAATCGCCGGCTTTAAGGCAAAAAAAATGAGAATGAGTTGATCGATAGT +TGGTGATTACTCCGGCGAAACTATCATCCGCACGCGTCCGGAGAGAAGCTGGCGAACCGA +TATCCGGATAACGCAATGGAATCAAACACCCGGGCTGCACGCCGAGTGTTACGCACTATG +GCGTAGAGTAATCAACTTGGCAATTGGTATGGAGCACAGGACGAGAAGCAGGTAAAGGTT +GGCGGTCCAAATTGGTAAAGTAGCCATTGCAGGCCCAGTTGATATCTGCCAGTTTGCCAC +CATCAGCGGGAAGGCTTAGCAGGGGCCGCCCGCATTGCCCGTTTGCGGAAGGTGCAAGCA +TCCGCAAACACCCAGATCGCTTTTAGGGTTTGTTCAGGCGAAAAAGAGAAGGGTATTTTG +TTTTCGGGCAATAGAATGTAGCTTGGACAACGAACTGGGAGCTGATGGCAAACAGAATAA +CCACAAGGGTAACAAACTCAGCACCCCCAGGACACCCATTAGGCACCCGCTCGCGCCTTC +TGGATAAGCTGAATACCTTCCAGCGGCATGTAGGTTGGCTGCGCGTTACCCGCCAGTAAT +CATCAGCATGGCGCTTGCCCGTACAGATGACCCAGGGTGTCGATAAAAATGCCAATCATC +TGGACAATCCCTTGCCGCTGCCGGATCGCGGGAGGCCAGGACGCCGCTGCCGCTTGCCGC +GTTTGGCGTCGAACCCATTCCCGGCCTCATTGGAAAAACATACTGCGCTGAAAAACCGTT +AGTAATCGCCCTGGCTTCAAGGTATATCCCGCCGCGCCGGCCTGCCGCTTCCTGCCAGCC +AAAAGCACTCTCAAAAAATAGACCAAATGACGTGGGGAAGTTGCCCGATATTCATTTACG +CAAATTACCAGGCTGGTCAGTACCCAGATTATCGCCACTCCAACGGGACAAAGCCCTCCA +TGGAGCCGGGCGACGCCATGAAGACCGCCGAGTGATTGCCAGCAGAGTAAAGACAGCGAG +AATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGC +GTTCGCCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAAGACGGCGAAC +AGAAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACG +GAAACTGCCCATTCGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAAC +GAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAAAAGACGGCTCCAGGTCCACCG +GCGGTAATAGCCCAGCGCAACGCCGGCCAGGTTTGCCGCTACCCACGCGCGCCGCAAGAC +TGGTACACAATGACTGAAATGAGGTTAAACCGCTGGCTTGTATGGAGTGAATGCTATTTT +TATAAGACTTTTGCCAAACTGGCGGATGTAGCGGAAACTGCACAAATTCCGGTGCGAAAA +GTGAACCAACCAACCTGCGCCGAAGAGCAGGTAAATCATTTACCGATCCCCCAAAGGACG +CGTGTTAATGGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCCGCGAT +GACTTTCCTGTGGTAAACGTTACCAAGTTGTCTTAAGAAGTTATATACGCCTACGAGGTA +CTTTGATAACTTCTGCGTAAGCCATACATGAGGGTTTTTGTATAAAAATGGCGGGCGCGA +TATCCAACGCAGTGTCAGGAAATCCGAAACAGTCTCGCCTGGCGATAACCGTCTTGTCGG +CGGTTGCGCTGGACGTTGCGTCGTGATATGCATCAGGGCAGACCGGTTACAGTCCCCCTA +CACAAGTCTGTTTAAAGAGAAATACTATCATGACGGGCAAAATTGACCTCCACTTCGTCA +AGTAACCACCGTGAGTGGGCCGACACTGGGGACATCGCGGGCAATGAAAGCTGTATCAAC +CGCGAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCGGGAATAC +CGTAAGTTGATTGATTGATGGCTGTCGCCTGGGCGAAACAGCAGGCAACGATCGCGGCGC +AGCAGATCGTGGACGCGACCGACAAACTGGCAGGTAAAATATTGGTCTGGAAATCCTGAA +ACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGC +GTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGACTGCTGGTATTAGCAACG +GATCGTATCTCTGATCAAATCTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAAGAACAG +CTGGAAAAAGAAGGCAATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCG +TGCTTGTTGCGGAAGCGGGCGTGTTCCTGATCTCTCGCCGTTTGTTGGCCGTATTCTTGA +CTGGTACAAAGCGAATAGCCGATAAGAAAGAGTACGCTCCGGCAGAAGGATCCGGGCGTG +GTTTCTGTTATCCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTTGGTT +TATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAAACTGGCAGGCTGCGACCGTC +TGACCATCGCACCGGCACTGCTAGAAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACG +TAAACTGTCTTACACCGGCGAAGTGAGAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGT +TCCTGTGCGGCAGCACAACCAGGATCCAAATTGGCAGGTCAGATAAACTGGCGGAAGGTA +TCCGTAAGTTTTGCTATTGACCAGGAAAAACTGGAAAAAAATGATCGGCGATCTGCTGTA +ATCATTCTTAGCGTGACCGGGAAGTCGGTCACGCTACCCTCTTCTGAAGCCTGTTCTGTC +ACTCCCTTCGCAGTGTATCATTCTGTGTTTAACCGAGACTGTTTTAAACGGGAAAATCTT +GATGAATACTTTACGTAATTGGCCTTAGTTTCCATCTCTGATCGCGCATCAAGCGGCGTT +TATCCAGGATAAAGGCATCCCTGCGCTGGAAGAATGGCTGACATCGGCGCTACACCACGC +CGTTTGAACTGGAAACCCGCTTAATCCCCGATGAGCAGGCGATCATCGAGCAAACGTTGT +GTGAGCTGGTGGATGAAATGAAGTTGCCATCTGGGTGCTCACCACGGGCGGAACTGGCCC +CGGCGCGTCGTGACGTAACGCCCGATGCGACGCTGGCAGTAGCGGACCGCGAGATGCCTT +GGCTTTTGGTGAACAGATGCGCCAAGATCAGCCTTGCATTTTGTACCAACTGTCGATCCT +TTCGCGTCAAGGTGGGCGGTGATTCGCAAACAGGCGCTGATCCTTAACTTAACCCGGTCA +GCCGAAGTCCTATTAAAGAGACGCTGGAAGGTGTGAAGGACGCTGAGGGTAACGTTCTGT +GTACACGGTATTTTTGCCAGCGTACCGTGACTGCATTCAGTTGCTGGAAGGGGCCATACG +GTTGAAAACGGCACCGGAAGTGGTTGCAGCCATTCAGACCGAAAGAGTGCAAGACGCGGA +CGTTAGCGAATAAAAAAATCCCCCCCGAGCGGGGGGATCTCCAAAACAATTAGTGGGATT +TCACCCAATCGGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCCATCGCC +AGATAGATTGCGCTGGCACCGCAGATCAGCCCAATCCAGCCGGCAAAGTGGATGATTGCG +GCGTTACCCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACGGTCAGGCTAAAGAA +AACGAATTGCAGAACGGCGTGCGCCTTTTCAGCGTGCCGAAGAACATAAAACAGCGTAAA +TACGCCCCCACAGACCCAGGGTAGACACCAAAGGAACTGTGCATTTGGCGCATTCGGTCA +GACCCAGTTTCGGCATCAGCAGAATCGCAACCAGCGTCAGCCAGAAAAGAAACCGTAAGA +GGTGAATGCGGTTAAACCGAAAGTGTTTGCCTTTTTTGGTACTCCAGCAGACCAGCAAAA +ATTTTGCGCGGATGCCGCCGTAGAAAAGTGCCCATGGCAAGGAATAATACCGTCCAGAGC +GAAATAACCCACGTTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGC +CCAGCGGTGCCGGATTAGCCAACACTTAGTGTTGCCCATAATTCCCTCAAAAAATCATCA +TCGAATGAATGGTGAAATTAATTTCCCTGAATAACTGTAGTGTTTTTCAGGGCGCGGCAT +AATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGGAAAATTTTTCC +CCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCA +GTACGGCTGGAATCGTCACGCGATAGGGCGCTGCCGCTGGACCGCTTTAACCCCATTTAG +TGCCGCACCTACAAGGGCCTCCCAGCCCCCGCGCCGGCGCAGCAAACCCATGCCCAAGTA +CCGCTCATTGCTGCGTGGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGA +CACACCTTTTTGCCAATAATGTTGTCTTTCATCAGCAGCGGCAGCCAGCTCTTCCAGCTC +ATTTCACCCTGGCATCGACCGCGGTGCACGAAACTCCTGCTTATGTTCCTCGTCCATTTT +CTTCCAGGTATTTACGGCAGAAATTGTTCCAGTAACTGTTGCTCAATTTCAAACGTAGAC +ATCTCTTTGTCGGCTTTTCAGCTTCAATCGCTTTGAAACATCCGAGGCAAAATGGCCCGA +TACACATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAA +TTCTCCGGCAATCAGCTGCCAGTTGCGGCGAATGTTGCTCGGGATGCCCTTCCATCGATT +TAAACAGTTCCGTTGCGCATCAGTACGCTGGAGAGGGCGAGTTTTTGCCTTTTTTCATTA +TGGGTGAGCAATCGGGGCGAAAATTTGCCAACTGTTCCTCACTACAATGCTGAAGAAAAT +CCAGATCTGAATCATTTCAGGTAATTTAACATTCATTTTTTGTGGCCTTCTATATTCTGG +CGTTAGTCGTCGGCCGAATAATTTTTCAGCGTGGCCATATCCGATGAGTTCACCGTTATG +ACCCGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGGTCGTTATCGCTGTTAATGTTGA +TCCAGTCAGTGGTTTGCCCCTTCTTTTATTTTCTGAAGGAATATTCAGGCTCTGACTGGC +GCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGC +AGAGAGTTGTATGCGTTTCACAATGCCGACAAACAGGAAGTTTTCAGCGCCAGATCGGTT +GGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGGAGTTTATGTTCGTTTTGCCTGCGCC +GTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATA +AGGATTCTCTTAGTGGGAAGGAGGTAGGGGGATGAATACCACTAGTTTACTGCTTGATAA +AGAGAAGATTCAGGCACGTAATCTTTTCTTTTTATTACAATTTTTTTGATGAATGCCCTT +GGCTGCGATTCATTCTTTATATGAATAAAATTGCTGTCAATTTTAACGTCTTGTCCTGCC +ATATCGCGAAATTTCTGCGCAAAAGGCACAGAAAATTTTTGCATCTCCCCCTTGATGACG +TTGGTTTACGACCCCATTTAGTAGGTCAACCGCAGTGAAGTGAGTCTGCAAAAAAATGAA +ATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTTACAGACTCACAACCACATGATGAC +CGAATATATAGTGGAGACGTTTAGATGGGTAAAATAATGGTATCGACCTGGGTACTACCA +ACTCTTGTGTAGCGATTTATGGATGGCAACCACTCCTCGCGTGCTGAGAGAAACGCCGAA +GGCGATCGCACCACGGCCCTTCTATCATTGCCTATACCCAGGATGGTGAAACCTCTAGTT +GGTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACACTCTGATTTGCGATTAA +ACGCCTGATTGGTCGCCGCTTCCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGC +CGTTCAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGGAAGTTAAAGGGCCAGAA +AATGGCACCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAAATGAAGAAAAACCGCTTGA +AGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTAACGAT +GCTCAGCGTCAAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTAT +CATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGGTCTGGGACAAAAGGCACTGGCAA +CCGTACTATTCGCGGTTTATGACCTGGGTGGTGTGGTACTTTCGATATTTCTATTTATCG +AAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGATTCTGGCAACCAACGGTGATACC +CCACCTGGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCA +AGAAAGATCAGGGCATTTGACCTGCGCGAACGATCCGCTGGCAATGCAGCGCCTGAAAGA +AGCGGCAGTAAAAAGCGAAAATCGAACTGTCTTCCCGCTCAGGCAGACCGACGTTAACCT +GCCATACATCACTGCAAGACGCGACCCGGTCCGAAACACATGAAACATCAAAGTGACTCG +TGCGAAACTGGAAAGCCTGGGTTGAAGATCTGGGTAAAACCGTTTCCATTGAGCCGCTGA +AAGTTGCACTGCAGGACGCTGGCCTGTCCGTATCTGATATCGACGACGTTATCCTCGTTG +GTGGTCAGACTCGTATGCCAATGGTTCAGAAGAAAAGTTGCTGAGTTCCTTGGTAAAGAG +CCGCGTAAAAGACGTTAACCCGGACGAAGCCTGTAGCAATCGGTGCTGCTGTTCAGGGGT +GGTGTTCTGACTGGTGACGTTAAAAGACGTACTGCTGCTGGACGTTACCCCGCTGTCTCT +GGGGTATCGAAACCATGGGGCGGTGTGATGACGACGCTGATCGGCGAAAAACACCACTAT +CCCGACCAAGCACAGCCAGGTGTTCTCTACCGCTGAAGACAACCAGTCTGCGGGTAACCC +ATCCATGTGCTGCAGGGGTGAACGTAAAACGTGCGGGCTGATAACCAAATCTCGCTGGGT +CAGTTCAACCCCTAGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTA +CCTTCGATATCGATGCTGGACGGTATCCTGCACGTTTCCGCGAAAGATAAAAAACAGCGG +TCAAAAGAGCAGAAAGATCACCATTCAAGGCTTTCATTCTGGTCTGAACGAAGATGAAAT +CCAGAAAATGGTACGCGACGCAGAAGTCTAACGCCGAAGCTGACCGTAAAGTTTGAAGAG +CTGGTACAGACTCGCAACCAGGGCGACCATCTGCTGCACAGCACCCGTAAGCAGGTTGAA +AGAAGCAGGCGACAAACTGGCCGGCTGACGACAAAACTGCTATCGAGTCTGCGCTGAACT +GCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAA +CTGGCACAGGTTTCCCAAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCCAGC +AGAACTGCCGGTGCTGATGCTTTCTGCAAACAACGCGAAAAGATGACGATGTTTGTCGAC +GCTGAATTTTGAAGAAGTCAAAGACAAAAAATAATCGCCCCTATAAACGGGTAATTATAC +TGACACGGGCGAAGGGGAAATTTCCTCTCCGCCCGTGCCATTCATCTAGGGGCAATTTAA +AAAAGATGGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTTCCAAAACAGCGGAAGA +GCGTGAAATCAGAAAGGCCTACAAACGCCCTGGCCATGAAATACCACCCCGGACCGTAAC +CAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTG +ACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGG +GTGGCATGGGGCGGCGGCGGGTTTTGGCGGCGGCGGCAGACTTCAGCGATATTTTTGGTG +ACGTTTTCGGCGTATATTTTTGGGCGGCGGGACGTTGGTCGTCAAACGTGCGGCGCGCGG +TGCTGATTTTACGCTATAACATGGAGCTCACCCTCGAAGAAGCTTAGTACGTGGCGGTGA +CCAAAGAGATCCGCATTCCGACTCTGGGAAGAGTGTGACGTTTGCCACGGTAGCGGTGCA +AAACCAGGTAACACAGCCGCAGACTTGTCCCGAACCTGTCATGGTTCTGGTCAGGTGCAG +ATGCGCCAGGGATTCTTCGCTGTACAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACG +CTTGAATCAAAAGATCCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAA +AACGCTGTCCGTTAAAATTCCCGGCAGGGGTGGGACACTGGAGACCGCATCCGTGCTTGC +GGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCA +GGTTAAACAGCACCCCGATTTTCCGAGCGTGGAAAGGCAACAACCTGTATTGCGAAAGTC +CCGATCAAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGG +GTCGCGTCAAACTGAAAGTTGCCTGGCGAAACCCAGACCGGTAAGCTATTCCGTATGCGC +GGTCTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCTGTGCCGCGTTG +TCGTCGAAACACCGGTAGGCCTGGAACGAAAGGCAGAAACAGGCTGCCTGCAGAGAGCTG +CAAGAAAGCTTCGTGGCGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAAGAGCTTC +CTTTGATGGTGTGTGAAGAAGTTTTTTTTGACGACCTGACCCCGCTAACCTCCCCCCAAA +AGCCTGCCCGGTGGGGCAGGCCTGGGCTAAAAATAGGGTGCGTTTGAAGATATGCGAGCA +CCTGGTAAAGTGGCGGGGAGATCACTCCCCATTAAGCGCTAACTTAAGGGTTTGTGGTAT +TACGCCTGATATGATCTTAACGGGCCGAATGAAATTAACTCTCACGATAACTGGTCCAGC +AAATTCTGGCCCCATATTGGCTAAGCCCGAAGAACTGGATACTTACGGCGCACGTAATGG +CCGGGGCTCTAACCCGCCGCCGCGAAATTCGTTGATGCTGCAACTCTGCTACGTCTGGGG +CTGGCTTACGGCCCCCGGGGGGATGTCATTAACGTGAAATCACTGCATGGGCTCAGCCTC +CATGACGTTGCAACATTTATCTTGACGTGGCTCTCCTGAAGCGGCTGCGGAATGCCCGCC +GACTGGTTTGGCATACTTGCCGCACAAACACTTGCTGTACGCGCCGCAGTTACGGGTTGT +TACAAGCGGACAAGAGATTGCGTCTTGTCGATGGGAACAGCAATTCAGTGCGCCCGGGGG +CCGGCAGCGCTGAAATGGCGACGTACATATGGGATATGATCCTTCATACCTGTCAGGTTC +ACTGGATTTTGAGCTAACCGACAGCAGAGACGCTGAAGCGGCCTGGACCGATTTGCGCAA +ACGGCAGAACGAGATACGCATTGCTGACCGGGGATTCGGTTCGCGTCCCCGAATGTATCC +GCTCACTTGCTTTGGGAGAAGCTGATTAATATCGTCCGGGTTCCACTGGCGAGGATTGGC +GCTGGTTAACTGCAGAAGGAATGCGCTTTTGACATGATGGGTTTTCTGCGCGGGCTGGAT +TGCGGTAAGAACGGTGAAACCACTGGTAATGATAGGACAATTCAGGGTAATAAAAAAAGC +CGGAGCTCCCTTTCCGGCACGTCTGCATTGCCGTATCACTTCCTCCCGAAAAAGCATTAA +TCAGTAAAACCCGACTTGCTCAGCGAGAATCGTCGAAAAGGACGAGTAGTTCAGGCGGAA +ACGCTGGAAGCAGCGGGCCATGTGCTATTGCTAACATCATTACCGGAAGATGAATATTCA +GCAGGAGCAAGTGGCTGATTGTTACCGTCTGCGATGGCAAAATTGAACCTGGCTTTTAAG +CGGCTTCAAAAGTTTGCTGCACCTGGATGCTTTGCGTGCAAAGGAACCTGAACTCGCGAA +AGCGTGGATATTTGCTAATCTACTCGCCGCATTTTTAATTGACGACATAATCCCAGCCAT +CGCTGGATTTCCCCCCCCAGAAGTGCCGGATCCGGAAAAGAAGAACTAACTCGTTGTGGA +TGAATAACAAAAAATGGTCATCTGGAGCTTACAGGTGGCCATTCGTGGGACAGTATCCCT +GACAGCCTACAAAACGCAATTGAAGAACGCGGAGGCATCGTCTTAACGAGGCACCGAGGC +GTCGCATTCTTCAGATGGTTCAACCCTTTAAGTTAGCGCTTTATGGGATCACTCCCGCGC +CGTTGCTCTTACTCGGATTCGTAAGCCGTGAAAACAGCAACCCTCCGTCTGGGCCAGTTC +GGATGTGAACCTCCACAGAGGTCTTTTCTCGTTACCAGGCGCCGCCACTACGGCGGTGAT +ACAGATGACGATCAGGGCGACAAATCATCGGCCCTTATGCTGCTTCATTGCTTCTCTTCT +CCTTGACCTTACGGTCAGTAAGACGGCACTCTACATGTGTTCAAATATAGGAGGCCCTCC +GATCTC +>contig_3 +GGCCCGGCCCGAAGATTGCGCGCCACGCCATCAATCAAGCCCCACAGGGTTAAGCGGAGA +ACTAAGGCAGCAAAACCCGTCAGCATCACACCGCGCCGAGCAGCTGCCGCCATCAACGCT +CAACGCCGCCCCAGTCGGTTGGCGTCAGCGTTCCAGTGCTCACAGAAACATGCTTTGCAA +GTTTGGTCAATATGTGAAGATAACCAGGAACGGATTGTTGCGCCGCAATAAAGGCTCACT +ACAACCCGCGAGAATCGCGTTCCCCGGCGGACAGCGTTCCCCCCAGGCGACCGCCCCAAA +GACAAATTAAGCCAACAACACAATGCCCCTGTCTGCGCAGCAAACTGGCTCGCCATCGCC +ACCAGGGACGCCCAGAGCGTAGTGACGGGTAATCCCCAGTTGCGCGCCTGTAGCACGCCA +AGGCGCGTGGCTCCGCAGCGGGTTACGCAGCACCTTGCTGAAACGCAGCCCACCAGACCC +AGACCCGCGCCCCAACCAGCAGCGAAATCGCCAGATACGCGGCAACAAGCTGTAGTGAAA +AATCATCTGCCTCGATTGACGTCAATATCCGGCGACCAGGCGCCTGCGCCCTCTGGCTAC +GCGGAGCCCTCGCGAGAAGTTCATCCAGGTGAGCGCGTAGCGGACGATTACTAACAGCGC +CAGCAATAACGCCGGGAAAAGCGCAATCGATTTACATCACCCGCTTTACCTCCCGATGGC +GTTATCCAGAACGCGCACAAAGTGCATTGCCGAGGAGCTTCGCAACCATAAAACCAGCTG +CAGGTACGCGCTGAAAGCGTCAGGCGCGGACAAACGGCAGGGCCTCGCCACAGCGGCGTT +GCCATTGAGCGCTCTTGTCTTTTGCTGTTGTCGTGATCTAATGCACGAGACTGATCACCG +TCCTTTTATACGCCGCCAGAGATCGATTACTGACGGCGGTACTGCCCCAGAAGTTGGTTT +CCCCCTTGCCAGGCCATTGGGAGCCGTACTCCAATCAAGAATTTCCTGGGAACAAGGCTC +GTTGGATCGAAGACGCAGCATATGGCGCGATCGATAAGGTCGTCAGCAATAACGGACGCG +GCACCACGCTATCACAAGCGGGGTTTCATGCTGTAGATAAAGTCTTCATATTGCCGCAAA +TGCGTTTCCGCTGCCGCTTTGCCAGGTTAAGTAAATCTGCATTTCGTAGCGATTTAGCGC +CATCGCCAACGGCTGTTTGGCCGTCAACTTGAAGTGAAATCCGCGACCCCGGCGCAATAC +CGAGTCAGCATTTCTTGGTAAAGGGCCATATCCTGCCGACCAGGACCAGTAAACCCATGG +TTTCATTTTCCGGTCAGCAGTTCAAGGTTAGGTTGCTGTGCCGCAACCGACGTTCGATCA +GCTAGTCGCATGGTGGGTCGCTGTACCCACAGGCGATAGTCGATGGGTATCCGCCACGCC +GGTAAGGCACGATGCCGAGCGCCAGCAGTAATTCCGACCGGCAACCACTCCCAGCGCCCA +CAATACGATTGGGATCAATAGCCGGCGCGCGTGTGGCGGGTATTCATTCTGCCAATAACA +ACGGAGTAAAAGCGCCCATCCGCCCGTTAACAGTCGAGCGGCGCTCGAAATAAGAAGGGT +AAGTCCCCGTCTTCATCAATGAAACTCAAAACTTCACTGGTGGCAGCCCACCCGCCGGAT +GTCGGCAAAATACCCATCTGGGATGCCCATAATCATTTCGAGGGGTTCGCCGCGCATAAC +TTCCCGCAGGCGTTCCCTGAGCACTCGTTTTCAACCGCCGGCAGGGCGACCAGATAAAAT +CACAGGCGCGTTGCCGCCACTATTTGATATACGGCACCACGGCAATGGACCGTCAGGCCA +CGGCTCCTGGACTTTAAAACGGTTGCACCCAGCGACAGCAACATCCAACCTGGTGGGCGG +ATATCCAGGCGGCCGCGAGGTCGGTCCGTCGAGCAACAGACAACGGCTATCCTGCGCCAA +CCAGCATGGCGTCCCACGCCCTGCTGACTGTCGCCGCCAGAAGAGGACTATCGCCCAGCC +GATGCGCCCAGCGGTTTTTAAAAGGCCAACCAGCGAGATAAGGCTTCCTCGACTTTGTTT +CGCGGATCTGCCGCCCGCCAAGCGCGCCCCAGCGCGGCCATGCCACCGGGTAACGACCAA +ATCGCCACCCGTTCACGCAACGGTCATCCCTTCTGCCGGGAGGAAGCTGCTGCGGCAAAA +TAAGCCACTCTGCGGGGCAGAACCGCCCTTTGCCTGCTCCACGCTTTCCAGCGGGTTAGG +GCAATCGAGAAGGAAGAATCAGCCGCCCTTCCGACGGGCGGCTGATGACGGCCAAGCATT +TTGAGCAGAAGTGGATTTAACCAGAAGCCGGTTGGGGGACCAATCAAGACCCGAGTCACT +TTCCCGGGCAGGAAAAGGTTTAACCGCACAGGCGGAATGCAATAACGCGTGCGCCCGGGG +CACACGAAAGGAGATAAATTTACGCAGTGCAAAAGTGAGTATGGGAATGATTCGTGGTAT +TCCTGCATTAACAGCCGAACTTATAGAAATGGGCAGCGGAAATCCGGTTGCCCCAGAAAG +AGAAATTAGAAACAGGGAAGGTTGCGGTTGCAACGAACCTGGACGTTTCATGCGCCCCAG +AAAACGCAGCCATAAGTAGGTTAGAAGGCAGCCTGGCGACGCTTTCACGATCGAAACGGT +TGATCTTAACATGCAGCGCCACGTTGGAGCCAGCCATGCCGCGGACTTCGCGGCCAGCAT +GACATAAACGTACTAACGCATCCACGACCGTAATAACTTCCACTCTAAAGGCAAGTTAGC +CCGGGGATCACCCATTAACTGGAATGCCCAGTATACCGACCACCGGTTGCCCAGCGTCAG +GACCTGAAGCGGACCGTCAAAGAAGGCTCGGAGTCAGCCCGACAACCCGGCAGCACATGT +GCTTTGGCACCTGTGCAGGCGTATTGCCTTTGATAGGTAGTATCGGTTGGTGGTATTCCG +CATTCGGTGTAAGTAATAGACCGAACCAGCTACGTTAACACACCTCGCCGACACGCGGGC +CGCTTTTCGCTTCGGATTTCTACCGCCTCGTGCGCGGATGTCTCGCCACCTTCAACCGAA +GAAGAAGGAACCGCTCAAGGGTCCGCCATCAGGTTGTTGAGTTGGTTTTAGTGAGATTAT +AACAACGGCCCCCAGGGTAACTTACAATCGGACGATCTTTCCGGATAGAGTATTTCAACG +CCGAACTTCATACTGCTTACCTTTTGAGACGGTGCGAAAATTTGATTACCGCGTCCTGTC +CCGCAACTTGCGAAGAAGGGTTCAAACGAGATTCGCTATAGCCTGAAGTAAGGTGTTACA +CCATCTATCAAACCAGGGTACGTTATAACACCACCAACGGCCAGGGTAAACTGTTTGTCA +TCACCGTTTATCGGGTCGTCCCGGCGAACGCGGGTAAGAGGAATTCTTGTACTTGCCCAG +TCATAAACGACCGCCTGGGTGACCAGCACATTTATTCCCAACTGCGCCATGGATCCCGAA +CACTAAACGCCCCGTTTAGTCTTGCTGGGTTATTATTCAGGCAATGCGGTAAGGGCCGGC +AGTATTGCCCGGATCTTTGGCATTGAAGGTCGAAATTCGGTTAATTCCCGGATTGACCAG +GATTGAGCAGCGGGCCACTAGATAGTCGTCGTTACCATACCAGGCGGTTGATGTGCATTA +CGCATACCGCCTAAAGTCGACAGCGGTTCAGCAGTGTGGTGTGGTCGATATTCGCCAGGT +GGCAAAACCGTTGCTCTGCAACTGGGTATCAACGGAGAAGCTTTGGGCAGCTTCTCATCC +ATCGAACCCGGACGGATTTTAACGTTGGCCTGAATAAATGGCCTTTATCCGCTGGCTGCT +AATGGCCTGCACACTGTTGGCTGTAAGCATTCGCGCGGATGCGGAGCAGACGCTCGTAGA +CCATCAAGCGCTGGTTTTTGCGGGGTTTTGTTTTTCAGCAAAGCGCAGGCTTCTGTTACG +CACAGTAATACGGTGTACGTTAAAACTCGTAGATCGAAGCTGTTAGCCGACCATCCTTCT +CATTACGAGAATAGTGTTTGGTTACTCGCCCCTTCATTAAAGATCTGTCGGCAGACGCTT +ACCAGATTCGGCCAGCGGCTCAAACGGTTCCCTCTTTCGGCAACCAGCCTGGTAATTACC +GGGTTTCTGGCCTCGGTCTGGAGGTTAAGAATAGGGAATGGTTAAAATTGGTTTTATCAT +CCGGACGACCCAGGTGAACAGCCGTGTGCTATAGCATAACGCCTGGTCTTCCTGACCCCT +TTCTGCTGGGCATTTGGCCAGCAACGCCGCAAGACCGGTCAGGCGATAAGAGTTAAACGA +CCGTCAGCATCCAACGAAATCGCCTAAAGTCAAGACCAGTCTGGAAACTGGCTGTCCAGT +ACCGGCTTTAAAACTGAACTTTTCTTTCCAGCGGTTCGGTGGTCGGACGGCTATCGGCTG +ACCCTATTCAACAGGCGCGCAGGACTGTCTTTTACCGTTAAAGCACGGAAACCCCGGGCC +ACGCAGTAATTTTCAGCGCGTTCCAGGCCATATACGGGTCAATGACCGCATCGTTTATAG +AAGTTTGCCCTGCAAACTTCAGGCCATTCAGATAGTTATCTGGCCTTGGGCTTCTGCCAC +GCAAAAGCCGCGAATGATTCGGGAGGTGTCATAGGTGTGGGATGGCCATCGCGTACTCAA +CAGATGACACCCGGCGTGTAGGACCTAAGGCGCTTTCTTTTCCCGACCTTCGGCCTGGAT +GCAGCGCCATCTCTTCGGCGGGTCCACAACAGAAATAAGGATCTGTGGCACTTTTTGGAA +TCGGCGCTATCGGGTTTTATGCCGGTAGCAGACTGTCGCGCCGCAAATAGTTGCAGCAGG +TCACCCCCCCTCGCGCTTTCTTGCGGGCGCAGGTGCACGAGCTAACGGTGATAGTTGTCT +TCGTGTCGGTTCAACCGCCTGCCCGGTGCAATTAACACAGACGTTGACCGCCTAACCCGC +CGTGGCTCCTCTACAACTGCGATTTTACGCCGTGAGTGTTTTGGCCGGGCTGAAGCATTT +GGACACCGCCGTCCATTGGGTATCATCTCTGATGGTAAAGGTGAATGATAACGTAAACGA +GAAATGATTAATTAGTAAAAGATACGAGAATATTTTCGGGTAAACGCTGGGCAGGGTTCG +CAAGCGGCTACCGGCAATTCTGAGCGTTCCTGAAGGGTTTACAATACAACCAGATGAAAA +GAAGGGTTAAAATATCTTAGATGGGATAGTTATTAGTAACCCGGGATGGCAAACTTCGCC +ATCCGGTATTTCAACGCTTAGATGTTAAGTTACTACCAAACATATCCTTGCTCCAAGCCG +GCTACAGACCGTCGCTTGTCCTTTCCGCTCTTGCTGAGCAGGTTGCTGGTTGCGGGCTTG +TTGCTGGCGGCTGAGAAAGACTGGATCAAACGGATTGCCTGACGGCCTGCTGCTGCATCT +CGGCTCTGCTGGCCACAGCGATTGCGGATCGCGGTCCAGAGCCGGCAAGATACGCGCATG +CCACCCGCTGCAAACAAAGTTGCCGTCGTAAGTCCACGCCCAATTCTGCAATATCTTCTG +CGCGGAACAAGATTCAGCGCGTTGGCGTCTGGTTAGGCCAGATAACGCTGATAAATCGAC +ATTGCCCGGCTGGCACCATACAGTTTGGTCGGCTGGTTGTTATCACGGCCGACGCAGTGA +TGGTCACCGTGCTGCCGTCATGCCCGCAAACCCAGGTATCTACGTTATTGTTGGTAGTCC +CTGTCTGTTCCTGCCAGATGCAGGTTGCGGCTATTTCGCCCCAAGGCTGACGGACCCCGT +ACCGCGTTGTACCACACTGCTGCATGGTCCCAAAGTGTCAGATACGCCGCCTGCGCCGGA +ACAGCGCGTTCCGCCTGCGGAAGCTCTGAATACAGCACACTTTGCCATCTTCCGCGATTA +CCGAACGCAGCGCAGGAAAGCGGTGCACGGTTACCACCGCTGGCGATTGGTCTGGGAATG +CCTGCGGCCACTTCGAGTTGGCGTTAAGTTCAACGCCCCCAGCAGCATTGGCCGGAACCG +GATGCAACTGATCTTTCGGTACGCCCAGTTTAATCCCAGGTCTCCGTAACCCGCAGGCAG +CCCGCAGCGCCATCCCCCAGATTTACCGTCGGGGACGTTCATCGAACGGTCAAGCGCATC +CCACCAGCATCACTCTGCCGCTTTTCGCTATAACGACGGTCATCATTCTGCGGTGACCAG +CACCTTGGCCATTCGGCTGACGCCAGCGCATTGGCGCATCCGCAATCCACGTATTCAGAA +CGATAGATTTTCGGCTGGCTTAAGGCCGTCAGATAAGTACCGCTGGTTTTACAGGGGAAC +CCAATCGAAGCGGACGCGCCTGCATCGCACGGTTGTAGCCCGCAGAACTGGCGGCTCAAG +AACCTCCGACCATCGCACGAAACTTCACCACTAAAGCGGTCGACGCACCACAATCGCAGT +TTCAAAGATCGCTCAACTTACGCTGTTTTCTTTCAGTGCCGGAATGCCTTCCACGGGCGG +CTTTTCCTGCCGCGTCCTGGGCCACCGAGTTACAAAGGTAGTGAAGATCTTCACGCCGGA +GAGATCTTTTACCTTATCGCCCAGGTTTTGCCTGGCAGCTCCTGGACGCACCAGTTGCAT +AAAGGCTGGCTGGAGGAGAGATTCACCCACCCGCGCGGCTGAACCCCCAGCGGACGGGCA +CCTCAACATGTCATAAGAGTTCTTGATCAATAATCCTGTTGCTGTTGCAGCAGAACGCAG +CACCAGATTACGTCCGCTCCAGCGGCCAGTTATTGGGTTACGCCACGCGGTTGTAGGATG +GACGGCCCTGTCACCATACCGACTAACAGCGCTGCTGAGTCGAGGCTTAGCCTCTTCTAC +CGGGCGACCAAAGTAAATACAAGCTTGCCAGCGGGAAGCCCGCGGATTTTCGTTGTCGCC +GCTCTGACCGAGATACACCCTCGTTCATATACAGCTCAAGAATACGGTCTTTGCTGTAAG +CGCGCGTCCCATGGATCAGCGCCATGGTAAGCTTCGTTCGCTTTACGCCAGGTAAGAAAC +GCTCGTCTGGAGAGGAACAGGTTTTTGCACCCAGCTGTTGCGTCAGCGTACTCGCACCCT +GTACCGTGCGGTCCGGCGGTCAGGTTTGCCAGCACCGCACGTCCGATTGAGTAGAGACTG +GATTCCATCAGCTCGTAAAAATGACGGTCTTCTTGTTCGCCCAGCAAAGTATCCACCAGC +AAAATCCGGGGAAACCACTGCGCGGCACAAACAGACGCTGCTCACCGTTTGGCGGACAGA +GGATCATGGTGACCAGACGCGGATCAAGACGGGAAGAAACCGAACCTGACGGCGTGTTCC +TCCTATTGACGATCGTCGCCAGATGATCGCCATCAAAGGTCACGACGCGCGCGCACGCTG +TCCTTCTTTACCTGTCCGGGAAAGTCAAACGGACGGCGGGAATCATCTCAATGCTGTTGG +CCTGCACGGTAAATTCGCCAGGACGGGTCATTTTCCGACACCGCTGACGATACTGGGTGC +GCTCCACAGCCAGCTTCACCATTCTCGTTCTTGCTGATGGTCATGTCCTGGCTCAAGATT +GACGATTCGGCCCGTAAACTGCCCGCAGGCAGCTTGCCAGACCTTGCCATCAATACGGCT +ACGAATTTTTTTGATCGGAGTAAAACGCCGTAAATGGCGATCAGCACGGCAAAAACGATA +GCCAGTTTTAGCAGAAGCCATAGCCAGCCGGCGTTTGCCAACGAGGCTTACGCCCCTTGC +CTTTGCCCTTACACCTTTGCGCGGCATCGGTTCCTTCATCCTCATAGTCATCATTAATCG +TCGTATAATCATCGTCATCCTTCGTAACGACGACGGCTTTACCTTTTGTTGTGACCGGAC +GGCGTCGGTTTCCCTTTGCGTCCAATTGGCTCGCGGTCATTCCCGGCCATGCTGATTTTC +TCCGCAATATTCACGGCGCAAAAGCCCGATTTTCTGTTCTTCTGTTACAAGACAGAAGAA +GATATTCTCTCAAAATTTGTGCTCGATCGGTCCCCCTCTCCCTTGAGGGAGAGGGTTAGG +GTGAGGGTGAACGTGCGCGCTGATGCCCTCACCCCCGGCCCATCTCCCCACAGGGAAGAG +GGAGAAAACCAACCTTACGAATACTTTTTCGTCCGTCGGCGTCGGTGCAGTATTTGCCGG +GGTCGTCCGGCCAGACATGTTTGGGATAACGCCCTTTTCATCTCTTTTTGCACCTCACGG +TACGCTCCTTTCCAAGAAAGTCGCTCAAATCTCGTGGTGATTTGTTAATGGGCCTTTGGG +GCAGGTGAAAGCAAACTCCAGCACCAGCGGCACGCGCCCCTGGGCGATCGTCGGATTGGT +GGGCCTTCGCCAAACATCTCTTTGCATTCTCACCCGCCAGCGCGGGCGGGTTATCCTTCA +TGATAACGAATGGCGATCCGGGCTTCCCGTCGGCACAGTGTAATGCGCAGGCAATTCACT +ATCCAGACGTTGCTGCATTCCCCCAATCAAGTAATCCGCGTAGTTGCCTGATAAATGTCG +AGTGATTTCAGCGCGCGCGTAGTGGAATGTACGCCGAGTCATATGTGTGGCAGCAGCCAC +GGTTTCCAGCCGCTGCCAATAAACTTTTCATCAATCAACCGCTGGCCAGTCATATTCCGG +CAACCACTTTGCGGCGCATAACAAACGCAAGCGTAGCTGTTCCGCTTCGCCGCGTCCAAG +TTGAGCACGCTTAAACCTTTTATCACGGATGCCCATTAAGCATCGCCTGATTGCAACTCG +TCTTCTGACGGTTTCGCCAGCGGCTGGCGACTTTCACCGTCAACTGACCGATTTGTATGC +CGGACGCCAGGCCTTTCAGCGTACCTTGCGCGTCACCCACTCCACAGTGTACAGACTGCT +GGACCAGCTTGGCGGGCAGCGTTGTACTAACTCATATAATATCGACCAGCAGCGCCATAA +AATCCCGCGCATCCGGGCGAGGCGCTGCCCTGCAATAATAACGGTGCGATCAACCATTCG +TGGGCGGCTTAAGCGCGTCGTTGGCATCGAGCATCCGCTCCCATGCCGTTTTGCCAGTTT +GATAGCGGTCCCAGTCTTGCCCACGGCGACGAGCAATGCGATCGGCAAACGCCCCGGCCA +AGTAGCGGCGGCGATAAGCGAACTGTCTGCCTCACCGCCACGTACGTTTAAGCGTTTTAA +CAGTTGCTGACTACGTTGCTGCCAGGCTGGTTGAATTGCGCGAAAACGCCACGCCCAGGT +CATTATTGCCCATCCGTGGCGGCTCGTTCGAGAATGGCGGCAACTTTTTGCCGCGGTAGC +AGCTTCGTCGTCGTTCTTCGCGCTAACCAGCATTGCCCGCTAAACGCGGGATCGTTACCC +CAGCGCTGCCATTTTTTGCCCTTGCGCACTAAGCCGTTCACCCTCCAGTGCCCCCAGCAA +TTTGTAACAGACGTTTCGCGGCCAGTAGATTCACTACTGGCGGCTTGATCAGCAGCCAAC +TCATCTGCGCCGGATCGCTGCATCCCCATTTGCAGTAATTCCATCAGCCAACCGGAAAGA +TCGCTTTGTAAGATCTCCGGTTCACTTTGCGCCCGCGGCGCGTTCTGCTTGTTTCTTTTG +GCGATTAAATGCCAGCCTGATACCCGGCTACCAGACGCCCCGGCGCGCCCGGCACGCTGC +GTCATGGATGCCCTGGCTAACGCGTTGGAGTAATCAGTCGCGTAAGCCCCGTGCGCGGAT +CAAAACGCGCCACACGCTCCTGGGGCACAATCCACCACCAGACGAAGTTACCCTTCAATG +GTTAAACTGGTTTCAGCAATATTGGTCGCCAGCACCACTTTGCGCATCCCTTGCGGTGCC +GGGAGGATCGCTTTTTCGCTGATCGTTCAGCGACAACGCGCCATACAGCGGGCAGAGCAA +TTACATCACTGCCGATGCGCGAAGCCAGTTGTTCCCTGCACACGCTGGAATTTCTCCGAC +GCCAGGGTAAAAATAACAGTAATGATCCGCTTTCCTGACGGCAGCATTTCAGCGGGTGGC +TACCGCAACGGCACTCAGTCAAAACGCTGATGCGCGGGCAGCGGTAAATAACGGCGTTCA +ACCGGAAACGAGCGCCCTTCTGAGAGAACTGACGACGGCGCCTTCTGGCCAGCATTTGCT +GCAAGCGGTCGTTGTCCAGGGTAGCCGACATAATCAGGCAGTTTAAGGTCATCACGCAGA +CCTGTTGCACACATCGAGTAACAGCGCCAACTGCCAAATCCGCCTGCAAGCTGCGCTCAT +GAAATTCATCAAGGGATCACCAGTCCAACGCCGCTCAGTTCCGGGTCACGCTGGGATCAT +GCGCCGTCAGCACGCCTTCGGTAACTCACTTCCAGGCGGGTATTCGGCCCGACGCAGTTT +TGCGCAACGCATCCGGTAGCCAACGGTATCGCCTGGCTTTTCGTTAAGCAGCTCCGCCAG +CCGTTTGCGCGAGCGTTACGCGCCGCCCAGACGACGCGGGCTCCAGCAGGGATAATTTTT +CCCGTTAATGCCGGGATTGCGCCCAGGCAGTTGCCAGCGGCAAGCCAGGTTGATTTCCCG +GCCCCGGTCGGCGCACTTAATAATACCTGCGGCGCACAATCGAGAGCGGTAAGTAATTCA +GGTAAGACGGCAGCAACGGGCAAACGACGACACAAAACGCTCCAAGAGGGTTAACATATC +TCTCGCGGCCACAATTGTAGCATCGCGGGTAATTCATAACCCGAGTGCCTCACATGTCTG +AACCGCAACGGTCTGTTTTTGCTATCGACTTACCTGCAAGAAATCCGCGAACAGATTATC +CATTTGGCGCGCCGACACACTTCCCACCCTGAGGCGGGACGTCCGGTCGCCGCCGATAAT +TTGGCATCTGACTCTGGCATTTTTAGGCGAAGTGAGCGCCGAGAAAGGAGAAGGCGCGTT +TCTCTTTAGCCGGACGGATTCGTCAACCTGGTTTTCACACTCACGCTTGATGACGCCGGA +CAATGGCTGCGTTCGCGTGTGGTGGTGGTTAGGATGCGTCAGCCGCCACGCGGCTTAATC +CAGCTGGCGAATATGCTCCGTTCACAAGGCTGAGCCCGCAGCGGTTGTTTTCAAAGCAAT +CGTCCCGTTTCATCCCACATATTACCCTTATTGCGCGACCCAGCGACGAGGCGGTGACAA +TCCCGCGCGGCCAGGTTTTAACCTGGTCGTATGCGGTGACGGGTTCACCCTTTACGCCTC +CTCGTTTGCCCGGTGGACGCACACGCTACACGCGCGCTAAAACGCTGGGCGCTAACGCAA +TAACAAGGGATTGTTCGCAATGGAATTTTTTCTCCCCCCTCTACAGCGCGCGACGCTAAT +TTCAGCGTTTACAAACGTTTTTTAGCCGATGTGATCACACCCGATGGTCGCGCAATTAAC +GCTACAGCTGCCCGAATACGGGGTGCCGATGACCGGGTTGTGCCAACCGCCTGGCCGATA +CCGCTGCTGGTATTCGACTTCAGACAACACCAAAACGGAAAATACCCACACACCTGCGGA +ATTAACTCAGAAGCCAGAGCGGCGCATTTATTTGCGTCAACAACGCTTTGGGCTAACAGG +TTGACGACAAGAGGGCTATCCTTAAATGAATCAATTTCAGAACTGTCAGGCTATAGCTCG +CTGAAAAGCGAAGTTAAAATAACGGCGCCAGAACGTCAGCCGTATTGACTTATATGTTGC +ATGGCGGATTTCGCGTCCAGACTGCTATATTGAAGGTGAAATCCGGTTACGTTAGCGGAG +AACGAACAGGGGATATTTTCCCGATGCGGTCACTGAACGAGGGTCAGAAACACCTTCGGG +AGTTGATGAGCGTAGCGGCTGAAGTGCCCAGCGTGCGGTTATCCTTTTTCGCCCGTGCTG +CATTCAGCCATTACACGGTTTTCACCCGCGCGCCACATCGATGAGAAATACCGCGCCAAC +TATTGTCAGAAGCTCAACAGAGGGGGGTAGGAAATTCTGGCTTAACAAAGCGGAAATTTC +TGCTGAAGGCATGGCTCTTTAAAAAATCACTGCCGGTTACATTGTAGTAAAGGTAAGTAG +ACTGGTTAATTTACACTCTGGTCGCGTGCGCAAGTACGCTTTTCCTCACACAGTTGTGCA +AAGTGTTACGTTTTAGTATAATTGCTATCCCGGAAAAGCATCTGCTATTTATAGCGGCCT +CATTTTTTCCCCGAACATGGGATCCGATAGTGCGTGTTAAGGAGAAGCAACATGCAAGAA +GGGCAAAACCGTAAAGACATCGTCCTGAGTATTCTCGGCCATCGCTTGGGGTGGAACCAT +ATCTAGGAGAAAGCCGGGCGAAGAGTATATGAATGAAGCCCAGCTGGCGCCACTTTCCGT +CGTATTCTGGAAGCATGGCGGTAATCAACTCAGGGGATGAAGTCGATCGCACCGGTTACA +CATATGCAGGATGAAGCAGCCCAACTTCCCGGACCCCGGTAGACCCGTGCAGCCCAGGAA +GAAGAGTTCAGCCTCGAACTGCGTAACCGCGATCGCGAGCGTAAGGCTGATCAAAAAGGA +TCGAGAAGACCGCTGAAAAAAGTGGAAGACGAAGATTTCGGCTTACTGCGAATCCTGCGG +TGTTGAAATTGGTATTCGCCGTCTGGAAGCCGCGCCCGACAGCCGATTCTGTGCATCGAC +TGCAAAACGCTGGCTGGAAATTCCGCAAAAACAGGATGGCTGGCTTAATTACAGCCGTTC +CATCACGTTTGACCACAGGCGGGAAATTGCTCCCGCCTTATTTTTTGTTCAAAGAGATGA +CAGACACACAGTATATTGGCCGCCTTCGCCCCCTCTCCTTCCCGGCGAGCTTCATTTTGG +CTCTCTGGATCGCCGCGCTCGGCAGCTATTTGCAGGCTCGCGCCCGGCAAGGTCGCCTGG +CTGGTACGCATAGAAGGATATCGACCCGCCTCGTGAAGTTCCCGGTGCCGCAGAAAACTA +TCCTGCGCCCAGCTGGAACATTACGGTGTCTGCCACTGTGGACGGCGATGTTCTCTGGCA +ATCGCAACGTCACGACGCCTATCGTGAAAGCACTCGCCTGGTTACATGAACCAAGGACCT +AAGTTATTACTGCACCTGTACGCGTGGCGCGTATTTCAACAGCATTGGCGGTATTTACGA +CGGTCATTGCCGGGTGTTGCATCATGGAACCAGACAACGCCGCAGTGCGTATTCCGCCAG +CAGCATCCCGGTCACGCAATTTGACTGACCAGCTGCGCGGCATTATTCCACAGCCGACGA +AAAACTGGCACGGGAAGATTTTAGTCATTCATGCGCCGTGATGGGGTTGTTCCGCCTACA +ACCTTGGCTGTTGTGGTTGATGATCATTTCCAGGGCGTTACAGAAATAGTGCGTTGGGGC +TGAATCTGATTGAACCAACCAGTAAGTGCAAATCTCGCTGTACCAGCTTTTTGGCTGGAA +AGTGCCAGATTACATTCATCTGCCGCTGGCGCTTAATCCACCAAGGGCGCTAAACTTTCC +CAAGCAGAAATCATGCGCCTGCGTTGCCGAAAGGCGATCCACGCCCGGTACTAATCCGCG +GCACTTCAATTTCTGGGGCAGCAGGCAGAAGCACACTGGCAGGAATTTCAGCCGTCGAAG +CAAATCCCTTCAGTCAGCCGTCAAAAACTGGGCGGCTTACCGCCGTGCCCGAGTCGGCAA +TTGTAAATTCAAACATTCTCAAATGCGTACATGCTGAGCTATGATTAGCCGCTATTTTTT +TTGTCACTGAATGATGTTTGACAACTAACCGGAGGGGTATCTATTTTTACCCCGAGTCCG +CTAATTTTTTGCCCGCAAGGTGCTAAGCCGCGAGGAAAGCGAGGCTGAACAGGCAGTCGC +CCCGTCCACCAGGGTGACGGTGATCCCGCGTGAGCAGCATGCTATTTCCCGCAAAGATAT +CAGTGAAAATGCCCTGAAAGGTAATGTACAGGCTCCAATAAAGCGGGATACCGAAGCCTG +GCTGGTTGGCGGCGGCGTGCGCGACCTGTTACTTGGGCAAAAAGCCGAAAGATTTTGACG +TAACCACTGAACGCCACGCCTGACGCAGGTGCCGCAAACTGTTCCGTAACTGCCGCCTGG +TGGGTCGCCGTTTCCCGTCTGGCTCATGTAATGTTTGGCCCCGGAGAATTATCGAAGTTG +CGACCTTCCGTTGGACACCACGAAGGTAACGTCAGCGACCGCACGACCTCCCAACGCGGG +CAAAACGGGCATGTTGGCTGCGCGACAACATTTTCGGGCTCCATCGAAGAAGACGCCCCA +GCGCCGCGATTTCACTATCATACAGCCTGTATTAACAGCGTAGCGGATTTTTACCGTCCG +TGATTACGTTGGGCGGCATGAAAGATCTGAAGGGACGGCGTTATCCGTCTGGATTGGGTA +AACCCGGGAAACGCGCTACCCGTGAAGATCCGGTACGTATGCTTGCGCGCGGTACGTTTT +TGCCGCCAAATTGGGTATGCGACATCAGCCCGGAAACCGCCAGAACCGATCCCTCGCCCT +CGCTACCCTGCTGAACGTTATCCCACCGGCACGCCTGTTTGAAGAATCGCTTAAACTGCT +ACAAGCGGGCTAACGGTTACGGAAACCTATAAGTGCTGTTGTGTGAATATCATCTGTTCC +AGCCGCTGTTCCCCGACCATTACCCGCTACTTCACGGAAAATGGCGACAGCCCGATGGAG +CGGATCATTGAACAGGTGCTGAAGAATACCCGATACGCGTATCCATAACGGATATGCGCG +TGAACCCGGCGTTCCTGTTTGCCGCCATGGTTCTGGTACCCACTGCTGGAGACGGCACAG +GAAGATCGCCCAGGAAAGGCCGGGCCTGACCTATCAACGACGCTTTGCGCTGGCGATGAA +ACGACGTGCTGGACGAAGCCTGCCGTTCACTGGCAATCCCTGAAACGTCTGACGACATTA +ACCCGCGATGATCTGGCAGTTGCCAGTTGCGGTATGTCCCGTCGTCAGGGTAAACGCGCA +TGGAAACTGCTGGAGCATCCTAAGTTCCGTGCGGCTTATGACCTGTTGGCCTTTGCCGAG +CTGAAGGTTGAGCGTAACGCTGAACTTGCAGGCGGTCTGGTGAAATGGTGGGGTGAGTTC +CAGGTTTCCCGCGCCACCAGACCCAAAAAGGGGATGCTCAACGAGCTGGGATGAAGAACC +GTCACCGCGTCCGTCGTACTTCGGTTCGTCCCACGCAAACGCGCACCACGTCGTGAGGGT +ACCGCATGACAGTGGCGGTAAATTGCCATAGGCAGCAATCTGGCCTCTCCGCTGGAGCAG +GTCAATGCTGCCCTGAAAGCATTAGGCGATATCCCTGAAAAGCCACATTCTTACCGTTTC +TTCGTTTTACCGCACCCCCACCCGCTGGGGCGCAAGAGTCAACCCGTTACTTAAACGCAG +CCGTGGCCGCTGGAAACCTCTCCTTGCACCTGAAGAGCCTACTCAAATCCACACACAGCG +TATTGAAATTGCAGCAAAGGTCGCGTCCCGCAAAGGCCTGAACGCTGGGGACCACGCACG +CTGGATCTCGACATCATGCTGTTTTGGTAATGAAAGTGATAAATTACTGAACGCCTGACC +GTTCCCGCACTACGATATGAAGAATCGTGGATTTATGCTGTGGCCGCTGTTTGAAAATCG +CGCCGGAGTTGGTTGTTTCCTGTAATGGGGAGATGTTGCGTCAAATCTTACGATACAAGA +GCATTTGACAAATTAAACAAAATGGTAATTTATTTTATATTGCGAATATAAATATTTTCT +GAAAATCATATCTCATCTCTTTCCTTAATTACGATTTTAGTTTAAACATCTCTTTGCCGG +AAATAATATAACTTCCTATTAAATAAATATCAATCCCCAATCTCCCATACCCTAACCCAA +TTGCACTTGTGAACTTATTTTTAACTTAATTTTGAATTAAGTAAATTTAATTTGTTCAAA +AGCTTCTAAAGTGCACCAATAGGCGACCTAATGAACAATGGTTGTAAATTTGGGTAACAA +AATATCATTGCTCATTAATATCCTAACATTTGTTTCTCCTATTTGTGTCCATGAATAGTC +ATTCTTCAAACATGTAGTCTGACTAATGGAATTTTATTAAAGGATTTTTATGTCTAAAAA +AATTAAGGTTTTGCCCTGTCTGGTCTTATGCTGGCGATGGTTGCAGGTACTGCTTCTGCT +GATATGGACGGCGGTCAGTTAAATATCAGTGGTCTGGTTGTTGATAACACCTGTGAAACC +CGCGGTTGACGGGCGGTAACAAGGATGGTTCTGATCCTGCTGCAAACCGCAACCGTTGGT +GAAATCGATGCTGGCGTACTGAATGACACCGTTGGCGCTAAAGCTCAAAACCTTTCAGCA +TCACCGTTGACTGCAGCAAAGCAAATCCGAATCCAGGGTAGCACGGCTAAAATGACCTTC +GGTTCTTGTTTTCTTTGGTAACAGCAAAGGGACTTTGAACAACGACATGTCTATTTAACA +ACCCCATCTGACGGCGTTAATATCGCTCTACACAATATTGATGGTATCCACTATCAAACA +GGTTCAAATTAACAACCCTGGCGATGTGTAGTACTAAAGCCCTGGATTGCAAACGACAAA +ATCTGCTGTTTATGATTTTAAAGCGTCTTACGTTCGTGCCGTCGCAGACCAAACAGCAAC +TGCTGGTTATGTAAAAAACTAACACTGCATACACCATTACTTATCAGTAAGTATTCACCA +GTTAAATTGATTGAATGAATATAACAGGGAATAAATAATTTCTATTTTATATTATTCCCC +TGTTTTAATTAAACTCTATTCAGGGATGGTTTATCGTTTTTTAACACCAAACATACAACA +AGCTTTATGCTTCGTAAACGCTGTATGGCTTTTAGTTCATCGTCTATTGCGGACATTGGT +CATTTCGGGTACTCGCGTAATAATATAAAAGCGATCAAAAAAAGTCGTCAACGTACGTCT +GGAAAATAAAGGGAATAAACCCGTTGCTTGTCCAGAGTTGGTTAGATACTGGCGATGACA +AACGCTGGAGCCTGGCAGTATTTACAGGTCCCTTTTTACTGCTACGGCCCGCCAGTATCG +CGTATTGATGCCAAACGTGGGCAAACAATCAAATTAATGGTACACAGCCAGCACCTCACT +GCCTAAAAGACAGAGAGAGCGTGTTCTGGTTTAACGTACTGGAAAGTTTCCACCAAAAAC +CAGATGCAGAAAAGGTCGCGAATCAAAGCCTGCTGCAACTTGGCATTTCGCACACGTATA +AAACTTTTCTATCCGCCCAGGATGGATTGAAGGGGAAATCCCTCTGAAGCCCCGTTAGCC +CTGAAGTGGTTCTGGTCAGGTTCAGAAGGTAAGGCGGTCATTACGAGTGACCAATCCAAC +CCCCTTACTACGTCTCTTTTAGCAGTGGTGATTTAGAAGCTAGCGGTAAACGCTATCCGA +TTGATGTGAAAAATGATTGCACCATTTAGTGATGAGGTCATGAAAAGTCAAATGGCCTTA +ATGGCAAAGCGAATTCCTGCAAAAGTGCATTTTTACGCCATTAATGACTTTGGTGGCGGC +AAATTGAAGGTAATGCCAGGCTGTAATCAGGCAAGGATATAATTCCGCAGGAAGCAATAG +CGTGACTATAGAATATACTAAAAATTATCATCATCTGACCCGTATCGCCACGTTTTTGCG +CGCTGCTGTATTGCAATACTGCTTTCAGTGCTGAACTCGTTGAATATGACCATACCTTCC +TGATGGGGCAGAATGCATCTTAATATTGATCTCAGCCGGTACAGTGAAGGTAACCCCGCT +ATACCGGGTGTTTACGACGTCAGTGTTTATGTAAACGACCAACCCAATCATTAACCCAAA +GTATTACATTTTGTCGCCAATTTAAGGAAAAAAAGAACGCCCAGGCTTGTATCACATTAA +AGAGATTTATTGCAGTTTCATATTAATTCTCCCGATATAAATAACGAAAAAGCCGTTCTG +CTTGCCAGGGGATGGAAACGCTCGGCAATTGCCTCAATTTGACGGAAGATTATCCCTCAT +GGCTTCTGTTCGTTATGACGTTAACGATCAACGTCTGGATATAGACGTTCCTCAAGCCTG +GGTAATGAAAAAATTACCAAAAACTATGTTTGATGCATCGTTATGGGAAAACGGCATTAA +TGCGGCCATGTTGTCGATACAACCTCAACCGGATATCATAGTGAAACCCCTGGTCGAAAA +AAATGAAAGCATTTATGCTGCATTTAACGGGTGGGATGAATTTTAGGTGCATGGCGACTG +CGTGCCTCCGGGCAACTACAACTGGATGACCGATTCTGGCAGTAATTATAGTTTTAAAGA +ATCGGTATGTTCAGCGTGATATCGCCTCGGCTGGCCGTTCTCAACTCATTCTTGGTGAGT +CTTATACGACGGGCCGAAACCTTTGATTTCCGTCAGTATCCGAGGCATTCGTTTATACAG +TGACAGCCGCATGTTGCCTCCGACTTTAGCCAGCTTTGCGCCTACTCATTCATGGCGTTG +CCAATACCAACGCCAAATCGACTTATTAACGCAGAGGTGGCTATAAGATTTATGAAACGA +CGGTGCCGCCAGGCGCTTTCGTCCATTGGATGATCTGAGTCCCGTCAGGGGTACGGCAGC +GATCTTATTGTTACCATCGAAAGAATCCGATGGCTCAAAGCGGTACATTCTCGCAACCTT +TCTCATCCGTTGTTCCAAATGGTTACGCCCTGGCGTTTGGGACGTTGGGATATCTAGCGG +CGGGTCAGGGTCTTAAAAGATGATATTCAGGATGAGCCTATAATTTATTTCAAGCAAGCT +ACTACTACGGCCTGAATAACTATCTGACGGGTTATACCGGTATTCAGATAACCGATAATA +ACTAATACCGCTGGTTTGTTAGGTCTTGGTCTGAATAGCTTCAGTTGGTGCATTTTTCTT +TCGATGTGACTCATTCCAATGTTCGTATCCCGGATGATAAAACATACCAGGGGCAAAGTT +TATCGTGTTTCCTGGAACAAGTTATTCGAAGAAACAAGTACTTCACTGAAATATCGCGGC +CTATCGCTATTCGACCACAGAATTACCTTGGTGCTTAATGATGCACTAAACGTCTAAATT +GATGAAAGTCGAAACATCCCGAACAAGATCCTTGGAACCGAAATCCATGCGTAATTACTC +TACCGCATGAAAAATCAGGTTACGGTCAGTATTAACCCAACCGTTGAAAGTTTTGGAGAA +AAAAGATTACGGTTCCATTTGTATCTTTTCCGGAAGTTGGTCCGATTACTGGGCTTCCGG +ACAAAATCGTGTAGCAATTACTCTATTGGCTACAGTAACAGTACATCCTGGGGGGCAGCC +TACAGTGTCAGGTGCCCAGCCGTTCATGGAACTGAAGACGGCGACACTGAACGATAGCGT +TTATCTCTAGTTTCCACCATTCCAATTGAAAAAATTACTTGGCACCTGAACAACGTACTT +CAGTGTTTTCCAGAGTATTGGATACCTCAAATAAGCAGTGACTTTAAGGGTAATAACCAA +CTCAACGTTAGCAGCAGTGGCTTATAGCGATAACGCTCGCGTCAGTTATAGCGTGAATAC +TGGCTATACGATGAATAAAGCCAGCAAAGATTGAGTTATGTTGGGGGGTTATGCCAGCTA +TGAGTCACCATGGGGGAACGCTGGCAGGTTACAATTTCTGCAAATAGCGATAACAGCCGT +CAAGTTTTCTCTCAGCACCGACGGTGGTTTTGTATTGCAATAGCGGTGGACTGGACTTTC +AGTAATGATAGTTTTAGCGACTCCGATACACTGGCGGTAGTTCAGGCTCCAGGTGCTCAA +GGAGCGCGAATAAATTATGGCAACAGTACTATCGATCGATGGGGTTATGGTGTCAACCAG +GCCGTCTTTTCTCCTTATCATGAAAACCGTATCGCGCTGGATATCAACGATCTTGAGAAC +GATGTTGGAATTAAAAAGTACCAGTGGCAGTAGCTGTACCGCGTCAGGGTTCAGTCGTCT +TTGCTGATTTTGAAACCGTGCCAAGGGCAATCAGCCATTATGAACATCACACGAAGTGAT +GGTAAAAATATTCGCCAATTTTGCTGCAGATATTATGGATGAGCAAGGCAATGTCATTGG +TAATGTTGGACAGGGTGGACAAGCATTTGTTCGTGGTATTGAAGCAGCAGGGAAATATCA +GCATTAAATGGCTCGAACAAAGTAAACCCGTAAGTTGTCTTGCGCATTATCAACAAAGCC +CAGAAGCAGAAAAAAATAGCACCAATCTATTATTCCTGAATGGAATTCGGTGGTCAGATT +TCAGTAACTACAAGGGACGTCAAATGATAAAAACAACGCCACATAAAATAGTGATACTGA +TGGGAATATTATTATACACCCTCAGTATTTTGCAACGGATATTAATGTAGAGTTTACAGC +CACTGTCAAAGCGACAACCTGTAACATCACACTTACTGGTAATAACGTCACGTAATGATG +GCAATAATAACTAACACATTGAGAATCCCTAAGATGGGTCTGGATAAGATCGCGAATAAA +ACGACAGGAATCTCAGGCTGATTTTAAACTGGTTGCCCAGTGGGTGCAGCAGTTGGCATC +AAGTTGGATTGATACCACTCTGGACCCGGAAAATGCATACATCAAGCTCACCTTAAGCTT +ATTATACCGCAGTCTGGTGATTCATCTTCGACGACAAGTAATATCGGTAATGGGTTTCAA +AAAACGGACTACTGATGATGCCACTTTCCTTAAACCTAAACAGTGCGGAAAAGATAACGC +TGGAGCACAGACGAGATGCAGCCCGATAAGGGTCTTGAGAATGACCGTTGCGCTACGTGA +AACAGATGCAGGGCAAGGGCGTACCCGGGGAATTTTCGTGCACTGGCGAACGTTTAATTT +CATCTATCAATAAAGAAGGGATAATTATAGATTGACATTTAAAAATTTTACGTTATGGAT +TATCCAGCAGTGTCGTTTTGGCTGCCTCATTGTTCAGCGTACTCTCTTATGCGGCAACAG +ACAGTATTGTGGACTGACCGTTATTACTACTGTAGAAATGGGTACTTGTACCGCTACATT +AGTAAATGACTCTGATTCAGGACATTTCCTGTTGTTGATTTTTGGTGATGTATATATTTC +TGAAATCAATTGCCAAGACCAAAGTAAAAAACATTCAAACTCAAATTCAAAGACTGTGCG +GGGTATCCCCAGATAAAAAAGCGCAAATAAAATTAACCAAGCGAGCCACATGCCGAGGGA +ACTGCTAATGACGGTGCGGGGTTTGCAAATGGTTCCACAGCCGCAGATAAAGCAAGTGCT +GTCGGCGCGTTGAAGTCTGGAGCACTTGTAACTCCCGGCAACAGGGAGTGCAACACAATT +TAGCTGTGTAACACCCAAGCATCACAAGAGGTAACAATCCTCCACTGCAGCGCCAATGCG +GTCGTTTATTATCCGATGAGTGCACCGCCTGGTCGGTGGAAAAAAATAAAACCGTAAACA +ATTGTCACTGCGGGTAAGTTTTCTGCACCAGCCACCATTTACAGTAACCTATAACTAAAC +AGACTTACAGATTCAGGAACTATATATGCATCCCACTCAACGTAAGCTGATGAAGAGAAT +AATTCTGTTTCTGTCATTACTGTTTTGCATCGCCTGTCCAGCCATTGCTGGACAGGATAT +TGATCCTTGTTGCCAATGTAAAAAACAGCACCTGCAATAAGCGGAATCAGTAAGCCAGGG +TAAATATTGATCTTGGCGGTCGTTGGGGTGGGATATTTTTCAGGTAATGGTTACTCCTGA +AAGTTATCAACCAGGGTGGAAAAGAGTTCACTTATCACTGTATCCGAACTGTGCATTACA +GGGAACTGGCGATGTGCTAAATCAGTTACATATTGATTTTAGAGCCCTTAGCGGTGTCAT +GGCTGCTGGCTCTAGGCAAATATTTGCTAAATGAAATTTTCGTCAGGAGCAAGTAATGTA +GGAGGTAGTTATGATTTTCTACTCAGGATTCGGCGAATACATTCAATGTTCTTAATGCTT +CAGGCGGATCTCGTTCCGTTTATCCAGTAATCGTCGGATGACATGAATGGGTTCATCCTG +GAAATTTAGCACCCGAATGCAAAAAATCGATCCTGCATTGAAGTGTTACATCTGGGTCAA +CTTAGTGAGCCATGTGTTAGTGGATATTTACTACGAATAACAAACAAAAACAGGCGCAAA +CCATAACAATAAAGAAACATTGGAGGTAAAAAATGAAAGACTATCTTCAGGTACATTCTT +TTTTTTAGCACTGTATTCTTGTTGTAATACAGTCAGTGCATATACAAGTTTTATTGTGGG +AAATAATGCAGGAGTTGATAACTATCGAGGCCCCTCCCACTGCCGCACAGATGACCTTTA +ATTACACATCAACAGCAAGCAACTTGGTTTTTTATAAAACCCACGCAGCTCGGGCCCGAC +TGGGGTAAAAATGTACTGGTCATACACTGGATACAGGTACCGGTGGTGTGTATTCTTATA +CTGGCAATACATCTGGCAGAGCGAATCCCTGGTTCCAAATAACGTATTGGAAATGCCATG +GTCTATTCAGGTAAAGATTATGGCGGACATTAAGACTATTTAATACATCTGTTCCTGGTC +TGTATTACACCATGTTAATATCAAGGGTCTGGTCTGCATACGATACAATAACTGACATTC +AATCGCCAGGGAATCTATATCGGAGATCCTTCCAAACCAAAGAATTTTTTCTTTTTCCGT +CACAGACAGCGATCTACAAACTAAGGGTTGCAACAAAGCAGACGACTACGATAAGTTTTG +GGCTATTGGGTGGTATAGTACACAACATAACTGTTGAATATTTTATACAGATACTAATTT +CTGATCCTACACTTAATCATGCAAGTCCAGTTATGCCAGTTCATCAAATTGACCTTTATT +CGTTTAAAGGCCTACAGTCCTGGTACAAAAGTTGTAGATCACAGCAACCACATTTATGTC +AATTTCACACTGAATAATGTCAAATTTAACCTTTACCAACATGTTTTACCTCCATACTTA +CCGGGCCATCAGTTCAATGGTTCAACGGTTAGAAATGGGAGAATAGTAGCTCTGGGACAA +TTAAAAATGGCGCTTCACCTGTTCCCTTTGATATCTCGCTTCAGAATTGTATTCGTGTGC +GTAATATTGAAAACAAAACTCGTCACTGGAAAAGTAGGTACTCAAAAACACACAACTGCT +TGGTAATACGCTTACTGGAAGCAACTGCCGCCAAAAGGCGTTGGCGTGACTCATTGAAGG +TTTAGCAACCAGTAAAAATCCGCTAATGACAGTTAAAACCTAATGATACGAATTCTGTTT +ATATAGATTATGAAACCGAAGATGCACACCTCCGATGGGGTTTACCCTAATCAAGGTAAC +GGGCACATCACAGCCTCTTCATTTCCAGGCAACATTAAAGCAAGACGGGAATATTGCTAT +CGAACCCGGCGAATTTAAAGCCACCAGTACTTTCCAGGTAACCTATCCCCTGAATAACGT +AGCATGCACTGTCCACCTTCTGGCAGTGCGCTGCTACGGATACAAATCATTGCCCCCACC +AATGTGACTGATAAAATGCCAGACAGCACAACATCAATTTATCAGGGATACGTTATGGAA +ACCGAACCACCAATCTCCTTACTGCAGAATACAAACAGGAAAAAAAACGTTTCGCGACCA +TCACCCGCTTATGACTATGAGCTTCGCCAGACTCTTTGCTGATGAAGGGCTTTAACGTCA +TGCTGGTGGGGCGATTCGCTGGGCATGACGGTTCAGGGGCACGACTCCACCCTGCCCAGT +TACCGTGCCGATATCGCCTACCACACTGCCGCCGTACGTCGCGGCCGACCAAACTGCCTG +CTGCTAGGCTGACCCTGCCGTTTATGGCGTTTGCCACGCCGGAAACAAGCCTTCGAAAAC +GCCGCAACGGGTTATGCGTGCCGGTGCTAAATGGTCAAAATTGAAGGCCGGTGAGTGGCT +GGTAGAAAACCGTACAAATGCTGACCGAACGTGCCGTTCCTGTATGTGGTCACTTAGGTT +TAACACCACAGTCAGTGAATATTTTCGTGGCTACAAAGTTCAGGGGCGCGGGCGATGAAG +CGGGCATCAACTGCTCAGGCGAATGATTAGCCTTAGAAGCTGCTGGGGCACAGCTGCTGG +TGCTGGATGCGTGCCGGTTGAACTGGCAAAACGTATTACCGAAGCGACTGGCGAGCCCCG +GTTATTGGCATTGGCGCAGGCAACGTCCACGACGGGCAGATCCTCCGCGATGATGCACGA +CGGCCTTTTGGTATTACCGGCGGTCACATTCCTAAATTCGCTAAAAAATTTCCTCGCGGA +AACGGGCGACAATCCGCGCCGGCTGTGCGGCAGTATATCGGCTGCAAGTGGTGTCCGGGC +GTTTTATCCGGGCGAAGAACACAGTTTTCCATGTAAGGAGTCAACGTTGTGTTAATTTAC +TCGAAACCGCCTGCCGCTGCTGCGTCAAGCAAATTTCGGCCGCCTGCGTATGGAAAGGCA +AGCGCGTTTTAGGCGCGGTGCCTACCAATGGTCGTAAGCCTAGCCACGCATGTGCCATAA +CGAAGCTGGTTCGACGAAGCCAAATAGGCCCCGCGCCGATGTTGGTCGTTCGCAGTATTT +TCGTTAACCCGATAGCAGTTCGACCGCCCGGAAAGATCTGGCTCGTTATCCTACGTGACC +TTTGCAGGGAGGACTGGCGAGAAGCTAAACAACGGTAAAAGTTGGATTTCGTTTTCGCCC +CTTCGGTAAAAGAGATCTACCCGAACGGTACTGAAACCCCATCACTTACGTTGGACGTTT +TCGTGGCCTTTCGACCATGCTGGAAGGTGGCCAGCCGTGCGGGACATTTTTCCGCAGGCG +TCTCGGACTATTGTCAGCCACGCTTGTTCAACCATGGTCCAGGCCGCGACATCGCGTGCT +TTCGGTGAAAAAGATTTTCAGCAAACCGGCGCTGATCCGCTAAAATGGTTGCCGATATAG +GGGCTTCGAATTATTGAGAGTGGCGGGTGGTGCCAAATTTATGCGCCGCCAAAAGACGGT +CTGGCGCTAAGTTGCCCGTAACGGTTAATCTGACGGCGGAACTAAACGCCAAATTGCGCC +TCGGTGTGTACCAAAGTTTTTAAGTTCGATGGGCTGGACCAATTGCCAGGCTGGGGGAAC +GGGATCTTCGATGAAATTATTAACCATTGCGGGGTCAAGAACTTGAATGAAAAAGGCTTC +CCGCGCCGATGCGATATTCAGATTTCGCGATGCTGCGACACATTTGCTGGAAGTTTTCTG +AAACCAGACAAACGGGCAGGTAATTCTGGTAGCCGCCTTGGCTTGGCATGATGCTCGCCT +GAGATCGACAAACAAAATGGTCGAGCTGGGGCCGTAATACTTAACTGGCGCTACGGCTGA +TGGCGCCAGTTGATTAATTTACCCGGCCACGGTCATGCTGTTCATAACACAGGTTAACTT +AAATAAAGGTTTAATTACCTTATCAATCTCAGCAAGAGGTAAACTTTGGCATCTCTGCAA +CTGTCTTTCCTCGAAGACATTCCCTTACTCAGAAGACGCTGGGCGAACTCCTGACTTACT +TCCTGTCTTCCCTGCTGAAATCCCCCTTCTCAATCCCTTTGCTCTTCAAACCACTGCGCC +AGCGTCATCATAGACTCCCCTCCCGTTTCCCATGTCTCTCAACACCCATCCGTAAAAACA +AATCCGCTTGTTCAGTATGGACCGCGTTTGCAGCATTATAGTTTTGCATGGGAACCTAAA +CGACCTTCCGCTAGTGTAGCCCTTCGTCGGATCAGCGTGACCAGTTGCCTCAAGCTAATA +ACATTAAGCTCGCGCTGGCGAATATGTTTTGTGCAGTAGTTCGAGTAATCGCAATCCGCC +GATGTTGCAATGATTTCGGTTCACTCCCGGGTGGGTGATGGTGGATATCCACCAGCGGGA +GAGGACTGTTATTAGATCGCGTCGCCGCCAGCCTCCGGCGAGTAAAACATCATCCCAAGC +CAGCCACATTTGATTAGCGGATAAGGTGGTGGCCTCGCCCCTGATAAAACAAGTTATCGG +GCACCACCGCAGCGGCAGCTTATCGGTGGGTCCAGCCTCCAGATGTCCGGTGGCATGGCG +GCTATTAGAATAACGCATCGAGGCGAAAGGACCGTTTTCTTATCCGGCCTTGCCTTTGGT +GTCCAATCCCACAACATGCAAGATAACCGGGATATTGCCCTGCATTTGCACGGAATAGAG +CCCACGTTCCCGTGCTGTGTCCTTTCAGGGCTCTCTTCAATGAAACTCGCCCCGGACTCT +AAATGAAGCGGTGTTGAGGCACAAAAGTTCGGCGTAATTCCACTGGCCAAATGTATCTCC +AGAAGTCGCGAGCCGTCTCCGCATGCATTAAAAAATTGTTTAAATAACCGCGTGATGCGG +GTTGGTACTCGGTGCATCCATCTTTCCCCTGTCTTCCGTGTGGCTTTGCGTGAAGGCAGT +ATCCGGGTCAAGGCGGCGCTATCGTCAAGCCTCCCTCTTTTATTGTTTACCGGAGCAGCA +TTCAGAGAAATATTCAATTCGTACAGGGCGGTACAATGGCACGCTTCGGCGCGGGTACGC +AAAAACTGCCCTGACCGCAAATGCCCGTCAAGATGAGATAGACACTAAAACAAAAATCGG +GCAATGACTGCGTTGAGGATTTCCGTCCAAAGCAGGCATCGGCGCTGTCGTCATACGACC +AGGGTAGTAAGGTAAGAAAGTTAGTGATTCGCTACCGATGCTGCATGGGCAAAACTTCCC +ACCCGCGGTTGAAAGGTGCTCATGCGGACACTGCACCTATGAAGGTTTCCTTTGCGCCAT +TGACCAAGGATTTTCTTGACGCCTGCCGGTATGCTCGAAAACCGAAGCCATTGATATCTT +GGAATGTCACCAACGGCAAGCGTTTCTCACACTTTATGCCTCACGCGGCAGAAGCGCCGT +TCGAGAATTTTATTTTGTTAAAACGGTGCGGCGGCCCACTGCGCGCAGTGTCGGCGAATA +TTTGTCACCATCGCCAGCTTCGTTTTACCATGCCAGATGATAGGAAGCTCGCACCTGGCG +AACCCAACGTCGCCTATTTTGGAAGTGCGATCAATGAATTGAAACCCGTACCGCGAAGTC +GATTCCCGGTACAGGTTGCTTGTATTGTCTATCCCCTGGCGGCTAGGTTAGCTCACCCAG +CCGCGACATTCGGTCTCCAGCGAATTCCGTTCTGAAGATATAGCGGTCGTTTTTACGTAA +CAACGCGAATTATCCCCCGGTTTTACTTGCCCTTTCAATGGTTGTCCACCGGGCCCAGGT +GAAATCCGGCATCGTTTGCCTGCCCTTCACGGCGGGTGTCCAATTAAATCCCGCCAAACG +GATACGAAAAGATACCAGACATGCCGGATTTAAATTTGCGCCAGAGGCGGCGGCGCGAAC +GTTGCCAAAATCAAACAGAAATATTGTGCCTCACTACGGCTCAGTAAATATGGGCGGCCG +ATAAACACATCATACCCGATGCAAAAAGATGTGTTAATGTGACTGGAAATCAAATACATT +CGGCGAATTTCGTTAAGACTCACGAAACGCTCTCATAAATTGCAAGCGAATTTTGGGTTC +CACTTCTGCGGGTGACGTCTTGATGCGTGCGGGTAACAATAGAAAAACGCCGTCCAGCCT +TCATGCCATATTGTTTCAAACACAGGATACGCATAGCGGCTCTACCGACTTGAGGCCATC +ATCAAAAGGGTAGATCCACCACCGTCTCGCGCCAGGGGAGCATTGGATCTCTTATTCTTC +ACGTAGCCTTCCCAGCTGCACCATGCCTCCAGTTTGTCGCGTAATCCCCTGTCACGCAGC +CCAGGCCATCTGGTTTATTGAGAAGCCGTACCATGTGGGTCGTCGAAGTATGGCGAAAAA +CGGAGTGTTTCTTTTCGCCGGCGCAGAATATGGTGATAGGTTAGCACCGAAGGCCATATT +CGGGTTGGGGTCTCCAGTGCGCTGATATAAGACAGCGATCGCCCATAAGGAATCTGATAC +CAGGTTTGATATAACCTGTCGTTCAGTTATGCAAATCGGGGGTAGCCGCAAATTGTTCGC +CGTACCCCCAATGGCGCATTCCCCGCACTCGGCGCGTTATTAGACCGCGTATCTTTCCAG +GTACTAAGTTCTGAATTCTCAGCGGCTTGTTGAGGTCGCCCAAACGTTCTTCAACTTTTT +TTGCGCCCCTGAACCGGCTCGAGATGACCTTTACGATTAAAAACCTTTTGCCAGAAGCCA +AAATTAAATGCGTAATAACATTGCGGCAGTGTCGCTCCACGGCAATGATTTGTGGCTCGG +CCCGATATATACCCCACGGTACCATCTTGTCACCATATGGCGCCCAGACCGCCAGCATAT +TTCGGCATGGTTTGCTATAACGGGCAAGGTAACGCGGCACTGACACTTGCGGTTAAACAG +CATCAGCAGGAGAATAACACGCTTGTTTGTCAATGAAAAAGTAACGAGGGTGAAACAAAC +CGCTTTTATTGGTAGCAAAAAGCCGCCATCAGAATCGCGGGGTTAAATACTCATGAACAA +AATTATGTTTGAGACGAAAGGCGGATTTTTTTGTTGTTGGTGCCAAAAAGACTACTCACC +TCCGGGCGTACCCAGGTTCCGCAATACGCTCGCGAACTTCTGAACTGGGTCCCATGGTTT +CACGAACAAGCATCATCTGTTCAATTAACGTAACGTGACACCAGAAAAATCACTTCGCAC +GGGGAGTGTTTGTGGCTTAAATAATCGAAGCCACCACGCGATACGGTGTGCGCTTCCGGC +TATATCGGTTAAGAAGATGGAGCACCCTACACCGGATGGTGCCGCTTACATTGCAGAAGA +GCACCGCACATCATGTCGGCTAGAGCATTAAGCAGCTTAATGCCGGCCAGAACATTCACG +GGCCGGCACTGAAGAAGTGCCCCATGTTTTTTTTTCCCAGCGCATCCAGTATCCTCTTGC +GTCCCGTCATCGTGTCAGGTAATTACCCCAACCTTAACATCGCCTTCCTCCTAGCCGAAA +ACAGGTCTTCCCTGACCATTGTTGATAAAGGGTGATAGCCGTCAAAAACTCCTATAGCCC +CCTCAAATTCCAAAGGAGAGGAAATAGCCTTAGCTACGCAAACCACGTCCACAGTGGATC +AAGCGACCAAACAAATCAAATAAAACGCCACCAATAAAAGACCCACCCAGTACGCCAAAG +GGTAGTGACCAAGCGGTAACATGATTGATTACCGAGGAGCCGTTAGCGGGAAGAACACCT +GATCATATTAAACGAATTGGGGTTCAGGTGCGAACAGGCCCTTTGCCAGAAACGGCGGGG +CAACGGAAAGTCCAGTTGGGTAAAAGACCCCCGCCCAAATACGTCGAGTGTGCGTTTTAA +CACAAAGGTTGGCACCAGGCCTGATTGTCATCGACCGTTGTGGCAAACACACCGTTCATG +CAAACCCGCAAAGGGAGAATCAACACACCGCCCGTTGAGCAGCCAGCGTTTAAGGCAACG +AAATACTCACCACGAATGCCCCTGAAATGGGCACAACAAACAAGTGCAATTGCGTCACCC +AGAAGGCCAACAAAACAGGACGACGCGTCCGCCACGCCACCGCCGACCATATCCAGGCAA +TATTGACGTGCAGTGGGGAACCGGCGCTACCAGGCAGCTCCTTCAAATATTACGCCTGTG +AACCTTGGCACCCAAAAAATGATGACGCAACGTTGGGCCGTAGGCTTTGGTGATCACCGA +CATCATGATCAGCCACCGGTACGATGAGAACTGCATATAGCTGAAGCCATGCATATCTGC +CAATACCGCGAACCAATCAGGGTTACCAGAAGATAATAAAGTAATAGGGTCATGGTGATG +ACTGGCGGCACCAGCCGTCTGCAACCCAGATACGCATCAACAGCGATGGGATCTCCTCTT +CGCCCAGCCATTGCTTTGTTAGCGCCACCCAGTAAAGCACGCATCATGACGCGACTCTCT +TGTTTTACATTAAAACCAAGTTGAAATCAAACGCTCTTCCAAGCACTGGTTAGCGTTTGT +TCACGCATCTTAATTACGCTGAATGCCCCTGCTGACTTAACCTGCGTAAATACGCTGTGT +TGTTTCCCCTGCTTCACGCAGGCACTTCAACATTCCAGCTGTCGCCGGCTATCGACCAGT +GCGAATACTGATAGGCCATCGAGCTTCGGGGTAACGGGCTTTTCGGGTGGCGAGATCGAA +GAATAAAGGCTTTTCCGATTTCCAGCTTCGCCAGTCAGGCGCCCTTCATCGAAGGTAATT +TTCCACCCAGCTTCACCCGTGTTGAATAGATGGCCGATTAATTGCGGCACAGCAGTTTCC +TGCTCCTTCCAGGGTAGTGTGTGGGTGAGAATGATGGTGGTGCCTTTTGTCGTTTTAAAA +TCCTTCAAAAGGCCCCACATTGAGCGGGCGAAGTTCAATATCCAACGCGCCGGCGGTCGG +GTCCGTCGGAGAATCAGCAGTTTAGGATTCACTCGCATTAACGCAACGGGCAATCCATTA +ACAGGGACAGCTTTCACCCGGCCAGATAACATACGCCGCACGTTCGTTGCGTTTCCCCAC +ATAGATCCGAGTATGTTCAAGATACTTTTCGCTGCGGATTGTACGCTCTCTTTGCCGCGC +CACGCCGTAGCTACCCCTGCCTGACTCACCACAATCTTGCCTGGCATGACGGTTTCAAAC +GGGTTGAAGTTAAATTCCTGCGGCCACCAGTCCCGACTGACGGTTTCCAGCGTTTCACGA +CAATCCTTCTCGAGATCGTAACCAAATACGCTGACCCGCCCGGAGGTTTTAATTTACCAG +GAGAGCTGAATAATGACCGATAGCTGGGTCGGATTTCCCGGCCCGCGTTCGGCTCCGAGA +AGCGCATAAGAAATCACCCGCTTCGACCTGGCAAATCTATCCCCACGAAGCGCCCTGAGG +ACCGGCCGCCTGGATAGGGTTTTTTTTAAGCTGTCTGAAGTTCCAGTGCAATGGTCATAA +ATTTTTTACTTACCTTTGACCGTTCTACAACTTTATCTGTGGTTTAAATCGTTCCCAGGG +AGTTGCCCTATAGTTTAGCCATAACGTAAATTATTTTGGTTTACCAGTGTCGTGTAACTC +CATGAAAGACAGAGATACACCTCACCAGCAACAAATGCCACTATGGTCAATAAATGCTGG +TGGACGAGACGATCCACGGGTTTCTTGAGAAAAGCTGGCACAAGGCGGCAAAAACCCGCG +CTTTCCTATGGATTTGGTGTTGCCGGACAAGTCGCGGTTTACCTGCATGAACGTTTAACC +GGTCTTTAGGAGCCGGGCGAACTCTTTCTGTTCACCGTAATGTTTGCTAACCTGGTCATT +CTCACTGACCGTGAACTGCCTTTCCGTGGCTTCAGTATGCGAGTGGATTGTAGCTCCGAG +GTCTGAACACATTATTATCTGTGGCCAACTGACGGTTGCGGCGGGCGTACACGGCCGGCA +GTTGAAAAAACCCGGAACTGGGGCTTATCAACAACTGGCTGCTGCAATATCACGCGTGTA +TCATGGTTCAATACTTAGCTCTCATTGCTCGGCGAAATGCCGGCAAGAGCGCCGTCTGGA +TACCTTGTTGTGAACTGAACGTGCAAATGGGAACAGGTGCATAACCTGGGCCCACGCCCA +CCACTTATGCAATCAGCGTGGATACGCGGGCAGGAAAGTTTCCCATTCACGGCTTGGGCC +ACGCGGCATTCACGACGGCTTTGCTGCGTGATCTGGATGTTTACCGGCCACCAACCGCGG +AAACCCTTGAAGCAACCGTTACCGTGCAACGGGTTATCCAACCTCAAGCCGTAAACACGC +CAACCGCAAAAAATAAAAAACATGCCCATGCCGGGATGCAACCTACTCCGGCAACTCCAC +ACCTTTACTCGTCCCAGCAGACATCAGCCTTTGCCGGATATACGGCAGATGACGGGCATA +GGCTGTGGCGTAATTTCAATGCCGTAACCCACCCACAAACCTCATCCAGGGATCGAAGAA +ACCGATAAAATTCTAACCGGGACGTTCAGCTTCACGACGGGATACCGGGTTTTATCCAGC +AGCCGTAACAAATCGCCAGCGAACTTCGGTCCGTCGCCAGCAGGCTTAGGATCTCACGCA +CCTTTCCGACAAGTGTGATTCCCCGAGTCCGGATGATATCTTCAACAATCAGCACGTCCC +TTGACTCACGGATATCTTCCGTCCAGATCTTTGAGGATTTTTCACATCACGGGTGGTGGG +ACATTGCCGGCTACCGTTAGCTGGAGGCGGTCATAAAGTCGACTTCATGGGATACCTGAA +CTTCGACGAGCACAGGCTCGGCCGATAAAACATAAACTGAGCCACCGGCAGCAAGACCCA +CCAGCTACCATATTCGCTGCCGCTTGTCATTGTAACGCTCAGTAATCTGACGACCCAGTT +CGGCGATACGCGCTTTAATCTCCGCTTAGGGGATCATTACTTCTACGTATGTTTGTCCAT +ATCTCTAACCATATGATTTAAAAACAAATCACGTTCGCACGGGCAGGTTTTGGCACCGCA +ACAAAGCGCAAGACACGCAGTATACCAGTCAAACTATTCATCCGCGGAATCTGTTAATAA +AGCTCGGAATTGTGATGACGATCACACATGGTAAAACCCTATAATTAATTGCTACTAAAA +TATTAATGAATTGAAATGGTGTCTCATTTTATGGCAATTAACAATACAGGCTCGCGACGA +TTACTCGTCACGCTAACAGCCCTTTTTGCAGCGCTTTGCGGGCTGTATCTACGTCATTGG +CGGAGGGGCTGGCTGGTCGCGATTGGCGGCTCCTGGTACTACCCTATCGCTGGCCTTGTG +ATGCTCGGCGTCGCCTGGATGCCTGTGGCGCAGTAAACGCGCCGCGCTTTGGCTATACGC +AGCCCTGCTGCTCGGCACCATGATTTGGGGCGTCTGGGAAGTTGGTTTCGACTTCTGGGG +CGCTGACTCCGCGCCAGCGACATTCTGGTCTTCTTCGGCATCTGGCTGATCCTGCCGTTT +GTCTGGCGTCGCCTGGTCATCTCCTGCCAGCGGGCGCAGTTGCCGCACTGGCGTGGTCGC +ACCTGCTGATTAGCGGTGGGTATCCTGACCTGGGCCGGATTTACCGATCCGCCAGGAGAA +TCAACAGGCACCTTAAGCGCCCGATGCCACACCTGCTGAAGCTATCTCCCCCGTAGCCGA +TCAGGGACTGGGCCTGCCTATGGGTCGTAATCAGGAAGGTCAACGCTTTTCGCCGCTGAA +ACAAATTAACGCCGATAACGTCCATAATCTGAAAGAAGCCTGGGTGTTCCGTACTGGCGG +ATGTGAAGCAGCCGAACGATCCGGGTGAAATCACCAATGAAGTGACGCCGATTAAGTGGG +CGACACCCTTTACCTGTGTACCGCCTCCACCAGCGCCTGTTTGCGCCTTGATGCCGGCCA +GCGGCCAAAGAGAAATGGCATTACGATCCTGAGCTGAAAACCAACGAGTCTTTCCAGCAC +GTAACCTGCCGTGGGTGTCTCTTAATCATGAAGCGCGCCAAAGCCAGAAACCGCTTCGCC +GAAGTGGATGGCGATTGGCCCGCGTCGTATCATTCTTCCGGTCAATGATGGTCGACTGAT +TCGCGATTAACGCTAGAAAACGGGCAAACGCTGCCGAAACCCTTCGCCAATAAAGGCGGT +GCTCAATCTGCAAAGCCAATATGCCAGACACCAAACCGGGTCTGTATGAACCGACTTCGC +CACCGATTATCACCGATAAAACCATCGTGATGGCCGGTTCAGTTACCGATAACCTTCTCA +ACCCGCGAAACGTCTGGCGTGATCCGTGGTTTTGAGATGTCAACACCGGGGAGCTGCTGT +GGGCTTTTGATCCCGGCGCGGAAAGATCACGAACGCAATCCCGTCTGACCGACACACCTT +TAACCTTTAACTCGCCAAACTCTCGGGCACCAGCGGCCTATGACGGCGAAGCTGGATTCT +GGTCTATCTGCCGATGGGCGTGACCACGCCGGATATCTGGGGGCGGTAACCGCACACCCG +GAACAGAACGTATTGCCAGCTCGATTCTGGCGCTGAATGCCACTACCGGGAACTGGCGTG +GAGGCTACCCAGACCGTTCGACCACGACCTGTGGGACATGGATCTTCCGGCACAGCCGAC +GCTGGCGGACATCACCGTTATATGGTCAGAAAGTGCCAGTTATTTACGCTCCGGCGAAAA +CCGGCAACAATTTTTTCGTGCCTCGATCGTCGTAATGGCGAACTGTGTGGTTCCGGCACC +GGAAAAAACCGGTTCCCCCAAGGTGCAGCGAAAGGCGATTCACGTAACCCCCAAACTCAA +CCGTTTTCTGAACTGAGCTTCCGTCCGACGAAAGATTTGAGCGGTAGCGGATATGTGGGG +GAGCCACCATGTTTGACCAACTGGTGGTGCCGCGTGATGTTCCCACCAGATGCGCTAGAA +GGCATTTTCACCCCGCCATCTGGAACAGGGGTACGCTGGTCCTTCCCGGGTAACCTGGGG +ATGTTCGAATGGGGCGGGATTTCCGTTGATCCAAAATCGTGAAGTGGCGATTTCCAACCC +AATGGCACTGCCCGTTTGTTTACGAAACTGATCCCGCGTGGTCCTGGCAACCCGATGGAG +CAGCCGAAAGATGCCAAAGGCCACGGGGGTACGGAATCCGGCATTCAGCCCAGTACGGTG +TACCGTATGGGTGTCACGCTCAACCCGGTTCCTCTCACCATTTGGTCTGCCATGTAAAGA +CGCCAGCATGGGGTTATATCTCGGGCGCCTGGATCTGAAAACTCATGAAGTGGTGTGGGG +AGAAACGTATTGGTTACGCCGCAAGGACAGTATGCGGTTCCCGATTGCCGGTTCCGTTTC +GCCGTTCGAATATGGGTATGACCGATGCTGGGCGGGCTCTATCTCCACGGCGGGTAACCG +TAGCTGTTTTATCGGCCAGCTACGGCCAGATAACTACCTGCGCGCTTACAACATGGGAGT +CCAACGGGTAGAAAAACTGTGGGCAGGGGTCGTTTACCCAGCGCGGGTGGTCAAGGCTAC +GGCCAATGACACTATAGAAGATGAAATGGTTAAGCAGGTATGTCGGTGATACTCCGGCAG +GCGGTCACAGGTTCATTTGGGTACCGAAAGATGGGCGGACCATATTCGTGGGTTATGCGC +TGCCGGCATGATGTGAAGATAAGACTTGCTCAGATTGCTGACAACGTGCGCGTTGTTCAT +GCCGGATGCCGGGCGTGAACGCCTTATCCAGCCTACAAATTGTGCAAAATCGATGGATTG +CACAGCTAGCGTAGGCCTGTAAGCGAGCGCATTCAGGCAGGATTTGCGTTTTGTCATCAG +TTTTAATGCCCGGAGAGAATCCGGGGCAATATTTCCGAATACGGTCTTTTTATACCCGTA +AACCCTAACATCATCCCCGTATCTTCATGCTCCAGCAGAGGCAGTGCGCCATGATAAGGC +ATGATCTTTCGGGTGCATCGTGATTAAACTTCACCAGGCACTTTCGCTGACATTACTTCT +ACCTTAACGGTATCTTTCCAGCCCGCGCGATGAGCCGCTGGCGGTTTGCCATTTTCTGAC +AAGATACGGAACTGCGTGCCGTGGATATGGAACGGATGCCAGCATCATGTCGGCCACCGC +CCAGAGATAACCCAACGTTCGTATTGCCCTTTCGCCGCCGCAAACATCGGCTTCGTTCAT +ATCCAAACGCCTGACCGTTGAATTTTGTTGGCATGGTGGAAAGTCGAACTTCCCGCCGTG +GTTCATCATGATTCATATTGCCGGTGCCCCATATGGCCCATCATCTGGCTGTGATCCATC +CCGGCCATCGCCTGATCGCCAGATTTCTCCATTTAGCATCTGCATCCCCATCAGTATCGG +AGGCATGCGGGTCCATAGAAGAGATTGCAGCTTGCGACCGTCAGCCCCCTTCCAGCGGAA +GGTAACGGCAGGCAGGCTACTTAAATGGTGTCTGGCAAAGCACCGGAGGGCACTAATAGC +AATTCGGCTGAATCCGCATTACCGGATGGAGGCTTATCAAGACGGCGCAATCGCCATCCC +CATCTGGCTGACCGGCAGCGTCACCGGTCAAAGGGTTTGTGTATCGTTAACCCTCCACCA +GCCACTTCAAAACGCTCGCCCATCAGCACCGGCAGTTCGGCTCACCTTCACTGGTTCAGG +CTAGCAGACCACCCGTCGCTGGGCAATCAATACAGCGGGCGATTGTCGCTGGTGGCGAAA +TTGAGCGAACGGGGCATTACACCCATTGAGCAAACGCAGGCGGCAGGCCAACCACGCGGG +GCAGCGTGTTGCGGGTAGTTTGCACCGTTGGTCCAGCAACGTATCGCCAAACCAGCCCAC +GGCGGCGGTCATCACATCCAGTTGATAATCAATCTGCCCGTCGGGCGCTAAAATTTCTTA +ATCCTGACGACATCACCGGAACATCATCGATACCCCACTGTTTTGGCAGCATATAAATTT +CAGGATCTCCGTTCATCTTCAATCACCACCAGCCCAGCCAGCCCCCATCGCCACCTGTCG +CCCGGTTTTGCCGTGCTGATGCGGATGGAACCCAGCAGGTAGCGGCAGGTTGATCAACGG +TTCAACGTCACCGGAAGCGGCTTGCCACCTGGCGGAATAATTCCCTGCGGGCCGCCCGCA +CTGTTCACCCGGTACTTCCAGCCCGTGCCAGTGCAACCGTTGTCTCTCTTCCGTCAGTTG +GTTGTAGATATCAACCCGTTCCGGCTTGCCGCGGCTGTAATTTCACCGCCGGCCCCAGCA +GATTTGCCGTTATAGCCCCAGGTAGTTGCAGGTTTTCCCGCCAAAGGTGGACTGGCCTGG +CGCCAATAGTTAACTCGAATGCGATTACGGGCATCGGTCGTGAGCAAATCAGGGATCGGG +TAACGATTGGGCGTTTGCCGCAAATACTGCGCGGGCTCCACAGCGGCAAAGCCCGAAGCC +ACACCCAGCGCGACGGAATATTTTAAGAAATCACGACGGTTGCATAGTTATTTCCTTATT +CTTAAGGCAGGCGAAACAAAATCAAACGTTTGAGCATAGTCCTTCCCCTTAACGGGAAGG +TCAAGCCGCAGACACAATTTAATTCGACAATAAATGGTCGTCGCCTCGCTCGCAGTGTGC +TAACGTTTATCTATCTTTACAGCCCCTGGTAGGAAGCAATGAAAGACGTTTTTCAGAACA +GTGTTATTCGGCAGCCTGATGCCGTCTGCGCAAACAGTTCGCGCTCAGGGAGTCTGAAGC +CGAAGATATGGCGCAATTTAAACGGCAGTTGTTGTCTTTCTGAAGAACGATTGTGGTTAC +CAGAACTTACCTAAACCGGGCAAATTCGTCGCGCACTGGTCTTTTTCGCTCAGCAAAACC +AGTGGGGCCTCAGTAATTACGACACCTTCGACATGAAAGCCCTCGGTGAAGACAGCTACC +GCGAATCTCAGCGGCATTGGCATTCCCGTCGCTAAAAAATGCAAAGCCCCTGGCCCGCGA +TTCCTTAAGCCTGCTTTGCCTACGTCAAAGTAATCCCTGATACCTTTTTGTAGAGAATAT +TGACCGTGCATCCGCGTCAATGTTAGCTATTATGTTGCGCCCTTTTTTTACGGGTGTTAA +CAAAGGAGGTATCAACCCATGGCCGAAAAAAAACAGTGGCATGAAAACGCTACACGACCA +GTTTGGGCAGTACTTTGCGGTAGATAACGTTCCTGTATCCATGAAAAGACCGATCACCAG +GATCTGATCATTTTTGAGAAGCGCTGCATTTGGGTCGCGTAATGGCGCTGGATGGCGTAG +TACAAACCCACCGAGCGCGACGAGTTTATCTATCATGAAGATGATGACCCATCGTTCCGC +TACTGGCCCCATGGGTCACGCGAAACATGTGCTGACTTATCGCGGCGGCGACGGTGCCAT +GCTGCGGTGAAGTAACCCGAACATAAAAACGTGAGTCCAATCACGATGGTGGAAATCGAT +GCGGGTGTCGTATCGTTCTGCCGTCAGTATCTACCCAACCATAACGCCGGTAGCTACGAC +GATCCGCGCTTTAAGCTGGTGATCCGACGATGGCGTCAATTTCGTTAATCAAACCAGCCA +GACCTTTGATGTCATTATCTCCGACTAGCACCGATCCTATCGGTCCCGGCGAAAGCCTTC +TCCAGCTTCGGCATTTTTAGAAGAAGGCCAGCAAACGTTGCCTGAATCCTGGCGGTATTC +TTCGTCGCACAAAACGGCGTCGCCTTTTTACAGCAGGAAGAAGCCATCGACAGCCATCGC +AAACTCAGCTCATTACTTTCAGCGACGTTTTGGGCTTTTATCAGGCGGCGATCCCGACCT +ATTAACGGCGGTATGCATGACTTTTTGCATGGGCGACAGATAAACGACGCCCTTACGCCA +TCTCCTCAACCGAAAGTATTCAGGCGCGTTTTCTCGCTCTGGCCTGAAATGGCCGTTATG +ACAATCCGGGCAATCCATACGGCCAGCTTTTGCCTTACCTCAGTATCTGCTAAGACGCAC +TGGCTTCACAGCCCGTCCTAAGAGAAAGATAAGAAATTGAAAAAACTGAAACTGCATGGC +TCTTAATAATCTGACCAAAAGTCTGAGTTTTTGTATTTACGATATCTGGCTACGCCAAAA +CTGCCGAAGAGCGCGACGTGTATATTGCTTATACTCGATGAGACTCTATAATGCCAACCG +TCTGACCGAAATCCTGTCCAGAAACCTGTTCCATTATCGGGGCTAAATTTCTTAACATCG +CCCGCCAGGATTACGACCACAGGGTGCCAGCGTCACTATTCTGGTGAGTGAAGAACCGGT +TGACCCGAAACTCATCGACAAAAGCAGAACACCCCGGGCCCACTGCCAGAAACGGTCGTT +GCCCATCTTTGATAAAAGTCATATTTGCGTACACTACCTTACCCGGAAAGTCATCCTGAA +GGCGGTTTATGTACCTTCCGCGCCGATATTGCGAAGTCTCTACCTGCGGCGTGATTTCTC +CCGCTGAAGGCGCATGAATTACCTGATCCACCAGCTTTGAGTCCGATATCGGTAACCCAT +TGTGTATCGCGTGCGCGGTTTTAGCCGCGAACATTAACGGTATTGAAGCACTTTATCGAC +CATGAGATTAATTCGATTCAGAAACTTTATGTCTGACGATATGAAGGCGCTGTATGACAT +GGTGGATGCGAACGTCTAATCAGGGAAAATATCTTCCATACCAAGATGTTGCTTAAAGAG +TTCGACCTTTAAGCACTACATGTTCCACACCAAACCGGACAGACTTAACCGACAGCGAGC +GCCAGGAAATTACCGCTGCGCTGTGGAAAGAAATTGCGCGAGAGTTTCATTACGGGCGCA +ATATGCCAGCTGTTTAACGGCTCTGGCGGAGCTCCCAGGCTCCGCCAGATTTATTACTTC +TGCTGCACGAAATTGCGGTAAGCCGCCACGACCTGCAGAAAATCCCCAACGCCGCATAGC +GACAGGCTTTCTTCGTCGACAGTAGGTTCATCCCCTTCTTCCATTTCATCGCCAGCGAAT +TCCAGTTGATTGGGCGCGAACCAATCACCCTCTTCACCGTCCATCCACAGGGTGTATTCA +TTGCCCTGCCCGTTGCCCAGGACCGTTCGCTACCTTTCCAGTGCGTGCGCTGCTTGTTCC +ACCTTCATCAAGCAAGGCCAGGTTTTCTTTCACCTCTTCATTAAAACCAGTGCCGGACCA +CTGTCATGCCGCCCATGGACATACGCACCTGTTACCACTCCGGTAATATCGCGCAGAAAT +TCGGTAATCCCATAGTGTGTTCCTCTGCTCCCGGCAATGCACCGAAAATCGCATTGCGCC +ATTGATGTAATTATCGCAGCCTTACCGGAGAAAAAGCAGCGGAACGGCGATGGTTTAGAA +ATAAAAAAATGCCCGGAAATCTCCATTCACCGGGCATTGTGTCGTTTATGCGCAGCGCGT +GCGGCTGACTTTTTAAACCTCAGTCTGGAAAATCACCCCATCGCTTTCTCGGTTGTACTG +AGAAAGCTGGTTGGAAGTTCAGATAACGGTAAGTATCAACGGCTGTTTTAATCTACCTGC +GCCACGTAGGGTCTGTACTCTTCCGGCGTCGGCCAGTTTGCCAATCAGCGCCGCAAACAG +CCGCCCAGTTCCGCAGAAGCCAGGAAGACATTCGCGCCAGTACCCAGACGGTTCCGGGAA +GTTTACGGGGTAGAGGTGGGAAACCACCGTTGCACCGTCCGCCACACGCGCCCTGGTTAC +CCATACACAGGGAAGCAGCCAGGGATTCTCGATACGCGGCACCACTCTTACCCGAAGACG +CTGTAGTAGCCTTCTGTCGGTCAACTGTGCGGCGTCCATACGGGTTGGCCGGTGCCACCC +ACAGGCGGGTCGGCAACTGACCTTTATGCGCATCCAGCAGTTTTACCCGCAGCACGGAAG +TGACCGATGTTTGGTCATGCAGGAACCGATAAACACTTCGTCGATCTTCTCACCCTGTAC +CAGCAGACAGCGGACGGCGCGTCATCCGGGTCCGTTCGGGAGCACACAGGATTGGCTCTT +TAAATACTCCGCCAGATCGATGTCGATCACTGCCGCGTATTCCGCATCTGCATCGGCTTC +CAGCAGCTCAGGATTCGCCCGCCATTTTTCCCATGCCCTGAAATACGACGTTCCAGGGCT +ACGACGAAATCGCCGGTAAACCTTCGCGATCATCCACCTTCAGGCAGGAACCGATGGGTA +GGAGCTTCAGGTTATTCGTTGAATCGGTTCTTGTTGGTCAAGCCCTTGATGGTACACCCA +AGGCGGCAGCAATCGCTCGGCGGGGACGCATCGGTTAGCTCAAGGGGCCCTGCTCAACTT +TCAAAGATCACGGCAGTCCTTTCAATTTCCAGGATGCGGCCCAGAGAAGATGTTTTTTCT +TGCCCTTCCTTGGTCAAGCGGTTTCAGACAGACCTTGTTTGATCGCAATACATGCGGAAT +AGGCGGTGACCAGATCCGCGCGAGGGTGATGGCGCCGGCTGCATTATTGCCCCTTTGAAG +CGCAGCCAGAACGGATTCCGGCGTATCAACGGCGGCGATTTACAGCCAGGTTGTCCGGCA +GCAAACGCCACCCAGACCAGAACCCGCCCGGGAAAGGAGATACCGATCGGGGAAACGGGG +TATGGGGAGTCACCACCGGTACCGACGGTATCCGGCAGCCAGCATACCGGTTCAGCCAGG +AGTGAATGACGCCGTCGGCCCGGACGCAGCGACACACCCGCCCACGGTTTCATAAATTGA +AGTCCGCGGCAAGCGTGTGGTGCGTGGTTACAACGGTCAACTGGCCTCGGATACGCGCGG +TGTGGCAGAAAGACTGCATCACCAGGTCAGCCCGAGAAGCCCAGGCCGCCAAGGTCTTTC +AGTTTCATCACGGGGGTCCATCGGGCCGGTGGTGTCCTGGGAAACCTACTGAAGTCATTT +TCGGTTCACAGTACGCGCCCGGACGGAAAAGTGCCTTTACAACGCCCACAGGCTCGGCCT +ACACATTTTTTTTGCGCCAGCGAGAAGCCGCGATCGCATCTCAGCGCACATCTTTCGCCT +GACGGAAGCACATCCACTTGTGCGGCAGACCAAGTGCTTCACGCCGCTTTGGTGGTCAGG +CCACGCCCGATAATCAGCGCGAAATACGGGCCCACCAGCACGCACTTCATCAATCAGCAA +CGGCGGTTTTCAGTTCGAAGGGGTTCGCCCAGCAACGTTCGCCGGTTTCGTGGTTACGAC +ACTTCACCTTTGTGATCGGGTAAACGGCAATCACGTCGCCCATGTTCAGGGTTAGAGACG +TTCGCAAACTTCGATTGGCAGTGCACCCAGCTGTCTTCCATCGTGCATTTAAGAAAGATG +GGTTGCAATTTTACCGCCGAGGCTCAAACCACCGCCGCGTTTTGTTCGCACATGTGGGAA +CATCATACGCCCCATAACCCAGAACGGAGTCTAGTGGCGGTGATTTACGCCGAAGAACCC +GGTACCCAACAACGTCACCGACGTACGCCCAGCGGGAAACATTTCTGTTGCAGAGCTTCG +GATTTGCTTGAATCGGACCAACAACACCAGGCTGGTCTGGCTCAATACCTTCACGGGGCG +TTTTTTCAGCATCGCCGCGCGTGGCAGTGGGATATCCGGGCGTGGACCAACGCATCCGGA +GCCGGAAAAGTTCATCGAGTGTTAGTTTCGCCAGTGACTTTGAAGACAGTAAACGGTTGT +TTTTCCAGCAGCGGCCGGGGCGATTGAGGACCATTCGGCACCTCCGCCCAGGACTGCATA +ACCTGCTTCGCATAATTCGTTGCCCTGCTTTTCGCTTTCTCTTCTACGTTTAGA diff --git a/workflows/genome-assembly/polish-with-long-reads/test-data/assembly_polished_by_long_reads.fasta b/workflows/genome-assembly/polish-with-long-reads/test-data/assembly_polished_by_long_reads.fasta new file mode 100644 index 000000000..d9ab0956c --- /dev/null +++ b/workflows/genome-assembly/polish-with-long-reads/test-data/assembly_polished_by_long_reads.fasta @@ -0,0 +1,6 @@ +>contig_1 LN:i:25874 RC:i:12 XC:f:0.769231 +AAGTATCGCTGAACTGTGATTACTCGTCCGGGTTTGATGAACGTGGACTTTGGCAGACGTCGCACCGTAATGTTGAGATGGGCTCTACGCAATGAGGGGTTCTGCGTTGGCGAGCGGTTGAAGACCGTGTCGGAAGAAGCTGCTGAAAATGGCTATCTCTTCTCCGGCTGCTGGAAGATGATCGACCTGTCTGGCGGCGCGGCGTGCTGGTTACATCAGGCGGGCTTCGACCTGCGTATGGATGAGTTCGATCGGTAGGTAACACCATCCGTGCACTGCTTCCGACAACGCGATCTGGGTGGGTTATCGGTACTTCTCTTGACCCGGATATGATGACAGCTGCGCGTAACCCGTTGTTGCGACAGGTATCGGCATGGACAAACGTCCTGAAATCACTCTGGTGACCAATAAGCAGGTTCAGGACCAGTGTGGGATCGCTACCAGCAAGCATGGGATGGTCCGCTGACCAGAGCAGAAGCCGTTGCTAAAGTCGTGAATGACAATGCGCCGCAAACTCGAAAAGAGCCGGATTTACTGGGATATCCCAGCATTCCTGCGTAAGCGCAAGCTGATTAAGAATTTGACTGGAATTTGGGTTTCGAGGCTCTTTGTGCTAAACTGGCCCGCCGAACGTATAGTACACTTCGGTGGATAGGTAATTTGGCGGAGATAAATACGATGATCAAAAAGGACACTAAACGTATCGTCAGCGACGGGTGGTCGGTTTACATACCCGGCAAGGAAAGTGCACCCCTGACGTTACGCCCCTGCGCTCCGGCCAACACCGGCGTCATCTATCGTCGCACCGACTTGAAATCACACCCCGGTAGATTTCCCGGCCGATGCCTAAATCTGTGCGTGAACCATGCTCTGTACGGTCTGTCACGAGCATTGATGCACGGTTTCAAACCGTAGAGCCCTCAATGACGCTCTCTCCGGGCTTGGGCATCGATAACATTGTTTCGAAGTTACATGCGCGCCGGAAATTCCCCATCATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAATTTGTTCGCATCAAAGAGATGTTGTGTCAAGATTGGCCGATATGTGGCTGAATTAGCCCGGTACAATGGTTTTCGGCTGGATTTTCACATCGTTTTTAAAACCCATCCGGCTATTGATTCCAGCAATCCAGCGCTATGCTGATGAACTTCTCGCTGATGCGTTTATGCGCCAGACTCAGCCGTGCGCGTACGTTCGGTTTCATGGCGTGATATGGAAATATCTGAGTCCCGTGGTTGTGCCCTGGCGGCAAGCTTTCGATTTGTGCATCGTTGTTGACGATTATCAGCCGTACTGACGAAGACGGCCTGCGTTTTGAAGACGAAATTTGTGCTGTCACAAAATGCTCGTGCGATCCGGTACTTGTTCATGTGTTGGTGACAATATTATTTGTGCCATTTAACCGCTATAAATCCGGTCTGTACATGAATAACAATCTAGCTGCAGGCTGCTCCTGGCGCAAACAGGAAGACCCTGGGAATGATGTGAACGCTTCCAGGACGAACTGCAGAACTAGCCGTATGGCCTTCGAAAGCGCCTTTCAGCTGTACGGGCATACACGACCATTTAAGAGCTGTCGTATAAAATTCGACTGGCAAATTCGGTGGCAGCTCTCTCCGGGCCAGGTGAACCAGTGTCGTTTTGTTTTGTGAATTTATTATAAGAGCTATTAAAAAACGGGTTGCGAACCGCTGTTTTCTTAAGCACTTTCCGCCACAACTTTCTCTCATTGTCCGCGTGCTGTGGACTGCAGGCTTTAATGATAAAGATTTGTGGCGCTAAATTACGTTTGAATATGATGGGTGGCAATAACGTGCATGAAACTCAACGCCGCTGGCGACAGTTGGTAAAACGCTACTTCTGGCCAGCATCTCTTATTAGGGATGGTTGGGCGATTTAGGTTTGCCTGCGCTCAGAAACCCGCCGGCGAACCAAACGGCCCGCAAAAAGCGACAACCCGCAACCACGAGCTTCAGCCAAAGTTAACTTTGTCAATTGGCCTTGCTGGAAGCGAACACACGCCGCCCGAATTCGAACTATTCCGTTGAAATTACTGGCATCAACATGCATTCGCCACGGTAATCCGCGATCATTTCTTTTCGCAATGGCACCCAAACACTTGCCCGTTGCTGAGAATCTTTGCTATTCAGGCGCAAAATCTTGATTCTGGATACGCTAGCTGCGCTCTGACCAGGAAGGCACGCCCGTCTGAAAAAGGGTAATCGCATTGCGATTTTATGCGGCATTTTACCCCACATGCAAAAATTCTGCATCACGCCGGGCTGGATAAGCCAGGGGCAAGGCATTCCGTTGCTGGGGCCCTCCAACGCCGTCACCTAACAAGCAACTTAGAACCTTTACTTCATTATTAACTGATAACTCCGCAAGCGCGCGGGGGGGCGTTTGCGGCATTTTATTATGCCTAATCAAAATTGTTTAACTAAAGTTTATCGGTAGTCGTAACAGATCGCAACCCTGCGCCGGATTGCGCAAAGATGGTCAACATCCATCAAGCCATGAACCGGGAATGGAAAAACTCTCCGACAAAAGAACTGAAGGGAATAACCGCAGAGTTTCGTGGCACGTCTGAAAAAAGGCGAAAGTGCTGGAAAATCTGATCCCCGGAAAGCTTTCGCCGTGGTTACGTGAGGCAAGTAAGCGCGTCTTTGGTATGGTCATTCGACGTTCAGTTACTCGGCGTATGGTTCTAAGAACGGCTGCATCGCCGAAATGCGGTACCGGTGAAGGAAAAACCACTGACCGCAACGCTGCCTGCTTACCTGAACGCCACTAACCGGTAAAGGGCGTGCACACGTAGTTACCTCAACGACTACCTGGGCGCACGTGACGCCCGAAACAACCGTCCGCTTTTGAATTCCCTTGGCCCTGACTGTCGGTATCAACTGCCGGGCATGCCAGCACCGGCAAAGCGCGATAGCTTACGCGCTGCATCACTTTACGGTACGAACAAACGATACGGCTTTGACTACCTGCCGACAACATGCCGTTCAGCCCTGAAGAACGTGTACAGCGTAAACTGCACTAATGCGCTGGTGACGAAGTGGACTCGCATTCCGAGATCGATGAAGCGCGTACACCGCTGATCCATTTCCGGCCCGGCAGAGACCAGCTTCGGAATCCGTATAAACGCGTGATAAATTATTCCGCACCCTGATCCGTCAGGAAAAAGAAGACTCTGAAACCTTCCAGGGGAAGGCCACTTCTCTCGGTGGAGAAAATTCGCCAGGTGAACTGACCAACGTGGTCTGGTGCTGGATTGAAGCAACTGCTGGTGAAAGAGGGCATCATGGATGAAGAGGGAGTCTCTGTACTGCTCCGGCCAACATCATGCTCGATGCACCCGTAACGGGCGGCGCTGCGCGCTCATGCCTTGTTTTACCCGTGACGTCGACTACATCGTAAAGAGGTGAAGTTACTCATCGTTTGACGAACACACCCGGTCCGTAACCATGCAGGGGCCGTCGTCGCTGGCTCCGATGGTCTGCACCAGGGCTTTTGTGGGAAGCGAAAGAAGGTTGTGCAAGATCCAGGAACGAAAACCAAATGCCTGGCTTCGCATCACACCTTCCAGAACTAACTTTCCGTACTGGTATGGAAAACTGGCGGGGATAGACCGGTCACTGGCTGATACCGAAGCTTCCGAATTTCGTCTCAATCTACAAGCTGGATAGCCGTCGTTGCTTCCGACCAACCCGTCCCACTGGATTTTCGTAAAGGATCTAGGCAGGACCTGGGTCGTACATGACTGGAAGCGGCAAAAACTCAGGCGAATCATTGAGAGATATCGAAAGAACGTTACTGCGAAGAGGCCAGCCGGCTGCTGGGATGGGTACTATCTCACATCGGAGAAAATCGGAGCTGGTTGGTCAAACCGGAACTTGGCCAAAGCCGGGTATGAAGCACAACGTCCTTAACGGCCAAACATTCCACGCCCCAACCGAATCAGCGGGATTGTTTTGCTCAGGCGACGGTATCCGGCTGCGGTGACTATCGCGACCATATGGCGGGGTCGATGGTACAGGATATTGCTGCTTCGGTGGGTAGCTGGCAGAGCACGAAGTTGCCGCGGTCGGAAAGTCCGAACCGCCAGAGTCAAATGTGAAAAAATTAAAAGCCGACGGGGCAGGTAACGTCACGATGTCGGTACTGGGAAGCAGGTGGCCTGCATATCATCGGTCCCGGCGTCACGAATCCCGTCGATATCGATAACCAGTTGCGCGGTCGTGCTGGGTCGTTCAGGGGGATGCGGTTCTTGCCCGTTTCTGACCTGTCGTTGGAAGATGCGCTTGGATGCGTATTTTTGCTTCCGACCGGAGTTACCGGCATGCATGCGTATAACTGGGTATGAAGGCCAGGACGAAAGCCATTGAAAACACCCGTGGGTGACCTAAAGCGACTTGCCAGACGCCCCAGCCGTCACAAGTTGAAAGCCTGTAACTTCGACATTTCGTTAAGCAACTGCTGGAAATATGATTGCACGCTGGCATAGACGATCAAAGCCGTCGCGCCCATTTACTCCCAGACGTTAACGGAGACTTGTTGGGATGTTCAGCGATGTGAGCCGAAAACCATTTAACAGCATTCGGTGAAGACTGTGGTTCAAAGCGACCAATTGATTGCCTTACATTCCTACCAACCAGGGTCCTGCGGAAAGCAAAATGTGGGGAATATTCCGGGGGCTGCAGGGAACGTCTGAAGAACCGATTGTTTTCGACCTGCGCTTATGCCAAATTGCCGAGTGGCTTGGATTAAAGAATACCAGAACTAGCGATGAAGAGACGCTCGCTGAGCGCATTCTGGCTGCAAGTCCTTCGGAAGTGGTATCAGCGTAACAGAAGAAGTGGTTGGTGCTGAGATGATGCGGTCACTTCGAGAAAGGCGTCAATTGCTGGCAAACGCTTGACTCCCTTGGAAAAAGAGCACCTGGCAGCCGGTGGCCTATCTGCGTCAGGGTTACCACCTGGCAGTTGGGCTACTGGCACCAGTTGAAGAATCCGAAAGCAGGAATCCATAGACGTTAGCAATCCCGTTGTCCATGTTTTGTCAGTCGATGCTGGAGAAGTCGTTGAAAATATGATAGTTAGCACGGTACGCCTGAGCAAAGTTCCAGGTACGATATGCCTGAGAAGCAGGTTGAGGAAGCTGGAACAACAGGCGTCGTAATCGGAAGCCGAGCGTTTAGCGCAATTGCAGCAGGCTTAGCCCATCAGGATGACGCACTCTGCTAGCCCGGCCAGCTGCACTGGCGGCGCAAACACTGGAGAGCGCAAAGTAGGAACGTTAACGGATCCATTTGCCCGTGCGGTTTGGGTAAAAAATACAAGCAGGTGCCATGGCCGCCTCGGCAAATAAAAGCTTAACTGTTGAAGTAAAAGGCGCAGGAATTCTGCGCCTTTTTTAGAGGTTTAAGACAATGAAAAGAGCTGAAATTCGGTCAGGTATTGATTTCGCAACGAAACAATGAAATCTTTATATCGCGTCGCGCAGCAGATGCCCATGGCCGAAAATAAATGGAGTTTCCCGGGGAAAATTGAAATTGGGTGAAAACGCCGGAACAGGGCGGTGGTGGCGTGAACTTCAGGAAGAAGTCGGGATGCTACCCCCCAAATTTTTGCTTATTTGAAAAACCTGGAATATGAATTCCCCGACAGGCATATAACACTGGTGGTTTGGCTGGTTCGAAACGCTGGGGAAAGGGGAGCCGTGGTGTAAAGAAGGGAACCCGGAGTGACGGTGGATGTCGCTGGTCGGTCTTAATGGCCGATGATTTTTCCGCCCAGCCAATACCGATAATTGCGAAGCTTAAACGTCTGTAGGTCAGTAGGCGTTTTCGCCGCATTCCGCATTCGCACAACGATGCCTGATCCGCACGCTGGCGCGTCTTATCAGGCCTAAAGGATTTCTAATTCATTGATAAATATTATGTTTTTTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACATTTGCACAAGACTGCCTGATAGCGACGCTATCGCGTCTTATCAGGCCTACGGTGCGGCATCACGAAAACAAATTCACTGCTTTGGTTCTTCCTCCAGTACATCGCTTTACAGGAAAGATCGCCACTTGCCTGGTTGGATTCCGTTTTTTCTTCAGCAGGCCCATATCTCCGAGGTCCGATCAGTGACAACGTTTGCAGCAAAATGGCCGAAACGGGCTGATTTTTCACCCCACACCACCGTTTTCGCGCAGGTTGGGCAATTCACCGTAATAGTTTTCTGACATTTACTCCTTAGCAACAGGCCAGTTCGAAATTCCAGACGTTCCCCGGTACCTGTCCGTTTTCAGGGTCCATGCGGCATACAAAAACGAAATGGAAAACGGGCTCTTATGTCCGGAAATTTGCGGATAAAGCTGTGAATCCAGCGACAGATTCAGGCGCAGAAGTCGGCATCCCACCTTATCCTTGATAAAAACCATTCAGGCTGGTTGTTTACGGAAGGGGGGCCGGACTGCGAATTAAATCCAGCACACGTAAGTGCCTGGGTGAGCGGTTCTAGGCTGCAATCCAGGTTTCTAACTGCTGTCGCGCTGCGCCTGGGGTAGGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCGCAACAGCCGCCTGGGATGCTCAGTCGCTACGCACCAGAAGCAATCAAACGATCTTCACGCAGAAATTTGGCCGTACGCGGCCGGAAAATTCAATACGCTCCCGCCGCTTTTAACTGCTGAATTAATGCTTTAATACGGCTCTGGTCCACGCCAGGCAACGCCACAATCCAGGTCTGGAGTTTACGTTGCTGCCGGTTCAAGGTCTTTCAACAGCTCAGTGCGGACTTCGCCGCGCTCGAAAACATCAGTAATTCACTGACATTACGGAAGAAAATGGCAGCGCGCCAGCGTGGTCAACGATGGGGTAATTTAACCGGCGAGATTGCTGGATCAAAAACTCAATGCGCAGCCATGGTACGCTTTTTTCGATTTAGTGGATGTTCAAAAAAGGACCTGGGCGGTCTGCATTTACGGTTTTTCCTGTTGAGACAAATACAGCGACGCAAGCTGCAAATAGTGTGCGGCAGGCGGCAACATCCGCAATGCATAGCATCCGGTGCGCCGTTATTATCAATGACAGGTCATCTGCCACGACAAGGCGGGCTTCGCGCGTTCCTGAGCAGCAAGGATAGTTCCGACATGCTCGCGAGTTACACATCGCGCTGCATGTGTGCGCTTAAAGTTTCGTTTACTGGGCTGACATCCGACCACAAGCACTCGAGTTCGCTTTTTTATATCTAGTGAGTTTTCTACAGAATGGCACAACCCACGTACATAGGGGGAGGTAGCTTGCTGGATCCTGGTGTTTGCGTCTTCTTGCTGAATCAGCGGATGCAGCAAGGGCGTTAAGCCAGTTTTCTCTTTCCGGGTTGGCGAAGATCCGCTCGCGCAAGGCCCGGGCGCTGCAAATGTTCCATCAGCAGCAAATCATGGTTACGCCAAAGTGTCAAAGCCAATGCATGAGGCAGGTGCCCTGGTTCAACCACCTGACCGCGCAATAATATCCGGCAATTCAATGACGTTAATCCGAGAATCACAAACGCATGGCAACGGTACTCTTGCCACTGCCAATGCCTCCCGCGTTAAGGCACTATATACTCATAATTTATTTCCCGGGAATATCATCAGTGATTATAAAACGTTAAAAATGAGTGCACCGAAAGCGAAATTGATGAAACGTTCGCTCACTATTACCGGTAAATTTATGGGATTGTAGCGTACAAAAAGACAATTTCGCAGTCTTGCGCCCGCATTGATTTAGTGCGTATGATGAGGTCACTGGAGTTGCCGCTCTTACCTTATACCAATTTAACCCCCAGGAATCCCGCACATGCGTACTGAAAGAAGATTGAAGTTAGGTTATAAAGACGTTCCATCCGCCCTAAACCGCTCCACTCTTAAAGCGTTACGATGTGAACTGAACGTCAATTCACCTTCAAACATTCAGTCAGAGCTGGTCCGCGTGCCGATTATCGGCCGCAAATATGGGACACCGTAGGCACATTTTCTATGGCCTTCTGCGCTGGTTCTTTTGAATTTTGACTGCTGTGCATAAAACTATTCTTCGAAGAGTGGCAGAGCGTTTACTCAACAATTCTTCCGCTGATGTGCTGAAACATGTGATGGTTTCTACCGGCACGTCTGATCGGATTTCGAAAAAACTAAACAGATTCCCGACCTGAACCCCGGCATAAACTCGTTTGTATTGACGTGGCGAATGGTTATTCCGAAACACTTCGTGCAGTTCGTTGCGAAAAGCGCGTGAAGCGTGGCCGACCAAAACACCATTTTGTGCTGGTAAACGTAGTGACTGGTGAAATGTGTGAGGAGCTTATCCTCTCAGGTGCCGATATCCGTTAAAGTTGGCATTGGGCCACAGGTTCTGTTTGTACAACTCGCGTCAAAAACCAGGCGTCGGTTATCCGCAACGTTCTTTGCGGAATCGAATTGCCGATGCTGCGCTACGTCTGGGCGGAATGATCGTTCAGCGAATTCGTGGCTGCACCACGCCGGGGCGTGTGGCGAAAGCCTTGGCGGGGTGCCGATTTCGTCATGCTGGGCGGCATGCTGGCGGGCCACGAAGAGGCGGCGGTCGCATCGGTTGAGGAAGAAACGGCGAGTAATTTTATTGCTGTTTCTACGTGCATGACTCCGGTCTGCGAGAAACGTCCGTTGGCGGCGTTGCGGGAATATCGCGCAGCAGTAGGTAAAACCGTAAGTGCCGCTGCCGAGGCCCCGAGTTGAAAATTAACTGCGCGAGAAATTTTGGGCGGCCTGCGTTCAGCTTGTACATACGTTGGGGCTTCACGCCTGAAAGACTGACCAAGCGCACCACGTTTATTTCGTGTGCAGGGAAAAGAAAACCGCATCTTCAACAACTGTAATCTCCCAACGCTGCGTGGAGCAACACGCCAGCGTTGATCCCATCCCACTCATTCGCATCGCCTAAATGGAAAAATTGGAAGATACATTGCCACACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGTAAGGCTGCGGGTTATCCGCCAGCGCCATTGTGTTTTCCCGATGATGATGGGCGAGGTTGTCTAACATGAGATCCAGAGAGCCGGATGCCTCTCCTGTTCTCACTAATTTGCAAAACAGAGCGGGCTAAAACTCACCCGTATTTTTTAGCGCCAGCCAGATGGGTTGACCGTTACTGATATCGTGCTGGATTTGGTGGTCAGAAGTTGCACCAGTACGGGCAGCGCATTGTTTCTCTGACGCTCTCTACGCCCTGTAAAAAAGTAATGCCTGCACTTTGTGTCAGCGCCAGAATCGTAAAGATCTGCGTGAGTTTTTTGTCCCCGCATCAGTGAACCCATAATCGGGATGCGTAACAGCAATTTCTGCCGCACTATAAGCCAGGTCGGTCGGCGCATCAGCAATTATTGGCTATCGCCAGCAGAAAGCCGAACAACACCAGCAGCCAGCTCCATTCGCCACTAGAAGTCTGCCAGCGTCATGATCCCCTGCGTTAGTGCCGGTAGTGGGGTGTTGAAGGTCTTATAGATAGCGGCAAACTCCGGCAGAACAAAAAATGCAGCATTGCCACAACCACCATGATTGCCATCGCTAAAATGATGATGGGGATAACGTAACGCTGATTTTCCCACTTTGTCGGGTCAACTGACGCTGGGGCTTTTTTGCTGACGCGCCCCAGTTCAAAGCAGCATTATCCAGCTTACCGGTCAGTTCACCCGTGCGGATCATCGCTGATAGAGCGGCGGAAATACCTCTGACCACGGTAATAATGGCATTGGGAAAAAGCAATGCCCTGTTCGAGATCGTCGCCAGCGATTGCAGCAACGCTTGCCATTGCTTACTGGGATGCTGTTCCGCCAGCAGAGCCAGCCCTTCAGAAAAGCGTTAAACCCTGCTTTGAGTAGCGTCGCCAGTTGATGAATGACTTCCGCGCTTTTATCTCCTCGCCACTCGCAGATTGATGGCGATTCGCTTCAGGCTTAGTGGGGTAACCATCTGTTGCTATTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCATCCCATTTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCAGAGTTGCTTATTCGCCATGCGGCATCCCCAGTACGCGGATTAACTCTTCAAAGGTGGTTAAGCCTTTGCTCTACGGCCAGGCAGCCGTTTTCAAAAAGCGTACGCATACCCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCAACGTCGGTATTAGCGAATAAGCTGACGAATGACCGGCGTTATGGGCAGAACTTCAAAATAAGGCCGTACGACCATAAAAACCGTGGTAGTAAGTACACAACCGGGTGCCTGCCCAGTGGGGCGCGGGATGGCCATACATTGTCTGGAATGGTGGATGGGCTCCCCTTTGCTGCCGGCGACAGATGTGGGCAAAGTTTTGCGTACCCGACGCTGGGCTATTTCCAGCGTAAAGCGCCGATGATACATCCCAGCGGGCGACCCCCATTGCTGTAAACGTACCAGCTTTCGAGGTGGAAATTAGTGTGTAGGGTAGGACAACACCAGTGACCAGTTTGCGCAGCTATAATAGCGATCTCTGCGTTTCGCCATCGCGGATCTCTCCGATCAGATGACGTCAGGATCCTGGGCAATAACGACACGCAAAACCCCTGAAAGGTGAGTCAGGCACGCGGATGGATTTGCGTCTGGTTTAGTCCGGCTATGGGGATCTCAACTGGATCTGTCGACGCTACAAATATTAATGTCAGCGGTATTCAGCTTTTGCTGGGCCTATATAAAGCGTGACCGTTTTGCCGCTGCCCGGTTAGGGCCGTTACAGCACCAGTCCCTGTGGTTGTTGCAAGGCATGAGCAAAGTCCGCCAGTTGTAACGGCTGCATTCCAAGCGTGTTGACATCCAGTGCCTGACCCCACCTGGCTGTAACAACCTTAATACCACCTTTTCACCACCCCGACATGGTAAGGTCGCAATACGAAATGAGACGGCGTTTCCCTGCCAGTTCGACAGTGAATTGCCCCCGTCCTGCGGCAAGGCGATGTTCCGCAATATCCAGGTTTCCCAGCACTTTTTAATCTGGCGTTAATGCGACTCCGGCATCCGTGAAACATCCGGTAAAGGATGCAATACCCGTCGATCGCAAGCGGATGCGTAGGCATTGTCCGCTGGTTCGATATGAATATCAAACGCGCGTTGTTCCAGCGCAGATTGTAACGTTCGAGTTAGCAACTCTGCTTTTGGGCTGATGCTTCTCCTGAACAGCTACGGGCAATGTCTGTTGTGTGCGACTGGCGTGACCTTCCATTTTGTTGGCGCGTTCCAGCAGGTGATCTCAATACGTTTGGTGGTAGCGAAATGCAATGCGTCCAGTAGGCTCATGCGAAGGTGCATCGACTACCGCAACATGAACCACCTCTTCGGTGGCATCCAGCAAGACTCCATGATACCAGACACAGGGCAGTGAGCTGTGGAATATTCAATTGCCGCTCCTTAGTTGGCGTCATCAAAGCGGAAGACCATCTTCGCAGGCTTGCTGCAATTGCGCTGTCACTTTGAACTATTGCAGTTGCGCGTCCAGCCGGTGACCCGTTTGCGTTATCCCAACCCGTGTCATGACGACGCTTAGCCCCATTGAGACTTTCTTGCCCGTCAGCGACACCACGCCCTTTGCCACACTCATGGCTGAAACATAGCGGGTGGTGGTAGGCGAGGGAATGCCATTGCTGTGCCACCGTCGCAGTATCTAATCCACCATGTTCCAGCGCGCACAACTCTACGGCGGTACGGTAAGGCACAAAGGTTTGTAGCATGTCGGGTGATGCGGCTTTGCGCAGGTAGTTTTGATAAGCGGGAATACCAATGCGCTTAAAATGGGCAATGATGCCAATAACCACCATCAGTTCGATAAAGTGTAAAACCGCGTTGCTTGTCCATCTGTTTCGCTCCTTGATTTGGTTGGCGCTACTTTGGCAAAGCCATCAGCACAGGCGAGGGCAAAAAACGAAACGGGAAAGCAGATTCCGAGGTTTTTTATTTCGTTGCAGCGAAAGACAAGAAATTTGTGAGGCGTTTTACGAAGAAAGTTGGGGAAGGGGAGATTTATCCGCCCGCGATGGAGCGGATAAATCTGTCAACTCATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTAGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCCAGTGACGTTGCCAGACACTTCCAGTAGCGCCTTGCCGTTGGTGCGTTTGACGGCTTCGCGCATCTGTTCTGTTTCGAAGTTATCCAGCATGATGATATCGGCTCCTGCTTTCAGGGCTTCATCAAGTTCTTCCACGATTCTCTACCTTCGACTTCTACTGGCGCATCCGGGTGCAGCCAGGACGCTTTTTTCGACCGCCTGGCGCACTGAGCCGGAGGCAATAATATGGTTTTCTTTGATCAGGAAGGCATCAGAAAGCCCCAGACGGTGATTCGCTCCGCCGCCGCAAAGTACCGCGTATTTCAGAGCTGAACGCAGGCCGGGTAAGGTTTTGCGCGTATCCAACAACTGCGTGTTGGTGCCTTCCAGCAATTCGACATAGTGGCGTACCTTACTGGCAACTCCTGAAAGGGTTTGCACAAATTAAGCGCAGTGCGTTCGCCCGTTAACAGCACGCGGGATGGGCCTTCAAGTTCGAACAAGGATTGATTGGCATTGATGACATCGCCGTCATCCACATGCCAGATTATGGTGACATCGTCGCCTGCCAGTTGAATAAACACCTCTTCAACCCAGCGTTTGCCGCAAAAGACGCCATTCCTCCGCGGGTGATCACCGTGGCATGAGAGCGAGAATTTTTCCGGTAAAAAAGTTTTGCCGTAAAATCATTGTTGGCATCGACTGTTCCGCCTAAATCTTCCCGCAGCGCCTGGGCCACCGCGCCGGGGATATCGAGATTAATGCGTTCCAGCAGCTCGTCACGTCGGGTGTCAGGGTTATAGCGGCGAGGCGGCATGTTAAAACTCCAGATAGCTTAACGAATCATAAGGTAGAAACATGCTACTCTGAACCGGGTATTAGCACCACATATAAGGAGATCCTGCATGTTGTTAGAACAGGGGGTGGCTGGTTGGCGCGCGCCGCGTTCCCTCACCACATTACGATTGCCGCCCGGATGACGAAACACCCACCCCTGCTGGTGGTGCACAATATTAGCCTGCCGCCAGGCCGAGTTTGGCGGTCCGTGGATCGACGCATTATTCACTGGAACTATTGATCCCGCAGCACATCCTTTCTTTTGCGAGATCGCCCATTGCGCGTCTCCGTCACTGTTTGATTCGCCGTGATGGTGAAATATCCAGTATGTTCCTTTCGATAAACGTGCATTGGCATGCGGGAGTCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTTCTATTGGGATTGAGCTTGAAGGCCCGATACGCTGGCGTATACCGATGCGCAGTAATCAACAGCTTGCGGCGGTTACGCGGCACTGATTGATTGCTATCCGGATTATCGCTAAAAACATGCGGGGCATTGTGATATTGCGCCGGATCGGAAAACCGATCCCGGTCCCTGCATTTTTTGGGCACGGTTTTCGTGTGCTTGGTCAGCAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATACGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTCGGGCGCACGTTAGGCATGACCATTATTGCGATGGCGTGACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCCGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCACCATGATTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTATTTGCGTGAGCTGCAAAATGCATTGCTGTGGATTAACTTTCGTTTTTATCTTGCACCGCTGTTCTGGCTGATTGTGGGGGGAACCTGGGGACCGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAATACTGGCTGGCGCGATATCAGACGCCGCAATCATCGTTTACAGTCCGGCATTGATGCCCGTGCTTCATGTACTGGATTGGGTGCCGGTTCGTCCTTGCGGGTGTGGTATATGCCTTGATCGGTCATGGTGAGAAAGCGTTACCGGCCTGGTTTGCTTCGCTGGGGTGATTTCCATACTTCGCAGTATCAGGTGTTAACGCGTCTGGCGCAGTTCTCTCTGGCGCGTGAACCGCATGTCGATAAGGTGGAGACGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTTGGTGTAAAGATTATTGCCCTCACCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCCATACCGGGATCAGGTATACCGAAATCGCCATTCCTGGGGTCATCAGCATAATCACCAGTACCGCCGCCATAAACAGCAGGCAGATCCAGTTACCCAGCGGATAAAGCAGAGCAGGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCGCCCAGTTGATTACCAGTGCAGATACCACCAGCGCCATTAACAGTCCGAAAGCGGACTCTGGGGCAAGGTAGTTAATCAGTACGCACAACGCGCGTTACCAGTGCAGACACCAGAATGGTATTTACTGGTACACCACGTTTATCGACAGACGCCAGCGCTTTTGGGCGCATTACCCTGTTGTGCCAGACCAAACAGCATACGGCTGTTGCAATATACGCAGCTGTTGTACACGGAGAGCGCCGCAGTCAGTTACCACGATGTTCAGCGCATTCGCCACAAAGGTATCGCCTAACTCGTGGAAGATCAGCACAAACGGACTGGTATCGGCGGTAAACGCGGGGTCCACGGCATCAGTGAGAGCAGAACGGCTAACGAACCAATATAGAAAATCAGGATGCGGTAGATAACCTGGTTAGTTGCTTTCGGTATACTTTGCTCCGGGTTATCAGCTTCTGCTGCGGTGATCCCCACCAGTTCCAGACCACCGAACGAGAACATGATAATCGCCATCATCATCACCAGCCCGGTGAAGCCGTGCGGCAGGAAACCACCCTGATCCCACAGGTTGCTAACGGTCGCCTGCGGGCCGCCGTTGCCACTGAATAGCAGCCAGCGCCGAAGATGATCATCGCTACCACCGCGATAACTTTGATAATGGCAAACCAGAACTCCATCTCGCCAAACACTTTAACGTTGGTCAGGTTGATGGCGTTAATCACCACAAAGAATACGGCGGCAGAAACCCAGGTGGGGATTTCCGGACATACCCAGAACTGAATGTATTTACCCACGGCAGTCAGCTCAGCCATGGCAACTAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATCTCACCCAGCTGACGCATGATCAGAAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGATAATCCCTGGCCTGCGGACTGTATTACGGAGGCGCTACCCAGGAATAACCCGGTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGCGGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCATGAAACCTCGTGCGGGGTTGTTTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTATTAATTGTTTTACATCAAAGAAGTTTGAATTGTTTACAAAAAGACTTCCGTCAGATCAAGAATAATGGTATGCGGCAGCGAATGCACCCGCTTTATGCATGGTTGAAGATGAGTTGCTTAAAAAGAAAACCGTTTTGTAAAGCTCAGCTCAACCCCTCTCAATATGTAGAATGAATTAAAATTCGTTTTAATTGAATTAAAAATCACAAAATTGGTAAGTGAATCGGTTTCAATTCGGCTTTTTATAGTTTAATAATCGTTAAAAAATCTCCTTTCCTACGTAAAGTCTACATTTGTGCATAGTTACAACTTTGAAACGTTAATATATGTCAAGTTGTTTAAAATGTGCACAGTTTCATGATTTCAATCAAAACCTGTATGGACATAAGGTGAATACTTTGTTACTTTAGCGTCACAGACATGAAATTGGTAAGACCAATTGACTTCGGCAAGTGGCTTAAGACAGGAACTCATGGGCCTACAGCAAAATCCGCCCAACCAAAACTCTTCCGATGTGATTTGAGCAGCAACTGGAGTTTTTGATGCCTCGAAGGCACTCTCCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGTCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAAGGGTGGCGGCACTTTTGTCCGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCCTGAGTCACAAGTTATGACTTGCTCGAAAACGACACGCCCCTGGAAGGTATCGCCGCTTATTACGCCGCGGCTGCGTAGTACCGATGAAGACAAGGAACCATCCGTGAACTCCACCACGCCATAGAGCTGGCGCAGCAGTCTGGGCGATCTGGACGCGGATCAAACGCCGTACTCCAGTATCAGATTGCCGTCACCGAAGCGGGCCACCACAAATGTGGTTCTGCTTCATCTGCTAAGGTGTATGGAGCCGATGTTGGCCCAGAATGTCCGCCAGAAACTTACGAATTGCTCTATTCGCGTCGCGAGATGCTGCCGCTGGTGAGTAGTCACCGCACCCCCATATTTGAAGCGATTATGGCCGGTAAGCCGGAAGAAGCGCCGCGAAGCATCGCATCCCCATCTGGCCTTTATCGAAGAGAAATTTTCTCGACAGAAGTGTGAAGAGAGCCGCCGTGAGCGTTCTCTGCGTCGTCTGGAGCAACGAAGAATTAGTTGACTTTTTTTCTGGGTAAAATTATCCAGAAGATGTTGTAAATCAAGCGCTATAAAAGCGCGGCAACTAAACGTAGAACCTGTCTTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAACTCAACGTTATTAGATAGATAAGAAATAACCCCAGTCAGAAACGTTTCCCAAATGACGTGGATCCGATCGAAACTCGCGACTGGCTCCAGGCGATCGAACGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAGCCGCAGGCACAGGTATCAGCAACTACATCAACACCATCCCCCGTTGAAGATACACCGGAGTATCCGGGGAATCTGGAACTGGAACGCCGTATTTCGTTTCAGCTATCCGCTGGAACGCCATCATGACGGTGCTGCGTGCGTCGAAAAAAGACCTCGAATGGGCGGCCATATGGCGTCCTTCCAAGCCTTCCGCCAACCATTTATGATGTGTGCTTTTAACCACTTTCTTCCGTGCACGCAACGAGCAGGATGGCGGCGACCTGGTTTACTTCCAGGGGCCACACATTCTCCCCGGGCGTGTACGCTCGTGCTTTCCTGGAAGGTCGTCTGACTCACGGAGCAGCTGGATAACTTCCGTCAGGAAGTTCACGGCAATGGCCTCTCTTTCCTATCCGCGACCCGAAACTGATGCCGGAATTCTGGCAGTTCCCGACCGTATCTATGGGGTCTGGGTCCGATTGGTGCTATTTACCAGGCAAATCCTGAAATATCTGGAACACCGTGGCCTGAAAGATACCTCTAAACAAACCGTTTACGCGCGTTCCTCGTGACGGTGAAATGGACGAACCGGAATCCAAAGGTGCGAATCACCATCGCTACCCGTGAAAAACTGGATAACCTGGTCTTCGTTATCAACTTGTAACCTGCAGCGTCTTGACGGGCCCGGTCACCCGGTAACGGCAAGATCATCAACGAACTTGGAAGGCATTCTTCGAAGGTGCTGCTGGAACGTGATCAAAGTGATGTGGGGTAGCCGTTGGGATGAACTGCTGCGTAAGGATACCAGCGTAAACTGATCCAGCTGATGAAGAAACCGTTGACGGCGACTACCAGACCTTCAATCGAAAGATGGTGCGTACGTCCGTGAACACTTCTTCGGTAAATATCCTGACAAACCGCAGCACTGGTTGCAGACTGGACTGACGAGCAGATCTGGGGCACTGGAACCGTGGTGGTCACGATCCGAAGAAAATTACGGCTGCATTCAAGAAAGCGCAGGAAACCAAGAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACCAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGGCGACCGTTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGCATACCTATCTGCACGCTCAGCGTCAGGAAACTGCACGGTTATCTGCCAAGCCGTCCAGCCGAACTTCACCGAGAAGCTTGAGCTGCCGAGCCTGCAAGACTTCGGCGCGCTGTTGGAAGAAGCAGAGCAAAGAGATCTCACCACTATCGGCTTTCGTTCGTGCTCTGAAACGTGATGCTGAAGAACAAGTCGATCAAAGATCGTCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTATGGAAGGTCTGTTTCCGTCAGAATTGGTATTTACAGCCCGAACGGTCAGCAGTCACCCCGGCAGGACCGCGAGCAGGTTGCTTCTATAAAGAAGACGAGAAAGGTCAGATTCTGCAGGAAGGGATCAACGAGCTGGGCGCAGGTTGTTCCGGCTGGCAGCGGGCGGACCTCTCTTACAGCACCAACAATCTGCCGATGTCATCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTGGGGCGATCTGTGCTGGGCGGCTGGCGACCAGCAAGGCGTGGCTTCCTGATCGGCGGTTACTTCCGGTCGTACCACCCTGAACGGCGAAGGTCTGCAGCACGAAGATGGTCACAGCCACATTCAGTCGCTGACTATCCGAACTGATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACATCACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGGGCTCCGGTTCTATCCTGCGTCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATAAACTTTCGCCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGTACTGATTGGCTTCGGTCGTTCCGACAGCCGTGGAGAACCTGCGTCACCACTTCGAAGTTGATGCTTCTTATGTCGTGGTTGCGGCGCTGGGCGAACTGGCTAAACGTGGCGAAATCGAGATAAAAAGTGGTTGCTGACGCAATCGCCAAATTCAACATCGATGCAGATAAAGTTACCCGCGTCTGGCGTAAGAGGTAAAAGAATAATGGCTATCGAAATCAAAGTACCGGACATCGGGGCTGATGAAGTTGAAATCACCGAGATCGCCTGGTCAAAGTGGGCGACAAGTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGCAAAAGCACTCTATGGAAAGTTCCGTCTCCGCAGAGCGGGTATCGTTAAAGAGGATCAAAGTCTCTGTTGGGCGATAAAAACCCAGACCGGCGCACCTTGATTATGGATTTTCGATTCCGCCGACGGTGCCAGCAGACGCTGCACCTGCGTCAGGCAGAAGAGAAAGAAAGAAGCAGCTCCGGCAGCAGCACCGCGGCTGCGGCGGCAAAAAGACGTTAACGTTCCGGATTCGGCAGCGACGAATTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACGAGTCGCCGATCACCGTAGAAGGCGACAAGGCTTCGTTGGAAGTTCCGGCTCCGTTTGCTGGCCCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAATCGGGGTGAAGCAGGCGCGGCAGTCCGGCCGCTAACAGAAGCAGCTCCGGCAGCGGCCCCTGCACCAGCGGCTGGCGTGAAAGAAGTTAACGTTCCGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCCTGAACAGTCACTGATCACCGTAGAAGGCGAAAAGCTTCTATGGGAAGTTCCGGCGCCGTTTGCAGGCGTCGTGAAGGAACTGAAAGTCAACGTTCGGGCGATAAAGTGAAAACTGGCTCGCTGATTATGATCTTCGAAGTTGAAGGCGCAGCGCCTGCGGCAGCTCCTGCGAAACCAGGAAGCGGCAGCCGCGGCACCGGCACAAAAGCCTGAAGCCCGGCAGCAGCACCAGCTGCGAAAGCGGAAGGCAAATCTGAAATATGCTGAAAGACGACGCTTATGTTCACGCGACTCCGCTGATCCGCCGTCTGGCACGCGAGTTTGGGTGTTAACCTTGCGAAAGTGAAGGGCACTGGCCGTAAAGGTCGTATCCTGCGCGAAGGACGTTCAGGCTTACGGAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCGTAACTGGGTAATGACCCGCATGTTACTCACTTTCGACAAAACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCGGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGAAAGCCGTTGCTGCTAGCTCTTGAGCAGATGCCTCGCTTCAAATAGTTCGCTGTCGGAAGACGGTCAGCGTGCTGACCCTGAAGAAATACATCAAACATCGGTGTGGCGGTGGATACCCCGAACGGCCTGGTTGTTCCGGTATTCAAAAGACACAACAAGAAAGGCATCATCGAGCTGTCTTCGCGAGCTGATGACTATTTCTAAGAAAGCGCGTGACGGTAAGCTGACTGCGGGCGAAATGCAGGGCGGTTGCTTCACCATCTCCAGCATCGGCGGCCTCGGTACTACCCACTTCGCGCCGATTGTGAAACGCGCCGGAAGTGGCTATCCTCGGCGTTTCCAAGTCCGCGATGGAGCCGGTTGTGGAATGGTTAAGAGTTCGTGGCCGCGTCTGATGCTGCCGATTTCTCTCTCCTTCGACCACCGCGTGATCGACGGTGCTGACTGGTGCCCGTTTCATTACCATCATTAACAACAACGCTGTCTGACTTCGCCGTTGGTGATTAAGTAAAAGAGCCGGCCCACGGCCGGCTTTTTTCTGGTAATTTCATGAATGTATTGAGGTTATTAGCGAATAGCAAATCGGTGCCGTTTGTTGTTTAAAAATTGTTTAAATTTTTAAAATTACCGACGGTAGAACGACCCCGGTGGTGGTAGGGTATTACTTCACCATTACCCTATGGATTTTCTGTGGTGCAGGAAGGTAGCAAGCGCCCAGAATCCCCCAGAGCTTACATAAGTAAGTGAACCTGGGGTGAGGGCGTGAACTAACGCCGCTGCGGGCCTGAAAGAACGACGGGTATGACCGCCGTAGATAAATATATAAGAGGTCATGATGAGTTCTGAAATCAAAAACTCGGTGGGTACTTGGGGCAGGGCCCGCAGGTTACTCCGCTGCCCTCCGTTGCGTGATTTTAGGTCTGGTAAACCGTAATCGTAGAACGTTAAACACCCTTTGGCGGTGTTTTGCTGAACGTCGGCATGTATCCCTTCTAAGCAGCCTCTGCACGTAGCAAAAGTTATCGAAGAAGTCCAAAGCGCTGTGAACACGGTATCTCTTCGCGAACCGAAAACCGATATGTACAGATTCGTATCCTGGTAAGCAGAAAACTGCATCAATCAGCTGACGGTGGTCTGGCTGGTATGGGCGAAAAGGCCAAAAGTCAAACGTGGTCACGGTTACTGGTAAATTCACCAGGGGCTAACACCTGGAAGTTGACGGTGAGAACGAGCAAAAACCTGATCAACTTCGACAACGCGATCATTGCAGCGGGTTCTCGCCCGATTCCAACTGCCGTTTATTCCGCATGAAGATCCGCGTATCTGGGACTCCCACTGACGCGCGGATAGTGAAAGAAGTACCAAACGCCTGCTGTAATGGTGGGCGGTTACTTCGGTCTGGAAATGGGGCACCGTTTACCACCGCTGGGTTCACAGATTGACGTGGATTAGAATTGTTCGACAGGTTAATCCCGGCAGCTGACAAAGACATCGTAAAAGTCTTCACCAAGTCGTATCAGCAAGAAATTCAACTGATGCTCTGGAAACCAAAGTTACCGCCGTTGAAGCGAAAGAAGACGGCAATTTATGTGACGATGGAAGGGCAAAAAAGCACCCGCTGGAACCGCAGCGTTACGACGCCGGTGCTGGTAGGCGATTGGTCGCTGTGCCGAACGGTAAAAACCTCGACGCAGGCCAAAGCAGGCGTGAAAGTTGACGACCGTGGTTTGTCATACCAGTTGACAAACAAGCCTGCGTACCCCAACGTACCGCACATCTTTGCTATCGGCGGATTATCGTCGGTCAACCGATGCTCGCACACAAAGGTGTTCACGAAGGTCACGTTGCGCTGGAAGTTATCGCCGGTAAGAAACACTACTTCGACCGAGAAGTTATCCCGCCATCGCTATACCGAAACCAGAGTTGCATGCGGTGGCGTCTGACTGAGAAAGAGCGAAAAGATGAAAGGCATCAAGCTATGAAACCGGCACATCCTTCCCCGTGGGCTAGCTTCTGGTCGTGCTATCGCTTCTAGGACTGCGCCAGACGGTAGTGACCAAGCTGATTTTCGACAAATAATTCTCACCGTGGTATCGTGGTGCCGATTGTCGGTACTAACGGCGGCGAGCTTCTGGGGTGAAATCGCCTGGCAATCGAAATGGGGTTGTCGATGACTGAGACATCGCCTGACATCCATCGCGCACCCGACCTCATGCACGAGTCTGTGGGGCCTAGGCGCAGGAAGTGTGTCGAAGGTAGCAATTACCACCTGCTGAACCCGAAAAGCGAAGAAGAACGTAATTTTCGTTTGCCGGAACATCTGGCAATTAAAAAAAGCGGCTAACCACGCCGCCTTTTTGTAACGTCTGCCAATTTACTTTCCAATTCTTCTTGCTCCACGTTCAGAGGAGACGTTCGCATACTGCTGACCGATGCTCGTTTTCAGCCGACAGTATGTTACTGTTCGTTAGACGTTGTGGGCGGCTCTACCCTCTGAACTTTCTCCCGGAATAAGAAAAACCTGTACGTTGTTCAAGGGTGCATGGCGATTGAACACGCTGGCGCGGGCGTTATCACGTTGCTGTTGATTTCGTGGGCGCGGCTGTACTTTTTCTTAAACACCTGGGCGCTGCCCTCTGTGTGGGACTTGAATGACGCTACGCGCTGCGTCTTCTCTCGCGGCTGGTTCTGCGGGTTTATCTGCATTTTCTCGCGTAACCGCCTGGCGGCTGCGCTTAAGGGCGAGGCGGACTGAATGCGCTCACGCGCTGCCTCTCTCTCGCTGCTGGATCTTGGGTTCAGTCTGCATTCTCCGCGGAACTCCGCGCTGCTCAGGCGAGGCGGACTGATAACGCTGACGGCGGCGTCCCTTTTTTGCCTGGGTCAGTGGTATGGCGACGGCTGAAGCGGGAAGTCTCATAGCCCCATAGTTTCAGCTTCATTAAACCGTACTGCCGCTGCCCCTGACGATTTGGGTATCTCGTGTAATGACTGGTGCGGCGTGTCGTTCGTTGCTGAAACTTGATTTGCTGGCCCCTGACGCTGGCTGTCAGCGTATGGGGGCAGGTAATTGGCGTGGCGCTCATTCTCCGTTGACACTGGTTTGTGGGAAACCGCTTTTGCCATATCCTGATCATGACTAGGCACACATTCGGTTAGCTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATTGCTCCCGGGGTGATACGGTTGAAATTGTTGGACGTCGATATTATGTTGTCCCGTTGTTGTCTGCCAGCATTTTCCTCACGATGACCGCCAATATCGTGGTGATTGTAACATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTACGTGGTAGCAACGCCCATGCTAAGCCAATCCGCGTACAAGCGTCAACAACGGTTCTCCGGCTGGTGGTGGCAAGATAACCGCGGATCGCAGTAATTGGCCCAGTTCCCGTAAACCACGGGTTGGTTGTAGTTGGGAAATATAGACCACATCAGGATTGGCGGCTCAATGGAAATGACGTTGTTGGCCGGCTCTGTAATGCGAGGGTTGGCAGTTAAAACTGTTGGATCTGTATGACGGGTTGCGTACTGTCCTGTTTTACCGGTAAGCTTTCTTCGTTTGGGGTAATAACTTCTGTTCGTGATGACTCAGCGAGCGGTCTTGTTGCGCCAGTTTGCCGCAATCCCTGTACCGAGTCCATCACGTCCTGCGGCTGAGCCAGAAAAGCATCGCCCAGGTTTTGCCCCATTGCGGGTTTTCCCCATCATGCACAATGTGGTAAAGGCCCACCAGTGATTTAACGCTGGCGTCCCACGGCTGGGTCAGATACCGCTAATAGGCAGCATCGCCTTGTTTTGAAGTGGATTCACGTGCGACCATTGCACTGTTGAGTACGTTGTGGAGTAGGTTTTTGGATGCCATCACACCTGCGAAAGTAGGGCGTCGTGGGAACGCGGACGGGCGCGACCCATTTGATCTATTTGTGCAGTGCCGATATGCGGATTTTGACGGACGGCGGTGTTACGGGAGGTGCCGTAAACCGTTTCGCAGGAAAGTACCCGGGCAGCGACAGATCACAGGCCAGTTGTGTCGCATTGCGGTTCC +>contig_2 LN:i:13560 RC:i:8 XC:f:0.750000 +GCGAAAATCCTGGAAAGAGGCGGTGCCGCCGGTCCGTTTGCCTTCCCGGCTCCGTCGCAAATGTTTGAAAGCGATGGTCGGTTGGTCTGGAATTGTTCCACGGGGGCCAAACGCTGGCTATTTAAAGATTTCGGCGGTCCGCTTATATGGCACAAATGCTGACGCCATATTGCGGTGGCTAAGCCAGTGACCCAATTTTCTGACCGCGACCGCCGTGACTACCGGAAGCAGGGCAGTTGGCTCCAGCTTTCGTACGGTTAACCGAAGTGAAAAGGGTATATCCTCCATCTCACACGAAGGCAAAAAAGTCAAGTCCCACCTGCAAAGAAAAAAACTGTTCTGTACATTGGGCGGGAATATTCACGATAAAACTCGTTGGCCAATCGGACCGGCGGTATCTCGTTAGCCTTGTTGCAAGGCGCTGGTGGATAGGCAGGCGTTTGATGGATGGATGAACTGAAAGTGCGCGCTAGGGGGCTTAAACTCGGGCTAACTGGATTAGACATACTAGCCGTTTGCCTGGGCGGCAGATTTTTGCTAGCCTGACTTTTGGAGCTGTTTGCGCAGCCGCCGAGGGCAGGAGCGCGCTGCGCAAGCCGCTGGGTTGTCTCCGGTGCCAAGGCGGGAAACGTTCGGCGCTTTTGACGGCGGGTTCTGCTGGGGCGAAAGTCCACGTGGCGGTCTGCCCGGTTGAAACCTGTTTGTATTTGCTGCGACGAGACGGTGAACGAATACCGTGCCACGGTTTCCTGACACGAGGGCAGTGGGTCCAACCCCAAAGGGACCTCAGGCGACGGTTATCCAAACGCGATGGACGTGGCAGGCTCGCCGAACAAACCTGGCCGCTGCTGTGGAAAGAGTTCTTCACGCCGCCAAAAATCTGGGCAAACTGGAAAGATGCTGGGGGTTATGCAGCCGTGGGTATGATGAAACCACTCGCAACAGACAATGGCGGTGAGGTTAAAAGAACGGGGCTACCACCTTCGGAGCCGCTACGCGCCTGACCGTTGTAGAGCTACGATGCGCTGCGTTGTTCAGTTGAATCCAGGCGCAATATTGGCTTGTTCCTCGGGCACCGCCGAACAATCCCCGGCGAAAATTTAAAGGAAGAGCGTGGAAGCGATTTCTGGGGTGAAACGTTGGATACTGGCCAAAAGGAGCTGGGCAGAACGTGGCCTTGAATTACCCCTTGCTTTTCAACAATAATCTGCCCGCCGATTTTTCGCTGCGTTCGTGCGTAAATTGATTGATGAATCATCCGAGTAGAAATCTATTGTCCACTAGTCCTTCAAATCCAGGCCGGGGCTGCTTTTATGCAGCCCGGCTTTTTTTATTGAAGAAAATTACGGAGAAAAAATGACGAGCGGAAAGAGGAGAAATTCCTCCAGTAAATGCGGTAACTTAGGAGATTTTAGGAATTGCGGAGACATTAACAACGGCCGGATCTCATCGGGTACCATCTCCCGGGTAAGCCCCGACCCTAATAGCGGGCAATGATAAAATGGAGTAGACCTGTGAAAAAGATGTCCAATCTATCGGTTCTCGCCACCTTTCCCCTCTGGTTCTGGTCGCCTCCCCATGGCAGTTCGCCCAGAGGCATGCGGAGATTACCGTTTAGTCCCGTTCAGTAAAATTACAGAATTAGGGGAATCGTTGCATAATCGTGGCTTATACATGGGATTGGAAGGTCACTGGCGCCGACCACGGCTGCGATGGAAACAACCTTTAATGAATGGCGAGGCAATCGCTGGCACCTACACGGACCGGCCGCCCACGGCCGCCGACCACCATATTAGATGAAGCTCCTCAATGATTTGCATCACGGCGGTCATGGTTCCAGTGGGCAAAATCGACCGCTAATGTGACACATGCCGGGCAACAAATCCGGCATTCAGCCGGCCTGATGACGACGGCTGGCGCGCCGCCCCTTATCAGGTCCTACCGTTAATTCTCTTGCAAATAAATTATTCTTGAAATCATCGCCATGCCTTTTCTGTAGGCGCAAGGATATAAGTGCCGTTTCGAACGCCGCTTATCCCCGGCGATTGAGCTGCGAACTTAAACGGCCTGCTCGGTAGCGCTCTAAACACCAGAGTTTCGCCAAGTTGCTGGAGGAATCTTTCATCCAAAGTATGTAAACACCTATCGCTATTAAAAACCAGTCAGTGTGGCTCTGCGTTGGTCAGGCCGAATTTTGCAACATAATGAAACGGACTCACCGAGCATCCCCGCGTGCGTTTCCTTAGGTCGGAAGCGATGGGTCTTAAAGTTGTCCGTTCTTTCCTCATCCGGAGGCACACACGGCTGGGCTGTAGGATAAACTTACGGCATTGTCAATTTTCTCTCGGGCTTCACCGGAATCAAAATACTCACCTGACCGCCAGATTAAATCACCCAGCTTATGCGCCCTTGGTGCTGCGGGCGCGCCTCGTTCCAGCTTGTTGGTGATTGATGTATCTCCCCCGGAAAGTTTTGAATGCACAGAATCTTTCCGTCGGGCATTCTCAAGGCGGATCCCCCATTTCCAGAACGATAAGGGTGCATCTGAAATCGTACGCGGGCGGAGTCCGGCCAATAACCAAGCCGGAAAGCATTACGCAAAATGCTGTTGGGAGCAAAACAATCGGAAATCGTGTTCGCTGGAGGTTTCGGCCTCGCAAGCCCGTGTTTAGACTTCAACCTTTAAACGCCAGAATCCGCCTTTGGCGGGCCCATTCGCCGGGCGTTTGAAATCTGGCCTGCGAGTTTCATGCCATAGCGAGCGGCGCTGGAATACCCGCCCAGTTTTGTCGCTGATGCGCATCAGACGTTGCCCTGACTACTTCGCGGAGGGCGTCACAGCTCTGTTCCGCCTGCCTCATTGGATCAACTGCTGGGAATGGGCCATACAGGCCTCCGGCAGCGTATTGAGCGCGTGGGGGTCCAAACGGGGGGGCTTTGCGGTATTCTAAGCTGTTTTTCGCCAGGGGACAATAAGAATCAGCATAGCCCAGTCCCCTTGCAGGAAAATTTATGCCGCCTTTGCAAAAAAATGAGAATGAGTTGATGATAGTTGTGATTACTCCGGGAAACAATCATCCCACGCGTCCGAGAGAAGCTGGCGACCGATATCCGGATAACGCAATGGAATCAAACACCGGGCGCACGCCGAGTTACGCCTGGCGTAGATAATCACTGGCATGGTATGGAACACAGGCGAGAGCAGGTAAAATGGCGGTCAAATTGGTAAAAGCCATGCAGGCAATTATGATATCTGCCAGTTGCCACATCAGCGCGAAGGGCTTACAAGGGTGCCGCCGATGACGTTGCGAAGGTGCAGAATCCGCAAACACCCAGATCGCTTTTAGGGTTTGTTCAGGCGAAAAAGAGAGAGCATTGTTTTCGGCAATAAATGTAGTTGCAACGATGGAGCTGAAGGAAAAACAGAATAACCACAGAGGGAACAACACTCAGCACCCCAGCAACCCTTAGCTCCCCTCGATCGCCTTCTGGATAAGCTGAATCACCTTCAGCGGCATGTAGGTTGTGTGCGTTACCCGCCAGTAATAATCAGATGGCGCTTGGCCTACAGTGACCAAGGGTTCGATAAAAATGCCAATCATCTGGACAATCCCTTGCCGCTCCGGATGCGGGAGGCCAGGACGCCGCTGCCGCTTGCCGCGTTTGGCTCGAACCCATTCCCGCCTCATTGGAAAAACATACTGCGCTGAAAAACCGTTAGAATCGCCTGGCTTAAGGTATATCCCGCCCGCGCCGGCCTGCCGCTTCCTGCCGCCAAAAGCACTCTCAAAAATAGAACAAATGACGTGGGGAAGTTGCCCGATATTCATTACGCAATTACCAGGCTGGTCAGTACCCAGATTATCGCCCTCAAACGGGACAAAGCCCTCCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATTGCCAGCAGAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAACTCAGGGCGCGGGCAACGGCGTTCGCCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAAGACGGCGAACAGAAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATGACGCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAAAAGACGGCTCCAGGTCCACCGGCGGTCAATAGCCAGCGCAACGCCGGCCAGGTTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACACATGACTGAAATGAGCTTAAACCGCTGGCTGTGGATGAATGCTATTTTTTAAGACTTTTGCCAAACTGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTTACCGATCCCCCAAAGGACGCTGTTAATGGAGGAGAAAAATCTGGGCATGCATATCCCTCTTATTGCCGATCGCGATGACTTTCCTGTGGTAAACGTTACCAATTGTTTAAGAAGTTATATACCTACGGGAGGTACTTTGATAACTTCTGCGTTAAGCCTACATGAGGTTTTGTATTAAAATGGCGGGCCGTATCAACGCAGTGTCAGAAATCCGAAACAGTCTCGCCTGGCGATAACCGTCTTGTCGCGGTTGCGCTGACGTTGCGTCGTGATACATCAGGGCAGACCGGTACAGTCCCCCTAACAATCTGTTTAAAGAGAAATACTATCATGACGGGCTAAATTGACCTCCCTTCGTCAGTAACCACCGTAGTGGCCGCACTGGGGACATCGCGGCAATGAAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTTCATTCTTAACGCAGCGCAGATTCGGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGGGCGCAGCAGATCGTGGACGCGACCGCAAACTGGCAGGTTAAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGGTCAATTGCGAAAGCAAAACGCCTGATCAAATCTACAACGACTGCTGGTATTAGCAACGATCGTATTCTGATCAACTGGCTCTACCTGGCAGGGATCCGTGCTGCAAGAACAGCTGGAAAAAAGAAGGCATAACTGTAACCTGACCCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGCTGCGGAAGCGGGCGTGTTCCTGATCTCTCGCCGTTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGGATAAGAAAGAGACGCTCCGGCAGAAGGATCCGGGCGTGGTTTCTCTATCTGAAATCTACCAGTACTACAAAGAGCAGGTTATGAAACCGTGGCTTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCAACTGCTAAAGAGCTGGGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTGTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGAAAAATGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTCGGTCACGCTACCTCTTCTGAAGCCTTTCTGTCACTCCCTTCGCAGTGTATCATTCTGTTTAACGAGACTGTTTAAACGGAAAATCTTGATGAATACTTTACGTATTGGCCTTAGTTTCCATCTCTGATCGCGCATCAAGCGGCGTTTATCCAGGATAAAGGCATCCCTGCGCTGGAAGAATGGCTGACATCGGCGCTAACCACGCCGTTTGAACTGGAAACCCGCTAATCCCGATGAGCAGGCGATCATCGAGCAAACGTTGTGTGAGCTGTGGATGAAATGAAGTTGCCAATCTGGGTGCCACCACGGGCGGAACTGGCCCGGCGCGTCGTGACGTAACGCCCGATGCGACGCTGGCCAGTAGCGGACCGCGAGATGCCTGGCTTTGGGAACAGAGCGCCAGATCAGCCTGCATTTTGTACCAACTGCGATCCTTTCGCGTCAGGTGGGCGTGATTCGCAAACAGGCGCTGATCCTTAACTTACCGGTCAGCCGAAGTCTATTAAAGAGACGCTGGAAGGTGTGAAGGACGCCTGAGGGAACGTTCTGGTACACGGTATTTTTGCCAGCGTACCGTGACTCATTCAGTTGCTGGAAGGGGCAACGTTGAAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTTCAAGACGCGACGTTAGCGAATAAAAAAATCCCGCCCGAGCGGGGGGATCTTCATAAACAATTAGTGGGATTTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCGCTGGCACCGCAGATCAGCCCATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCCGGCAATGTTACGATCGCCAGCAGGGCAACAGCACGGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTCAGCGTGCCGAAGAACATAAACAGCGTAATACGGCCCCCACAGACCCAGGTAGACACCAAAGGAACTGTGCATTTGGCGCATTCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAGCGTCAGCCAGAAAGAACCGAAGAGGTGATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGCCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAAGTGCCCATTGGCAAGAATAATACCGTCCAGGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCACAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCTGAAATAACTGTAGTGTTTTCAGGGCGCGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGGGGGAAAATGAAAATTTTTCCCCGGTTTCCGGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTAGTGCCGCACCTACAAGGGCCTCCCAGCCCCGCGCCGCGCAGCAAACCATGCCCAAGTACGCTCATTGCTGCGTGGGGTGCGTAAAATGCGGGGTCAGTTGGCTGGAAAGCAAATGCGACACACCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATTTTCTTCCAGGTATTACCGCAGAAATTGTTTCCAGTAACTGTTGCTCAATTTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGCTTTGAAACATCGAGCAAAATGGCCCGATACACATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCGGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGTTCGTTGCGCATCAGTACGCTGGAGAGGCGAGTTTTTGCCTTTTTTCATTATGGGTGAGCAATCGGGGCGAAATTTGCCAACTGTTCCTCACTACAATGCTGAAGAAAATCCAGATCTGAATCATTCAGGTAATTTAACATTCATTTTTTGTGGCTTCTATATTCTGGCGTTAGTCGTCGGCCGAATAATTTTCAGCGTGGCCATATCCGATGAGTTCACCGTATGACCCGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTATCGCTGTTAATGTTGATCCAGTCAGTGGTTTGCCCCTTCTTTATTTCTGAAGGAATATTCAGGCTCTGACTGGCGCTACGGGCGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGGAGTTTATGTTCGTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTTGGGAAGAGGTAGGGGGATGAATACCACTAGTTTACGCTGATAAAGAGAAGATTCAGGCACGTAATCTTTTCTTTTTATTAACAATTTTTTTGATGAATGCCTTGGCTGCGATTCATTCTTTATATGAATAAAATTGCTGTCAATTTTACGTCTTGTCCTGCCATATCGCGAAATTTCTGCGCAAAAGCACAAAAATTTTTGCATCTCCCCCTTGATGACGTGGTTTACGACCCCATTTAGTAGTCAACCGCAGTGAAGTGAGTCTGCAAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCTATTACAGACTCACAACCACATGATGACCGAATATATAGTGGAGACGTTTAGATGGGTAAAATAATGGTATCGACCTGGTACTACCAACTCTTGTGTAGCGATTATGGATGGCAACCACTCCTCGCGTGCTGGAGAACGCCGAAGGCGATCGCACCACGCCTTCTATCATTGCCTATACCCAGGATGTGAAATCTGTTGGTCGCGGCTAACGTCAAGGCAGTGACGAACCCGCAAAACACTCTGGTTTGCGATTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGCATGGTCGAAGTTAAAGGGCCAGAAATGGCACCGCCGCAGATTTCTGCATGAAGTGCTGAAAAAAAATGAAGAAAACCCGCTTGAAGATTACTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAATAAAACGTATCATCAACGAACCGACGCAGCTGCGCGGCTTACGGTCTGGACAAAGGCACTGGCAACCGTACTATCCGGGTTTATGACCTGGGTGGTGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGGTTGACGGCCAAAAAACCTTCGAAGATTCTGGCAACCAACGGTGATACCCACTGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATCAAGAAAGATCAGGCATTGACTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCAGACGCGACCGGTCCGAAACACATGAAACATCAAAGTGACTCGTGCGAAACTGGAAGCCTCGGGTTGAAGATCTGGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGCCTGTCCGTATCTGATATCGACGACGTTATCCTCGTTGGTGGTCAGACTCGTATGCCAATGGTTCAGAAGAAAAGTTGCTGAGTTCTTGGTAAAGAGCCGCGTAAGACGTTAACCCGGACGAAGCTTGAGCATCGGTGCTGCTGTTCAGGGTGGTGTTCTGACTGGTGACGTAAAAGACGTACTGCTGCTGGACGTTACCCCGCTGTCTCTGGGTATCGAAACCATGGGCGGTGTGATGACGACGTGATCGCGAAAAACCACCACTATCCCGACCAAGCACAGCCAGGTGTTCCTACCGCTGAAGACAACCAGTCTGCGGGTAACCATCCATGTGCTGCAGGGTGAAACGTAAAACGTGCGGCTGATAAACAAATCTCTGGGTCAGTTCAACCTAGATGGTATCAACCCGGCACCGCGCGGCATGCCCAGATCGAAGTTACCTTCGATATCGTGCTGACGTATCCTGCACGTTTCCGCAAAGATAAAAAACAGCGGTCAAAAGAGCAGAAGATCACCATCAAGGCTTCTTCTGGTCTGAAGAAGATGAAACCAGAAAATGGTACGCGACGCAGAAGCTAACGACCGAAGCTGACCGTAAAGTTTGAAGAGCTGGTACAGACTCGCAACCAGGGCGACCATCTGCTCACAGCACCCGTAAGCAAGGTTGAAGAGAAGCAGGCGACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGGCTGACATTGCACTGGAAACTGCTCTGAAAGGTTGAAGACAAAGCGCTATCGAAGCGAAAATGCAGGAACTGGCACGGGTTTCCCAGAAACTGATGGAAATGCCCAGCAGCAACATGCCCAGCCAGCAGACTGCCGGTGCTTGATGTTCTGCAAACAACGCGAAAGATGACGATGTTGTTGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACCGCAAAAACGTCGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAACAAGGTGGCATGGGCGGCGGTGGGTTTTTTGGCGGCGGGCAGACTTCAGCGATATTTTTGGTGCGTTTTCGGCGATATTTTTGGGCGGCGGACGTGGTCGTCAAAGTGCGGCGCGCGGTCTGATTTACGCTATAACATGGAGCTCACCCTCGAAGAAGCTGTACGTGCCGTGACCAAAGAGATCCGCATTCCGCTCTGGAAGAGTGTGACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACTTGACCAACCTGTCATGGTTCTGGTCAGGTGCAATGCGCCAGGGGATTCTTCGCTGTACAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTGAGCGCAGCAAAACGCTGTCCGTTAAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTGCGGGCGAGGTGAAGCGGGCGACATGGCGCACCGGCAGGCGATCTGTACTTCAGGTTCAGGTTAAACAGCACCCGATTTTCGAGCGTGAAGGCAACAACCTGTATTGCGAAGTCCCGATCAACTTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTATTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGGTGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAGACGAAAGGCGAAACAGGCTGCGCAAAGCTGCAAGAAGCTTCGTGGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTTGACGACCTGACCCCGCTAACCTCCCCCCAAAAGCCTGCCCGTGGGCGGCCTGGGCTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGAGATCACTCCCCATAAGCGCTAACTTAAGGATTGTGGTATTACGCCTGATATGATCTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTCGGCACGTAATGCCGGGCTCTAACCCGCCGCCGCGAAATTCGTGATGCTGCAACTCTGCTACGCTCTGGGGCTGGCTTACGGCCCCGGGGGATGTCATTAACGTGAATCACTGCATGGGCTCAGCTCCATGACGTTGCAACATTATCTGACGTGGCTCTCCTGAAGCGGCTGCGGAATGCCCGCCGACTGGTTTGGCATACTTGCCGCACAAACACTTGCTGTACGCGCCGCAGTTACGGGTTGACAAGCGGAAAGAGATTGCGTCTTTTCGATGGAACAGCAATTCAGTGCGCCCGGGGGCGGGCAGCGCGAAATGGGCGACGTACATATGGGATATGATCCTCATACCTGTCAGGTTCACTGATTTTGAGCTAACCGACAGCAGAGACGCTGAACGGCCTCGACCGATTTGCGCCAAACGGCAGACGAGATACGCATTGCTGACCGGGGATTCGGTTCGCGTCCCGAATGTATCCGCTCACTTGCTTTTGGGAGAAGCTGATTATATCGTCCGGGTTCACTGGCGAGGATTGCGCTGGTTAACTGCAGAAGGAATGCGCTTTGACATGATGGGTTTTCTGCGCGGGCTGGATTGCGGTAAGAACGGTGAAACCCACTGTAATGATAGGGCAATTCCAGTGTAATAAAAAGCCGAGCTCCCTTTCCGGCACGTCTACATTGCCGTATCACTTCCTCCCGAAAAAGCATTAATCAGTAAAACCCGACTGCTCAGCGAGAATCGTCGAAAAGGACGAGTATTCAGGCGGCAAACGCTGGAAGCAGCGGGCCATGTGCTATTGCTAACATCATTACCGGAAGATGAATATTCAGCAGAGCAAGTGGCTGATTGTTACCGTCTGCGATGGAAATTGAACTGGCTTTTAAGCGGCTCAAAAGTTTGCTGCACCTGGATGCTTTGCGTGCAAAGGAACCTGAACTCGCGAAAGCGTGGATATTTGCTAATCTACTCGCCGCATTTTTTAATTGACGACATAATCCAGCCATCGCTGGATTTCCCCCCCAGAAGTGCCGGATCCGAAAAGAGAAGAATAACTCGTTGTGGAGAATAACAAAAAATGGTCATCTGGAGCTTACAGGTGGCCATTCGTGGGACAGTATCCCTGACAGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCAACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATCACTCCCCGCCGTTGCTCTTACTCGGATTCGTAAGCCGTGAAACAGCAACCTCCGTCTGGCCAGTTCGGATGTGAACCTCACAGAGGTCTTTTCTGTTCCAAGCGCCGCCCATCTACGGCGGTGATACAGATGACGATCAGGGCGAACAAACTCATGCCCTTATAGCTTCATTCTTCCTTCTCCTGACCTTACGGTCAGTAAGATGGCACTCTACATGTGATTCACATAATAGGGAGGCCCTCCGATCT +>contig_3 LN:i:41146 RC:i:12 XC:f:0.819277 +GGCCCGGCCCGAAGATTGCGCGCCACGCCATCAATCAAGCCCCACAGGGTTAAGCGGAGAACTAAGGCAGCAAAACCCGTCAGCATCACACCGCGCCGAGCAGCTGCCGCCATCAACGCTCAACGCCGCCCCAGTCGGTTGGCGTCAGCGTTCCAGTGCTCACAGAAACATGCTTTGCAAGTTTGGTCAATATGTGAAGATAACCAGGAACGGATTGTTGCGCCGCAATAAAGGCTCACTACAACCCGCGAGAATCGCGTTCCCCGGCGGACAGCGTTCCCCCCAGGCGACCGCCCCAAAGACAAATTAAGCCAACAACACAATGCCCCTGTCTGCGCAGCAAACTGGCTCGCCATCGCCACCAGGGACGCCCAGAGCGTAGTGACGGGTAATCCCCAGTTGCGCGCCTGTAGCACGCCAAGGCGCGTGGCTCCGCAGCGGGTTACGCAGCACCTTGCTGAAACGCAGCCCACCAGACCCAGACCCGCGCCCCAACCAGCAGCGAAATCGCCAGATACGCGGCAACAAGCTGTAGTGAAAAATCATCTGCCTCGATTGACGTCAATATCCGGCGACCAGGCGCCTGCGCCCTCTGGCTACGCGGAGCCCTCGCGAGAAGTTCATCCAGGTGAGCGCGTAGCGGACGATTACTAACAGCGCCAGCAATAACGCCGGGAAAAGCGCAATCGATTTACATCACCCGCTTTACCTCCCGATGGCGTTATCCAGAACGCGCACAAAGTGCATTGCCGAGGAGCTTCGCAACCATAAAACCAGCTGCAGGTACGCGCTGAAAGCGTCAGGCGCGGACAAACGGCAGGGCCTCGCCACAGCGGCGTTGCCATTGAGCGCTCTTGTCTTTTGCTGTTGTCGTGATCTAATGCACGAGACTGATCACCGTCCTTTTATACGCCGCCAGAGATCGATTACTGACGGCGGTACTGCCCCAGAAGTTGGTTTCCCCCTTGCCAGGCCATTGGGAGCCGTACTCCAATCAAGAATTTCCTGGGAACAAGGCTCGTTGGATCGAAGACGCAGCATATGGCGCGATCGATAAGGTCGTCAGCAATAACGGACGCGGCACCACGCTATCACAAGCGGGGTTTCATGCTGTAGATAAAGTCTTCATATTGCCGCAAATGCGTTTCCGCTGCCGCTTTGCCAGGTTAAGTAAATCTGCATTTCGTAGCGATTTAGCGCCATCGCCAACGGCTGTTTGGCCGTCAACTTGAAGTGAAATCCGCGACCCCGGCGCAATACCGAGTCAGCATTTCTTGGTAAAGGGCCATATCCTGCCGACCAGGACCAGTAAACCCATGGTTTCATTTTCCGGTCAGCAGTTCAAGGTTAGGTTGCTGTGCCGCAACCGACGTTCGATCAGCTAGTCGCATGGTGGGTCGCTGTACCCACAGGCGATAGTCGATGGGTATCCGCCACGCCGGTAAGGCACGATGCCGAGCGCCAGCAGTAATTCCGACCGGCAACCACTCCCAGCGCCCACAATACGATTGGGATCAATAGCCGGCGCGCGTGTGGCGGGTATTCATTCTGCCAATAACAACGGAGTAAAAGCGCCCATCCGCCCGTTAACAGTCGAGCGGCGCTCGAAATAAGAAGGGTAAGTCCCCGTCTTCATCAATGAAACTCAAAACTTCACTGGTGGCAGCCCACCCGCCGGATGTCGGCAAAATACCCATCTGGGATGCCCATAATCATTTCGAGGGGTTCGCCGCGCATAACTTCCCGCAGGCGTTCCCTGAGCACTCGTTTTCAACCGCCGGCAGGGCGACCAGATAAAATCACAGGCGCGTTGCCGCCACTATTTGATATACGGCACCACGGCAATGGACCGTCAGGCCACGGCTCCTGGACTTTAAAACGGTTGCACCCAGCGACAGCAACATCCAACCTGGTGGGCGGATATCCAGGCGGCCGCGAGGTCGGTCCGTCGAGCAACAGACAACGGCTATCCTGCGCCAACCAGCATGGCGTCCCACGCCCTGCTGACTGTCGCCGCCAGAAGAGGACTATCGCCCAGCCGATGCGCCCAGCGGTTTTTAAAAGGCCAACCAGCGAGATAAGGCTTCCTCGACTTTGTTTCGCGGATCTGCCGCCCGCCAAGCGCGCCCCAGCGCGGCCATGCCACCGGGTAACGACCAAATCGCCACCCGTTCACGCAACGGTCATCCCTTCTGCCGGGAGGAAGCTGCTGCGGCAAAATAAGCCACTCTGCGGGGCAGAACCGCCCTTTGCCTGCTCCACGCTTTCCAGCGGGTTAGGGCAATCGAGAAGGAAGAATCAGCCGCCCTTCCGACGGGCGGCTGATGACGGCCAAGCATTTTGAGCAGAAGTGGATTTAACCAGAAGCCGGTTGGGGGACCAATCAAGACCCGAGTCACTTTCCCGGGCAGGAAAAGGTTTAACCGCACAGGCGGAATGCAATAACGCGTGCGCCCGGGGCACACGAAAGGAGATAAATTTACGCAGTGCAAAAGTGAGTATGGGAATGATTCGTGGTATTCCTGCATTAACAGCCGAACTTATAGAAATGGGCAGCGGAAATCCGGTTGCCCCAGAAAGAGAAATTAGAAACAGGGAAGGTTGCGGTTGCAACGAACCTGGACGTTTCATGCGCCCCAGAAAACGCAGCCATAAGTAGGTTAGAAGGCAGCCTGGCGACGCTTTCACGATCGAAACGGTTGATCTTAACATGCAGCGCCACGTTGGAGCCAGCCATGCCGCGGACTTCGCGGCCAGCATGACATAAACGTACTAACGCATCCACGACCGTAATAACTTCCACTCTAAAGGCAAGTTAGCCCGGGGATCACCCATTAACTGGAATGCCCAGTATACCGACCACCGGTTGCCCAGCGTCAGGACCTGAAGCGGACCGTCAAAGAAGGCTCGGAGTCAGCCCGACAACCCGGCAGCACATGTGCTTTGGCACCTGTGCAGGCGTATTGCCTTTGATAGGTAGTATCGGTTGGTGGTATTCCGCATTCGGTGTAAGTAATAGACCGAACCAGCTACGTTAACACACCTCGCCGACACGCGGGCCGCTTTTCGCTTCGGATTTCTACCGCCTCGTGCGCGGATGTCTCGCCACCTTCAACCGAAGAAGAAGGAACCGCTCAAGGGTCCGCCATCAGGTTGTTGAGTTGGTTTTAGTGAGATTATAACAACGGCCCCCAGGGTAACTTACAATCGGACGATCTTTCCGGATAGAGTATTTCAACGCCGAACTTCATACTGCTTACCTTTTGAGACGGTGCGAAAATTTGATTACCGCGTCCTGTCCCGCAACTTGCGAAGAAGGGTTCAAACGAGATTCGCTATAGCCTGAAGTAAGGTGTTACACCATCTATCAAACCAGGGTACGTTATAACACCACCAACGGCCAGGGTAAACTGTTTGTCATCACCGTTTATCGGGTCGTCCCGGCGAACGCGGGTAAGAGGAATTCTTGTACTTGCCCAGTCATAAACGACCGCCTGGGTGACCAGCACATTTATTCCCAACTGCGCCATGGATCCCGAACACTAAACGCCCCGTTTAGTCTTGCTGGGTTATTATTCAGGCAATGCGGTAAGGGCCGGCAGTATTGCCCGGATCTTTGGCATTGAAGGTCGAAATTCGGTTAATTCCCGGATTGACCAGGATTGAGCAGCGGGCCACTAGATAGTCGTCGTTACCATACCAGGCGGTTGATGTGCATTACGCATACCGCCTAAAGTCGACAGCGGTTCAGCAGTGTGGTGTGGTCGATATTCGCCAGGTGGCAAAACCGTTGCTCTGCAACTGGGTATCAACGGAGAAGCTTTGGGCAGCTTCTCATCCATCGAACCCGGACGGATTTTAACGTTGGCCTGAATAAATGGCCTTTATCCGCTGGCTGCTAATGGCCTGCACACTGTTGGCTGTAAGCATTCGCGCGGATGCGGAGCAGACGCTCGTAGACCATCAAGCGCTGGTTTTTGCGGGGTTTTGTTTTTCAGCAAAGCGCAGGCTTCTGTTACGCACAGTAATACGGTGTACGTTAAAACTCGTAGATCGAAGCTGTTAGCCGACCATCCTTCTCATTACGAGAATAGTGTTTGGTTACTCGCCCCTTCATTAAAGATCTGTCGGCAGACGCTTACCAGATTCGGCCAGCGGCTCAAACGGTTCCCTCTTTCGGCAACCAGCCTGGTAATTACCGGGTTTCTGGCCTCGGTCTGGAGGTTAAGAATAGGGAATGGTTAAAATTGGTTTTATCATCCGGACGACCCAGGTGAACAGCCGTGTGCTATAGCATAACGCCTGGTCTTCCTGACCCCTTTCTGCTGGGCATTTGGCCAGCAACGCCGCAAGACCGGTCAGGCGATAAGAGTTAAACGACCGTCAGCATCCAACGAAATCGCCTAAAGTCAAGACCAGTCTGGAAACTGGCTGTCCAGTACCGGCTTTAAAACTGAACTTTTCTTTCCAGCGGTTCGGTGGTCGGACGGCTATCGGCTGACCCTATTCAACAGGCCCAGGACTGCTTTTACCGTAAACCACGGAACCCCGGGCCACGCATAATTTTCAGCGCGTTCCAGGCCATATACGGGTCAATGACCGCTCGTTATAGAAGTTTGCCCTGCAACTTCAGGCCATTCAGATATTATCTGCTTTGGCCTTCTGCCGCAAAGCCGCGAATGATTCGGGAGGTGTCATAGGTGTGGGATGGCCACGCGTACCAACAGATGACACCCGGCGTGTAGCTTAAGGCGCTTCTTTTCCCGACTTCGGCTGGATGCAGCGCCATCTCTTCGGCGGGTCACAACAGAAATAAGGATCTGGGCACTTTTTGATCGGCGCTATCGGGTTTTATGCCGGTAGCAGACTGTCGCGCCGCAAATAGTTGCAGCAGGTCCCCCCCCTCGCGCTTCTTGCGGGCCAGGTGCACGGTAACGGGTAGTGTCTTCGTGTCGGTTAAACCGCTGCCCCGTGCATAAACAGACGTTGACCGCCTAACCCGCTGTGTCCTCTACAACTGCGATTTTACGCATGAGTGTTTTGGCTGGGGAAGTTTTGGAACGCGTCCATTGGTATCATCTCTGATGTAAAGTGAATGATAGTAAACGAAATGATTATTATAAAGATACGATAATATTTTCGAAACGCTGGGCGGGTTCGCAACGGTACGGCAATTCTGAGCGTCCTGAAGGGTTTATAATACAACCAGATGAAAAGAAGGGTTAATATCTTAGATGGGATGTTATTAGTACCGGGATGGCAAACTTCGCCATCCGGTATTTCAACGCTTAGATGTTAATTACTACCAAACATATCCTGCTCAAGCCGGCTACACCGTCGCTGTCTTTCTGCTTTGCTGAGCAGGTTGCTGGTTGCGGGCTTGTTGCTGCGGCTAGAAGACTTGATCAAACGGATTGCCTGACGGCCTGCTGCTGCATCTCGGCTCTGCTGGCCACAGCGATTGCGGATCGCGGTCCAGAGCCGGCAAGATACGCGCATGCCACCCGCTGCAAACAAAGTTGCCGTCGTAAGTCCACGCCCAATTCTGCAATATCTTCTGCGCGGAACAAGATTCAGCGCGTTGGCGTCTGGTTAGGCCAGATAACGCTGATAAATCGACATTGCCCGGCTGGCACCATACAGTTTGGTCGGCTGGTTGTTATCACGGCCGACGCAGTGATGGTCACCGTGCTGCCGTCATGCCCGCAAACCCAGGTATCTACGTTATTGTTGGTAGTCCCTGTCTGTTCCTGCCAGATGCAGGTTGCGGCTATTTCGCCCCAAGGCTGACGGACCCCGTACCGCGTTGTACCACACTGCTGCATGGTCCCAAAGTGTCAGATACGCCGCCTGCGCCGGAACAGCGCGTTCCGCCTGCGGAAGCTCTGAATACAGCACACTTTGCCATCTTCCGCGATTACCGACGCGCGCAGAAAGCGGTGCACGGTTACCACGCTGGCGATTGGTCTGGGAATGCCTGCGCCACTTCGATTTGGCGTTAAGTTCAGACCCCCCAGCAGCGTTCCGGGACCGGATGCAACTGATCTTTCGGTACCGCCCATTTAATACAGGTCTCCCTAACCCGCAGGCACCCCCCAGCGCCATCCCCCAGATTATACCGTCGGGGACGTTCATCGAACGGTCAACGCATCCACCAGCATCACTCTGCCGCTTTCGCTATAACGACGGTCATCATTCTGCGGTGACCAGACCTTGGCCATCGGTCTACGCCAGCGCGATGCCGCATCCGCAATCCACGTATTCAGACGATAGATTTTCGGCGCGCTTAAGGCGTCAGATAAGTCGCGTGGTTTTCACAGGGGACCAATCGAAAACGAACGCGCCTGCATCGCACGGTTGTAGCCCGCAAACTGCGCTCAGAAACCTCCACCATCGCACGTAACTTCACCACTATAAGCGGTCGACGACCACATCGCAGTTTCAAGATCGCTCAACTTACGCTGTTTTTTCAGTTGCGGAATGCCCTTTCCACGGCCGGCTTTTCCTGCCGCGTCCTGGGCCACCGAGTTACAAAGGTAGTGAAGATCTTCACGCCGGAGAGATCTTTTACCTTATCGCCCAGGTTTTGCCTGGCAGCTCCTGGACGCACCAGTTGCATAAAGGCTGGCTGGAGGAGAGATTCACCCACCCGCGCGGCTGAACCCCCAGCGGACGGGCACCTCAACATGTCATAAGAGTTCTTGATCAATAATCCTGTTGCTGTTGCAGCAGAACGCAGCACCAGATTACGTCCGCTCCAGCGGCCAGTTATTGGGTTACGCCACGCGGTTGTAGGATGGACGGCCCTGTCACCATACCGACTAACAGCGCTGCTGAGTCGAGGCTTAGCCTCTTCTACCGGGCGACCAAAGTAAATACAAGCTTGCCAGCGGGAAGCCCGCGGATTTTCGTTTCCGCGGCCTCTGACCGAGATACACCCTCGTTCATATACAAGTCAAGAATACGGTCCTTGCTGTAACGCGCGTGCCCTGAATCAGCGCCATGTAGCTTCTTCGATTTTTACGCCAGTTAAGAACGCTCGTCTGGAGAGAACGGTTTTTCAACCAGCTGGTTGCGTAGCGTACTCGCCCCTGTACCGTCGTCCGGCGGTCCAGGTTTGCCAGCACCGCACGTCGATTGAGTAGAGACTGATTCCATCAGCTCGGTAAAAATGAGGTCTTGTGTCGCCAAGCAAAGTATCCACCAGCAAATTCCGGGGAAACCACTGCGCGGCACAAACAGCACTCTGCTCACCGTTGGCGGAAGAGGATCATGGTACCAGACCGCGGATCAAGACGGAAGAAACCGAACTGACGGGTGTTCCCATATTGACGATCGTCGCCAGATATCGCCATCAAAGGTCAGACGCGCGCGCACCTGTCCTTCTTTAACTGTCCGGGAAGTCAAACGGACGGCAATCATCTCAATGCTTGTTGGCCTGCACGGTAAAATTCCCAGGACCGGTCATTTTCACACCCTGACGATACTGGGTTCGCCTACCAGCAGCTTCACCAATTCTTCTTGCTGATGTCATGTCCTGGCTACAAGTTGACATTCGGCTCAGTTAAACTGCCCGCAGGCAGCTTGCCAGGACCTTGCCATCAATACGGGCTACGAAATTTTTGATCGAGAAAACGCCGTAAATGGCGATCAGCACGGCAAAAGACGATAGCCAGTTAGCAGTAGCCATAGCCAGCCGCGTTTGCCAGCACGAGGCTTACGCCCTTGCCTTTGCCCTTATCTTTGCGCGGCTCGGTTCCTTCATCTCATATCATCCTAATCGTCGGTAATCACGTCCATCTTCGTACGACGACGGCTTACCTTTTGTTTTGACCGGAGCGTCGGTTTCGCTTTGCGTCCAATTGGCTCGCGGTCATTCCCGGCCATGCGATTATTCTCCGCAATATTAGGCGCAAAAGCCCGATTTTCTGTTCTTCTGTTACAAGACAGAAGAAGATATCTCCAAAAATTTGTGCTCGATCGGTCCCCCTCTCCCTTGAGGGAGAGGGTTAGGGTGAGGGTGAACGTGCGCGCTGATGCCCTCACCCCCGGCCCTCTCCCCACAGGGAGAGGGAGAAACCAACCTTACGAATACTTTTTCGTCCGTCGCGTCGGTGCAGTATTGTCCGGGGTCGTCCGGCCAGACATGTTTGGGATAAACGCCCTTTTCATCTCTTTTTGCACCTCACGTACGCTCCTTTCCAAGAAGTCGCTCAAATCTCGTGTGTATTTGTAATTGGGCCTTTGGGGCAGGTGAAAGCAACTCCAGCACCAGCGGCACGCGCCCCTGGGCGATCGTCGGATTGGTGGCCTCGCCAAACATCTCTTTGCATTCTCACCGCCAGCGCGGGCGGGTTATCTTCATGATAACGAATGGCATCCGGCTTCCCGTCGGCACAGTGTAATGCGCAGGCATTCACTATCCAGACGTTGCTGCATTCCCCAAACAAGTCATCCGCGTAGTCCTGATAAATGTCGAGTGATTTCAGGGGTCGCGTAGTGAATGTACGCCAGTCATATGTGTGGCAGCAGCCACGTTTCCAGCGCGCCAATAAACTTTTCATCATCAACCGCTGGCCAGTCATATTCCGGCAACCACTTTGCGGCGCATAACAAACGCAAGCGTAGCTGTTCCGCTTCGCCCGTCCAGTTGAGCACGCTTAAACCTTTATCACGGATGCCATTAAGCATCGCCTGATTGCAACTCGTCTTCTGACGGTTTCGCCAGCGGCTGCGATTTCACCGTCAACTGACCGATTTGTATGCCGACGCCAGGCCTTTCAGCGTTACCTTGCGCTCACCCACTCCACAGTGTTCAGACTGCTGGACAGCTTGCGGGCAGCGGTGTACTAAATCATGAATATCGACCAGGCAGCGCCATAAAATCCCGCGCATCCCGGCCAGGCGCTGCCCTGAATAATAACGGTGCGATAACCATTCGTGGCGGCTTAGCGCTCGTTGGCATCGAAGCATCCTCCATGCCGATTTTGCAGTTGATAGCGTCCATCTTGCCCACGGCGACGAGCAATGCGATCGGCAAACGCCCCGGCAGTAGCGGCGCGATAAGCGAACTGTCTGCCCACCGCCACGTACGTTTATGCGTTTTAACAGTTGCTGACTACGTTGCTGCCAGGCGGTTGAGTTGCGCGAAAACGCCAACGCCCCAGGTCATTATGCCCACCGTTGGCGCTCGTCGGAGATGGCGGCACTTTTTGCCGCGGTAGACAGCTTCGTCCGTCGTTCTTCGCGCTAACCCAGCATTGCCCGCCTAAACGCGGGATCGTTACCCAGGCATGCTGTCCAATTTTTGCCCTTGCCACTAACCGTTCACCCTCCAGTGCCCCCCAGCATTTGTAACAGTACTTTCGCGGCCAGTAGATTCACTACTGGCGGTTGATCCAGCGAGCCAACTCATCTGCGCGGATCGCTGCACCCCATTTGCATAATTCATCAGCCAACCGGAAAGATACGCTTTGTAAGATTCTTCGGTTCACTTTGCGCCCGGGCGCGTTCTGCTTGTTTCTTTTGGCGATAAATGCCAGCCTGATACCCGGCTCCAGACGCCCGGCGGCCCGGCACGCTGCGTCATGGATGCCGGCTAACGCGTTGAGTAATCAGTCGCGTAAGCCCCGTCGCGGATCAAAACGGCCACACGCTCCTGGGCACAATCCACCACCAGACGAATACCCTTCAATGGTTAAACTGGTTTCAGCAAATTGGTCGCCAGCACCACTTTGCGCATCCCTTGGGTGCGCGGAGGATCGCTTTTCGCTGATCGTTCAGCGACAACGCGCCATACAGCGGGCAGAGACAATTACATCACTGCCGATGCGCGAAGCAGTTGTTCCTGCACACGCTGAATTCTCCGACGCCAGGTAAAAATAACAGTAATGATCCGCTTTCCTGACGCAGCATTTACAGCGGTGGCTACCGCAACGGCACTCGTCAAAAACGCTGATGCGCGGGCAGCGGTAAATAACGGCGTTTCAACCGGAAACGAGCGCCCTTCTGAGATGACGACAGGCGCTTCTGGCAGCATTTGCTGCAAGCGGTCGTTGTCCAGGGTAGCCGACATAATCAGCAGTTTAAGGTCATCACGCAGACCTGTTGCACATCGAGTAACAGCGCCAACGCCAAATCCGCCTGCAAGCTGCGCTCATGAAATTCATCAAGGGATCACCAGTCCAACACCGCTCAGTTCCGGGTCACGCTGGATCATGCGCGTCAGCACGCCTTCGGTAACTCATTCCAGGCGGGTATTCGGCCCGACGCAGTTTTGCGCACGCATCCGTAGCCAACGGTATCGCCTGGCTTTTCGTTAAGCAGCTGCCGCCACCGTTTGCGCGACGTTACGCGCCGCCAGACGACGCGGCTCCAGCAGGATAATTTTTCCCGTTAATGCCGGGATTGCGCCAGCAGTTGCAGCGCAGCAGGTTGATTTCCCGGCCCCGGTCGGCGCACTTAATAATACCTGGGCGCACAATCGAGAGCGGTAAGTAATTCAGGTAAGACGGCAGCAACGGGCAACGACGACACAAAACGCTCCAGAGGGTTAACTATTCTTCCGCCACATTGTAGCCATCGCGGTAATTCATAACCGAGTGCCTCACATGTCTGAACCGAACGTCTGTCTTTGCTATCGACTTACCTGCAAGAATCCGCGAACAGATTATCCATTGGCGCGCCACACACTTCCCACCCCTGAGGCGGGACGTCCGGTCGCGCCGATAATTTGCATCTGACTGCTGGCATTTTTAGGCGAAGTGAGCGCCGAGAAAGAGAAGGCGCTTTCTCTTTAGCCGGACGGATTCGTCAACCTGGTTTTCACACTCACGCTTGATGACGCCGGACAATGGCTGCGTTCGCGTGTGGTGTGGTTAGGATGCGTCAGCCGCCACGCGGCTTAATCCAGCTGGCGAATATGCTCCGTTCACAGGCTGAGCCCGCAGCGGTTGTTTTCAAAGCAATCGTCCCGTTTCATCCACATATTACCCTTATTGGCGCGAGCAGACGAGGCGGTGACAATCCCGCGCCAAGGTTTTAACCTGGTCGTATGCGGTGACGGGTTCACCCTTTACGCTCCTCGTTTGCCCGTGGACGCACACGCTACACGCCGCTAAAACGCTGGGCGCTAACGCAATAACAAGGATTGTTCGCAATGGAATTTTTTCTCCCCCTCTACAGCGCGCGACGCTAATTCAGCGTTACAAACGTTTTTTAGCCGATGTGATCACACCCGATGGTCGCGAATAACGCTACACTGCCCGAATACGGGGTGCGATGACCGGGTTGTGCTAACCGCCTGGCCGATACCGTCTGGTATTCGACTTCAGACAACACCAAACGGAAAATACCCACACACCTGGGAATTAACCAAAGCCAGAGCGGCGCATTTATTTGCGTCACAACGCTTTTGGGCTAACAGGTTGACGACAAGAAGGCTATCCTTAAATGAATCAATTTCAGAACTGTCAGGCTATAGCTCGCTGAAAAGCGAAGTAAAATAACGGCCAGAACGCAGCCGTATTACTTTATGTTGCAGGCGGATTCGCGTCCAGACTGCTATATGAAGGTGAAATCCGGTTACGTTAGCGGAGAACGAACAGGGGATATTTTCCCGATGCGGTCACTGAACGAGGGTCAGAAACACCTTCGGGAGTTGATGAGCGTAGCGGCTGAAGGCCCAGCGTGCGGTTATTCTTTTTCGCCCGTGCTGCATTCAGCCATTACACGGTTTTCACCCGCGCGCCACATCGATGAGAAATACGCGCAACTATTGTCAGAGCTCAACAGAGGGGGGTAGAAATTCTGGCTTTACAAAGCGGAAATTTCTGCTGAAGGCATGGCTCTTTAAAAAATCACTGCCGGTTACATTGTAGTAAAGTAAGTAACTGGTTAATTTACACTCTGGTCGCGTGGCAATACGCTTTTCCTCACACAGTTGTCAAAGTGTTACGTTTAGTATAATTGCTATCCCGGAAAAGCATCTGCTATTTATAGCGGCCTCATTTTTTCCCCGAACATGGGATCCGATAGTGCGTGTTAAGGAGAAGCAACATGCAAGAAGGGCAAAACCGTAAAACATCGTCCTGAGTATTCTCGGCCATCGTGGGGTGGAACCATATCTGGAAAGCCGGGCGAAGAGTATATGAATGAAGCCCAGCTGGCGCCACTTTCCGTCGTATCTGGAAGCATGGCGTAATCAACTCAGGGGATGAAGTCGATCGCACCGTTACACATATGCAGGATGAAGCAGCCAACTTCCCGGACCCGGTAGACCGTGCAGCCCAGGAGAAGAGTTCAGCCTCGAACTGCGTAACCGCGATCGCGAGCGTAAGCTGATCAAAAAGATCGAGAAGACCGCTGAAAAAAGTGGAAGACGAAGATTTCGGCTTCTGCGAATCCTGCGGTGTTGAAATTGGTATTCGCCGTCTGGAAGCGCGCCCGACAGCCGATCTGTGCATCGACTGCAAAACGCTGGCTGAAATTCGCGAAAAACAGATGGCTGGCTTAATTACAGCCGTTCCATCACGTTTACCCAGGCGGGAAATTGCTCCCGCCTTATTTTTTGTTTCAAAGAGATGACAGACACACAGTATATTGGCCGCTTCGCCCCCTCTCCTTCCGGCGAGCTTCATTTTGGCTCTCTGATCGCCGCGCTCGGCAGCTATTTGCAGGCTCGCGCCCGGCAAGGTCGCTGGCTGAGTACGCATAGAAGATATCGACCCCGCCTCGTGAAATTCCCGGTGCCGCAGAAAACTATCCTGCGCCCAGCTGGAACATTACGGGTCTGCACTGTGGACGGGATGTTCTCTGGCAATCGCAACGTCACGACGCCTATCGTGAAAGCACTCGCCTGGTTACATGAACAAGGACTAAGTTATTACTGCACCTGTACGCGTGCGCGTATTCAAAGCATTGGCGGTATTTACGACGGTCATTGCGGGTGTTGCATCATGGACCAGACAAGCCGCAGTGCGTATTCCGCCAGCAGCATCCGGTCACGCAATTTACTGACCAGCTGCGCGGCATTATTCACGCCGACGAAAAACTGGCACGGGAAGATTTTATCATTCATCGCCGTGATGGGGTTGTTCGCCTACAACCTGGCTGTTGTGGTTGATGATCATTTCCAGGGCGTTCAGAAATAGTGCGTGGGGCTGATCTGATTGAACCAACCAGTAAGGCAAATCTCGCTGTACCAGCTTTTTGGCTGGAAAGTGCCAGATTACATTCATCTGCGCTGGCGCTTAATCCACAAGGCGCTAAACTTTCCAAAGCAGAAATCATGCGCCTGCGTTGCCGAAAGGCGATCCACGCCCGGTACTAATCGCGGCACTTCAATTTCTGGGGCAGCAGGCAGAAGCACACTGGCAGGATTTCAGCTCGAGCAAATCCCTTCAGTCAGCCGTCAAAAACTGGCGGCTTACCGCCGTGCCCGAGTCGGCAATTGTAAATTCAAACATTCTCAAATGCGTACATGCTGAGCTATGATTAGCCGCTATTTTTTTTGTCCTGAATGATGTTTGACACTACCGAGGTGTACTATTTTTACCCCGAGTCGCTAATTTTTGCCGCAAGGTGCTAAGCCGCGAGGAAAGCGAGGCTGAACAGGCAGTCCGCCCGTCCACAGGTGACGGTGATCCGCGTGAGCAGCATGCTATTTCCCGCAAAGATATCAGTGAAAATGCCCTGAAGGTAATTACAGGCTCATATAAAGCGGGATACCGAAGCCTGGCTGGTTGGCGGCGGCGTGCGCGACCTGTTACTTGGCAAAAAGCCGAAAGATTTTGACGTAACCACTAACGCCACGCCTGAGCAGGTGCGCAAACTGTTCCGTACTGCCGCCTGGTGGGTCGCCGTTTCCGTCTGGCTCATGGTAATTTTGGCCCGGAGATTATCGAAGTTGCGACCCTCCGTGGACACCACGAAGGTAACGTCAGCGACCGCACGACCTCCCAACGCGGGCAAAACGGCATGTTGCTGCGCGACAACATTTTCGGCTCCATCGAAGAAGACGCGCCCAGGCGCCGCGATTTCACTATCAACAGCCTGTATTACAGCGTAGCGGATTTTTACCGTCCGTGATTACGTTGGCGGCATGAAGGATCTGAAGGACGGCGTTATCCGTCTGGATTGGGTAAACCCGGAAACGCGCTACCGTGAAGATCCGGTACGTATGCTGCGCGCGGTACGTTTTTGCCGCCAAATTGGTATGCGCATCAGCCCGGAAACCGCAGAACCGATCCCTCGCCTCGCTACCCTGCTGAACGATATCCCACCGGCACGCCTGTTTGAAGAATCGCTTAAACTGCTACAAGCGGGCTACGGTTACGAAACCCTATAAGTGTTGTGTGAATATCATCTGTTCCAGCCGCTGTTCCCCGACCATACCCGCTACTTCACGGAAAATGGCGACAGCCCGATGGAGCGGATCATTGCAACAGGTGCTGAAGAATACGATACGCGTATCCATAACGATATGCGCGTGAACCCGGCGTTCCTGTTTGCCGCCATGGTTCTGGTACCCACGGCTGGAGACGGCACAGGAAGATCGCCCAGGAAAAAGCGGGCCTGACCTATCACGACGCTTTGCGCTGGCGATGAAACGACGTGCTGGACGAAGCCTGCCGTTCACTGGCAATCCCGAAACGTCTGACGACATTAACCCGCGATATCTGGCAGTTGCGTTGCGTATGTCCCCGTCGTCAGGGTAAACGCGCATGGAAACTGCTGCGAGCATCCTAATTCCGTGCGGCTTATGACCTGTTGGCCTTGCGAGCTGAAGTTGAGCGTAACGCTGAACTGCAGGCGTCTGGTGAAATGGTGGGGTGAGTCCAGGTTTCCCGCGCCACCAGACCAAAAAAGGGATGCTCAACGAGCTGGATGAAGAACCGTCACCGCGCGTCGTACTCGGCGTCCACGCAAACGCGACCACGTCGTGAGGGTACGCATGACAGTGCGGGTAAATTGCCATAGGCAGCAATCTGGCCTCTCCCGGCTGGAGCAGATCAATGCTGCCCTGAAAGCATTAGGCGATATCCCTGAAAGCCACATTCTACCGTTTCTTCATTTACCGCACCCCCACCCGCCTGGGGCGCAAGATCAACCCATTACTTAACGCAGCGTTGGCCGCTGGAAACCTCTCTTTGCAACCTGAAGAGCTACTCAATCACAACAGCGTATTGAATTGCAGCAAAGGTGCGTCCGCAAAGCTGAACGCTGGGGACCACGCACGCTGGAACTCGACAATCATGCTGCTTTGGTAATGAAGTGATAATACTGAACGCCTGACCGTTTCCGCACTACGATATGAAGAATCGTTGATTTATGCTGTGGCTCGGCTGTTTGAAATCGCGCCGGGTGTTGTTTCCTGTAATGGGGAGGATGTTGCTCAAATCTTACATACAAGAGCATTTGACAAAATAAATCCAAATGCGTAATTTATTTACCGTATTGCGAATATAAATATATTTCTGAAAATATATCTCATCTCTTTTCCTTAATTACGATTTTAGTTTAAACATCTCTTTGCGGAAATAATATAACTTCCTATAATAAATATCAATCCCCATCTCCATACCCTAACCAATTGCACTTGTGAACTTTTTTTACTTAATTTTGAATTAAGTAAATTTAATTTGTTCAAAAGCTTCTAAAGTGCACAATAGGCGACCTAATGAACAATGGTTGTAAATTTGGGTAACAAAATATCATTGCTCATTAATATCTAACATTTGTTTCTCCTATTTGTGTCATGAATAGTCATTCTTCAAACATGTAGTCTGACTAATGGAATTTTATAAAGGATTTTTATGTCTAAAAACAATTAGGTTTTGCCCTGTCTGGTCTTATGCTGGCGATGGTTGCAGGTACTGCTTCGCTGATATGGACGGCGGTCAGTTAAATATCAGTGGTCTGGTTGTTGATACACCTGTGAAACCCGCGTTGACGGCGGTAACAAGGATGGTCTGATCCTGCTGCAAACCGCAACCGTTGGTGAAATCGATGCTGGCGTACTGAATGACACCGTTGGCGCTAAAGCTGAAAACCTTTCAGCATCACCGTTGACTGCAGCAAAGCAAATCCGAATCCAGGTAGCACGGCTAAAATGACCTTCGGTTCTGTTTTCTTTGGTAACAGCAAAGGGACTTTGAACAACGACATGTCTATTTAACAACCCCATCTGACGGCGTTAATATCGCTCTACACAATATTGATGGTTCCACTATCAAACAGGTTCAAATTAACAACCCTGGCGATGTGTAGTACTAAAGCCCTGGATTGCAACGACAAAATCTGCTGTTTATGATTTTAAAGCGTCTTACGTTCGTGCCGTCGCAGACCAAACAGCAACTGCTGGTTATGTAAAAACTAACACTGCATACACCATTACTTATCAGTAAGTATTCACCAGTAAATTGATTGAATGAATATACAGGGAATAATAATTTCTATTTATATTATTCCCCTGTTTTAATTAAACTCTATCAGGGATGGTTTATGTTTTTTAACACCAAACATACAACAGCGCTTTATGCATTCGTAAACCGTGTATGGCTTTTAGTTCATCGTCTATTGCGGACATTGTCATTTCGGGTACTCGCGTAATAATATAAAAGCGATCAAAAAAAGTTGTCACAACGTACGTCTGGAAAAAAAGGGAATAAACCCGTTGCTTGTCCAGAGTTGGTTAGATACTGGCGATGACAAAAACGCTGAGCCTGGCAGTATTTACAGTCCCTTTACTGCTACGGCCCGCCAGTATCGCGTATTGAGCCAAACGTGGGCAAACAATCAAATTAAGTACACAGGCCAGCCCCTCACTGCCTAAAGACAGAGAGAGCGTGTTCTGGTTTAACGTACTGGAAGTTCCACCAAAAACCAGATGCAGAAAAGGTCGCGAATCAAAAGCCTGCTGCAACTGGCATTTCGCACACGTATAAAACTTTTCTATCCCCGGATGGATTGAAGGGGAAATCCCTCTGAAGCCCCGTTAGCCCTGAAAGTGGTTCTGGTCTAGGTTCAGAAGGTAAGGCGTCATTTACGAGTGACCAATCCAACCCCTTACTACGTCTCTTTTAGCAGTGGTGATTTAGAAAAGCTAGCGGTAAACGCTATCCGATTGATGTGAAAAATGATTGCACCATTTAGTGATGAGGTCATGAAAAGTAATGGCCTTAATGGCCGAGCGAATTCTGCAAAAGTGCAATTTTTACGCCATTAATGACTTTGGTGGCGCAATTGAAGGTAATGCCAGGCTGTAATCAGGCAAGGATATAATTCCGCAGGAAGCATAGCGTGACTATAGAATATACTAAAAATTATCATCATCTGACCCGTATCGCCACGTTTTTGCGCGCTGCTGTATTGCAATACTGCTTTCAGTGCTGAACTCGTTGAATATGACCATACCTTCCTGATGGGGCAGAATGCATCTTAATATTGATCTCAGCCCGGTACAGTGAAGGTAACCCCGCCTATACCGGGGTTTACGACGTCATGTTTAGTTAAACACCAAGCCAATCATTAACCAAAATATTACATTTTTCCCAATTTGAAGGAAAAAAAGAACGCCAGGCCTTGTATCACATTAAAAGATTTTATGCAGTTTCATATAATTCTCCGATATAAATAACAAAAGCCGTTCTGCTTGCCAGGGATGGAAACGCTCGGCAATTGCCTCAATTTGACGGAAATCTATCCCCTCAGGCTTCTGTTCTTATGACGTTAACGATTCAACGTCTGGATATAGACGTTCCTCAAGCCTGGGTAATGAAAAATTTACCAAAAATATGTTGGTCCATCGTTATGGGAAAACGTCATTAAAAATGCGGCCATGTGTCATACAACCTCAACGGATATCATAGTGAAACCCCTGGTCGCAAAAATGAAAGCCATTTATGCTGCATTTAACGGTGGGATGAATTTAGGTGCATGGGCGATCTGCGTGCCTCGGGCAACTACAACTGGATACCGTTCTGGCAGTAATTATAGTTTTAAGAACGGTATGTTCAGCGTGAATCGCCTCGGCTGCGTTCTCAACTCATTCTTGGTGAGTCTTATACGACGGGCGAAACCTTTGATTCCCCCGTCAGTATCCGAGGCATTCGTTTATACAGTGACAGCCGCATGTTGCCCTCCAGACTTTAGCAGCTTTGCGCCTATCATTCATGGCGTTGCCAATACCAACGCCAAAGTCACTATTAACGCAAGGTGGCTATAAGATTTATGAAACGACGGTGCCGCCAGGCGCTTTCGTCATTGATGATCTGAGTCCGTCAGGGTACGGCAGCGATCTTATTGTTACCATCGAAAAGAATCCGATGGCCAAAGCGGACATTCTCGCAACCTTTCTCATCCGTTGTTCCAAACGGTTACGCCGGCGTTGGGACGTTGGGATATTAGCGGCGGTCAGGTCTTAAAAGATGAATTCAGGATAGCCTATAATTTATTTCAAGCAAGCTACTACTACGGCCTGATAACTATCTGACGGGTTATCACCGGTATTCAGATCACGATAATAATATACCGCTGGTTTGTTAGGTCTTGGCTGAATAGCTTAGTTGGTGCATTTTTCTTTCGATGTGATCATTCCAATGTTCGTATCCGGATGATAATAACATACCAGGGGCAAATTATCGTGTTTCCTGGAACAAGTTATTCGAAGAAACAATACTTCACTGAATATCGCGAGCCTATCGCTATTCGAACACGAATTACCTTGGTCTTAATGATGCACTAACTCTAATTGATGAAAAGTGAACATCCCGAACAAGACCTTGAACCGAAATCCATGCGAATTACTCTACGCATGAAAATCAGGTTACGGTCGAGTATTAACCAACGTTGAAAGATTTGAGAAAAAAGATTACGGTTATTTTATCTTTCCCGGAAGTTGGTCCGATTACTGGGGCTTCCGGACAAATCGTAGCAATTACTCTATTGGCTACAGTAAAGTACATCCGGGGCAGCTACAGTGTCAGGGCCCAGCCCGTTCATGGAATGAAGACGGCGACACTGAACGATAGCGTTTATCTCTAGTTTCCACCATTCCAATTGAAAAATTACTTGGCACTGAACAACGTACTTTCAGTTTCCAGAGTATTGATACCTCAAATAAGCAGTGACTTTAAGGGTAATAACCAACTCAACGTTAGCAGCAGTGGCTATAGCGATAACGCTCGCGTCAGTTATAGCGTGAATACTGGCTATCGATGAATAAAGCCAGCAAAGATTGAGTTATGTTGGGGGTTATGCCAGCTATGAGTCACCATGGGGGAACGCTGGCAGGTTACAATTTCTGCAAATAGCGATAACAGCCGTCAAGTTTCTCTCAGCACCGACGGTGGTTTTGTATTGCAATAGCGGTGGACTGACTTTCAGTAATGATAGTTTTAGCGACTCCGATACACTGGCGTAGTTCAGGCTCCAGGTGCTCAAGGAGCGCGAATAAATTATGGCAACAGTACTATCGATCGATGGGGTTATGGTGTTCACCACGTCTCTATCTCCTTATCATGAAAAACCGTATACCGCGTGGAATCAACGATCTTGAGAACGATGTTGAATTAAAAAATGTACCAGTGGCAGTAGCTGTCCGCGTCAGGGGTTCAGTATTTTGCTGATTTTGACACCCGTGCCAAGGGCAATCAAGCCATTATGAACATCACACGAAGTGATGGTAAAATATTCCATTTGCTGCAGATATTTATGATGAGCCAGGGCAATGTCATTGGTAATGTGGACAGGGGGGACAAGCATTTGTTCGTGGTATTGAGCAGCAGGGGAATATCAGCCATTAAATGGCTCGAACAAAGTTAAACCGTAAGTTGTGTTGCGCATTATCAACAAAGCCCCAGAAGAGAGAAAAATAGCACAATTATTATTCCTGAATGGAATTCGGTGTCAGATTCAGTAACTACAAGGGACGTCAAATGATAAAAACAACGCCACATAAAATAGTGATACTGATGGGAATTTATTATACCCTCAGTATTTGCAACGGATATTAATGTAGAGTTTACAGCCACTGTCAAAGCGACAACCTGTAACATCACACTTACTGTAAAACGTCACGCAATGATGGCAATAATAACTTACACATTGAGATCCCTAAGATGGGTCTGGATAAGATCGCGAATAAAACGACAGGAATCTCAGGCTGATTTTTAAACTGGTTGCCTGTGGGTGCAGAGTGGCATCATACGTTGGATTGATACCACTCTGACCCGGAAATGCATCATCAAGCTCACCTAAGCTTATTATCCGCAGTCTGGTGATTCATCTTCGACGACAAGTAATATCGGTAGGGTTTCAAAAACGGACTACTGATGATGCCACTTTCCTTAAACCTAAACAGTGCGGAAAAGATACGCTGGACACAGACGAGATGCAGCCCGATAAGGGTCTTGAAATGACCGTTGCGCTACGTGAAACAGATGCAGGGCAAGGCGTACCGGGGAATTTTCGTGCACTGGCGACGTTTAATTTCATCTATCAATAAAGAAGGATAATTATGATGACATTTAAAAATTTTACGTTATGGATTATCCAGCAGTGTCGTTTTGGCTGCCTCATTGTTCAGCGGTACTCTCTTATGCGGCAACAGACAGTATTGGACTGACCGTTATTACTACTGTAGAAATGGGTACTTGTACCGCTACATTAGTAAATGACTCTGATCAGGACATTTCTGTTGTTGATTTTGGTGATGTATATATTTCTGAAATCAATGCCAAGACCAAAGTAAAAACATTCAAACTCAAATTCAAGACTGTGCGGGGTATCCCCCATAAAAAAGCGCAAATAAAATTAACCAAGCGAGCCACATGCGAGGGGAACTGCTAATGACGGTGCGGGGGTTTGCAAATGGTTCCACAGCCGCAGATAAAGCAGTGCTGTCGCGCGTTGAAGTCTGGAGCACTGTAACTCGGCAACAGGGAGTGCAACACAATTTAGCTGTGTAACACCAGCATCACAAGAGGTAACAATCTCCACTGCAGCCAATGCGGTCGTTTTTATCCGATGAGTGCACCGCCTGGTCGTGGAAAAAAATAAAACCGTAAACAATGTCACTGCGGGGTAAGTTTTCTGCACCAGCCACATTTACAGTAACCTATAACTAACAGACTTACAGATTCAGGAACTATATATGCATCCCACTCAACGTAAGCTGATGAAGAGAATAATTCTGTTTCTGTCATTACTGTTTTGCATCGCCTGTCCAGCCATTGCTGGACAGGATATTGACCTGTTGCCAATGTAAAAAACAGCACCTGCAAAAGCGGAATCAGTAACCAGGGTAAATATTGATCTTGGCGGTCGTTGGGGTGGGATATTTTTCAGGTAATCGGTTACTCCTGAAAGTTATCAACCAGGGTGGAAAAGAGTTCACTTATCACTGTATCCGACTGTGCATTACAGGGAACTGGCGATGTGCTAAATCAGTTACATATTGATTTTAGAGCCCTTAGCGTGTCATGGCTGCTGGCTCTAGGCTAAATATTTGCTAATGAAATTTTCGTCAGGAGCAAGTAAATGTAGGAGGTAGTTATAATTTTCTACTCAGGATTCGGCGAATACTCAATGTTCTTAATGCTTCAGGCGGATCTCGTTCCGTTTATCCAGTAATGTCGGATTGACATGAATGGTTCATCCTGGAAAATTTAGCACCCGAATGCAAAAAATCGATCCTGCATTGGAAGTGTACATCTGGGTCAACTTATGAGCCATGTGTCTAGTGGATATTTACTACGAATAACAAAAAAAACAGCGCAAACCATAACAATAAAGAAACATTGGAGTAAAAAAATGAAAGACTATCTTCAGGTACATTCTTTTTTAGCCTGTATTCTTGTTGTAATAGCCAGTCAGTGCATATACAAGTTTTATTGTGGGAAATAATGCAGGAGTTGATAACTATCGAGGCCCCTCCACTGCCGCACAGATGACCTTTAATTACACATCAACAGCAAGCAACTTGGTTTTTTATAAAACCCACGCAGCTCGGGCCCGACTGGGGTAAAATGTACTGGTCATACCTGGATACAGGTACCGTGGTGGTATTCTTTACTGCAATACATCTGGCAGAGCGAATCCTTGGTCCAATAACTATTGAAATGCCATGGTCTATCAGGTAAGATTATGGCGGACATTAAACTATTTATACATCTGTTCCTGGTCTGTATTACACCATGTTAATATCAAGGGTCTGGTCTGATACGATCAATAACTGACATTCAATCGCCAGGGAATCTATATCGGAGATCCCTTCCAACCAAGAATTTTTCTTTTCCGTCACAGACAGCGATCTACAAACTAAGGGTTGCAACAAAGCAGACGACTACGATAAGTTTTGGGCTATTGGGTGGTATAGTACACAACATAACTGTTGAATTTTCATACAGATACTAATTTCGATCCTACACTTAATCAGCAAGTCCAGTTATCCAGTTATCAAATTACCTTATTCGTTTAAAGGCCTACAGTCCTGGTACAAAAGTTGTAGATCACAGCAACCACATTTATGTCACATTTCACACTGAATAATGTCAAATTAACCTTTACCAACATGTTTTACCTCCATACTTACCGGGCCATCAGTCAATGGTTCAACGGTTTAAATGGGAGAATATAGCTCTGGGACAATTAAAAATGGCGCTTCACCTGTTCCCTTTGATATCTCGCTTCAGAATTGTATTCGTGTGCGTAATATTGAAACAAAACTCGTCACTGGAAAAGTAGGTACTCAAAAACACACAACTGCTTGGTAATACGCTTACTGGAAGCAACTGCGCCAAAAGGCGTTGGCGTACTCATTGAAGGTTTAGCAACCAGTAAAAATCCGCTAATGACATTAAAACCTAATGATACGAATTCTGTTTATATAGATTATGAAACCGAAGATGCAACCTCCGATGGGGTTTACCCTAATCAAGGTAACGGGCACATCACAGCCTCTTCATTTCCAGGCAACATTAAAGCAAGACGGGAATATTGCTATCGAACCCGGCGAATTTAAAGCCACCAGTACTTTCCAGGTAACCTATCCCCTGAATAACGTAGCAGCACTGTCCACCTTCTGGCAGTGGCGCTGCTACGGATCAAATCATTGCCCCCACCAATGTGACTGATAAAATGCCAGACAGCACAACATCAATTTATCAGGGATACGTTATGAAACCGAACCCACCAATCTCCTTACTGCAGACGTACAAACAGGAAAAAAAACGTTTCCGACCATCATCCGCTTATGACTATAGCTTCGCCAAACTCTTTGCTGATGAAGGGCTTTAACGTCATGCTGGTGGGGCGATTCGCTGGGCATGACGGTTCAGGGGCAGCGACTCCACCCTGCCCAGTTACCGTGCGGATATCGCCTACCACACTGCCGCATGTACGTCGCGGCCCGCCCCAAACTGCCTGCTGCTATGGCTGAACCTGCCGTTTATGGCGTTATGCCACGCCGGAAACAAGCCTTCGAAAACGCCGCAACTGGTTTGCGTGCCGGTGCTAAAATGGTCAAAATTGAAAGGCCGGTGAGTGGCTGGTAGAAACCGTACAACTGGCTGACCCGAACGTGCGTTCCTTATGTGGTCACTTAGTTAAACACCACAGTCAGTGAATTTTTCGGTGCTACAAAGTTGCAGGGGCGCGGGCGATGAAGCGGGCATCAACGGCTCAGCGATGATTAGCCTTAGAAGCTGCTGGGGCACAGCTCTGGTGCTGGATGCTTGCCGTTGAACTGGCAAAGACGTATTGCCGGAAGCGACTATTGGCGATCCCCGGGTTATTGGCATTGGGCAGGCAACGTCAACGACGGCAGATCTGCGTGATGCACGACGCCCTTTGGTATTACCGGCGGTCACATTCCTAAATTCGCTAAAAAATTTCCCGCCGAAACGGGCGCATCCGCGCGGCTGTGCGGCAGTATATGGCTGAAGTGGTGTCCGGCGTTATCCGGGCGAAGAACACATTTTCATTAGGAGTCACGTTGTGTTAATTTATCGAAACCTCCTGCCGCTGCTGCGTCAAGCAAATTCGCCGCCTGCGTATGGAAAGGCAGCGTGTGGCGCGGTGCCTACCAATGGGTAACCTAGCACGATGTGCCATATGAAGCTGGTTCGACGAGCCAAAAGCCGCACCGATGTGGTCGTTCGCAGTATTTTCGTTAACCCATGCAGTTCGACCGCCCGGAAGATCTGGCTCGTTATCCTACGGACCTTGCAGAGGACTGGCGAGAAGCTAAACAAAGGTAAGTGGATTTAGTTTTCGCCCCTTCGTAAAAGAGATCTACCCGAAGGTACTGAAACCATCCTTCGTTGACGTTCGTGGCCTTCGACCATGCTGGAAGGTGGCCAGCCGTCCGGGACATTTTTCGCGGCGTCTCGACTATGTCAGCAAGCTGTTCAACCTGGTCCAGCCGGACATCGCCTGCTTTCGGTGAAAAGATTTTCGCACCTGCCTGATCCGCAAAATGGTTGCCGATTAGGGGCTTCGAATTATTGAGATGGCGCGTGGTGCCAAATTTATGCGCGCCAAAAGACGGTCTGGCGCTAAGTTTCCCGTAACGGTTATCTGACGGCGGAACAACGCCAAATTGCCCTGGTCTGTACCAAAGTTTTTAAGTCGATGGGCGACAAATTGCAGGCTGGGAACGGGATCTTCATGAATTATTAACCATTGCGGGGTCAAGAACTGAAAGAAAAAGGCTTCCGCGCCGATGATATTCAGATTCGCGTGCTGCGACACATTGTGGAGTTTTCTGAAACCAGCAAACGGGCAGTAATTCTGTAGCGCCTTGGCTTGGCATGATGCTCGCCTGAGATCGACAACAAAATGGTCAGCTGCCGTAATACTTAACTGGCGCTACGGCTGATGGCGCCAGTTATTAATTTACCCCACGTCAGCTGTTCAAAACACAGGTTAACTTAAATAAAGGTTTAATTACCTATCAATCTCAGCAAGAGGTAAACTTTGGCATCTCTGCAACGTCTTTCCCGAGACATCCTTTATCAGAAGACGCTGGGGAATTCCTGACTTACTTCCTGTCTTCCCTGCTGAAATCCCCTTTCAATCCCTTTCTCTTCAAACCACTGCGCCAGCGCATCATAGACTCCCCTCCCGTTTCCCATGTCTCTCACACACATCCGTAAAAACAAATCCGCTTGTTCAGTATGACCGCGTTTGCGCATATAGTTTGCATGGCAACCTAAACGACCTTCCGCTAGTGTAGCCCTTCGTCGATCAGCGTGACCATTGCCTCAAGCAATAACATTAAGTCGCGCTGGCGAATATGTTTTTGCAGTAGTTCGAGTATCGCAACCGCCGATGTTGCAATGATTTCGGTACACTCCCGGGTGTTGATGGGGATATCCACCAGCGGGAAGGACTGTTATTAGAACGGTCGCGCCAGCTCCGGCGAGTAAAACATATCAAGCCAGCCACATTGATTAGCGGATAGGTGTGGCCTCGCCCCTGATAAAACAGTATCGGCACCACCAAGCGGCAGCTTATCGGTGGTCAAGCCTCCAGATGCCTGTGCATGGGGCTATTAGATAACGCATCATGCGAAAAGCATTTTTCTTATCCGGCTTGCCTTTGGTGTTCAATCCTACAAATGCAGATAACCGGGATTGCCCTGCATTTGCACGGAATAGAGCCCACGTCGTGCTGTGTCCTTTCAGGGCTCTCTTCAATGAAACTCCCCGACTCTAATGAAGCGGTGTTGAGGTACAAAAGTTCGGCGTAATCCCTGGCAAAATGTATCTCCAAAAGTCGCGAGCCGTCTCCGCATGCATTAAAAATTGTTTAAATAACCGCGTCATGCGGGTTGGTACTCGGGCACCATCTTCCCCTGTCTCCGTGTAGCTTTGCGTGAAGGCAGTATCCAGGTCAAGCGCGCGCTATCGCAGCCACCTCTTTTATTGTTTACCGAGCAGCTTCGAGAAATATCAATTCGTACAGGCGGTACATGGCACGCTTCGGTGCGGTACGCAAACTGCTGACCGCAAATGCCGTCAAGATGGAGTAGACACTAAAACAAAAATCGGGCAATGACTGCGTGAGAATTTCCTCCAAAGCAGGCATCGCCTCTGCGTCATACGACAGGTAGTAAGTAGAAAGTTTTGATTCGCACGATGCTGCGGGCAAAACTCCCACCCGCGGTGAAAGTGCTCATGCGGACACTCATATGAAGGTTCTTGCGCCATTGACCAGGATTTTCTTGACGCTGCCGTATTCTCGAAAACGAAGCCATTGATATCTTGGAATGTCACCAACGGCAAGCGTTTCTCCACTTTATCCTCATGCGGCAGAAGCGGTCGAGAATTTTATTTTGTTAAAACGGTGCGGCGGCCCACTGCGCCAGTGTCGGCGAATATTTGTCACCATCGCAGCTTCGTTACCAGCCAGATGAAGGAAGCTCGCACCTGGCGACCCAACGTCGCCTATTTTGAAGGCGACAATGAATTGAAACGTACCGCGAAATCGATTCCCGGTACGGTTGCTTGTATTGTTATCCCCTGCGGCTGGTTACTCACCAAGCCGCGACATCGTCTCCAGCGAACCGTTCTTAAGATATAACAGTCATTTTTATAACAACGGATTATCCCCCGTTTACTTGCCCTTTCAATGGTTGTCCACCGCCAGGTGAAATCCGGCATCGTTTGCCTGCCCTTCACGGCGTTGTCATTAATCCGCCAAACGGATACGAAAAGATACCAGACATGCGGAATTAAATTGCGCCAGAGGCGGCGGCGCGACGTGCCAAAATCAACAGAATATTTGTGCTCACTACGGCTCAGTAATAGGTCGGCGATAAACACATCATACCCGATGCAAAAAGGGTTAATGTGACTGGAAATCAAATACATCGCGATTTCGTTAAGCTCAGAAACGCTCTCATAAATTGCGCGAATTTTGGGTTCCACTTCTGCGGTGACGTTTTGATGCGTGCGGGTAACAATAAACGCCGTTCGCTCAGCCATATTGTTTCAAACACAGGATCGCATAGCGGCTCACCGACTTGAGGCCATCATCAAAGGGTAGATCACCACCGCTCGCGCCAGGGGAGCATTGGATCTCTTATTCTCACGTAGCATTCCCAGCTGCACCATCTCAGTGTCGCGTAATCCCCTGTCACGCAGCCGGCCATCGGTTTATTGAAAGCCGTACCATGTGGGTCGTCGAAGTATGGCGAAAAACGGAGTGTTTCTTTTCGCCGGCGCAGAATATGGTGATAGGTTAGCACCGAAGGCCATATTCGGGTTGGGGTCTCCAGTGCGCTGATATAAGACAGCGATCGCCCATAAGGAATCTGATACCAGGTTTGATATAACCTGTCGTTCAGTTATGCAAATCGGGGGTAGCCGCAAATTGTTCGCCGTACCCCCAATGGCGCATTCCCCGCACTCGGCGCGTTATTAGACCGCGTATCTTTCCAGGTACTAAGTTCTGAATTCTCAGCGGCTTGTTGAGGTCGCCCAAACGTTCTTCAACTTTTTTTGCGCCCCTGAACCGGCTCGAGATGACCTTTACGATTAAAAACCTTTTGCCAGAAGCCAAAATTAAATGCGTAATAACATTGCGGCAGTGTCGCTCCACGGCAATGATTTGTGGCTCGGCCCGATATATACCCCACGGTACCATCTTGTCACCATATGGCGCCCAGACCGCCAGCATATTTCGGCATGGTTTGCTATAACGGGCAAGGTAACGCGGCACTGACACTTGCGGTTAAACAGCATCAGCAGGAGAATAACACGCTTGTTTGTCAATGAAAAAGTAACGAGGGTGAAACAAACCGCTTTTATTGGTAGCAAAAAGCCGCCATCAGAATCGCGGGGTTAAATACTCATGAACAAAATTATGTTTGAGACGAAAGGCGGATTTTTTTGTTGTTGGTGCCAAAAAGACTACTCACCTCCGGGCGTACCCAGGTTCCGCAATACGCTCGCGAACTTCTGAACTGGGTCCCATGGTTTCACGAACAAGCATCATCTGTTCAATTAACGTAACGTGACACCAGAAAAATCACTTCGCACGGGGAGTGTTTGTGGCTTAAATAATCGAAGCCACCACGCGATACGGTGTGCGCTTCCGGCTATATCGGTTAAGAAGATGGAGCACCCTACACCGGATGGTGCCGCTTACATTGCAGAAGAGCACCGCACATCATGTCGGCTAGAGCATTAAGCAGCTTAATGCCGGCCAGAACATTCACGGGCCGGCACTGAAGAAGTGCCCCATGTTTTTTTTTCCCAGCGCATCCAGTATCCTCTTGCGTCCCGTCATCGTGTCAGGTAATTACCCCAACCTTAACATCGCCTTCCTCCTAGCCGAAAACAGGTCTTCCCTGACCATTGTTGATAAAGGGTGATAGCCGTCAAAAACTCCTATAGCCCCCTCAAATTCCAAAGGAGAGGAAATAGCCTTAGCTACGCAAACCACGTCCACAGTGGATCAAGCGACCAAACAAATCAAATAAAAACGCCACCAATAAAGACCCACCCAGTACGCCAAAGGGTAGTGAACCAGCGGAACATCATGATTACCGAGAAGCCGTTAGCGGAAACACCTGATCTATTAAACGATTGGGTTCAGGTGCGAACAGGCCCTTGCCAGAAACGGGCGGCAACAAAGTCAGTTGGGTAAAAACCCCGCCAATACGTGAGTGGCGTAACACAAAGGTTGGCACCAGGCCTGATGTCATCGAACGTTTTGCAAACCACCGTTCATGCAAACCGCAAGGGAGAACAACACACCGCCGTGAGCACCAGCGTTTAAGGCAACGAATACCCACGAATGCCCCTGAAATGGCACAAAAAACAGTGAATTCCTCACCACAAGGCCAACAAACAGGACCACGCGCCCCACGCCACCGCCGACATATCCAGGCAATAATGACGTGCGTCGGAACCGGCCTACAGCAGCTCTTCAATATTACGCTGTGAACTTGGACCAAAAAAATGATGACGCAACGTTGGCCGTAGGCATTGGTGATCACCGACATCATGATCAGCCCCGGTACGATGAGAACTGCAATAGCTGAAGCATGCATATCTGCCAATACCGCGAACCAATCAGGGTTACCGAAGATAATAAGTAAAGGGTATGGTGATGACTGCGGCATCAGCGTCTGCACCCAGATACGCATCAACAGCGATGGGATCTCCTCTTCGCCCAGATGCTTTTACGCCACCCAGTAAAGATGCATCATGCGCGACTCCTTGTTTTCATTAAAACCAGTTGAAACAAACGCTCTTCCAAGCACTGGTTAGCTTGTTACGCATATTAAAAACCTGAGCCCTGCTCACTTAACTGCGAAATACGCTGTGTTGATCCCCTGCTTCACGCAGACTTCAACTTCCAGCGTCGCCGGTATCGACCAGGCGAATACTGATAGCCACGAGCTTCGGGGTAACGGGCTTTTCCGGTCGCGAGATCGAGAATAAAGGCTTTTCCGATTTCCAGCTCCCAGTCAGGCGCCTTCATCGAAGGTATTTTCCACCAGCTCCCGGTTGAAAATCGCCGATATTGCGGCACACCATTTCTGCTTCTTCCAGGTAGTGTGTGGGGAGAAGATGGGGTGCCTTTGTCGTTTAAATCCTTCAAAAGGCCCCACATTGAGCGGCGAAGTTCAATATCCACGCACCGCGGTCGGTTACTCGGAGAATCAGCAGTTTGGATTCATCGCATTAACGCACGGGCAATCATTAAACGGGCGCTTTCACCCGGCCAGATAACATACGCCGCACGTTCGTTGCGTTTCCCCCATAGATCCGAGTTGTTTAAGATACTTTTCGCTCGGAGTACGCTTCTTTGCCGCGCCACGCAGTAGTACCCCTGCCTGACTCACCACAATTGCCTCGCACGGTTTCAAACGGGTTGAAGTTAAATTCCTGCGGCACCAGTCCCACTGACGGTTTAGCGTTTCACGACAATCCTTCTCGAGATCGTACCAAATACGCTGACCCGCCCGGAGGTTTTAATTACCAGGAGAGCTGAATAATACGATAGCTGGTCGATTTCCCGCCCCGTTCGGCCCGAGAAGCGCATAATAAATCACCCGCTTCGACCTGGCAAATCTATCCCCACGAAGCGCCTGAAACGCCGCCTGATAGGGTTTTTTAAGCTGTTGAGTTCCAGTGCAATGGTCATAAATTTTACTTACCTTACTTCTACACTTTATCTGTGGTTAAATCGTTCCCGGAGTTGCCCTATATTAGCCAAACGTAATTATTTTGGTTTACCAGTGTCGTTAACTCCATGAAAGACATAGAACACTCACCAGCAACAATGCAACTATGGTCAATAAATGCTGGTGGAGAGGATCCACGGGTTTTTTGAGAAAACTGGCCAAGCGCAAAAACCGCGCTTTCTATGGATTGGTGTTGCCGACAAGTCGCGTTTACCTGCAGAACGTTTAACCGGTCTTTGAGCCGGGAGAACTCTTTCTGTTCACCGTAATGTTTGCTAACCTGGTCATTCACACTGACCGTGAACTGCTTTCCGTGGCTTCAGTATGCAGTGATGTAGCTCGAGGTTGAACACATTATTATCTGTGGCCACTACGGTTGCGGCGGCGACAAGGCCGCAGTTGAAAACCCGGAACTGGGGCTTATACAACTGGCTGCTGCATATCCGCGTATATCTGGTTCAAACATAGCTCATTGCTCGGCGAAATGCCGCAGAGCGCCGTCTGGATACCTTGTGTTAACTGAACGTCATGGGAACGGTGTATAACCTGGGCACCCACCATTATGCATCAGCGTGGAAACGCGGGCAAAAGTTACCATTCACGGCTGGGCCATACGGGCATTCACGCGCTTGCTGCGTGATCTGGATTTTACCGCCACCAACCCCGAAACCCTTGAAGCAACCGTTACCGTCACTGGTTTCCAACCTCAAGCTGTAAACACGCCAACCGCAAAAAATAAAAAACATGCCATGCCGGGATGCAAACAATCCGGCAACTCCACACCTTTATCTTCAGCAGACTCAGCTTTGCCGGATAACGGCAGATGACGGTATACGCTGTGGCGTAATTCAATGCCGTACCCACCCACAAACCTCATCCGGATCGAGAACCGATAAAATTTAACCGGGACGTTCACTCACGAGGACCGGGTTTTATCCAGCAGCGTACAAATCCAGGACTTCGGTTCGCGCAGGCTTAAGACTTACGCACTTTCGACAAGTGTATTCCCCGAGTCCGGATGATATTTAACAATCAGCACGTCCCTTGACCACGGATATCTTCCATCCAGTCTTTGAGGATTTTACATCACGGGTGGTGGACATGCCGGCACCGTAGCTGGAGGCGGTCATAAAGTCGACTTCAGGGATACCTGAACTTCACGGCACAGGTCCGTCGATAAAACTAAATGAGCCACCGGCACAAGACCCACCAGCACCATATTCGCTGCCTGTCATTGTAACGCTCAGTAATCTGACGACCCAGTTCGGCGATACGCGCTTTATCCCGCTTCGGGGATCATTTACTTCTACAGTATGTTTGTCCATATTCTAACCATATGATTTAAAAACAAATCACTTTCGCACGGGCAGGTTTTGGCACCGCAACAAAGCGCAAGACACGCAGTATACCAGTAAAACTATTCAATTCGCGGAATCTGTTAATAAAGCTCGGAATTGTGATGACGATCACACATGTAAAACCCTATAATTAATTTGCTACTAAAATATTAATGAATTGAAATGGTGTCTCTTTTATGGCAATTAACAATACAGGCTCGCGACGATTACTCGTTCACGCTAACAGCCCTTTTTCAGCGCTTTGCGGGCTGTATCTCGTCATTGGCGGAGGGCCTGGCTGGTCGCGATTGGCGGCTCCTGGTACTACCCTATCGCTGGCCTTATGATGCTCGGCGTCGCCTGGATGCCTGTGGCGCAGTAAACGCGCCGCGCTTTGGCTATACGCAGCCCTGCTGCTCGGCACCATGATTTGGGGCGCTGGGAAGTTGGTTTCGACTTCTGGGGCGCTGACTCCGCGCCAGCGACATTCTGGTCTTCTTCGGATCTGGCTGATCCTGCCGTTTGTCTGGCGTCGCCTGGTCATTCTGCCAGCGGCGCAGTTGCCGCACTGGTGCGGGTCGCCTTGCTAATTAGCGGTGGGTATCCTGACCTGGGCCGGATTTACGATCCGCCAGGAGAATCAAAGGCACTTAAGCGCCCGATGCCACACCTGCTGAAGCTATCTCCCCCGTAGCCGATCAAGGACTGGGCCTGCCTATTGGTCGTAATCAGGAAGGTCAACGCTTTTCGCCGCTGAAACAAATTAACGCCGATAACGTCCATAATCTGAAAGAAGCCTGGGTGTTCCGTACTGGCGGATGTGAAGCAGCCGAACGATCCGGGTGAAATCACCAATGAAGTGACGCCGATTAAAGTGGCGACACCCTTTACCTGGTACCGCCTCACCAGCGCCTGTTTGCGCTTGATGCCGGCCAGCGGCAAAGAAAATGGCATTACGATCCTGAGCTGAAAACCAACGAGTCTTTCCAGCACGTAACCTGCCGTGGGTGTCTCTTAATCATGAAGCCACCAAAGCAGAAACCGCTTCGCCGAAGTGGATGGCGATTGCCCGCGTCGTATCATTCTTCCGGTCAATGATGGTCGACTGATTGCGATTAACGCTGAAAACGGGCAAACTGGTCGAAACCTTCGCCAATAAAGGCGGTGCTAATCTGCAAAGCCAATATGCCAGACACCAAACCGGGTCTGTATGAACCGACTTCGCCACCGATTATCACCGATAAAACCATCTGATGGCCGGTTCAGTTACCGATAACTTCTCAACCCGCAAACGTCTGGCGTGATCCGTGGTTTTGAGATGTCAACACCGGGGAGCTGCTTGTGGGCTTTTGATCCCGGCGGGAAAGATCCGAACGCAATCCCGTCTGACCGACACACCTTTAACCTTTAACTCGCCAAACTCCTCTGGGCACCAGCGGCCTATGACGGCGAACTGGATTTCTGGGTCTATCTGCCGATGGGCGTGACCACGCCGGATATCTGGGGGCGGTAACCGCACACCCGGAACAGAACGTTTGCCAGCTCGATTCTGGCGCTGAATGCCACTACCGGGAACTGGCGTGGAGGCTACCCAGACCGTCAACCACGACCTGTGGGACATGGATCTTCCGGCACAGCCGACGCTGGCGGACATCACCGTTATATGGTCAGAAAGTGCCAGTTATTTACGCTCCGGCGAAAACCGGCAACAATTTTTCGTGCCTCGATCGTCGTATGGCGAACTGGTGGTTCCGGCACCGGAAAAAACCGGTTCCCAAGGTGCAGCGAAAGGCGATTACGTAACCCCAAACTCAACCGTTTTCTGAACTGAGCTTCCGTCCGACGAAAGATTTGAGCGGTGCGGATATGTGGGGGAGCCACCATGTTTGACCAATGGTGGTGCCGCGTGATGTTCCACCAGATGCGCTGAAGGCATTTTCACCCCGCCATCTAGAACAGGGTACGGCTGGTCCTTCCCGGGTAACCTGGGGATGTTCGAATGGGGCGGGATTTCCGTTGATCCAAAATCGTGAAGGGGCGATTTCAACCCAATGGCACTGCCCGTTTGTTTTCGAAACTGATCCCGCGTGGTCCTGGCGAACCCGATTGGAGCAGCCGAAAGATGCCAAAGGCCACGGGTACGGAATCCGGCAGATTCAGCCCAGTACGGTGTACCGTATGGGTGTCACGCTCAACCCGATCCTCTCACCATTTGGTCTGCCATGTAAAACGCCAGCATGGGGTTATATCTCGGGCGCTGGATCTGAAAAACTCATGAAGTGGTTGGGAAGAAACGTATTGGTACGCCGCAGGACGTATGCGGTTCCCGATGCCGGTTCCCGTGCCGTTCAATATGGGTATGCCGATGCTGGGCGGGCCAATCTCCCGGCGGGTAACGTGCTGTTTATCGCCGCTACGGCAGATAACTACCGCGCGCTTACAACATGTGAGCAACGGTAGAAAAACTGTGGCAGGGTCGTTTACCCAGCGCGGGTGTCAGGTTACGCCAATGACCTATAGAAGTGATGGTTAAGCAGTATGTCGGTGATCTCCGGCAGGCGCAGGTTCATTTGGGTACGAGATGGGCGACTATATTCGTGGCTTATGCGCTGCCGGATGTGTGAAGTAAGACTTGCTCAGTTTGCTGACAACGTGCGCGTGTTCATGCCGAGCGGCGTGATCGCCTTATCCAGCCTACAAATTGTGCAAAATCGATGGATTGCACAGCTAGCGTAGGCCTGTAAGCGAGCGCATTCGGCAGATTTGCGTTGTCATCAGTTTTAATGCCCGGAGAGATCCGGGCATATATTTCCGAATACGGTCTTTTTATACCGTAAACCCTAACATCATCCCCGTATCTTCATGCTCCAGCAGAGGCAGTGCGCCATGATAAGCATGTCTTTCGGTGCATCGTGATTAAACTTCACCAGGCACTTTCGCTGACATTACTTCTACCTTAACGGTATCTTTCCAGCCCGCGCGATGAGCCGCTGGCGGTTTGCCATTTTCTGACAAGATACGGAACTGCGTGCCGTGGATATGGAACGGATGCCAGCATCATGTCGGCCCCCCAGAGAAACCCAACGTTCGTATTGCCCTTTCGCCGCCGCAAACATCGGCTTCTTCATATCAAACGCCTGACCGTTGATTTTGTTGGCATGGTGTTAAAACCCTGCGAACTTCCCGCCGTGGTTCATAATGATTCATATTGCCGGTGCCCCATATGGCCCATCATCTGGCTGTGATCCATCCCGGCCATCGCCTGATCGCCAATTTCTCCTTAGCATCTGCATCCCCATCATATCGAGGCATCGGGTCCATAGAAGAGATTGCAGCTTGCGACCGTCAGCCCTTCCAGCGAAAGGTAAGGCAGGCAGGCTACTTAAATGTGTCTGGCAAAGCACCGGAGGCACTAATAGCAATTCGGCTGAATCCGCATTACCGGATGGAGGCTTATCAAACGGCGCAATCGCCATCCCCATCTGGCTGACCGGCAGCGTCACCGGTCAAAGGGTTTGTGTATCGTTAACCCTCCACCAGACCACTTCAAAACGCTCGCCCATCAGCACCGGCAGTTCAGCTCACCTTCACTGGTTCAGGTAGCAGACCACCCGTCGCTGGCAATCAATACAGCGGGCGATTGTCGCTGGTGCGAAATTGAGCGGAACGGGCATTACCCCCATTGAGCAAACGCAGGCGCAGCCAACCACGCGGGGCAGCGTGTTGCGGGTAGTTGCACCGTTGGTACAGCAACGTATCGCCAAACCAGCCCACGGCGGCGGTCATCACATCCAGTTGATAATCAATCTGCCCGTGGGCGCTAAAATTTCTTATCCTGACGACATACCGGAACATCATCGATACCCCACTGTTTTGGCAGCATTAAATTTCAGGATCTCCGTTCATCTTCAATCACCACCAGCCCAGCCAGCCCCCATCGCCACCTGTCGCCCGGTTTTGCCGTGCTGATGCGGATGGAAAACCAGCAGGTAGCGGCAGGTTGATCAACGGTTCAACGTCACCGAAGCGCTTGCCACCTGGCGGAATAATTCCCTGCGGGCCGCCGTCGCGACTGTTCACCCGGTACTTCCACCCGTGCCAGTGCAACGTTGTCTCTTCCGTCAGTTGGTTGTAGATATCAACCAGTTCCGCTTGCCGCGCTGTAATTTCACCGCCGGCCCCAGCAGTTTGCCGTTATAGCCCCAGGTAGTTGCAGTTTTCCCGCCAAAGGCGGACTGGCCTGCGCCAATAGTTAACTCGAATGCGATTACGGGCATCGGTCGTGAGCAAATCAGGGATCGGGAACGTTGGGCGTTTGCCGCAAATACTGCGCGGCTCCACAGCGGCAAAGCCCGAAGCCACACCCAGCGCGACGGAATATTTTAAGAAATCACGACGTTGCATAGTTATTTCCTTATTCTTAAGCAGGCGAAACAAAATCAAAACGTTTGAGCATAGTCCTTCCCCCCTTACGGGAAGGTCAAGCCGCAGACACAATTTAATTCGACAATAAATGGTCGTCGCCTCGCTCGCAGTGTGCTAACGTTTATCTTCTTTCAGCCCCTGGTAGAAGCAATGAGAAGACGTTTTTCAGAACAGTGTTATTCGGCAGCCTGATGCCGTCTGCGCAAACAGTTCGCGTACAGGAGTCTGAAGCCGAAGATATGGCCGATTTAAACGGCAGTTTTGTCTTTCTGAAGAACGATTGTGGTTACCAGAACTTACCTAAACGGGCAAATTCGTCGCGCACTGGTCTTTTTCGCTCAGCAAAACCAGTGGGCCTCAGTAATTACGACACCTTCGACCATGAAAGTCCCTGGTGAAGACAGCTACGCGATCTCAGCGGCATTGGCATTCCCGTCGCTAAAAAATGCAAAGCCCCTGGCCCGCGATTCCTTAAGCCTGCTTTGCCTACGCAAAGTAATCCCTGATACCTTTTCGTAGAGAATATTGACCGTGCATCCGCGTTAATGGTTAGCTATTATGTTGCGCCCTTTTTTTACGGTGTTTAACAAAGGAGGTATCAACCCATGGCCGAAAAAAAACAGTGGCATGAAACGCTACACGACCAGTTTGGGCAGTACTTTGCGGTAGATAAGTTCCTGTATCCATGAAAAGACCGATCACCAGGATCTGATCATTTTTGAGAACGCTGCATTTGGTGTCCCGTAATGGCCTGGATGGCGTAGTACAAACCACCGAGCGCGACGAGTTTATCTGATCATGAGATGATGACCCATGTTCCGCTACTGGCCCCATGGTCACGCGAAACATGTGCTGATTATCGCGGCGGCGACGGTGCCATGCTGCGCTGAAGTAACCCGTACATAAAAACGTGAGTCCAATCACGAATGGTGGAAATCGATTGCGGGTGTCGATCGTTCTGCCGTCAGTATCTACCCAACCATAACGCCGGTAGCTACGACGATCCGCGCTTTAAGCTGGTGATTCGACGATGGCGTCAATTTCGTTAATCAAACCAGCCAGACCTTTGATGTCATTACTCCGGACTAGCACCGATCATATCGGTCCCGGCGAAAGCCTTTCACTTCGGCATTTTTAGGAAGGCTGCAAACGTTGCCTGAATCCTGGCGGTATTCTTCGTCGCACAAAACGGCGTCGCTTTTTACAGCAGGAAGAAGCCATCGACAGCCATCGCAAACTCAGCATTTCTTTCAGCGACGTTTTGGGCTTTTATCAGGCGGCGATCCCGACCTATTAACGGCGGTATGCATGACTTTTTGCATGGGCGACAGATAACGACGCCCTTACGCCAACTCCTCAACCGAAATTATTCAGGCGCGTTTTCTCGCTCTGGCCTGAAATGGCCGTTATACAATCCGGCAATCCATACGCCCAGCTTTTGCCTTACCTAAGTATCTGCAAGACGCACTGGCTTCACAGCCGTCCTAAGAGAAAGATAAAAATTAAAAAACTGAAACTGCATGGCTATTAATAATCTGACCAAAAGTCTGAGTTTTTGTATTTACGATATCTGGCTACGCCAAAACTGCCGAAGAGCGCGACGGTATATTGCTTATACTCGATGAGACTCTATAATGCCCAACCGTCTGACCGAAATCCTGTCAGAAACCTGTTCCATTATCGGGGCTAAATTTCTTAACATCGCCCGCCAGGATTACGACCACAGGGTGCCAGCGTCACTATTCTGGTAAGTGAAGAACCGGTTGACCCGAAACTCATCGACAAAACAGAACACCCCGGGCCCACTGCCAGAAACGGTCGTTGCCCATCTTTGATAAAAGTCATATTTGCGTACACATACCTTACCCGGAAAGTCATCCTGAAGGCGGTTTATGTACCTTCCGCGCCGTATATTGCAAGTCTCTACCTGCGGCGTGATTTCTCCGCTGAAGGCGCTTGAATTACCTGATCCACCCAGCTTTGAGTCCGATATCGTAACCCATTGTTTATCGCGTGCGCGGTTTACCGCGAACATTAACGGTTATTGAAGCACTTTATCGACCATGAGATTAATTCATTCAAAACTTTATGTCTACGATATGACGGCGCTGTAGACATGGTGGATCGAACGTCTATCAGGAAAATATCTTCCAACCAAGATGTTGCTTAAAAAGTTCGACCTTTAAGCACTACATGTTCCACACCAAACCGGACAGACGTTAACCGACAGCGAGCGCCAGGAAATTACCGCTGCGCTGTGGAAAGAAATTGCGCGAGAGTTTTATTACGGGAGCAATATGCCAGCTGTTTAACGGCTCTGGCGGAGCTCCCAGGCTCCGCCAGATTTATTACTTCTGCTGCACGAAATTGCGGTAAGCCGCCACGACCTGCAGAAAATCCCCAACGCCGCATAGCGACAGGCTTTACTTCGTCGTACAGTAGTAAGTTCATCCCCTTCTTCCATTTCATCGCCGCGAATTCCAGTTGATTGGCGCGAACCGATCCCCTCTTCACCGTCCTCCACAGGGTGTATTCACTGCCCTGCCCGTTGCCAAGGACGTTCGCTACCTTTCAGTGCGTGCGCTGCTTGTTCCACTTCATCAAGCAAGGCCAGGTTTTCTTTTCACCTCTTCATTAAAACCAGTGCCGACCACTTCATCTGCGCCCAATGGACATACGCACCTTTACCACTCCGGTAATATCGCGCAGAAATTTGTAATCCCATAGTGTGTTCCTCTGCCCCGGCAATGCACCGAAATCGCATTGCGCCATTGATGTAATTATCGCAGCCTCCCCGGAGAAAAACAGCGGAACGGCGATGGTTTAGAAATAAAAAAATGCCCGGAAATCTCATTCACCGGGCATTGTGTCGTTTATGCGCAGCGCGTGCGGCTGACTTTTTAAACCGCAGTTGGAAATCACCCCATCGCTTTCTCGGTGTACTGAGAAAAGCTGGTTGAAGTTCAGATAACGGTAAGTATCAACGGCTGTTTTATCTACCTGCGCCACGTAGGGTCTGTACTCTTCCGGCGGTCGGCAGTTTGCCAATCAGCGCCGCAACACCGCCCCAGTTCCGCAGAAGCCAGGGAAGACATTCGCGCCAGTACCCAGACGGTTCTGGGAAGTTTACGGGGTAGAGGTGGGAAACCACCGTTGCACCGTCCGCCACACGCGCCTGGTTACCCATACACAGGAACAGCCAGGGATTCTCATACGCGCACCACTCTTACCCGAAGACGCTGTAGTAGCCTTCTTCGGTCAACTGTGCGGCGTCCATACGGGTTGGCCGGTGCCACCCACAGGCGGGTCGGCAACTACCTTTATGCGCATCCAGCAGTTTTACCCGCAGCACCGGAAGTGACCGATGTTTGGTCATGCAGGAACCGATAAACACTTCGTCGATCTTCTCACCCTGTACCAGCAGACAGCGGACGCGCGTCATCCGGGTCCGTTCGGGAGCACACAGGATTGGCTCTTTAAATACTCCGCCAGATCGATGTCGATCACTGCCGCGTATTCCGCATCTGCATCGGCTTCCAGCAGCTCAGGATTCGCCAGCCATTTTCCCATGCCCTGAAATACGACGTTCCAGGGCTACGACGATCGCCGGTAACCTTCGCGATCATCCACCTTCAGCAGGACCATGGTTAGGAGTCGGTTATTCGATGAATCGGTTCTTCTTGTTCAGCCCTTGATGGTACACCCAGCGGCAGAACGCTCGGCGGGGAGCATCGGTTAGCTCAAGGCGCCTGCTAACTTTCAAAGATCACGCAGTCCTTTCAATTTCCAGGATGCGGCCCAGAGAAGATGTTTTTCTTGCCTTCCTTTCTCAACGGTCAGCAGACCTTGTGACGCATACGCGGAATAGCGGTGACCAGATCCGCGCGAGGGTGATGCGCCGGCTGCATTATTGCTCTTTGAAGCGCACCAGAACGGATCCGGATATCAATGGCGGCGATTTACGCCAGGTTGCGGCAGCAAACGCCACCCAGACCAGAACCCGCCGGGAAAGGAGATACCGACGGGGAAACGGGTATGGCGAGTCACCACCGGACCGACGTATCCGGCGCCAGCATACGGTTCAGCCAGGAGTGAATGACGCCGTCGGCCCGAACGCAGCGACACACCGCCACGGTTCATAAATTGAAGTCCGCGGCAGCGTGTGGTGCGTGTTCACGTCAACTTGCCTCGGATACGCGCGTGTGGCAGAAAGACTGCATCACCAGGTCAGCCGAGAAGCCCAGGCCGCCAAGGTCTTTCAGTTTCATCACGGGTCATGGGCCGGTGGTGTCCTGGGACCACTGAAGTCATTTTCGGTTCACATACCGCCCGGACGAAGTGCCTTTCAACGCCCACAGGCTCGGCCTACACTTTTTTTGGCCAGCGAGAAGCCGCGATCGCATTCGCGCACATCTTTCGCCTGACGGAAGCACATCCACTTGTGCGGCAGACCAAGTGCTTCACGCGCTATGGTGGTCAGGCCACGCCCGATAATCAGCGGAATACGGCCCACCAGCACGCACTTCATCAATCAGCAACGGCGGTTTTCAGTTCGAAGGTCGCCAGCACGTTCGCCGGTTTCGTGGTTACGCACTTCACCTTTGTGACGGGTAAACGTCAATCACGTCGCCCATGTTCAGGGTTAGGACGTTCGCAAACTTCGATTGCAGGCACCCAGCTGTCTTCCATCCTGCATTTAAGAAAGATGGGTTGCAATTTTACCGCCGAGGCTCAAACCACCGCCGCGTTTTGTTCGCACATGTGGGAACATCATACGCCCCATAACCCAGAACGGAGTCTAGTGGCGGTGATTTACGCCGAAGAACCCGGTACCCAACAACGTCACCGACGTACGCCCAGCGGGAAACATTTCTGTTGCAGAGCTTCGGATTTGCTTGAATCGGACCAACAACACCAGGCTGGTCTGGCTCAATACCTTCACGGGGCGTTTTTTCAGCATCGCCGCGCGTGGCAGTGGGATATCCGGGCGTGGACCAACGCATCCGGAGCCGGAAAAGTTCATCGAGTGTTAGTTTCGCCAGTGACTTTGAAGACAGTAAACGGTTGTTTTTCCAGCAGCGGCCGGGGCGATTGAGGACCATTCGGCACCTCCGCCCAGGACTGCATAACCTGCTTCGCATAATTCGTTGCCCTGCTTTTCGCTTTCTCTTCTACGTTTAGA diff --git a/workflows/genome-assembly/polish-with-long-reads/test-data/long_reads.fastqsanger.gz b/workflows/genome-assembly/polish-with-long-reads/test-data/long_reads.fastqsanger.gz new file mode 100644 index 000000000..2a61b4c51 Binary files /dev/null and b/workflows/genome-assembly/polish-with-long-reads/test-data/long_reads.fastqsanger.gz differ diff --git a/workflows/imaging/fluorescence-nuclei-segmentation-and-counting/.dockstore.yml b/workflows/imaging/fluorescence-nuclei-segmentation-and-counting/.dockstore.yml new file mode 100644 index 000000000..e51c75ebe --- /dev/null +++ b/workflows/imaging/fluorescence-nuclei-segmentation-and-counting/.dockstore.yml @@ -0,0 +1,12 @@ +version: 1.2 +workflows: +- name: main + subclass: Galaxy + publish: true + primaryDescriptorPath: /segmentation-and-counting.ga + testParameterFiles: + - /segmentation-and-counting-tests.yml + authors: + - name: Leonid Kostrykin + orcid: 0000-0003-1323-3762 + url: https://github.com/kostrykin/ diff --git a/workflows/imaging/fluorescence-nuclei-segmentation-and-counting/CHANGELOG.md b/workflows/imaging/fluorescence-nuclei-segmentation-and-counting/CHANGELOG.md new file mode 100644 index 000000000..2169bdc88 --- /dev/null +++ b/workflows/imaging/fluorescence-nuclei-segmentation-and-counting/CHANGELOG.md @@ -0,0 +1,5 @@ +# Changelog + +## [0.1] - 2024-02-29 + +- Creation of workflow for segmentation and counting of cell nuclei in fluorescence microscopy images. diff --git a/workflows/imaging/fluorescence-nuclei-segmentation-and-counting/README.md b/workflows/imaging/fluorescence-nuclei-segmentation-and-counting/README.md new file mode 100644 index 000000000..60d0a1234 --- /dev/null +++ b/workflows/imaging/fluorescence-nuclei-segmentation-and-counting/README.md @@ -0,0 +1,17 @@ +# Segmentation and counting of cell nuclei in fluorescence microscopy images + +This workflow performs segmentation and counting of cell nuclei using fluorescence microscopy images. The segmentation step is performed using Otsu thresholding (Otsu, 1979). The workflow is based on the tutorial: https://training.galaxyproject.org/training-material/topics/imaging/tutorials/imaging-introduction/tutorial.html + +![](test-data/overlay_image.png) + +## Inputs + +**`input_image`:** The fluorescence microscopy images to be segmented. Must be the single image channel, which contains the cell nuclei. + +## Outputs + +**`overlay_image`:** An overlay of the original image and the outlines of the segmentated objects, each also annotated with a unique number. + +**`objects_count`:** Table with a single column `objects` and a single row (the actual number of objects). + +**`label_image`:** The segmentation result (label map, which contains a unique label for each segmented object). diff --git a/workflows/imaging/fluorescence-nuclei-segmentation-and-counting/segmentation-and-counting-tests.yml b/workflows/imaging/fluorescence-nuclei-segmentation-and-counting/segmentation-and-counting-tests.yml new file mode 100644 index 000000000..35facddc5 --- /dev/null +++ b/workflows/imaging/fluorescence-nuclei-segmentation-and-counting/segmentation-and-counting-tests.yml @@ -0,0 +1,19 @@ +- doc: Test outline for segmentation-and-counting.ga + job: + input_image: + class: File + path: test-data/input_image.tiff + filetype: tiff + outputs: + overlay_image: + path: test-data/overlay_image.png + compare: sim_size + delta: 100 + objects_count: + path: test-data/objects_count.tabular + compare: diff + lines_diff: 0 + label_image: + path: test-data/label_image.tiff + compare: sim_size + delta: 100 diff --git a/workflows/imaging/fluorescence-nuclei-segmentation-and-counting/segmentation-and-counting.ga b/workflows/imaging/fluorescence-nuclei-segmentation-and-counting/segmentation-and-counting.ga new file mode 100644 index 000000000..962daf4de --- /dev/null +++ b/workflows/imaging/fluorescence-nuclei-segmentation-and-counting/segmentation-and-counting.ga @@ -0,0 +1,343 @@ +{ + "a_galaxy_workflow": "true", + "annotation": "This workflow performs segmentation and counting of cell nuclei using fluorescence microscopy images. 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b/workflows/imaging/fluorescence-nuclei-segmentation-and-counting/test-data/input_image.tiff new file mode 100644 index 000000000..0321addb2 Binary files /dev/null and b/workflows/imaging/fluorescence-nuclei-segmentation-and-counting/test-data/input_image.tiff differ diff --git a/workflows/imaging/fluorescence-nuclei-segmentation-and-counting/test-data/label_image.tiff b/workflows/imaging/fluorescence-nuclei-segmentation-and-counting/test-data/label_image.tiff new file mode 100644 index 000000000..bce30bc66 Binary files /dev/null and b/workflows/imaging/fluorescence-nuclei-segmentation-and-counting/test-data/label_image.tiff differ diff --git a/workflows/imaging/fluorescence-nuclei-segmentation-and-counting/test-data/objects_count.tabular b/workflows/imaging/fluorescence-nuclei-segmentation-and-counting/test-data/objects_count.tabular new file mode 100644 index 000000000..1d8cebc77 --- /dev/null +++ b/workflows/imaging/fluorescence-nuclei-segmentation-and-counting/test-data/objects_count.tabular @@ -0,0 +1,2 @@ +objects +20 \ No newline at end of file diff --git a/workflows/imaging/fluorescence-nuclei-segmentation-and-counting/test-data/overlay_image.png b/workflows/imaging/fluorescence-nuclei-segmentation-and-counting/test-data/overlay_image.png new file mode 100644 index 000000000..1d8d5b327 Binary files /dev/null and b/workflows/imaging/fluorescence-nuclei-segmentation-and-counting/test-data/overlay_image.png differ diff --git a/workflows/metabomics/gcms-metams/.dockstore.yml b/workflows/metabomics/gcms-metams/.dockstore.yml new file mode 100644 index 000000000..7615ccdbd --- /dev/null +++ b/workflows/metabomics/gcms-metams/.dockstore.yml @@ -0,0 +1,12 @@ +version: 1.2 +workflows: +- name: main + subclass: Galaxy + publish: true + primaryDescriptorPath: /Mass-spectrometry__GCMS-with-metaMS.ga + testParameterFiles: + - /Mass-spectrometry__GCMS-with-metaMS-tests.yml + authors: + - name: workflow4metabolomics + image: https://raw.githubusercontent.com/workflow4metabolomics/workflow4metabolomics/master/images/logo/logo_w4m-0.1-black-orange.png + url: https://workflow4metabolomics.org/ diff --git a/workflows/metabomics/gcms-metams/CHANGELOG.md b/workflows/metabomics/gcms-metams/CHANGELOG.md new file mode 100644 index 000000000..38910c77d --- /dev/null +++ b/workflows/metabomics/gcms-metams/CHANGELOG.md @@ -0,0 +1,7 @@ +# Changelog + +All notable changes to this project will be documented in this file. + +## [0.1] - 2023-11-22 + +First release diff --git a/workflows/metabomics/gcms-metams/Mass-spectrometry__GCMS-with-metaMS-tests.yml b/workflows/metabomics/gcms-metams/Mass-spectrometry__GCMS-with-metaMS-tests.yml new file mode 100644 index 000000000..d85e6aaf6 --- /dev/null +++ b/workflows/metabomics/gcms-metams/Mass-spectrometry__GCMS-with-metaMS-tests.yml @@ -0,0 +1,54 @@ +- doc: Test outline for Mass-spectrometry__GCMS-with-metaMS.ga + job: + sampleMetadata: + class: File + location: https://zenodo.org/record/3631074/files/sampleMetadata.tsv + filetype: tabular + Mass-spectrometry Dataset Collection: + class: Collection + collection_type: list + elements: + - class: File + identifier: alg11.mzData + location: https://zenodo.org/record/3631074/files/alg11.mzData + filetype: mzdata + - class: File + identifier: alg2.mzData + location: https://zenodo.org/record/3631074/files/alg2.mzData + filetype: mzdata + - class: File + identifier: alg3.mzData + location: https://zenodo.org/record/3631074/files/alg3.mzData + filetype: mzdata + - class: File + identifier: alg7.mzData + location: https://zenodo.org/record/3631074/files/alg7.mzData + filetype: mzdata + - class: File + identifier: alg8.mzData + location: https://zenodo.org/record/3631074/files/alg8.mzData + filetype: mzdata + - class: File + identifier: alg9.mzData + location: https://zenodo.org/record/3631074/files/alg9.mzData + filetype: mzdata + outputs: + metaMS.runGC dataMatrix: + path: test-data/metaMS.runGC_dataMatrix.tabular + lines_diff: 10 + Multivariate variableMetadata: + asserts: + - that: has_n_lines + n: 42 + - that: has_text + text: "Unknown 1\tUnknown\t0.0046\t19.499\t-0.17" + - that: has_text + text: "Unknown 41\tUnknown\t0.0039\t14.857\t-0.08" + Multivariate sampleMetadata: + asserts: + - that: has_n_lines + n: 7 + - that: has_text + text: "alg11\tFWS_100perNaCl\t-6.2" + - that: has_text + text: "alg8\tFWS_5percNaCL\t-0.6" diff --git a/workflows/metabomics/gcms-metams/Mass-spectrometry__GCMS-with-metaMS.ga b/workflows/metabomics/gcms-metams/Mass-spectrometry__GCMS-with-metaMS.ga new file mode 100644 index 000000000..1c2ce685c --- /dev/null +++ b/workflows/metabomics/gcms-metams/Mass-spectrometry__GCMS-with-metaMS.ga @@ -0,0 +1,469 @@ +{ + "a_galaxy_workflow": "true", + "annotation": "This workflow is composed with the XCMS tool R package (Smith, C.A. 2006) able to extract and the metaMS R package (Wehrens, R 2014) for the field of untargeted metabolomics. \n\nhttps://training.galaxyproject.org/training-material/topics/metabolomics/tutorials/gcms/tutorial.html", + "creator": [ + { + "class": "Organization", + "image": "https://raw.githubusercontent.com/workflow4metabolomics/workflow4metabolomics/master/images/logo/logo_w4m-0.1-black-orange.png", + "name": "workflow4metabolomics", + "url": "https://workflow4metabolomics.org/" + } + ], + "format-version": "0.1", + "release": "0.1", + "license": "MIT", + "name": "Mass spectrometry: GCMS with metaMS", + "steps": { + "0": { + "annotation": "", + "content_id": null, + "errors": null, + "id": 0, + "input_connections": {}, + "inputs": [ + { + "description": "", + "name": "Mass-spectrometry Dataset Collection" + } + ], + "label": "Mass-spectrometry Dataset Collection", + "name": "Input dataset collection", + "outputs": [], + "position": { + "left": 15, + "top": 101 + }, + "tool_id": null, + "tool_state": "{\"optional\": false, \"tag\": null, \"collection_type\": \"list\"}", + 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workflow is composed with the XCMS tool R package [(Smith, C.A. 2006)](https://bioconductor.org/packages/release/bioc/html/xcms.html) to extract and the metaMS R package [(Wehrens, R 2014)](https://bioconductor.org/packages/release/bioc/html/metaMS.html) for the field of untargeted metabolomics. + +🎓 For more information see the [Galaxy Training Network tutorial: Mass spectrometry: GC-MS analysis with metaMS package](https://training.galaxyproject.org/training-material/topics/metabolomics/tutorials/gcms/tutorial.html) + +## Inputs +### sampleMetadata +The sampleMetadata tabular file corresponds to a table containing information about your samples + +A sample metadata file contains various information for each of your raw files: +- Classes which will be used during the preprocessing steps +- Analytical batches which will be useful for a batch correction step, along with sample types (pool/sample) and injection order +- Different experimental conditions which can be used for statistics +- Any information about samples that you want to keep, in a column format + +The content of your sample metadata file has to be filled by you, since it is not contained in your raw data. Note that you can either: +- Upload an existing metadata file +- Use a template to create one (because it can be painful to get the sample list without misspelling or omission) + - Generate a template with the `xcms get a sampleMetadata file` tool available in Galaxy + - Fill it using your favorite table editor (Excel, LibreOffice) + - Upload it within Galaxy + +**Formats:** tab-separated values as tsv, tab, txt, ... + +### Mass-spectrometry Dataset Collection +Mass-spectrometry data files gathered in a Galaxy Dataser Collection + +**Formats:** open format as mzXML, mzMl, mzData and netCDF + +## Main steps +1. MSnbase readMSData: read the mzXML and prepare for xcms +2. XCMS findChromPeaks: peak picking +3. metaMS.runGC: definition of pseudo-spectra diff --git a/workflows/metabomics/gcms-metams/test-data/metaMS.runGC_dataMatrix.tabular b/workflows/metabomics/gcms-metams/test-data/metaMS.runGC_dataMatrix.tabular new file mode 100644 index 000000000..1e5a6bf1b --- /dev/null +++ b/workflows/metabomics/gcms-metams/test-data/metaMS.runGC_dataMatrix.tabular @@ -0,0 +1,42 @@ +Name alg11 alg2 alg3 alg7 alg8 alg9 +Unknown 1 171208192 19403078 51689436 196528995 111241302 180946294 +Unknown 2 85088688 1386827 4976677 10589259 8917809 11005200 +Unknown 3 75099028 5873408 16302374 56198912 40418507 47836465 +Unknown 4 73997431 2895358 8986693 23638072 18739515 64542302 +Unknown 5 49891456 0 0 57808911 34317665 36298101 +Unknown 6 46647944 0 0 64737554 47489338 44537215 +Unknown 7 39902198 860385 4363914 4717721 1450738 48186709 +Unknown 8 28189000 0 0 11992763 20790174 19217984 +Unknown 9 25076752 0 0 9137481 19261173 18374954 +Unknown 10 21548640 147001 68652 0 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workflow4metabolomics + image: https://raw.githubusercontent.com/workflow4metabolomics/workflow4metabolomics/master/images/logo/logo_w4m-0.1-black-orange.png + url: https://workflow4metabolomics.org diff --git a/workflows/metabomics/lcms-preprocessing/CHANGELOG.md b/workflows/metabomics/lcms-preprocessing/CHANGELOG.md new file mode 100644 index 000000000..6598368b9 --- /dev/null +++ b/workflows/metabomics/lcms-preprocessing/CHANGELOG.md @@ -0,0 +1,8 @@ +# Changelog + +All notable changes to this project will be documented in this file. + +## [1.0] - 2023-11-22 + +First release + diff --git a/workflows/metabomics/lcms-preprocessing/Mass_spectrometry__LC-MS_preprocessing_with_XCMS-tests.yml b/workflows/metabomics/lcms-preprocessing/Mass_spectrometry__LC-MS_preprocessing_with_XCMS-tests.yml new file mode 100644 index 000000000..e9951ff53 --- /dev/null +++ b/workflows/metabomics/lcms-preprocessing/Mass_spectrometry__LC-MS_preprocessing_with_XCMS-tests.yml @@ -0,0 +1,64 @@ +- doc: Test outline for Mass_spectrometry__LC-MS_preprocessing_with_XCMS + job: + SampleMetadata: + class: File + identifier: sampleMetadata.tsv + location: https://zenodo.org/records/10130758/files/sampleMetadata_12samp_completed.tsv + filetype: tabular + Mass-spectrometry Dataset Collection: + class: Collection + collection_type: list + elements: + - class: File + identifier: Blanc05.mzML + location: https://zenodo.org/record/10130758/files/Blanc05.mzML + filetype: mzml + - class: File + identifier: Blanc10.mzML + location: https://zenodo.org/record/10130758/files/Blanc10.mzML + filetype: mzml + - class: File + identifier: Blanc16.mzML + location: https://zenodo.org/record/10130758/files/Blanc16.mzML + filetype: mzml + - class: File + identifier: HU_neg_048.mzML + location: https://zenodo.org/record/10130758/files/HU_neg_048.mzML + filetype: mzml + - class: File + identifier: HU_neg_090.mzML + location: https://zenodo.org/record/10130758/files/HU_neg_090.mzML + filetype: mzml + - class: File + 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b/workflows/metabomics/lcms-preprocessing/README.md new file mode 100644 index 000000000..390aa0aa3 --- /dev/null +++ b/workflows/metabomics/lcms-preprocessing/README.md @@ -0,0 +1,37 @@ +# Mass spectrometry: LC-MS preprocessing with XCMS + +This workflow uses the XCMS tool R package [(Smith, C.A. 2006)](https://bioconductor.org/packages/release/bioc/html/xcms.html) to extract, filter, align and fill gaps, and uses the CAMERA R package [(Kuhl, C 2012)](https://bioconductor.org/packages/release/bioc/html/CAMERA.html) to annotate isotopes, adducts and fragments. + +🎓 For more information see the [Galaxy Training Network tutorial: Mass spectrometry: LC-MS preprocessing with XCMS](https://training.galaxyproject.org/training-material/topics/metabolomics/tutorials/lcms-preprocessing/tutorial.html) + +## Inputs +### sampleMetadata +The sampleMetadata tabular file corresponds to a table containing information about your samples + +A sample metadata file contains various information for each of your raw files: +- Classes which will be used during the preprocessing steps +- Analytical batches which will be useful for a batch correction step, along with sample types (pool/sample) and injection order +- Different experimental conditions which can be used for statistics +- Any information about samples that you want to keep, in a column format + +The content of your sample metadata file has to be filled by you, since it is not contained in your raw data. Note that you can either: +- Upload an existing metadata file +- Use a template to create one (because it can be painful to get the sample list without misspelling or omission) + - Generate a template with the `xcms get a sampleMetadata file` tool available in Galaxy + - Fill it using your favorite table editor (Excel, LibreOffice) + - Upload it within Galaxy + +**Formats:** tab-separated values as tsv, tab, txt, ... + +### Mass-spectrometry Dataset Collection +Mass-spectrometry data files gathered in a Galaxy Dataser Collection + +**Formats:** open format as mzXML, mzMl, mzData and netCDF + +## Main steps +1. MSnbase readMSData: read the mzXML and prepare for xcms +2. XCMS findChromPeaks: peak picking +3. XCMS groupChromPeaks: determining shared ions across samples +4. XCMS adjustRtime: retention time correction +5. XCMS fillChromPeaks: integrating areas of missing peaks +6. 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b/workflows/proteomics/openms-metaprosip/.dockstore.yml new file mode 100644 index 000000000..6f5537223 --- /dev/null +++ b/workflows/proteomics/openms-metaprosip/.dockstore.yml @@ -0,0 +1,11 @@ +version: 1.2 +workflows: +- name: main + primaryDescriptorPath: /metaprosip.ga + subclass: Galaxy + publish: true + testParameterFiles: + - /metaprosip-tests.yml + authors: + - name: Matthias Bernt + orcid: 0000-0003-3763-0797 diff --git a/workflows/proteomics/openms-metaprosip/.workflowhub.yml b/workflows/proteomics/openms-metaprosip/.workflowhub.yml new file mode 100644 index 000000000..28b3e2b22 --- /dev/null +++ b/workflows/proteomics/openms-metaprosip/.workflowhub.yml @@ -0,0 +1,5 @@ +version: '0.1' +registries: +- url: https://workflowhub.eu + project: iwc + workflow: metaprosip/main diff --git a/workflows/proteomics/openms-metaprosip/CHANGELOG.md b/workflows/proteomics/openms-metaprosip/CHANGELOG.md new file mode 100644 index 000000000..a023e8e99 --- /dev/null +++ b/workflows/proteomics/openms-metaprosip/CHANGELOG.md @@ -0,0 +1,4 @@ +# Changelog + +## [0.1] 2023-04-13 +First release. diff --git a/workflows/proteomics/openms-metaprosip/README.md b/workflows/proteomics/openms-metaprosip/README.md new file mode 100644 index 000000000..d0bb6c82c --- /dev/null +++ b/workflows/proteomics/openms-metaprosip/README.md @@ -0,0 +1,26 @@ +# MetaProSIP: automated inference of elemental fluxes in microbial communities + +## Inputs dataset + +- `Centroided LC-MS datasets` in mzML (MetaProSIP is mainly tested on data generated by orbitrap instruments) +- `Fasta Database` in Fasta (aminoacid sequences) + +## Inputs values + +- `Precursor monoisotopic mass tolerance` (ppm): This value is passed to + - MSGFPlusAdapter parameter `Precursor monoisotopic mass tolerance` (-precursor_mass_tolerance) + - MetaProSIP parameter `Tolerance in ppm` (-mz_tolerance_ppm) +- Fixed modifications +- Variable modifications +- Labeled element + +## Processing + +- DecoyDatabase: Add decoy sequences to the Fasta database (for FDR calculation) +- FeatureFinderCentroided: identify eluting peptides that correspond to isotopologues with natural isotopic distributions +- MSGFPlusAdapter: identify peptides through peptide fragment fingerprinting (database search) +- FeatureFinderMultiplex: detect elution profiles of unlabeled peptides +- PeptideIndexer: annotate protein association to identified peptides +- FalseDiscoveryRate: Calculate FDR +- IDMapper: map identified spectra to elution profiles +- MetaProSIP: calculate the protein-SIP features, to perform functional grouping, and for protein inference diff --git a/workflows/proteomics/openms-metaprosip/metaprosip-tests.yml b/workflows/proteomics/openms-metaprosip/metaprosip-tests.yml new file mode 100644 index 000000000..d3be8fe73 --- /dev/null +++ b/workflows/proteomics/openms-metaprosip/metaprosip-tests.yml @@ -0,0 +1,39 @@ +- doc: Test outline for MetaProSIP + job: + Fasta Database: + class: File + location: https://abibuilder.cs.uni-tuebingen.de/archive/openms/galaxy-testdata/metaprosip/Zeitz_1and2_454AllContigs_HEX.fasta + filetype: fasta + Centroided LC-MS datasets: + class: Collection + collection_type: list + elements: + - class: File + identifier: Zeitz_SIP_13-II_020_picked.mzML + location: https://abibuilder.cs.uni-tuebingen.de/archive/openms/galaxy-testdata/metaprosip/Zeitz_SIP_13-II_020_picked.mzML + filetype: mzml + Precursor monoisotopic mass tolerance (ppm): 10.0 + Fixed modifications: Carbamidomethyl (C) + Variable modifications: Oxidation (M) + Labeled element: C + outputs: + Peptide centric result: + element_tests: + Zeitz_SIP_13-II_020_picked.mzML: + asserts: + - that: has_n_lines + n: 85 + - that: has_text + text: Peptide Sequence + - that: has_text + text: AATGTPSPAGSPPPIVPAPK + Feature fitting result: + element_tests: + Zeitz_SIP_13-II_020_picked.mzML: + asserts: + - that: has_n_lines + n: 4091 + - that: has_text + text: Group 1 + - that: has_text + text: LYSFHTLHQTYMK 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romane.libouban@irisa.fr diff --git a/workflows/repeatmasking/.workflowhub.yml b/workflows/repeatmasking/.workflowhub.yml new file mode 100644 index 000000000..e8c55b16c --- /dev/null +++ b/workflows/repeatmasking/.workflowhub.yml @@ -0,0 +1,5 @@ +version: '0.1' +registries: +- url: https://workflowhub.eu + project: iwc + workflow: RepeatMasking-Workflow./main diff --git a/workflows/repeatmasking/CHANGELOG.md b/workflows/repeatmasking/CHANGELOG.md new file mode 100644 index 000000000..26add980d --- /dev/null +++ b/workflows/repeatmasking/CHANGELOG.md @@ -0,0 +1,5 @@ +# Changelog + +## [0.1] + +Initial version of the RepeatMasking workflow for genomic sequencing data. \ No newline at end of file diff --git a/workflows/repeatmasking/README.md b/workflows/repeatmasking/README.md new file mode 100644 index 000000000..e17fba6a7 --- /dev/null +++ b/workflows/repeatmasking/README.md @@ -0,0 +1,29 @@ +# RepeatMasking Workflow + +This workflow uses RepeatModeler and RepeatMasker for genome analysis. + +- RepeatModeler is a software package for identifying and modeling de novo families of transposable elements (TEs). At the heart of RepeatModeler are three de novo repeat search programs (RECON, RepeatScout and LtrHarvest/Ltr_retriever) which use complementary computational methods to identify repeat element boundaries and family relationships from sequence data. + +- RepeatMasker is a program that analyzes DNA sequences for *interleaved repeats* and *low-complexity* DNA sequences. The result of the program is a detailed annotation of the repeats present in the query sequence, as well as a modified version of the query sequence in which all annotated repeats are present. + +## Input dataset for RepeatModeler +- RepeatModeler requires a single input file, a genome in fasta format. + + +## Outputs dataset for RepeatModeler +- Two output files are generated: + - summary file (.tbl) + - fasta file containing alignments in order of appearance in the query sequence + + +## Input dataset for RepeatMasker +- ReapatMasker requires the fasta file generated by RepeatModeler + +## Outputs datasets for RepeatMasker +- Five output files are generated: + - a fasta file + - .gff3 file + - a table summarizing the repeated content of the sequence analyzed + - a file with statistics related to the repeated content of the sequence analyzed + - a summary of the mutation sites found and the order of grouping + diff --git a/workflows/repeatmasking/Repeat-masking-with-RepeatModeler-and-RepeatMasker-tests.yml b/workflows/repeatmasking/Repeat-masking-with-RepeatModeler-and-RepeatMasker-tests.yml new file mode 100644 index 000000000..9e8aaecb6 --- /dev/null +++ b/workflows/repeatmasking/Repeat-masking-with-RepeatModeler-and-RepeatMasker-tests.yml @@ -0,0 +1,42 @@ +- doc: Test outline for RepeatMasking Workflow + job: + input: + class: File + location: https://zenodo.org/record/8116008/files/sequence.fasta?download=1 + filetype: fasta + + outputs: + RepeatModeler consensus sequences: + path: test-data/repeatmodeler_output_sequences.fasta + compare: sim_size + delta: 30000 + + RepeatModeler seeds alignments: + path: test-data/repeatmodeler_output_seeds.stockholm + compare: sim_size + delta: 90000000 + + RepeatMasker masked genome: + path: test-data/repeatmasker_output_masked_genome.fasta + compare: sim_size + delta: 30000 + + RepeatMasker output log: + path: test-data/repeatmasker_output_log.tabular + compare: sim_size + delta: 30000 + + RepeatMasker repeat statistics: + path: test-data/repeatmasker_output_table.txt + compare: sim_size + delta: 30000 + + RepeatMasker repeat catalog: + path: test-data/repeatmasker_output_repeat_catalog.txt + compare: sim_size + delta: 30000 + + RepeatMasker repeat annotation: + path: test-data/repeatmasker_output_gff.gff + compare: sim_size + delta: 30000 \ No newline at end of file diff --git a/workflows/repeatmasking/RepeatMasking-Workflow-tests.yml b/workflows/repeatmasking/RepeatMasking-Workflow-tests.yml new file mode 100644 index 000000000..5df56059f --- /dev/null +++ b/workflows/repeatmasking/RepeatMasking-Workflow-tests.yml @@ -0,0 +1,42 @@ +- doc: Test outline for RepeatMasking Workflow + job: + input: + class: File + location: https://zenodo.org/record/8364146/files/eco.fasta?download=1 + filetype: fasta + + outputs: + RepeatModeler consensus sequences: + location: https://zenodo.org/record/8364146/files/repeatmodeler_output_sequences.fasta?download=1 + compare: sim_size + delta: 30000 + + RepeatModeler seeds alignments: + location: https://zenodo.org/record/8364146/files/repeatmodeler_output_seeds.stockholm?download=1 + compare: sim_size + delta: 90000000 + + RepeatMasker masked genome: + location: https://zenodo.org/record/8364146/files/repeatmasker_output_masked_genome.fasta?download=1 + compare: sim_size + delta: 30000 + + RepeatMasker output log: + location: https://zenodo.org/record/8364146/files/repeatmasker_output_log.tabular?download=1 + compare: sim_size + delta: 30000 + + RepeatMasker repeat statistics: + location: https://zenodo.org/record/8364146/files/repeatmasker_output_table.txt?download=1 + compare: sim_size + delta: 30000 + + RepeatMasker repeat catalog: + location: https://zenodo.org/record/8364146/files/repeatmasker_output_repeat_catalog.txt?download=1 + compare: sim_size + delta: 30000 + + RepeatMasker repeat annotation: + location: https://zenodo.org/record/8364146/files/repeatmasker_output_gff.gff?download=1 + compare: sim_size + delta: 30000 \ No newline at end of file diff --git a/workflows/repeatmasking/RepeatMasking-Workflow.ga b/workflows/repeatmasking/RepeatMasking-Workflow.ga new file mode 100644 index 000000000..2ebbefa8a --- /dev/null +++ b/workflows/repeatmasking/RepeatMasking-Workflow.ga @@ -0,0 +1,175 @@ +{ + "a_galaxy_workflow": "true", + "annotation": "", + "format-version": "0.1", + "license": "MIT", + "release": "0.1", + "name": "Repeat masking with RepeatModeler and RepeatMasker", + "creator": [ + { + "class": "Person", + "email": "mailto:romane.libouban@irisa.fr", + "name": "Romane Libouban" + } + ], + "steps": { + "0": { + "annotation": "", + "content_id": null, + "errors": null, + "id": 0, + "input_connections": {}, + "inputs": [ + { + "description": "Apply repeat masking to this fasta file", + "name": "input" + } + ], + "label": "input", + "name": "Input dataset", + "outputs": [], + "position": { + "left": 10, + "top": 10 + }, + "tool_id": null, + "tool_state": "{\"optional\": false, \"tag\": null}", + "tool_version": null, + "type": "data_input", + "uuid": "ab5e19b0-ce35-4e54-a55e-f75243c86e3d", + "when": null, + "workflow_outputs": [] + }, + "1": { + "annotation": "", + "content_id": "toolshed.g2.bx.psu.edu/repos/csbl/repeatmodeler/repeatmodeler/2.0.4+galaxy1", + "errors": null, + "id": 1, + "input_connections": { + "input_file": { + "id": 0, + "output_name": "output" + } + }, + "inputs": [], + "label": null, + "name": "RepeatModeler", + "outputs": [ + { + "name": "sequences", + "type": "fasta" + }, + { + "name": "seeds", + "type": "stockholm" + } + ], + "position": { + "left": 230, + "top": 10 + }, + "post_job_actions": {}, + "tool_id": "toolshed.g2.bx.psu.edu/repos/csbl/repeatmodeler/repeatmodeler/2.0.4+galaxy1", + "tool_shed_repository": { + "changeset_revision": "8661b2607b7e", + "name": "repeatmodeler", + "owner": "csbl", + "tool_shed": "toolshed.g2.bx.psu.edu" + }, + "tool_state": "{\"__input_ext\": \"input\", \"chromInfo\": \"/shared/ifbstor1/galaxy/mutable-config/tool-data/shared/ucsc/chrom/?.len\", \"input_file\": null, \"__page__\": null, \"__rerun_remap_job_id__\": null}", + "tool_version": "2.0.4+galaxy1", + "type": "tool", + "uuid": "9312ba36-4275-4d40-8ba6-95eea1b23b11", + "when": null, + "workflow_outputs": [ + { + "output_name": "sequences", + "label": "RepeatModeler consensus sequences" + }, + { + "output_name": "seeds", + "label": "RepeatModeler seeds alignments" + } + ] + }, + "2": { + "annotation": "", + "content_id": "toolshed.g2.bx.psu.edu/repos/bgruening/repeat_masker/repeatmasker_wrapper/4.1.5+galaxy0", + "errors": null, + "id": 2, + "input_connections": { + "input_fasta": { + "id": 1, + "output_name": "sequences" + } + }, + "inputs": [], + "label": null, + "name": "RepeatMasker", + "outputs": [ + { + "name": "output_masked_genome", + "type": "fasta" + }, + { + "name": "output_log", + "type": "tabular" + }, + { + "name": "output_table", + "type": "txt" + }, + { + "name": "output_repeat_catalog", + "type": "txt" + }, + { + "name": "output_gff", + "type": "gff" + } + ], + "position": { + "left": 450, + "top": 10 + }, + "post_job_actions": {}, + "tool_id": "toolshed.g2.bx.psu.edu/repos/bgruening/repeat_masker/repeatmasker_wrapper/4.1.5+galaxy0", + "tool_shed_repository": { + "changeset_revision": "ba6d2c32f797", + "name": "repeat_masker", + "owner": "bgruening", + "tool_shed": "toolshed.g2.bx.psu.edu" + }, + "tool_state": "{\"__input_ext\": \"input\", \"advanced\": {\"is_only\": false, \"is_clip\": false, \"no_is\": false, \"rodspec\": false, \"primspec\": false, \"nolow\": false, \"noint\": false, \"norna\": false, \"alu\": false, \"div\": false, \"search_speed\": \"\", \"frag\": \"40000\", \"gc\": null, \"gccalc\": false, \"nocut\": false, \"xout\": false, \"keep_alignments\": false, \"invert_alignments\": false, \"poly\": false}, \"chromInfo\": \"/shared/ifbstor1/galaxy/mutable-config/tool-data/shared/ucsc/chrom/?.len\", \"excln\": true, \"gff\": true, \"input_fasta\": null, \"repeat_source\": {\"source_type\": \"dfam\", \"__current_case__\": 0, \"species_source\": {\"species_from_list\": \"no\", \"__current_case__\": 1, \"species_name\": \"\"}}, \"xsmall\": true, \"__page__\": null, \"__rerun_remap_job_id__\": null}", + "tool_version": "4.1.5+galaxy0", + "type": "tool", + "uuid": "e6c8e6a1-efe8-4291-b12b-5fdb3795b6ca", + "when": null, + "workflow_outputs": [ + { + "output_name": "output_masked_genome", + "label": "RepeatMasker masked genome" + }, + { + "output_name": "output_log", + "label": "RepeatMasker output log" + }, + { + "output_name": "output_table", + "label": "RepeatMasker repeat statistics" + }, + { + "output_name": "output_repeat_catalog", + "label": "RepeatMasker repeat catalog" + }, + { + "output_name": "output_gff", + "label": "RepeatMasker repeat annotation" + } + ] + } + }, + "tags": [], + "uuid": "f25be8fa-7823-456f-9707-a497703f48d7", + "version": 0 +} \ No newline at end of file diff --git a/workflows/requirements.txt b/workflows/requirements.txt index 6d210dd0a..fe8931a5a 100644 --- a/workflows/requirements.txt +++ b/workflows/requirements.txt @@ -1,4 +1,4 @@ rocrate~=0.4.0 planemo>=0.74.5 -pyyaml~=5.4.0 +pyyaml~=6.0.0 requests~=2.26.0 diff --git a/workflows/sars-cov-2-variant-calling/sars-cov-2-consensus-from-variation/.dockstore.yml b/workflows/sars-cov-2-variant-calling/sars-cov-2-consensus-from-variation/.dockstore.yml index 0ed316729..233b596f9 100644 --- a/workflows/sars-cov-2-variant-calling/sars-cov-2-consensus-from-variation/.dockstore.yml +++ b/workflows/sars-cov-2-variant-calling/sars-cov-2-consensus-from-variation/.dockstore.yml @@ -1,7 +1,11 @@ version: 1.2 workflows: - name: 'COVID-19-CONSENSUS-CONSTRUCTION' - primaryDescriptorPath: /consensus-from-variation.ga subclass: Galaxy + publish: true + primaryDescriptorPath: /consensus-from-variation.ga testParameterFiles: - - /consensus-from-variation-test.yml + - /consensus-from-variation-tests.yml + authors: + - name: Wolfgang Maier + orcid: 0000-0002-9464-6640 diff --git a/workflows/sars-cov-2-variant-calling/sars-cov-2-consensus-from-variation/CHANGELOG.md b/workflows/sars-cov-2-variant-calling/sars-cov-2-consensus-from-variation/CHANGELOG.md index de017e656..66826ae74 100644 --- a/workflows/sars-cov-2-variant-calling/sars-cov-2-consensus-from-variation/CHANGELOG.md +++ b/workflows/sars-cov-2-variant-calling/sars-cov-2-consensus-from-variation/CHANGELOG.md @@ -1,5 +1,15 @@ # Changelog +## [0.4.2] 2024-03-06 + +### Automatic update +- `toolshed.g2.bx.psu.edu/repos/iuc/bedtools/bedtools_genomecoveragebed/2.29.2` was updated to `toolshed.g2.bx.psu.edu/repos/iuc/bedtools/bedtools_genomecoveragebed/2.30.0` +- `toolshed.g2.bx.psu.edu/repos/iuc/bcftools_consensus/bcftools_consensus/1.15.1+galaxy2` was updated to `toolshed.g2.bx.psu.edu/repos/iuc/bcftools_consensus/bcftools_consensus/1.15.1+galaxy3` + +## [0.4.1] 2023-11-20 + +- Fix author in dockstore + ## [0.4] 2022-10-21 ### Fixed diff --git a/workflows/sars-cov-2-variant-calling/sars-cov-2-consensus-from-variation/consensus-from-variation-test.yml b/workflows/sars-cov-2-variant-calling/sars-cov-2-consensus-from-variation/consensus-from-variation-tests.yml similarity index 100% rename from workflows/sars-cov-2-variant-calling/sars-cov-2-consensus-from-variation/consensus-from-variation-test.yml rename to workflows/sars-cov-2-variant-calling/sars-cov-2-consensus-from-variation/consensus-from-variation-tests.yml diff --git a/workflows/sars-cov-2-variant-calling/sars-cov-2-consensus-from-variation/consensus-from-variation.ga b/workflows/sars-cov-2-variant-calling/sars-cov-2-consensus-from-variation/consensus-from-variation.ga index 77e948b94..7d19751ed 100644 --- a/workflows/sars-cov-2-variant-calling/sars-cov-2-consensus-from-variation/consensus-from-variation.ga +++ b/workflows/sars-cov-2-variant-calling/sars-cov-2-consensus-from-variation/consensus-from-variation.ga @@ -11,7 +11,7 @@ "format-version": "0.1", "license": "MIT", "name": "COVID-19: consensus construction", - "release": "0.4", + "release": "0.4.2", "steps": { "0": { "annotation": "Collection of VCFs produced by upstream workflows for variation analysis", @@ -29,14 +29,15 @@ "name": "Input dataset collection", "outputs": [], "position": { - "left": 348.04998779296875, - "top": 524.63330078125 + "left": 0.0, + "top": 306.13330078125 }, "tool_id": null, - "tool_state": "{\"optional\": false, \"collection_type\": \"list\"}", + "tool_state": "{\"optional\": false, \"tag\": null, \"collection_type\": \"list\"}", "tool_version": null, "type": "data_collection_input", "uuid": "fca4e710-87a7-4cb6-9ff1-f9a8dc84ca37", + "when": null, "workflow_outputs": [] }, "1": { @@ -55,14 +56,15 @@ "name": "Input parameter", "outputs": [], "position": { - "left": 348.5, - "top": 687.5 + "left": 0.45001220703125, + "top": 469.0 }, "tool_id": null, "tool_state": "{\"default\": 0.75, \"parameter_type\": \"float\", \"optional\": true}", "tool_version": null, "type": "parameter_input", "uuid": "52664bd7-b500-40a1-935b-8ac6df7003e5", + "when": null, "workflow_outputs": [ { "label": "consensus_af_threshold", @@ -87,14 +89,15 @@ "name": "Input parameter", "outputs": [], "position": { - "left": 348.5, - "top": 859.1500244140625 + "left": 0.45001220703125, + "top": 640.6500244140625 }, "tool_id": null, "tool_state": "{\"default\": 0.25, \"parameter_type\": \"float\", \"optional\": true}", "tool_version": null, "type": "parameter_input", "uuid": "a2a15cce-94ec-4a76-a3ff-3ea898e9c678", + "when": null, "workflow_outputs": [ { "label": "non_consensus_af_threshold", @@ -119,14 +122,15 @@ "name": "Input dataset collection", "outputs": [], "position": { - "left": 929.36669921875, - "top": 613.6666870117188 + "left": 581.3167114257812, + "top": 395.16668701171875 }, "tool_id": null, - "tool_state": "{\"optional\": false, \"format\": [\"bam\"], \"collection_type\": \"list\"}", + "tool_state": "{\"optional\": false, \"format\": [\"bam\"], \"tag\": null, \"collection_type\": \"list\"}", "tool_version": null, "type": "data_collection_input", "uuid": "23ae69ea-f9a7-4d5f-85f5-63b9d2bd2c50", + "when": null, "workflow_outputs": [] }, "4": { @@ -145,14 +149,15 @@ "name": "Input parameter", "outputs": [], "position": { - "left": 933.2999877929688, - "top": 776.3499755859375 + "left": 585.25, + "top": 557.8499755859375 }, "tool_id": null, "tool_state": "{\"default\": 5, \"parameter_type\": \"integer\", \"optional\": true}", "tool_version": null, "type": "parameter_input", "uuid": "cfdc65b0-8393-4ee3-9e27-16b23135fbec", + "when": null, "workflow_outputs": [ { "label": "depth_threshold", @@ -177,14 +182,15 @@ "name": "Input dataset", "outputs": [], "position": { - "left": 2631.86669921875, - "top": 564.6500244140625 + "left": 2283.8167114257812, + "top": 346.1500244140625 }, "tool_id": null, - "tool_state": "{\"optional\": false}", + "tool_state": "{\"optional\": false, \"tag\": null}", "tool_version": null, "type": "data_input", "uuid": "4a2c6df2-fb1e-42d8-96d5-6765ca85c214", + "when": null, "workflow_outputs": [] }, "6": { @@ -208,8 +214,8 @@ } ], "position": { - "left": 630.8499755859375, - "top": 503.8500061035156 + "left": 282.79998779296875, + "top": 285.3500061035156 }, "post_job_actions": { "HideDatasetActionout1": { @@ -229,6 +235,7 @@ "tool_version": "0.1.1", "type": "tool", "uuid": "8ec54933-36d6-404c-b0a6-5a14b04eb30e", + "when": null, "workflow_outputs": [] }, "7": { @@ -256,8 +263,8 @@ } ], "position": { - "left": 581.0499877929688, - "top": 1020.5166625976562 + "left": 233.0, + "top": 802.0166625976562 }, "post_job_actions": { "HideDatasetActionout1": { @@ -277,11 +284,12 @@ "tool_version": "0.1.1", "type": "tool", "uuid": 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"toolshed.g2.bx.psu.edu/repos/iuc/bedtools/bedtools_genomecoveragebed/2.30.0", "tool_shed_repository": { - "changeset_revision": "0a5c785ac6db", + "changeset_revision": "a1a923cd89e8", "name": "bedtools", "owner": "iuc", "tool_shed": "toolshed.g2.bx.psu.edu" }, - "tool_state": "{\"d\": \"false\", \"dz\": \"false\", \"five\": \"false\", \"input_type\": {\"input_type_select\": \"bam\", \"__current_case__\": 1, \"input\": {\"__class__\": \"ConnectedValue\"}}, \"report\": {\"report_select\": \"bg\", \"__current_case__\": 0, \"zero_regions\": \"true\", \"scale\": \"1.0\"}, \"split\": \"true\", \"strand\": \"\", \"three\": \"false\", \"__page__\": null, \"__rerun_remap_job_id__\": null}", - "tool_version": "2.29.2", + "tool_state": "{\"d\": false, \"dz\": false, \"five\": false, \"input_type\": {\"input_type_select\": \"bam\", \"__current_case__\": 1, \"input\": {\"__class__\": \"ConnectedValue\"}}, \"report\": {\"report_select\": \"bg\", \"__current_case__\": 0, \"zero_regions\": true, \"scale\": \"1.0\"}, \"split\": true, \"strand\": \"\", \"three\": false, \"__page__\": null, \"__rerun_remap_job_id__\": null}", + "tool_version": "2.30.0", "type": "tool", "uuid": "d89684f5-5a49-4b4c-821b-6c8a87f3a46e", + "when": null, "workflow_outputs": [ { "label": "coverage_depth", @@ -352,8 +366,8 @@ } ], "position": { - "left": 1227.36669921875, - "top": 778.3499755859375 + "left": 879.3167114257812, + "top": 559.8499755859375 }, "post_job_actions": { "HideDatasetActionout1": { @@ -373,6 +387,7 @@ "tool_version": "0.1.1", "type": "tool", "uuid": "089b2659-d4c8-44c8-9ecc-ffe71676e650", + "when": null, "workflow_outputs": [] }, "10": { @@ -390,7 +405,16 @@ "output_name": "output" } }, - "inputs": [], + "inputs": [ + { + "description": "runtime parameter for tool SnpSift Filter", + "name": "filter_expression" + }, + { + "description": "runtime parameter for tool SnpSift Filter", + "name": "input" + } + ], "label": null, "name": "SnpSift Filter", "outputs": [ @@ -400,8 +424,8 @@ } ], "position": { - "left": 833.3333129882812, - "top": 218.5 + "left": 485.2833251953125, + "top": 0.0 }, "post_job_actions": { "RenameDatasetActionoutput": { @@ -419,10 +443,11 @@ "owner": "iuc", "tool_shed": "toolshed.g2.bx.psu.edu" }, - "tool_state": "{\"filter_expression\": {\"type\": \"simple\", \"__current_case__\": 0, \"expr\": {\"__class__\": \"ConnectedValue\"}}, \"filtering\": {\"mode\": \"entries\", \"__current_case__\": 0}, \"input\": {\"__class__\": \"ConnectedValue\"}, \"inverse\": \"false\", \"__page__\": null, \"__rerun_remap_job_id__\": null}", + "tool_state": "{\"filter_expression\": {\"type\": \"simple\", \"__current_case__\": 0, \"expr\": {\"__class__\": \"ConnectedValue\"}}, \"filtering\": {\"mode\": \"entries\", \"__current_case__\": 0}, \"input\": {\"__class__\": \"ConnectedValue\"}, \"inverse\": false, \"__page__\": null, \"__rerun_remap_job_id__\": null}", "tool_version": "4.3+t.galaxy1", "type": "tool", "uuid": "3673b687-1ba0-42f2-ba13-fa3130462079", + "when": null, "workflow_outputs": [ { "label": "consensus_variants", @@ -446,7 +471,16 @@ "output_name": "output" } }, - "inputs": [], + "inputs": [ + { + "description": "runtime parameter for tool SnpSift Filter", + "name": "filter_expression" + }, + { + "description": "runtime parameter for tool SnpSift Filter", + "name": "input" + } + ], "label": null, "name": "SnpSift Filter", "outputs": [ @@ -456,8 +490,8 @@ } ], "position": { - "left": 804.7166748046875, - "top": 951.8499755859375 + "left": 456.66668701171875, + "top": 733.3499755859375 }, "post_job_actions": { "RenameDatasetActionoutput": { @@ -475,10 +509,11 @@ "owner": "iuc", "tool_shed": "toolshed.g2.bx.psu.edu" }, - "tool_state": "{\"filter_expression\": {\"type\": \"simple\", \"__current_case__\": 0, \"expr\": {\"__class__\": \"ConnectedValue\"}}, \"filtering\": {\"mode\": \"entries\", \"__current_case__\": 0}, \"input\": {\"__class__\": \"ConnectedValue\"}, \"inverse\": \"false\", \"__page__\": null, \"__rerun_remap_job_id__\": null}", + "tool_state": "{\"filter_expression\": {\"type\": \"simple\", \"__current_case__\": 0, \"expr\": {\"__class__\": \"ConnectedValue\"}}, \"filtering\": {\"mode\": \"entries\", \"__current_case__\": 0}, \"input\": {\"__class__\": \"ConnectedValue\"}, \"inverse\": false, \"__page__\": null, \"__rerun_remap_job_id__\": null}", "tool_version": "4.3+t.galaxy1", "type": "tool", "uuid": "fb232cba-e3b2-43f9-bb1f-a9ebc69c9b97", + "when": null, "workflow_outputs": [ { "label": "filter_failed_variants", @@ -502,7 +537,16 @@ "output_name": "output" } }, - "inputs": [], + "inputs": [ + { + "description": "runtime parameter for tool Filter", + "name": "cond" + }, + { + "description": "runtime parameter for tool Filter", + "name": "input" + } + ], "label": null, "name": "Filter", "outputs": [ @@ -512,8 +556,8 @@ } ], "position": { - "left": 1561.88330078125, - "top": 645.8499755859375 + "left": 1213.8333129882812, + "top": 427.3499755859375 }, "post_job_actions": { "RenameDatasetActionout_file1": { @@ -529,6 +573,7 @@ "tool_version": "1.1.1", "type": "tool", "uuid": "8ac27ee3-9e9e-4d6f-b424-2c21f80c87ef", + "when": null, "workflow_outputs": [ { "label": "low_cov_regions", @@ -548,7 +593,12 @@ "output_name": "output" } }, - "inputs": [], + "inputs": [ + { + "description": "runtime parameter for tool SnpSift Extract Fields", + "name": "input" + } + ], "label": null, "name": "SnpSift Extract Fields", "outputs": [ @@ -558,8 +608,8 @@ } ], "position": { - "left": 1155.5, - "top": 401.5 + "left": 807.4500122070312, + "top": 183.0 }, "post_job_actions": { "RenameDatasetActionoutput": { @@ -577,10 +627,11 @@ "owner": "iuc", "tool_shed": "toolshed.g2.bx.psu.edu" }, - "tool_state": "{\"empty_text\": \"\", \"extract\": \"CHROM POS REF\", \"input\": {\"__class__\": \"ConnectedValue\"}, \"one_effect_per_line\": \"false\", \"separator\": \"\", \"__page__\": null, \"__rerun_remap_job_id__\": null}", + "tool_state": "{\"empty_text\": \"\", \"extract\": \"CHROM POS REF\", \"input\": {\"__class__\": \"ConnectedValue\"}, \"one_effect_per_line\": false, \"separator\": \"\", \"__page__\": null, \"__rerun_remap_job_id__\": null}", "tool_version": "4.3+t.galaxy0", "type": "tool", "uuid": "5aca2952-a87c-4c77-a259-a22daceab557", + "when": null, "workflow_outputs": [ { "label": "chrom_pos_ref_called_variants", @@ -600,7 +651,12 @@ "output_name": "output" } }, - "inputs": [], + "inputs": [ + { + "description": "runtime parameter for tool SnpSift Extract Fields", + "name": "input" + } + ], "label": null, "name": "SnpSift Extract Fields", "outputs": [ @@ -610,8 +666,8 @@ } ], "position": { - "left": 1022, - "top": 1117.5 + "left": 673.9500122070312, + "top": 899.0 }, "post_job_actions": { "RenameDatasetActionoutput": { @@ -629,10 +685,11 @@ "owner": "iuc", "tool_shed": "toolshed.g2.bx.psu.edu" }, - "tool_state": "{\"empty_text\": \"\", \"extract\": \"CHROM POS REF\", \"input\": {\"__class__\": \"ConnectedValue\"}, \"one_effect_per_line\": \"false\", \"separator\": \"\", \"__page__\": null, \"__rerun_remap_job_id__\": null}", + "tool_state": "{\"empty_text\": \"\", \"extract\": \"CHROM POS REF\", \"input\": {\"__class__\": \"ConnectedValue\"}, \"one_effect_per_line\": false, \"separator\": \"\", \"__page__\": null, \"__rerun_remap_job_id__\": null}", "tool_version": "4.3+t.galaxy0", "type": "tool", "uuid": "d99d1a1a-24a7-4379-a7d1-1980303ee08a", + "when": null, "workflow_outputs": [ { "label": "chrom_pos_ref_failed_variants", @@ -667,8 +724,8 @@ } ], "position": { - "left": 1496.183349609375, - "top": 468.1499938964844 + "left": 1148.1333618164062, + "top": 249.64999389648438 }, "post_job_actions": { "ChangeDatatypeActionout_file1": { @@ -693,10 +750,11 @@ "owner": "devteam", "tool_shed": "toolshed.g2.bx.psu.edu" }, - "tool_state": "{\"avoid_scientific_notation\": \"false\", \"error_handling\": {\"auto_col_types\": \"false\", \"fail_on_non_existent_columns\": \"true\", \"non_computable\": {\"action\": \"--skip-non-computable\", \"__current_case__\": 1}}, \"input\": {\"__class__\": \"RuntimeValue\"}, \"ops\": {\"header_lines_select\": \"no\", \"__current_case__\": 0, \"expressions\": [{\"__index__\": 0, \"cond\": \"int(c2) - (len(c3) == 1)\", \"add_column\": {\"mode\": \"R\", \"__current_case__\": 2, \"pos\": \"2\"}}, {\"__index__\": 1, \"cond\": \"int(c2) + ((len(c3) - 1) or 1)\", \"add_column\": {\"mode\": \"R\", \"__current_case__\": 2, \"pos\": \"3\"}}]}, \"__page__\": null, \"__rerun_remap_job_id__\": null}", + "tool_state": "{\"avoid_scientific_notation\": false, \"error_handling\": {\"auto_col_types\": false, \"fail_on_non_existent_columns\": true, \"non_computable\": {\"action\": \"--skip-non-computable\", \"__current_case__\": 1}}, \"input\": {\"__class__\": \"ConnectedValue\"}, \"ops\": {\"header_lines_select\": \"no\", \"__current_case__\": 0, \"expressions\": [{\"__index__\": 0, \"cond\": \"int(c2) - (len(c3) == 1)\", \"add_column\": {\"mode\": \"R\", \"__current_case__\": 2, \"pos\": \"2\"}}, {\"__index__\": 1, \"cond\": \"int(c2) + ((len(c3) - 1) or 1)\", \"add_column\": {\"mode\": \"R\", \"__current_case__\": 2, \"pos\": \"3\"}}]}, \"__page__\": null, \"__rerun_remap_job_id__\": null}", "tool_version": "2.0", "type": "tool", "uuid": "fd260e32-723d-4a22-8d64-4795557f8159", + "when": null, "workflow_outputs": [ { "label": "called_variant_sites", @@ -731,8 +789,8 @@ } ], "position": { - "left": 1249.6666259765625, - "top": 1137.6500244140625 + "left": 901.6166381835938, + "top": 919.1500244140625 }, "post_job_actions": { "ChangeDatatypeActionout_file1": { @@ -757,10 +815,11 @@ "owner": "devteam", "tool_shed": "toolshed.g2.bx.psu.edu" }, - "tool_state": "{\"avoid_scientific_notation\": \"false\", \"error_handling\": {\"auto_col_types\": \"false\", \"fail_on_non_existent_columns\": \"true\", \"non_computable\": {\"action\": \"--skip-non-computable\", \"__current_case__\": 1}}, \"input\": {\"__class__\": \"RuntimeValue\"}, \"ops\": {\"header_lines_select\": \"no\", \"__current_case__\": 0, \"expressions\": [{\"__index__\": 0, \"cond\": \"int(c2) - (len(c3) == 1)\", \"add_column\": {\"mode\": \"R\", \"__current_case__\": 2, \"pos\": \"2\"}}, {\"__index__\": 1, \"cond\": \"int(c2) + ((len(c3) - 1) or 1)\", \"add_column\": {\"mode\": \"R\", \"__current_case__\": 2, \"pos\": \"3\"}}]}, \"__page__\": null, \"__rerun_remap_job_id__\": null}", + "tool_state": "{\"avoid_scientific_notation\": false, \"error_handling\": {\"auto_col_types\": false, \"fail_on_non_existent_columns\": true, \"non_computable\": {\"action\": \"--skip-non-computable\", \"__current_case__\": 1}}, \"input\": {\"__class__\": \"ConnectedValue\"}, \"ops\": {\"header_lines_select\": \"no\", \"__current_case__\": 0, \"expressions\": [{\"__index__\": 0, \"cond\": \"int(c2) - (len(c3) == 1)\", \"add_column\": {\"mode\": \"R\", \"__current_case__\": 2, \"pos\": \"2\"}}, {\"__index__\": 1, \"cond\": \"int(c2) + ((len(c3) - 1) or 1)\", \"add_column\": {\"mode\": \"R\", \"__current_case__\": 2, \"pos\": \"3\"}}]}, \"__page__\": null, \"__rerun_remap_job_id__\": null}", "tool_version": "2.0", "type": "tool", "uuid": "116e8e99-53ca-4366-a7e1-e3a9fb0bcfc3", + "when": null, "workflow_outputs": [ { "label": "failed_variant_sites", @@ -784,7 +843,16 @@ "output_name": "out_file1" } }, - "inputs": [], + "inputs": [ + { + "description": "runtime parameter for tool Concatenate", + "name": "input1" + }, + { + "description": "runtime parameter for tool Concatenate", + "name": "input2" + } + ], "label": null, "name": "Concatenate", "outputs": [ @@ -794,8 +862,8 @@ } ], "position": { - "left": 1816.933349609375, - "top": 755.9166870117188 + "left": 1468.8833618164062, + "top": 537.4166870117188 }, "post_job_actions": { "RenameDatasetActionoutput": { @@ -808,15 +876,16 @@ }, "tool_id": "toolshed.g2.bx.psu.edu/repos/devteam/concat/gops_concat_1/1.0.1", "tool_shed_repository": { - "changeset_revision": "35a89f8cc96e", + "changeset_revision": "73ca13a7ec5f", "name": "concat", "owner": "devteam", "tool_shed": "toolshed.g2.bx.psu.edu" }, - "tool_state": "{\"input1\": {\"__class__\": \"ConnectedValue\"}, \"input2\": {\"__class__\": \"ConnectedValue\"}, \"sameformat\": \"false\", \"__page__\": null, \"__rerun_remap_job_id__\": null}", + "tool_state": "{\"input1\": {\"__class__\": \"ConnectedValue\"}, \"input2\": {\"__class__\": \"ConnectedValue\"}, \"sameformat\": false, \"__page__\": null, \"__rerun_remap_job_id__\": null}", "tool_version": "1.0.1", "type": "tool", "uuid": "2b7570fb-9ce5-4a9a-863c-e33efbc88912", + "when": null, "workflow_outputs": [ { "label": "low_cov_regions_plus_filter_failed", @@ -836,7 +905,12 @@ "output_name": "output" } }, - "inputs": [], + "inputs": [ + { + "description": "runtime parameter for tool Merge", + "name": "input1" + } + ], "label": null, "name": "Merge", "outputs": [ @@ -846,8 +920,8 @@ } ], "position": { - "left": 2051.36669921875, - "top": 752.1666870117188 + "left": 1703.3167114257812, + "top": 533.6666870117188 }, "post_job_actions": { "ChangeDatatypeActionoutput": { @@ -867,15 +941,16 @@ }, "tool_id": "toolshed.g2.bx.psu.edu/repos/devteam/merge/gops_merge_1/1.0.0", "tool_shed_repository": { - "changeset_revision": "381cd27bf67a", + "changeset_revision": "debffd27642d", "name": "merge", "owner": "devteam", "tool_shed": "toolshed.g2.bx.psu.edu" }, - "tool_state": "{\"input1\": {\"__class__\": \"ConnectedValue\"}, \"returntype\": \"true\", \"__page__\": null, \"__rerun_remap_job_id__\": null}", + "tool_state": "{\"input1\": {\"__class__\": \"ConnectedValue\"}, \"returntype\": true, \"__page__\": null, \"__rerun_remap_job_id__\": null}", "tool_version": "1.0.0", "type": "tool", "uuid": "3e63167b-6560-4b6d-a409-7d3707039222", + "when": null, "workflow_outputs": [ { "label": "low_cov_regions_plus_filter_failed_combined", @@ -899,7 +974,16 @@ "output_name": "out_file1" } }, - "inputs": [], + "inputs": [ + { + "description": "runtime parameter for tool Subtract", + "name": "input1" + }, + { + "description": "runtime parameter for tool Subtract", + "name": "input2" + } + ], "label": null, "name": "Subtract", "outputs": [ @@ -909,8 +993,8 @@ } ], "position": { - "left": 2307.5166015625, - "top": 626.5 + "left": 1959.4666137695312, + "top": 408.0 }, "post_job_actions": { "RenameDatasetActionoutput": { @@ -923,7 +1007,7 @@ }, "tool_id": "toolshed.g2.bx.psu.edu/repos/devteam/subtract/gops_subtract_1/1.0.0", "tool_shed_repository": { - "changeset_revision": "0145969324c4", + "changeset_revision": "0427ca314f3d", "name": "subtract", "owner": "devteam", "tool_shed": "toolshed.g2.bx.psu.edu" @@ -932,6 +1016,7 @@ "tool_version": "1.0.0", "type": "tool", "uuid": "51b26960-4ee7-4a3d-96cb-a6b3950c173e", + "when": null, "workflow_outputs": [ { "label": "masking_regions", @@ -966,8 +1051,8 @@ } ], "position": { - "left": 2492.88330078125, - "top": 804.1666870117188 + "left": 2144.8333129882812, + "top": 585.6666870117188 }, "post_job_actions": { "ChangeDatatypeActionout_file1": { @@ -992,10 +1077,11 @@ "owner": "devteam", "tool_shed": "toolshed.g2.bx.psu.edu" }, - "tool_state": "{\"avoid_scientific_notation\": \"false\", \"error_handling\": {\"auto_col_types\": \"true\", \"fail_on_non_existent_columns\": \"true\", \"non_computable\": {\"action\": \"--fail-on-non-computable\", \"__current_case__\": 0}}, \"input\": {\"__class__\": \"RuntimeValue\"}, \"ops\": {\"header_lines_select\": \"no\", \"__current_case__\": 0, \"expressions\": [{\"__index__\": 0, \"cond\": \"int(c2) + 1\", \"add_column\": {\"mode\": \"R\", \"__current_case__\": 2, \"pos\": \"2\"}}]}, \"__page__\": null, \"__rerun_remap_job_id__\": null}", + "tool_state": "{\"avoid_scientific_notation\": false, \"error_handling\": {\"auto_col_types\": true, \"fail_on_non_existent_columns\": true, \"non_computable\": {\"action\": \"--fail-on-non-computable\", \"__current_case__\": 0}}, \"input\": {\"__class__\": \"ConnectedValue\"}, \"ops\": {\"header_lines_select\": \"no\", \"__current_case__\": 0, \"expressions\": [{\"__index__\": 0, \"cond\": \"int(c2) + 1\", \"add_column\": {\"mode\": \"R\", \"__current_case__\": 2, \"pos\": \"2\"}}]}, \"__page__\": null, \"__rerun_remap_job_id__\": null}", "tool_version": "2.0", "type": "tool", "uuid": "e06fe045-f0f0-4c32-80ad-a8e070946a2f", + "when": null, "workflow_outputs": [ { "label": "1_based_masking_regions", @@ -1006,7 +1092,7 @@ }, "21": { "annotation": "", - "content_id": "toolshed.g2.bx.psu.edu/repos/iuc/bcftools_consensus/bcftools_consensus/1.15.1+galaxy2", + "content_id": "toolshed.g2.bx.psu.edu/repos/iuc/bcftools_consensus/bcftools_consensus/1.15.1+galaxy3", "errors": null, "id": 21, "input_connections": { @@ -1023,7 +1109,20 @@ "output_name": "out_file1" } }, - "inputs": [], + "inputs": [ + { + "description": "runtime parameter for tool bcftools consensus", + "name": "input_file" + }, + { + "description": "runtime parameter for tool bcftools consensus", + "name": "reference_source" + }, + { + "description": "runtime parameter for tool bcftools consensus", + "name": "sec_default" + } + ], "label": null, "name": "bcftools consensus", "outputs": [ @@ -1033,8 +1132,8 @@ } ], "position": { - "left": 2951.88330078125, - "top": 462.16668701171875 + "left": 2603.8333129882812, + "top": 243.66668701171875 }, "post_job_actions": { "RenameDatasetActionoutput_file": { @@ -1045,17 +1144,18 @@ "output_name": "output_file" } }, - "tool_id": "toolshed.g2.bx.psu.edu/repos/iuc/bcftools_consensus/bcftools_consensus/1.15.1+galaxy2", + "tool_id": "toolshed.g2.bx.psu.edu/repos/iuc/bcftools_consensus/bcftools_consensus/1.15.1+galaxy3", "tool_shed_repository": { - "changeset_revision": "d3081540d175", + "changeset_revision": "147de996e34f", "name": "bcftools_consensus", "owner": "iuc", "tool_shed": "toolshed.g2.bx.psu.edu" }, - "tool_state": "{\"absent\": \"\", \"chain\": \"false\", \"input_file\": {\"__class__\": \"RuntimeValue\"}, \"reference_source\": {\"reference_source_selector\": \"history\", \"__current_case__\": 1, \"fasta_ref\": {\"__class__\": \"RuntimeValue\"}}, \"rename\": \"true\", \"sec_default\": {\"mask\": {\"__class__\": \"RuntimeValue\"}, \"iupac_codes\": \"false\", \"sample\": \"\", \"select_haplotype\": null, \"mark_del\": \"\", \"mark_ins\": null, \"mark_snv\": null, \"conditional_mask\": {\"selector\": \"disabled\", \"__current_case__\": 0}}, \"sec_restrict\": {\"include\": \"\", \"exclude\": \"\"}, \"__page__\": null, \"__rerun_remap_job_id__\": null}", - "tool_version": "1.15.1+galaxy2", + "tool_state": "{\"absent\": \"\", \"chain\": false, \"input_file\": {\"__class__\": \"ConnectedValue\"}, \"reference_source\": {\"reference_source_selector\": \"history\", \"__current_case__\": 1, \"fasta_ref\": {\"__class__\": \"ConnectedValue\"}}, \"rename\": true, \"sec_default\": {\"mask\": {\"__class__\": \"ConnectedValue\"}, \"iupac_codes\": false, \"sample\": \"\", \"select_haplotype\": null, \"mark_del\": \"\", \"mark_ins\": null, \"mark_snv\": null, \"conditional_mask\": {\"selector\": \"disabled\", \"__current_case__\": 0}}, \"sec_restrict\": {\"include\": \"\", \"exclude\": \"\"}, \"__page__\": null, \"__rerun_remap_job_id__\": null}", + "tool_version": "1.15.1+galaxy3", "type": "tool", "uuid": "698a9a8c-f6fe-430b-a7e0-cf9e1d058d02", + "when": null, "workflow_outputs": [ { "label": "consensus", @@ -1075,7 +1175,12 @@ "output_name": "output_file" } }, - "inputs": [], + "inputs": [ + { + "description": "runtime parameter for tool Collapse Collection", + "name": "input_list" + } + ], "label": null, "name": "Collapse Collection", "outputs": [ @@ -1085,8 +1190,8 @@ } ], "position": { - "left": 3211.550048828125, - "top": 486 + "left": 2863.5000610351562, + "top": 267.5 }, "post_job_actions": { "RenameDatasetActionoutput": { @@ -1104,10 +1209,11 @@ "owner": "nml", "tool_shed": "toolshed.g2.bx.psu.edu" }, - "tool_state": "{\"filename\": {\"add_name\": \"false\", \"__current_case__\": 1}, \"input_list\": {\"__class__\": \"ConnectedValue\"}, \"one_header\": \"false\", \"__page__\": null, \"__rerun_remap_job_id__\": null}", + "tool_state": "{\"filename\": {\"add_name\": false, \"__current_case__\": 1}, \"input_list\": {\"__class__\": \"ConnectedValue\"}, \"one_header\": false, \"__page__\": null, \"__rerun_remap_job_id__\": null}", "tool_version": "5.1.0", "type": "tool", "uuid": "acae0f3e-448b-483e-a44a-3e09ce9b3e77", + "when": null, "workflow_outputs": [ { "label": "multisample_consensus_fasta", @@ -1122,4 +1228,4 @@ "covid19.galaxyproject.org" ], "uuid": "06dc40a7-99f2-4b3c-ae21-b3dcb239306f" -} +} \ No newline at end of file diff --git a/workflows/sars-cov-2-variant-calling/sars-cov-2-ont-artic-variant-calling/.dockstore.yml b/workflows/sars-cov-2-variant-calling/sars-cov-2-ont-artic-variant-calling/.dockstore.yml index c38c6b8b5..e3a5c681c 100644 --- a/workflows/sars-cov-2-variant-calling/sars-cov-2-ont-artic-variant-calling/.dockstore.yml +++ b/workflows/sars-cov-2-variant-calling/sars-cov-2-ont-artic-variant-calling/.dockstore.yml @@ -1,7 +1,11 @@ version: 1.2 workflows: - name: 'COVID-19-ARTIC-ONT' - primaryDescriptorPath: /ont-artic-variation.ga subclass: Galaxy + publish: true + primaryDescriptorPath: /ont-artic-variation.ga testParameterFiles: - - /ont-artic-variation-test.yml + - /ont-artic-variation-tests.yml + authors: + - name: Wolfgang Maier + orcid: 0000-0002-9464-6640 diff --git a/workflows/sars-cov-2-variant-calling/sars-cov-2-ont-artic-variant-calling/CHANGELOG.md b/workflows/sars-cov-2-variant-calling/sars-cov-2-ont-artic-variant-calling/CHANGELOG.md index 4059e2a8c..4df63ab52 100644 --- a/workflows/sars-cov-2-variant-calling/sars-cov-2-ont-artic-variant-calling/CHANGELOG.md +++ b/workflows/sars-cov-2-variant-calling/sars-cov-2-ont-artic-variant-calling/CHANGELOG.md @@ -1,5 +1,10 @@ # Changelog +## [0.3.2] 2023-11-20 + +- Fix author in dockstore +- Use ncbi link instead of googleapis for fastq + ## [0.3.1] 2021-12-13 ### Added diff --git a/workflows/sars-cov-2-variant-calling/sars-cov-2-ont-artic-variant-calling/ont-artic-variation-test.yml b/workflows/sars-cov-2-variant-calling/sars-cov-2-ont-artic-variant-calling/ont-artic-variation-tests.yml similarity index 85% rename from workflows/sars-cov-2-variant-calling/sars-cov-2-ont-artic-variant-calling/ont-artic-variation-test.yml rename to workflows/sars-cov-2-variant-calling/sars-cov-2-ont-artic-variant-calling/ont-artic-variation-tests.yml index 469a5a874..0d00e32e1 100644 --- a/workflows/sars-cov-2-variant-calling/sars-cov-2-ont-artic-variant-calling/ont-artic-variation-test.yml +++ b/workflows/sars-cov-2-variant-calling/sars-cov-2-ont-artic-variant-calling/ont-artic-variation-tests.yml @@ -13,7 +13,7 @@ - identifier: SRR12447380 class: File filetype: fastqsanger.gz - location: "https://storage.googleapis.com/nih-sequence-read-archive/sra-src/SRR12447380/MDHP-00017_NB09.covfiltered.fq.gz.1" + location: "https://www.be-md.ncbi.nlm.nih.gov/Traces/sra-reads-be/fastq?acc=SRR12447380" outputs: annotated_softfiltered_variants: attributes: {} diff --git a/workflows/sars-cov-2-variant-calling/sars-cov-2-ont-artic-variant-calling/ont-artic-variation.ga b/workflows/sars-cov-2-variant-calling/sars-cov-2-ont-artic-variant-calling/ont-artic-variation.ga index d958148d5..bb74a46bd 100644 --- a/workflows/sars-cov-2-variant-calling/sars-cov-2-ont-artic-variant-calling/ont-artic-variation.ga +++ b/workflows/sars-cov-2-variant-calling/sars-cov-2-ont-artic-variant-calling/ont-artic-variation.ga @@ -11,7 +11,7 @@ "format-version": "0.1", "license": "MIT", "name": "COVID-19: variation analysis of ARTIC ONT data", - "release": "0.3.1", + "release": "0.3.2", "steps": { "0": { "annotation": "ONT reads from ARTIC assay with fastqsanger encoding", diff --git a/workflows/sars-cov-2-variant-calling/sars-cov-2-pe-illumina-artic-ivar-analysis/.dockstore.yml b/workflows/sars-cov-2-variant-calling/sars-cov-2-pe-illumina-artic-ivar-analysis/.dockstore.yml index 6986ad2a0..b74776f2c 100644 --- a/workflows/sars-cov-2-variant-calling/sars-cov-2-pe-illumina-artic-ivar-analysis/.dockstore.yml +++ b/workflows/sars-cov-2-variant-calling/sars-cov-2-pe-illumina-artic-ivar-analysis/.dockstore.yml @@ -1,7 +1,9 @@ version: 1.2 workflows: - name: 'SARS-COV-2-ILLUMINA-AMPLICON-IVAR-PANGOLIN-NEXTCLADE' - primaryDescriptorPath: /pe-wgs-ivar-analysis.ga subclass: Galaxy - testParameterFiles: - - /pe-wgs-ivar-analysis-test.yml + publish: true + primaryDescriptorPath: /pe-wgs-ivar-analysis.ga + authors: + - name: Peter van Heusden + orcid: 0000-0001-6553-5274 diff --git a/workflows/sars-cov-2-variant-calling/sars-cov-2-pe-illumina-artic-ivar-analysis/CHANGELOG.md b/workflows/sars-cov-2-variant-calling/sars-cov-2-pe-illumina-artic-ivar-analysis/CHANGELOG.md index 5e1eab082..ef8130c80 100644 --- a/workflows/sars-cov-2-variant-calling/sars-cov-2-pe-illumina-artic-ivar-analysis/CHANGELOG.md +++ b/workflows/sars-cov-2-variant-calling/sars-cov-2-pe-illumina-artic-ivar-analysis/CHANGELOG.md @@ -1,5 +1,19 @@ # Changelog +## [0.3] 2022-11-22 + +- update all tools: + - fastp from 0.20.1 to 0.23.2 + - bwa_mem from 0.7.17.1 to 0.7.17.2 + - samtools_stats from 2.0.2 to 2.0.4 + - samtools_view from 1.9 to 1.15.1 + - ivar_trim from 1.3.1 to 1.4.2 + - ivar_variants from 1.3.1 to 1.4.2 + - ivar_consensus from 1.3.1 to 1.4.2 + - multiqc from 1.9 to 1.11 + - pangolin from 3.1.14 to 4.3 + - nextclade from 1.4.1 to 2.7.0 + ## [0.2.3] 2022-05-25 ### Changed diff --git a/workflows/sars-cov-2-variant-calling/sars-cov-2-pe-illumina-artic-ivar-analysis/pe-wgs-ivar-analysis-test.yml b/workflows/sars-cov-2-variant-calling/sars-cov-2-pe-illumina-artic-ivar-analysis/pe-wgs-ivar-analysis-test.yml index 784a4751e..8d48cd411 100644 --- a/workflows/sars-cov-2-variant-calling/sars-cov-2-pe-illumina-artic-ivar-analysis/pe-wgs-ivar-analysis-test.yml +++ b/workflows/sars-cov-2-variant-calling/sars-cov-2-pe-illumina-artic-ivar-analysis/pe-wgs-ivar-analysis-test.yml @@ -28,13 +28,13 @@ all_samples_nextclade: asserts: has_text: - text: '20B 0' + text: '20B 1' has_text_matching: - expression: "seqName\tclade\tqc.overallScore\tqc.overallStatus\ttotalSubstitutions\ttotalDeletions\ttotalInsertions\ttotalFrameShifts\ttotalAminoacidSubstitutions\ttotalAminoacidDeletions\ttotalMissing\ttotalNonACGTNs\ttotalPcrPrimerChanges\tsubstitutions\tdeletions\tinsertions\tframeShifts\taaSubstitutions\taaDeletions\tmissing\tnonACGTNs\tpcrPrimerChanges\talignmentScore\talignmentStart\talignmentEnd\tqc.missingData.missingDataThreshold\tqc.missingData.score\tqc.missingData.status\tqc.missingData.totalMissing\tqc.mixedSites.mixedSitesThreshold\tqc.mixedSites.score\tqc.mixedSites.status\tqc.mixedSites.totalMixedSites\tqc.privateMutations.cutoff\tqc.privateMutations.excess\tqc.privateMutations.score\tqc.privateMutations.status\tqc.privateMutations.total\tqc.snpClusters.clusteredSNPs\tqc.snpClusters.score\tqc.snpClusters.status\tqc.snpClusters.totalSNPs\tqc.frameShifts.frameShifts\tqc.frameShifts.totalFrameShifts\tqc.frameShifts.frameShiftsIgnored\tqc.frameShifts.totalFrameShiftsIgnored\tqc.frameShifts.score\tqc.frameShifts.status\tqc.stopCodons.stopCodons\tqc.stopCodons.totalStopCodons\tqc.stopCodons.score\tqc.stopCodons.status\terrors" + expression: "seqName\tclade\tNextclade_pango\tpartiallyAliased\tclade_nextstrain\tclade_who\tclade_display\tqc.overallScore\tqc.overallStatus\ttotalSubstitutions\ttotalDeletions\ttotalInsertions\ttotalFrameShifts\ttotalAminoacidSubstitutions\ttotalAminoacidDeletions\ttotalAminoacidInsertions\ttotalMissing\ttotalNonACGTNs\ttotalPcrPrimerChanges\tsubstitutions\tdeletions\tinsertions\tprivateNucMutations.reversionSubstitutions\tprivateNucMutations.labeledSubstitutions\tprivateNucMutations.unlabeledSubstitutions\tprivateNucMutations.totalReversionSubstitutions\tprivateNucMutations.totalLabeledSubstitutions\tprivateNucMutations.totalUnlabeledSubstitutions\tprivateNucMutations.totalPrivateSubstitutions\tframeShifts\taaSubstitutions\taaDeletions\taaInsertions\tmissing\tnonACGTNs\tpcrPrimerChanges\talignmentScore\talignmentStart\talignmentEnd\tcoverage\tqc.missingData.missingDataThreshold\tqc.missingData.score\tqc.missingData.status\tqc.missingData.totalMissing\tqc.mixedSites.mixedSitesThreshold\tqc.mixedSites.score\tqc.mixedSites.status\tqc.mixedSites.totalMixedSites\tqc.privateMutations.cutoff\tqc.privateMutations.excess\tqc.privateMutations.score\tqc.privateMutations.status\tqc.privateMutations.total\tqc.snpClusters.clusteredSNPs\tqc.snpClusters.score\tqc.snpClusters.status\tqc.snpClusters.totalSNPs\tqc.frameShifts.frameShifts\tqc.frameShifts.totalFrameShifts\tqc.frameShifts.frameShiftsIgnored\tqc.frameShifts.totalFrameShiftsIgnored\tqc.frameShifts.score\tqc.frameShifts.status\tqc.stopCodons.stopCodons\tqc.stopCodons.totalStopCodons\tqc.stopCodons.score\tqc.stopCodons.status\tisReverseComplement\tfailedGenes\twarnings\terrors" all_samples_pangolin: asserts: has_text_matching: - expression: "taxon\tlineage\tconflict\tambiguity_score\tscorpio_call\tscorpio_support\tscorpio_conflict\tversion\tpangolin_version\tpangoLEARN_version\tpango_version\tstatus\tnote" + expression: "taxon\tlineage\tconflict\tambiguity_score\tscorpio_call\tscorpio_support\tscorpio_conflict\tscorpio_notes\tversion\tpangolin_version\tscorpio_version\tconstellation_version\tis_designated\tqc_status\tqc_notes\tnote" combined_multifasta: asserts: has_line: diff --git a/workflows/sars-cov-2-variant-calling/sars-cov-2-pe-illumina-artic-ivar-analysis/pe-wgs-ivar-analysis.ga b/workflows/sars-cov-2-variant-calling/sars-cov-2-pe-illumina-artic-ivar-analysis/pe-wgs-ivar-analysis.ga index eaad1c6a8..01729ebac 100644 --- a/workflows/sars-cov-2-variant-calling/sars-cov-2-pe-illumina-artic-ivar-analysis/pe-wgs-ivar-analysis.ga +++ b/workflows/sars-cov-2-variant-calling/sars-cov-2-pe-illumina-artic-ivar-analysis/pe-wgs-ivar-analysis.ga @@ -8,7 +8,7 @@ "name": "Peter van Heusden" } ], - "release": "0.2.3", +"release": "0.2.3", "format-version": "0.1", "license": "MIT", "name": "SARS-CoV-2 Illumina Amplicon pipeline - iVar based", @@ -29,20 +29,15 @@ "name": "Input dataset collection", "outputs": [], "position": { - "bottom": 338.74998474121094, - "height": 82.19999694824219, - "left": -364.683349609375, - "right": -164.683349609375, - "top": 256.54998779296875, - "width": 200, - "x": -364.683349609375, - "y": 256.54998779296875 + "left": 2, + "top": 33.99998474121094 }, "tool_id": null, - "tool_state": "{\"optional\": false, \"collection_type\": \"list:paired\"}", + "tool_state": "{\"optional\": false, \"tag\": null, \"collection_type\": \"list:paired\"}", "tool_version": null, "type": "data_collection_input", "uuid": "ce39f6b7-b9f6-4431-8831-7a284fe826a7", + "when": null, "workflow_outputs": [] }, "1": { @@ -61,20 +56,15 @@ "name": "Input dataset", "outputs": [], "position": { - "bottom": 564.3499908447266, - "height": 61.80000305175781, - "left": -366.683349609375, - "right": -166.683349609375, - "top": 502.54998779296875, - "width": 200, - "x": -366.683349609375, - "y": 502.54998779296875 + "left": 0, + "top": 279.99998474121094 }, "tool_id": null, - "tool_state": "{\"optional\": false}", + "tool_state": "{\"optional\": false, \"tag\": null}", "tool_version": null, "type": "data_input", "uuid": "25167e70-195c-4cda-9219-dbfe2f70c863", + "when": null, "workflow_outputs": [] }, "2": { @@ -93,20 +83,15 @@ "name": "Input dataset", "outputs": [], "position": { - "bottom": 696.2499847412109, - "height": 82.19999694824219, - "left": -360.683349609375, - "right": -160.683349609375, - "top": 614.0499877929688, - "width": 200, - "x": -360.683349609375, - "y": 614.0499877929688 + "left": 6, + "top": 391.49998474121094 }, "tool_id": null, - "tool_state": "{\"optional\": false}", + "tool_state": "{\"optional\": false, \"tag\": null}", "tool_version": null, "type": "data_input", "uuid": "6c8951a5-f254-4564-8d17-f78e018077ec", + "when": null, "workflow_outputs": [] }, "3": { @@ -125,20 +110,15 @@ "name": "Input parameter", "outputs": [], "position": { - "bottom": 961.1499786376953, - "height": 102.59999084472656, - "left": -332.33331298828125, - "right": -132.33331298828125, - "top": 858.5499877929688, - "width": 200, - "x": -332.33331298828125, - "y": 858.5499877929688 + "left": 34.35003662109375, + "top": 635.9999847412109 }, "tool_id": null, "tool_state": "{\"default\": 0.7, \"parameter_type\": \"float\", \"optional\": true}", "tool_version": null, "type": "parameter_input", "uuid": "57a3622f-6908-4c1d-b88d-3bc318ac9d73", + "when": null, "workflow_outputs": [] }, "4": { @@ -157,25 +137,20 @@ "name": "Input parameter", "outputs": [], "position": { - "bottom": 1127.1500396728516, - "height": 102.59999084472656, - "left": -337.2833251953125, - "right": -137.2833251953125, - "top": 1024.550048828125, - "width": 200, - "x": -337.2833251953125, - "y": 1024.550048828125 + "left": 29.4000244140625, + "top": 802.0000457763672 }, "tool_id": null, "tool_state": "{\"default\": 20, \"parameter_type\": \"integer\", \"optional\": true}", "tool_version": null, "type": "parameter_input", "uuid": "00fdc318-b4b7-404e-900b-54087a891f55", + "when": null, "workflow_outputs": [] }, "5": { "annotation": "", - "content_id": "toolshed.g2.bx.psu.edu/repos/iuc/fastp/fastp/0.20.1+galaxy0", + "content_id": "toolshed.g2.bx.psu.edu/repos/iuc/fastp/fastp/0.23.2+galaxy0", "errors": null, "id": 5, "input_connections": { @@ -202,14 +177,8 @@ } ], "position": { - "bottom": 458.1499938964844, - "height": 235.59999084472656, - "left": -86.68333435058594, - "right": 113.31666564941406, - "top": 222.5500030517578, - "width": 200, - "x": -86.68333435058594, - "y": 222.5500030517578 + "left": 280.00001525878906, + "top": 0 }, "post_job_actions": { "HideDatasetActionoutput_paired_coll": { @@ -228,17 +197,18 @@ "output_name": "report_json" } }, - "tool_id": "toolshed.g2.bx.psu.edu/repos/iuc/fastp/fastp/0.20.1+galaxy0", + "tool_id": "toolshed.g2.bx.psu.edu/repos/iuc/fastp/fastp/0.23.2+galaxy0", "tool_shed_repository": { - "changeset_revision": "dbf9c561ef29", + "changeset_revision": "65b93b623c77", "name": "fastp", "owner": "iuc", "tool_shed": "toolshed.g2.bx.psu.edu" }, - "tool_state": "{\"filter_options\": {\"quality_filtering_options\": {\"disable_quality_filtering\": \"false\", \"qualified_quality_phred\": null, \"unqualified_percent_limit\": null, \"n_base_limit\": null}, \"length_filtering_options\": {\"disable_length_filtering\": \"false\", \"length_required\": null, \"length_limit\": null}, \"low_complexity_filter\": {\"enable_low_complexity_filter\": \"false\", \"complexity_threshold\": null}}, \"output_options\": {\"report_html\": \"true\", \"report_json\": \"true\"}, \"overrepresented_sequence_analysis\": {\"overrepresentation_analysis\": \"false\", \"overrepresentation_sampling\": null}, \"read_mod_options\": 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+ "tool_state": "{\"filter_options\": {\"quality_filtering_options\": {\"disable_quality_filtering\": false, \"qualified_quality_phred\": null, \"unqualified_percent_limit\": null, \"n_base_limit\": null}, \"length_filtering_options\": {\"disable_length_filtering\": false, \"length_required\": null, \"length_limit\": null}, \"low_complexity_filter\": {\"enable_low_complexity_filter\": false, \"complexity_threshold\": null}}, \"output_options\": {\"report_html\": true, \"report_json\": true}, \"overrepresented_sequence_analysis\": {\"overrepresentation_analysis\": false, \"overrepresentation_sampling\": null}, \"read_mod_options\": {\"polyg_tail_trimming\": {\"trimming_select\": \"\", \"__current_case__\": 1, \"poly_g_min_len\": null}, \"polyx_tail_trimming\": {\"polyx_trimming_select\": \"\", \"__current_case__\": 1}, \"umi_processing\": {\"umi\": false, \"umi_loc\": \"\", \"umi_len\": null, \"umi_prefix\": \"\"}, \"cutting_by_quality_options\": {\"cut_by_quality5\": false, \"cut_by_quality3\": false, \"cut_window_size\": null, \"cut_mean_quality\": null}, \"base_correction_options\": {\"correction\": false}}, \"single_paired\": {\"single_paired_selector\": \"paired_collection\", \"__current_case__\": 2, \"paired_input\": {\"__class__\": \"ConnectedValue\"}, \"adapter_trimming_options\": {\"disable_adapter_trimming\": false, \"adapter_sequence1\": \"\", \"adapter_sequence2\": \"\"}, \"global_trimming_options\": {\"trim_front1\": null, \"trim_tail1\": null, \"trim_front2\": null, \"trim_tail2\": null}}, \"__page__\": null, \"__rerun_remap_job_id__\": null}", + "tool_version": "0.23.2+galaxy0", "type": "tool", "uuid": "fb6601de-99e2-4271-813d-0e97027e54c7", + "when": null, "workflow_outputs": [] }, "6": { @@ -262,14 +232,8 @@ } ], "position": { - "bottom": 610.6499786376953, - "height": 113.59999084472656, - "left": -96.68333435058594, - "right": 103.31666564941406, - "top": 497.04998779296875, - "width": 200, - "x": -96.68333435058594, - "y": 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"workflow_outputs": [ { "label": "snpeff_annotated_vcf", @@ -920,14 +831,8 @@ } ], "position": { - "bottom": 1158.550048828125, - "height": 134, - "left": 1303.316650390625, - "right": 1503.316650390625, - "top": 1024.550048828125, - "width": 200, - "x": 1303.316650390625, - "y": 1024.550048828125 + "left": 1670, + "top": 802.0000457763672 }, "post_job_actions": { "RenameDatasetActionoutput": { @@ -940,7 +845,7 @@ }, "tool_id": "toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_sed_tool/1.1.1", "tool_shed_repository": { - "changeset_revision": "fb4ff3c42cd3", + "changeset_revision": "ddf54b12c295", "name": "text_processing", "owner": "bgruening", "tool_shed": "toolshed.g2.bx.psu.edu" @@ -949,6 +854,7 @@ "tool_version": "1.1.1", "type": "tool", "uuid": "877a609d-d2fa-430d-8b78-2b484af52bda", + "when": null, "workflow_outputs": [ { "label": "ivar_consensus_genome", @@ -968,12 +874,7 @@ "output_name": "output" } }, - "inputs": [ - { - "description": "runtime parameter for tool Concatenate datasets", - "name": "inputs" - } - ], + "inputs": [], "label": null, "name": "Concatenate datasets", "outputs": [ @@ -983,14 +884,8 @@ } ], "position": { - "bottom": 1249.6500396728516, - "height": 113.59999084472656, - "left": 1546.316650390625, - "right": 1746.316650390625, - "top": 1136.050048828125, - "width": 200, - "x": 1546.316650390625, - "y": 1136.050048828125 + "left": 1913, + "top": 913.5000457763672 }, "post_job_actions": { "RenameDatasetActionout_file1": { @@ -1003,15 +898,16 @@ }, "tool_id": "toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_cat/0.1.1", "tool_shed_repository": { - "changeset_revision": "f46f0e4f75c4", + "changeset_revision": "d698c222f354", "name": "text_processing", "owner": "bgruening", "tool_shed": "toolshed.g2.bx.psu.edu" }, - "tool_state": "{\"inputs\": {\"__class__\": \"RuntimeValue\"}, \"queries\": [], \"__page__\": null, \"__rerun_remap_job_id__\": null}", + "tool_state": "{\"inputs\": {\"__class__\": \"ConnectedValue\"}, \"queries\": [], \"__page__\": null, \"__rerun_remap_job_id__\": null}", "tool_version": "0.1.1", "type": "tool", "uuid": "87e8ec0a-d8e8-48f3-b943-04c75152fec2", + "when": null, "workflow_outputs": [ { "label": "combined_multifasta", @@ -1022,7 +918,7 @@ }, "19": { "annotation": "", - "content_id": "toolshed.g2.bx.psu.edu/repos/iuc/pangolin/pangolin/3.1.14+galaxy0", + "content_id": "toolshed.g2.bx.psu.edu/repos/iuc/pangolin/pangolin/4.3+galaxy1", "errors": null, "id": 19, "input_connections": { @@ -1031,12 +927,7 @@ "output_name": "out_file1" } }, - "inputs": [ - { - "description": "runtime parameter for tool Pangolin", - "name": "input1" - } - ], + "inputs": [], "label": null, "name": "Pangolin", "outputs": [ @@ -1046,27 +937,22 @@ } ], "position": { - "bottom": 1166.6500396728516, - "height": 113.59999084472656, - "left": 1810.316650390625, - "right": 2010.316650390625, - "top": 1053.050048828125, - "width": 200, - "x": 1810.316650390625, - "y": 1053.050048828125 + "left": 2177, + 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\"pangolin_data\": {\"source\": \"default\", \"__current_case__\": 0}}, \"expanded_lineage\": false, \"include_header\": true, \"input1\": {\"__class__\": \"ConnectedValue\"}, \"max_ambig\": \"0.5\", \"min_length\": \"10000\", \"usher\": \"false\", \"__page__\": null, \"__rerun_remap_job_id__\": null}", + "tool_version": "4.3+galaxy1", "type": "tool", "uuid": "15ff9812-4ff9-4ac2-9f95-c65bab4eb070", + "when": null, "workflow_outputs": [ { "label": "all_samples_pangolin", @@ -1077,7 +963,7 @@ }, "20": { "annotation": "", - "content_id": "toolshed.g2.bx.psu.edu/repos/iuc/nextclade/nextclade/1.4.1+galaxy0", + "content_id": "toolshed.g2.bx.psu.edu/repos/iuc/nextclade/nextclade/2.7.0+galaxy0", "errors": null, "id": 20, "input_connections": { @@ -1101,27 +987,22 @@ } ], "position": { - "bottom": 1355.550048828125, - "height": 134, - "left": 1809.316650390625, - "right": 2009.316650390625, - "top": 1221.550048828125, - "width": 200, - "x": 1809.316650390625, - "y": 1221.550048828125 + "left": 2176, + "top": 999.0000457763672 }, "post_job_actions": {}, - "tool_id": "toolshed.g2.bx.psu.edu/repos/iuc/nextclade/nextclade/1.4.1+galaxy0", + "tool_id": "toolshed.g2.bx.psu.edu/repos/iuc/nextclade/nextclade/2.7.0+galaxy0", "tool_shed_repository": { - "changeset_revision": "56b1a13d9680", + "changeset_revision": "128ba8da994f", "name": "nextclade", "owner": "iuc", "tool_shed": "toolshed.g2.bx.psu.edu" }, - "tool_state": "{\"adv\": {\"advanced_options\": \"no\", \"__current_case__\": 1}, \"include_header\": \"true\", \"input_fasta\": {\"__class__\": \"RuntimeValue\"}, \"organism\": \"sars-cov-2\", \"outputs\": [\"report_tsv\"], \"__page__\": null, \"__rerun_remap_job_id__\": null}", - "tool_version": "1.4.1+galaxy0", + "tool_state": "{\"adv\": {\"advanced_options\": \"no\", \"__current_case__\": 1}, \"db\": {\"source\": \"download\", \"__current_case__\": 1}, \"include_header\": true, \"input_fasta\": {\"__class__\": \"RuntimeValue\"}, \"organism\": \"sars-cov-2\", \"outputs\": [\"report_tsv\"], \"__page__\": null, \"__rerun_remap_job_id__\": null}", + "tool_version": "2.7.0+galaxy0", "type": "tool", "uuid": "4b821f22-47f8-4251-bc32-5aa167498ba7", + "when": null, "workflow_outputs": [ { "label": "all_samples_nextclade", @@ -1136,5 +1017,6 @@ "ARTIC", "iwc" ], - "uuid": "d784cd9f-b1a1-4a13-8400-5fc5ffa14dd0" -} + "uuid": "3d86e5fc-cad1-4e21-8ecc-d0e6637899bc", + "version": 1 +} \ No newline at end of file diff --git a/workflows/sars-cov-2-variant-calling/sars-cov-2-pe-illumina-artic-variant-calling/.dockstore.yml b/workflows/sars-cov-2-variant-calling/sars-cov-2-pe-illumina-artic-variant-calling/.dockstore.yml index d83eddc48..6ba503958 100644 --- a/workflows/sars-cov-2-variant-calling/sars-cov-2-pe-illumina-artic-variant-calling/.dockstore.yml +++ b/workflows/sars-cov-2-variant-calling/sars-cov-2-pe-illumina-artic-variant-calling/.dockstore.yml @@ -1,7 +1,11 @@ version: 1.2 workflows: - name: 'COVID-19-PE-ARTIC-ILLUMINA' - primaryDescriptorPath: /pe-artic-variation.ga subclass: Galaxy + publish: true + primaryDescriptorPath: /pe-artic-variation.ga testParameterFiles: - - /pe-artic-variation-test.yml + - /pe-artic-variation-tests.yml + authors: + - name: Wolfgang Maier + orcid: 0000-0002-9464-6640 diff --git a/workflows/sars-cov-2-variant-calling/sars-cov-2-pe-illumina-artic-variant-calling/CHANGELOG.md b/workflows/sars-cov-2-variant-calling/sars-cov-2-pe-illumina-artic-variant-calling/CHANGELOG.md index b2711c923..f43e4eac3 100644 --- a/workflows/sars-cov-2-variant-calling/sars-cov-2-pe-illumina-artic-variant-calling/CHANGELOG.md +++ b/workflows/sars-cov-2-variant-calling/sars-cov-2-pe-illumina-artic-variant-calling/CHANGELOG.md @@ -1,5 +1,26 @@ # Changelog +## [0.5.2] 2024-03-05 + +### Automatic update +- `toolshed.g2.bx.psu.edu/repos/iuc/fastp/fastp/0.23.2+galaxy0` was updated to `toolshed.g2.bx.psu.edu/repos/iuc/fastp/fastp/0.23.4+galaxy0` +- `toolshed.g2.bx.psu.edu/repos/iuc/samtools_view/samtools_view/1.9+galaxy2` was updated to `toolshed.g2.bx.psu.edu/repos/iuc/samtools_view/samtools_view/1.15.1+galaxy2` +- `toolshed.g2.bx.psu.edu/repos/devteam/samtools_stats/samtools_stats/2.0.2+galaxy2` was updated to `toolshed.g2.bx.psu.edu/repos/devteam/samtools_stats/samtools_stats/2.0.5` +- `toolshed.g2.bx.psu.edu/repos/iuc/lofreq_indelqual/lofreq_indelqual/2.1.5+galaxy0` was updated to `toolshed.g2.bx.psu.edu/repos/iuc/lofreq_indelqual/lofreq_indelqual/2.1.5+galaxy1` +- `toolshed.g2.bx.psu.edu/repos/iuc/ivar_trim/ivar_trim/1.3.1+galaxy2` was updated to `toolshed.g2.bx.psu.edu/repos/iuc/ivar_trim/ivar_trim/1.4.2+galaxy0` +- `toolshed.g2.bx.psu.edu/repos/iuc/lofreq_call/lofreq_call/2.1.5+galaxy1` was updated to `toolshed.g2.bx.psu.edu/repos/iuc/lofreq_call/lofreq_call/2.1.5+galaxy2` +- `toolshed.g2.bx.psu.edu/repos/iuc/qualimap_bamqc/qualimap_bamqc/2.2.2d+galaxy3` was updated to `toolshed.g2.bx.psu.edu/repos/iuc/qualimap_bamqc/qualimap_bamqc/2.2.2c+galaxy1` +- `toolshed.g2.bx.psu.edu/repos/iuc/ivar_removereads/ivar_removereads/1.3.1+galaxy2` was updated to `toolshed.g2.bx.psu.edu/repos/iuc/ivar_removereads/ivar_removereads/1.4.2+galaxy0` +- `toolshed.g2.bx.psu.edu/repos/iuc/multiqc/multiqc/1.11+galaxy0` was updated to `toolshed.g2.bx.psu.edu/repos/iuc/multiqc/multiqc/1.11+galaxy1` +- `toolshed.g2.bx.psu.edu/repos/iuc/bcftools_annotate/bcftools_annotate/1.10` was updated to `toolshed.g2.bx.psu.edu/repos/iuc/bcftools_annotate/bcftools_annotate/1.15.1+galaxy3` +- `toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_replace_in_line/1.1.2` was updated to `toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_replace_in_line/9.3+galaxy0` + +## [0.5.1] 2023-11-20 + +- Fix author in dockstore +- Use zenodo link instead of googleapis for fastq in test +- Update output test + ## [0.5] - 2022-02-08 ### Fixed diff --git a/workflows/sars-cov-2-variant-calling/sars-cov-2-pe-illumina-artic-variant-calling/pe-artic-variation-test.yml b/workflows/sars-cov-2-variant-calling/sars-cov-2-pe-illumina-artic-variant-calling/pe-artic-variation-tests.yml similarity index 52% rename from workflows/sars-cov-2-variant-calling/sars-cov-2-pe-illumina-artic-variant-calling/pe-artic-variation-test.yml rename to workflows/sars-cov-2-variant-calling/sars-cov-2-pe-illumina-artic-variant-calling/pe-artic-variation-tests.yml index 132610cbf..2b09bc296 100644 --- a/workflows/sars-cov-2-variant-calling/sars-cov-2-pe-illumina-artic-variant-calling/pe-artic-variation-test.yml +++ b/workflows/sars-cov-2-variant-calling/sars-cov-2-pe-illumina-artic-variant-calling/pe-artic-variation-tests.yml @@ -22,17 +22,21 @@ elements: - identifier: forward class: File - location: "https://storage.googleapis.com/nih-sequence-read-archive/sra-src/SRR11578257/VIC818_R1.fq.gz" - filetype: fastqsanger.gz + location: "https://zenodo.org/records/10174466/files/SRR11578257_R1.fastq.gz?download=1" - identifier: reverse class: File - location: "https://storage.googleapis.com/nih-sequence-read-archive/sra-src/SRR11578257/VIC818_R2.fq.gz" - filetype: fastqsanger.gz + location: "https://zenodo.org/records/10174466/files/SRR11578257_R2.fastq.gz?download=1" outputs: annotated_softfiltered_variants: attributes: {} element_tests: SRR11578257: - path: test-data/final_snpeff_annotated_variants.vcf - compare: diff - lines_diff: 10 + asserts: + has_line: + line: "##fileformat=VCFv4.0" + has_line: + line: "#CHROM POS ID REF ALT QUAL FILTER INFO" + has_text_matching: + expression: "NC_045512.2\t174\t.\tG\tC\t[0-9]*\tPASS" + has_text_matching: + expression: "NC_045512.2\t20209\t.\tAGTAGAAATT\tA\t[0-9]*\tPASS\tDP=[0-9]*;AF=0.9[0-9]*;SB=24;DP4=[0-9]*,[0-9]*,[0-9]*,[0-9]*;INDEL;HRUN=1;EFF=CODON_CHANGE_PLUS_CODON_DELETION\\(MODERATE||agtagaaattta/ata|SRNL6649I|7096|ORF1ab|protein_coding|CODING|GU280_gp01|2|A\\),CODON_CHANGE_PLUS_CODON_DELETION\\(MODERATE||agtagaaattta/ata|SRNL197I|345|ORF1ab|protein_coding|CODING|YP_009725310.1|1|A|WARNING_TRANSCRIPT_NO_START_CODON\\)" diff --git a/workflows/sars-cov-2-variant-calling/sars-cov-2-pe-illumina-artic-variant-calling/pe-artic-variation.ga b/workflows/sars-cov-2-variant-calling/sars-cov-2-pe-illumina-artic-variant-calling/pe-artic-variation.ga index 83e0c7df7..3f1530d61 100644 --- a/workflows/sars-cov-2-variant-calling/sars-cov-2-pe-illumina-artic-variant-calling/pe-artic-variation.ga +++ b/workflows/sars-cov-2-variant-calling/sars-cov-2-pe-illumina-artic-variant-calling/pe-artic-variation.ga @@ -11,7 +11,7 @@ "format-version": "0.1", "license": "MIT", "name": "COVID-19: variation analysis on ARTIC PE data", - "release": "0.5", + "release": "0.5.2", "steps": { "0": { "annotation": "Illumina reads from ARTIC assay with fastqsanger encoding", @@ -29,20 +29,15 @@ "name": "Input dataset collection", "outputs": [], "position": { - "bottom": 606.734375, - "height": 102.5625, - "left": -334.703125, - "right": -134.703125, - "top": 504.171875, - "width": 200, - "x": -334.703125, - "y": 504.171875 + "left": 0.0, + "top": 506.796875 }, "tool_id": null, - "tool_state": "{\"optional\": false, \"format\": [\"fastqsanger\", \"fastqsanger.gz\"], \"collection_type\": \"list:paired\"}", + "tool_state": "{\"optional\": false, \"format\": [\"fastqsanger\", \"fastqsanger.gz\"], \"tag\": null, \"collection_type\": \"list:paired\"}", "tool_version": null, "type": "data_collection_input", "uuid": "dfab5b21-4abb-4269-978d-7c7e24479e4a", + "when": null, "workflow_outputs": [] }, "1": { @@ -61,20 +56,15 @@ "name": "Input dataset", "outputs": [], "position": { - "bottom": 983.5, - "height": 102.5625, - 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"cd6caddf-88ee-47a2-b91d-141fd90b046f", + "when": null, "workflow_outputs": [] }, "3": { @@ -125,84 +110,69 @@ "name": "Input dataset", "outputs": [], "position": { - "bottom": 531.5, - "height": 102.5625, - "left": 626.921875, - "right": 826.921875, - "top": 428.9375, - "width": 200, - "x": 626.921875, - "y": 428.9375 + "left": 961.625, + "top": 431.5625 }, "tool_id": null, - "tool_state": "{\"optional\": false, \"format\": [\"tabular\"]}", + "tool_state": "{\"optional\": false, \"format\": [\"tabular\"], \"tag\": null}", "tool_version": null, "type": "data_input", "uuid": "686cdb4d-a3da-468f-af92-8b04e1824ae0", + "when": null, "workflow_outputs": [] }, "4": { - "annotation": "Maximum allele-frequency allowed for a primer binding site mutation to trigger amplicon removal. Variants with AF values above this threshold are treated as fixed variants, which won't generate amplicon bias.", + "annotation": "Minimum allele-frequency required for a candidate primer binding site mutation to trigger amplicon removal. Variants with AF values below this threshold are treated as possible false-positives, which are not worth the coverage loss associated with amplicon removal.", "content_id": null, "errors": null, "id": 4, "input_connections": {}, "inputs": [ { - "description": "Maximum allele-frequency allowed for a primer binding site mutation to trigger amplicon removal. Variants with AF values above this threshold are treated as fixed variants, which won't generate amplicon bias.", - "name": "Read removal maximum AF" + "description": "Minimum allele-frequency required for a candidate primer binding site mutation to trigger amplicon removal. 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"inputs": [], + "inputs": [ + { + "description": "runtime parameter for tool Flatten collection", + "name": "input" + } + ], "label": null, - "name": "Flatten Collection", + "name": "Flatten collection", "outputs": [ { "name": "output", @@ -1083,14 +1130,8 @@ } ], "position": { - "bottom": 920.828125, - "height": 133.953125, - "left": 1620.953125, - "right": 1820.953125, - "top": 786.875, - "width": 200, - "x": 1620.953125, - "y": 786.875 + "left": 1955.65625, + "top": 789.5 }, "post_job_actions": {}, "tool_id": "__FLATTEN__", @@ -1098,6 +1139,7 @@ "tool_version": "1.0.0", "type": "tool", "uuid": "396c9b85-8ec7-405a-a742-5b47e40cf0de", + "when": null, "workflow_outputs": [ { "label": "bamqc_raw_output_flattened", @@ -1106,84 +1148,80 @@ } ] }, - "23": { + "24": { "annotation": "", - "content_id": "toolshed.g2.bx.psu.edu/repos/iuc/ivar_removereads/ivar_removereads/1.3.1+galaxy2", + "content_id": "toolshed.g2.bx.psu.edu/repos/iuc/lofreq_call/lofreq_call/2.1.5+galaxy2", "errors": null, - 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"tool_version": "1.10", + "tool_state": "{\"input_file\": {\"__class__\": \"ConnectedValue\"}, \"output_type\": \"v\", \"sec_annofile\": {\"columns\": \"QUAL,INFO\", \"annofile\": {\"anno_fmt\": \"vcf\", \"__current_case__\": 1, \"annotations\": {\"__class__\": \"ConnectedValue\"}}, \"mark_sites\": \"-AmpliconBias\", \"min_overlap\": \"\", \"set_id\": \"\"}, \"sec_annotate\": {\"remove\": \"\", \"rename_chrs\": {\"__class__\": \"RuntimeValue\"}, \"rename_annots\": {\"__class__\": \"RuntimeValue\"}}, \"sec_restrict\": {\"include\": \"\", \"exclude\": \"\", \"collapse\": null, \"regions\": {\"regions_src\": \"__none__\", \"__current_case__\": 0}, \"regions_overlap\": null, \"samples\": \"\", \"invert_samples\": false, \"samples_file\": {\"__class__\": \"RuntimeValue\"}, \"invert_samples_file\": false}, \"__page__\": null, \"__rerun_remap_job_id__\": null}", + "tool_version": "1.15.1+galaxy3", "type": "tool", "uuid": "c466b7da-f50c-4093-9eb5-c014ba41c1b8", + "when": null, "workflow_outputs": [ { "label": "variants_fixed_partial", @@ -1367,15 +1358,24 @@ "id": 27, "input_connections": { "filter_expression|expr": { - "id": 18, + "id": 10, "output_name": "out1" }, "input": { - "id": 25, + "id": 24, "output_name": "variants" } }, - "inputs": [], + "inputs": [ + { + "description": "runtime parameter for tool SnpSift Filter", + "name": "filter_expression" + }, + { + "description": "runtime parameter for tool SnpSift Filter", + "name": "input" + } + ], "label": null, "name": "SnpSift Filter", "outputs": [ @@ -1385,14 +1385,8 @@ } ], "position": { - "bottom": 510.765625, - "height": 164.34375, - "left": 2321, - "right": 2521, - "top": 346.421875, - "width": 200, - "x": 2321, - "y": 346.421875 + "left": 2655.703125, + "top": 349.046875 }, "post_job_actions": {}, "tool_id": "toolshed.g2.bx.psu.edu/repos/iuc/snpsift/snpSift_filter/4.3+t.galaxy1", @@ -1402,10 +1396,11 @@ "owner": "iuc", "tool_shed": "toolshed.g2.bx.psu.edu" }, - "tool_state": "{\"filter_expression\": {\"type\": \"simple\", \"__current_case__\": 0, \"expr\": {\"__class__\": \"ConnectedValue\"}}, \"filtering\": {\"mode\": \"entries\", \"__current_case__\": 0}, \"input\": {\"__class__\": \"ConnectedValue\"}, \"inverse\": \"false\", \"__page__\": null, \"__rerun_remap_job_id__\": null}", + "tool_state": "{\"filter_expression\": {\"type\": \"simple\", \"__current_case__\": 0, \"expr\": {\"__class__\": \"ConnectedValue\"}}, \"filtering\": {\"mode\": \"entries\", \"__current_case__\": 0}, \"input\": {\"__class__\": \"ConnectedValue\"}, \"inverse\": false, \"__page__\": null, \"__rerun_remap_job_id__\": null}", "tool_version": "4.3+t.galaxy1", "type": "tool", "uuid": "c3d5ad01-385e-4405-bdc1-51f01dee4071", + "when": null, "workflow_outputs": [ { "label": "preliminary_variants_2_filtered", @@ -1433,7 +1428,20 @@ "output_name": "output" } }, - "inputs": [], + "inputs": [ + { + "description": "runtime parameter for tool VCF-VCFintersect:", + "name": "reference_source" + }, + { + "description": "runtime parameter for tool VCF-VCFintersect:", + "name": "vcf_input1" + }, + { + "description": "runtime parameter for tool VCF-VCFintersect:", + "name": "vcf_input2" + } + ], "label": null, "name": "VCF-VCFintersect:", "outputs": [ @@ -1443,14 +1451,8 @@ } ], "position": { - "bottom": 693.109375, - "height": 194.734375, - "left": 2551.15625, - "right": 2751.15625, - "top": 498.375, - "width": 200, - "x": 2551.15625, - "y": 498.375 + "left": 2885.859375, + "top": 501.0 }, "post_job_actions": {}, "tool_id": "toolshed.g2.bx.psu.edu/repos/devteam/vcfvcfintersect/vcfvcfintersect/1.0.0_rc3+galaxy0", @@ -1460,10 +1462,11 @@ "owner": "devteam", "tool_shed": "toolshed.g2.bx.psu.edu" }, - "tool_state": "{\"adv_options\": {\"adv_options_selector\": \"no\", \"__current_case__\": 0}, \"invert\": \"true\", \"isect_union\": \"-i\", \"loci\": \"false\", \"reference_source\": {\"reference_source_selector\": \"history\", \"__current_case__\": 1, \"ref_file\": {\"__class__\": \"ConnectedValue\"}}, \"vcf_input1\": {\"__class__\": \"ConnectedValue\"}, \"vcf_input2\": {\"__class__\": \"ConnectedValue\"}, \"window_size\": \"0\", \"__page__\": null, \"__rerun_remap_job_id__\": null}", + "tool_state": "{\"adv_options\": {\"adv_options_selector\": \"no\", \"__current_case__\": 0}, \"invert\": true, \"isect_union\": \"-i\", \"loci\": false, \"reference_source\": {\"reference_source_selector\": \"history\", \"__current_case__\": 1, \"ref_file\": {\"__class__\": \"ConnectedValue\"}}, \"vcf_input1\": {\"__class__\": \"ConnectedValue\"}, \"vcf_input2\": {\"__class__\": \"ConnectedValue\"}, \"window_size\": \"0\", \"__page__\": null, \"__rerun_remap_job_id__\": null}", "tool_version": "1.0.0_rc3+galaxy0", "type": "tool", "uuid": "816eeae7-4ad5-4f2d-b709-285931d2be4b", + "when": null, "workflow_outputs": [ { "label": "lost_filter_passing_variants", @@ -1474,7 +1477,7 @@ }, "29": { "annotation": "", - "content_id": "toolshed.g2.bx.psu.edu/repos/iuc/bcftools_annotate/bcftools_annotate/1.10", + "content_id": "toolshed.g2.bx.psu.edu/repos/iuc/bcftools_annotate/bcftools_annotate/1.15.1+galaxy3", "errors": null, "id": 29, "input_connections": { @@ -1487,7 +1490,24 @@ "output_name": "out_file1" } }, - "inputs": [], + "inputs": [ + { + "description": "runtime parameter for tool bcftools annotate", + "name": "input_file" + }, + { + "description": "runtime parameter for tool bcftools annotate", + "name": "sec_annotate" + }, + { + "description": "runtime parameter for tool bcftools annotate", + "name": "sec_annotate" + }, + { + "description": "runtime parameter for tool bcftools annotate", + "name": "sec_restrict" + } + ], "label": null, "name": "bcftools annotate", "outputs": [ @@ -1497,27 +1517,22 @@ } ], "position": { - "bottom": 878.78125, - "height": 225.125, - "left": 2778.25, - "right": 2978.25, - "top": 653.65625, - "width": 200, - "x": 2778.25, - "y": 653.65625 + "left": 3112.953125, + "top": 656.28125 }, "post_job_actions": {}, - "tool_id": "toolshed.g2.bx.psu.edu/repos/iuc/bcftools_annotate/bcftools_annotate/1.10", + "tool_id": "toolshed.g2.bx.psu.edu/repos/iuc/bcftools_annotate/bcftools_annotate/1.15.1+galaxy3", "tool_shed_repository": { - "changeset_revision": "2436e3bef247", + "changeset_revision": "3f62d4939d54", "name": "bcftools_annotate", "owner": "iuc", "tool_shed": "toolshed.g2.bx.psu.edu" }, - "tool_state": "{\"input_file\": {\"__class__\": \"ConnectedValue\"}, \"output_type\": \"v\", \"sec_annofile\": {\"columns\": \"QUAL,INFO\", \"annofile\": {\"anno_fmt\": \"vcf\", \"__current_case__\": 1, \"annotations\": {\"__class__\": \"ConnectedValue\"}}, \"mark_sites\": \"+AmpliconBias\", \"set_id\": \"\"}, \"sec_annotate\": {\"remove\": \"\", \"rename_chrs\": {\"__class__\": \"RuntimeValue\"}}, \"sec_restrict\": {\"include\": \"\", \"exclude\": \"\", \"collapse\": null, \"regions\": {\"regions_src\": \"__none__\", \"__current_case__\": 0}, \"samples\": \"\", \"invert_samples\": \"false\", \"samples_file\": {\"__class__\": \"RuntimeValue\"}, \"invert_samples_file\": \"false\"}, \"__page__\": null, \"__rerun_remap_job_id__\": null}", - "tool_version": "1.10", + "tool_state": "{\"input_file\": {\"__class__\": \"ConnectedValue\"}, \"output_type\": \"v\", \"sec_annofile\": {\"columns\": \"QUAL,INFO\", \"annofile\": {\"anno_fmt\": \"vcf\", \"__current_case__\": 1, \"annotations\": {\"__class__\": \"ConnectedValue\"}}, \"mark_sites\": \"+AmpliconBias\", \"min_overlap\": \"\", \"set_id\": \"\"}, \"sec_annotate\": {\"remove\": \"\", \"rename_chrs\": {\"__class__\": \"RuntimeValue\"}, \"rename_annots\": {\"__class__\": \"RuntimeValue\"}}, \"sec_restrict\": {\"include\": \"\", \"exclude\": \"\", \"collapse\": null, \"regions\": {\"regions_src\": \"__none__\", \"__current_case__\": 0}, \"regions_overlap\": null, \"samples\": \"\", \"invert_samples\": false, \"samples_file\": {\"__class__\": \"RuntimeValue\"}, \"invert_samples_file\": false}, \"__page__\": null, \"__rerun_remap_job_id__\": null}", + "tool_version": "1.15.1+galaxy3", "type": "tool", "uuid": "c466b7da-f50c-4093-9eb5-c014ba41c1b7", + "when": null, "workflow_outputs": [ { "label": "variants_fixed", @@ -1528,7 +1543,7 @@ }, "30": { "annotation": "", - "content_id": "toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_replace_in_line/1.1.2", + "content_id": "toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_replace_in_line/9.3+galaxy0", "errors": null, "id": 30, "input_connections": { @@ -1537,7 +1552,12 @@ "output_name": "output_file" } }, - "inputs": [], + "inputs": [ + { + "description": "runtime parameter for tool Replace Text", + "name": "infile" + } + ], "label": null, "name": "Replace Text", "outputs": [ @@ -1547,27 +1567,22 @@ } ], "position": { - "bottom": 856.484375, - "height": 113.5625, - "left": 3003.578125, - "right": 3203.578125, - "top": 742.921875, - "width": 200, - "x": 3003.578125, - "y": 742.921875 + "left": 3338.28125, + "top": 745.546875 }, "post_job_actions": {}, - "tool_id": "toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_replace_in_line/1.1.2", + "tool_id": "toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_replace_in_line/9.3+galaxy0", "tool_shed_repository": { - "changeset_revision": "ddf54b12c295", + "changeset_revision": "12615d397df7", "name": "text_processing", "owner": "bgruening", "tool_shed": "toolshed.g2.bx.psu.edu" }, "tool_state": "{\"infile\": {\"__class__\": \"ConnectedValue\"}, \"replacements\": [{\"__index__\": 0, \"find_pattern\": \"^##INFO=\"}, {\"__index__\": 1, \"find_pattern\": \"^##INFO= --min-bq among all bases at the site\\\">\"}], \"__page__\": null, \"__rerun_remap_job_id__\": null}", - "tool_version": "1.1.2", + "tool_version": "9.3+galaxy0", "type": "tool", "uuid": "490dbabb-04ee-426d-a8e5-e2ec9e59de3a", + "when": null, "workflow_outputs": [ { "label": "variants_fixed_header", @@ -1587,7 +1602,20 @@ "output_name": "outfile" } }, - "inputs": [], + "inputs": [ + { + "description": "runtime parameter for tool SnpEff eff:", + "name": "input" + }, + { + "description": "runtime parameter for tool SnpEff eff:", + "name": "intervals" + }, + { + "description": "runtime parameter for tool SnpEff eff:", + "name": "transcripts" + } + ], "label": "SnpEff eff covid19 version", "name": "SnpEff eff:", "outputs": [ @@ -1601,14 +1629,8 @@ } ], "position": { - "bottom": 903.03125, - "height": 306.6875, - "left": 3231.546875, - "right": 3431.546875, - "top": 596.34375, - "width": 200, - "x": 3231.546875, - "y": 596.34375 + "left": 3566.25, + "top": 598.96875 }, "post_job_actions": { "RenameDatasetActionsnpeff_output": { @@ -1626,10 +1648,11 @@ "owner": "iuc", "tool_shed": "toolshed.g2.bx.psu.edu" }, - "tool_state": "{\"annotations\": [\"-formatEff\", \"-classic\"], \"chr\": \"\", \"csvStats\": \"false\", \"filter\": {\"specificEffects\": \"no\", \"__current_case__\": 0}, \"filterOut\": [\"-no-downstream\", \"-no-intergenic\", \"-no-upstream\"], \"generate_stats\": \"true\", \"genome_version\": \"NC_045512.2\", \"input\": {\"__class__\": \"ConnectedValue\"}, \"inputFormat\": \"vcf\", \"intervals\": {\"__class__\": \"RuntimeValue\"}, \"noLog\": \"true\", \"offset\": \"default\", \"outputConditional\": {\"outputFormat\": \"vcf\", \"__current_case__\": 0}, \"transcripts\": {\"__class__\": \"RuntimeValue\"}, \"udLength\": \"0\", \"__page__\": null, \"__rerun_remap_job_id__\": null}", + "tool_state": "{\"annotations\": [\"-formatEff\", \"-classic\"], \"chr\": \"\", \"csvStats\": false, \"filter\": {\"specificEffects\": \"no\", \"__current_case__\": 0}, \"filterOut\": [\"-no-downstream\", \"-no-intergenic\", \"-no-upstream\"], \"generate_stats\": true, \"genome_version\": \"NC_045512.2\", \"input\": {\"__class__\": \"ConnectedValue\"}, \"inputFormat\": \"vcf\", \"intervals\": {\"__class__\": \"RuntimeValue\"}, \"noLog\": true, \"offset\": \"default\", \"outputConditional\": {\"outputFormat\": \"vcf\", \"__current_case__\": 0}, \"transcripts\": {\"__class__\": \"RuntimeValue\"}, \"udLength\": \"0\", \"__page__\": null, \"__rerun_remap_job_id__\": null}", "tool_version": "4.5covid19", "type": "tool", "uuid": "a6b0b8e1-a914-4f59-95d4-87fc47ed4171", + "when": null, "workflow_outputs": [ { "label": "annotated_variants", @@ -1654,7 +1677,12 @@ "output_name": "snpeff_output" } }, - "inputs": [], + "inputs": [ + { + "description": "runtime parameter for tool Lofreq filter", + "name": "invcf" + } + ], "label": null, "name": "Lofreq filter", "outputs": [ @@ -1664,14 +1692,8 @@ } ], "position": { - "bottom": 725.5625, - "height": 113.5625, - "left": 3467.796875, - "right": 3667.796875, - "top": 612, - "width": 200, - "x": 3467.796875, - "y": 612 + "left": 3802.5, + "top": 614.625 }, "post_job_actions": { "RenameDatasetActionoutvcf": { @@ -1689,10 +1711,11 @@ "owner": "iuc", "tool_shed": "toolshed.g2.bx.psu.edu" }, - "tool_state": "{\"af\": {\"af_min\": \"0.0\", \"af_max\": \"0.0\"}, \"coverage\": {\"cov_min\": \"0\", \"cov_max\": \"0\"}, \"filter_by_type\": {\"keep_only\": \"\", \"__current_case__\": 0, \"qual\": {\"snvqual_filter\": {\"snvqual\": \"no\", \"__current_case__\": 0}, \"indelqual_filter\": {\"indelqual\": \"no\", \"__current_case__\": 0}}}, \"flag_or_drop\": \"--print-all\", \"invcf\": {\"__class__\": \"ConnectedValue\"}, \"sb\": {\"sb_filter\": {\"strand_bias\": \"mtc\", \"__current_case__\": 2, \"sb_alpha\": \"0.001\", \"sb_mtc\": \"fdr\", \"sb_compound\": \"true\", \"sb_indels\": \"false\"}}, \"__page__\": null, \"__rerun_remap_job_id__\": null}", + "tool_state": "{\"af\": {\"af_min\": \"0.0\", \"af_max\": \"0.0\"}, \"coverage\": {\"cov_min\": \"0\", \"cov_max\": \"0\"}, \"filter_by_type\": {\"keep_only\": \"\", \"__current_case__\": 0, \"qual\": {\"snvqual_filter\": {\"snvqual\": \"no\", \"__current_case__\": 0}, \"indelqual_filter\": {\"indelqual\": \"no\", \"__current_case__\": 0}}}, \"flag_or_drop\": \"--print-all\", \"invcf\": {\"__class__\": \"ConnectedValue\"}, \"sb\": {\"sb_filter\": {\"strand_bias\": \"mtc\", \"__current_case__\": 2, \"sb_alpha\": \"0.001\", \"sb_mtc\": \"fdr\", \"sb_compound\": true, \"sb_indels\": false}}, \"__page__\": null, \"__rerun_remap_job_id__\": null}", "tool_version": "2.1.5+galaxy0", "type": "tool", "uuid": "4c9e8621-e930-4593-b0be-d84304f86ad4", + "when": null, "workflow_outputs": [ { "label": "annotated_softfiltered_variants", @@ -1708,4 +1731,4 @@ "covid19.galaxyproject.org" ], "uuid": "864427b0-d25a-499c-b437-f83f2d6b83f2" -} +} \ No newline at end of file diff --git a/workflows/sars-cov-2-variant-calling/sars-cov-2-pe-illumina-artic-variant-calling/test-data/final_snpeff_annotated_variants.vcf b/workflows/sars-cov-2-variant-calling/sars-cov-2-pe-illumina-artic-variant-calling/test-data/final_snpeff_annotated_variants.vcf deleted file mode 100644 index 7b0396aa0..000000000 --- a/workflows/sars-cov-2-variant-calling/sars-cov-2-pe-illumina-artic-variant-calling/test-data/final_snpeff_annotated_variants.vcf +++ /dev/null @@ -1,146 +0,0 @@ -##fileformat=VCFv4.0 -##FILTER= -##fileDate=20220208 -##source=lofreq call --verbose --ref reference.fa --call-indels --min-cov 5 --max-depth 1000000 --min-bq 30 --min-alt-bq 30 --min-mq 20 --max-mq 255 --min-jq 0 --min-alt-jq 0 --def-alt-jq 0 --sig 0.0005 --bonf dynamic --no-default-filter --no-default-filter -r NC_045512.2:1-7475 -o /data/jwd/main/040/985/40985665/tmp/lofreq2_call_parallelfwy160pl/0.vcf.gz reads.bam -##reference=reference.fa -##INFO= -##INFO= --min-bq among all bases at the site"> -##INFO= -##INFO= -##INFO= -##INFO= -##INFO= -##FILTER= -##FILTER= -##FILTER= -##FILTER= -##contig= -##INFO= -##bcftools_annotateVersion=1.10.2+htslib-1.10.2 -##bcftools_annotateCommand=annotate --columns QUAL,INFO --annotations annotations.vcf.gz --mark-sites -AmpliconBias --output-type v --threads 1 input.vcf.gz; Date=Tue Feb 8 11:30:28 2022 -##bcftools_annotateCommand=annotate --columns QUAL,INFO --annotations annotations.vcf.gz --mark-sites +AmpliconBias --output-type v --threads 1 input.vcf.gz; Date=Tue Feb 8 11:33:07 2022 -##SnpEffVersion="4.5covid19 (build 2020-04-15 22:26), by Pablo Cingolani" -##SnpEffCmd="SnpEff -i vcf -o vcf -formatEff -classic -no-downstream -no-intergenic -no-upstream -stats /data/jwd/main/040/985/40985835/outputs/galaxy_dataset_6f12bbaa-aa2a-4071-ab5d-da54e3a4f14a.dat NC_045512.2 /data/dnb05/galaxy_db/files/6/2/f/dataset_62f5f01c-2e71-49f6-9ec8-206545e560b1.dat " -##INFO= -##INFO= -##INFO= -##FILTER= 0.001000"> -#CHROM POS ID REF ALT QUAL FILTER INFO -NC_045512.2 174 . 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TA T 89 PASS DP=19291;AF=0.00057;SB=1;DP4=16545,2735,9,2;INDEL;HRUN=3;EFF=FRAME_SHIFT(HIGH||aaa/|K164|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|T|INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||aaa/|K164|179|ORF1ab|protein_coding|CODING|YP_009725297.1|1|T|WARNING_TRANSCRIPT_NO_STOP_CODON&INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||aaa/|K164|179|ORF1ab|protein_coding|CODING|YP_009742608.1|1|T|WARNING_TRANSCRIPT_NO_STOP_CODON&INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||aaa/|K164|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|T|INFO_REALIGN_3_PRIME);LOF=(ORF1ab|GU280_gp01|28|0.07) -NC_045512.2 802 . 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C A 1172 sb_fdr DP=29782;AF=0.007253;SB=492;DP4=16581,12865,289,18;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aaC/aaA|N188K|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|A),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aaC/aaA|N188K|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|A),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aaC/aaA|N8K|637|ORF1ab|protein_coding|CODING|YP_009725298.1|1|A|WARNING_TRANSCRIPT_NO_START_CODON),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aaC/aaA|N8K|637|ORF1ab|protein_coding|CODING|YP_009742609.1|1|A|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 840 . G C 156 sb_fdr DP=28966;AF=0.002037;SB=204;DP4=15043,13776,9,96;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gGc/gCc|G192A|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|C),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gGc/gCc|G192A|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|C),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gGc/gCc|G12A|637|ORF1ab|protein_coding|CODING|YP_009725298.1|1|C|WARNING_TRANSCRIPT_NO_START_CODON),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gGc/gCc|G12A|637|ORF1ab|protein_coding|CODING|YP_009742609.1|1|C|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 844 . T C 146 sb_fdr DP=29542;AF=0.001625;SB=155;DP4=14184,15238,86,12;EFF=SYNONYMOUS_CODING(LOW|SILENT|ccT/ccC|P193|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|C),SYNONYMOUS_CODING(LOW|SILENT|ccT/ccC|P193|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|C),SYNONYMOUS_CODING(LOW|SILENT|ccT/ccC|P13|637|ORF1ab|protein_coding|CODING|YP_009725298.1|1|C|WARNING_TRANSCRIPT_NO_START_CODON),SYNONYMOUS_CODING(LOW|SILENT|ccT/ccC|P13|637|ORF1ab|protein_coding|CODING|YP_009742609.1|1|C|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 856 . T C 355 sb_fdr DP=32698;AF=0.003395;SB=484;DP4=13586,18928,158,8;EFF=SYNONYMOUS_CODING(LOW|SILENT|ccT/ccC|P197|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|C),SYNONYMOUS_CODING(LOW|SILENT|ccT/ccC|P197|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|C),SYNONYMOUS_CODING(LOW|SILENT|ccT/ccC|P17|637|ORF1ab|protein_coding|CODING|YP_009725298.1|1|C|WARNING_TRANSCRIPT_NO_START_CODON),SYNONYMOUS_CODING(LOW|SILENT|ccT/ccC|P17|637|ORF1ab|protein_coding|CODING|YP_009742609.1|1|C|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 857 . C T 218 PASS DP=32971;AF=0.008765;SB=0;DP4=13353,19162,165,242;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Ctt/Ttt|L198F|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Ctt/Ttt|L198F|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Ctt/Ttt|L18F|637|ORF1ab|protein_coding|CODING|YP_009725298.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Ctt/Ttt|L18F|637|ORF1ab|protein_coding|CODING|YP_009742609.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 910 . G T 174 sb_fdr DP=28868;AF=0.00194;SB=542;DP4=6328,22426,94,5;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|ttG/ttT|L215F|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|ttG/ttT|L215F|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|ttG/ttT|L35F|637|ORF1ab|protein_coding|CODING|YP_009725298.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|ttG/ttT|L35F|637|ORF1ab|protein_coding|CODING|YP_009742609.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 917 . C A 108 sb_fdr DP=27348;AF=0.001536;SB=490;DP4=4994,22252,73,3;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Caa/Aaa|Q218K|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|A),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Caa/Aaa|Q218K|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|A),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Caa/Aaa|Q38K|637|ORF1ab|protein_coding|CODING|YP_009725298.1|1|A|WARNING_TRANSCRIPT_NO_START_CODON),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Caa/Aaa|Q38K|637|ORF1ab|protein_coding|CODING|YP_009742609.1|1|A|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 1031 . G A 215 sb_fdr DP=30671;AF=0.001826;SB=1113;DP4=27149,3356,0,117;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gaa/Aaa|E256K|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|A),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gaa/Aaa|E256K|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|A),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gaa/Aaa|E76K|637|ORF1ab|protein_coding|CODING|YP_009725298.1|1|A|WARNING_TRANSCRIPT_NO_START_CODON),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gaa/Aaa|E76K|637|ORF1ab|protein_coding|CODING|YP_009742609.1|1|A|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 1048 . G A 136 sb_fdr DP=32606;AF=0.001411;SB=823;DP4=27571,4885,1,103;EFF=SYNONYMOUS_CODING(LOW|SILENT|aaG/aaA|K261|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|A),SYNONYMOUS_CODING(LOW|SILENT|aaG/aaA|K261|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|A),SYNONYMOUS_CODING(LOW|SILENT|aaG/aaA|K81|637|ORF1ab|protein_coding|CODING|YP_009725298.1|1|A|WARNING_TRANSCRIPT_NO_START_CODON),SYNONYMOUS_CODING(LOW|SILENT|aaG/aaA|K81|637|ORF1ab|protein_coding|CODING|YP_009742609.1|1|A|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 1049 . A G 164 PASS DP=32789;AF=0.001464;SB=0;DP4=27628,5098,46,7;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Aaa/Gaa|K262E|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|G),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Aaa/Gaa|K262E|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|G),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Aaa/Gaa|K82E|637|ORF1ab|protein_coding|CODING|YP_009725298.1|1|G|WARNING_TRANSCRIPT_NO_START_CODON),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Aaa/Gaa|K82E|637|ORF1ab|protein_coding|CODING|YP_009742609.1|1|G|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 1080 . A T 479 sb_fdr DP=38236;AF=0.002981;SB=1031;DP4=30151,7877,17,185;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aAt/aTt|N272I|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aAt/aTt|N272I|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aAt/aTt|N92I|637|ORF1ab|protein_coding|CODING|YP_009725298.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aAt/aTt|N92I|637|ORF1ab|protein_coding|CODING|YP_009742609.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 1090 . T C 1272 sb_fdr DP=42746;AF=0.005498;SB=1731;DP4=30123,12234,7,348;EFF=SYNONYMOUS_CODING(LOW|SILENT|ttT/ttC|F275|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|C),SYNONYMOUS_CODING(LOW|SILENT|ttT/ttC|F275|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|C),SYNONYMOUS_CODING(LOW|SILENT|ttT/ttC|F95|637|ORF1ab|protein_coding|CODING|YP_009725298.1|1|C|WARNING_TRANSCRIPT_NO_START_CODON),SYNONYMOUS_CODING(LOW|SILENT|ttT/ttC|F95|637|ORF1ab|protein_coding|CODING|YP_009742609.1|1|C|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 1101 . C T 545 sb_fdr DP=44261;AF=0.004835;SB=491;DP4=29108,14738,324,5;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|tCc/tTc|S279F|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|tCc/tTc|S279F|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|tCc/tTc|S99F|637|ORF1ab|protein_coding|CODING|YP_009725298.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|tCc/tTc|S99F|637|ORF1ab|protein_coding|CODING|YP_009742609.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 1103 . AT A 236 PASS DP=42808;AF=0.001051;SB=80;DP4=28219,14995,45,0;INDEL;HRUN=1;EFF=FRAME_SHIFT(HIGH||ata/|I280|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|A),FRAME_SHIFT(HIGH||ata/|I280|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|A),FRAME_SHIFT(HIGH||ata/|I100|637|ORF1ab|protein_coding|CODING|YP_009725298.1|1|A|WARNING_TRANSCRIPT_NO_START_CODON),FRAME_SHIFT(HIGH||ata/|I100|637|ORF1ab|protein_coding|CODING|YP_009742609.1|1|A|WARNING_TRANSCRIPT_NO_START_CODON);LOF=(ORF1ab|GU280_gp01|28|0.11) -NC_045512.2 1111 . G A 707 PASS DP=41454;AF=0.003836;SB=362;DP4=24914,16287,34,168;EFF=SYNONYMOUS_CODING(LOW|SILENT|aaG/aaA|K282|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|A),SYNONYMOUS_CODING(LOW|SILENT|aaG/aaA|K282|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|A),SYNONYMOUS_CODING(LOW|SILENT|aaG/aaA|K102|637|ORF1ab|protein_coding|CODING|YP_009725298.1|1|A|WARNING_TRANSCRIPT_NO_START_CODON),SYNONYMOUS_CODING(LOW|SILENT|aaG/aaA|K102|637|ORF1ab|protein_coding|CODING|YP_009742609.1|1|A|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 1135 . G A 2714 sb_fdr DP=38398;AF=0.014818;SB=2362;DP4=18549,18526,992,37;EFF=SYNONYMOUS_CODING(LOW|SILENT|aaG/aaA|K290|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|A),SYNONYMOUS_CODING(LOW|SILENT|aaG/aaA|K290|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|A),SYNONYMOUS_CODING(LOW|SILENT|aaG/aaA|K110|637|ORF1ab|protein_coding|CODING|YP_009725298.1|1|A|WARNING_TRANSCRIPT_NO_START_CODON),SYNONYMOUS_CODING(LOW|SILENT|aaG/aaA|K110|637|ORF1ab|protein_coding|CODING|YP_009742609.1|1|A|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 1142 . C CA 80 PASS DP=34704;AF=0.000461;SB=55;DP4=15837,19246,16,0;INDEL;HRUN=2;EFF=FRAME_SHIFT(HIGH||ctt/cAtt|L293H?|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|CA),FRAME_SHIFT(HIGH||ctt/cAtt|L293H?|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|CA),FRAME_SHIFT(HIGH||ctt/cAtt|L113H?|637|ORF1ab|protein_coding|CODING|YP_009725298.1|1|CA|WARNING_TRANSCRIPT_NO_START_CODON),FRAME_SHIFT(HIGH||ctt/cAtt|L113H?|637|ORF1ab|protein_coding|CODING|YP_009742609.1|1|CA|WARNING_TRANSCRIPT_NO_START_CODON);LOF=(ORF1ab|GU280_gp01|28|0.11) -NC_045512.2 1150 . C G 546 sb_fdr DP=32688;AF=0.003518;SB=550;DP4=12495,19912,164,9;EFF=SYNONYMOUS_CODING(LOW|SILENT|ggC/ggG|G295|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|G),SYNONYMOUS_CODING(LOW|SILENT|ggC/ggG|G295|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|G),SYNONYMOUS_CODING(LOW|SILENT|ggC/ggG|G115|637|ORF1ab|protein_coding|CODING|YP_009725298.1|1|G|WARNING_TRANSCRIPT_NO_START_CODON),SYNONYMOUS_CODING(LOW|SILENT|ggC/ggG|G115|637|ORF1ab|protein_coding|CODING|YP_009742609.1|1|G|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 1161 . G A 128 sb_fdr DP=31984;AF=0.003002;SB=187;DP4=10550,21293,1,118;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aGa/aAa|R299K|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|A),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aGa/aAa|R299K|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|A),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aGa/aAa|R119K|637|ORF1ab|protein_coding|CODING|YP_009725298.1|1|A|WARNING_TRANSCRIPT_NO_START_CODON),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aGa/aAa|R119K|637|ORF1ab|protein_coding|CODING|YP_009742609.1|1|A|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 1163 . A T 172 sb_fdr DP=32405;AF=0.002685;SB=66;DP4=10241,21980,21,134;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Att/Ttt|I300F|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Att/Ttt|I300F|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Att/Ttt|I120F|637|ORF1ab|protein_coding|CODING|YP_009725298.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Att/Ttt|I120F|637|ORF1ab|protein_coding|CODING|YP_009742609.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 1181 . G T 305 sb_fdr DP=35644;AF=0.003254;SB=139;DP4=7010,28459,1,165;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gtt/Ttt|V306F|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gtt/Ttt|V306F|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gtt/Ttt|V126F|637|ORF1ab|protein_coding|CODING|YP_009725298.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gtt/Ttt|V126F|637|ORF1ab|protein_coding|CODING|YP_009742609.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 1209 . T A 84 sb_fdr DP=34413;AF=0.002034;SB=909;DP4=5698,28577,126,4;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aTg/aAg|M315K|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|A),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aTg/aAg|M315K|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|A),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aTg/aAg|M135K|637|ORF1ab|protein_coding|CODING|YP_009725298.1|1|A|WARNING_TRANSCRIPT_NO_START_CODON),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aTg/aAg|M135K|637|ORF1ab|protein_coding|CODING|YP_009742609.1|1|A|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 1254 . C T 311 sb_fdr DP=20807;AF=0.003316;SB=766;DP4=2428,18271,85,1;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|tCa/tTa|S330L|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|tCa/tTa|S330L|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|tCa/tTa|S150L|637|ORF1ab|protein_coding|CODING|YP_009725298.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|tCa/tTa|S150L|637|ORF1ab|protein_coding|CODING|YP_009742609.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 1594 . C T 414 PASS DP=14;AF=0.857143;SB=0;DP4=0,0,4,10;EFF=SYNONYMOUS_CODING(LOW|SILENT|tcC/tcT|S443|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|T),SYNONYMOUS_CODING(LOW|SILENT|tcC/tcT|S443|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|T),SYNONYMOUS_CODING(LOW|SILENT|tcC/tcT|S263|637|ORF1ab|protein_coding|CODING|YP_009725298.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON),SYNONYMOUS_CODING(LOW|SILENT|tcC/tcT|S263|637|ORF1ab|protein_coding|CODING|YP_009742609.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 3037 . C T 3214 PASS DP=113;AF=0.840708;SB=13;DP4=7,4,31,70;EFF=SYNONYMOUS_CODING(LOW|SILENT|ttC/ttT|F924|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|T),SYNONYMOUS_CODING(LOW|SILENT|ttC/ttT|F924|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|T),SYNONYMOUS_CODING(LOW|SILENT|ttC/ttT|F106|1944|ORF1ab|protein_coding|CODING|YP_009725299.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON),SYNONYMOUS_CODING(LOW|SILENT|ttC/ttT|F106|1944|ORF1ab|protein_coding|CODING|YP_009742610.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 3177 . C T 132 PASS DP=69;AF=0.086957;SB=1;DP4=22,41,1,5;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cCt/cTt|P971L|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cCt/cTt|P971L|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cCt/cTt|P153L|1944|ORF1ab|protein_coding|CODING|YP_009725299.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cCt/cTt|P153L|1944|ORF1ab|protein_coding|CODING|YP_009742610.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 3874 . C T 275 PASS DP=18;AF=0.5;SB=0;DP4=9,0,9,0;EFF=SYNONYMOUS_CODING(LOW|SILENT|atC/atT|I1203|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|T),SYNONYMOUS_CODING(LOW|SILENT|atC/atT|I1203|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|T),SYNONYMOUS_CODING(LOW|SILENT|atC/atT|I385|1944|ORF1ab|protein_coding|CODING|YP_009725299.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON),SYNONYMOUS_CODING(LOW|SILENT|atC/atT|I385|1944|ORF1ab|protein_coding|CODING|YP_009742610.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 4218 . GA G 104 PASS DP=102;AF=0.039216;SB=1;DP4=60,38,2,2;INDEL;HRUN=4;EFF=FRAME_SHIFT(HIGH||aaa/|K1319|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|G|INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||aaa/|K1319|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|G|INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||aaa/|K501|1944|ORF1ab|protein_coding|CODING|YP_009725299.1|1|G|WARNING_TRANSCRIPT_NO_START_CODON&INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||aaa/|K501|1944|ORF1ab|protein_coding|CODING|YP_009742610.1|1|G|WARNING_TRANSCRIPT_NO_START_CODON&INFO_REALIGN_3_PRIME);LOF=(ORF1ab|GU280_gp01|28|0.14) -NC_045512.2 6285 . C T 359 PASS DP=16;AF=0.6875;SB=0;DP4=4,1,8,3;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aCc/aTc|T2007I|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aCc/aTc|T2007I|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aCc/aTc|T1189I|1944|ORF1ab|protein_coding|CODING|YP_009725299.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aCc/aTc|T1189I|1944|ORF1ab|protein_coding|CODING|YP_009742610.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 6696 . C CT 124 PASS DP=156;AF=0.032051;SB=0;DP4=43,112,1,4;INDEL;HRUN=4;EFF=FRAME_SHIFT(HIGH||ctt/Tctt|L2146S?|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|CT|INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||ctt/Tctt|L2146S?|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|CT|INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||ctt/Tctt|L1328S?|1944|ORF1ab|protein_coding|CODING|YP_009725299.1|1|CT|WARNING_TRANSCRIPT_NO_START_CODON&INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||ctt/Tctt|L1328S?|1944|ORF1ab|protein_coding|CODING|YP_009742610.1|1|CT|WARNING_TRANSCRIPT_NO_START_CODON&INFO_REALIGN_3_PRIME);LOF=(ORF1ab|GU280_gp01|28|0.14) -NC_045512.2 8290 . C T 3497 PASS DP=125;AF=0.84;SB=9;DP4=9,9,32,75;EFF=SYNONYMOUS_CODING(LOW|SILENT|ctC/ctT|L2675|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|T),SYNONYMOUS_CODING(LOW|SILENT|ctC/ctT|L2675|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|T),SYNONYMOUS_CODING(LOW|SILENT|ctC/ctT|L1857|1944|ORF1ab|protein_coding|CODING|YP_009725299.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON),SYNONYMOUS_CODING(LOW|SILENT|ctC/ctT|L1857|1944|ORF1ab|protein_coding|CODING|YP_009742610.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 8782 . C T 132 PASS DP=18;AF=0.277778;SB=0;DP4=7,5,3,3;EFF=SYNONYMOUS_CODING(LOW|SILENT|agC/agT|S2839|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|T),SYNONYMOUS_CODING(LOW|SILENT|agC/agT|S2839|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|T),SYNONYMOUS_CODING(LOW|SILENT|agC/agT|S76|499|ORF1ab|protein_coding|CODING|YP_009725300.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON),SYNONYMOUS_CODING(LOW|SILENT|agC/agT|S76|499|ORF1ab|protein_coding|CODING|YP_009742611.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 11109 . C T 3912 PASS DP=126;AF=0.896825;SB=0;DP4=9,0,110,7;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gCt/gTt|A3615V|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gCt/gTt|A3615V|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gCt/gTt|A46V|289|ORF1ab|protein_coding|CODING|YP_009725302.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gCt/gTt|A46V|289|ORF1ab|protein_coding|CODING|YP_009742613.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 11653 . CT C 136 PASS DP=244;AF=0.02459;SB=2;DP4=190,48,4,2;INDEL;HRUN=4;EFF=FRAME_SHIFT(HIGH||tgt/|C3798|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|C|INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||tgt/|C3798|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|C|INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||tgt/|C229|289|ORF1ab|protein_coding|CODING|YP_009725302.1|1|C|WARNING_TRANSCRIPT_NO_START_CODON&INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||tgt/|C229|289|ORF1ab|protein_coding|CODING|YP_009742613.1|1|C|WARNING_TRANSCRIPT_NO_START_CODON&INFO_REALIGN_3_PRIME);LOF=(ORF1ab|GU280_gp01|28|0.14) -NC_045512.2 12593 . C T 204 sb_fdr DP=6221;AF=0.00852;SB=102;DP4=4312,1814,78,1;EFF=STOP_GAINED(HIGH|NONSENSE|Caa/Taa|Q4110*|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|T),STOP_GAINED(HIGH|NONSENSE|Caa/Taa|Q4110*|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|T),STOP_GAINED(HIGH|NONSENSE|Caa/Taa|Q168*|197|ORF1ab|protein_coding|CODING|YP_009725304.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON),STOP_GAINED(HIGH|NONSENSE|Caa/Taa|Q168*|197|ORF1ab|protein_coding|CODING|YP_009742615.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON);LOF=(ORF1ab|GU280_gp01|28|0.04);NMD=(ORF1ab|GU280_gp01|28|0.04) -NC_045512.2 12637 . G C 98 sb_fdr DP=6953;AF=0.008773;SB=176;DP4=2486,4359,0,91;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|tgG/tgC|W4124C|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|C),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|tgG/tgC|W4124C|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|C),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|tgG/tgC|W182C|197|ORF1ab|protein_coding|CODING|YP_009725304.1|1|C|WARNING_TRANSCRIPT_NO_START_CODON),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|tgG/tgC|W182C|197|ORF1ab|protein_coding|CODING|YP_009742615.1|1|C|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 12662 . G A 344 PASS DP=7068;AF=0.006084;SB=2;DP4=1489,5513,11,34;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gcc/Acc|A4133T|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|A),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gcc/Acc|A4133T|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|A),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gcc/Acc|A191T|197|ORF1ab|protein_coding|CODING|YP_009725304.1|1|A|WARNING_TRANSCRIPT_NO_START_CODON),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gcc/Acc|A191T|197|ORF1ab|protein_coding|CODING|YP_009742615.1|1|A|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 13228 . T G 139 PASS DP=252;AF=0.055556;SB=27;DP4=81,154,0,16;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|ttT/ttG|F4321L|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|G),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|ttT/ttG|F4321L|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|G),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|ttT/ttG|F68L|138|ORF1ab|protein_coding|CODING|YP_009725306.1|1|G|WARNING_TRANSCRIPT_NO_START_CODON),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|ttT/ttG|F68L|138|ORF1ab|protein_coding|CODING|YP_009742617.1|1|G|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 14398 . G T 122 PASS DP=111;AF=0.054054;SB=1;DP4=55,50,4,2;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gtg/Ttg|V4712L|7096|ORF1ab|protein_coding|CODING|GU280_gp01|2|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gtg/Ttg|V320L|931|ORF1ab|protein_coding|CODING|YP_009725307.1|2|T|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 14408 . C T 3281 PASS DP=113;AF=0.858407;SB=2;DP4=4,7,50,52;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cCt/cTt|P4715L|7096|ORF1ab|protein_coding|CODING|GU280_gp01|2|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cCt/cTt|P323L|931|ORF1ab|protein_coding|CODING|YP_009725307.1|2|T|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 15960 . C T 444 PASS DP=138;AF=0.137681;SB=0;DP4=99,19,17,3;EFF=SYNONYMOUS_CODING(LOW|SILENT|gcC/gcT|A5232|7096|ORF1ab|protein_coding|CODING|GU280_gp01|2|T),SYNONYMOUS_CODING(LOW|SILENT|gcC/gcT|A840|931|ORF1ab|protein_coding|CODING|YP_009725307.1|2|T|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 16729 . C T 132 PASS DP=176;AF=0.039773;SB=13;DP4=19,150,3,4;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Ctt/Ttt|L5489F|7096|ORF1ab|protein_coding|CODING|GU280_gp01|2|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Ctt/Ttt|L165F|600|ORF1ab|protein_coding|CODING|YP_009725308.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 17747 . C T 262 PASS DP=22;AF=0.409091;SB=0;DP4=11,2,7,2;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cCt/cTt|P5828L|7096|ORF1ab|protein_coding|CODING|GU280_gp01|2|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cCt/cTt|P504L|600|ORF1ab|protein_coding|CODING|YP_009725308.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 17858 . A G 296 PASS DP=29;AF=0.37931;SB=0;DP4=6,9,5,8;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|tAt/tGt|Y5865C|7096|ORF1ab|protein_coding|CODING|GU280_gp01|2|G),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|tAt/tGt|Y541C|600|ORF1ab|protein_coding|CODING|YP_009725308.1|1|G|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 17874 . C T 323 PASS DP=24;AF=0.458333;SB=0;DP4=4,8,5,7;EFF=SYNONYMOUS_CODING(LOW|SILENT|ttC/ttT|F5870|7096|ORF1ab|protein_coding|CODING|GU280_gp01|2|T),SYNONYMOUS_CODING(LOW|SILENT|ttC/ttT|F546|600|ORF1ab|protein_coding|CODING|YP_009725308.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 18788 . C T 4324 PASS DP=147;AF=0.877551;SB=0;DP4=6,6,62,72;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aCa/aTa|T6175I|7096|ORF1ab|protein_coding|CODING|GU280_gp01|2|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aCa/aTa|T250I|526|ORF1ab|protein_coding|CODING|YP_009725309.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 19044 . T TA 70 PASS DP=195;AF=0.015385;SB=0;DP4=145,47,3,0;INDEL;HRUN=3;EFF=FRAME_SHIFT(HIGH||gct/Agct|A6262S?|7096|ORF1ab|protein_coding|CODING|GU280_gp01|2|TA|INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||gct/Agct|A337S?|526|ORF1ab|protein_coding|CODING|YP_009725309.1|1|TA|WARNING_TRANSCRIPT_NO_START_CODON&INFO_REALIGN_3_PRIME);LOF=(ORF1ab|GU280_gp01|28|0.07) -NC_045512.2 19684 . G T 572 PASS DP=167;AF=0.143713;SB=0;DP4=123,19,22,3;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gta/Tta|V6474L|7096|ORF1ab|protein_coding|CODING|GU280_gp01|2|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gta/Tta|V22L|345|ORF1ab|protein_coding|CODING|YP_009725310.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 20209 . AGTAGAAATT A 29357 PASS DP=921;AF=0.972856;SB=24;DP4=18,7,825,71;INDEL;HRUN=1;EFF=CODON_CHANGE_PLUS_CODON_DELETION(MODERATE||agtagaaattta/ata|SRNL6649I|7096|ORF1ab|protein_coding|CODING|GU280_gp01|2|A),CODON_CHANGE_PLUS_CODON_DELETION(MODERATE||agtagaaattta/ata|SRNL197I|345|ORF1ab|protein_coding|CODING|YP_009725310.1|1|A|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 20287 . G A 208 sb_fdr DP=3375;AF=0.007704;SB=228;DP4=2813,531,0,29;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gaa/Aaa|E6675K|7096|ORF1ab|protein_coding|CODING|GU280_gp01|2|A),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gaa/Aaa|E223K|345|ORF1ab|protein_coding|CODING|YP_009725310.1|1|A|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 20298 . A T 253 sb_fdr DP=4476;AF=0.008266;SB=273;DP4=3504,927,1,43;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aaA/aaT|K6678N|7096|ORF1ab|protein_coding|CODING|GU280_gp01|2|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aaA/aaT|K226N|345|ORF1ab|protein_coding|CODING|YP_009725310.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 20360 . G T 112 sb_fdr DP=5879;AF=0.006464;SB=142;DP4=2816,2990,3,63;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gGt/gTt|G6699V|7096|ORF1ab|protein_coding|CODING|GU280_gp01|2|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gGt/gTt|G247V|345|ORF1ab|protein_coding|CODING|YP_009725310.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 20868 . T G 599 sb_fdr DP=31487;AF=0.004097;SB=1401;DP4=27368,3909,2,161;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|ttT/ttG|F6868L|7096|ORF1ab|protein_coding|CODING|GU280_gp01|2|G),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|ttT/ttG|F70L|297|ORF1ab|protein_coding|CODING|YP_009725311.1|1|G|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 20876 . G T 250 sb_fdr DP=33433;AF=0.002064;SB=560;DP4=27957,5364,13,90;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gGt/gTt|G6871V|7096|ORF1ab|protein_coding|CODING|GU280_gp01|2|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gGt/gTt|G73V|297|ORF1ab|protein_coding|CODING|YP_009725311.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 20883 . TA T 72 PASS DP=35902;AF=0.000334;SB=1;DP4=29071,6819,9,3;INDEL;HRUN=3;EFF=FRAME_SHIFT(HIGH||aaa/|K6874|7096|ORF1ab|protein_coding|CODING|GU280_gp01|2|T|INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||aaa/|K76|297|ORF1ab|protein_coding|CODING|YP_009725311.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON&INFO_REALIGN_3_PRIME);LOF=(ORF1ab|GU280_gp01|28|0.04) -NC_045512.2 20908 . G T 281 sb_fdr DP=45116;AF=0.002726;SB=1024;DP4=31656,13189,6,213;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gtt/Ttt|V6882F|7096|ORF1ab|protein_coding|CODING|GU280_gp01|2|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gtt/Ttt|V84F|297|ORF1ab|protein_coding|CODING|YP_009725311.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 20944 . G GA 202 PASS DP=47280;AF=0.000635;SB=55;DP4=30717,17296,30,0;INDEL;HRUN=1;EFF=FRAME_SHIFT(HIGH||gtc/gAtc|V6894D?|7096|ORF1ab|protein_coding|CODING|GU280_gp01|2|GA),FRAME_SHIFT(HIGH||gtc/gAtc|V96D?|297|ORF1ab|protein_coding|CODING|YP_009725311.1|1|GA|WARNING_TRANSCRIPT_NO_START_CODON);LOF=(ORF1ab|GU280_gp01|28|0.04) -NC_045512.2 20956 . C T 168 sb_fdr DP=45438;AF=0.002135;SB=276;DP4=26067,19195,123,1;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Ctt/Ttt|L6898F|7096|ORF1ab|protein_coding|CODING|GU280_gp01|2|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Ctt/Ttt|L100F|297|ORF1ab|protein_coding|CODING|YP_009725311.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 20982 . T TA 73 PASS DP=40146;AF=0.000349;SB=51;DP4=17187,23057,14,0;INDEL;HRUN=2;EFF=FRAME_SHIFT(HIGH||tca/Atca|S6907I?|7096|ORF1ab|protein_coding|CODING|GU280_gp01|2|TA),FRAME_SHIFT(HIGH||tca/Atca|S109I?|297|ORF1ab|protein_coding|CODING|YP_009725311.1|1|TA|WARNING_TRANSCRIPT_NO_START_CODON);LOF=(ORF1ab|GU280_gp01|28|0.04) -NC_045512.2 20990 . TGA T 397 PASS DP=38860;AF=0.001107;SB=169;DP4=14301,24944,42,1;INDEL;HRUN=1;EFF=FRAME_SHIFT(HIGH||ttgatt/|LI6909|7096|ORF1ab|protein_coding|CODING|GU280_gp01|2|T),FRAME_SHIFT(HIGH||ttgatt/|LI111|297|ORF1ab|protein_coding|CODING|YP_009725311.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON);LOF=(ORF1ab|GU280_gp01|28|0.04) -NC_045512.2 20997 . T G 385 PASS DP=36023;AF=0.003054;SB=506;DP4=10049,25768,139,25;EFF=SYNONYMOUS_CODING(LOW|SILENT|ggT/ggG|G6911|7096|ORF1ab|protein_coding|CODING|GU280_gp01|2|G),SYNONYMOUS_CODING(LOW|SILENT|ggT/ggG|G113|297|ORF1ab|protein_coding|CODING|YP_009725311.1|1|G|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 21029 . G T 192 PASS DP=31057;AF=0.001835;SB=517;DP4=4515,26363,109,51;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|tGg/tTg|W6922L|7096|ORF1ab|protein_coding|CODING|GU280_gp01|2|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|tGg/tTg|W124L|297|ORF1ab|protein_coding|CODING|YP_009725311.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 21093 . TA T 73 PASS DP=16419;AF=0.000548;SB=0;DP4=822,15597,0,9;INDEL;HRUN=3;EFF=FRAME_SHIFT(HIGH||aaa/|K6944|7096|ORF1ab|protein_coding|CODING|GU280_gp01|2|T|INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||aaa/|K146|297|ORF1ab|protein_coding|CODING|YP_009725311.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON&INFO_REALIGN_3_PRIME);LOF=(ORF1ab|GU280_gp01|28|0.04) -NC_045512.2 21134 . A G 578 PASS DP=8320;AF=0.007091;SB=4;DP4=342,7908,4,56;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cAa/cGa|Q6957R|7096|ORF1ab|protein_coding|CODING|GU280_gp01|2|G),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cAa/cGa|Q159R|297|ORF1ab|protein_coding|CODING|YP_009725311.1|1|G|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 21526 . A T 203 PASS DP=1808;AF=0.013827;SB=41;DP4=1233,532,17,26;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Att/Ttt|I7088F|7096|ORF1ab|protein_coding|CODING|GU280_gp01|2|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Att/Ttt|I290F|297|ORF1ab|protein_coding|CODING|YP_009725311.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 21578 . G T 132 PASS DP=2009;AF=0.009457;SB=26;DP4=691,1282,3,29;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gtt/Ttt|V6F|1273|S|protein_coding|CODING|GU280_gp02|1|T) -NC_045512.2 21714 . CT C 75 PASS DP=674;AF=0.005935;SB=4;DP4=79,613,1,3;INDEL;HRUN=1;EFF=FRAME_SHIFT(HIGH||act/|T51|1273|S|protein_coding|CODING|GU280_gp02|1|C) -NC_045512.2 22599 . G A 117 sb_fdr DP=30861;AF=0.001588;SB=514;DP4=24027,6728,1,81;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aGa/aAa|R346K|1273|S|protein_coding|CODING|GU280_gp02|1|A) -NC_045512.2 22689 . C T 104 sb_fdr DP=44749;AF=0.001453;SB=379;DP4=27213,17310,3,104;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aCt/aTt|T376I|1273|S|protein_coding|CODING|GU280_gp02|1|T) -NC_045512.2 22696 . GT G 113 PASS DP=44146;AF=0.000589;SB=57;DP4=26574,17974,26,0;INDEL;HRUN=1;EFF=FRAME_SHIFT(HIGH||tgt/|C379|1273|S|protein_coding|CODING|GU280_gp02|1|G);LOF=(S|GU280_gp02|1|1.00) -NC_045512.2 22703 . G GA 67 PASS DP=43227;AF=0.000324;SB=32;DP4=24542,18782,14,0;INDEL;HRUN=2;EFF=FRAME_SHIFT(HIGH||gga/gAga|G381E?|1273|S|protein_coding|CODING|GU280_gp02|1|GA);LOF=(S|GU280_gp02|1|1.00) -NC_045512.2 22705 . A G 208 sb_fdr DP=42705;AF=0.002201;SB=292;DP4=23176,19258,151,8;EFF=SYNONYMOUS_CODING(LOW|SILENT|ggA/ggG|G381|1273|S|protein_coding|CODING|GU280_gp02|1|G) -NC_045512.2 22710 . CT C 518 PASS DP=39990;AF=0.001525;SB=179;DP4=20325,19756,61,0;INDEL;HRUN=1;EFF=FRAME_SHIFT(HIGH||tct/|S383|1273|S|protein_coding|CODING|GU280_gp02|1|C);LOF=(S|GU280_gp02|1|1.00) -NC_045512.2 22712 . C T 673 sb_fdr DP=40029;AF=0.003547;SB=476;DP4=19815,19984,164,1;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Cct/Tct|P384S|1273|S|protein_coding|CODING|GU280_gp02|1|T) -NC_045512.2 22718 . A AG 80 PASS DP=45586;AF=0.000373;SB=13;DP4=19101,26602,3,14;INDEL;HRUN=3;EFF=FRAME_SHIFT(HIGH||aaa/aGaa|K386R?|1273|S|protein_coding|CODING|GU280_gp02|1|AG);LOF=(S|GU280_gp02|1|1.00) -NC_045512.2 22718 . A AT 148 PASS DP=45586;AF=0.000592;SB=63;DP4=19101,26602,0,27;INDEL;HRUN=3;EFF=FRAME_SHIFT(HIGH||aaa/aTaa|K386I?|1273|S|protein_coding|CODING|GU280_gp02|1|AT);LOF=(S|GU280_gp02|1|1.00) -NC_045512.2 22719 . A AG 141 PASS DP=45718;AF=0.000503;SB=52;DP4=19071,26637,0,23;INDEL;HRUN=3;EFF=FRAME_SHIFT(HIGH||aaa/aaGa|K386K?|1273|S|protein_coding|CODING|GU280_gp02|1|AG);LOF=(S|GU280_gp02|1|1.00) -NC_045512.2 22721 . T A 281 sb_fdr DP=46299;AF=0.002657;SB=496;DP4=18767,27328,170,13;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Tta/Ata|L387I|1273|S|protein_coding|CODING|GU280_gp02|1|A) -NC_045512.2 22725 . A T 386 sb_fdr DP=46927;AF=0.002536;SB=283;DP4=18351,28370,4,159;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aAt/aTt|N388I|1273|S|protein_coding|CODING|GU280_gp02|1|T) -NC_045512.2 22730 . CT C 111 PASS DP=47213;AF=0.000402;SB=37;DP4=17989,29386,0,19;INDEL;HRUN=1;EFF=FRAME_SHIFT(HIGH||ctc/|L390|1273|S|protein_coding|CODING|GU280_gp02|1|C);LOF=(S|GU280_gp02|1|1.00) -NC_045512.2 22747 . C T 143 sb_fdr DP=49590;AF=0.001795;SB=375;DP4=17334,32087,98,5;EFF=SYNONYMOUS_CODING(LOW|SILENT|gtC/gtT|V395|1273|S|protein_coding|CODING|GU280_gp02|1|T) -NC_045512.2 22806 . C A 87 sb_fdr DP=37559;AF=0.001118;SB=573;DP4=9268,28166,103,3;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aCt/aAt|T415N|1273|S|protein_coding|CODING|GU280_gp02|1|A) -NC_045512.2 22810 . A G 685 sb_fdr DP=36564;AF=0.006673;SB=1972;DP4=8363,27831,329,5;EFF=SYNONYMOUS_CODING(LOW|SILENT|ggA/ggG|G416|1273|S|protein_coding|CODING|GU280_gp02|1|G) -NC_045512.2 22846 . T A 794 sb_fdr DP=24191;AF=0.005085;SB=1162;DP4=3061,20971,139,4;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gaT/gaA|D428E|1273|S|protein_coding|CODING|GU280_gp02|1|A) -NC_045512.2 23251 . AC A 78 PASS DP=31241;AF=0.000544;SB=127;DP4=25676,5549,0,17;INDEL;HRUN=1;EFF=FRAME_SHIFT(HIGH||caa/|Q564|1273|S|protein_coding|CODING|GU280_gp02|1|A);LOF=(S|GU280_gp02|1|1.00) -NC_045512.2 23262 . G A 510 sb_fdr DP=32587;AF=0.002946;SB=799;DP4=26279,6161,1,114;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aGa/aAa|R567K|1273|S|protein_coding|CODING|GU280_gp02|1|A) -NC_045512.2 23263 . A G 141 sb_fdr DP=33270;AF=0.001593;SB=369;DP4=26432,6747,5,63;EFF=SYNONYMOUS_CODING(LOW|SILENT|agA/agG|R567|1273|S|protein_coding|CODING|GU280_gp02|1|G) -NC_045512.2 23269 . T C 8067 PASS DP=34669;AF=0.018028;SB=9;DP4=26501,7435,535,128;EFF=SYNONYMOUS_CODING(LOW|SILENT|atT/atC|I569|1273|S|protein_coding|CODING|GU280_gp02|1|C) -NC_045512.2 23280 . C A 731 sb_fdr DP=36362;AF=0.00473;SB=304;DP4=26586,9489,257,2;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aCt/aAt|T573N|1273|S|protein_coding|CODING|GU280_gp02|1|A) -NC_045512.2 23297 . C T 271 sb_fdr DP=34195;AF=0.003831;SB=263;DP4=22836,11142,164,1;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Cca/Tca|P579S|1273|S|protein_coding|CODING|GU280_gp02|1|T) -NC_045512.2 23299 . A C 472 sb_fdr DP=33710;AF=0.002848;SB=192;DP4=22387,11138,135,3;EFF=SYNONYMOUS_CODING(LOW|SILENT|ccA/ccC|P579|1273|S|protein_coding|CODING|GU280_gp02|1|C) -NC_045512.2 23310 . AG A 279 PASS DP=34611;AF=0.000953;SB=134;DP4=21073,13568,0,33;INDEL;HRUN=1;EFF=FRAME_SHIFT(HIGH||gag/|E583|1273|S|protein_coding|CODING|GU280_gp02|1|A);LOF=(S|GU280_gp02|1|1.00) -NC_045512.2 23311 . G A 288 sb_fdr DP=34858;AF=0.002496;SB=390;DP4=21013,13669,2,105;EFF=SYNONYMOUS_CODING(LOW|SILENT|gaG/gaA|E583|1273|S|protein_coding|CODING|GU280_gp02|1|A) -NC_045512.2 23314 . TC T 324 PASS DP=34698;AF=0.001614;SB=26;DP4=20426,14472,44,12;INDEL;HRUN=1;EFF=FRAME_SHIFT(HIGH||ctt/|L585|1273|S|protein_coding|CODING|GU280_gp02|1|T);LOF=(S|GU280_gp02|1|1.00) -NC_045512.2 23315 . C T 1344 sb_fdr DP=34808;AF=0.0079;SB=299;DP4=19943,14452,288,42;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Ctt/Ttt|L585F|1273|S|protein_coding|CODING|GU280_gp02|1|T) -NC_045512.2 23338 . T G 1437 sb_fdr DP=37322;AF=0.007234;SB=1204;DP4=18321,18550,1,415;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|ttT/ttG|F592L|1273|S|protein_coding|CODING|GU280_gp02|1|G) -NC_045512.2 23359 . A AT 100 PASS DP=34134;AF=0.000498;SB=2;DP4=12901,21656,5,12;INDEL;HRUN=2;EFF=FRAME_SHIFT(HIGH||cca/Tcca|P600S?|1273|S|protein_coding|CODING|GU280_gp02|1|AT);LOF=(S|GU280_gp02|1|1.00) -NC_045512.2 23360 . C CT 83 PASS DP=33267;AF=0.000421;SB=7;DP4=12485,21911,8,6;INDEL;HRUN=2;EFF=FRAME_SHIFT(HIGH||cca/cTca|P600L?|1273|S|protein_coding|CODING|GU280_gp02|1|CT);LOF=(S|GU280_gp02|1|1.00) -NC_045512.2 23364 . G A 113 sb_fdr DP=33221;AF=0.002378;SB=156;DP4=10747,22299,3,116;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gGa/gAa|G601E|1273|S|protein_coding|CODING|GU280_gp02|1|A) -NC_045512.2 23367 . C CG 88 PASS DP=36508;AF=0.000466;SB=10;DP4=9567,27089,1,16;INDEL;HRUN=3;EFF=FRAME_SHIFT(HIGH||aca/acGa|T602T?|1273|S|protein_coding|CODING|GU280_gp02|1|CG);LOF=(S|GU280_gp02|1|1.00) -NC_045512.2 23367 . C CT 107 PASS DP=36508;AF=0.000548;SB=14;DP4=9567,27089,1,19;INDEL;HRUN=3;EFF=FRAME_SHIFT(HIGH||aca/acTa|T602T?|1273|S|protein_coding|CODING|GU280_gp02|1|CT);LOF=(S|GU280_gp02|1|1.00) -NC_045512.2 23370 . A AC 71 PASS DP=37817;AF=0.00037;SB=15;DP4=9247,28683,0,14;INDEL;HRUN=1;EFF=FRAME_SHIFT(HIGH||aat/aaCt|N603N?|1273|S|protein_coding|CODING|GU280_gp02|1|AC);LOF=(S|GU280_gp02|1|1.00) -NC_045512.2 23376 . C T 203 sb_fdr DP=37684;AF=0.001725;SB=426;DP4=8482,29112,71,2;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|tCt/tTt|S605F|1273|S|protein_coding|CODING|GU280_gp02|1|T) -NC_045512.2 23385 . T G 91 PASS DP=36445;AF=0.001015;SB=11;DP4=8038,28224,44,111;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gTt/gGt|V608G|1273|S|protein_coding|CODING|GU280_gp02|1|G) -NC_045512.2 23403 . A G 512 PASS DP=34976;AF=0.002573;SB=0;DP4=7392,27461,21,79;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gAt/gGt|D614G|1273|S|protein_coding|CODING|GU280_gp02|1|G) -NC_045512.2 23996 . C A 136 sb_fdr DP=1202;AF=0.016639;SB=295;DP4=336,779,63,1;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Cca/Aca|P812T|1273|S|protein_coding|CODING|GU280_gp02|1|A) -NC_045512.2 25563 . G T 1961 PASS DP=67;AF=0.850746;SB=0;DP4=0,6,4,57;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|caG/caT|Q57H|275|ORF3a|protein_coding|CODING|GU280_gp03|1|T) -NC_045512.2 28077 . G C 164 PASS DP=99;AF=0.080808;SB=1;DP4=21,68,1,8;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gtg/Ctg|V62L|121|ORF8|protein_coding|CODING|GU280_gp09|1|C) -NC_045512.2 28214 . C CT 71 PASS DP=118;AF=0.025424;SB=0;DP4=98,17,3,0;INDEL;HRUN=5;EFF=FRAME_SHIFT(HIGH||tta/ttTa|L109F?|121|ORF8|protein_coding|CODING|GU280_gp09|1|CT|INFO_REALIGN_3_PRIME);LOF=(ORF8|GU280_gp09|1|1.00) -NC_045512.2 28253 . C T 168 PASS DP=113;AF=0.088496;SB=0;DP4=75,26,9,3;EFF=SYNONYMOUS_CODING(LOW|SILENT|ttC/ttT|F120|121|ORF8|protein_coding|CODING|GU280_gp09|1|T) -NC_045512.2 29700 . A G 331 PASS DP=148;AF=0.101351;SB=0;DP4=45,85,6,11 diff --git a/workflows/sars-cov-2-variant-calling/sars-cov-2-pe-illumina-wgs-variant-calling/.dockstore.yml b/workflows/sars-cov-2-variant-calling/sars-cov-2-pe-illumina-wgs-variant-calling/.dockstore.yml index 203b59c5a..c13eb3be8 100644 --- a/workflows/sars-cov-2-variant-calling/sars-cov-2-pe-illumina-wgs-variant-calling/.dockstore.yml +++ b/workflows/sars-cov-2-variant-calling/sars-cov-2-pe-illumina-wgs-variant-calling/.dockstore.yml @@ -1,7 +1,11 @@ version: 1.2 workflows: - name: 'COVID-19-PE-WGS-ILLUMINA' - primaryDescriptorPath: /pe-wgs-variation.ga subclass: Galaxy + publish: true + primaryDescriptorPath: /pe-wgs-variation.ga testParameterFiles: - - /pe-wgs-variation-test.yml + - /pe-wgs-variation-tests.yml + authors: + - name: Wolfgang Maier + orcid: 0000-0002-9464-6640 diff --git a/workflows/sars-cov-2-variant-calling/sars-cov-2-pe-illumina-wgs-variant-calling/CHANGELOG.md b/workflows/sars-cov-2-variant-calling/sars-cov-2-pe-illumina-wgs-variant-calling/CHANGELOG.md index ce9daedbc..79dd9e0ae 100644 --- a/workflows/sars-cov-2-variant-calling/sars-cov-2-pe-illumina-wgs-variant-calling/CHANGELOG.md +++ b/workflows/sars-cov-2-variant-calling/sars-cov-2-pe-illumina-wgs-variant-calling/CHANGELOG.md @@ -1,5 +1,10 @@ # Changelog +## [0.2.4] 2023-11-20 + +- Fix author in dockstore +- Use zenodo link instead of googleapis for fastq + ## [0.2.3] 2022-02-04 ### Automatic update diff --git a/workflows/sars-cov-2-variant-calling/sars-cov-2-pe-illumina-wgs-variant-calling/pe-wgs-variation-test.yml b/workflows/sars-cov-2-variant-calling/sars-cov-2-pe-illumina-wgs-variant-calling/pe-wgs-variation-tests.yml similarity index 76% rename from workflows/sars-cov-2-variant-calling/sars-cov-2-pe-illumina-wgs-variant-calling/pe-wgs-variation-test.yml rename to workflows/sars-cov-2-variant-calling/sars-cov-2-pe-illumina-wgs-variant-calling/pe-wgs-variation-tests.yml index e1d383a51..843aab649 100644 --- a/workflows/sars-cov-2-variant-calling/sars-cov-2-pe-illumina-wgs-variant-calling/pe-wgs-variation-test.yml +++ b/workflows/sars-cov-2-variant-calling/sars-cov-2-pe-illumina-wgs-variant-calling/pe-wgs-variation-tests.yml @@ -13,10 +13,10 @@ elements: - identifier: forward class: File - location: "https://storage.googleapis.com/nih-sequence-read-archive/sra-src/SRR11578257/VIC818_R1.fq.gz" + location: "https://zenodo.org/records/10174466/files/SRR11578257_R1.fastq.gz?download=1" - identifier: reverse class: File - location: "https://storage.googleapis.com/nih-sequence-read-archive/sra-src/SRR11578257/VIC818_R2.fq.gz" + location: "https://zenodo.org/records/10174466/files/SRR11578257_R2.fastq.gz?download=1" outputs: annotated_variants: attributes: {} diff --git a/workflows/sars-cov-2-variant-calling/sars-cov-2-pe-illumina-wgs-variant-calling/pe-wgs-variation.ga b/workflows/sars-cov-2-variant-calling/sars-cov-2-pe-illumina-wgs-variant-calling/pe-wgs-variation.ga index b4877822e..9a814598b 100644 --- a/workflows/sars-cov-2-variant-calling/sars-cov-2-pe-illumina-wgs-variant-calling/pe-wgs-variation.ga +++ b/workflows/sars-cov-2-variant-calling/sars-cov-2-pe-illumina-wgs-variant-calling/pe-wgs-variation.ga @@ -11,7 +11,7 @@ "format-version": "0.1", "license": "MIT", "name": "COVID-19: variation analysis on WGS PE data", - "release": "0.2.3", + "release": "0.2.4", "steps": { "0": { "annotation": "Illumina reads with fastqsanger encoding", diff --git a/workflows/sars-cov-2-variant-calling/sars-cov-2-se-illumina-wgs-variant-calling/.dockstore.yml b/workflows/sars-cov-2-variant-calling/sars-cov-2-se-illumina-wgs-variant-calling/.dockstore.yml index 08471be94..1bbc1d902 100644 --- a/workflows/sars-cov-2-variant-calling/sars-cov-2-se-illumina-wgs-variant-calling/.dockstore.yml +++ b/workflows/sars-cov-2-variant-calling/sars-cov-2-se-illumina-wgs-variant-calling/.dockstore.yml @@ -1,7 +1,11 @@ version: 1.2 workflows: - name: 'COVID-19-SE-WGS-ILLUMINA' - primaryDescriptorPath: /se-wgs-variation.ga subclass: Galaxy + publish: true + primaryDescriptorPath: /se-wgs-variation.ga testParameterFiles: - - /se-wgs-variation-test.yml + - /se-wgs-variation-tests.yml + authors: + - name: Wolfgang Maier + orcid: 0000-0002-9464-6640 diff --git a/workflows/sars-cov-2-variant-calling/sars-cov-2-se-illumina-wgs-variant-calling/CHANGELOG.md b/workflows/sars-cov-2-variant-calling/sars-cov-2-se-illumina-wgs-variant-calling/CHANGELOG.md index 547051c78..d69dc9c15 100644 --- a/workflows/sars-cov-2-variant-calling/sars-cov-2-se-illumina-wgs-variant-calling/CHANGELOG.md +++ b/workflows/sars-cov-2-variant-calling/sars-cov-2-se-illumina-wgs-variant-calling/CHANGELOG.md @@ -1,5 +1,21 @@ # Changelog +## [0.1.5] 2024-03-05 + +### Automatic update +- `toolshed.g2.bx.psu.edu/repos/iuc/fastp/fastp/0.20.1+galaxy0` was updated to `toolshed.g2.bx.psu.edu/repos/iuc/fastp/fastp/0.23.4+galaxy0` +- `toolshed.g2.bx.psu.edu/repos/devteam/bowtie2/bowtie2/2.3.4.2` was updated to `toolshed.g2.bx.psu.edu/repos/devteam/bowtie2/bowtie2/2.5.3+galaxy0` +- `toolshed.g2.bx.psu.edu/repos/devteam/picard/picard_MarkDuplicates/2.18.2.2` was updated to `toolshed.g2.bx.psu.edu/repos/devteam/picard/picard_MarkDuplicates/3.1.1.0` +- `toolshed.g2.bx.psu.edu/repos/iuc/multiqc/multiqc/1.8+galaxy1` was updated to `toolshed.g2.bx.psu.edu/repos/iuc/multiqc/multiqc/1.11+galaxy1` +- `toolshed.g2.bx.psu.edu/repos/iuc/lofreq_indelqual/lofreq_indelqual/2.1.5+galaxy0` was updated to `toolshed.g2.bx.psu.edu/repos/iuc/lofreq_indelqual/lofreq_indelqual/2.1.5+galaxy1` +- `toolshed.g2.bx.psu.edu/repos/iuc/lofreq_call/lofreq_call/2.1.5+galaxy0` was updated to `toolshed.g2.bx.psu.edu/repos/iuc/lofreq_call/lofreq_call/2.1.5+galaxy2` + +## [0.1.4] 2023-11-20 + +- Fix author in dockstore +- Fix input fastq URL for test +- Update output test + ## [0.1.3] 2021-12-13 ### Added diff --git a/workflows/sars-cov-2-variant-calling/sars-cov-2-se-illumina-wgs-variant-calling/se-wgs-variation-test.yml b/workflows/sars-cov-2-variant-calling/sars-cov-2-se-illumina-wgs-variant-calling/se-wgs-variation-test.yml deleted file mode 100644 index 07327e6ad..000000000 --- a/workflows/sars-cov-2-variant-calling/sars-cov-2-se-illumina-wgs-variant-calling/se-wgs-variation-test.yml +++ /dev/null @@ -1,20 +0,0 @@ -- doc: Test workflow execution using paired end illumina accession - job: - NC_045512.2 FASTA sequence of SARS-CoV-2: - class: File - location: https://zenodo.org/record/4555735/files/NC_045512.2_reference.fasta?download=1 - Single End Collection: - class: Collection - collection_type: 'list' - elements: - - identifier: SRR11605118 - class: File - location: "https://sra-pub-sars-cov2.s3.amazonaws.com/sra-src/SRR11605118/VA-DCLS-0041_SC2_R1.fastq.gz" - outputs: - annotated_variants: - attributes: {} - element_tests: - SRR11605118: - path: test-data/final_snpeff_annotated_variants.vcf - compare: diff - lines_diff: 6 diff --git a/workflows/sars-cov-2-variant-calling/sars-cov-2-se-illumina-wgs-variant-calling/se-wgs-variation-tests.yml b/workflows/sars-cov-2-variant-calling/sars-cov-2-se-illumina-wgs-variant-calling/se-wgs-variation-tests.yml new file mode 100644 index 000000000..c2567597c --- /dev/null +++ b/workflows/sars-cov-2-variant-calling/sars-cov-2-se-illumina-wgs-variant-calling/se-wgs-variation-tests.yml @@ -0,0 +1,26 @@ +- doc: Test workflow execution using paired end illumina accession + job: + NC_045512.2 FASTA sequence of SARS-CoV-2: + class: File + location: https://zenodo.org/record/4555735/files/NC_045512.2_reference.fasta?download=1 + Single End Collection: + class: Collection + collection_type: 'list' + elements: + - identifier: SRR11605118 + class: File + location: "https://www.be-md.ncbi.nlm.nih.gov/Traces/sra-reads-be/fastq?acc=SRR11605118" + outputs: + annotated_variants: + attributes: {} + element_tests: + SRR11605118: + asserts: + has_line: + line: "##fileformat=VCFv4.0" + has_line: + line: "#CHROM POS ID REF ALT QUAL FILTER INFO" + has_text_matching: + expression: "NC_045512.2\t241\t.\tC\tT\t[0-9.]*\tPASS" + has_text_matching: + expression: "NC_045512.2\t16111\t.\tC\tT\t[0-9.]*\tPASS\tDP=[0-9]*;AF=0.[89][0-9]*;SB=0;DP4=[0-9]*,[0-9]*,[0-9]*,[0-9]*;EFF=SYNONYMOUS_CODING\\(LOW|SILENT|Cta/Tta|L5283|7096|ORF1ab|protein_coding|CODING|GU280_gp01|2|T\\),SYNONYMOUS_CODING\\(LOW|SILENT|Cta/Tta|L891|931|ORF1ab|protein_coding|CODING|YP_009725307.1|2|T|WARNING_TRANSCRIPT_NO_START_CODON\\)" diff --git a/workflows/sars-cov-2-variant-calling/sars-cov-2-se-illumina-wgs-variant-calling/se-wgs-variation.ga b/workflows/sars-cov-2-variant-calling/sars-cov-2-se-illumina-wgs-variant-calling/se-wgs-variation.ga index a1b5b3e0d..234a49ffd 100644 --- a/workflows/sars-cov-2-variant-calling/sars-cov-2-se-illumina-wgs-variant-calling/se-wgs-variation.ga +++ b/workflows/sars-cov-2-variant-calling/sars-cov-2-se-illumina-wgs-variant-calling/se-wgs-variation.ga @@ -11,7 +11,7 @@ "format-version": "0.1", "license": "MIT", "name": "COVID-19: variation analysis on WGS SE data", - "release": "0.1.3", + "release": "0.1.5", "steps": { "0": { "annotation": "Illumina reads with fastqsanger encoding", @@ -29,20 +29,15 @@ "name": "Input dataset collection", "outputs": [], "position": { - "bottom": 481.8125, - "height": 101, - "left": 353.5625, - "right": 553.5625, - "top": 380.8125, - "width": 200, - "x": 353.5625, - "y": 380.8125 + "left": 0.0, + "top": 189.96875 }, "tool_id": null, - "tool_state": "{\"optional\": false, \"format\": [\"fastqsanger\", \"fastqsanger.gz\"], \"collection_type\": \"list\"}", + "tool_state": "{\"optional\": false, \"format\": [\"fastqsanger\", \"fastqsanger.gz\"], \"tag\": null, \"collection_type\": \"list\"}", "tool_version": null, "type": "data_collection_input", "uuid": "fac0f79d-54bd-4c26-b18e-2804067a6522", + "when": null, "workflow_outputs": [] }, "1": { @@ -61,25 +56,20 @@ "name": "Input dataset", "outputs": [], "position": { - "bottom": 893.828125, - "height": 101, - "left": 449.546875, - "right": 649.546875, - "top": 792.828125, - "width": 200, - "x": 449.546875, - "y": 792.828125 + "left": 95.984375, + "top": 601.984375 }, "tool_id": null, - "tool_state": "{\"optional\": false, \"format\": [\"fasta\", \"fasta.gz\"]}", + "tool_state": "{\"optional\": false, \"format\": [\"fasta\", \"fasta.gz\"], \"tag\": null}", "tool_version": null, "type": "data_input", "uuid": "c9a062f4-3154-47de-b482-d8168a70f939", + "when": null, "workflow_outputs": [] }, "2": { "annotation": "", - "content_id": "toolshed.g2.bx.psu.edu/repos/iuc/fastp/fastp/0.20.1+galaxy0", + "content_id": "toolshed.g2.bx.psu.edu/repos/iuc/fastp/fastp/0.23.4+galaxy0", "errors": null, "id": 2, "input_connections": { @@ -88,7 +78,12 @@ "output_name": "output" } }, - "inputs": [], + "inputs": [ + { + "description": "runtime parameter for tool fastp", + "name": "single_paired" + } + ], "label": null, "name": "fastp", "outputs": [ @@ -106,14 +101,8 @@ } ], "position": { - "bottom": 402.84375, - "height": 212, - "left": 591.09375, - "right": 791.09375, - "top": 190.84375, - "width": 200, - "x": 591.09375, - "y": 190.84375 + "left": 237.53125, + "top": 0.0 }, "post_job_actions": { "HideDatasetActionreport_json": { @@ -129,17 +118,18 @@ "output_name": "out1" } }, - "tool_id": "toolshed.g2.bx.psu.edu/repos/iuc/fastp/fastp/0.20.1+galaxy0", + "tool_id": "toolshed.g2.bx.psu.edu/repos/iuc/fastp/fastp/0.23.4+galaxy0", "tool_shed_repository": { - "changeset_revision": "dbf9c561ef29", + "changeset_revision": "c59d48774d03", "name": "fastp", "owner": "iuc", "tool_shed": "toolshed.g2.bx.psu.edu" }, - "tool_state": "{\"filter_options\": {\"quality_filtering_options\": {\"disable_quality_filtering\": \"false\", \"qualified_quality_phred\": null, \"unqualified_percent_limit\": null, \"n_base_limit\": null}, \"length_filtering_options\": {\"disable_length_filtering\": \"false\", \"length_required\": null, \"length_limit\": null}, \"low_complexity_filter\": {\"enable_low_complexity_filter\": \"false\", \"complexity_threshold\": null}}, \"output_options\": {\"report_html\": \"true\", \"report_json\": \"true\"}, \"overrepresented_sequence_analysis\": {\"overrepresentation_analysis\": \"false\", \"overrepresentation_sampling\": null}, \"read_mod_options\": {\"polyg_tail_trimming\": {\"trimming_select\": \"\", \"__current_case__\": 1, \"poly_g_min_len\": null}, \"polyx_tail_trimming\": {\"polyx_trimming_select\": \"\", \"__current_case__\": 1}, \"umi_processing\": {\"umi\": \"false\", \"umi_loc\": \"\", \"umi_len\": null, \"umi_prefix\": \"\"}, \"cutting_by_quality_options\": {\"cut_by_quality5\": \"false\", \"cut_by_quality3\": \"false\", \"cut_window_size\": null, \"cut_mean_quality\": null}, \"base_correction_options\": {\"correction\": \"false\"}}, \"single_paired\": {\"single_paired_selector\": \"single\", \"__current_case__\": 0, \"in1\": {\"__class__\": \"ConnectedValue\"}, \"adapter_trimming_options\": {\"disable_adapter_trimming\": \"false\", \"adapter_sequence1\": \"\"}, \"global_trimming_options\": {\"trim_front1\": null, \"trim_tail1\": null}}, \"__page__\": null, \"__rerun_remap_job_id__\": null}", - "tool_version": "0.20.1+galaxy0", + "tool_state": "{\"filter_options\": {\"quality_filtering_options\": {\"disable_quality_filtering\": false, \"qualified_quality_phred\": null, \"unqualified_percent_limit\": null, \"n_base_limit\": null}, \"length_filtering_options\": {\"disable_length_filtering\": false, \"length_required\": null, \"length_limit\": null}, \"low_complexity_filter\": {\"enable_low_complexity_filter\": false, \"complexity_threshold\": null}}, \"output_options\": {\"report_html\": true, \"report_json\": true}, \"overrepresented_sequence_analysis\": {\"overrepresentation_analysis\": false, \"overrepresentation_sampling\": null}, \"read_mod_options\": {\"polyg_tail_trimming\": {\"trimming_select\": \"\", \"__current_case__\": 1, \"poly_g_min_len\": null}, \"polyx_tail_trimming\": {\"polyx_trimming_select\": \"\", \"__current_case__\": 1}, \"umi_processing\": {\"umi\": false, \"umi_loc\": \"\", \"umi_len\": null, \"umi_prefix\": \"\"}, \"cutting_by_quality_options\": {\"cut_by_quality5\": false, \"cut_by_quality3\": false, \"cut_window_size\": null, \"cut_mean_quality\": null}, \"base_correction_options\": {\"correction\": false}}, \"single_paired\": {\"single_paired_selector\": \"single\", \"__current_case__\": 0, \"in1\": {\"__class__\": \"ConnectedValue\"}, \"adapter_trimming_options\": {\"disable_adapter_trimming\": false, \"adapter_sequence1\": \"\"}, \"global_trimming_options\": {\"trim_front1\": null, \"trim_tail1\": null}}, \"__page__\": null, \"__rerun_remap_job_id__\": null}", + "tool_version": "0.23.4+galaxy0", "type": "tool", "uuid": "226bdff2-28d7-46ec-9a17-887d577056f6", + "when": null, "workflow_outputs": [ { "label": "preprocessed_reads", @@ -155,7 +145,7 @@ }, "3": { "annotation": "", - "content_id": "toolshed.g2.bx.psu.edu/repos/devteam/bowtie2/bowtie2/2.3.4.2", + "content_id": "toolshed.g2.bx.psu.edu/repos/devteam/bowtie2/bowtie2/2.5.3+galaxy0", "errors": null, "id": 3, "input_connections": { @@ -168,7 +158,16 @@ "output_name": "output" } }, - "inputs": [], + "inputs": [ + { + "description": "runtime parameter for tool Bowtie2", + "name": "library" + }, + { + "description": "runtime parameter for tool Bowtie2", + "name": "reference_genome" + } + ], "label": null, "name": "Bowtie2", "outputs": [ @@ -182,14 +181,8 @@ } ], "position": { - "bottom": 613.734375, - "height": 212, - "left": 829.765625, - "right": 1029.765625, - "top": 401.734375, - "width": 200, - "x": 829.765625, - "y": 401.734375 + "left": 476.203125, + "top": 210.890625 }, "post_job_actions": { "HideDatasetActionmapping_stats": { @@ -205,17 +198,18 @@ "output_name": "output" } }, - "tool_id": "toolshed.g2.bx.psu.edu/repos/devteam/bowtie2/bowtie2/2.3.4.2", + "tool_id": "toolshed.g2.bx.psu.edu/repos/devteam/bowtie2/bowtie2/2.5.3+galaxy0", "tool_shed_repository": { - "changeset_revision": "17062a0decb7", + "changeset_revision": "0d4acadabb04", "name": "bowtie2", "owner": "devteam", "tool_shed": "toolshed.g2.bx.psu.edu" }, - "tool_state": "{\"analysis_type\": {\"analysis_type_selector\": \"simple\", \"__current_case__\": 0, \"presets\": \"--very-sensitive\"}, \"library\": {\"type\": \"single\", \"__current_case__\": 0, \"input_1\": {\"__class__\": \"ConnectedValue\"}, \"unaligned_file\": \"false\", \"aligned_file\": \"false\"}, \"reference_genome\": {\"source\": \"history\", \"__current_case__\": 1, \"own_file\": {\"__class__\": \"ConnectedValue\"}}, \"rg\": {\"rg_selector\": \"set_id_auto\", \"__current_case__\": 2}, \"sam_options\": {\"sam_options_selector\": \"no\", \"__current_case__\": 1}, \"save_mapping_stats\": \"true\", \"__page__\": null, \"__rerun_remap_job_id__\": null}", - "tool_version": "2.3.4.2", + "tool_state": "{\"analysis_type\": {\"analysis_type_selector\": \"simple\", \"__current_case__\": 0, \"presets\": \"--very-sensitive\"}, \"library\": {\"type\": \"single\", \"__current_case__\": 0, \"input_1\": {\"__class__\": \"ConnectedValue\"}, \"unaligned_file\": false, \"aligned_file\": false}, \"reference_genome\": {\"source\": \"history\", \"__current_case__\": 1, \"own_file\": {\"__class__\": \"ConnectedValue\"}}, \"rg\": {\"rg_selector\": \"set_id_auto\", \"__current_case__\": 2}, \"sam_options\": {\"sam_options_selector\": \"no\", \"__current_case__\": 1}, \"save_mapping_stats\": true, \"__page__\": null, \"__rerun_remap_job_id__\": null}", + "tool_version": "2.5.3+galaxy0", "type": "tool", "uuid": "befd5b2a-7f19-48bf-9133-55dddc3791f2", + "when": null, "workflow_outputs": [ { "label": "mapped_reads", @@ -226,7 +220,7 @@ }, "4": { "annotation": "", - 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"tool_state": "{\"__input_ext\": \"bam\", \"assume_sorted\": \"true\", \"barcode_tag\": \"\", \"chromInfo\": \"/cvmfs/data.galaxyproject.org/managed/len/ucsc/?.len\", \"comments\": [], \"duplicate_scoring_strategy\": \"SUM_OF_BASE_QUALITIES\", \"inputFile\": {\"__class__\": \"ConnectedValue\"}, \"inputFile|__identifier__\": \"SRR11247078\", \"optical_duplicate_pixel_distance\": \"100\", \"read_name_regex\": \"\", \"remove_duplicates\": \"true\", \"validation_stringency\": \"LENIENT\", \"__page__\": null, \"__rerun_remap_job_id__\": null}", - "tool_version": "2.18.2.2", + "tool_state": "{\"__input_ext\": \"bam\", \"assume_sorted\": true, \"barcode_tag\": \"\", \"chromInfo\": \"/cvmfs/data.galaxyproject.org/managed/len/ucsc/?.len\", \"comments\": [], \"duplicate_scoring_strategy\": \"SUM_OF_BASE_QUALITIES\", \"inputFile\": {\"__class__\": \"ConnectedValue\"}, \"inputFile|__identifier__\": \"SRR11247078\", \"optical_duplicate_pixel_distance\": \"100\", \"read_name_regex\": \"\", \"remove_duplicates\": true, \"validation_stringency\": \"LENIENT\", \"__page__\": null, \"__rerun_remap_job_id__\": null}", + "tool_version": "3.1.1.0", "type": "tool", "uuid": "298f0df2-22f1-405d-92b3-272f73e3ef5b", + "when": null, "workflow_outputs": [ { "label": "markduplicates_stats", @@ -285,7 +279,7 @@ }, "5": { "annotation": "", - 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"changeset_revision": "354b534eeab7", + "changeset_revision": "971e07ca4456", "name": "lofreq_indelqual", "owner": "iuc", "tool_shed": "toolshed.g2.bx.psu.edu" }, "tool_state": "{\"reads\": {\"__class__\": \"ConnectedValue\"}, \"strategy\": {\"selector\": \"dindel\", \"__current_case__\": 1, \"reference_source\": {\"ref_selector\": \"history\", \"__current_case__\": 1, \"ref\": {\"__class__\": \"ConnectedValue\"}}}, \"__page__\": null, \"__rerun_remap_job_id__\": null}", - "tool_version": "2.1.5+galaxy0", + "tool_version": "2.1.5+galaxy1", "type": "tool", "uuid": "b2abc152-bdea-418f-af6b-566f83418ffb", + "when": null, "workflow_outputs": [ { "label": "realigned_deduplicated_mapped_reads_with_indel_quals", @@ -485,7 +474,7 @@ }, "8": { "annotation": "", - "content_id": "toolshed.g2.bx.psu.edu/repos/iuc/lofreq_call/lofreq_call/2.1.5+galaxy0", + "content_id": "toolshed.g2.bx.psu.edu/repos/iuc/lofreq_call/lofreq_call/2.1.5+galaxy2", "errors": null, "id": 8, "input_connections": { @@ -498,7 +487,16 @@ "output_name": "output" } }, - 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"inputs": [], + "inputs": [ + { + "description": "runtime parameter for tool Lofreq filter", + "name": "invcf" + } + ], "label": null, "name": "Lofreq filter", "outputs": [ @@ -558,27 +556,22 @@ } ], "position": { - "bottom": 915.265625, - "height": 112, - "left": 1852.078125, - "right": 2052.078125, - "top": 803.265625, - "width": 200, - "x": 1852.078125, - "y": 803.265625 + "left": 1498.515625, + "top": 612.421875 }, "post_job_actions": {}, "tool_id": "toolshed.g2.bx.psu.edu/repos/iuc/lofreq_filter/lofreq_filter/2.1.5+galaxy0", "tool_shed_repository": { - "changeset_revision": "950d1d49d678", + "changeset_revision": "7dfca164d2e3", "name": "lofreq_filter", "owner": "iuc", "tool_shed": "toolshed.g2.bx.psu.edu" }, - "tool_state": "{\"af\": {\"af_min\": \"0.0\", \"af_max\": \"0.0\"}, \"coverage\": {\"cov_min\": \"0\", \"cov_max\": \"0\"}, \"filter_by_type\": {\"keep_only\": \"\", \"__current_case__\": 0, \"qual\": {\"snvqual_filter\": {\"snvqual\": \"no\", \"__current_case__\": 0}, \"indelqual_filter\": {\"indelqual\": \"no\", \"__current_case__\": 0}}}, \"flag_or_drop\": \"--print-all\", \"invcf\": {\"__class__\": \"ConnectedValue\"}, \"sb\": {\"sb_filter\": {\"strand_bias\": \"mtc\", \"__current_case__\": 2, \"sb_alpha\": \"0.001\", \"sb_mtc\": \"fdr\", \"sb_compound\": \"true\", \"sb_indels\": \"false\"}}, \"__page__\": null, \"__rerun_remap_job_id__\": null}", + "tool_state": "{\"af\": {\"af_min\": \"0.0\", \"af_max\": \"0.0\"}, \"coverage\": {\"cov_min\": \"0\", \"cov_max\": \"0\"}, \"filter_by_type\": {\"keep_only\": \"\", \"__current_case__\": 0, \"qual\": {\"snvqual_filter\": {\"snvqual\": \"no\", \"__current_case__\": 0}, \"indelqual_filter\": {\"indelqual\": \"no\", \"__current_case__\": 0}}}, \"flag_or_drop\": \"--print-all\", \"invcf\": {\"__class__\": \"ConnectedValue\"}, \"sb\": {\"sb_filter\": {\"strand_bias\": \"mtc\", \"__current_case__\": 2, \"sb_alpha\": \"0.001\", \"sb_mtc\": \"fdr\", \"sb_compound\": true, \"sb_indels\": false}}, \"__page__\": null, \"__rerun_remap_job_id__\": null}", "tool_version": "2.1.5+galaxy0", "type": "tool", "uuid": "f9db3062-6214-4c07-a006-001277dc0be5", + "when": null, "workflow_outputs": [ { "label": "soft_filtered_variants", @@ -599,6 +592,10 @@ } }, "inputs": [ + { + "description": "runtime parameter for tool SnpEff eff:", + "name": "input" + }, { "description": "runtime parameter for tool SnpEff eff:", "name": "intervals" @@ -621,14 +618,8 @@ } ], "position": { - "bottom": 778.34375, - "height": 302, - "left": 1897.96875, - "right": 2097.96875, - "top": 476.34375, - "width": 200, - "x": 1897.96875, - "y": 476.34375 + "left": 1544.40625, + "top": 285.5 }, "post_job_actions": { "RenameDatasetActionsnpeff_output": { @@ -646,10 +637,11 @@ "owner": "iuc", "tool_shed": "toolshed.g2.bx.psu.edu" }, - "tool_state": "{\"annotations\": [\"-formatEff\", \"-classic\"], \"chr\": \"\", \"csvStats\": \"false\", \"filter\": {\"specificEffects\": \"no\", \"__current_case__\": 0}, \"filterOut\": [\"-no-downstream\", \"-no-intergenic\", \"-no-upstream\", \"-no-utr\"], \"generate_stats\": \"true\", \"genome_version\": \"NC_045512.2\", \"input\": {\"__class__\": \"ConnectedValue\"}, \"inputFormat\": \"vcf\", \"intervals\": {\"__class__\": \"RuntimeValue\"}, \"noLog\": \"true\", \"offset\": \"default\", \"outputConditional\": {\"outputFormat\": \"vcf\", \"__current_case__\": 0}, \"transcripts\": {\"__class__\": \"RuntimeValue\"}, \"udLength\": \"0\", \"__page__\": null, \"__rerun_remap_job_id__\": null}", + "tool_state": "{\"annotations\": [\"-formatEff\", \"-classic\"], \"chr\": \"\", \"csvStats\": false, \"filter\": {\"specificEffects\": \"no\", \"__current_case__\": 0}, \"filterOut\": [\"-no-downstream\", \"-no-intergenic\", \"-no-upstream\", \"-no-utr\"], \"generate_stats\": true, \"genome_version\": \"NC_045512.2\", \"input\": {\"__class__\": \"ConnectedValue\"}, \"inputFormat\": \"vcf\", \"intervals\": {\"__class__\": \"RuntimeValue\"}, \"noLog\": true, \"offset\": \"default\", \"outputConditional\": {\"outputFormat\": \"vcf\", \"__current_case__\": 0}, \"transcripts\": {\"__class__\": \"RuntimeValue\"}, \"udLength\": \"0\", \"__page__\": null, \"__rerun_remap_job_id__\": null}", "tool_version": "4.5covid19", "type": "tool", "uuid": "6b370dfb-970b-49c2-8060-c017f50508e8", + "when": null, "workflow_outputs": [ { "label": "annotated_variants", @@ -670,4 +662,4 @@ ], "uuid": "5171bc59-590c-4e97-8db8-004f73928627", "version": 6 -} +} \ No newline at end of file diff --git a/workflows/sars-cov-2-variant-calling/sars-cov-2-se-illumina-wgs-variant-calling/test-data/final_snpeff_annotated_variants.vcf b/workflows/sars-cov-2-variant-calling/sars-cov-2-se-illumina-wgs-variant-calling/test-data/final_snpeff_annotated_variants.vcf deleted file mode 100644 index af19f22d4..000000000 --- a/workflows/sars-cov-2-variant-calling/sars-cov-2-se-illumina-wgs-variant-calling/test-data/final_snpeff_annotated_variants.vcf +++ /dev/null @@ -1,204 +0,0 @@ -##fileformat=VCFv4.0 -##fileDate=20210221 -##source=lofreq call --verbose --ref reference.fa --call-indels --min-cov 5 --max-depth 1000000 --min-bq 30 --min-alt-bq 30 --min-mq 20 --max-mq 255 --min-jq 0 --min-alt-jq 0 --def-alt-jq 0 --sig 0.0005 --bonf dynamic --no-default-filter --no-default-filter -r NC_045512.2:1-14951 -o /tmp/lofreq2_call_parallelela34bnj/0.vcf.gz reads.bam -##reference=reference.fa -##INFO= -##INFO= -##INFO= -##INFO= -##INFO= -##INFO= -##INFO= -##FILTER= -##FILTER= -##FILTER= -##FILTER= -##FILTER= 0.001000"> -##SnpEffVersion="4.5covid19 (build 2020-04-15 22:26), by Pablo Cingolani" -##SnpEffCmd="SnpEff -i vcf -o vcf -formatEff -classic -no-downstream -no-intergenic -no-upstream -no-utr -stats /Users/mvandenb/src/galaxy/database/files/f/0/0/dataset_f0009ccd-1b5f-4f33-9a1b-ae6776cee9aa.dat NC_045512.2 /Users/mvandenb/src/galaxy/database/files/f/d/1/dataset_fd11f62e-8834-447e-bf44-9edb96ef3c18.dat " -##INFO= -##INFO= -##INFO= -#CHROM POS ID REF ALT QUAL FILTER INFO -NC_045512.2 90 . G T 147.0 PASS DP=54;AF=0.111111;SB=4;DP4=19,29,1,5 -NC_045512.2 100 . C T 87.0 PASS DP=64;AF=0.062500;SB=5;DP4=22,38,3,1 -NC_045512.2 185 . G T 138.0 PASS DP=90;AF=0.077778;SB=1;DP4=39,44,4,3 -NC_045512.2 241 . C T 2847.0 PASS DP=81;AF=1.000000;SB=0;DP4=0,0,38,43 -NC_045512.2 419 . G T 105.0 PASS DP=93;AF=0.064516;SB=0;DP4=49,38,3,3;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Ggc/Tgc|G52C|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Ggc/Tgc|G52C|179|ORF1ab|protein_coding|CODING|YP_009725297.1|1|T|WARNING_TRANSCRIPT_NO_STOP_CODON),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Ggc/Tgc|G52C|179|ORF1ab|protein_coding|CODING|YP_009742608.1|1|T|WARNING_TRANSCRIPT_NO_STOP_CODON),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Ggc/Tgc|G52C|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|T) -NC_045512.2 675 . GC G 65.0 PASS DP=79;AF=0.037975;SB=0;DP4=49,28,2,1;INDEL;HRUN=1;EFF=FRAME_SHIFT(HIGH||ggc/|G137|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|G),FRAME_SHIFT(HIGH||ggc/|G137|179|ORF1ab|protein_coding|CODING|YP_009725297.1|1|G|WARNING_TRANSCRIPT_NO_STOP_CODON),FRAME_SHIFT(HIGH||ggc/|G137|179|ORF1ab|protein_coding|CODING|YP_009742608.1|1|G|WARNING_TRANSCRIPT_NO_STOP_CODON),FRAME_SHIFT(HIGH||ggc/|G137|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|G);LOF=(ORF1ab|GU280_gp01|28|0.07) -NC_045512.2 684 . TA T 66.0 PASS DP=80;AF=0.037500;SB=0;DP4=47,30,2,1;INDEL;HRUN=3;EFF=FRAME_SHIFT(HIGH||aag/|K141|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|T|INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||aag/|K141|179|ORF1ab|protein_coding|CODING|YP_009725297.1|1|T|WARNING_TRANSCRIPT_NO_STOP_CODON&INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||aag/|K141|179|ORF1ab|protein_coding|CODING|YP_009742608.1|1|T|WARNING_TRANSCRIPT_NO_STOP_CODON&INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||aag/|K141|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|T|INFO_REALIGN_3_PRIME);LOF=(ORF1ab|GU280_gp01|28|0.07) -NC_045512.2 836 . TG T 68.0 PASS DP=92;AF=0.032609;SB=0;DP4=39,50,1,2;INDEL;HRUN=1;EFF=FRAME_SHIFT(HIGH||tgt/|C191|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|T),FRAME_SHIFT(HIGH||tgt/|C191|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|T),FRAME_SHIFT(HIGH||tgt/|C11|637|ORF1ab|protein_coding|CODING|YP_009725298.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON),FRAME_SHIFT(HIGH||tgt/|C11|637|ORF1ab|protein_coding|CODING|YP_009742609.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 866 . ATT A 94.0 PASS DP=90;AF=0.044444;SB=9;DP4=44,43,0,4;INDEL;HRUN=2;EFF=FRAME_SHIFT(HIGH||att/|I201|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|A),FRAME_SHIFT(HIGH||att/|I201|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|A),FRAME_SHIFT(HIGH||att/|I21|637|ORF1ab|protein_coding|CODING|YP_009725298.1|1|A|WARNING_TRANSCRIPT_NO_START_CODON),FRAME_SHIFT(HIGH||att/|I21|637|ORF1ab|protein_coding|CODING|YP_009742609.1|1|A|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 925 . C T 133.0 PASS DP=85;AF=0.070588;SB=3;DP4=37,42,4,2;EFF=SYNONYMOUS_CODING(LOW|SILENT|gaC/gaT|D220|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|T),SYNONYMOUS_CODING(LOW|SILENT|gaC/gaT|D220|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|T),SYNONYMOUS_CODING(LOW|SILENT|gaC/gaT|D40|637|ORF1ab|protein_coding|CODING|YP_009725298.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON),SYNONYMOUS_CODING(LOW|SILENT|gaC/gaT|D40|637|ORF1ab|protein_coding|CODING|YP_009742609.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 974 . G A 83.0 PASS DP=86;AF=0.046512;SB=2;DP4=44,38,3,1;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gaa/Aaa|E237K|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|A),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gaa/Aaa|E237K|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|A),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gaa/Aaa|E57K|637|ORF1ab|protein_coding|CODING|YP_009725298.1|1|A|WARNING_TRANSCRIPT_NO_START_CODON),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gaa/Aaa|E57K|637|ORF1ab|protein_coding|CODING|YP_009742609.1|1|A|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 1191 . CA C 61.0 PASS DP=92;AF=0.032609;SB=2;DP4=38,51,2,1;INDEL;HRUN=3;EFF=FRAME_SHIFT(HIGH||aat/|N310|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|C|INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||aat/|N310|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|C|INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||aat/|N130|637|ORF1ab|protein_coding|CODING|YP_009725298.1|1|C|WARNING_TRANSCRIPT_NO_START_CODON&INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||aat/|N130|637|ORF1ab|protein_coding|CODING|YP_009742609.1|1|C|WARNING_TRANSCRIPT_NO_START_CODON&INFO_REALIGN_3_PRIME);LOF=(ORF1ab|GU280_gp01|28|0.11) -NC_045512.2 1258 . G T 127.0 PASS DP=106;AF=0.056604;SB=0;DP4=51,49,3,3;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|tgG/tgT|W331C|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|tgG/tgT|W331C|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|tgG/tgT|W151C|637|ORF1ab|protein_coding|CODING|YP_009725298.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|tgG/tgT|W151C|637|ORF1ab|protein_coding|CODING|YP_009742609.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 1720 . TG T 63.0 PASS DP=106;AF=0.028302;SB=0;DP4=51,52,2,1;INDEL;HRUN=1;EFF=FRAME_SHIFT(HIGH||gct/|A486|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|T),FRAME_SHIFT(HIGH||gct/|A486|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|T),FRAME_SHIFT(HIGH||gct/|A306|637|ORF1ab|protein_coding|CODING|YP_009725298.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON),FRAME_SHIFT(HIGH||gct/|A306|637|ORF1ab|protein_coding|CODING|YP_009742609.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON);LOF=(ORF1ab|GU280_gp01|28|0.14) -NC_045512.2 1752 . AT A 80.0 PASS DP=107;AF=0.037383;SB=9;DP4=52,52,4,0;INDEL;HRUN=1;EFF=FRAME_SHIFT(HIGH||tat/|Y496|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|A),FRAME_SHIFT(HIGH||tat/|Y496|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|A),FRAME_SHIFT(HIGH||tat/|Y316|637|ORF1ab|protein_coding|CODING|YP_009725298.1|1|A|WARNING_TRANSCRIPT_NO_START_CODON),FRAME_SHIFT(HIGH||tat/|Y316|637|ORF1ab|protein_coding|CODING|YP_009742609.1|1|A|WARNING_TRANSCRIPT_NO_START_CODON);LOF=(ORF1ab|GU280_gp01|28|0.14) -NC_045512.2 1931 . CA C 67.0 PASS DP=91;AF=0.032967;SB=2;DP4=49,40,1,2;INDEL;HRUN=4;EFF=FRAME_SHIFT(HIGH||aat/|N557|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|C|INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||aat/|N557|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|C|INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||aat/|N377|637|ORF1ab|protein_coding|CODING|YP_009725298.1|1|C|WARNING_TRANSCRIPT_NO_START_CODON&INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||aat/|N377|637|ORF1ab|protein_coding|CODING|YP_009742609.1|1|C|WARNING_TRANSCRIPT_NO_START_CODON&INFO_REALIGN_3_PRIME);LOF=(ORF1ab|GU280_gp01|28|0.14) -NC_045512.2 2268 . CA C 63.0 PASS DP=70;AF=0.042857;SB=11;DP4=40,28,0,3;INDEL;HRUN=3;EFF=FRAME_SHIFT(HIGH||aag/|K669|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|C|INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||aag/|K669|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|C|INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||aag/|K489|637|ORF1ab|protein_coding|CODING|YP_009725298.1|1|C|WARNING_TRANSCRIPT_NO_START_CODON&INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||aag/|K489|637|ORF1ab|protein_coding|CODING|YP_009742609.1|1|C|WARNING_TRANSCRIPT_NO_START_CODON&INFO_REALIGN_3_PRIME);LOF=(ORF1ab|GU280_gp01|28|0.14) -NC_045512.2 2381 . G T 114.0 PASS DP=66;AF=0.075758;SB=4;DP4=30,31,4,1;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Ggt/Tgt|G706C|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Ggt/Tgt|G706C|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Ggt/Tgt|G526C|637|ORF1ab|protein_coding|CODING|YP_009725298.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Ggt/Tgt|G526C|637|ORF1ab|protein_coding|CODING|YP_009742609.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 2416 . C T 2300.0 PASS DP=65;AF=1.000000;SB=0;DP4=0,0,34,31;EFF=SYNONYMOUS_CODING(LOW|SILENT|taC/taT|Y717|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|T),SYNONYMOUS_CODING(LOW|SILENT|taC/taT|Y717|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|T),SYNONYMOUS_CODING(LOW|SILENT|taC/taT|Y537|637|ORF1ab|protein_coding|CODING|YP_009725298.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON),SYNONYMOUS_CODING(LOW|SILENT|taC/taT|Y537|637|ORF1ab|protein_coding|CODING|YP_009742609.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 2463 . TA T 128.0 PASS DP=63;AF=0.079365;SB=1;DP4=25,33,3,2;INDEL;HRUN=4;EFF=FRAME_SHIFT(HIGH||aaa/|K734|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|T|INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||aaa/|K734|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|T|INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||aaa/|K554|637|ORF1ab|protein_coding|CODING|YP_009725298.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON&INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||aaa/|K554|637|ORF1ab|protein_coding|CODING|YP_009742609.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON&INFO_REALIGN_3_PRIME);LOF=(ORF1ab|GU280_gp01|28|0.14) -NC_045512.2 2523 . C T 140.0 PASS DP=66;AF=0.090909;SB=1;DP4=30,30,2,4;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aCa/aTa|T753I|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aCa/aTa|T753I|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aCa/aTa|T573I|637|ORF1ab|protein_coding|CODING|YP_009725298.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aCa/aTa|T573I|637|ORF1ab|protein_coding|CODING|YP_009742609.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 2695 . C T 75.0 PASS DP=25;AF=0.120000;SB=2;DP4=10,12,2,1;EFF=SYNONYMOUS_CODING(LOW|SILENT|aaC/aaT|N810|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|T),SYNONYMOUS_CODING(LOW|SILENT|aaC/aaT|N810|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|T),SYNONYMOUS_CODING(LOW|SILENT|aaC/aaT|N630|637|ORF1ab|protein_coding|CODING|YP_009725298.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON),SYNONYMOUS_CODING(LOW|SILENT|aaC/aaT|N630|637|ORF1ab|protein_coding|CODING|YP_009742609.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 3037 . C T 2892.0 PASS DP=81;AF=1.000000;SB=0;DP4=0,0,40,41;EFF=SYNONYMOUS_CODING(LOW|SILENT|ttC/ttT|F924|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|T),SYNONYMOUS_CODING(LOW|SILENT|ttC/ttT|F924|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|T),SYNONYMOUS_CODING(LOW|SILENT|ttC/ttT|F106|1944|ORF1ab|protein_coding|CODING|YP_009725299.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON),SYNONYMOUS_CODING(LOW|SILENT|ttC/ttT|F106|1944|ORF1ab|protein_coding|CODING|YP_009742610.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 3251 . G T 68.0 PASS DP=108;AF=0.037037;SB=0;DP4=55,49,2,2;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gac/Tac|D996Y|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gac/Tac|D996Y|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gac/Tac|D178Y|1944|ORF1ab|protein_coding|CODING|YP_009725299.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gac/Tac|D178Y|1944|ORF1ab|protein_coding|CODING|YP_009742610.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 3259 . G T 80.0 PASS DP=104;AF=0.038462;SB=4;DP4=54,46,1,3;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|caG/caT|Q998H|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|caG/caT|Q998H|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|caG/caT|Q180H|1944|ORF1ab|protein_coding|CODING|YP_009725299.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|caG/caT|Q180H|1944|ORF1ab|protein_coding|CODING|YP_009742610.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 3464 . C T 137.0 PASS DP=76;AF=0.078947;SB=6;DP4=33,37,1,5;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Cat/Tat|H1067Y|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Cat/Tat|H1067Y|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Cat/Tat|H249Y|1944|ORF1ab|protein_coding|CODING|YP_009725299.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Cat/Tat|H249Y|1944|ORF1ab|protein_coding|CODING|YP_009742610.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 3540 . C T 89.0 PASS DP=59;AF=0.067797;SB=0;DP4=30,25,2,2;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gCt/gTt|A1092V|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gCt/gTt|A1092V|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gCt/gTt|A274V|1944|ORF1ab|protein_coding|CODING|YP_009725299.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gCt/gTt|A274V|1944|ORF1ab|protein_coding|CODING|YP_009742610.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 3751 . AAG A 129.0 PASS DP=64;AF=0.078125;SB=1;DP4=22,37,1,4;INDEL;HRUN=2;EFF=FRAME_SHIFT(HIGH||agagtt/|RV1163|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|A|INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||agagtt/|RV1163|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|A|INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||agagtt/|RV345|1944|ORF1ab|protein_coding|CODING|YP_009725299.1|1|A|WARNING_TRANSCRIPT_NO_START_CODON&INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||agagtt/|RV345|1944|ORF1ab|protein_coding|CODING|YP_009742610.1|1|A|WARNING_TRANSCRIPT_NO_START_CODON&INFO_REALIGN_3_PRIME);LOF=(ORF1ab|GU280_gp01|28|0.14) -NC_045512.2 3923 . C T 370.0 PASS DP=82;AF=0.182927;SB=1;DP4=35,32,9,6;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Cct/Tct|P1220S|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Cct/Tct|P1220S|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Cct/Tct|P402S|1944|ORF1ab|protein_coding|CODING|YP_009725299.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Cct/Tct|P402S|1944|ORF1ab|protein_coding|CODING|YP_009742610.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 4281 . TA T 97.0 PASS DP=72;AF=0.055556;SB=2;DP4=36,33,3,1;INDEL;HRUN=1;EFF=FRAME_SHIFT(HIGH||gta/|V1339|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|T),FRAME_SHIFT(HIGH||gta/|V1339|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|T),FRAME_SHIFT(HIGH||gta/|V521|1944|ORF1ab|protein_coding|CODING|YP_009725299.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON),FRAME_SHIFT(HIGH||gta/|V521|1944|ORF1ab|protein_coding|CODING|YP_009742610.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON);LOF=(ORF1ab|GU280_gp01|28|0.14) -NC_045512.2 4312 . TA T 96.0 PASS DP=76;AF=0.052632;SB=9;DP4=36,36,4,0;INDEL;HRUN=4;EFF=FRAME_SHIFT(HIGH||agt/|S1351|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|T|INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||agt/|S1351|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|T|INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||agt/|S533|1944|ORF1ab|protein_coding|CODING|YP_009725299.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON&INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||agt/|S533|1944|ORF1ab|protein_coding|CODING|YP_009742610.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON&INFO_REALIGN_3_PRIME);LOF=(ORF1ab|GU280_gp01|28|0.14) -NC_045512.2 4505 . G T 98.0 PASS DP=81;AF=0.061728;SB=4;DP4=39,37,4,1;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Ggt/Tgt|G1414C|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Ggt/Tgt|G1414C|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Ggt/Tgt|G596C|1944|ORF1ab|protein_coding|CODING|YP_009725299.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Ggt/Tgt|G596C|1944|ORF1ab|protein_coding|CODING|YP_009742610.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 4523 . G A 2863.0 PASS DP=80;AF=1.000000;SB=0;DP4=0,0,43,37;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gct/Act|A1420T|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|A),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gct/Act|A1420T|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|A),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gct/Act|A602T|1944|ORF1ab|protein_coding|CODING|YP_009725299.1|1|A|WARNING_TRANSCRIPT_NO_START_CODON),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gct/Act|A602T|1944|ORF1ab|protein_coding|CODING|YP_009742610.1|1|A|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 5301 . CA C 74.0 PASS DP=53;AF=0.056604;SB=2;DP4=28,23,1,2;INDEL;HRUN=1;EFF=FRAME_SHIFT(HIGH||gca/|A1679|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|C),FRAME_SHIFT(HIGH||gca/|A1679|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|C),FRAME_SHIFT(HIGH||gca/|A861|1944|ORF1ab|protein_coding|CODING|YP_009725299.1|1|C|WARNING_TRANSCRIPT_NO_START_CODON),FRAME_SHIFT(HIGH||gca/|A861|1944|ORF1ab|protein_coding|CODING|YP_009742610.1|1|C|WARNING_TRANSCRIPT_NO_START_CODON);LOF=(ORF1ab|GU280_gp01|28|0.14) -NC_045512.2 5575 . CA C 70.0 PASS DP=72;AF=0.041667;SB=2;DP4=29,42,2,1;INDEL;HRUN=1;EFF=FRAME_SHIFT(HIGH||atg/|M1771|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|C),FRAME_SHIFT(HIGH||atg/|M1771|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|C),FRAME_SHIFT(HIGH||atg/|M953|1944|ORF1ab|protein_coding|CODING|YP_009725299.1|1|C|WARNING_TRANSCRIPT_NO_START_CODON),FRAME_SHIFT(HIGH||atg/|M953|1944|ORF1ab|protein_coding|CODING|YP_009742610.1|1|C|WARNING_TRANSCRIPT_NO_START_CODON);LOF=(ORF1ab|GU280_gp01|28|0.14) -NC_045512.2 5597 . C T 84.0 PASS DP=85;AF=0.047059;SB=2;DP4=40,41,1,3;EFF=STOP_GAINED(HIGH|NONSENSE|Caa/Taa|Q1778*|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|T),STOP_GAINED(HIGH|NONSENSE|Caa/Taa|Q1778*|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|T),STOP_GAINED(HIGH|NONSENSE|Caa/Taa|Q960*|1944|ORF1ab|protein_coding|CODING|YP_009725299.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON),STOP_GAINED(HIGH|NONSENSE|Caa/Taa|Q960*|1944|ORF1ab|protein_coding|CODING|YP_009742610.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON);LOF=(ORF1ab|GU280_gp01|28|0.04);NMD=(ORF1ab|GU280_gp01|28|0.04) -NC_045512.2 5632 . TG T 78.0 PASS DP=84;AF=0.047619;SB=4;DP4=38,43,3,1;INDEL;HRUN=2;EFF=FRAME_SHIFT(HIGH||ggt/|G1790|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|T|INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||ggt/|G1790|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|T|INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||ggt/|G972|1944|ORF1ab|protein_coding|CODING|YP_009725299.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON&INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||ggt/|G972|1944|ORF1ab|protein_coding|CODING|YP_009742610.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON&INFO_REALIGN_3_PRIME);LOF=(ORF1ab|GU280_gp01|28|0.14) -NC_045512.2 5757 . A T 108.0 PASS DP=81;AF=0.061728;SB=0;DP4=38,38,3,2;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|tAc/tTc|Y1831F|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|tAc/tTc|Y1831F|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|tAc/tTc|Y1013F|1944|ORF1ab|protein_coding|CODING|YP_009725299.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|tAc/tTc|Y1013F|1944|ORF1ab|protein_coding|CODING|YP_009742610.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 5810 . G T 106.0 PASS DP=81;AF=0.061728;SB=1;DP4=36,40,3,2;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gac/Tac|D1849Y|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gac/Tac|D1849Y|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gac/Tac|D1031Y|1944|ORF1ab|protein_coding|CODING|YP_009725299.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gac/Tac|D1031Y|1944|ORF1ab|protein_coding|CODING|YP_009742610.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 5813 . G T 131.0 PASS DP=80;AF=0.075000;SB=3;DP4=34,40,4,2;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Ggt/Tgt|G1850C|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Ggt/Tgt|G1850C|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Ggt/Tgt|G1032C|1944|ORF1ab|protein_coding|CODING|YP_009725299.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Ggt/Tgt|G1032C|1944|ORF1ab|protein_coding|CODING|YP_009742610.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 5945 . C T 211.0 PASS DP=88;AF=0.102273;SB=0;DP4=37,41,4,5;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Cct/Tct|P1894S|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Cct/Tct|P1894S|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Cct/Tct|P1076S|1944|ORF1ab|protein_coding|CODING|YP_009725299.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Cct/Tct|P1076S|1944|ORF1ab|protein_coding|CODING|YP_009742610.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 6021 . C T 82.0 PASS DP=80;AF=0.050000;SB=0;DP4=44,32,2,2;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cCa/cTa|P1919L|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cCa/cTa|P1919L|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cCa/cTa|P1101L|1944|ORF1ab|protein_coding|CODING|YP_009725299.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cCa/cTa|P1101L|1944|ORF1ab|protein_coding|CODING|YP_009742610.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 6171 . C T 80.0 PASS DP=85;AF=0.047059;SB=2;DP4=30,51,2,2;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gCt/gTt|A1969V|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gCt/gTt|A1969V|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gCt/gTt|A1151V|1944|ORF1ab|protein_coding|CODING|YP_009725299.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gCt/gTt|A1151V|1944|ORF1ab|protein_coding|CODING|YP_009742610.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 6310 . CA C 149.0 PASS DP=90;AF=0.066667;SB=0;DP4=46,39,3,3;INDEL;HRUN=1;EFF=FRAME_SHIFT(HIGH||aca/|T2016|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|C),FRAME_SHIFT(HIGH||aca/|T2016|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|C),FRAME_SHIFT(HIGH||aca/|T1198|1944|ORF1ab|protein_coding|CODING|YP_009725299.1|1|C|WARNING_TRANSCRIPT_NO_START_CODON),FRAME_SHIFT(HIGH||aca/|T1198|1944|ORF1ab|protein_coding|CODING|YP_009742610.1|1|C|WARNING_TRANSCRIPT_NO_START_CODON);LOF=(ORF1ab|GU280_gp01|28|0.14) -NC_045512.2 6312 . C T 104.0 PASS DP=90;AF=0.055556;SB=0;DP4=46,39,3,2;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aCa/aTa|T2016I|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aCa/aTa|T2016I|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aCa/aTa|T1198I|1944|ORF1ab|protein_coding|CODING|YP_009725299.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aCa/aTa|T1198I|1944|ORF1ab|protein_coding|CODING|YP_009742610.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 6513 . G GT 69.0 PASS DP=82;AF=0.036585;SB=2;DP4=44,37,1,2;INDEL;HRUN=3;EFF=FRAME_SHIFT(HIGH||tta/ttTa|L2084F?|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|GT|INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||tta/ttTa|L2084F?|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|GT|INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||tta/ttTa|L1266F?|1944|ORF1ab|protein_coding|CODING|YP_009725299.1|1|GT|WARNING_TRANSCRIPT_NO_START_CODON&INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||tta/ttTa|L1266F?|1944|ORF1ab|protein_coding|CODING|YP_009742610.1|1|GT|WARNING_TRANSCRIPT_NO_START_CODON&INFO_REALIGN_3_PRIME);LOF=(ORF1ab|GU280_gp01|28|0.14) -NC_045512.2 6696 . C CT 356.0 PASS DP=81;AF=0.160494;SB=0;DP4=35,34,7,6;INDEL;HRUN=4;EFF=FRAME_SHIFT(HIGH||ctt/Tctt|L2146S?|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|CT|INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||ctt/Tctt|L2146S?|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|CT|INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||ctt/Tctt|L1328S?|1944|ORF1ab|protein_coding|CODING|YP_009725299.1|1|CT|WARNING_TRANSCRIPT_NO_START_CODON&INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||ctt/Tctt|L1328S?|1944|ORF1ab|protein_coding|CODING|YP_009742610.1|1|CT|WARNING_TRANSCRIPT_NO_START_CODON&INFO_REALIGN_3_PRIME);LOF=(ORF1ab|GU280_gp01|28|0.14) -NC_045512.2 6696 . C T 84.0 PASS DP=82;AF=0.048780;SB=0;DP4=40,38,2,2;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cCt/cTt|P2144L|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cCt/cTt|P2144L|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cCt/cTt|P1326L|1944|ORF1ab|protein_coding|CODING|YP_009725299.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cCt/cTt|P1326L|1944|ORF1ab|protein_coding|CODING|YP_009742610.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 6736 . TAC T 67.0 PASS DP=94;AF=0.031915;SB=6;DP4=47,46,3,0;INDEL;HRUN=1;EFF=FRAME_SHIFT(HIGH||acacgg/|TR2158|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|T|INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||acacgg/|TR2158|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|T|INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||acacgg/|TR1340|1944|ORF1ab|protein_coding|CODING|YP_009725299.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON&INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||acacgg/|TR1340|1944|ORF1ab|protein_coding|CODING|YP_009742610.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON&INFO_REALIGN_3_PRIME);LOF=(ORF1ab|GU280_gp01|28|0.14) -NC_045512.2 6961 . GTT G 92.0 PASS DP=83;AF=0.048193;SB=2;DP4=40,41,1,3;INDEL;HRUN=5;EFF=FRAME_SHIFT(HIGH||tta/|L2234|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|G|INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||tta/|L2234|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|G|INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||tta/|L1416|1944|ORF1ab|protein_coding|CODING|YP_009725299.1|1|G|WARNING_TRANSCRIPT_NO_START_CODON&INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||tta/|L1416|1944|ORF1ab|protein_coding|CODING|YP_009742610.1|1|G|WARNING_TRANSCRIPT_NO_START_CODON&INFO_REALIGN_3_PRIME);LOF=(ORF1ab|GU280_gp01|28|0.14) -NC_045512.2 7230 . G T 68.0 PASS DP=54;AF=0.074074;SB=5;DP4=20,30,3,1;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gGc/gTc|G2322V|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gGc/gTc|G2322V|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gGc/gTc|G1504V|1944|ORF1ab|protein_coding|CODING|YP_009725299.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gGc/gTc|G1504V|1944|ORF1ab|protein_coding|CODING|YP_009742610.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 7307 . CA C 74.0 PASS DP=57;AF=0.052632;SB=2;DP4=26,28,2,1;INDEL;HRUN=2;EFF=FRAME_SHIFT(HIGH||caa/|Q2348|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|C|INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||caa/|Q2348|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|C|INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||caa/|Q1530|1944|ORF1ab|protein_coding|CODING|YP_009725299.1|1|C|WARNING_TRANSCRIPT_NO_START_CODON&INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||caa/|Q1530|1944|ORF1ab|protein_coding|CODING|YP_009742610.1|1|C|WARNING_TRANSCRIPT_NO_START_CODON&INFO_REALIGN_3_PRIME);LOF=(ORF1ab|GU280_gp01|28|0.14) -NC_045512.2 7491 . G C 81.0 PASS DP=94;AF=0.042553;SB=4;DP4=43,47,3,1;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|tGt/tCt|C2409S|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|C),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|tGt/tCt|C2409S|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|C),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|tGt/tCt|C1591S|1944|ORF1ab|protein_coding|CODING|YP_009725299.1|1|C|WARNING_TRANSCRIPT_NO_START_CODON),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|tGt/tCt|C1591S|1944|ORF1ab|protein_coding|CODING|YP_009742610.1|1|C|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 7579 . T C 84.0 PASS DP=86;AF=0.046512;SB=9;DP4=43,39,0,4;EFF=SYNONYMOUS_CODING(LOW|SILENT|gcT/gcC|A2438|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|C),SYNONYMOUS_CODING(LOW|SILENT|gcT/gcC|A2438|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|C),SYNONYMOUS_CODING(LOW|SILENT|gcT/gcC|A1620|1944|ORF1ab|protein_coding|CODING|YP_009725299.1|1|C|WARNING_TRANSCRIPT_NO_START_CODON),SYNONYMOUS_CODING(LOW|SILENT|gcT/gcC|A1620|1944|ORF1ab|protein_coding|CODING|YP_009742610.1|1|C|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 7772 . CT C 68.0 PASS DP=93;AF=0.032258;SB=2;DP4=47,44,1,2;INDEL;HRUN=3;EFF=FRAME_SHIFT(HIGH||tac/|Y2504|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|C|INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||tac/|Y2504|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|C|INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||tac/|Y1686|1944|ORF1ab|protein_coding|CODING|YP_009725299.1|1|C|WARNING_TRANSCRIPT_NO_START_CODON&INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||tac/|Y1686|1944|ORF1ab|protein_coding|CODING|YP_009742610.1|1|C|WARNING_TRANSCRIPT_NO_START_CODON&INFO_REALIGN_3_PRIME);LOF=(ORF1ab|GU280_gp01|28|0.14) -NC_045512.2 8011 . TA T 124.0 PASS DP=74;AF=0.067568;SB=0;DP4=39,36,3,2;INDEL;HRUN=1;EFF=FRAME_SHIFT(HIGH||agt/|S2583|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|T),FRAME_SHIFT(HIGH||agt/|S2583|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|T),FRAME_SHIFT(HIGH||agt/|S1765|1944|ORF1ab|protein_coding|CODING|YP_009725299.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON),FRAME_SHIFT(HIGH||agt/|S1765|1944|ORF1ab|protein_coding|CODING|YP_009742610.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON);LOF=(ORF1ab|GU280_gp01|28|0.14) -NC_045512.2 8306 . GA G 70.0 PASS DP=70;AF=0.042857;SB=0;DP4=37,31,2,1;INDEL;HRUN=4;EFF=FRAME_SHIFT(HIGH||aac/|N2682|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|G|INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||aac/|N2682|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|G|INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||aac/|N1864|1944|ORF1ab|protein_coding|CODING|YP_009725299.1|1|G|WARNING_TRANSCRIPT_NO_START_CODON&INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||aac/|N1864|1944|ORF1ab|protein_coding|CODING|YP_009742610.1|1|G|WARNING_TRANSCRIPT_NO_START_CODON&INFO_REALIGN_3_PRIME);LOF=(ORF1ab|GU280_gp01|28|0.07) -NC_045512.2 8457 . G T 84.0 PASS DP=80;AF=0.050000;SB=0;DP4=34,42,2,2;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aGt/aTt|S2731I|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aGt/aTt|S2731I|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aGt/aTt|S1913I|1944|ORF1ab|protein_coding|CODING|YP_009725299.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aGt/aTt|S1913I|1944|ORF1ab|protein_coding|CODING|YP_009742610.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 8520 . A T 133.0 PASS DP=82;AF=0.073171;SB=6;DP4=37,39,1,5;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aAt/aTt|N2752I|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aAt/aTt|N2752I|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aAt/aTt|N1934I|1944|ORF1ab|protein_coding|CODING|YP_009725299.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aAt/aTt|N1934I|1944|ORF1ab|protein_coding|CODING|YP_009742610.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 8748 . C T 105.0 PASS DP=102;AF=0.049020;SB=4;DP4=52,45,4,1;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gCt/gTt|A2828V|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gCt/gTt|A2828V|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gCt/gTt|A65V|499|ORF1ab|protein_coding|CODING|YP_009725300.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gCt/gTt|A65V|499|ORF1ab|protein_coding|CODING|YP_009742611.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 8802 . C T 105.0 PASS DP=97;AF=0.051546;SB=1;DP4=43,49,3,2;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aCt/aTt|T2846I|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aCt/aTt|T2846I|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aCt/aTt|T83I|499|ORF1ab|protein_coding|CODING|YP_009725300.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aCt/aTt|T83I|499|ORF1ab|protein_coding|CODING|YP_009742611.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 8872 . G C 153.0 PASS DP=91;AF=0.076923;SB=5;DP4=40,44,5,2;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|ttG/ttC|L2869F|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|C),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|ttG/ttC|L2869F|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|C),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|ttG/ttC|L106F|499|ORF1ab|protein_coding|CODING|YP_009725300.1|1|C|WARNING_TRANSCRIPT_NO_START_CODON),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|ttG/ttC|L106F|499|ORF1ab|protein_coding|CODING|YP_009742611.1|1|C|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 9205 . TTC T 129.0 PASS DP=64;AF=0.078125;SB=0;DP4=30,30,2,3;INDEL;HRUN=2;EFF=FRAME_SHIFT(HIGH||tct/|S2981|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|T|INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||tct/|S2981|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|T|INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||tct/|S218|499|ORF1ab|protein_coding|CODING|YP_009725300.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON&INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||tct/|S218|499|ORF1ab|protein_coding|CODING|YP_009742611.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON&INFO_REALIGN_3_PRIME);LOF=(ORF1ab|GU280_gp01|28|0.14) -NC_045512.2 9355 . G GT 68.0 PASS DP=88;AF=0.034091;SB=2;DP4=41,45,2,1;INDEL;HRUN=3;EFF=FRAME_SHIFT(HIGH||aca/Taca|T3032Y?|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|GT|INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||aca/Taca|T3032Y?|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|GT|INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||aca/Taca|T269Y?|499|ORF1ab|protein_coding|CODING|YP_009725300.1|1|GT|WARNING_TRANSCRIPT_NO_START_CODON&INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||aca/Taca|T269Y?|499|ORF1ab|protein_coding|CODING|YP_009742611.1|1|GT|WARNING_TRANSCRIPT_NO_START_CODON&INFO_REALIGN_3_PRIME);LOF=(ORF1ab|GU280_gp01|28|0.14) -NC_045512.2 9430 . C T 103.0 PASS DP=93;AF=0.053763;SB=0;DP4=44,44,2,3;EFF=SYNONYMOUS_CODING(LOW|SILENT|atC/atT|I3055|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|T),SYNONYMOUS_CODING(LOW|SILENT|atC/atT|I3055|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|T),SYNONYMOUS_CODING(LOW|SILENT|atC/atT|I292|499|ORF1ab|protein_coding|CODING|YP_009725300.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON),SYNONYMOUS_CODING(LOW|SILENT|atC/atT|I292|499|ORF1ab|protein_coding|CODING|YP_009742611.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 9461 . AG A 68.0 PASS DP=89;AF=0.033708;SB=2;DP4=43,45,2,1;INDEL;HRUN=2;EFF=FRAME_SHIFT(HIGH||agg/|R3066|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|A|INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||agg/|R3066|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|A|INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||agg/|R303|499|ORF1ab|protein_coding|CODING|YP_009725300.1|1|A|WARNING_TRANSCRIPT_NO_START_CODON&INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||agg/|R303|499|ORF1ab|protein_coding|CODING|YP_009742611.1|1|A|WARNING_TRANSCRIPT_NO_START_CODON&INFO_REALIGN_3_PRIME);LOF=(ORF1ab|GU280_gp01|28|0.14) -NC_045512.2 9747 . TA T 102.0 PASS DP=108;AF=0.046296;SB=6;DP4=50,54,4,1;INDEL;HRUN=3;EFF=FRAME_SHIFT(HIGH||aag/|K3162|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|T|INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||aag/|K3162|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|T|INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||aag/|K399|499|ORF1ab|protein_coding|CODING|YP_009725300.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON&INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||aag/|K399|499|ORF1ab|protein_coding|CODING|YP_009742611.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON&INFO_REALIGN_3_PRIME);LOF=(ORF1ab|GU280_gp01|28|0.14) -NC_045512.2 9851 . GATGTGCTA G 58.0 PASS DP=77;AF=0.038961;SB=5;DP4=42,33,3,0;INDEL;HRUN=1;EFF=FRAME_SHIFT(HIGH||gatgtgcta/|DVL3196|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|G),FRAME_SHIFT(HIGH||gatgtgcta/|DVL3196|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|G),FRAME_SHIFT(HIGH||gatgtgcta/|DVL433|499|ORF1ab|protein_coding|CODING|YP_009725300.1|1|G|WARNING_TRANSCRIPT_NO_START_CODON),FRAME_SHIFT(HIGH||gatgtgcta/|DVL433|499|ORF1ab|protein_coding|CODING|YP_009742611.1|1|G|WARNING_TRANSCRIPT_NO_START_CODON);LOF=(ORF1ab|GU280_gp01|28|0.14) -NC_045512.2 10059 . G T 100.0 PASS DP=107;AF=0.046729;SB=1;DP4=49,53,3,2;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gGt/gTt|G3265V|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gGt/gTt|G3265V|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gGt/gTt|G2V|305|ORF1ab|protein_coding|CODING|YP_009725301.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gGt/gTt|G2V|305|ORF1ab|protein_coding|CODING|YP_009742612.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 10252 . CT C 273.0 PASS DP=75;AF=0.133333;SB=0;DP4=34,32,5,5;INDEL;HRUN=2;EFF=FRAME_SHIFT(HIGH||ttg/|L3330|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|C|INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||ttg/|L3330|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|C|INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||ttg/|L67|305|ORF1ab|protein_coding|CODING|YP_009725301.1|1|C|WARNING_TRANSCRIPT_NO_START_CODON&INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||ttg/|L67|305|ORF1ab|protein_coding|CODING|YP_009742612.1|1|C|WARNING_TRANSCRIPT_NO_START_CODON&INFO_REALIGN_3_PRIME);LOF=(ORF1ab|GU280_gp01|28|0.14) -NC_045512.2 10529 . T C 156.0 PASS DP=91;AF=0.076923;SB=3;DP4=42,42,2,5;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Ttt/Ctt|F3422L|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|C),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Ttt/Ctt|F3422L|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|C),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Ttt/Ctt|F159L|305|ORF1ab|protein_coding|CODING|YP_009725301.1|1|C|WARNING_TRANSCRIPT_NO_START_CODON),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Ttt/Ctt|F159L|305|ORF1ab|protein_coding|CODING|YP_009742612.1|1|C|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 10673 . TG T 99.0 PASS DP=66;AF=0.060606;SB=0;DP4=32,31,2,2;INDEL;HRUN=2;EFF=FRAME_SHIFT(HIGH||tgg/|W3470|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|T|INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||tgg/|W3470|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|T|INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||tgg/|W207|305|ORF1ab|protein_coding|CODING|YP_009725301.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON&INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||tgg/|W207|305|ORF1ab|protein_coding|CODING|YP_009742612.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON&INFO_REALIGN_3_PRIME);LOF=(ORF1ab|GU280_gp01|28|0.14) -NC_045512.2 10710 . TTC T 149.0 PASS DP=87;AF=0.068966;SB=0;DP4=42,40,3,3;INDEL;HRUN=3;EFF=FRAME_SHIFT(HIGH||ctc/|L3483|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|T|INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||ctc/|L3483|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|T|INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||ctc/|L220|305|ORF1ab|protein_coding|CODING|YP_009725301.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON&INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||ctc/|L220|305|ORF1ab|protein_coding|CODING|YP_009742612.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON&INFO_REALIGN_3_PRIME);LOF=(ORF1ab|GU280_gp01|28|0.14) -NC_045512.2 10712 . C T 2883.0 PASS DP=89;AF=0.932584;SB=0;DP4=0,0,42,41;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Ctc/Ttc|L3483F|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Ctc/Ttc|L3483F|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Ctc/Ttc|L220F|305|ORF1ab|protein_coding|CODING|YP_009725301.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Ctc/Ttc|L220F|305|ORF1ab|protein_coding|CODING|YP_009742612.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 10969 . C T 139.0 PASS DP=97;AF=0.082474;SB=8;DP4=39,50,6,2;EFF=SYNONYMOUS_CODING(LOW|SILENT|ttC/ttT|F3568|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|T),SYNONYMOUS_CODING(LOW|SILENT|ttC/ttT|F3568|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|T),SYNONYMOUS_CODING(LOW|SILENT|ttC/ttT|F305|305|ORF1ab|protein_coding|CODING|YP_009725301.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON),SYNONYMOUS_CODING(LOW|SILENT|ttC/ttT|F305|305|ORF1ab|protein_coding|CODING|YP_009742612.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 11022 . CA C 70.0 PASS DP=72;AF=0.041667;SB=9;DP4=33,37,3,0;INDEL;HRUN=2;EFF=FRAME_SHIFT(HIGH||att/|I3587|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|C|INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||att/|I3587|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|C|INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||att/|I18|289|ORF1ab|protein_coding|CODING|YP_009725302.1|1|C|WARNING_TRANSCRIPT_NO_START_CODON&INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||att/|I18|289|ORF1ab|protein_coding|CODING|YP_009742613.1|1|C|WARNING_TRANSCRIPT_NO_START_CODON&INFO_REALIGN_3_PRIME);LOF=(ORF1ab|GU280_gp01|28|0.14) -NC_045512.2 11231 . C T 182.0 PASS DP=92;AF=0.086957;SB=3;DP4=45,39,3,5;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Cct/Tct|P3656S|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Cct/Tct|P3656S|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Cct/Tct|P87S|289|ORF1ab|protein_coding|CODING|YP_009725302.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Cct/Tct|P87S|289|ORF1ab|protein_coding|CODING|YP_009742613.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 11361 . CTG C 94.0 PASS DP=93;AF=0.043011;SB=0;DP4=44,47,2,2;INDEL;HRUN=1;EFF=FRAME_SHIFT(HIGH||gtgtat/|VY3700|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|C|INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||gtgtat/|VY3700|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|C|INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||gtgtat/|VY131|289|ORF1ab|protein_coding|CODING|YP_009725302.1|1|C|WARNING_TRANSCRIPT_NO_START_CODON&INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||gtgtat/|VY131|289|ORF1ab|protein_coding|CODING|YP_009742613.1|1|C|WARNING_TRANSCRIPT_NO_START_CODON&INFO_REALIGN_3_PRIME);LOF=(ORF1ab|GU280_gp01|28|0.14) -NC_045512.2 11574 . T C 83.0 PASS DP=91;AF=0.043956;SB=2;DP4=41,45,1,3;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|tTc/tCc|F3770S|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|C),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|tTc/tCc|F3770S|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|C),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|tTc/tCc|F201S|289|ORF1ab|protein_coding|CODING|YP_009725302.1|1|C|WARNING_TRANSCRIPT_NO_START_CODON),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|tTc/tCc|F201S|289|ORF1ab|protein_coding|CODING|YP_009742613.1|1|C|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 11896 . A G 83.0 PASS DP=87;AF=0.045977;SB=2;DP4=42,41,1,3;EFF=SYNONYMOUS_CODING(LOW|SILENT|caA/caG|Q3877|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|G),SYNONYMOUS_CODING(LOW|SILENT|caA/caG|Q3877|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|G),SYNONYMOUS_CODING(LOW|SILENT|caA/caG|Q18|82|ORF1ab|protein_coding|CODING|YP_009725303.1|1|G|WARNING_TRANSCRIPT_NO_START_CODON),SYNONYMOUS_CODING(LOW|SILENT|caA/caG|Q18|82|ORF1ab|protein_coding|CODING|YP_009742614.1|1|G|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 12141 . C T 77.0 PASS DP=105;AF=0.038095;SB=2;DP4=51,50,1,3;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aCt/aTt|T3959I|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aCt/aTt|T3959I|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aCt/aTt|T17I|197|ORF1ab|protein_coding|CODING|YP_009725304.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aCt/aTt|T17I|197|ORF1ab|protein_coding|CODING|YP_009742615.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 12164 . G T 75.0 PASS DP=115;AF=0.034783;SB=4;DP4=60,51,1,3;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gct/Tct|A3967S|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gct/Tct|A3967S|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gct/Tct|A25S|197|ORF1ab|protein_coding|CODING|YP_009725304.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gct/Tct|A25S|197|ORF1ab|protein_coding|CODING|YP_009742615.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 12196 . TA T 60.0 PASS DP=111;AF=0.027027;SB=0;DP4=60,49,2,1;INDEL;HRUN=5;EFF=FRAME_SHIFT(HIGH||aag/|K3979|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|T|INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||aag/|K3979|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|T|INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||aag/|K37|197|ORF1ab|protein_coding|CODING|YP_009725304.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON&INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||aag/|K37|197|ORF1ab|protein_coding|CODING|YP_009742615.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON&INFO_REALIGN_3_PRIME);LOF=(ORF1ab|GU280_gp01|28|0.14) -NC_045512.2 12235 . AT A 67.0 PASS DP=99;AF=0.030303;SB=9;DP4=52,46,0,3;INDEL;HRUN=3;EFF=FRAME_SHIFT(HIGH||ttt/|F3991|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|A|INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||ttt/|F3991|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|A|INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||ttt/|F49|197|ORF1ab|protein_coding|CODING|YP_009725304.1|1|A|WARNING_TRANSCRIPT_NO_START_CODON&INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||ttt/|F49|197|ORF1ab|protein_coding|CODING|YP_009742615.1|1|A|WARNING_TRANSCRIPT_NO_START_CODON&INFO_REALIGN_3_PRIME);LOF=(ORF1ab|GU280_gp01|28|0.14) -NC_045512.2 13077 . CT C 58.0 PASS DP=102;AF=0.029412;SB=0;DP4=52,48,2,1;INDEL;HRUN=4;EFF=FRAME_SHIFT(HIGH||ttt/|F4272|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|C|INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||ttt/|F4272|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|C|INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||ttt/|F19|138|ORF1ab|protein_coding|CODING|YP_009725306.1|1|C|WARNING_TRANSCRIPT_NO_START_CODON&INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||ttt/|F19|138|ORF1ab|protein_coding|CODING|YP_009742617.1|1|C|WARNING_TRANSCRIPT_NO_START_CODON&INFO_REALIGN_3_PRIME);LOF=(ORF1ab|GU280_gp01|28|0.11) -NC_045512.2 13350 . G GT 124.0 PASS DP=74;AF=0.067568;SB=4;DP4=36,34,4,1;INDEL;HRUN=4;EFF=FRAME_SHIFT(HIGH||aca/Taca|T4364Y?|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|GT|INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||aca/Taca|T4364Y?|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|GT|INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||aca/Taca|T111Y?|138|ORF1ab|protein_coding|CODING|YP_009725306.1|1|GT|WARNING_TRANSCRIPT_NO_START_CODON&INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||aca/Taca|T111Y?|138|ORF1ab|protein_coding|CODING|YP_009742617.1|1|GT|WARNING_TRANSCRIPT_NO_START_CODON&INFO_REALIGN_3_PRIME);LOF=(ORF1ab|GU280_gp01|28|0.11) -NC_045512.2 13414 . TG T 108.0 PASS DP=39;AF=0.102564;SB=0;DP4=24,11,3,1;INDEL;HRUN=1;EFF=FRAME_SHIFT(HIGH||gat/|D4384|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|T),FRAME_SHIFT(HIGH||gat/|D4384|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|T),FRAME_SHIFT(HIGH||gat/|D131|138|ORF1ab|protein_coding|CODING|YP_009725306.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON),FRAME_SHIFT(HIGH||gat/|D131|138|ORF1ab|protein_coding|CODING|YP_009742617.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON);LOF=(ORF1ab|GU280_gp01|28|0.11) -NC_045512.2 13744 . T C 81.0 PASS DP=103;AF=0.038835;SB=4;DP4=55,44,1,3;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Ttt/Ctt|F4494L|7096|ORF1ab|protein_coding|CODING|GU280_gp01|2|C),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Ttt/Ctt|F102L|931|ORF1ab|protein_coding|CODING|YP_009725307.1|2|C|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 13761 . CG C 119.0 PASS DP=104;AF=0.048077;SB=0;DP4=49,51,2,3;INDEL;HRUN=2;EFF=FRAME_SHIFT(HIGH||ggt/|G4500|7096|ORF1ab|protein_coding|CODING|GU280_gp01|2|C|INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||ggt/|G108|931|ORF1ab|protein_coding|CODING|YP_009725307.1|2|C|WARNING_TRANSCRIPT_NO_START_CODON&INFO_REALIGN_3_PRIME);LOF=(ORF1ab|GU280_gp01|28|0.07) -NC_045512.2 13931 . A T 83.0 PASS DP=81;AF=0.049383;SB=4;DP4=43,34,1,3;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gAt/gTt|D4556V|7096|ORF1ab|protein_coding|CODING|GU280_gp01|2|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gAt/gTt|D164V|931|ORF1ab|protein_coding|CODING|YP_009725307.1|2|T|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 14017 . TG T 139.0 PASS DP=114;AF=0.052632;SB=1;DP4=54,54,4,2;INDEL;HRUN=1;EFF=FRAME_SHIFT(HIGH||tgt/|C4585|7096|ORF1ab|protein_coding|CODING|GU280_gp01|2|T),FRAME_SHIFT(HIGH||tgt/|C193|931|ORF1ab|protein_coding|CODING|YP_009725307.1|2|T|WARNING_TRANSCRIPT_NO_START_CODON);LOF=(ORF1ab|GU280_gp01|28|0.07) -NC_045512.2 14110 . C T 123.0 PASS DP=107;AF=0.056075;SB=3;DP4=46,55,4,2;EFF=STOP_GAINED(HIGH|NONSENSE|Caa/Taa|Q4616*|7096|ORF1ab|protein_coding|CODING|GU280_gp01|2|T),STOP_GAINED(HIGH|NONSENSE|Caa/Taa|Q224*|931|ORF1ab|protein_coding|CODING|YP_009725307.1|2|T|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 14279 . TA T 86.0 PASS DP=107;AF=0.037383;SB=9;DP4=53,51,0,4;INDEL;HRUN=4;EFF=FRAME_SHIFT(HIGH||aaa/|K4673|7096|ORF1ab|protein_coding|CODING|GU280_gp01|2|T|INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||aaa/|K281|931|ORF1ab|protein_coding|CODING|YP_009725307.1|2|T|WARNING_TRANSCRIPT_NO_START_CODON&INFO_REALIGN_3_PRIME);LOF=(ORF1ab|GU280_gp01|28|0.07) -NC_045512.2 14408 . C T 3370.0 PASS DP=94;AF=1.000000;SB=0;DP4=0,0,47,47;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cCt/cTt|P4715L|7096|ORF1ab|protein_coding|CODING|GU280_gp01|2|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cCt/cTt|P323L|931|ORF1ab|protein_coding|CODING|YP_009725307.1|2|T|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 14543 . T C 81.0 PASS DP=69;AF=0.057971;SB=2;DP4=33,32,1,3;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|tTt/tCt|F4760S|7096|ORF1ab|protein_coding|CODING|GU280_gp01|2|C),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|tTt/tCt|F368S|931|ORF1ab|protein_coding|CODING|YP_009725307.1|2|C|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 14675 . C T 132.0 PASS DP=79;AF=0.075949;SB=1;DP4=41,32,4,2;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cCc/cTc|P4804L|7096|ORF1ab|protein_coding|CODING|GU280_gp01|2|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cCc/cTc|P412L|931|ORF1ab|protein_coding|CODING|YP_009725307.1|2|T|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 14750 . TA T 93.0 PASS DP=89;AF=0.044944;SB=0;DP4=39,47,2,2;INDEL;HRUN=4;EFF=FRAME_SHIFT(HIGH||aaa/|K4830|7096|ORF1ab|protein_coding|CODING|GU280_gp01|2|T|INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||aaa/|K438|931|ORF1ab|protein_coding|CODING|YP_009725307.1|2|T|WARNING_TRANSCRIPT_NO_START_CODON&INFO_REALIGN_3_PRIME);LOF=(ORF1ab|GU280_gp01|28|0.07) -NC_045512.2 14772 . G T 106.0 PASS DP=94;AF=0.053191;SB=7;DP4=37,52,4,1;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|caG/caT|Q4836H|7096|ORF1ab|protein_coding|CODING|GU280_gp01|2|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|caG/caT|Q444H|931|ORF1ab|protein_coding|CODING|YP_009725307.1|2|T|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 14835 . T C 154.0 PASS DP=100;AF=0.070000;SB=1;DP4=45,48,4,3;EFF=SYNONYMOUS_CODING(LOW|SILENT|gaT/gaC|D4857|7096|ORF1ab|protein_coding|CODING|GU280_gp01|2|C),SYNONYMOUS_CODING(LOW|SILENT|gaT/gaC|D465|931|ORF1ab|protein_coding|CODING|YP_009725307.1|2|C|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 14840 . GAC G 67.0 PASS DP=97;AF=0.030928;SB=6;DP4=49,46,0,3;INDEL;HRUN=1;EFF=FRAME_SHIFT(HIGH||caa/|Q4860|7096|ORF1ab|protein_coding|CODING|GU280_gp01|2|G|INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||caa/|Q468|931|ORF1ab|protein_coding|CODING|YP_009725307.1|2|G|WARNING_TRANSCRIPT_NO_START_CODON&INFO_REALIGN_3_PRIME);LOF=(ORF1ab|GU280_gp01|28|0.07) -NC_045512.2 15542 . C G 103.0 PASS DP=98;AF=0.051020;SB=1;DP4=50,43,2,3;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aCg/aGg|T5093R|7096|ORF1ab|protein_coding|CODING|GU280_gp01|2|G),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aCg/aGg|T701R|931|ORF1ab|protein_coding|CODING|YP_009725307.1|2|G|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 15579 . CA C 154.0 PASS DP=107;AF=0.065421;SB=1;DP4=55,46,3,4;INDEL;HRUN=4;EFF=FRAME_SHIFT(HIGH||att/|I5107|7096|ORF1ab|protein_coding|CODING|GU280_gp01|2|C|INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||att/|I715|931|ORF1ab|protein_coding|CODING|YP_009725307.1|2|C|WARNING_TRANSCRIPT_NO_START_CODON&INFO_REALIGN_3_PRIME);LOF=(ORF1ab|GU280_gp01|28|0.07) -NC_045512.2 15961 . G T 103.0 PASS DP=98;AF=0.051020;SB=6;DP4=49,44,1,4;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Ggc/Tgc|G5233C|7096|ORF1ab|protein_coding|CODING|GU280_gp01|2|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Ggc/Tgc|G841C|931|ORF1ab|protein_coding|CODING|YP_009725307.1|2|T|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 16111 . C T 3376.0 PASS DP=107;AF=0.897196;SB=0;DP4=5,6,47,49;EFF=SYNONYMOUS_CODING(LOW|SILENT|Cta/Tta|L5283|7096|ORF1ab|protein_coding|CODING|GU280_gp01|2|T),SYNONYMOUS_CODING(LOW|SILENT|Cta/Tta|L891|931|ORF1ab|protein_coding|CODING|YP_009725307.1|2|T|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 16289 . C T 114.0 PASS DP=66;AF=0.075758;SB=0;DP4=27,34,2,3;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gCt/gTt|A5342V|7096|ORF1ab|protein_coding|CODING|GU280_gp01|2|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gCt/gTt|A18V|600|ORF1ab|protein_coding|CODING|YP_009725308.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 16725 . ACAT A 94.0 PASS DP=90;AF=0.044444;SB=2;DP4=40,46,1,3;INDEL;HRUN=1;EFF=CODON_CHANGE_PLUS_CODON_DELETION(MODERATE||catctt/ctt|HL5488L|7096|ORF1ab|protein_coding|CODING|GU280_gp01|2|A|INFO_REALIGN_3_PRIME),CODON_CHANGE_PLUS_CODON_DELETION(MODERATE||catctt/ctt|HL164L|600|ORF1ab|protein_coding|CODING|YP_009725308.1|1|A|WARNING_TRANSCRIPT_NO_START_CODON&INFO_REALIGN_3_PRIME) -NC_045512.2 16837 . GA G 59.0 PASS DP=109;AF=0.027523;SB=2;DP4=57,49,1,2;INDEL;HRUN=5;EFF=FRAME_SHIFT(HIGH||aaa/|K5526|7096|ORF1ab|protein_coding|CODING|GU280_gp01|2|G|INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||aaa/|K202|600|ORF1ab|protein_coding|CODING|YP_009725308.1|1|G|WARNING_TRANSCRIPT_NO_START_CODON&INFO_REALIGN_3_PRIME);LOF=(ORF1ab|GU280_gp01|28|0.07) -NC_045512.2 16851 . TG T 115.0 PASS DP=124;AF=0.040323;SB=1;DP4=66,54,2,3;INDEL;HRUN=2;EFF=FRAME_SHIFT(HIGH||ggt/|G5530|7096|ORF1ab|protein_coding|CODING|GU280_gp01|2|T|INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||ggt/|G206|600|ORF1ab|protein_coding|CODING|YP_009725308.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON&INFO_REALIGN_3_PRIME);LOF=(ORF1ab|GU280_gp01|28|0.07) -NC_045512.2 16864 . G GT 64.0 PASS DP=126;AF=0.023810;SB=2;DP4=67,58,1,2;INDEL;HRUN=3;EFF=FRAME_SHIFT(HIGH||tac/tTac|Y5535L?|7096|ORF1ab|protein_coding|CODING|GU280_gp01|2|GT|INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||tac/tTac|Y211L?|600|ORF1ab|protein_coding|CODING|YP_009725308.1|1|GT|WARNING_TRANSCRIPT_NO_START_CODON&INFO_REALIGN_3_PRIME);LOF=(ORF1ab|GU280_gp01|28|0.07) -NC_045512.2 16887 . C T 76.0 PASS DP=119;AF=0.033613;SB=4;DP4=60,55,1,3;EFF=SYNONYMOUS_CODING(LOW|SILENT|taC/taT|Y5541|7096|ORF1ab|protein_coding|CODING|GU280_gp01|2|T),SYNONYMOUS_CODING(LOW|SILENT|taC/taT|Y217|600|ORF1ab|protein_coding|CODING|YP_009725308.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 16901 . G T 106.0 PASS DP=119;AF=0.050420;SB=3;DP4=58,55,2,4;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gGt/gTt|G5546V|7096|ORF1ab|protein_coding|CODING|GU280_gp01|2|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gGt/gTt|G222V|600|ORF1ab|protein_coding|CODING|YP_009725308.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 17019 . G GT 114.0 PASS DP=116;AF=0.043103;SB=0;DP4=61,51,3,2;INDEL;HRUN=4;EFF=FRAME_SHIFT(HIGH||tct/tTct|S5587F?|7096|ORF1ab|protein_coding|CODING|GU280_gp01|2|GT|INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||tct/tTct|S263F?|600|ORF1ab|protein_coding|CODING|YP_009725308.1|1|GT|WARNING_TRANSCRIPT_NO_START_CODON&INFO_REALIGN_3_PRIME);LOF=(ORF1ab|GU280_gp01|28|0.07) -NC_045512.2 17036 . CA C 117.0 PASS DP=113;AF=0.044248;SB=1;DP4=56,53,2,3;INDEL;HRUN=3;EFF=FRAME_SHIFT(HIGH||aat/|N5592|7096|ORF1ab|protein_coding|CODING|GU280_gp01|2|C|INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||aat/|N268|600|ORF1ab|protein_coding|CODING|YP_009725308.1|1|C|WARNING_TRANSCRIPT_NO_START_CODON&INFO_REALIGN_3_PRIME);LOF=(ORF1ab|GU280_gp01|28|0.07) -NC_045512.2 17143 . CG C 149.0 PASS DP=104;AF=0.067308;SB=0;DP4=49,53,3,4;INDEL;HRUN=1;EFF=FRAME_SHIFT(HIGH||cgc/|R5627|7096|ORF1ab|protein_coding|CODING|GU280_gp01|2|C),FRAME_SHIFT(HIGH||cgc/|R303|600|ORF1ab|protein_coding|CODING|YP_009725308.1|1|C|WARNING_TRANSCRIPT_NO_START_CODON);LOF=(ORF1ab|GU280_gp01|28|0.07) -NC_045512.2 17198 . CATTAAAAT C 97.0 PASS DP=110;AF=0.045455;SB=1;DP4=57,49,2,3;INDEL;HRUN=1;EFF=FRAME_SHIFT(HIGH||ttaaaatatttg/|LKYL5646|7096|ORF1ab|protein_coding|CODING|GU280_gp01|2|C|INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||ttaaaatatttg/|LKYL322|600|ORF1ab|protein_coding|CODING|YP_009725308.1|1|C|WARNING_TRANSCRIPT_NO_START_CODON&INFO_REALIGN_3_PRIME);LOF=(ORF1ab|GU280_gp01|28|0.07) -NC_045512.2 17273 . T C 121.0 PASS DP=123;AF=0.048780;SB=9;DP4=61,56,1,5;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|tTc/tCc|F5670S|7096|ORF1ab|protein_coding|CODING|GU280_gp01|2|C),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|tTc/tCc|F346S|600|ORF1ab|protein_coding|CODING|YP_009725308.1|1|C|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 17288 . C T 92.0 PASS DP=126;AF=0.055556;SB=3;DP4=56,63,2,5;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aCa/aTa|T5675I|7096|ORF1ab|protein_coding|CODING|GU280_gp01|2|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aCa/aTa|T351I|600|ORF1ab|protein_coding|CODING|YP_009725308.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 17639 . C T 73.0 PASS DP=93;AF=0.043011;SB=0;DP4=40,49,2,2;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|tCa/tTa|S5792L|7096|ORF1ab|protein_coding|CODING|GU280_gp01|2|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|tCa/tTa|S468L|600|ORF1ab|protein_coding|CODING|YP_009725308.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 17644 . CA C 68.0 PASS DP=93;AF=0.043011;SB=8;DP4=41,49,0,4;INDEL;HRUN=2;EFF=FRAME_SHIFT(HIGH||caa/|Q5794|7096|ORF1ab|protein_coding|CODING|GU280_gp01|2|C|INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||caa/|Q470|600|ORF1ab|protein_coding|CODING|YP_009725308.1|1|C|WARNING_TRANSCRIPT_NO_START_CODON&INFO_REALIGN_3_PRIME);LOF=(ORF1ab|GU280_gp01|28|0.07) -NC_045512.2 17651 . TTA T 91.0 PASS DP=100;AF=0.050000;SB=11;DP4=45,51,0,5;INDEL;HRUN=3;EFF=FRAME_SHIFT(HIGH||tttaaa/|FK5796|7096|ORF1ab|protein_coding|CODING|GU280_gp01|2|T),FRAME_SHIFT(HIGH||tttaaa/|FK472|600|ORF1ab|protein_coding|CODING|YP_009725308.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON);LOF=(ORF1ab|GU280_gp01|28|0.07) -NC_045512.2 17766 . C T 104.0 PASS DP=104;AF=0.048077;SB=12;DP4=51,48,0,5;EFF=SYNONYMOUS_CODING(LOW|SILENT|gtC/gtT|V5834|7096|ORF1ab|protein_coding|CODING|GU280_gp01|2|T),SYNONYMOUS_CODING(LOW|SILENT|gtC/gtT|V510|600|ORF1ab|protein_coding|CODING|YP_009725308.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 17795 . C T 79.0 PASS DP=111;AF=0.036036;SB=0;DP4=54,53,2,2;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gCt/gTt|A5844V|7096|ORF1ab|protein_coding|CODING|GU280_gp01|2|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gCt/gTt|A520V|600|ORF1ab|protein_coding|CODING|YP_009725308.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 17845 . C T 72.0 PASS DP=97;AF=0.041237;SB=4;DP4=50,43,1,3;EFF=STOP_GAINED(HIGH|NONSENSE|Cag/Tag|Q5861*|7096|ORF1ab|protein_coding|CODING|GU280_gp01|2|T),STOP_GAINED(HIGH|NONSENSE|Cag/Tag|Q537*|600|ORF1ab|protein_coding|CODING|YP_009725308.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 17861 . AC A 91.0 PASS DP=101;AF=0.039604;SB=0;DP4=52,45,2,2;INDEL;HRUN=1;EFF=FRAME_SHIFT(HIGH||gac/|D5866|7096|ORF1ab|protein_coding|CODING|GU280_gp01|2|A),FRAME_SHIFT(HIGH||gac/|D542|600|ORF1ab|protein_coding|CODING|YP_009725308.1|1|A|WARNING_TRANSCRIPT_NO_START_CODON);LOF=(ORF1ab|GU280_gp01|28|0.07) -NC_045512.2 18289 . C T 78.0 PASS DP=100;AF=0.040000;SB=2;DP4=45,51,1,3;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Cgt/Tgt|R6009C|7096|ORF1ab|protein_coding|CODING|GU280_gp01|2|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Cgt/Tgt|R84C|526|ORF1ab|protein_coding|CODING|YP_009725309.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 18335 . A T 107.0 PASS DP=87;AF=0.057471;SB=7;DP4=45,37,1,4;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gAa/gTa|E6024V|7096|ORF1ab|protein_coding|CODING|GU280_gp01|2|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gAa/gTa|E99V|526|ORF1ab|protein_coding|CODING|YP_009725309.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 18434 . AT A 65.0 PASS DP=95;AF=0.031579;SB=2;DP4=44,48,2,1;INDEL;HRUN=5;EFF=FRAME_SHIFT(HIGH||tcc/|S6059|7096|ORF1ab|protein_coding|CODING|GU280_gp01|2|A|INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||tcc/|S134|526|ORF1ab|protein_coding|CODING|YP_009725309.1|1|A|WARNING_TRANSCRIPT_NO_START_CODON&INFO_REALIGN_3_PRIME);LOF=(ORF1ab|GU280_gp01|28|0.07) -NC_045512.2 18443 . G T 103.0 PASS DP=95;AF=0.052632;SB=0;DP4=43,47,2,3;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aGa/aTa|R6060I|7096|ORF1ab|protein_coding|CODING|GU280_gp01|2|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aGa/aTa|R135I|526|ORF1ab|protein_coding|CODING|YP_009725309.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 18711 . TG T 92.0 PASS DP=102;AF=0.039216;SB=0;DP4=55,44,2,2;INDEL;HRUN=1;EFF=FRAME_SHIFT(HIGH||gcc/|A6150|7096|ORF1ab|protein_coding|CODING|GU280_gp01|2|T),FRAME_SHIFT(HIGH||gcc/|A225|526|ORF1ab|protein_coding|CODING|YP_009725309.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON);LOF=(ORF1ab|GU280_gp01|28|0.07) -NC_045512.2 18794 . A C 79.0 PASS DP=107;AF=0.037383;SB=0;DP4=49,54,2,2;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aAc/aCc|N6177T|7096|ORF1ab|protein_coding|CODING|GU280_gp01|2|C),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aAc/aCc|N252T|526|ORF1ab|protein_coding|CODING|YP_009725309.1|1|C|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 18799 . CA C 58.0 PASS DP=105;AF=0.028571;SB=0;DP4=49,54,1,2;INDEL;HRUN=3;EFF=FRAME_SHIFT(HIGH||agc/|S6180|7096|ORF1ab|protein_coding|CODING|GU280_gp01|2|C|INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||agc/|S255|526|ORF1ab|protein_coding|CODING|YP_009725309.1|1|C|WARNING_TRANSCRIPT_NO_START_CODON&INFO_REALIGN_3_PRIME);LOF=(ORF1ab|GU280_gp01|28|0.07) -NC_045512.2 19044 . TA T 123.0 PASS DP=86;AF=0.058140;SB=1;DP4=46,35,2,3;INDEL;HRUN=3;EFF=FRAME_SHIFT(HIGH||aaa/|K6261|7096|ORF1ab|protein_coding|CODING|GU280_gp01|2|T|INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||aaa/|K336|526|ORF1ab|protein_coding|CODING|YP_009725309.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON&INFO_REALIGN_3_PRIME);LOF=(ORF1ab|GU280_gp01|28|0.07) -NC_045512.2 19133 . AATT A 144.0 PASS DP=87;AF=0.080460;SB=3;DP4=40,41,5,2;INDEL;HRUN=2;EFF=CODON_CHANGE_PLUS_CODON_DELETION(MODERATE||ttattc/ttc|LF6291F|7096|ORF1ab|protein_coding|CODING|GU280_gp01|2|A|INFO_REALIGN_3_PRIME),CODON_CHANGE_PLUS_CODON_DELETION(MODERATE||ttattc/ttc|LF366F|526|ORF1ab|protein_coding|CODING|YP_009725309.1|1|A|WARNING_TRANSCRIPT_NO_START_CODON&INFO_REALIGN_3_PRIME) -NC_045512.2 19718 . CA C 119.0 PASS DP=95;AF=0.052632;SB=8;DP4=55,36,1,4;INDEL;HRUN=4;EFF=FRAME_SHIFT(HIGH||aaa/|K6486|7096|ORF1ab|protein_coding|CODING|GU280_gp01|2|C|INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||aaa/|K34|345|ORF1ab|protein_coding|CODING|YP_009725310.1|1|C|WARNING_TRANSCRIPT_NO_START_CODON&INFO_REALIGN_3_PRIME);LOF=(ORF1ab|GU280_gp01|28|0.07) -NC_045512.2 20520 . TG T 70.0 PASS DP=78;AF=0.038462;SB=2;DP4=37,39,2,1;INDEL;HRUN=1;EFF=FRAME_SHIFT(HIGH||gat/|D6753|7096|ORF1ab|protein_coding|CODING|GU280_gp01|2|T),FRAME_SHIFT(HIGH||gat/|D301|345|ORF1ab|protein_coding|CODING|YP_009725310.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON);LOF=(ORF1ab|GU280_gp01|28|0.04) -NC_045512.2 20647 . C T 105.0 PASS DP=93;AF=0.053763;SB=4;DP4=43,45,1,4;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Cca/Tca|P6795S|7096|ORF1ab|protein_coding|CODING|GU280_gp01|2|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Cca/Tca|P343S|345|ORF1ab|protein_coding|CODING|YP_009725310.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON) -NC_045512.2 20950 . TC T 67.0 PASS DP=98;AF=0.030612;SB=2;DP4=43,52,2,1;INDEL;HRUN=1;EFF=FRAME_SHIFT(HIGH||tca/|S6896|7096|ORF1ab|protein_coding|CODING|GU280_gp01|2|T),FRAME_SHIFT(HIGH||tca/|S98|297|ORF1ab|protein_coding|CODING|YP_009725311.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON);LOF=(ORF1ab|GU280_gp01|28|0.04) -NC_045512.2 21060 . TA T 148.0 PASS DP=94;AF=0.063830;SB=0;DP4=42,46,3,3;INDEL;HRUN=2;EFF=FRAME_SHIFT(HIGH||aag/|K6933|7096|ORF1ab|protein_coding|CODING|GU280_gp01|2|T|INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||aag/|K135|297|ORF1ab|protein_coding|CODING|YP_009725311.1|1|T|WARNING_TRANSCRIPT_NO_START_CODON&INFO_REALIGN_3_PRIME);LOF=(ORF1ab|GU280_gp01|28|0.04) -NC_045512.2 21964 . A AT 123.0 PASS DP=76;AF=0.065789;SB=0;DP4=36,36,2,3;INDEL;HRUN=4;EFF=FRAME_SHIFT(HIGH||tgt/tTgt|C136L?|1273|S|protein_coding|CODING|GU280_gp02|1|AT|INFO_REALIGN_3_PRIME);LOF=(S|GU280_gp02|1|1.00) -NC_045512.2 22101 . AAG A 88.0 PASS DP=76;AF=0.052632;SB=0;DP4=37,35,2,2;INDEL;HRUN=2;EFF=FRAME_SHIFT(HIGH||gaagga/|EG180|1273|S|protein_coding|CODING|GU280_gp02|1|A);LOF=(S|GU280_gp02|1|1.00) -NC_045512.2 22127 . C T 80.0 PASS DP=80;AF=0.050000;SB=0;DP4=37,39,2,2;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Ctt/Ttt|L189F|1273|S|protein_coding|CODING|GU280_gp02|1|T) -NC_045512.2 22901 . G GT 66.0 PASS DP=104;AF=0.028846;SB=0;DP4=52,50,2,1;INDEL;HRUN=2;EFF=FRAME_SHIFT(HIGH||ggt/gTgt|G447V?|1273|S|protein_coding|CODING|GU280_gp02|1|GT);LOF=(S|GU280_gp02|1|1.00) -NC_045512.2 23105 . T C 125.0 PASS DP=108;AF=0.055556;SB=1;DP4=50,52,4,2;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Ttt/Ctt|F515L|1273|S|protein_coding|CODING|GU280_gp02|1|C) -NC_045512.2 23143 . TA T 94.0 PASS DP=109;AF=0.045872;SB=12;DP4=52,52,5,0;INDEL;HRUN=5;EFF=FRAME_SHIFT(HIGH||aag/|K529|1273|S|protein_coding|CODING|GU280_gp02|1|T|INFO_REALIGN_3_PRIME);LOF=(S|GU280_gp02|1|1.00) -NC_045512.2 23230 . CA C 114.0 PASS DP=103;AF=0.048544;SB=4;DP4=50,50,4,1;INDEL;HRUN=5;EFF=FRAME_SHIFT(HIGH||aag/|K558|1273|S|protein_coding|CODING|GU280_gp02|1|C|INFO_REALIGN_3_PRIME);LOF=(S|GU280_gp02|1|1.00) -NC_045512.2 23333 . TC T 89.0 PASS DP=109;AF=0.036697;SB=0;DP4=53,54,2,2;INDEL;HRUN=1;EFF=FRAME_SHIFT(HIGH||tct/|S591|1273|S|protein_coding|CODING|GU280_gp02|1|T);LOF=(S|GU280_gp02|1|1.00) -NC_045512.2 23370 . A T 79.0 PASS DP=109;AF=0.036697;SB=0;DP4=55,50,2,2;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aAt/aTt|N603I|1273|S|protein_coding|CODING|GU280_gp02|1|T) -NC_045512.2 23403 . A G 3751.0 PASS DP=105;AF=1.000000;SB=0;DP4=0,0,54,51;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gAt/gGt|D614G|1273|S|protein_coding|CODING|GU280_gp02|1|G) -NC_045512.2 23410 . C T 86.0 PASS DP=108;AF=0.046296;SB=1;DP4=52,50,2,3;EFF=SYNONYMOUS_CODING(LOW|SILENT|aaC/aaT|N616|1273|S|protein_coding|CODING|GU280_gp02|1|T) -NC_045512.2 23675 . GT G 123.0 PASS DP=85;AF=0.058824;SB=0;DP4=41,40,2,3;INDEL;HRUN=2;EFF=FRAME_SHIFT(HIGH||gtt/|V705|1273|S|protein_coding|CODING|GU280_gp02|1|G|INFO_REALIGN_3_PRIME);LOF=(S|GU280_gp02|1|1.00) -NC_045512.2 23683 . C T 83.0 PASS DP=87;AF=0.045977;SB=4;DP4=43,40,1,3;EFF=SYNONYMOUS_CODING(LOW|SILENT|taC/taT|Y707|1273|S|protein_coding|CODING|GU280_gp02|1|T) -NC_045512.2 23694 . CT C 148.0 PASS DP=92;AF=0.065217;SB=0;DP4=46,41,3,3;INDEL;HRUN=1;EFF=FRAME_SHIFT(HIGH||tct/|S711|1273|S|protein_coding|CODING|GU280_gp02|1|C);LOF=(S|GU280_gp02|1|1.00) -NC_045512.2 23887 . CA C 196.0 PASS DP=65;AF=0.123077;SB=3;DP4=34,24,6,2;INDEL;HRUN=5;EFF=FRAME_SHIFT(HIGH||aac/|N777|1273|S|protein_coding|CODING|GU280_gp02|1|C|INFO_REALIGN_3_PRIME);LOF=(S|GU280_gp02|1|1.00) -NC_045512.2 24502 . C T 87.0 PASS DP=108;AF=0.046296;SB=0;DP4=55,48,3,2;EFF=SYNONYMOUS_CODING(LOW|SILENT|atC/atT|I980|1273|S|protein_coding|CODING|GU280_gp02|1|T) -NC_045512.2 24768 . C T 98.0 PASS DP=116;AF=0.043103;SB=0;DP4=54,57,2,3;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cCt/cTt|P1069L|1273|S|protein_coding|CODING|GU280_gp02|1|T) -NC_045512.2 24910 . TAC T 143.0 PASS DP=115;AF=0.052174;SB=0;DP4=51,59,3,3;INDEL;HRUN=1;EFF=FRAME_SHIFT(HIGH||aca/|T1117|1273|S|protein_coding|CODING|GU280_gp02|1|T|INFO_REALIGN_3_PRIME);LOF=(S|GU280_gp02|1|1.00) -NC_045512.2 24977 . G C 76.0 PASS DP=111;AF=0.036036;SB=0;DP4=52,55,2,2;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gat/Cat|D1139H|1273|S|protein_coding|CODING|GU280_gp02|1|C) -NC_045512.2 25208 . AT A 265.0 PASS DP=96;AF=0.104167;SB=1;DP4=44,42,6,4;INDEL;HRUN=3;EFF=FRAME_SHIFT(HIGH||tgg/|W1217|1273|S|protein_coding|CODING|GU280_gp02|1|A|INFO_REALIGN_3_PRIME) -NC_045512.2 25472 . AT A 157.0 PASS DP=104;AF=0.067308;SB=0;DP4=50,48,4,3;INDEL;HRUN=4;EFF=FRAME_SHIFT(HIGH||ttt/|F28|275|ORF3a|protein_coding|CODING|GU280_gp03|1|A|INFO_REALIGN_3_PRIME);LOF=(ORF3a|GU280_gp03|1|1.00) -NC_045512.2 25517 . C CT 111.0 PASS DP=99;AF=0.050505;SB=4;DP4=50,44,4,1;INDEL;HRUN=3;EFF=FRAME_SHIFT(HIGH||ttc/ttTc|F43F?|275|ORF3a|protein_coding|CODING|GU280_gp03|1|CT|INFO_REALIGN_3_PRIME);LOF=(ORF3a|GU280_gp03|1|1.00) -NC_045512.2 25563 . G T 3532.0 PASS DP=100;AF=1.000000;SB=0;DP4=0,0,50,50;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|caG/caT|Q57H|275|ORF3a|protein_coding|CODING|GU280_gp03|1|T) -NC_045512.2 25680 . C T 82.0 PASS DP=71;AF=0.056338;SB=4;DP4=29,38,3,1;EFF=SYNONYMOUS_CODING(LOW|SILENT|ctC/ctT|L96|275|ORF3a|protein_coding|CODING|GU280_gp03|1|T) -NC_045512.2 25777 . C T 85.0 PASS DP=79;AF=0.050633;SB=0;DP4=40,35,2,2;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Ctt/Ttt|L129F|275|ORF3a|protein_coding|CODING|GU280_gp03|1|T) -NC_045512.2 26110 . C T 78.0 PASS DP=117;AF=0.034188;SB=0;DP4=56,57,2,2;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Cct/Tct|P240S|275|ORF3a|protein_coding|CODING|GU280_gp03|1|T) -NC_045512.2 26245 . ATG A 90.0 PASS DP=114;AF=0.035088;SB=2;DP4=55,55,3,1;INDEL;HRUN=1;EFF=FRAME_SHIFT+START_LOST(HIGH||atgtac/|MY1|75|E|protein_coding|CODING|GU280_gp04|1|A|INFO_REALIGN_3_PRIME);LOF=(E|GU280_gp04|1|1.00) -NC_045512.2 26257 . G GT 66.0 PASS DP=108;AF=0.027778;SB=2;DP4=51,56,2,1;INDEL;HRUN=3;EFF=FRAME_SHIFT(HIGH||tcg/tTcg|S6F?|75|E|protein_coding|CODING|GU280_gp04|1|GT|INFO_REALIGN_3_PRIME);LOF=(E|GU280_gp04|1|1.00) -NC_045512.2 26573 . TG T 124.0 PASS DP=75;AF=0.066667;SB=0;DP4=33,37,2,3;INDEL;HRUN=1;EFF=FRAME_SHIFT(HIGH||gaa/|E18|222|M|protein_coding|CODING|GU280_gp05|1|T);LOF=(M|GU280_gp05|1|1.00) -NC_045512.2 26622 . C T 80.0 PASS DP=105;AF=0.038095;SB=4;DP4=56,45,1,3;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Ctt/Ttt|L34F|222|M|protein_coding|CODING|GU280_gp05|1|T) -NC_045512.2 26864 . AG A 123.0 PASS DP=78;AF=0.064103;SB=1;DP4=43,31,2,3;INDEL;HRUN=1;EFF=FRAME_SHIFT(HIGH||gaa/|E115|222|M|protein_coding|CODING|GU280_gp05|1|A);LOF=(M|GU280_gp05|1|1.00) -NC_045512.2 27021 . GA G 148.0 PASS DP=95;AF=0.063158;SB=0;DP4=45,45,3,3;INDEL;HRUN=3;EFF=FRAME_SHIFT(HIGH||atc/|I168|222|M|protein_coding|CODING|GU280_gp05|1|G|INFO_REALIGN_3_PRIME);LOF=(M|GU280_gp05|1|1.00) -NC_045512.2 27404 . TTC T 66.0 PASS DP=108;AF=0.027778;SB=6;DP4=50,55,0,3;INDEL;HRUN=2;EFF=FRAME_SHIFT(HIGH||ctt/|L5|121|ORF7a|protein_coding|CODING|GU280_gp07|1|T|INFO_REALIGN_3_PRIME) -NC_045512.2 27583 . G C 93.0 PASS DP=49;AF=0.081633;SB=0;DP4=21,24,2,2;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gct/Cct|A64P|121|ORF7a|protein_coding|CODING|GU280_gp07|1|C) -NC_045512.2 28076 . CG C 145.0 PASS DP=104;AF=0.057692;SB=0;DP4=44,55,3,3;INDEL;HRUN=1;EFF=FRAME_SHIFT(HIGH||gtg/|V62|121|ORF8|protein_coding|CODING|GU280_gp09|1|C);LOF=(ORF8|GU280_gp09|1|1.00) -NC_045512.2 28146 . C T 102.0 PASS DP=100;AF=0.050000;SB=1;DP4=46,49,3,2;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Cct/Tct|P85S|121|ORF8|protein_coding|CODING|GU280_gp09|1|T) -NC_045512.2 28214 . C CT 143.0 PASS DP=98;AF=0.061224;SB=0;DP4=51,42,3,3;INDEL;HRUN=5;EFF=FRAME_SHIFT(HIGH||tta/ttTa|L109F?|121|ORF8|protein_coding|CODING|GU280_gp09|1|CT|INFO_REALIGN_3_PRIME);LOF=(ORF8|GU280_gp09|1|1.00) -NC_045512.2 28292 . C CA 105.0 PASS DP=102;AF=0.049020;SB=0;DP4=42,55,2,3;INDEL;HRUN=4;EFF=FRAME_SHIFT(HIGH||aat/aaAt|N8K?|419|N|protein_coding|CODING|GU280_gp10|1|CA|INFO_REALIGN_3_PRIME) -NC_045512.2 28579 . AG A 65.0 PASS DP=102;AF=0.029412;SB=0;DP4=53,46,2,1;INDEL;HRUN=1;EFF=FRAME_SHIFT(HIGH||gat/|D103|419|N|protein_coding|CODING|GU280_gp10|1|A);LOF=(N|GU280_gp10|1|1.00) -NC_045512.2 28695 . C T 125.0 PASS DP=105;AF=0.057143;SB=6;DP4=50,49,1,5;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aCa/aTa|T141I|419|N|protein_coding|CODING|GU280_gp10|1|T) -NC_045512.2 28796 . G A 110.0 PASS DP=73;AF=0.068493;SB=1;DP4=30,38,3,2;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Ggg/Agg|G175R|419|N|protein_coding|CODING|GU280_gp10|1|A) -NC_045512.2 29074 . ATACAATG A 58.0 PASS DP=101;AF=0.029703;SB=0;DP4=52,46,2,1;INDEL;HRUN=1;EFF=FRAME_SHIFT(HIGH||tacaatgta/|YNV268|419|N|protein_coding|CODING|GU280_gp10|1|A|INFO_REALIGN_3_PRIME);LOF=(N|GU280_gp10|1|1.00) -NC_045512.2 29362 . C T 86.0 PASS DP=62;AF=0.064516;SB=2;DP4=29,29,3,1;EFF=SYNONYMOUS_CODING(LOW|SILENT|ttC/ttT|F363|419|N|protein_coding|CODING|GU280_gp10|1|T) -NC_045512.2 29382 . A G 67.0 PASS DP=50;AF=0.060000;SB=0;DP4=23,24,1,2;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aAg/aGg|K370R|419|N|protein_coding|CODING|GU280_gp10|1|G) diff --git a/workflows/sars-cov-2-variant-calling/sars-cov-2-variation-reporting/.dockstore.yml b/workflows/sars-cov-2-variant-calling/sars-cov-2-variation-reporting/.dockstore.yml index 968427d61..c1890db44 100644 --- a/workflows/sars-cov-2-variant-calling/sars-cov-2-variation-reporting/.dockstore.yml +++ b/workflows/sars-cov-2-variant-calling/sars-cov-2-variation-reporting/.dockstore.yml @@ -1,7 +1,11 @@ version: 1.2 workflows: - name: 'COVID-19-VARIATION-REPORTING' - primaryDescriptorPath: /variation-reporting.ga subclass: Galaxy + publish: true + primaryDescriptorPath: /variation-reporting.ga testParameterFiles: - - /variation-reporting-test.yml + - /variation-reporting-tests.yml + authors: + - name: Wolfgang Maier + orcid: 0000-0002-9464-6640 diff --git a/workflows/sars-cov-2-variant-calling/sars-cov-2-variation-reporting/CHANGELOG.md b/workflows/sars-cov-2-variant-calling/sars-cov-2-variation-reporting/CHANGELOG.md index e54aae6d2..f7f1325f7 100644 --- a/workflows/sars-cov-2-variant-calling/sars-cov-2-variation-reporting/CHANGELOG.md +++ b/workflows/sars-cov-2-variant-calling/sars-cov-2-variation-reporting/CHANGELOG.md @@ -1,5 +1,15 @@ # Changelog +## [0.3.2] 2023-11-28 + +### Automatic update +- `toolshed.g2.bx.psu.edu/repos/iuc/datamash_ops/datamash_ops/1.1.0+galaxy2` was updated to `toolshed.g2.bx.psu.edu/repos/iuc/datamash_ops/datamash_ops/1.8+galaxy0` +- `toolshed.g2.bx.psu.edu/repos/bgruening/split_file_to_collection/split_file_to_collection/0.5.0` was updated to `toolshed.g2.bx.psu.edu/repos/bgruening/split_file_to_collection/split_file_to_collection/0.5.1` + +## [0.3.1] 2023-11-20 + +- Fix author in dockstore + ## [0.3] 2022-10-13 ### Changed diff --git a/workflows/sars-cov-2-variant-calling/sars-cov-2-variation-reporting/variation-reporting-test.yml b/workflows/sars-cov-2-variant-calling/sars-cov-2-variation-reporting/variation-reporting-tests.yml similarity index 100% rename from workflows/sars-cov-2-variant-calling/sars-cov-2-variation-reporting/variation-reporting-test.yml rename to workflows/sars-cov-2-variant-calling/sars-cov-2-variation-reporting/variation-reporting-tests.yml diff --git a/workflows/sars-cov-2-variant-calling/sars-cov-2-variation-reporting/variation-reporting.ga b/workflows/sars-cov-2-variant-calling/sars-cov-2-variation-reporting/variation-reporting.ga index 598284c1f..ffcbe21e9 100644 --- a/workflows/sars-cov-2-variant-calling/sars-cov-2-variation-reporting/variation-reporting.ga +++ b/workflows/sars-cov-2-variant-calling/sars-cov-2-variation-reporting/variation-reporting.ga @@ -11,14 +11,41 @@ "format-version": "0.1", "license": "MIT", "name": "COVID-19: variation analysis reporting", - "release": "0.3", + "release": "0.3.3", "steps": { "0": { - "annotation": "Allele Frequency Filter. This is the minimum allele frequency required for variants to be included in the reports.", + "annotation": "Variation data in VCF format. Can be the output of any of the workflows in https://github.com/galaxyproject/iwc/tree/main/workflows/sars-cov-2-variant-calling", "content_id": null, "errors": null, "id": 0, "input_connections": {}, + "inputs": [ + { + "description": "Variation data in VCF format. Can be the output of any of the workflows in https://github.com/galaxyproject/iwc/tree/main/workflows/sars-cov-2-variant-calling", + "name": "Variation data to report" + } + ], + "label": "Variation data to report", + "name": "Input dataset collection", + "outputs": [], + "position": { + "left": 10.28125, + "top": 34.08333333333334 + }, + "tool_id": null, + "tool_state": "{\"optional\": false, \"format\": [\"vcf\", \"vcf_bgzip\"], \"tag\": null, \"collection_type\": \"list\"}", + "tool_version": null, + "type": "data_collection_input", + "uuid": "577438f0-38b1-4fc1-9145-935566d62347", + "when": null, + "workflow_outputs": [] + }, + "1": { + "annotation": "Allele Frequency Filter. This is the minimum allele frequency required for variants to be included in the reports.", + "content_id": null, + "errors": null, + "id": 1, + "input_connections": {}, "inputs": [ { "description": "Allele Frequency Filter. This is the minimum allele frequency required for variants to be included in the reports.", @@ -29,14 +56,15 @@ "name": "Input parameter", "outputs": [], "position": { - "left": -421.578125, - "top": 368.4583333333333 + "left": 5.3125, + "top": 186.91145833333331 }, "tool_id": null, "tool_state": "{\"default\": 0.05, \"parameter_type\": \"float\", \"optional\": true}", "tool_version": null, "type": "parameter_input", "uuid": "2e5a5b38-c204-45a2-98e2-e113bce5a14b", + "when": null, "workflow_outputs": [ { "label": "af_filter_threshold", @@ -45,11 +73,11 @@ } ] }, - "1": { + "2": { "annotation": "Depth Filter. This is the minimum depth of all alignments at a variant site. ", "content_id": null, "errors": null, - "id": 1, + "id": 2, "input_connections": {}, "inputs": [ { @@ -61,14 +89,15 @@ "name": "Input parameter", "outputs": [], "position": { - "left": -426.109375, - "top": 466.4739583333333 + "left": 0.78125, + "top": 284.9270833333333 }, "tool_id": null, "tool_state": "{\"default\": 1, \"parameter_type\": \"integer\", \"optional\": true}", "tool_version": null, "type": "parameter_input", "uuid": "c7fc64c7-51c7-465b-9184-ca834d4cf6b2", + "when": null, "workflow_outputs": [ { "label": "dp_filter_threshold", @@ -77,32 +106,6 @@ } ] }, - "2": { - "annotation": "Variation data in VCF format. Can be the output of any of the workflows in https://github.com/galaxyproject/iwc/tree/main/workflows/sars-cov-2-variant-calling", - "content_id": null, - "errors": null, - "id": 2, - "input_connections": {}, - "inputs": [ - { - "description": "Variation data in VCF format. Can be the output of any of the workflows in https://github.com/galaxyproject/iwc/tree/main/workflows/sars-cov-2-variant-calling", - "name": "Variation data to report" - } - ], - "label": "Variation data to report", - "name": "Input dataset collection", - "outputs": [], - "position": { - "left": -416.609375, - "top": 215.63020833333334 - }, - "tool_id": null, - "tool_state": "{\"optional\": false, \"format\": [\"vcf\", \"vcf_bgzip\"], \"collection_type\": \"list\"}", - "tool_version": null, - "type": "data_collection_input", - "uuid": "577438f0-38b1-4fc1-9145-935566d62347", - "workflow_outputs": [] - }, "3": { "annotation": "Depth Filter for variant allele. This is the minimum depth of alignments supporting a variant.", "content_id": null, @@ -119,14 +122,15 @@ "name": "Input parameter", "outputs": [], "position": { - "left": -426.890625, - "top": 548.9739583333334 + "left": 0.0, + "top": 367.42708333333337 }, "tool_id": null, "tool_state": "{\"default\": 10, \"parameter_type\": \"integer\", \"optional\": true}", "tool_version": null, "type": "parameter_input", "uuid": "145bbc55-8893-413d-9620-f3d85e65527a", + "when": null, "workflow_outputs": [ { "label": "dp_alt_filter_threshold", @@ -151,14 +155,15 @@ "name": "Input dataset", "outputs": [], "position": { - "left": 263.125, - "top": 219.95833333333334 + "left": 690.015625, + "top": 38.41145833333334 }, "tool_id": null, - "tool_state": "{\"optional\": false, \"format\": [\"tabular\"]}", + "tool_state": "{\"optional\": false, \"format\": [\"tabular\"], \"tag\": null}", "tool_version": null, "type": "data_input", "uuid": "8b89e674-9012-4f66-a59a-ce05d2fe4223", + "when": null, "workflow_outputs": [] }, "5": { @@ -177,14 +182,15 @@ "name": "Input parameter", "outputs": [], "position": { - "left": 2986.6875, - "top": 853.4583333333334 + "left": 3413.578125, + "top": 671.9114583333334 }, "tool_id": null, "tool_state": "{\"default\": 1, \"parameter_type\": \"integer\", \"optional\": true}", "tool_version": null, "type": "parameter_input", "uuid": "5ca7b1a7-e1ee-4b52-aab4-eaf23949e1da", + "when": null, "workflow_outputs": [ { "label": "plot_number_of_clusters", @@ -200,11 +206,16 @@ "id": 6, "input_connections": { "input": { - "id": 2, + "id": 0, "output_name": "output" } }, - "inputs": [], + "inputs": [ + { + "description": "runtime parameter for tool SnpSift Filter", + "name": "input" + } + ], "label": null, "name": "SnpSift Filter", "outputs": [ @@ -214,8 +225,8 @@ } ], "position": { - "left": -180.234375, - "top": 213.63020833333334 + "left": 246.65625, + "top": 32.08333333333334 }, "post_job_actions": {}, "tool_id": "toolshed.g2.bx.psu.edu/repos/iuc/snpsift/snpSift_filter/4.3+t.galaxy1", @@ -225,10 +236,11 @@ "owner": "iuc", "tool_shed": "toolshed.g2.bx.psu.edu" }, - "tool_state": "{\"filter_expression\": {\"type\": \"simple\", \"__current_case__\": 0, \"expr\": \"DP >= 1\"}, \"filtering\": {\"mode\": \"entries\", \"__current_case__\": 0}, \"input\": {\"__class__\": \"ConnectedValue\"}, \"inverse\": \"false\", \"__page__\": null, \"__rerun_remap_job_id__\": null}", + "tool_state": "{\"filter_expression\": {\"type\": \"simple\", \"__current_case__\": 0, \"expr\": \"DP >= 1\"}, \"filtering\": {\"mode\": \"entries\", \"__current_case__\": 0}, \"input\": {\"__class__\": \"ConnectedValue\"}, \"inverse\": false, \"__page__\": null, \"__rerun_remap_job_id__\": null}", "tool_version": "4.3+t.galaxy1", "type": "tool", "uuid": "386eb313-89c1-47df-bd3d-976780eff740", + "when": null, "workflow_outputs": [ { "label": "prefiltered_variants", @@ -244,11 +256,11 @@ "id": 7, "input_connections": { "components_1|param_type|component_value": { - "id": 0, + "id": 1, "output_name": "output" }, "components_3|param_type|component_value": { - "id": 1, + "id": 2, "output_name": "output" }, "components_5|param_type|component_value": { @@ -266,8 +278,8 @@ } ], "position": { - "left": -181.890625, - "top": 337.9114583333333 + "left": 245.0, + "top": 156.36458333333331 }, "post_job_actions": { "HideDatasetActionout1": { @@ -287,6 +299,7 @@ "tool_version": "0.1.1", "type": "tool", "uuid": "ec1a4872-b9bc-41f0-8675-1f26fdd19f16", + "when": null, "workflow_outputs": [] }, "8": { @@ -296,11 +309,11 @@ "id": 8, "input_connections": { "components_1|param_type|component_value": { - "id": 0, + "id": 1, "output_name": "output" }, "components_3|param_type|component_value": { - "id": 1, + "id": 2, "output_name": "output" }, "components_5|param_type|component_value": { @@ -318,8 +331,8 @@ } ], "position": { - "left": -185.171875, - "top": 645.3802083333334 + "left": 241.71875, + "top": 463.83333333333337 }, "post_job_actions": { "HideDatasetActionout1": { @@ -339,6 +352,7 @@ "tool_version": "0.1.1", "type": "tool", "uuid": "c9916563-c914-4f5f-90c6-d6c7619e1a98", + "when": null, "workflow_outputs": [] }, "9": { @@ -360,7 +374,20 @@ "output_name": "output" } }, - "inputs": [], + "inputs": [ + { + "description": "runtime parameter for tool SnpSift Filter", + "name": "filter_expression" + }, + { + "description": "runtime parameter for tool SnpSift Filter", + "name": "filtering" + }, + { + "description": "runtime parameter for tool SnpSift Filter", + "name": "input" + } + ], "label": null, "name": "SnpSift Filter", "outputs": [ @@ -370,8 +397,8 @@ } ], "position": { - "left": 50.53125, - "top": 503.6145833333333 + "left": 477.421875, + "top": 322.0677083333333 }, "post_job_actions": { "RenameDatasetActionoutput": { @@ -389,10 +416,11 @@ "owner": "iuc", "tool_shed": "toolshed.g2.bx.psu.edu" }, - "tool_state": "{\"filter_expression\": {\"type\": \"simple\", \"__current_case__\": 0, \"expr\": {\"__class__\": \"ConnectedValue\"}}, \"filtering\": {\"mode\": \"add_filter\", \"__current_case__\": 3, \"add_filter\": {\"__class__\": \"ConnectedValue\"}}, \"input\": {\"__class__\": \"ConnectedValue\"}, \"inverse\": \"false\", \"__page__\": null, \"__rerun_remap_job_id__\": null}", + "tool_state": "{\"filter_expression\": {\"type\": \"simple\", \"__current_case__\": 0, \"expr\": {\"__class__\": \"ConnectedValue\"}}, \"filtering\": {\"mode\": \"add_filter\", \"__current_case__\": 3, \"add_filter\": {\"__class__\": \"ConnectedValue\"}}, \"input\": {\"__class__\": \"ConnectedValue\"}, \"inverse\": false, \"__page__\": null, \"__rerun_remap_job_id__\": null}", "tool_version": "4.3+t.galaxy1", "type": "tool", "uuid": "9a6cb40d-476b-4096-adcc-cb601398d0b4", + "when": null, "workflow_outputs": [ { "label": "filtered_variants", @@ -412,7 +440,12 @@ "output_name": "output" } }, - "inputs": [], + "inputs": [ + { + "description": "runtime parameter for tool SnpSift Extract Fields", + "name": "input" + } + ], "label": null, "name": "SnpSift Extract Fields", "outputs": [ @@ -422,8 +455,8 @@ } ], "position": { - "left": 286.546875, - "top": 400.5989583333333 + "left": 713.4375, + "top": 219.05208333333331 }, "post_job_actions": { "RenameDatasetActionoutput": { @@ -441,10 +474,11 @@ "owner": "iuc", "tool_shed": "toolshed.g2.bx.psu.edu" }, - "tool_state": "{\"empty_text\": \".\", \"extract\": \"CHROM POS FILTER REF ALT DP AF DP4 SB EFF[*].IMPACT EFF[*].FUNCLASS EFF[*].EFFECT EFF[*].GENE EFF[*].CODON EFF[*].AA EFF[*].TRID DP4[2] DP4[3]\", \"input\": {\"__class__\": \"ConnectedValue\"}, \"one_effect_per_line\": \"true\", \"separator\": \"\", \"__page__\": null, \"__rerun_remap_job_id__\": null}", + "tool_state": "{\"empty_text\": \".\", \"extract\": \"CHROM POS FILTER REF ALT DP AF DP4 SB EFF[*].IMPACT EFF[*].FUNCLASS EFF[*].EFFECT EFF[*].GENE EFF[*].CODON EFF[*].AA EFF[*].TRID DP4[2] DP4[3]\", \"input\": {\"__class__\": \"ConnectedValue\"}, \"one_effect_per_line\": true, \"separator\": \"\", \"__page__\": null, \"__rerun_remap_job_id__\": null}", "tool_version": "4.3+t.galaxy0", "type": "tool", "uuid": "085e8f20-3eea-4d97-8e52-6e8a9aa3fbd7", + "when": null, "workflow_outputs": [ { "label": "filtered_extracted_variants", @@ -468,7 +502,16 @@ "output_name": "output" } }, - "inputs": [], + "inputs": [ + { + "description": "runtime parameter for tool Replace column", + "name": "original_file" + }, + { + "description": "runtime parameter for tool Replace column", + "name": "replace_information" + } + ], "label": null, "name": "Replace column", "outputs": [ @@ -478,8 +521,8 @@ } ], "position": { - "left": 517.421875, - "top": 264.5833333333333 + "left": 944.3125, + "top": 83.03645833333331 }, "post_job_actions": { "ChangeDatatypeActionoutfile_replace": { @@ -508,6 +551,7 @@ "tool_version": "0.2", "type": "tool", "uuid": "0850f6d5-ff1f-47f9-8e49-b7eb5782828a", + "when": null, "workflow_outputs": [ { "label": "filtered_and_renamed_effects", @@ -542,8 +586,8 @@ } ], "position": { - "left": 737.84375, - "top": 421.8958333333333 + "left": 1164.734375, + "top": 240.34895833333331 }, "post_job_actions": { "RenameDatasetActionout_file1": { @@ -561,10 +605,11 @@ "owner": "devteam", "tool_shed": "toolshed.g2.bx.psu.edu" }, - "tool_state": "{\"avoid_scientific_notation\": \"false\", \"error_handling\": {\"auto_col_types\": \"true\", \"fail_on_non_existent_columns\": \"true\", \"non_computable\": {\"action\": \"--fail-on-non-computable\", \"__current_case__\": 0}}, \"input\": {\"__class__\": \"RuntimeValue\"}, \"ops\": {\"header_lines_select\": \"yes\", \"__current_case__\": 1, \"expressions\": [{\"__index__\": 0, \"cond\": \"c7\", \"add_column\": {\"mode\": \"I\", \"__current_case__\": 1, \"pos\": \"8\"}, \"new_column_name\": \"AFcaller\"}, {\"__index__\": 1, \"cond\": \"round((c18 + c19) / c6, 6)\", \"add_column\": {\"mode\": \"R\", \"__current_case__\": 2, \"pos\": \"7\"}, \"new_column_name\": \"AF\"}]}, \"__page__\": null, \"__rerun_remap_job_id__\": null}", + "tool_state": "{\"avoid_scientific_notation\": false, \"error_handling\": {\"auto_col_types\": true, \"fail_on_non_existent_columns\": true, \"non_computable\": {\"action\": \"--fail-on-non-computable\", \"__current_case__\": 0}}, \"input\": {\"__class__\": \"ConnectedValue\"}, \"ops\": {\"header_lines_select\": \"yes\", \"__current_case__\": 1, \"expressions\": [{\"__index__\": 0, \"cond\": \"c7\", \"add_column\": {\"mode\": \"I\", \"__current_case__\": 1, \"pos\": \"8\"}, \"new_column_name\": \"AFcaller\"}, {\"__index__\": 1, \"cond\": \"round((c18 + c19) / c6, 6)\", \"add_column\": {\"mode\": \"R\", \"__current_case__\": 2, \"pos\": \"7\"}, \"new_column_name\": \"AF\"}]}, \"__page__\": null, \"__rerun_remap_job_id__\": null}", "tool_version": "2.0", "type": "tool", "uuid": "c457f29e-dea5-449a-84c4-4b192da88257", + "when": null, "workflow_outputs": [ { "label": "af_recalculated", @@ -575,7 +620,7 @@ }, "13": { "annotation": "", - "content_id": "toolshed.g2.bx.psu.edu/repos/iuc/datamash_ops/datamash_ops/1.1.0+galaxy2", + "content_id": "toolshed.g2.bx.psu.edu/repos/iuc/datamash_ops/datamash_ops/1.8+galaxy0", "errors": null, "id": 13, "input_connections": { @@ -584,18 +629,23 @@ "output_name": "out_file1" } }, - "inputs": [], + "inputs": [ + { + "description": "runtime parameter for tool Datamash", + "name": "in_file" + } + ], "label": null, "name": "Datamash", "outputs": [ { "name": "out_file", - "type": "tabular" + "type": "input" } ], "position": { - "left": 904.265625, - "top": 202.13020833333334 + "left": 1331.15625, + "top": 20.583333333333343 }, "post_job_actions": { "RenameDatasetActionout_file": { @@ -606,17 +656,18 @@ "output_name": "out_file" } }, - "tool_id": "toolshed.g2.bx.psu.edu/repos/iuc/datamash_ops/datamash_ops/1.1.0+galaxy2", + "tool_id": "toolshed.g2.bx.psu.edu/repos/iuc/datamash_ops/datamash_ops/1.8+galaxy0", "tool_shed_repository": { - "changeset_revision": "746e8e4bf929", + "changeset_revision": "4c07ddedc198", "name": "datamash_ops", "owner": "iuc", "tool_shed": "toolshed.g2.bx.psu.edu" }, - "tool_state": "{\"grouping\": \"1,2,3,4,5,6,7,8,9,10\", \"header_in\": \"true\", \"header_out\": \"true\", \"ignore_case\": \"false\", \"in_file\": {\"__class__\": \"ConnectedValue\"}, \"narm\": \"false\", \"need_sort\": \"false\", \"operations\": [{\"__index__\": 0, \"op_name\": \"collapse\", \"op_column\": \"11\"}, {\"__index__\": 1, \"op_name\": \"collapse\", \"op_column\": \"12\"}, {\"__index__\": 2, \"op_name\": \"collapse\", \"op_column\": \"13\"}, {\"__index__\": 3, \"op_name\": \"collapse\", \"op_column\": \"14\"}, {\"__index__\": 4, \"op_name\": \"collapse\", \"op_column\": \"15\"}, {\"__index__\": 5, \"op_name\": \"collapse\", \"op_column\": \"16\"}, {\"__index__\": 6, \"op_name\": \"collapse\", \"op_column\": \"17\"}], \"print_full_line\": \"false\", \"__page__\": null, \"__rerun_remap_job_id__\": null}", - "tool_version": "1.1.0+galaxy2", + "tool_state": "{\"grouping\": \"1,2,3,4,5,6,7,8,9,10\", \"header_in\": true, \"header_out\": true, \"ignore_case\": false, \"in_file\": {\"__class__\": \"ConnectedValue\"}, \"narm\": false, \"need_sort\": false, \"operations\": [{\"__index__\": 0, \"op_name\": \"collapse\", \"op_column\": \"11\"}, {\"__index__\": 1, \"op_name\": \"collapse\", \"op_column\": \"12\"}, {\"__index__\": 2, \"op_name\": \"collapse\", \"op_column\": \"13\"}, {\"__index__\": 3, \"op_name\": \"collapse\", \"op_column\": \"14\"}, {\"__index__\": 4, \"op_name\": \"collapse\", \"op_column\": \"15\"}, {\"__index__\": 5, \"op_name\": \"collapse\", \"op_column\": \"16\"}, {\"__index__\": 6, \"op_name\": \"collapse\", \"op_column\": \"17\"}], \"print_full_line\": false, \"__page__\": null, \"__rerun_remap_job_id__\": null}", + "tool_version": "1.8+galaxy0", "type": "tool", "uuid": "ec8aca39-fe1f-404a-9460-7a960cec37e6", + "when": null, "workflow_outputs": [ { "label": "collapsed_effects", @@ -627,7 +678,7 @@ }, "14": { "annotation": "", - "content_id": "toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_find_and_replace/1.1.4", + "content_id": "toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_find_and_replace/9.3+galaxy0", "errors": null, "id": 14, "input_connections": { @@ -636,7 +687,12 @@ "output_name": "out_file" } }, - "inputs": [], + "inputs": [ + { + "description": "runtime parameter for tool Replace", + "name": "infile" + } + ], "label": null, "name": "Replace", "outputs": [ @@ -646,8 +702,8 @@ } ], "position": { - "left": 1254.34375, - "top": 181.546875 + "left": 1681.234375, + "top": 0.0 }, "post_job_actions": { "RenameDatasetActionoutfile": { @@ -658,17 +714,18 @@ "output_name": "outfile" } }, - "tool_id": "toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_find_and_replace/1.1.4", + "tool_id": "toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_find_and_replace/9.3+galaxy0", "tool_shed_repository": { - "changeset_revision": "d698c222f354", + "changeset_revision": "12615d397df7", "name": "text_processing", "owner": "bgruening", "tool_shed": "toolshed.g2.bx.psu.edu" }, - "tool_state": "{\"find_and_replace\": [{\"__index__\": 0, \"find_pattern\": \"\\\\t([^\\\\t,]+),[^\\\\t]+\\\\t([^\\\\t,]+),[^\\\\t]+\\\\t([^\\\\t,]+),[^\\\\t]+\\\\t([^\\\\t,]+),[^\\\\t]+\\\\t([^\\\\t,]+),[^\\\\t]+\\\\t([^\\\\t,]+),[^\\\\t]+\\\\t([^\\\\t,]+),[^\\\\s]+\", \"replace_pattern\": \"\\\\t$1\\\\t$2\\\\t$3\\\\t$4\\\\t$5\\\\t$6\\\\t$7\", \"is_regex\": \"true\", \"global\": \"true\", \"caseinsensitive\": \"false\", \"wholewords\": \"false\", \"skip_first_line\": \"true\", \"searchwhere\": {\"searchwhere_select\": \"line\", \"__current_case__\": 0}}, {\"__index__\": 1, \"find_pattern\": \"(GroupBy|collapse)\\\\(([^)]+)\\\\)\", \"replace_pattern\": \"$2\", \"is_regex\": \"true\", \"global\": \"true\", \"caseinsensitive\": \"false\", \"wholewords\": \"false\", \"skip_first_line\": \"false\", \"searchwhere\": {\"searchwhere_select\": \"line\", \"__current_case__\": 0}}], \"infile\": {\"__class__\": \"ConnectedValue\"}, \"__page__\": null, \"__rerun_remap_job_id__\": null}", - "tool_version": "1.1.4", + "tool_state": "{\"find_and_replace\": [{\"__index__\": 0, \"find_pattern\": \"\\\\t([^\\\\t,]+),[^\\\\t]+\\\\t([^\\\\t,]+),[^\\\\t]+\\\\t([^\\\\t,]+),[^\\\\t]+\\\\t([^\\\\t,]+),[^\\\\t]+\\\\t([^\\\\t,]+),[^\\\\t]+\\\\t([^\\\\t,]+),[^\\\\t]+\\\\t([^\\\\t,]+),[^\\\\s]+\", \"replace_pattern\": \"\\\\t$1\\\\t$2\\\\t$3\\\\t$4\\\\t$5\\\\t$6\\\\t$7\", \"is_regex\": true, \"global\": true, \"caseinsensitive\": false, \"wholewords\": false, \"skip_first_line\": true, \"searchwhere\": {\"searchwhere_select\": \"line\", \"__current_case__\": 0}}, {\"__index__\": 1, \"find_pattern\": \"(GroupBy|collapse)\\\\(([^)]+)\\\\)\", \"replace_pattern\": \"$2\", \"is_regex\": true, \"global\": true, \"caseinsensitive\": false, \"wholewords\": false, \"skip_first_line\": false, \"searchwhere\": {\"searchwhere_select\": \"line\", \"__current_case__\": 0}}], \"infile\": {\"__class__\": \"ConnectedValue\"}, \"__page__\": null, \"__rerun_remap_job_id__\": null}", + "tool_version": "9.3+galaxy0", "type": "tool", "uuid": "25f665b6-2b6a-49a3-a395-29706cd772ab", + "when": null, "workflow_outputs": [ { "label": "highest_impact_effects", @@ -688,7 +745,12 @@ "output_name": "outfile" } }, - "inputs": [], + "inputs": [ + { + "description": "runtime parameter for tool Collapse Collection", + "name": "input_list" + } + ], "label": null, "name": "Collapse Collection", "outputs": [ @@ -698,8 +760,8 @@ } ], "position": { - "left": 1555.609375, - "top": 209.61458333333334 + "left": 1982.5, + "top": 28.067708333333343 }, "post_job_actions": { "HideDatasetActionoutput": { @@ -715,10 +777,11 @@ "owner": "nml", "tool_shed": "toolshed.g2.bx.psu.edu" }, - "tool_state": "{\"filename\": {\"add_name\": \"true\", \"__current_case__\": 0, \"place_name\": \"same_multiple\"}, \"input_list\": {\"__class__\": \"ConnectedValue\"}, \"one_header\": \"true\", \"__page__\": null, \"__rerun_remap_job_id__\": null}", + "tool_state": "{\"filename\": {\"add_name\": true, \"__current_case__\": 0, \"place_name\": \"same_multiple\"}, \"input_list\": {\"__class__\": \"ConnectedValue\"}, \"one_header\": true, \"__page__\": null, \"__rerun_remap_job_id__\": null}", "tool_version": "5.1.0", "type": "tool", "uuid": "e5459c74-11e1-45a5-beee-88c2dde61eab", + "when": null, "workflow_outputs": [] }, "16": { @@ -732,7 +795,12 @@ "output_name": "output" } }, - "inputs": [], + "inputs": [ + { + "description": "runtime parameter for tool Compute", + "name": "input" + } + ], "label": null, "name": "Compute", "outputs": [ @@ -742,8 +810,8 @@ } ], "position": { - "left": 1801, - "top": 393.9270833333333 + "left": 2227.890625, + "top": 212.38020833333331 }, "post_job_actions": { "HideDatasetActionout_file1": { @@ -759,15 +827,16 @@ "owner": "devteam", "tool_shed": "toolshed.g2.bx.psu.edu" }, - "tool_state": "{\"avoid_scientific_notation\": \"false\", \"error_handling\": {\"auto_col_types\": \"false\", \"fail_on_non_existent_columns\": \"true\", \"non_computable\": {\"action\": \"--fail-on-non-computable\", \"__current_case__\": 0}}, \"input\": {\"__class__\": \"RuntimeValue\"}, \"ops\": {\"header_lines_select\": \"yes\", \"__current_case__\": 1, \"expressions\": [{\"__index__\": 0, \"cond\": \"c5 + '>' + c6\", \"add_column\": {\"mode\": \"\", \"__current_case__\": 0, \"pos\": \"\"}, \"new_column_name\": \"change\"}, {\"__index__\": 1, \"cond\": \"c3 + ':' + c19\", \"add_column\": {\"mode\": \"\", \"__current_case__\": 0, \"pos\": \"\"}, \"new_column_name\": \"change_with_pos\"}]}, \"__page__\": null, \"__rerun_remap_job_id__\": null}", + "tool_state": "{\"avoid_scientific_notation\": false, \"error_handling\": {\"auto_col_types\": false, \"fail_on_non_existent_columns\": true, \"non_computable\": {\"action\": \"--fail-on-non-computable\", \"__current_case__\": 0}}, \"input\": {\"__class__\": \"ConnectedValue\"}, \"ops\": {\"header_lines_select\": \"yes\", \"__current_case__\": 1, \"expressions\": [{\"__index__\": 0, \"cond\": \"c5 + '>' + c6\", \"add_column\": {\"mode\": \"\", \"__current_case__\": 0, \"pos\": \"\"}, \"new_column_name\": \"change\"}, {\"__index__\": 1, \"cond\": \"c3 + ':' + c19\", \"add_column\": {\"mode\": \"\", \"__current_case__\": 0, \"pos\": \"\"}, \"new_column_name\": \"change_with_pos\"}]}, \"__page__\": null, \"__rerun_remap_job_id__\": null}", "tool_version": "2.0", "type": "tool", "uuid": "cc8a768f-8078-4bdd-ae98-903f08619070", + "when": null, "workflow_outputs": [] }, "17": { "annotation": "", - "content_id": "toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_find_and_replace/1.1.4", + "content_id": "toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_find_and_replace/9.3+galaxy0", "errors": null, "id": 17, "input_connections": { @@ -776,7 +845,12 @@ "output_name": "out_file1" } }, - "inputs": [], + "inputs": [ + { + "description": "runtime parameter for tool Replace", + "name": "infile" + } + ], "label": null, "name": "Replace", "outputs": [ @@ -785,9 +859,9 @@ "type": "input" } ], - "position": { - "left": 2007.171875, - "top": 261.7239583333333 + "position": { + "left": 2434.0625, + "top": 80.17708333333331 }, "post_job_actions": { "RenameDatasetActionoutfile": { @@ -798,17 +872,18 @@ "output_name": "outfile" } }, - "tool_id": "toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_find_and_replace/1.1.4", + "tool_id": "toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_find_and_replace/9.3+galaxy0", "tool_shed_repository": { - "changeset_revision": "d698c222f354", + "changeset_revision": "12615d397df7", "name": "text_processing", "owner": "bgruening", "tool_shed": "toolshed.g2.bx.psu.edu" }, - "tool_state": "{\"find_and_replace\": [{\"__index__\": 0, \"caseinsensitive\": \"false\", \"find_pattern\": \"EFF[*].\", \"global\": \"true\", \"infile\": {\"__class__\": \"ConnectedValue\"}, \"is_regex\": \"false\", \"replace_pattern\": \"\", \"searchwhere\": {\"searchwhere_select\": \"line\", \"__current_case__\": 0}, \"skip_first_line\": \"false\", \"wholewords\": \"false\"}], \"infile\": {\"__class__\": \"ConnectedValue\"}, \"__page__\": null, \"__rerun_remap_job_id__\": null}", - "tool_version": "1.1.4", + "tool_state": "{\"find_and_replace\": [{\"__index__\": 0, \"find_pattern\": \"EFF[*].\", \"replace_pattern\": \"\", \"is_regex\": false, \"global\": true, \"caseinsensitive\": false, \"wholewords\": false, \"skip_first_line\": false, \"searchwhere\": {\"searchwhere_select\": \"line\", \"__current_case__\": 0}}], \"infile\": {\"__class__\": \"ConnectedValue\"}, \"__page__\": null, \"__rerun_remap_job_id__\": null}", + "tool_version": "9.3+galaxy0", "type": "tool", "uuid": "82276b6c-5c6e-49eb-8ee5-46041c273d30", + "when": null, "workflow_outputs": [ { "label": "all_variants_all_samples", @@ -819,7 +894,7 @@ }, "18": { "annotation": "", - "content_id": "toolshed.g2.bx.psu.edu/repos/iuc/datamash_ops/datamash_ops/1.1.0+galaxy2", + "content_id": "toolshed.g2.bx.psu.edu/repos/iuc/datamash_ops/datamash_ops/1.8+galaxy0", "errors": null, "id": 18, "input_connections": { @@ -839,12 +914,12 @@ "outputs": [ { "name": "out_file", - "type": "tabular" + "type": "input" } ], "position": { - "left": 2258.4375, - "top": 213.14583333333334 + "left": 2685.328125, + "top": 31.598958333333343 }, "post_job_actions": { "HideDatasetActionout_file": { @@ -853,17 +928,18 @@ "output_name": "out_file" } }, - "tool_id": "toolshed.g2.bx.psu.edu/repos/iuc/datamash_ops/datamash_ops/1.1.0+galaxy2", + "tool_id": "toolshed.g2.bx.psu.edu/repos/iuc/datamash_ops/datamash_ops/1.8+galaxy0", "tool_shed_repository": { - "changeset_revision": "746e8e4bf929", + "changeset_revision": "4c07ddedc198", "name": "datamash_ops", "owner": "iuc", "tool_shed": "toolshed.g2.bx.psu.edu" }, - "tool_state": "{\"grouping\": \"20\", \"header_in\": \"true\", \"header_out\": \"true\", \"ignore_case\": \"false\", \"in_file\": {\"__class__\": \"ConnectedValue\"}, \"narm\": \"false\", \"need_sort\": \"true\", \"operations\": [{\"__index__\": 0, \"op_name\": \"countunique\", \"op_column\": \"1\"}, {\"__index__\": 1, \"op_name\": \"min\", \"op_column\": \"8\"}, {\"__index__\": 2, \"op_name\": \"max\", \"op_column\": \"8\"}, {\"__index__\": 3, \"op_name\": \"collapse\", \"op_column\": \"1\"}, {\"__index__\": 4, \"op_name\": \"collapse\", \"op_column\": \"8\"}], \"print_full_line\": \"false\", \"__page__\": null, \"__rerun_remap_job_id__\": null}", - 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"uuid": "0b2f751b-03c6-4302-9060-16856bb4157c", + "uuid": "583b8d59-2cc0-468e-a0b7-3b1a788c7613", + "when": null, "workflow_outputs": [ { - "label": "by_variant_report", + "label": "variant_frequency_plot", "output_name": "outfile", - "uuid": "bbb19b95-70e3-43fb-baae-525f752b86e9" + "uuid": "986f1d1a-02c0-4d91-9f6e-a09a088732ee" } ] } @@ -1668,4 +1866,4 @@ "covid19.galaxyproject.org" ], "uuid": "b08c744d-7c61-4b58-ac5f-4b5886c3c643" -} +} \ No newline at end of file diff --git a/workflows/scRNAseq/baredsc/.dockstore.yml b/workflows/scRNAseq/baredsc/.dockstore.yml new file mode 100644 index 000000000..3073aa73e --- /dev/null +++ b/workflows/scRNAseq/baredsc/.dockstore.yml @@ -0,0 +1,20 @@ +version: 1.2 +workflows: +- name: baredSC-1d-logNorm + subclass: Galaxy + publish: true + primaryDescriptorPath: /baredSC-1d-logNorm.ga + testParameterFiles: + - /baredSC-1d-logNorm-tests.yml + authors: + - name: Lucille Delisle + orcid: 0000-0002-1964-4960 +- name: baredSC-2d-logNorm + subclass: Galaxy + publish: true + primaryDescriptorPath: /baredSC-2d-logNorm.ga + testParameterFiles: + - /baredSC-2d-logNorm-tests.yml + authors: + - name: Lucille Delisle + orcid: 0000-0002-1964-4960 diff --git a/workflows/scRNAseq/baredsc/CHANGELOG.md b/workflows/scRNAseq/baredsc/CHANGELOG.md new file mode 100644 index 000000000..37435d618 --- /dev/null +++ b/workflows/scRNAseq/baredsc/CHANGELOG.md @@ -0,0 +1,30 @@ +# Changelog + +## [0.4] 2024-04-08 + +### Automatic update +- `toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_text_file_with_recurring_lines/9.3+galaxy0` was updated to `toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_text_file_with_recurring_lines/9.3+galaxy1` +- `toolshed.g2.bx.psu.edu/repos/iuc/baredsc_1d/baredsc_1d/1.1.2+galaxy1` was updated to `toolshed.g2.bx.psu.edu/repos/iuc/baredsc_1d/baredsc_1d/1.1.3+galaxy0` +- `toolshed.g2.bx.psu.edu/repos/iuc/baredsc_combine_1d/baredsc_combine_1d/1.1.2+galaxy1` was updated to `toolshed.g2.bx.psu.edu/repos/iuc/baredsc_combine_1d/baredsc_combine_1d/1.1.3+galaxy0` +- `toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_text_file_with_recurring_lines/9.3+galaxy0` was updated to `toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_text_file_with_recurring_lines/9.3+galaxy1` +- `toolshed.g2.bx.psu.edu/repos/iuc/baredsc_2d/baredsc_2d/1.1.2+galaxy1` was updated to `toolshed.g2.bx.psu.edu/repos/iuc/baredsc_2d/baredsc_2d/1.1.3+galaxy0` +- `toolshed.g2.bx.psu.edu/repos/iuc/baredsc_combine_2d/baredsc_combine_2d/1.1.2+galaxy1` was updated to `toolshed.g2.bx.psu.edu/repos/iuc/baredsc_combine_2d/baredsc_combine_2d/1.1.3+galaxy0` + +## [0.3] 2024-03-05 + +### Automatic update +- `toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_text_file_with_recurring_lines/1.1.0` was updated to `toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_text_file_with_recurring_lines/9.3+galaxy0` + +## [0.2] 2023-12-01 + +### Add 'compute p-value' parameter in 2d + +### Semi-automatic update +- all baredsc tools were updated from `1.1.2+galaxy0` to `1.1.2+galaxy1` + +### Automatic update +- `toolshed.g2.bx.psu.edu/repos/bgruening/split_file_to_collection/split_file_to_collection/0.5.0` was updated to `toolshed.g2.bx.psu.edu/repos/bgruening/split_file_to_collection/split_file_to_collection/0.5.1` + +## [0.1] 2023-10-03 + +First release. diff --git a/workflows/scRNAseq/baredsc/README.md b/workflows/scRNAseq/baredsc/README.md new file mode 100644 index 000000000..bbd9591e8 --- /dev/null +++ b/workflows/scRNAseq/baredsc/README.md @@ -0,0 +1,38 @@ +# BaredSC Workflows + +These workflows allow to run a baredSC analysis from a table with counts in a single click. It uses models from 1 to N Gaussians and combine them. It uses the logNorm scale, 100 bins for 1 dimension and 25 bins on each axis in 2 dimensions. + +## Inputs dataset + +- Both workflows need a tabular dataset where each row is a cell. The tabular needs to have a header line with column names. There must be at least two columns: 'nCount_RNA' and another one with the counts for the gene(s) of interest. A way to get such table in R from a Seurat object (`seurat.obj`) is: + +```r +my.genes <- c("Hoxa13", "Hoxd13") +df <- cbind(seurat.obj[[]], # This will give you all metadata including nCount_RNA + FetchData(seurat.obj, slot = "counts", vars = my.genes)) + +write.table(df, "input_for_baredSC.txt", quote = F, sep = "\t", row.names = F) +``` + +## Inputs values + +For the 1D: + +- Gene name: The name of the column with the counts of your gene of interest. +- Maximum value in logNorm: The maximum value to explore in PDF. This value should be large enough so the PDF is at 0 at this value. +- Maximum number of Gaussians to study: All models between models with 1 Gaussians to models with this number of Gaussians will be combined. + +For the 2D: + +- Gene name for x axis: The name of the column with the counts of your gene in x axis. +- Gene name for y axis: The name of the column with the counts of your gene in y axis. +- maximum value in logNorm for x-axis: The maximum value to explore in PDF in the x axis. This value should be large enough so the PDF is at 0 at this value. +- maximum value in logNorm for y-axis: The maximum value to explore in PDF in the y axis. This value should be large enough so the PDF is at 0 at this value. +- Maximum number of Gaussians to study: All models between models with 1 2D-Gaussians to models with this number of 2D-Gaussians will be combined. +- compute p-value: Whether you want to get a p-value. As a consequence, less samples than available will be used for plots as p-value computation requires to have independent samples. + +## Processing + +- The workflow will generate paramater values from 1 to the maximum number of Gaussians to study. +- baredSC_1d or baredSC_2d is run for each of these number of Gaussians +- All models are combined into a single result. diff --git a/workflows/scRNAseq/baredsc/baredSC-1d-logNorm-tests.yml b/workflows/scRNAseq/baredsc/baredSC-1d-logNorm-tests.yml new file mode 100644 index 000000000..4e1a1210a --- /dev/null +++ b/workflows/scRNAseq/baredsc/baredSC-1d-logNorm-tests.yml @@ -0,0 +1,174 @@ +- doc: Test outline for baredSC_1d + job: + Tabular with raw expression values: + class: File + path: test-data/nih3t3_generated_2d_2.txt + filetype: tabular + Gene name: 0.5_0_0_0.5_x + Maximum value in logNorm: '2.5' + Maximum number of Gaussians to study: '4' + outputs: + baredsc_numpy: + element_tests: + split_file_000000.tabular: + asserts: + has_size: + value: 1257919 + delta: 100000 + split_file_000001.tabular: + asserts: + has_size: + value: 1601519 + delta: 100000 + split_file_000002.tabular: + asserts: + has_size: + value: 2180423 + delta: 200000 + split_file_000003.tabular: + asserts: + has_size: + value: 28234812 + delta: 2000000 + baredsc_qc_plots: + element_tests: + split_file_000000.tabular: + element_tests: + convergence: + asserts: + has_size: + value: 20304 + delta: 1000 + corner: + asserts: + has_size: + value: 63603 + delta: 6000 + p: + asserts: + has_size: + value: 45302 + delta: 4000 + split_file_000001.tabular: + element_tests: + convergence: + asserts: + has_size: + value: 27704 + delta: 2000 + corner: + asserts: + has_size: + value: 387365 + delta: 30000 + p: + asserts: + has_size: + value: 97623 + delta: 90000 + split_file_000002.tabular: + element_tests: + convergence: + asserts: + has_size: + value: 32463 + delta: 3000 + corner: + asserts: + has_size: + value: 1103840 + delta: 100000 + p: + asserts: + has_size: + value: 159797 + delta: 10000 + split_file_000003.tabular: + element_tests: + convergence: + asserts: + has_size: + value: 31343 + delta: 3000 + corner: + asserts: + has_size: + value: 2139411 + delta: 200000 + p: + asserts: + has_size: + value: 175774 + delta: 10000 + baredsc_neff: + element_tests: + split_file_000000.tabular: + asserts: + has_n_lines: + n: 1 + has_line_matching: + expression: "80[0-9][0-9].[0-9]*" + split_file_000001.tabular: + asserts: + has_n_lines: + n: 1 + has_line_matching: + expression: "13[0-9][0-9].[0-9]*" + split_file_000002.tabular: + asserts: + has_n_lines: + n: 1 + has_line_matching: + expression: "2[0-9][0-9].[0-9]*" + split_file_000003.tabular: + asserts: + has_n_lines: + n: 1 + has_line_matching: + expression: "[7-9][0-9][0-9].[0-9]*" + combined_other_outputs: + element_tests: + individuals: + asserts: + has_size: + value: 108407 + delta: 10000 + means: + asserts: + has_n_lines: + n: 99998 + delta: 4000 + has_line_matching: + expression: "6.[0-9]*e-01" + posterior_andco: + asserts: + has_size: + value: 197980 + delta: 10000 + posterior_individuals: + asserts: + has_size: + value: 105262 + delta: 10000 + posterior_per_cell: + asserts: + has_n_lines: + n: 2362 + has_line_matching: + expression: "mu\tsd" + with_posterior: + asserts: + has_size: + value: 234303 + delta: 20000 + combined_pdf: + asserts: + has_line: + line: "x\tlow\tmean\thigh\tmedian" + has_text: + text: "0.0125\t" + combined_plot: + path: test-data/combined_1d_plot.png + compare: sim_size + delta_frac: 0.1 + \ No newline at end of file diff --git a/workflows/scRNAseq/baredsc/baredSC-1d-logNorm.ga b/workflows/scRNAseq/baredsc/baredSC-1d-logNorm.ga new file mode 100644 index 000000000..8f6b0990e --- /dev/null +++ b/workflows/scRNAseq/baredsc/baredSC-1d-logNorm.ga @@ -0,0 +1,636 @@ +{ + "a_galaxy_workflow": "true", + "annotation": "Run baredSC in 1 dimension in logNorm for 1 to N gaussians and combine models.", + "creator": [ + { + "class": "Person", + "identifier": "https://orcid.org/0000-0002-1964-4960", + "name": "Lucille Delisle" + } + ], + "format-version": "0.1", + "license": "MIT", + "release": "0.4", + "name": "baredSC_1d_logNorm", + "steps": { + "0": { + "annotation": "The dataset must have a first row with row names. 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+ "uuid": "1ea0b92d-d863-435a-96ac-1e547fdbc994", + "version": 7 +} \ No newline at end of file diff --git a/workflows/scRNAseq/baredsc/baredSC-2d-logNorm-tests.yml b/workflows/scRNAseq/baredsc/baredSC-2d-logNorm-tests.yml new file mode 100644 index 000000000..7f5a7bd9a --- /dev/null +++ b/workflows/scRNAseq/baredsc/baredSC-2d-logNorm-tests.yml @@ -0,0 +1,171 @@ +- doc: Test outline for baredSC_2d + job: + Tabular with raw expression values: + class: File + path: test-data/nih3t3_generated_2d_2.txt + filetype: tabular + Gene name for x axis: 0.5_0_0_0.5_x + Gene name for y axis: 0.5_0_0_0.5_y + maximum value in logNorm for x-axis: '2.5' + maximum value in logNorm for y-axis: '2.5' + Maximum number of Gaussians to study: '4' + compute p-value: 'true' + outputs: + baredsc_numpy: + element_tests: + split_file_000000.tabular: + asserts: + has_size: + value: 1654994 + delta: 100000 + split_file_000001.tabular: + asserts: + has_size: + value: 2650966 + delta: 200000 + split_file_000002.tabular: + asserts: + has_size: + value: 39060170 + delta: 3000000 + split_file_000003.tabular: + asserts: + has_size: + value: 49913268 + delta: 5000000 + baredsc_qc_plots: + element_tests: + split_file_000000.tabular: + element_tests: + convergence: + asserts: + has_size: + value: 24300 + delta: 2000 + corner: + asserts: + has_size: + value: 300500 + delta: 30000 + p: + asserts: + has_size: + value: 98200 + delta: 10000 + split_file_000001.tabular: + element_tests: + convergence: + convergence: + asserts: + has_size: + value: 28600 + delta: 2000 + corner: + asserts: + has_size: + value: 2000000 + delta: 200000 + p: + asserts: + has_size: + value: 203100 + delta: 20000 + split_file_000002.tabular: + element_tests: + convergence: + asserts: + has_size: + value: 30200 + delta: 3000 + corner: + asserts: + has_size: + value: 5100000 + delta: 500000 + p: + asserts: + has_size: + value: 269500 + delta: 20000 + split_file_000003.tabular: + element_tests: + convergence: + asserts: + has_size: + value: 31500 + delta: 3000 + corner: + asserts: + has_size: + value: 9400000 + delta: 900000 + p: + asserts: + has_size: + value: 373300 + delta: 30000 + baredsc_neff: + element_tests: + split_file_000000.tabular: + asserts: + has_n_lines: + n: 1 + has_line_matching: + expression: "[1-2]?[0-9][0-9][0-9].[0-9]*" + split_file_000001.tabular: + asserts: + has_n_lines: + n: 1 + has_line_matching: + expression: "[1-2]?[0-9][0-9][0-9].[0-9]*" + split_file_000002.tabular: + asserts: + has_n_lines: + n: 1 + has_line_matching: + expression: "[1-2]?[0-9][0-9][0-9].[0-9]*" + split_file_000003.tabular: + asserts: + has_n_lines: + n: 1 + has_line_matching: + expression: "[1-2]?[0-9][0-9][0-9].[0-9]*" + combined_other_outputs: + element_tests: + corr: + asserts: + has_n_lines: + n: 2 + has_text_matching: + expression: "0.89[0-9]*" + has_text: + text: "mean\tmedian\tlow\thigh\tpval\terror" + individuals: + asserts: + has_size: + value: 54400 + delta: 5000 + median: + asserts: + 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0.34921474837059063 1.0488463942447201 1.0 diff --git a/workflows/scRNAseq/fastq-to-matrix-10x/.dockstore.yml b/workflows/scRNAseq/fastq-to-matrix-10x/.dockstore.yml new file mode 100644 index 000000000..a092de320 --- /dev/null +++ b/workflows/scRNAseq/fastq-to-matrix-10x/.dockstore.yml @@ -0,0 +1,36 @@ +version: 1.2 +workflows: +- name: scrna-seq-fastq-to-matrix-10x-cellplex + subclass: Galaxy + publish: true + primaryDescriptorPath: /scrna-seq-fastq-to-matrix-10x-cellplex.ga + testParameterFiles: + - /scrna-seq-fastq-to-matrix-10x-cellplex-tests.yml + authors: + - name: Lucille Delisle + orcid: 0000-0002-1964-4960 + - name: Mehmet Tekman + orcid: 0000-0002-4181-2676 + - name: Hans-Rudolf Hotz + orcid: 0000-0002-2799-424X + - name: Daniel Blankenberg + orcid: 0000-0002-6833-9049 + - name: Wendi Bacon + orcid: 0000-0002-8170-8806 +- name: scrna-seq-fastq-to-matrix-10x-v3 + subclass: Galaxy + publish: true + primaryDescriptorPath: /scrna-seq-fastq-to-matrix-10x-v3.ga + testParameterFiles: + - /scrna-seq-fastq-to-matrix-10x-v3-tests.yml + authors: + - name: Lucille Delisle + orcid: 0000-0002-1964-4960 + - name: Mehmet Tekman + orcid: 0000-0002-4181-2676 + - name: Hans-Rudolf Hotz + orcid: 0000-0002-2799-424X + - name: Daniel Blankenberg + orcid: 0000-0002-6833-9049 + - name: Wendi Bacon + orcid: 0000-0002-8170-8806 diff --git a/workflows/scRNAseq/fastq-to-matrix-10x/CHANGELOG.md b/workflows/scRNAseq/fastq-to-matrix-10x/CHANGELOG.md new file mode 100644 index 000000000..9771fe8a1 --- /dev/null +++ b/workflows/scRNAseq/fastq-to-matrix-10x/CHANGELOG.md @@ -0,0 +1,27 @@ +# Changelog + +## [0.4] 2024-04-08 + +### Automatic update +- `toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_replace_in_line/9.3+galaxy0` was updated to `toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_replace_in_line/9.3+galaxy1` +- `toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_awk_tool/9.3+galaxy0` was updated to `toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_awk_tool/9.3+galaxy1` +- `toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_replace_in_line/9.3+galaxy0` was updated to `toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_replace_in_line/9.3+galaxy1` + +## [0.3] 2024-02-12 + +### Automatic update +- `toolshed.g2.bx.psu.edu/repos/iuc/rna_starsolo/rna_starsolo/2.7.10b+galaxy4` was updated to `toolshed.g2.bx.psu.edu/repos/iuc/rna_starsolo/rna_starsolo/2.7.11a+galaxy0` +- `toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_replace_in_line/1.1.2` was updated to `toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_replace_in_line/9.3+galaxy0` +- `toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_awk_tool/1.1.2` was updated to `toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_awk_tool/9.3+galaxy0` + +## [0.2] 2024-02-05 + +### Tool updates +- `toolshed.g2.bx.psu.edu/repos/iuc/rgrnastar/rna_star/2.7.10b+galaxy3` was updated to `toolshed.g2.bx.psu.edu/repos/iuc/rgrnastar/rna_star/2.7.10b+galaxy4` +- `pick_value` was replaced by `toolshed.g2.bx.psu.edu/repos/iuc/pick_value/pick_value/0.2.0` + + + +## [0.1] 2023-12-21 + +First release. diff --git a/workflows/scRNAseq/fastq-to-matrix-10x/README.md b/workflows/scRNAseq/fastq-to-matrix-10x/README.md new file mode 100644 index 000000000..a8b4a2b57 --- /dev/null +++ b/workflows/scRNAseq/fastq-to-matrix-10x/README.md @@ -0,0 +1,55 @@ +# Single-cell RNA-seq fastq to matrix for 10X data + +These workflows are inspired by the [training material](https://training.galaxyproject.org/training-material/topics/single-cell/tutorials/scrna-preprocessing-tenx/tutorial.html). Except that the output is in a 'bundle' format: three files (one matrix, one with genes, one with barcodes) which is similar to the cellranger output format. + +Both are designed for fastqs from 10X libraries v3. One is for regular 10X library (one library per sample), while the other one is for CellPlex 10X library which allows to multiplex samples using CMOs (see [this blog article](https://www.10xgenomics.com/blog/answering-your-questions-about-sample-multiplexing-for-single-cell-gene-expression)). + +## Input datasets + +- Specific for each experiment: + - For both workflows: you need a list of pairs of fastqs with gene expression. + - For CellPlex: you need in addition a list of pairs of fastqs with CMO. + - For CellPlex: you need a list of csv which describes samples and CMO used: + - first column is the sequence and second column is the name + /!\ The order of samples need to be exactly the same between the collection of fastqs of CMO and the collection of csv. + +- Common for all experiments: + - Gene annotations: A gtf file with gene locations + - List of barcodes used by 10X. You can download it at https://zenodo.org/record/3457880/files/3M-february-2018.txt.gz + +## Input values + +- reference genome: this genome needs to be available for STAR +- Barcode Size is same size of the Read: if the length of your R1 of GEX matches the size of cell barcode + UMI set to true. If your R1 contains trailling A, put false. +- number of cells: If you make it too large no cell barcode correction will be performed to demultiplex CMOs. + +## Processing +- Gene expression processing: + - Reads are aligned to the genome, asigned to genes, cell barcode and UMI with STAR Solo + - MultiQC report the mapping rate and the number of reads attributed to genes + - The output of STAR Solo is filtered with Droplet Utils to remove cellular barcodes which are probably empty. + - The output of Droplet Utils is reorganized to be: +``` +Main Collection: + - Sample 1: + - matrix.mtx + - barcodes.tsv + - genes.tsv + - Sample 2: + - matrix.mtx + - barcodes.tsv + - genes.tsv +... +``` +For the CellPlex workflow: +- CMO processing: + - CITE-Seq Count is used to asign reads and generate a matrix where 'genes' are the CMO and 'unmapped'. + - Cellular barcodes are translated to match the cellular barcodes of Gene expression see [this article](https://kb.10xgenomics.com/hc/en-us/articles/360031133451-Why-is-there-a-discrepancy-in-the-3M-february-2018-txt-barcode-whitelist-). + - Reorganize the output with UMI matrices to match the same structure as gene expression matrices. + +## Test data + +The test dataset has been produced to make it as small as possible in order to make the workflow pass on CI. + +- The CMO reads come from [zenodo](https://zenodo.org/records/10229382) and have been sampled to 0.1 with seqtk. +- The GEX reads come from SRR13948489 but have been subsetted to the cells selected in the above zenodo. diff --git a/workflows/scRNAseq/fastq-to-matrix-10x/scrna-seq-fastq-to-matrix-10x-cellplex-tests.yml b/workflows/scRNAseq/fastq-to-matrix-10x/scrna-seq-fastq-to-matrix-10x-cellplex-tests.yml new file mode 100644 index 000000000..eb13dcea9 --- /dev/null +++ b/workflows/scRNAseq/fastq-to-matrix-10x/scrna-seq-fastq-to-matrix-10x-cellplex-tests.yml @@ -0,0 +1,112 @@ +- doc: Test outline for scrna-seq-fastq-to-matrix-10X-cellplex.ga + job: + fastq PE collection GEX: + class: Collection + collection_type: list:paired + elements: + - class: Collection + type: paired + identifier: subsample + elements: + - identifier: forward + class: File + location: https://zenodo.org/records/10412836/files/GEX_R1.fastqsanger.gz + filetype: fastqsanger.gz + - identifier: reverse + class: File + location: https://zenodo.org/records/10412836/files/GEX_R2.fastqsanger.gz + filetype: fastqsanger.gz + reference genome: dm6 # To test on usegalaxy.eu dm6full + Barcode Size is same size of the Read: false + gtf: + class: File + location: https://zenodo.org/record/6457007/files/Drosophila_melanogaster.BDGP6.32.109_UCSC.gtf.gz + decompress: true + filetype: gtf + cellranger_barcodes_3M-february-2018.txt: + class: File + path: test-data/all_cell_barcodes_both_versions.txt + filetype: tabular + fastq PE collection CMO: + class: Collection + collection_type: list:paired + elements: + - class: Collection + type: paired + identifier: subsample + elements: + - identifier: forward + class: File + location: https://zenodo.org/records/10412836/files/CMO_R1.fastqsanger.gz + filetype: fastqsanger.gz + - identifier: reverse + class: File + location: https://zenodo.org/records/10412836/files/CMO_R2.fastqsanger.gz + filetype: fastqsanger.gz + sample name and CMO sequence collection: + class: Collection + collection_type: list + elements: + - class: File + identifier: subsample + location: https://zenodo.org/records/10229382/files/CMO.csv + filetype: csv + Number of expected cells: 500 + outputs: + MultiQC_STARsolo: + class: File + asserts: + - that: "has_text_matching" + expression: ">subsample
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\"coverage_file\": false}, \"input_options\": {\"input_mode\": \"short_reads\", \"__current_case__\": 0, \"input_bam\": {\"__class__\": \"ConnectedValue\"}}, \"rna_strandness\": {\"__class__\": \"ConnectedValue\"}, \"__page__\": null, \"__rerun_remap_job_id__\": null}", + "tool_version": "2.2.1+galaxy1", "type": "tool", - "uuid": "852c1da8-6826-4a58-afc7-7eb26b668f00", + "uuid": "4c7d6852-faac-4269-a7fa-92f93ebdab4c", + "when": "$(inputs.when)", "workflow_outputs": [ { - "label": "negative strand coverage", - "output_name": "out_file1", - "uuid": "1fbf821a-ff58-4c2a-b4be-386bf19b8bc8" + "label": "genes_expression_stringtie", + "output_name": "gene_abundance_estimation", + "uuid": "bd391675-83ab-4eac-8083-be71c69b786f" } ] } @@ -1258,6 +1825,6 @@ "tags": [ "RNAseq" ], - "uuid": "5b946edc-98aa-43d9-b416-978e6e8053f4", - "version": 1 + "uuid": "065bd049-36f9-4ff9-95ea-effca41a691e", + "version": 2 } \ No newline at end of file diff --git a/workflows/transcriptomics/rnaseq-sr/.dockstore.yml b/workflows/transcriptomics/rnaseq-sr/.dockstore.yml index 4b542ec01..61f298b5c 100644 --- a/workflows/transcriptomics/rnaseq-sr/.dockstore.yml +++ b/workflows/transcriptomics/rnaseq-sr/.dockstore.yml @@ -1,7 +1,11 @@ version: 1.2 workflows: - name: main - primaryDescriptorPath: /rnaseq-sr.ga subclass: Galaxy + publish: true + primaryDescriptorPath: /rnaseq-sr.ga testParameterFiles: - /rnaseq-sr-tests.yml + authors: + - name: Lucille Delisle + orcid: 0000-0002-1964-4960 diff --git a/workflows/transcriptomics/rnaseq-sr/CHANGELOG.md b/workflows/transcriptomics/rnaseq-sr/CHANGELOG.md index c5362e5be..a819c76d8 100644 --- a/workflows/transcriptomics/rnaseq-sr/CHANGELOG.md +++ b/workflows/transcriptomics/rnaseq-sr/CHANGELOG.md @@ -1,5 +1,42 @@ # Changelog +## [0.6] 2024-02-05 + +### Automatic update +- `toolshed.g2.bx.psu.edu/repos/lparsons/cutadapt/cutadapt/4.4+galaxy0` was updated to `toolshed.g2.bx.psu.edu/repos/lparsons/cutadapt/cutadapt/4.6+galaxy1` +- `toolshed.g2.bx.psu.edu/repos/iuc/map_param_value/map_param_value/0.1.1` was updated to `toolshed.g2.bx.psu.edu/repos/iuc/map_param_value/map_param_value/0.2.0` +- `toolshed.g2.bx.psu.edu/repos/iuc/rgrnastar/rna_star/2.7.10b+galaxy4` was updated to `toolshed.g2.bx.psu.edu/repos/iuc/rgrnastar/rna_star/2.7.11a+galaxy0` +- `toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_awk_tool/1.1.2` was updated to `toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_awk_tool/9.3+galaxy0` +- `toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_find_and_replace/1.1.4` was updated to `toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_find_and_replace/9.3+galaxy0` + +## [0.5] 2023-09-15 + +### Automatic update +- `toolshed.g2.bx.psu.edu/repos/iuc/rgrnastar/rna_star/2.7.8a+galaxy1` was updated to `toolshed.g2.bx.psu.edu/repos/iuc/rgrnastar/rna_star/2.7.10b+galaxy4` + +### Manual update +- Use STAR to compute normalized strand-specific coverage +- Add an option to use StringTie to compute FPKM +- Make cufflinks step optional + +## [0.4.1] 2023-09-14 +- add author in dockstore file + +## [0.4] 2023-01-16 + +### Automatic update +- `toolshed.g2.bx.psu.edu/repos/devteam/bamtools_filter/bamFilter/2.5.1+galaxy0` was updated to `toolshed.g2.bx.psu.edu/repos/devteam/bamtools_filter/bamFilter/2.5.2+galaxy1` + +## [0.3] 2022-12-17 + +### Automatic update +- `toolshed.g2.bx.psu.edu/repos/iuc/multiqc/multiqc/1.11+galaxy0` was updated to `toolshed.g2.bx.psu.edu/repos/iuc/multiqc/multiqc/1.11+galaxy1` + +## [0.2] 2022-11-02 + +### Automatic update +- `toolshed.g2.bx.psu.edu/repos/iuc/rgrnastar/rna_star/2.7.8a+galaxy0` was updated to `toolshed.g2.bx.psu.edu/repos/iuc/rgrnastar/rna_star/2.7.8a+galaxy1` + ## [0.1] 2022-10-12 First release. diff --git a/workflows/transcriptomics/rnaseq-sr/README.md b/workflows/transcriptomics/rnaseq-sr/README.md index 7340d24e5..09236c628 100644 --- a/workflows/transcriptomics/rnaseq-sr/README.md +++ b/workflows/transcriptomics/rnaseq-sr/README.md @@ -2,7 +2,7 @@ ## Inputs dataset -- The workflow needs a list of dataset of fastqsanger. +- The workflow needs a list of datasets of fastqsanger. - As well as a gtf file with genes - Optional, but recommended: a gtf file with regions to exclude from normalization in Cufflinks. @@ -15,26 +15,29 @@ chrM chrM_gene exon 0 16299 . - . gene_id "chrM_gene_minus"; transcript_id "chrM ## Inputs values -- forward adapter sequence: this depends on the library preparation. Usually classical RNA libraries are Truseq and ISML (relatively new Illumina library) is Nextera. If you don't know, use FastQC to determine if it is Truseq or Nextera. If the read length is relatively short (50bp), there is probably no adapter. +- forward adapter sequence: this depends on the library preparation. Usually classical Illumina RNA libraries are Truseq and ISML (relatively new Illumina library) is Nextera. If you don't know, use FastQC to determine if it is Truseq or Nextera. If the read length is relatively short (50bp), there is probably no adapter so it will not impact your results. - reference_genome: this field will be adapted to the genomes available for STAR -- strandness: For stranded RNA, reverse means that the read is complementary to the coding sequence, forward means that the read is in the same orientation as the coding sequence. This will help you to get from STAR only the counts corresponding to your library preparation. This is also used for the stranded coverage and for FPKM computation with cufflinks. +- strandedness: For stranded RNA, reverse means that the read is complementary to the coding sequence, forward means that the read is in the same orientation as the coding sequence. This will only count alignments that are compatible with your library preparation strategy. This is also used for the stranded coverage and for FPKM computation with cufflinks/StringTie. +- cufflinks_FPKM: Whether you want to get FPKM with Cufflinks (pretty long) +- stringtie_FPKM: Whether you want to get FPKM/TPM etc... with Stringtie. ## Processing -- The workflow will remove adapters and low quality bases and filter out any read smaller than 15bp -- The filtered reads are mapped with STAR with ENCODE parameters (for long RNA-seq but I use it for short also). STAR is also used to count reads per gene. -- A multiQC is run to have an overview of the QC. This can also be used to get the strandness. -- FPKM values for reads and transcripts are computed with cufflinks using correction for multi-mapped reads. +- The workflow will remove adapters and low quality bases and filter out any read smaller than 15bp. +- The filtered reads are mapped with STAR with ENCODE parameters (for long RNA-seq but I use it for short also). STAR is also used to count reads per gene and generate strand-specific normalized coverage (on uniquely mapped reads). +- A multiQC is run to have an overview of the QC. This can also be used to get the strandedness. +- FPKM values for genes and transcripts are computed with cufflinks using correction for multi-mapped reads (this step is optionnal). +- FPKM/TPM values for genes are computed with StringTie (this step is optional). - The BAM is filtered to keep only uniquely mapped reads (tag NH:i:1). -- Coverage unstranded, and each strand independently is computed with bedtools and normalized to the number of million uniquely mapped reads. +- Unstranded coverage is computed with bedtools and normalized to the number of million uniquely mapped reads. - The three coverage files are converted to bigwig. ### Warning -- The coverage stranded output depends on the strandness of the library: +- The coverage stranded output depends on the strandedness of the library: - If you have an unstranded library, stranded coverages are useless - If you have a forward stranded library, the label matches the orientation of reads. - - If you have a reverse stranded library, `positive strand coverage` should correspond to genes on the forward strand and uses the reads mapped on the reverse strand. `negative strand coverage` should correspond to genes on the reverse strand and uses the reads mapped on the forward strand. + - If you have a reverse stranded library, `forward` should correspond to genes on the forward strand and uses the reads mapped on the reverse strand. `reverse` should correspond to genes on the reverse strand and uses the reads mapped on the forward strand. ## Contribution diff --git a/workflows/transcriptomics/rnaseq-sr/rnaseq-sr-tests.yml b/workflows/transcriptomics/rnaseq-sr/rnaseq-sr-tests.yml index 86eefc13d..14e27ea2f 100644 --- a/workflows/transcriptomics/rnaseq-sr/rnaseq-sr-tests.yml +++ b/workflows/transcriptomics/rnaseq-sr/rnaseq-sr-tests.yml @@ -13,7 +13,9 @@ location: https://zenodo.org/record/4541751/files/GSM461177_1_subsampled.fastqsanger forward_adapter: GATCGGAAGAGCACACGTCTGAACTCCAGTCAC reference_genome: dm6 - strandness: unstranded + strandedness: unstranded + cufflinks_FPKM: true + stringtie_FPKM: true outputs: output_log: element_tests: @@ -24,7 +26,7 @@ - that: "has_text" text: "Uniquely mapped reads number |\t871202" - that: "has_text" - text: "Number of reads mapped to multiple loci |\t91809" + text: "Number of reads mapped to multiple loci |\t91808" mapped-reads: element_tests: GSM461177: @@ -37,7 +39,7 @@ cutadapt: asserts: has_text: - text: "GSM461177\t4.0\t1057657\t25033\t6191\t1051466\t39133309\t1439589\t37547108\t4.053327051898423" + text: "GSM461177\t4.6\t1057657\t25033\t6191\t1051466\t39133309\t1439589\t37547108\t4.053327051898423" general_stats: asserts: has_text: @@ -48,8 +50,8 @@ n: 4 star: asserts: - has_text: - text: "GSM461177\t1051466.0\t35.0\t871202.0\t82.86\t35.4\t51184.0\t50995.0\t50810.0\t343.0\t12.0\t19.0\t0.48\t0.0\t1.48\t0.0\t1.36\t91809.0\t8.73\t34515.0\t3.28\t0.0\t4.76\t0.37\t0\t50050\t3890" + has_text_matching: + expression: "GSM461177\t1051466.0\t35.0\t871202.0\t82.86\t35.4\t51184.0\t50995.0\t50810.0\t343.0\t12.0\t19.0\t0.48\t0.0\t1.48\t0.0\t1.36\t9180[89].0\t8.73\t34515.0\t3.28\t0.0\t4.76\t0.37\t0\t5005[01]\t3890" MultiQC webpage: asserts: - that: "has_text" @@ -72,33 +74,37 @@ asserts: has_text: text: "FBgn0010247\t14" - transcripts_expression: + transcripts_expression_cufflinks: element_tests: GSM461177: asserts: has_text: - text: "FBtr0078104\t-\t-\tFBgn0031217\tCG11377\t-\tchr2L:102379-104142\t1583\t0.626702\t18.291\t9.78604\t26.796\tOK" - genes_expression: + text: "FBtr0078104\t-\t-\tFBgn0031217\tCG11377\t-\tchr2L:102379-104142\t1583\t0.626702\t18.291\t9.78605\t26.796\tOK" + genes_expression_cufflinks: + element_tests: + GSM461177: + asserts: + has_text_matching: + expression: "FBgn0031217\t-\t-\tFBgn0031217\tCG11377\t-\tchr2L:102379-104142\t-\t-\t32.1016\t22.1771\t42.02[0-9]*\tOK" + genes_expression_stringtie: element_tests: GSM461177: asserts: has_text: - text: "FBgn0031217\t-\t-\tFBgn0031217\tCG11377\t-\tchr2L:102379-104142\t-\t-\t32.1015\t22.1771\t42.0259\tOK" + text: "FBgn0031217\tCG11377\tchr2L\t+\t102380\t104142\t0.994315\t32.391769\t56.255165" both strands coverage: element_tests: GSM461177: has_size: value: 6075761 delta: 600000 - negative strand coverage: + stranded coverage: element_tests: - GSM461177: + GSM461177_reverse: has_size: value: 3103918 delta: 300000 - positive strand coverage: - element_tests: - GSM461177: + GSM461177_forward: has_size: value: 3103918 delta: 300000 diff --git a/workflows/transcriptomics/rnaseq-sr/rnaseq-sr.ga b/workflows/transcriptomics/rnaseq-sr/rnaseq-sr.ga index 356f3adc3..c214939d5 100644 --- a/workflows/transcriptomics/rnaseq-sr/rnaseq-sr.ga +++ b/workflows/transcriptomics/rnaseq-sr/rnaseq-sr.ga @@ -1,6 +1,6 @@ { "a_galaxy_workflow": "true", - "annotation": "This workflow takes as input a list of single-read fastqs. Adapters and bad quality bases are removed with cutadapt. Reads are mapped with STAR with ENCODE parameters and genes are counted simultaneously. The counts are reprocess to be similar to HTSeq-count output. FPKM are computed with cufflinks. Coverage (per million mapped reads) are computed with bedtools on uniquely mapped reads.", + "annotation": "This workflow takes as input a list of single-reads fastqs. Adapters and bad quality bases are removed with cutadapt. Reads are mapped with STAR with ENCODE parameters and genes are counted simultaneously as well as normalized coverage (per million mapped reads) on uniquely mapped reads. The counts are reprocessed to be similar to HTSeq-count output. FPKM are computed with cufflinks and/or with StringTie. The unstranded normalized coverage is computed with bedtools.", "creator": [ { "class": "Person", @@ -11,7 +11,7 @@ "format-version": "0.1", "license": "MIT", "name": "RNAseq_SR", - "release": "0.1", + "release": "0.6", "steps": { "0": { "annotation": "Should be a list of single-read RNA-seq fastqs", @@ -29,14 +29,15 @@ "name": "Input dataset collection", "outputs": [], "position": { - "left": 0.0, - "top": 212.8770301624129 + "left": 0, + "top": 354 }, "tool_id": null, - "tool_state": "{\"optional\": false, \"tag\": \"\", \"collection_type\": \"list\"}", + "tool_state": "{\"optional\": false, \"tag\": null, \"collection_type\": \"list\"}", "tool_version": null, "type": "data_collection_input", - "uuid": "688da967-e739-409a-b950-b4e4985a1a3b", + "uuid": "633ddb66-8a2b-4822-b894-2e947bf6607b", + "when": null, "workflow_outputs": [] }, "1": { @@ -55,14 +56,15 @@ "name": "Input parameter", "outputs": [], "position": { - "left": 43.890171980359995, - "top": 285.8662262316923 + "left": 42.45001220703125, + "top": 430.91668701171875 }, "tool_id": null, "tool_state": "{\"parameter_type\": \"text\", \"optional\": false}", "tool_version": null, "type": "parameter_input", "uuid": "0179b655-f7e2-4b8b-b8b1-f875d0d8039f", + "when": null, "workflow_outputs": [] }, "2": { @@ -81,14 +83,15 @@ "name": "Input parameter", "outputs": [], "position": { - "left": 85.96286640919556, - "top": 377.90023201856144 + "left": 107.63336181640625, + "top": 522.134886401586 }, "tool_id": null, "tool_state": "{\"restrictOnConnections\": true, \"parameter_type\": \"text\", \"optional\": false}", "tool_version": null, "type": "parameter_input", "uuid": "ff4a9932-6ed0-436f-a63c-d6fbe2a582a3", + "when": null, "workflow_outputs": [] }, "3": { @@ -107,14 +110,15 @@ "name": "Input dataset", "outputs": [], "position": { - "left": 123.78189901188189, - "top": 469.9149430489595 + "left": 125.60003662109375, + "top": 606.751585620336 }, "tool_id": null, "tool_state": "{\"optional\": false, \"tag\": \"\"}", "tool_version": null, "type": "data_input", "uuid": "c91756d4-f7f1-4dcc-97f6-da4c96a53c94", + "when": null, "workflow_outputs": [] }, "4": { @@ -126,54 +130,110 @@ "inputs": [ { "description": "For stranded RNA, reverse means that the read is complementary to the coding sequence, forward means that the read is in the same orientation as the coding sequence", - "name": "strandness" + "name": "strandedness" } ], - "label": "strandness", + "label": "strandedness", "name": "Input parameter", "outputs": [], "position": { - "left": 170.8429960529655, - "top": 554.9304192138105 + "left": 156.25, + "top": 667.118284839086 }, "tool_id": null, "tool_state": "{\"restrictions\": [\"stranded - forward\", \"stranded - reverse\", \"unstranded\"], \"parameter_type\": \"text\", \"optional\": false}", "tool_version": null, "type": "parameter_input", "uuid": "fd2c51fd-58b3-43b5-b8ce-a919f5325cb7", + "when": null, "workflow_outputs": [] }, "5": { - "annotation": "Could be a gtf with for example one entry for the chrM forward and one entry for the chrM reverse", + "annotation": "Whether FPKM values should be computed with cufflinks", "content_id": null, "errors": null, "id": 5, "input_connections": {}, + "inputs": [ + { + "description": "Whether FPKM values should be computed with cufflinks", + "name": "cufflinks_FPKM" + } + ], + "label": "cufflinks_FPKM", + "name": "Input parameter", + "outputs": [], + "position": { + "left": 177.6500244140625, + "top": 735.4800895070166 + }, + "tool_id": null, + "tool_state": "{\"parameter_type\": \"boolean\", \"optional\": false}", + "tool_version": null, + "type": "parameter_input", + "uuid": "fbe4c961-6b62-46b7-a329-163b9cda4979", + "when": null, + "workflow_outputs": [] + }, + "6": { + "annotation": "Could be a gtf with for example one entry for the chrM forward and one entry for the chrM reverse", + "content_id": null, + "errors": null, + "id": 6, + "input_connections": {}, "inputs": [ { "description": "Could be a gtf with for example one entry for the chrM forward and one entry for the chrM reverse", - "name": "gtf with regions to exclude from FPKM normalization" + "name": "gtf with regions to exclude from FPKM normalization with Cufflinks" } ], - "label": "gtf with regions to exclude from FPKM normalization", + "label": "gtf with regions to exclude from FPKM normalization with Cufflinks", "name": "Input dataset", "outputs": [], "position": { - "left": 236.91027789547383, - "top": 658.8167159573937 + "left": 201.73333740234375, + "top": 809.5967887257666 }, "tool_id": null, "tool_state": "{\"optional\": true, \"tag\": \"\"}", "tool_version": null, "type": "data_input", "uuid": "cc52d397-17a9-4c38-85f6-a7c0b8f21985", + "when": null, "workflow_outputs": [] }, - "6": { + "7": { + "annotation": "Whether FPKM values should be computed with StringTie", + "content_id": null, + "errors": null, + "id": 7, + "input_connections": {}, + "inputs": [ + { + "description": "Whether FPKM values should be computed with StringTie", + "name": "stringtie_FPKM" + } + ], + "label": "stringtie_FPKM", + "name": "Input parameter", + "outputs": [], + "position": { + "left": 245.88330078125, + "top": 926.4134391163916 + }, + "tool_id": null, + "tool_state": "{\"parameter_type\": \"boolean\", \"optional\": false}", + "tool_version": null, + "type": "parameter_input", + "uuid": "9619cb07-6835-4f79-8f49-ce59e343736d", + "when": null, + "workflow_outputs": [] + }, + "8": { "annotation": "", - "content_id": "toolshed.g2.bx.psu.edu/repos/lparsons/cutadapt/cutadapt/4.0+galaxy1", + "content_id": "toolshed.g2.bx.psu.edu/repos/lparsons/cutadapt/cutadapt/4.6+galaxy1", "errors": null, - "id": 6, + "id": 8, "input_connections": { "library|input_1": { "id": 0, @@ -198,8 +258,8 @@ } ], "position": { - "left": 318.9481837013758, - "top": 180.68445475638043 + "left": 500.61669921875, + "top": 339.134886401586 }, "post_job_actions": { "HideDatasetActionout1": { @@ -207,30 +267,36 @@ "action_type": "HideDatasetAction", "output_name": "out1" 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"genes_expression_stringtie", + "output_name": "gene_abundance_estimation", + "uuid": "bd391675-83ab-4eac-8083-be71c69b786f" } ] } @@ -1192,6 +1795,6 @@ "tags": [ "RNAseq" ], - "uuid": "49f297ff-5c0b-4efb-b696-ae25868f668a", + "uuid": "847e4ce1-5522-499c-ab7d-9a3efe06a7be", "version": 2 } \ No newline at end of file diff --git a/workflows/variant-calling/generic-variant-calling-wgs-pe/.dockstore.yml b/workflows/variant-calling/generic-variant-calling-wgs-pe/.dockstore.yml index eef6f0cf1..29408dbd3 100644 --- a/workflows/variant-calling/generic-variant-calling-wgs-pe/.dockstore.yml +++ b/workflows/variant-calling/generic-variant-calling-wgs-pe/.dockstore.yml @@ -1,10 +1,11 @@ version: 1.2 workflows: - name: main - primaryDescriptorPath: /Generic-variation-analysis-on-WGS-PE-data.ga subclass: Galaxy + publish: true + primaryDescriptorPath: /Generic-variation-analysis-on-WGS-PE-data.ga testParameterFiles: - /Generic-variation-analysis-on-WGS-PE-data-tests.yml authors: - - orcid: 0000-0002-5987-8032 - publish: true + - name: Anton Nekrutenko + orcid: 0000-0002-5987-8032 diff --git a/workflows/variant-calling/generic-variant-calling-wgs-pe/CHANGELOG.md b/workflows/variant-calling/generic-variant-calling-wgs-pe/CHANGELOG.md index 52e5a736c..e76df1cd1 100644 --- a/workflows/variant-calling/generic-variant-calling-wgs-pe/CHANGELOG.md +++ b/workflows/variant-calling/generic-variant-calling-wgs-pe/CHANGELOG.md @@ -1,5 +1,10 @@ # Changelog + +## [0.1.1] 2023-11-20 + +- Fix author in dockstore + ## [0.1] - Initial version of Generic variation analysis on WGS PE data workflow diff --git a/workflows/variant-calling/generic-variant-calling-wgs-pe/Generic-variation-analysis-on-WGS-PE-data.ga b/workflows/variant-calling/generic-variant-calling-wgs-pe/Generic-variation-analysis-on-WGS-PE-data.ga index 174d84db0..150a82224 100644 --- a/workflows/variant-calling/generic-variant-calling-wgs-pe/Generic-variation-analysis-on-WGS-PE-data.ga +++ b/workflows/variant-calling/generic-variant-calling-wgs-pe/Generic-variation-analysis-on-WGS-PE-data.ga @@ -9,7 +9,7 @@ } ], "format-version": "0.1", - "release": "0.1", + "release": "0.1.1", "license": "MIT", "name": "Generic variation analysis on WGS PE data", "steps": { diff --git a/workflows/variant-calling/variation-reporting/.dockstore.yml b/workflows/variant-calling/variation-reporting/.dockstore.yml index 578af1f19..3f4b9aa2a 100644 --- a/workflows/variant-calling/variation-reporting/.dockstore.yml +++ b/workflows/variant-calling/variation-reporting/.dockstore.yml @@ -1,8 +1,11 @@ version: 1.2 workflows: - name: main - primaryDescriptorPath: /Generic-variation-analysis-reporting.ga subclass: Galaxy + publish: true + primaryDescriptorPath: /Generic-variation-analysis-reporting.ga testParameterFiles: - /Generic-variation-analysis-reporting-tests.yml - publish: true + authors: + - name: Wolfgang Maier + orcid: 0000-0002-9464-6640 diff --git a/workflows/variant-calling/variation-reporting/CHANGELOG.md b/workflows/variant-calling/variation-reporting/CHANGELOG.md index 8e561998b..46bfcc79c 100644 --- a/workflows/variant-calling/variation-reporting/CHANGELOG.md +++ b/workflows/variant-calling/variation-reporting/CHANGELOG.md @@ -1,5 +1,9 @@ # Changelog +## [0.1.1] 2023-11-20 + +- Fix author in dockstore + ## [0.1] - Initial version generic variation analysis reporting workflow diff --git a/workflows/variant-calling/variation-reporting/Generic-variation-analysis-reporting.ga b/workflows/variant-calling/variation-reporting/Generic-variation-analysis-reporting.ga index b847e0a9c..a3a763a4e 100644 --- a/workflows/variant-calling/variation-reporting/Generic-variation-analysis-reporting.ga +++ b/workflows/variant-calling/variation-reporting/Generic-variation-analysis-reporting.ga @@ -8,8 +8,8 @@ "name": "Wolfgang Maier" } ], - "release": "0.1", "format-version": "0.1", + "release": "0.1.1", "license": "MIT", "name": "Generic variation analysis reporting", "steps": { diff --git a/workflows/virology/pox-virus-amplicon/.dockstore.yml b/workflows/virology/pox-virus-amplicon/.dockstore.yml new file mode 100644 index 000000000..4edeca8a3 --- /dev/null +++ b/workflows/virology/pox-virus-amplicon/.dockstore.yml @@ -0,0 +1,11 @@ +version: 1.2 +workflows: +- name: main + subclass: Galaxy + publish: true + primaryDescriptorPath: /pox-virus-half-genome.ga + authors: + - name: Viktoria Isabel Schwarz + orcid: 0000-0001-6897-1215 + - name: Wolfgang Maier + orcid: 0000-0002-9464-6640 diff --git a/workflows/virology/pox-virus-amplicon/CHANGELOG.md b/workflows/virology/pox-virus-amplicon/CHANGELOG.md new file mode 100644 index 000000000..f16a9be6c --- /dev/null +++ b/workflows/virology/pox-virus-amplicon/CHANGELOG.md @@ -0,0 +1,5 @@ +# Changelog + +## [0.1] + +Initial version of Pox Virus Illumina Amplicon workflow (iVar based) for half-genomes sequencing data \ No newline at end of file diff --git a/workflows/virology/pox-virus-amplicon/README.md b/workflows/virology/pox-virus-amplicon/README.md new file mode 100644 index 000000000..2bf8d7cf1 --- /dev/null +++ b/workflows/virology/pox-virus-amplicon/README.md @@ -0,0 +1,9 @@ +# Pox Virus Illumina Amplicon Workflow for half-genomes sequencing data + +This workflow generates consensus sequences from Illumina PE-sequenced ARTIC data of pox virus samples. + +It requires that all samples have been sequenced in two halves in two separate sequencing runs, and utilizes this property to resolve the inverted terminal repeat (ITR) sequences of pox virus genomes. + +The workflow uses BWA-MEM for mapping the reads from each half-genome sequencing run to a correspondingly masked version of the reference genome, merges the resulting two read mappings, and uses iVar for primer trimming and consensus sequence generation. + +Conceptually, this workflow builds on https://github.com/iwc-workflows/sars-cov-2-pe-illumina-artic-ivar-analysis and adds the logic for the split genome mapping and merging of the results. \ No newline at end of file diff --git a/workflows/virology/pox-virus-amplicon/pox-virus-half-genome-test.yml b/workflows/virology/pox-virus-amplicon/pox-virus-half-genome-test.yml new file mode 100644 index 000000000..f6c282d6f --- /dev/null +++ b/workflows/virology/pox-virus-amplicon/pox-virus-half-genome-test.yml @@ -0,0 +1,46 @@ +- doc: Testing Pox Virus Illumina Amplicon Workflow with Lumpy Skin Disease Virus half genome sequenced data + job: + Reference FASTA: + class: File + location: "https://www.ebi.ac.uk/ena/browser/api/fasta/AF325528.1?download=true" + filetype: fasta + Primer Scheme: + class: File + path: test-data/CaPV-V1_primer_scheme.bed6 + filetype: bed + PE Reads Pool1: + class: Collection + collection_type: list:paired + elements: + - class: Collection + type: paired + identifier: 20L70 # SRR15145276 + elements: + - identifier: forward + class: File + location: 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TCAACTTTCCTAGGCGTTAATTCGTCAATACATGCTTTACTTGTTTCATTTGTCCTTATTTGATAATAATCAGTTTCGTAATTTCCAAAAACCGTAACACAAACTTTTTCTTGAGAACCAAATTCAATAGCACATCCCTTTTTATCACAAAATTGTTCATCAACTTCATATTTAATTGTATTTGAATCACCAAACGTAATACTTAAAATATATTTAAACACAATATTACAATACTTATCGTTATTATATATAGGAGAATTTTTATTATTATCCTTGGCTAGTGGGTGATTAATCGTTAGTACAGTATTAACATCTTGTCGTTTAATAAACACAGATGGAGTAGTAATAACAACACTTTCACAAATTGGTTTAAACGTAAAAACGTTCGATTCTGTATTATCGACTGTAATAAATTTTATCCAAAAATCGTCAGTATTATCATCTATAAAAGGTGAAACATTACAATATTTTTGAATAGTATAATTACATGCTTGTTTCCATTCTCCACTGCTATAAGTCATAATCATTACCCTATAGTTACTATCGCTGACATCGTTAGTCCACTCGATTTTAGAATTAAAATCATTAGATGTTAGTTTTATATCTTTAGGAAAAGAATAACATAGTTTAAAGAGCAAAAAAGCAAAAAATACTACTGCGCCCATTTTTTAGTAAGTATTTAGTTATATTTTATATTTTTTATGGAAATCTATCACAGATATATTTTTCAAATTCTTTTCTCTTAAAATGACTTATTTCGTCAAAAAATATGTAAAAGCTTGGATACTTACATGTATCATGTGAAGAAATTATTGATATTAAACCTATTATATATTTCATTGTGGTTATGTCAATCACATTATTTTCATTTTTTTCATAAAGCGAAAAACAGTAACGTGTTTTACTTAAACGCAATTTTGATATTATTTTATTAATATATTCAACACCGTAGTGATTAACGGCCATAGCATCATATGTTAAAAACTCAGCATATATTTTACCTATTTTTTTTGTATTTTTCATTTTTCGTACCATAAAATAATTTTCCATCATATCGTTAAAATTTACTTTTTTCATGGAATTAATATGCTTAAAACTTTTAGATTTTATAAATGGATAAAAATCAGCATAGTATTCACCATCAATTTTTAATTCTTTTATAACTTTTTTATAATCATTTTCATTAAGTACATATTCTGATATAACTTTTATCATAGTAAACGTAAATTTATTTGAATTTTTTAATACAATCTTCGTTATGTCATCCTTCGTATAATCATAATTTGAAAGATAAAAAACTTTATTTAAAAAATATTTTACCTTTTTTTCATTGATAGAAAAAAGAAAGGTTATATACTTAGTAAAGATTTTAAATTTATTTTTTGTATACATAATGCATAACGATTAACTATTTTTAAAAAGTGTAATATTTTTTTTCATTTTTATATATACTCATAAACCTGATATGTTGTGTTATCACTCTTCGCAGCAAGATAATAATCGTACAATGCTATTAGTATATGTACTACAGTTATAAATCCCACAACACAAGGAAGGGAATATGCCATCGCGGATATTAGTGCAATAAGATCACGATTTTCAATATTTTCTAACATACTTTTCAAATTAAACTTAACTAAAAGTATGGTTAGTGGTAAAAGTAGTAAACATATTAAAGAAACGCAAATAGCTGGCACGTTACAATACATAAACGAACATTTCCATCTTAAACAACTCTTTTTATTTTTTTTAATATTGTATTTTCCATTACATATTTTACATTTTGTATTATGTGAAAAATTAATCCATTCTTCTAAACAGTTTTTATGAACTATCTTAAATTCATTCTTGCAATTACAAAAGTTTGTACTTACATTATATTCATCTTTACAAATCCAACAATGAGTGTTGGTATTATCACTCCCTTCCATTTTTATAAAATATCATTATTTGTTGTTATTATTTTTTTATTTTTTATCCAATGCTAATACTACCAGCACTACTAGTGCTACGCAATCGTAAAAGCTTTTTAGTAAATTCTCTACTACAAAACGCATAAATTAGTGGATTAATAAAACAATGACATAGAGACACAATTTCAGCTACATGAACTGCAAGGTTGACAAATCGTAATGCCGTACATCCACTAAAAACATTTAACAAATACAACGATGAAACAAATACAGTTACACTAAATGGGAGTAAAAACAATACTGAACAGATAACAATCAAAAACACCATCTTTATGGCTTTCTTATTCTTTGTTTGCGAGGTTTTTAAAGTATTTAAGATTTTATAATAACAATATAGCAAAATAGTTAGCGGTATAATCATTCCAAATATGTTTATTTCAAAATTTATAAATAATTTCCAAATTTTTGCATTATCGTTATAAAATACATGACAATACGTTATTCCATATACTTTTTTTGTTTCATAAAATAACATTATTGGAAAGGATTCAATAGTTGAGACAATCCAAACCACCATACTAAGTACAATTCCATATCGTTTTGTCCTTATCGGCATTGATTTTACTGGGTGAACTACAGCTAGGTATCTATCAATACTCATCAATGTTATAAATGACATGCTATTGTAAAAACCAACAAAGTAAAACATAGCTTTAAATTTACACAAACAATCTCCTAAACTCCATTGTTTAGCGATACTATCGTATAAATTAAAAGGAAACACCAACACGAAAATTAAATCAGACAGTGTCAAATTAAGCAAAAACATATCCTGTATTGTTTTTATCTTATATTTACGAAGAACAGTTAACACAATTATATTTCCAAATAATCCAAGAAAGAATATAGTCGAATATAAAGTAATCAGTCCAAAACTTGTAGTATCCACACCATCATCACAATGTGGGATATCGACTATGCTCACTTCATAATCATCATAATCATCGCTATAATAAGTTGTATTATATGCGGTTGTATAATTAGATATCGTTGTTGTATTTTCATAAGTTGAAGGCGTTGTAACATTATTTTGATTTGTTGAAATTGTACTGAGAATTGTGCTAATAATTGTAGTAGCTATAGTGGTAATATTACTGCTACTATTATACATGGTGGCGCTACTAACTGTGCTAAGAGTATAATTCATTTTTGTTGGAGTTGTGCTTTACTTAATCATACACTTTTATTTTATTTTTTTTTTTAATAAAAACACAGTATTATTGTATCATCATTTATTTTTTTAAAAAAACTTTTTATTGTTTGTCAAAAAAGGTGAGATTTCTAAATTTAATTAAATCCTTACAATTGTAACCTGACGTATTAGTTTCATCAATATCAATACCAAGACTAGTTAGATAATCAAATATCTTTTTATCAGAATGAAACAAAATATAACTATACAATATATTATTATTATCAAAATCTTTTTGCAAAAAATTAACTCCACTATCTATTAGTAACTTTATTACATTTATACGAACATTAAAATTACACATGTACATGTGTAACAAATTTTTCCCATTTTCATCTTCTTCTGTTACAGATGATCCAGAATCAATTAATATTTTAACTATTTCAGGTTCGACATTCTTGTTAAAAGAAAGATAATGAGCTAGTGCTGATAAATTATTATCTCTAGTTTTAAAATTTATATCGGATCCATTTTCTATGAGAAATTTTAATATTTCAACATTAACTTTATCTTTTTCGACACACGAAAACAATGGCGTTTCATATAAGTCGTTACAATCATTAACAAATTTTATCCATTTCTTTACTCCATTTATATCATCTTGTTCAACGTAATAAAATAATGGATTACAATATCTATAAAATAAGTAATCTGTAAAGTCATTATCATAATCGCTACATAATTTTTCCTTTTCCATAGTAATTGATGATGGCAAACTAGCTTATTAATAAAAAGTTATATTTCATTTTTGTAAAGTGTTTTTAAATTTACATATTTAATTTTTTTTGTGAGATAGTTGTATTCTAAGTTGCGTAAGTTACATGTGAAATTAGCAGATAGATATTTTCTTTTAACTTCTTTTATGATTAATGCAATTTTATAAGAGTTTCCTACGTACATTTCTCTACCTAAGTAAATATTTTTACTTCTATCGTAATCAAGTACATTAACTCCGTTAATTAACCAAAATATTGAACTTCCATAAACACGTTGTATGTTTTTAGATTCTCCAATACATTCCATTAAAAAGTTTTTACCTAAAGCTATTGTACTATTTAAAAAATTTACTGTTAAATTTATATCATCTTTAAATCTTTTTTTAATTACGTTATTATGATAATAACGTTTTAATGCCAAGTTTTTTGGATTACACCGTGTATCCGTAGTTATGGAACATAAATATACTCCTTCATCACTCTTAGAAATATTTCGTATTAGTAATTTTTCTTTGCTTTTTTTATATTTAAAATTATTGTTTATTTTTTCCCTGTCTTTAAACCAATTTACTTCATAAGAACAATTAAACGTATTTATAAAGTTTTTTGTATTTATACACAACAACTCATAGTTTGTGCCAATGACAGAGTTTATAGTCTCTTTTTTATAAACATCTAACATAACAGATGAAGTAAAATAATATGTTGAGTTATGTACATTACATGCATATATTCCAGAATCATTTTTTGTTATATTATTTATCCACAGGCTATGCCCAACAGAATATACTCTATCGGTATTTTTGAACTCATGAAAAGATTTTTTTACATCTCCTGTTTTAACGTATTTATTGTAATTAACTAAAAACCATTTTACTGTTGTATTATAATTTAAAGTATCAACATATGGACATTTTACTATTTCTGAGTTATTTTCGAACGTTTTCATCACGGATTGTAGACAACCAAAGCTTAATTTTGTAAAAGCAAGAATTTTAACATTGATAATCAGTAAATAGAAAATAAATGTCGTTACTTTTTCCATCTTATCCAATAAGATTTTAATCTTACAAGATTTAAAAAAAAATATTTTTTTTTTAATTTTTAGTTGTTATTATAAACTTTTACATCAATATAGCCCTTTATTTTGTTCATCCTTATAATTTGCACATTAATTAATTTATTAACTAATGTTTTTTTTATATTATTCATTTGATCCATGTTTATATTAACATAATCTATTACAAGTGCTTCCAAACCATAGTTGATTAGTTTTACATATACAATGTTATCTTTTATAAACACAATACCATCTGTTACTTCATTAATGTTAGGTAAAACGTAACAAAATGCCAAATCGCTATTCGATGACATTTTTGCTCTCACTTTCTGAAACTTTTTTTTCAGAAATACCGTTTTTGTGCTCGCATGTATTATTTAAACAATTCCATATATCGTTCAATATTAAAGTTTTTTTAATTGGTTCCTCCAAATCCATTAGAACACATGTTAAGTTAGTATTTTTAATTTCTTCAGTTACGTTTTTAATTATCAAATCAGATCTAGGTTCTTTGCCACATTTTAAAGGTTTTTTGTTAAAATACATTCGTTCTTTATAAATGTCGCCGTTCATAAATTCAACAAACGAATTATTATTTCCTATCAGCCAATACACATATGCATAATTGCTATGATGACTATATCCTTTACATTGTAGTAAAACGTTTTCGCCCTCTTTATGAGGAAAGTAAATAACTAAATCCCTTTTTTTTGCACATTTACTATAAACCACATAAAAGGTTGTAAATAATGTCAGACAAATGACTACTTTCTTGAAAGAGATCATTTTTTATTTTTTAGAACTATATTTTATATTTTTTTTTAAAAATAATGAAACAAACTTCTTAAATGACATCTAACGCCCTCATATCCTAATTTGCATCTGCAAAACATTAGTGGTTTTTTATTGTAAGATAAAATAAAAGTTGTTTTATAACATGTACCACCATTTAAACAAAAATTTATACTTTTATCACACATCATTATATCTTTGTTTAAGATAGTTATGTAATCGTTTTTATTTTCTTTAGAAAGCATTAATATAATTTTATAGAATAACAAAACGGTAAAAAATATTTTTTTTACTTCCATCGTATCCTACCAATTATTTTAAGCAAATAATATGTAATAATAATACTTCCTATCATTACGAAAAAGTTAATTATTGAAAATATAAAATCATTTTGTTCATCGATTTTTTTATTTTTTTCAACAAAACTTATAATGTCTTTTTGATAACTAGAAAATGTATTTATTAGCTCATTTAGTGCTAAATCATCATTAACAACTTCATTGTTTTCACTTTTTTTCTTTTTTATGATAATGGATAACAACGATATAGAAGCTAATATTACTGAAGGTCTAAAATCACTATTTATAGTTTCGATAATTATATTTTTTACATCATTTATATCATAACAAGATTTAACGTTATTGTGTAAAATAAAATTACATATTTCATTAAACTCAGTTTTATAATCTTTTTTTATTACCTCACAGCATTCGCGTATCATTATTAAAATTGCCAACTCATTATTATTTAATGATTCTATTCTTAAGTCTCGCAAGTACACATTCAAAACTTTTTCTATGTTGTAATTGCTGGTATCCATTTTTACAAAAAGTAACTTATATTTTCCTTTATTATAAAAAACTATAATATTCCACTTGACCCAAATCCATTATTATCTCTATTTGTACCATCTAAACATTTAACTTCTTTAATTTTTGGATATTCTACTTTTTCAAAAATAATTTGCGCCACTCGATCGCCTCTTTTAATATTAAATGTATTTTTACCATTATTAATAAATATAACTCCAATGTTCCCCCTATAATCTTCATCTATAACTCCTCCTCCTATATCTATGCAATAATATAACGAAAGACCAGACCTTGGCGCTATTCGTCCATAACATTTTTTTGGAATAGATAAACTGATATCGGTTTTAACTAAAATTCTTTCTTTTGATTTAACGATATAATCATAAGCACTATATAAATCATATCCTGCAGAAAAATCTGTTGATTTGGTAGGCAGTTTAGCAAAAGCAGACAAACGTATACAGTTAACGACAGACGAACTATGATCCATTTTGTTATTTTTTAAAATTAGGATATTTTTTTTTTGTTTTTTTAAAAAACACTATCTATGTCTAATGCTCCTATAACGTTCCATCCTTTTGTTTTATCGTCAAATATTTCTATTTCACGTATATATCTTTCATAATGAAAACCTCCAACGATCATAATACAATCATCAAAAATACATATTGATGAATTAAATCTTGGCTGATTCAGACACGACTCAATTCTCCATTTACAAGACATAGGATTAAAGCTTTCGACCATGTTAAAAACTTTTATATTATTGATATATGAAATTCCGCCAACAACATATATTAAACCATGATGGTATATAGCGCATCCGCCATAATGAGAATATTTTGTACACTCACCCAATGACCATTTATTATATTTCAAATTATACACTTCTACTGATTTATCATATTCAGATATTCCTCCAATAACATAGATGGTATCATTGACATTAACAACACATGGATTGTATCTAGGATATAGTAAATTTGGTTCTTCTCTCCACACACTTTCTCCAAATGACCAACTTTCAACTTTATTAACTATACAATTATTATAGATTCCACCAATAACATATATTCTATTATTAAAATAAGTGGCACCTGGGCATTTTCTTGGATAAATTAATGAAGGTAAGTTTTCTCTTTCAAAAGTTTTTACATTGATTGCGAATACACTATTTACTGATCGTAAGTATTTATCCACACCCCCTAAAAAGTAAATATTATTACCAATAGCAATTGAAGTACAAAACTTAAAATTTTCATCTATTATGTTGCTTAATTTACTTAAGTTACTGGGTTGTTGAATACTTTCATTTAATGTTTTGTTTTCATCAATTTCATTACAATTGTTTTCATACAATAGTTCATTATCATAAACACTTTCATCTATTATATTTATATCCATTATATTATTTGTAAACAGTATTTTTGTTAAGTTATCATTATAAACCTTAAACTTAAAAGACTTTGTAAATTTGTCACTATTAATAAATTCTTTATTTTTATAGTTACATCGTGGTATGTGTTTATCACTATTTTTGTTAAAATCATAAGGTTTTTTTTTAAATCTGGACGACCACGCTTGTAACCTTTTTTTTCCTTTTTCTGTTAAAAAGGTAAATCTTACAGCGTCATTTACTAACTTCATCGTATTAAGCCTATTAGTTTTTTTGACAGTTCCCCACTTAATTATAAAATTAAGAACAAAATCTTCGGCATCGACAACTAAATCACTACTTTTTAATATGGGCCTTAATTCATTAACTCTTAATTTAGTTAAATCGTTGCTTATATACATAAACCTTTTTTTAATATATTTATCTGCAACATCGTACACATCGTAACAACCATACATATAAGATCTTCTAAGATATCAACACAATTACTATCATCAATGTAATTTATCATAAAATCAGTACATGCATCCATTATAAACTTAATTTCGATTACGTTAGAAATTGAAAAAATGTCAACAACATTTTTGCTATTCAATGTAATATAACCAGTATTAATATACTCTAACACATACGTAAATGGCTTATATTTAAAAGGTAACTTAACTTCGTTACTGTATTTTTTAATAGCTTTTTTAAAGTAATTAGATGATATCTCGAAAATATCTTTCCTTGTTTGTGATATACCTCCAATTGTAAATATTAAAATTCTATCATAATCATTTTTTAATAACATGTACTTTAGTTCTTCTACATATTCTTTATTGATATACCTTTTTAAAGTCATATTGGTTATTCAAGATAATTAACAAGAGTTGTTATTTATTCAACTTTATAATATTCAATAAAAACTAAAAATCAACATCTAACGAAAAAACATTATCGTCTTTGTTAGAAATAACGCCCATTTTTTGATATTCACTAACTCTTTTTTCAAAGAAGTTAGTTTTACCTTCCAGTGATATATTTTCCATAAAATCAAATGGATTTGTTGACATAAACACTTTATCACAACCTAGTTCTAATAGTAATCTATCAGCAATAAACTCAATATATTGAGACATTAAAACACAATTCATACCTATTAGTTTTACAGGAATTATATTGGTTAAAAATTCTTTTTCTATAGACACAGCCTCGTTTATAATAATCGTAATAATGTCATTAGAAGGAGGATATTTTAGATGTTTAAAAATTAAACACGCAAAATCACAATGAAGGCCCTCATCTCTACTAATAAGTTCGTTTGAAAATGTTAATCCTGGCATCAATCCTCGTTTTTTTAACCAAAATATTGCAGCGAATGATCCGGAAAAGAATATTCCCTCAACTGCAGCAAACGCTATTAATCGTTCTCCAAACGATGCTTTTTTGTTTGATATCCATTTTCTAGCCCATTCAGATTTTTTTTTAATAAAATCCATTGTTTCAATAGCATTAAATAAATGTTCCTTCTCTTTAGTACTTGAAATGTATGTATCAATAAGTAAGCTATACATTTCAGAGTGTATGTTTTCCATAGCTATCTGAAAACCATAAAAACATCTAGCTTCTGACCATTGTACCTCCGAGTAAAATCTTTCGGCCAAATTTTCATTAACTATACCATCACTAGCCGCAAAAAATGCCAATATGTTTTTAATAAAGTATTGCTCTTCATTGTTGAGTTTTTTCCAATCGGCTAAATCCTTAGAAAGATCTACTTCATCAACTGTCCAAAAACTAGCGACAGCTTTTTTATACATTACCCATATTTCTTTATATAATATAGGAAAAACTACAAATCTTGAAGATGTTTCTTTAAGAATTGGTTCCATTTTATTTTATACCATAAAAATTATATTTCATTATTTTATTTAGTCAACATTGACGCCCACACACTTATCATCGATATAATAAACATTTTCTAATTCGTCAAAGGATCTTCTTGTGGTTAATTTCATCATTCTTACTTTTATTTTATTAAACAAATCGAATAAGTAAAAGCAGTTAACCAGTTGAAATAGCAAATAAAGACTTATTACGATAACAATTATTAAAACCATCAAAATTAGTACAGTTCCTATGCTAAAGGCGTTATTGTTAGGCATTGCTGTTGTAAGATTCATCTGATGCATTTGCTACCACTATTGTAAAATTTATACTTTTTTTTATTTTTTATAGAAATACATCATCATTTGAAACACTGTCGTCATAATTTATGTAATACATTACTTTATGAGGTGTCTTTCTTTTTGTAAATTTATACTTGTAGTTATAATTATATTTAGTATCATTATCATCACTACTAACGGTATCACTATCACTATTACTATTACTATCATTAGCACCATTACCATCACCATCACCATCTAACAATGTAACACGTTCAATAGATATTGCCGTTTTCATATTGCTTTTCCTTATAAAACTAACACATTTAATAGCCTTTTCTTTTATACTTTTTTTTAATAATCTGTAACCAAATACTATACCACAATAAATTTTTTTATCCATTTTTCCTCACTTACTATCAATAACGTATAACTTAGTTTATTTTTTTTGTTTTTTAACCTTAAATAATATATTAATTTATTTAGATAAAAATATTTATTTATTTTTTTTTGACGATTTTATACATCCTTCATAGTATAAAAGTATATAGTTATAGTAGTATTATGTACTTTTTGCAGTGTAAACTACTTATGTGCAATCAAAAGTTGAAAGTAAACATAAGAGTCACATTTTATGTATCATAAAACGAAGTATTTGTTGTTATTACCATTGGATAATCGTTATGCAGGTAGGTTTCTTTTTTATCATTGTGAGATGATGGTTTTTTAATATTTAAACAATATCCATATTTTTTAATTTCGTCTTTAGATGGGGTAGAGTTACTACTATTGCTGTTATATGGCTTTCCATTTACAACAACTTTGTTAACTTTTTTTAGTTTTTCGCTCATGTTTTATTTAATAAGTATTTATTTATGTTATTAGCTATGATTTATGTTTCATTTTTAATGATTTTTTAAAAAAAATTATACATATAAAAATGAACCGTATTTGTACTTAATGTTTATTTTTCGTTTTATAGAACATAAACCAAGTAAAAAAATAATAAAAATTATCAATACAAAAAAAATAAACCAATGAACATCGTTGATGAATGGTATCCTATTATCGATATAATTATTAATTGTATATCTATTACTCATTGATTTCATTATTGTATTCATAACACAATTTGCTTCAGAAGTTCCAGAATTAATTATTTGTAATAAAATGTTTTTTCCATTTGGTGCGATACACTCTCCAATATCGAAAGTTTGAATATCAATCACATTAGTTACGGCGGCCGAAGAAGTACATTTGCGTATTATTTCGGGTATATAATTTTTATCTTGATTATCCAAATCAATCCCGACTTCGTTCGCTAATTCATACTTCTCGTTTTGAGATAGAGTATTAGCAAACTCTTTAAATGTTTTTATCAATAAGTAAAAACTTAGTTCTTTATCATCAATACATAAATTTATTATTTTTAATTTACATCCTTTAAGCGTTCCCTTTATTTCACTAATGTGAATACCACAAGTTGTGTTAACCGGAACGGAGTATAATGATAAATTTTGTAGATAGCGCTCTATGAATAAATTATACAATGTATCAACTTGAAAGTGGTTTTCCATTTATCCACGTTGAGAAAATGTTTTTCTTTGAAATAATTTTTTCTAAAAAAGAAATAGGATGTAATATTGATATTTTTAACCTAAATTTATCACACGTATTTTTATTACAACATATAATAAATTCTTCTAATGTATTATAAAATTCTTTATCTGTTTCTATTTCTGGATATGTTTTTAAAAGTGTATGAATGAAAAAATGAAAATCGTAGTACCAATTATGTTCAACTTTTATATTATTTTTTATTTTTTTATTTATTATGTTTGCAACCTGTGAAAAGTCAAAATCATTTAAACAAGCTTTTATAGGTTCATTAAAAATATAAGTATTTTCATTTAATGATATTTTTATTGATTCGTCCGAATCAAAAATTAAAATATTATCAGGTTTTAAATCAACATGTAAAAAGTTGTTACAGCATGGTAATTCATATATTTTAACATATAAAAGAGCCATCTGTAAAAAAATAAATTTTATATACTCAACTAATGACTTAAAACCTAATTCAATAGCCATTTTTTCACTAACTTTATCAGAAGAGAATTTTGCCAAAGGAAATATAATAATATTTCCTCTATCGTATAAATAATTAGACCGTTTTTCGTGTTCAAAAAAATGAAACATGTGTGTAAAATAGTTTATTACATTTACATTACTTTGTATAACTATAGGGTAAAAATATGATAATAGTTTTACAAATTTTACATCTCCTTTTTTCTCATTAAACGATTTTAAAAAATATTTATGTGAAAAATTACTTATACTTAATTTTTGATCATCCATTGTTTGAATAAGTAATAATATCATATATAGTACCCGCTTATATAAGTTATATAAAAATGTAAGTTTAAAGTTTACACCCATTGCCCACGCACACACTATTAGTTTTTTTTCATCTCCCTTTAAGTTATTAAACAAGAACTTTGGTATTGTATATTCTGCAGTTGTTTCAATAGGACTATACTGTTTATTAGGTTCATAAACAAATTTTACAACATACTTATCAATTTTAAAAACTATACCATATCCTCCAGTTGAAATATGATAAAAGTCATCATTAATTGGATAAAATCTCTTATCTTTTTGCTGAAAAAACGATGGATTGATATATTCGTTTTCAGCAATTTTATTTAATGTTTCTTTTGTAAACTTTTTAAAGTATCTTATTAACCTAACATTAGGTGACCAACTTTGGTAAATATCTAATTGAGATATTACATAATCAAAATAAATATCATCGCCTAATACTGTTGTAGATTTTATATCTTCATCGTTATCCCATTGGCATTCTAATGAGTTATCATCTATTTCCATGATTACCATTTATTGTATAAAAAGCGTTTATTATAATATTATAGTTAATAATAATATTTTACTTTATTTATTATATAGTCGTTAATCAATTGATAATGTTTTATATTATAATCTAAAAGTTTGTCTATTTTTAAAAATAAATTTGTGATTTTTTCCCAAAGGGTTTCATCATCGTCGATCTTGTACTTTTTTTTGAAACTATACAAAAAGTTATTATTTTTATTTAAAATAGTATCATAAATAACAAAGTTGTTAGGAATATAACAATTATTTTCTTTTTCCATTACCTTTTTTAACTTTATAATTTTATTAACTAATTCGATTATTTTTAATCTTCTAATTGAAATAATATTTGACGTTATGTTTAATGTTTCTTCATTATTCTTTTCATAAAAAAATGCTTTTTCTGTAATTGATAATTCTTTAGGAGATAAGATTGTCACTAAATAAATTGATTCGCCACCTATTTGTGGTAAAAAATTAACCCCATTAAGAAAGCCACTCATAATAAAATCTCCTTCTTCTATATTTATTTTTAAAAATGTAGTATCTGGAATAAATAATCCAGATATTCCTTTATTCTTAACACAGGCCGTAATGACAACGCTATTACTAAATGTTGATTCGTTTAAAAAAAATGCATTTCCGTGTTTTAATTTAACCAAATACGATCCTATAACTGTAAAAAACTCTTCATTGAGTAAAAGTTTATAAAAAATATTTACATTTATTTCTTTCAATGAATAAAATCCTACTTTTTTTTTACTCATATCTAAGTCATTTAAAAAAAGATTAAAAGAAAAATATAACTCTTCACTTTTACTTTTTAAAAAATTACCAACTGCAACATTCCCACCATTAACTGAATATAAATCATCATAACATCTATTATTTAAAAAGTACTTCAAAATATATTCTTCTTTTTTTTTATTACTTAAACACATAATAGACATTATCGTATTTATAAATCATATATCATTTCATATAATGCATTTTTATCTTTTTTAAAATCGTCCGATATATATATTTCTTTATAATTATACATTAACACATAGTCTCCTAAAAAATGACTATCAATTTTTTGTATTAAAGAATGAATATTGAATCTACAGTAACTAATAGAAAATGCTTTAAATAATAATAAATAATTATTTAATGTAGTATTATTTTCATAATGCTCTAGTAATTCGTTATTATTTACTATATAGTCTACGATATCATCGCTTAATATTTCTTTTATATGATCAATAGTTTTCATAAAATCGTCGTTATGAAATGTAATACTGTTTTCCCATTCTAACACGCCTAACTCCTTTATTTTATATCCGCCTATTTTTCTCATTAATTTTATATCTATTGAGGTCAATGGAGTAAAAAATACAGATGTTTCTAATTCATCATCGATTATAGGAATCTTTACGTTATTATTTTTACTCACTTTACATAATGCTATATATAAATTGTTATTATTTATATCTATACTTCTTTTATAGATGGGGAAACCAATAGGGTATCGCCCTAAACTTATTATATCTAATATGGATTTAACTTTAAATATAACAATTTTTTTTTTAGGAGAATAATTGACCGTTTTAACATTTTTAAACCTTATTAACTGCATAATAAATCGTACAGTGTCGTGTTCTAGTGGATAATACATAAAATTGTTTACTTTAGATAGTGATGTTTGTATTATTTTAGTTAAAAAAATATCTTCTATTCTATTAAAAGAAAACATATTAAAAGGATATATTAAATTATTTAAAACATCATATTGTTTGTTGATGGAATCATATAATACCAAAACTGACTCTAATGCCATTTTTTCTAATGCTTTTATTTTACATTTTGCTTCTTCTTCCGTGATAAAATCTTTTGGTATAATTATCTTTTTTTTATCATTCCACTGTTTAAAATAATCACTTATTTCCATTTTACACGAAAAGTTAGCGATATCTACTAATATTAGTTTGAATCCGTCATCTGATACAATATAATCATTTCCTATAAAATTCCATCCTATGTTTTTTGGCCACGACGCTTTAAGCAGTAAAAAGTCAAAAACATTGCTTAAAAACCCTACTAAAAATGCTGTTTTAATATTTATTAGTATTGTTTCCTTTAACCATTTTAAAAAACATAATATTAATTCTTTTGAGTTAAAATTTATAAATGATTTACCAAAACTTGTCATCATATAAATTGATGGACCTAATGGAGTTATTATTGAGTTTATAATTATAGGTATTATTGTAACTACTTCATCATATATGTTATTTTTTGACCATAACTTTTTCGATGCGATACTTTTAACATTATAAGGTAATATGTCTAGTTCCGGTCTATTATATTTTTTTTTTTGTTTAACCCATATATAATGTTTTCCAATGCACCCTATTATCATTTCATCAATTAATGTATAATCATTACCTTTTTTACTATTTTTATAATTAACAAATCTATAATTAGATAATCCATTATCACACATCCAATAATTTATCTCACTCAATGAAATATTTTTATTTTCAAAAACATGCAATTTATGAACAGATTTATTTATTTTATCGTTTTCTAAAGCTCTTCTTTTGACAATATCTACCAAAGGAGATATAGATGTTTTAGGTGAAAAAAATGTTTCAGTGTCGACTTCGTCAATGAATAACTTGTGCATATGCATGTCAATAAAATATGGTTCTGCATACTTTGTAATTCCAACTATTTCTAAATGAGATAGTTTTGTTACAGATCGTACTAAATCTAAATTATTTAGGTATATTATAACTCCCTTTCCGCTTCCTAATCTCGCTAATATAATAGAGTGTTTATATGTTTCTAATGCATTAATAATATTAAATTCATTGTTTTGTTTTTTAAACATGTTTGGTTACAGCACTGTATTTTTTTTGTCTGACCAATCTCTTTCAAATATATTTGATAACATCTTAACTAATTCATTATCATTGGTATTAAAACTAACAAACGCATAGTGTAAATAATGTGTTCCATCAAAATTTGCAGGAGTTATATGTGCAAATTCGTTATCAATAATCATCAACTTGGTATTATTAGTATCATGAAAAATTTTAACAGTTAAATCATTGTTGCTACACAAAGATTGCAAACTTTTAACCGATGACATAACATAAACGTCATTTTTATCCCAAGATCCGACCAAAAGCCTAACTTTTACACCTCGATTGATAGTTGCGCCAATGATAGCATTGTAAATATCTGGCCAAAATGTTGTTTTTAAATCTTCTCTTATAACGGGAACCAACGATAACAATTCCAAATCTATTGACTTTTTCGCTGAATTAATTTTGCTCAACACAACATCCGCATCTAAAGTTCTAGAATATCCTAATATATGATCAGGAGAATCTGACAAAAACACACCACCAATTGGATTATTAATATGGTATTTTGTACTCACTGGCAAACAACATGTTATATATATTCCTCTAAAAATACTGCTGTTGTTATTTAAAGCTTTAAATGTTTCAAACCTTCTTTGTAAATCTAAAGCTAATGGCGCGTAAGTAGAATATATACCTAAAGTCTTTATATTGGAAATGGATCCACCAGTTAAAGAGGCGTTGCCTATATAGCATTTATTATAATCAGAAACCCAAAAACTTCCCAATAATACTCCCGGATCGTTATCTCCTCCTATTTTTACTTTGACATACTCAATTTTATTTGATATTAATTCCTCTTCATCCTTGTTACCGCTTTGATGATCTACTAAAATTGTAACTTTAATTCCAGATGCTGCGGCTTCTTTTAATTTATTTAAAATATTTTTTCCATGTTCGCTAGTGCGAAGATTACAACAAAATGATGCTATATTTATATTTTTTTTAGCTTGTGAAATAATTTCATCAAAGCACTCATGTGTGAGCATATGTTGTGTTGTTATCCCTGAGGTTTTAGGCAATGTTTCAACGAGTCTACATCCTGCTCCAGATGGAGGTTTTGAAAAAAATAAGGACCACATTTAATGCTAAAAAAAAAATAGTTATTTTATAACAATGTTTGTTTTTCTTCAATATGTTTGTAGTTATTATCTTTATTTATTTCTTTAAACATAATAACTAAATCTAAAATTATTGACACTATACCCACTAATGCAAAAATAGCACCAATAAAGACCAAAAAAAATTCAAACATCATTTAATAGACATGTTATTTATTTAAATTATTCTATTTGAAACTTTGAAGACCAAAAATGTTTTATTCTTAACCCAATTATTAAATATTTTAAGTCAAAAGTTGGTACAAATATATTTATATTTAAACTATTATCATCTTTATTAATGATACAATATTTACATATTGTTTTGTTTTTTGTTGCCTCGTCAATACATTTTAAAACAAAACTTGGTAATAAGTATTGACATTGAGGTTGTTGAAAATGAAATGGTGAATATAGTAACTCAGTTAATGTAAACCTATAACCTTTTATAATAACAGAATCTGATGTAGCTACGGAATTTGTAGGAAAGTGTTTTACTGTATCCATCTTGACAAAGCATCTATTTCCAAGTAAGCAATTATCATCTTTATTGATTTTTATTCTGTCCATATTTCTAAATGGACTTAATTTAATTATTTCATCTAACTTGTTAACATTCATGGTTGAGAATGTTAATGATGTTAGATTTATCTAAAAAAAACGAATGCTTTTTCATTTTTGATCGCATACACTTCTTCTACACTGTTATATTTTTAAAGGATGTGCATATATCGTTTACATAATGGATGTTATCTAAATCTTTTATTTTATTTACAAATTTATTATAATAATAATTTTTTTCCTTTATATTTTTTTTACTTTTTCTTGGATGCAATGCTTTCCTTAACCATAACGAATCACAATTAATTGCATCCATTAATATTTTAAAAGTTTCAAAGTTATCGTAAATAAATTTCTTTATGATATCATCACTACATGTATTCATATTAATTAAAACAAACAAATCTTTGAGCTTTTTTCTTGTATTAATTTTGCTATCAAGATTAATATGTCTAAATGATGGGGTTATCCCATCAAACAATAAATTATCTTTAACATAATATATCGTTAAGGATTTATTTCTTTTTCTCATTAAAAAGTTATATAAATGATCATTTGAAACAGATAGTTGTTTTTTCTGGTAAAATACCAATATATCAATTTTATTATCATGAGATATACACTCCCATCTTTTTGAAGATATAACTTTTTCATCTATATATCCTACCTCTTTTAGCTCAATTTTTATAGATGAATGTATTAAATTGCAATAGTTTCTACATATTTTTATTTGATAATCTATAGATGTATCAAATAACGATATTAAACTTGTTACTATAGCTGCATTTTTTAACATTTTTTTTTTAAAAACAAAGGTGATTTTTTATACTTTTTTATAATAGTTTTTCATTTATGTTATAGTAATATAAATGGCAGCATATAATTCACAATATTATGCAAACACACCATTTTATATAACGACAAAAGAAGGAAAATATCTTGTATTAAAAGCAATTAAAGTTTGCGATATAAGAACAGTTGAATGTGATGGCGATAAAGCATCATGTATTTTAAAAGTGGAAAAATCAAATCAAACTTGTGACAGGCCATCTTCCCCATGTGAACGAGCTGCAAGAGCTTCATCCCCAAATAGAGGGAATAACTCTGTACCATTTATGCGCACAAATATGTTAGAGGACCTTCAAGCGAAAAATCGTAGCGTTATGTCTAGAATACTTGGTTAAATTTTAATGCGTTATAATATCTTTTTCTATATCAATTATACCATGTTTACCACTTTTCTTATCTCTAGGTATAATATCATAAATTGGGTTTTTTTCTACCCCAATTAATGAGTTTACGATATCTGTAAGTGGTTTTATTATATCTCCTTTAGTTAAAATTTGATAAATTAATATATGTGCGCTTAATCCTCTGGAACTTATCTCATGAATATCGGTTTTTGATTTCATTAGTATTGTATTTGAAAAATAAGTATATAAAATATTATTATTTATCAAGAATGCAGAATTTGAAACATTTTCAAAATCAATAGCATCGTCAGCATTTAAATCTAATATATCACTAGATAATGAACTTTCATCTAAATAAACTAAACATTTTTTGAATGGGAAATCGTAATTGCTAGTATCGACCGTGATGATACGTTTATCAATGTCTATAACTGAATACATAGGCATGTTATTTTTTTTTGTTTGGTTACTGATAAAGTTTATACCATAGTTAACTCTAACATACTCTAATAATGTAGCTAATCTAACTGTTAGTTTTTCAGGATTTTTAGCTAAATCTTCTAATCTGTCTATTTGTGAAAACATTTTTAACATTGACATAAGTTGCAATGTTGGATCTACTATATATATATTATCAATTAAAACTTTTGGTATTGATTGCATGGTATCTTGTCGTATATTAAAACTATCTATTATATGGCTATCGTTTTGATCCTTTAACACCATATAGTTTTTTAAGTATGGTACTTTTAATAAAACAACATTTAAGCCAGTAATAAACTTGATTAAAAACGCAAGATCTATAAGAAAAGTTCTAGAGTTTGTTTGTAATATATCTATATCTCCATATTTAACATCCGGATTTAATAAATGTAGAGAATATGATCCATAACAGATACAATTTTTATTATGTCGTCGCAAATACTCTTCCATTAAAGAGTTTACATTATCAACTAACGATGAAACATTATGTCTACCCATGGTTTTATCCGTCATCTGTGCAACGTTCATAAAATTTAAAGCATCCCTAGCTAAAGATTGCATCGATGATGCATTATAGTAAATATTAGAAGTAAATAAATCAGGCCCTTTAGTTGTCAATATCCCTAATACATCAGTATATGTTGTAACTAATATACTTTGTAATTCTATTATGGTATATAACTTACCAATTGAGTATGTGTTCTCTTGTTTAGAAAAGTAAGTAAGAATTGATCTTTTTATATCAGATGGAGAAGACTTGATATCAGAAAAAAATTTCTTTTTATTCTTTTTTATCAAAGATATAAATATTTCTTTATTAAAAATGTTTACTCTTTGTATGTTTTTATATTGTGACTTCAACATGTGAAACTCGGTTAAAGATGGATCTCTTTTTAAATACAATTTTAAAACATCTACAACAATTTTATCATTCATTTTTGTTTATTCAATATGATGAAGATACAAACTTTTTCACAAAAAAGTAGTGTATTTTTTAAATAATTTTTTTTTATTATTACAAATCCACTATCACATATATATTTTTTAAAAAGATTTAATTCTTTACAAACGCTAGATATGTCTTCAGTAAGTGTTTCATTTATTTTCATTCCTGTAAAGAAATTACTTATAAGTAAAGATATAAAAACATTAGTTCCTCTACTAGAATACGTTGATCCTACTACTAAGTATAAAAAAAGAATTTGAATAAATGAATTTATTATAGAGTGGAAATTGGATATGGGTTCATTTAGTGAATAAATATTAGTTATATAATTGCCTAAAAATTTATATTCGATAAAATCTACAGGCGATATCTTAAAAATAACGCCGTGCTCTATCTTTGAAGGGTGTGAATATTTAGATCCTTTTATTATATCAATTATAGGAACCCAGGAAGGAAAATATCTATATGGTAAATATACCAAACCATATGTCGTTAACTTAGCAATTGTAAATACTTGATTTAAAATATCTTCTTCTGTTTTTAAAATAATATCATTTTCTTTATTTATTTGTATTTTTATTCCAGTGCTATCTTCAATATAAGACATATTTTTAAAAACGGTCATATCGATTTCTGGTATATTACTATATAATATTAATGATTTTATTTTATTTTGATCATATATAAACATACTATTTTTATTTGGCAATTTTTTAAATGGTATTAAGTTTTTTATATCACTAACCTTTTTAAGGATAAAATTAACATTACTTTCGCTGTGTGTGCAGGATAGATAAAATAACGACGGTTGAATTTTATAGCTAAAATATTTATTTATATTATTAGATGAAAATATATTTAGTAAATACATCGTAGATGTTTTATTTAATGGATAATCAAACATTAACTTGTTCATTTTATGAGAGCAAAATCCATATTTTTGGACAAAAATATCTATTAGCTTATTATTTTTATTATACCAATCAGTACTAAATGTGTAATCCGAAACCAAGTTTGCGTTTTTTTCTAATAATACATTTATATCAATTGTATCTAGTAGCTCAACTAAATCTTCGTATCCCATTTCAATACACACTATCATAACTAACGAACTATGTATTGGAATATCTTTGATTTTAACTTTAATATTTGATTCACAAACTATTTTTATACATAATTCTTCTGTTAATATATTTAATGGTATTTTATCTATAATAGTAGAAAGCAAATTGATATAATTTTTTTCTATTAAGTAATTGATAATATACATTTTATTGTTATAATCATAATTATCAATATATTTACAGCATATTTTTAAAAAATCTTTTGTCAATTCATATTTTGTAAAATCTATTAATGGATCTTTAAAAAAAAAACTATAATATAATCCTAATTCATCTTTTTTATCGTATAAAAATTCTATAGCATAGGTTTTATATTGATCAACTTCTCCATTTTTTATTTTAGTTATGATAAATTTTTTTATTCTGTCATCTTTTAATTGTTCAGACGTTAACATATCTAAAAATGTTAAAATATCTTTATGAAATTTTATAGATTGTAAAACATCCAAGATAAGTTCAATAGAAACCACGCTTAAAATTCCATCATTGTTTGGTATTATTCCATATTTATATAACATATCCATTAAAAATTTACTTTCAAAGGATTGATGTTGATATAAACTATCACATAAACTAAAGTCATTAATAAAAATTTTTTCTGCCAATGATTCACTAAACAAATAGTTTATATTTTTAATACTTTCCGAATTTATTTTAGTTAGTATATTTTCTACTTCAATGTTAGAAAATCTGTGTATTAGACAAAAAATATCATCATCTGCAATAACCATATACTGAATACATTTTAGTATTATTCTTTTACTACATTTTTTAATAATATCTTTTTTATGGAAATTTATATGTTCTCTATATAACTCAATATTTTTTTGATTATAAACTAAAGCTATCAAATCGACAAATGGTACAGTTTTTGGTTTGAATAAATATATTTTGCTAGGAAATTCTTTTCTTATCCTAAAATACCATTTTTGTGGCAAATTATTGGGGTTAATTCCATTAATGAGCATTTTCATAGCATCATCTATATTTACTTTTTTATATGATTCTGTTAAATTATCACAATTATATAAAATAAATGCTTCCTTTAACGGAATTAAAAAATTAAACATATTATGTAAATTTAAAATTTGATAATAGATGTATTGATTAGAAAATCAATAGTTCGTTTCGTCGATTTTTGTTTAGCTTCTTTTTTTGTATTACCAATTTCTGGAAAAAATTTTATACCGCTTATAATAACAGAGGCCAAAAACATTGGTTTTCTACCATTTCCGCAACTAGATATATTTATGTACCAATCTCTAGATGTAAACTGACAATATTCGTTTATCGCAGAACAAGGATTTTTTTCTTTCCATAAAACAATTTTATAGTACGGAATTGTATCAGAAAATGATTTATTTAGTTTTTTCGTATCGTTATTCTCATTGTCATCGTTATCGATGCCATTTTTTTTAAACCATTTAGGTGGATTCGATCTTATCATAAAAATAAAACCGTCATTATGTAGTTTATACAGCTGTTTGTTTACATTTGATTTTTCAATATTTAGTTTTCTAGATATTTCTATTGCTGTTATAAACTCACTTTCTGATAGATTATTTACCATTTTTTTCACAAGTTCATACGAATCTACTTCATCACAAGAATACATTTTTATTTTTGTTGTATTTAAAACTAAAAAATTTTTTTTTTTCATTTTTATAGTTTATGTTATTTTTCTACAGCTCTAAACTTTGGAGGTTTAAAATTTTTCTTGCAGTCTCTACAAGAATGCATAACTAATGGTGGTTCATCTGCTGCTCTTGTTTGTATCATGACGGGAATAGTGTTTTTACTTTTGCACTCAGGGCATGGGAGGTTATACTTTTTATCTAAAACATTAAAATATTCTTCGTGGTTTATATCTCTAATATTGTCTATATTGAATTCTACATTTTTTTGAACACATTTTATTCCAAAAAGTATATATCTTAAAATTTCTTTCTCTGTTCCATTTGTCGTACGTATAAGATCACATATGTCAGAATAATCTTTATTTGTCTCTATTAAAGGCTTGTTTCTATACGATAATTTATTTTTTGAATCTTTTGAGTATTCAATACCTATGTTGTTTCTTGGTTCAAATTTTGTTTCTTCTATATTTGACTTTGTATTAATAATATTTCTTATATAAAACTTGGAAGCTTTGTCTGTTGCCCATTTTAATATTTCTTTTACCTTTGATTTTTCTTTTACATATTTATAAATTATATCTTCTATGTCTTGATATGTGGGGGTAGTATTATCACTATATGAATTAGTATTATCATCATCCATATTTGTACTGAAGTTGTTTAATTTCAATATTTACAAAAATGAATTTTTAGTTCTTTATTTAGATTTTTCCATTAATGGCAAAAATGGAAAATATTACACCACCTAATCACTTTGAAAAAGCTTCTGTTTATGCATGTAGGTTATTTAAACAGTTTTATAACGAAAAAAAATATGATTTAGATGAATGTTTTTTAAAAGTTAGAAATAGCATGTCTCGATACTATTCTATGGTTGGTGATGATTTAGTTTGGAACAGAGAAATATTAAGTTACTTATATGAATACATAGCATATAAAAATAATGCAAATAATCCAAATTTTGTTCTATCTTCAAAAATTGTTAATTTAACTAAGAAAAATACAAAAAAGATAAAAAATGAAATAAATTCTGTTGTTCAAACTTACAAATTGGATAAACATATAAAAAAAGTAAATATAACACACTATGATTATATAAAAATAAAAAAAACGTATGATACATTCAAAAAAAATGTTGATATTAAATCAAATGATATATCTGTAACACTATTAAAAATTTATAAACAACTAAGCTTAATTAGTGATGTTTCGATAAATACACGTTCTATGACAAAATCTTTAATGTATTACATGTTTCCTGATCTCTTTAAAAAGGATAAACGACATGTATTTTACAGAGTAGACGCTAAAAAAGGATATTGTGTATTATCATCTAAAAAGATAAACTTAATTAGGGTTTTATTAGAATATAAATTTGATATAAAATACAAAGAGTGGGGATTAATAAAAAGATATATTAATACTATTTGTAATAAAGGTAGTAAAAGTAGCAAAATCTGTATTGGAAAATATCCATATTTAAAGTTAAAACCTGTTTCTAAAATTAAATTTAATTATAGATCTAAATACGTAAGTGATTTTAAGAGAATAGTGAGTTCTAGTTTTTCATTGGTTGATTTGACAGAAAAAATAACGAAAAAAACTTTTAGTAATATTTTTAGAAATGAAATTAGTCATCTGTATAAGTTTAATGCAGAAAAAAACCATCTCGCGCTAGATGAATACAAAAAAATGAGAATGTGTAATAAAATAATATCTGCTATAGATTATCCAAATAAAGATCACATTTATAGTGCGATAATAGAAACTGTTAACAATTACTTAGATAATCCTCCGAAAAAGCTAGGATACAATATTGATACTTGTTTGACTATAGCAAAAAACAATTAACAACTAATCATGGATTTTATACGAAGAAAGTATTTAATATATACAATAGAAAACAAAGTTGATTTTTTAAAAGATGAAGTACTAACAAAGATATCAAACTTTACATTAAATCATATATTAGCCATTAAATATTTAATTCTTAACTTTCCTAGAGATGTTATATCAAAAGATGTATTATCGAACCCAAATTTTTATGTTTTTTTACATATGGTAAAGTGTGATGACATTTATGAGACTGTTTTAAAACATTCGTTCGATATTCCTACGTTATATATAAAAGCACTTATAAAAAATTATCCGTTATTTAATAACACAATAGAAAGATATAAAACTTTAGTTAGTGAACTTTTTTTAGACGATAAATTTATCGAAATAGTAAAATATTCGTCTGAATTTGATAATATCATAGCTGTTAATTACGATCTTTTACTAAATCCTTTATTCCATAATAATGAACCGATTAAAAATATGGAAATTATTTATAGTAAACTTTTTAAAAAATCTAATTTTAGGAAGGTAAAAAAAATGGAGGTTATCAGATTAATGATATGGGCGTACCTAAGTAAACAAGATACTGGATTAGATTTTACTGATTTTGACTCTCAAGATATATACACACTGTTTCAAAAAAGTGATAAAGTTATTATTCATAGTGATATGACAGAAAAGTTTAAAGAGTATATTTTCGGGTCGGAAAAAACTAGTTATTGGTTATGGTTAAATGAATCAATATTTAATGATAATGTCTACAATGAAGGGTTTGCAGAATTAATGTACGATAAAATTCTTAGTTTTATCTATTCAGAGATTAAACAAGGAAGAGTAAATAAGAATATGCTGAAATTAGTCTATATATTTGAAAATGATGAATATATACAGTCTATTTTATTACAAATAATTTACGGGATACCTGGTGATATCTTATCAATTATAGATGTAAAGGATGATAACTGGAAAAAATATTTTATAGGTTTTTATAAAGAAAATTTTATCGATGGAAAAACTTTTATAAGTGCAAAAACATTCAACTATGATTTATTTAAAGTAGTGGCTAAAATAAATCCTGAGTACTTTGACCCAGACAAAATAATATCCATATTTGATCATAAACCTGAAAAAGTTAAATATTTTGATACAATAGATATAAATAATACATTTATATCAAATATAATTTATGAAACGAATGAATTAAATCTTTCAACGATTGAAGAATTACAATCATGCCAAATATATAATGAAGAAACAGAATATTTCATTAAAGAATATAATACTTATCTTTATTTAAAAGAGAAGGATCCATATATTTTGTATAATGGCATGTTAACAAATATATCAAAAGTTCCATCAAATAAAAAGTTTTCATTGTTTAGTAAAAACATATTAAAATATTATATAGATGGAAAATTGGCAAATATAGGTCTTGTGTTACCAAATTATAAAGGTGATATATTGGTAAAAATATTATCACATTTGAAATGTGTAGAGGACGTTACTGTTTTCATTAAATTTTCAGTATGTAAAAACTCTAGCATACTACCATCAATAATAAGAACAATTTTAGCAAACTTTAATATTTCAATAATAATATTATTTCAAAAGTTTTTAAGGGAAAACTTATTTTACGTGGAATCTTTTTTAGAAAAAACAAATCATTTAACTAATAACGATAAAAAGTATATACTAGAAATAATAACTCGTGGAAGATCTTAATTTAAAATGATGGGAGACATTTATTCAGAGTCACAAAAAAGAAACGACGATCAAAAAACATACTTTACAAGAAATTTAAGTCCTTTAATGAAAAATACATATTTATACCATAATTATTCATATGGATGGATACCGGAAACAGCTATTTGGAGTAGTAGATTTGCAAATTTAGACGTAACAGAGTTTTACCCAATTACAATAAATTTAATAAAAAAATTTGAATTTATGCTTTCATTATACGATGGCCCCATATTACCATATGAAGAAAAAATTAATACAGAATTTATTTCAAGAGGTTCTTTTTCTGGAAGATATATAAATTTTTTAAGAAATTTTTCATTACTACCAACTAGTGAATTCATTTCATTTTTATTATTAACATCTATACCAATATATAATATTCTTTTTTGGTTTAAAAATACTCAATTTGATATTAAAAAACATACACTTTTTAGCGAAGTTTATACCAATAACGATAAACATATAGAACTAGGAAAATATTTTAGACAAACCGGTGATTATAAGCCTTTATTTAGCAGGCTAAAGGATAATAATATATATACTTTGCCGTTTCCAATCAACCAAAGTAACATTAACAACCCAACATTACCTATTGGCTTTTCCAAATCAGATTATGAAACATTATCTAATTTAAGCGCAATACTTTATTTAACTAATTATGATCCTGTTTTGATGTTCTTAGTGTTTTATGTGCCTAAAATATCTATAACGACAAAAATAACACCAGCTGTTAAATATTTAATGGATAAACTAAATATGAATGAAACGGATATAACATTAGTTTAATTATAAAAAATAGGTCTAGTTCCGAACATTTTTTCAAAAAATGATATGGATAATACTTTGTTATCTAACAAATTCACTATTTCAGTGGCAAGCCTTTTAAAGTATACTTCGTAAAATATTCGTTCAGTAGATTTTAATTTAAAACTCCTATCTATAATTCTTTCATACGTTTTTATATTTACTAATTTTTTTTGCCATGGTTCTTTTTTATTGCATATGTATGCAAAGTAATATCGTTCACCTATTTCAATAATTTCATCATTATTTTTATTATATTCATTTACTAAGTACATATTTGGATTATCACTTGATTTATAATTACAATGATGTGTTCTACTCAATAGAAACATATCCAATGGAGCTGTTTTTGTTTCAAATTCTATTTGTAAATCACTTTCTAACGATGATAAAATTTCAACACATACTTGTACAGATGACATATTTCCACCTGATAACATTTGCAAAAGCCTTGTTTTGTATATTCTTATCATATATTTGTGAAACTTAGATACGTCTCTCCTCGTCTCACTAGTTCCCTTATTGATACGTTCGGGAATTGATTCATTGGTGAAAGATGGCAAATACTTAAGGGTGGTATACTTTTTCTTCGATTGCATTATAAGATTTTTATATACGGCTTCGAACTCTATTTTGAAGTTAGAAAACAAAATCCTTTCATTTATAATTTTTTCAAGTTCTTTTGCAACTCTTATCGATTTAGATATATCATTTGTATCCATTTCTAAAAATATAGAATCTGTGTCCCCATAAACACTTTTAAAATTAAATGTGTATTCTGTCGGCAGTTTTGTATCTATATTAATATCTATATTTCTACCATCTTCAAAAAATGGATTTATAGGTTTTGTAGCTAATAATACTTTTCCATTTTCTACCTTTGATCCATTTAACACAGAGTTTAAATAATCAATCATCTTTCTTCCGATTGCTGTACAACTTTTAGCTGAAGAATATGAATACAATACGCTATTTTTAAATCCCATTAAACCATAAACAGAATTGGCTATTATCTTGTAAGTATACTGCATGGAGTCATAAATAGATTTGTCAGTCGACAGCGTTGCGTCTTTGTATTTTTTTTTGTATCTAGCCCTCTCTGTTAAAAATGTTTTTAAAAGTTTTGGAATGATGCCCTCTACATTCCTATCAAATACAGCAATTTCAGAAATTAAATCATCGCATCTAGGTTCACAATGGATTGATATATAACGGGGTGGAGGATACTTTTTTTCTATTTCTTGTTTGTTTATTTCTGCTTCTAATCTATTATTTGCAACAAATACAGATACTAATGTTTCAGGGGATAAGTTTCCAAATAAGCATACATTAGGGTATAAACTGTTATAGTCAAATATCAATACATTATTTATAAACATTTTTTGTTTCGGTGCAAACACTTTACCACCTTCATAGTGATATTTATTTTTTTTTTCTGATCGTGATAGAATTATTTTCTCATCTAATAATAAGTTTAATAGAGGTCCTTTTATTAATGTACTAGCTCTGTATTCAAACACCATGGATTGTGGCAATATATAAGTAAAAGCTCCAGCATCTATTTTTGTTTCAATTCCGTAGTAATTCCAAAGATATTTACATAAACAGGCGTCATGGATACAATATTTTGCCATCTCAATTGATATATTCAAATCATAGTTTTTATACATATCAGATAAACTAACATCGTCTTTTCCAAAAGAAATAGTGTAAATATCATTATCGATATTCTTTTTCAAAGTGTTATTTTTGTTTAAAATAGTTAACTGGAATTTATCATTACCAACTGATTTGTCTACTATTTTAAAAACATCATCTACAATAGTTATATAATTCCCAGTAGATAAAACTTCAGAAAACGAAGATATTTTTCCTATATCATCTGTAGTATTATCACCCACGAAGGTAAGCAAATTGTCGTTTTCACTTTTTAGTTTTACTGAACAACTAAACGCATTTTTAGATATAGAATCTAACTTATACGAATCCAATTTTTCTGACTTCTGTATATATGTATATAAATCAAAAAATGTTGTTCCGTTATTATTATTTACATGATATGTAGTATTTGCCACACCACCAACGCCTTTATGACTAGACAAGTTTCTCTCATATATGCATAAATGTACATGTTCTTTTTTATCTGGAGATTTAAAAATGATTTTCCCACTTGTTAACAGCTCTAATCTGTTTGAAATATACCGTAAATCAAAGTTGTTTCCGTTAAAAGTTACTACATAATCGAATGTCAATTCCAAAAGCTTTTTTGCAACTTTTAGCATGGTAATTTCACTACATATTATTATTTCTTTTGTGTAATCTACATCAGAAACAGATTTAGCTTTGTAATATCCTTTCAACGTAGCGTCTTTCTTATCTTTATTTGATAAGATATCTTCGTTTATTAAAGTAAATTTAAATTCTAAATTTGTTAGGTCAATATAACAACAACTAATATGAGAAACTGGATTTGTAAAAACGGAAGGAAACTTTTTATCAAACTGGCATTCTATATCTAAGAAAAGATAAGATCTATTTATGTTAAATCTGTTTATCGGTGTTGCAAAACATTCTTTAGGACAATCACAATGATAGCATTGTTCATTAATTTTTTTTAATTTTTCTACGTTAATACTATAACAACCTTCTGGTGATATATTATTTGATATAAAAAACCAACTAACGTTAAGAAATTCTTCCATGATCGCTTTTGGAATATTCCTTTTTTGTGGTTCTTTTATCAACCAGACGTTAACGTTTGTTTTCTTTTTTTCTACATCGGTAACATGATTGCTAACTCTTTCACTAATATCTATTAAGTTCATATTTCCTAAAAAATCAGAACTATATGCTGGTGGTGACAATGATATGTAAACATCTTCTGTAACTGCGTAATAAAAGTAAAAGTTAAATCTTAAAAATATAGTTTCTGATTGTCTATTTCTGGCTTTTAAAAATAAAAACCTTTCGTTTCCTTTACTTTCGAACCAGTTTATGCACTTTATTTCCATTTTTTAAATAAATGAATAAATTTTCATTTAAGTATGAATCCTAAACACTGGGGAAGAGCTATTTGGACAGTGATTTTTATCGTTATAACAAAAACCAAAACTCATAAAAATTTAGAACTATGCAAAAAACATATATATACTATAATTGAAACTCTTCCTTGTCCTATGTGTAGGATTCACGCAATAAGGGAAATAGAAAAAAATAATGTTATGTCTAGCGATGATTTAAATTATATTTACTTTTTTTTTATTAGTTTATTCAATAATTTAGCATCTGATGAAAAATATAAAATCGATTTAAATAAGGTATTACCGCTTTAATAAAACTTATTATTGTTAGATGATGAAATAACTGATAGACCTACTTTTGGATATATGTGCATCGACATTGTATCAGTAACTATAAATTCTATACCCGTTATTTTGTTATAATTATTAACGAACATTAGTTCACTATTAAATGTAAAAAATTCATTTTCAATTTGTGATATTTTCCCTTTATCATATTTAAAAATAAAAATATCTTGATGTTTAATTGCTAAATAAAATGTTGATTTTTCAACATTTATGTATTGCTTAATTATATCAATAAAAGTATCTATTGATTTTACCACTGAACTTTTTTTACACACACACTCGTTGGTATTATTTTGTACCTCTATTTTAACTCTGCCGCAATCAGATTCTAAAAAAATATTAACTAATTCCATTTATTTCCTCTAATATAGTAGTAATCACTTTCAAAAATATATTAAGTAAACGAACTGGAAAAGAACCGTTTATAACGATTTCGTTTATTTCTGTTTCAATTATATTACTTATATTTATTTGTGCGAAGAAAACACCAGATGCTTCCAAACATGTATTAACTACTTTTGAAATATATTCCTTTAAATTATTATATCTTTGAATTGTAATAATTCGATACAGTACAAACGATATCATATATTCTAAAATTATATTAAATAAAATAACGGTTTTTGAAAAATAATCATTTAGTGAAAAACATGTAAATGGAACAAATGACGATAATGGTGTCTTTGTTATTGGAATAAGTTTATTTACAAGGTAAGATCCAGATAGTAAGATATCTCCATTGGTGGATACAAAATTAAAATCCATTGTATTATAAATATTAATATCTATTTTATTTTTTTTTGTTCCATTAAGTAAACAAATTAATGCCCCCCATCCCTTAATTCCAATTAGTGATGGATTTACTAATCCATGAATAGCATAATTTACTAAATCATATAAACAGTTAATGATATCATCATCTGAAGTTTTTTTACCAAGATAGATATTTTTACTTTTGTGATTTATTTTACTATTGTTGCAATTTTCACAATTGCAAATTAAGTTCATATTAACTAAATTTTCTAATGTTTTTATTCTGTCATATATTTTTTTGTACTTGCATAATATTACAGAGGTTATATAATTTGTCATATCGAAAGTAATAATATCATTGATTTTTTTAAAACTTTGTATACCAAATAAAAATTTATTTGTTAAGTTATAAGAATATTTCCATTTTTTGATTATTTTTAGAAGAAGCAGCTTAGTTTTTATAGAAGAATTAGATAAACAAATATTCCTAACAATACACGGATTAAATTTAAATGGTGAAATTAAAAATTCTACTTCCGTTATTAAAATTTTTCTTTTTGAATTATCTATATAATTTAGTACATCATCAATCGTTTTTTCCTCATCGTCAATGTTATTATTTTTTTTAAATGATGGAGATTTAATTATTGATGGGGGTTTATACAATTTTAATTGATTGTAAAAAAATTTTTTTTTGAAATCGAAAAAATTTTTACACTTTGATTCATATATATTAATATTTTCTAATATAAAATTTTTATCATCGTCAGATATTGTTTCCGGATGAAGCTCTAAATTAAACGAAAACATAGCCACTGATTTAATTCCATATCTTTTAATAAGTTCTTCTTTAGAAACTGTTGAAAACAGGAAATCGTTCATATGAGTGAGCATCCTAGGGAAATTGTCGTTAACTATTTCATGAATTTTCTCAATAAAAGACAAATTTAATTTTATATCACTGGATACGTACGTAATAATATCATCATTAGGATACTTTTTTATTATTTCTATAGCCTTTTTCATATTATTTAGTTTTAACAAAGAAATATTTGTTGGTGGTACGTTAAGTGCATCAGATATATATAGCAACGTATCTAATTCTATTGACAAGTGTTTATATAAATATTTCCAAGTATATGATGGTGTAAATTTTATAACTTCAATTAATTCATCCTTTGATAAAATCATTGCGTTGGATACTAACATTGGGTTTAACTTTAATATATATGAATTAGGAACGATGCATTCGGATACTATGTAGTTTATGTCATCGATATTAAGCAAGTCGTTTTCAATCATTAATTTTATTAACTTATCACCATTCCTTGACGTAATCAAATCTTTTCTGTATGTCAACACAGCATTATGTAACTTACTATCAATTGTGTTCGATTCCAATAAAAGATTTATTAAATCATCCAAACGTATACTTTCAACTGGAAAGTACTTTAAGTTATTAATATCAAAATCAATTGCTCTATTATACAAACTAACTGGTAATATACCATGAAATCCAAATTCTAAAATTGTATCAACGAATACGTTTGAGTTTAAAAATCTTTCTATAGTTATAGATGATGAAATTATTTTAGATAGAGCATCTCTTGCCCGTTTGGGATATATATAAGACGATGACATATTTATTATATTTTATATGTATAAGGTTTTAAAAATGAATACGATATCCATACACAAAAACTAAACGCTATAAAAAAGAAAAATAGTACTATCATTTATAATGTATTATTCTGTTAACTTTGCATCCAATACAATATTTGGGGTTATTAAACACTCAACAACTTTAGATACATTAACTTTTTCGTTGTCTTTTACAAATTCTGATATTTTTTCATAAAAAGATTCTTGAAACTTTTTAGAATACCTATCCACATTAGATGAAATAGAATTCATTAACATACTATGTTTAGTATAAATAAAAGATCCAATTTTTATTGTGTCTATTCCTAAAATATCTGCTATTTTCTTATCTTTTAAAATAACATAAATTTTTGATTCTTCTTGTTTGGTTATCCTATTAAAATAATCATATAACCTAGTTTTTGATATTAAATTATCTTTAACCTGGGGACATTTAAATAATCTAGATTTTAAAACTTCCAATAGTTCATCTGATAGGATATATACATTATCTTTCACAGAATATTTTTCATCTAACGTTATATCATTATTTTTAGCTAATTGGTTTATTAGTTCTTCTATTTTATCATTTTCTAAAACATATCTATTTTTGTTAAATATAAAAAAACTTGCAGGCTTTTTTAATCTGGAAGATAATATTTTCACATCACTTACATCTATATAAAGAGAAGAAGATTTAGATATTTTTTCTAAAATCTTTTTTTCAATATCATTTATAACTAATTCATTTATAGCATCATCATTTAACTTACCATCCTTAGAAAAGTAATTATTTTCAACTAAACTATTGATTAGCGTAGATAGAATGCCATTTTTATAGTTATCGAGTTTAAAACGCTTAACGAAATCTTCTCTTATTAAATCTATTGGAATCCTTGTTTCTATTCTTTTAGCGAGTGACTTTTTTTTTTGTGGATACTTTTTAGTTACTAATTCGTCTACAATATCATTAATTTTAGAAGTAAAAAAAATCTTCAACGCTCTAGCACTAATACTATTTAATACCAATTGATCGTCATTATCCGCCATTTAAGTTCATTTTATAACTTTTAAATAAAAAATGATAAAAATTATAACAAAAACAATCGTAACTAATCCAATAATTAAATAATATCTTAAAAATGATTTTTTCGTCTTTTTGTTATAATTTTCGTCGTCGTTTAAAACTGATTTGACCACATTTATAAAGTTATTAAAGTCATCATCTGACGATGACATAAAAACTCCAAATATTGCAGAATACAACTTATCCATTTATACATTAAATAATGTTGATGTTGGATTATTATCATCTTCTTCTAAACATTCATCTTCAAGTTCTATCACGCCTAACCCTCTAACTGAATCTTCTTCGGAAACTTCAGCCATGGAAAATTTTTCACTTTTACCATCTACATCAACAATATTAGCACTAAAGTCTCTTTCCATGATTAATTTTTTTAATGCAAATGTAACCTTTATTGTTTCAGAATTTTTATACAAAACAATTGGTACAATTATAGCTTTTGCCATTTGTTTATCTCTTTTTGAAAGGCTTTCTAATTCCTCTATCATAGACTGAACTATTGATCTGTTGCCAGGAACTTTATATTCAATGATAGCACCATTCATTTTATTTATATATAAGCATCCCTTATTAAGTGTTTTTTCATCTAAATCATTTATTGAAATATTCACCAACGACGGTGGCACCTTTTTACCCTCTTTTATATTTGTATAAATATTTTCTAATAATTGATATAGAATAGGGCTAGTTGCAGAAAGTTTTATTTTTAAAAAGTATTGTTCTCCAAGTTTTACATCCTTTTTGTTTTTATATATTTTTGCTTCTCCTTCTACGGCTATATATGTAGATGTCATTTTCGACGATAATGCTTCTGTTAAGTTAATCATTATAGATGAGCACGAGGGATACTGTGATTGTGACAATATAACACTTTCAACACATTTTTCTTTACTTTTACTTAATGTTTTTAAATACTCTATTACTTCGTGACATTTTGAATACTTTTTTTCATCGCTTTGAGTTGACATAGCGGGTGTTTCTTTTTTAACAACCGCCGACTTTTGTATACTTTTACGCATTTTTATTATGTTTTAAATCTCAATTCGATAATATTTCATTTTTTCTTATTTAAATAATGTTTTTATATATGCAGAGTATAACGAAACAGTGCCTGGAACAAAAATAAATATAGCTGCGTACTCTAAAAAGCTTAATATTTTGTCAAACGTTAACGATCTAGCCATCATAAATCTATGTGGGGCGAATGATTTAAACAGTAACGCGCTACCACTAACTATCATAAATATAGCCAATAGCGTAACCGTTATCAAAGAAAATGTTTCTCTAGTAGATAATGCCATTTAATTATTATTTTTTTCTAAACAATATATTGTCCACTCCTAGTTGTAAAAGTTGAATTATTAAAGGCACTTTTACAAGTTATGTATCTTTTTTTACCATTCGGTGATATTTTTAATTTAGGCACATCAAATGTCGATTCTATTATTCTGTATGTATAGTATCCTTTCCATATAATATTTTCTTTTTTAAACGTGCCAGAAAATGAAACATTTTTAAAAAAATTATCTTTTGTTATAATCAAAATTGTTGATAATTTTTTATCGTTGATATGTGTTATATAATCTATTCCATTTTTTGACAACGTTCGTATTTCAACATTAGATGGTAAGTCTATTAATGATATCATTTTTTTAGGAATAAAAGTCGTTATGTTCGTCTGAATAACTGATTTAGGTTCTTCAAATTTTGTTTTTTCAAAAATTTTTATAAATTCACAAATAAAAAAAATTTTTTCTTCAGGGAATTTTTTATTTTTATTTAACTCATTATTAAAAAGTTTTATTGATACGAATCTAGTAATTTCGTCAGATAAAATATTAATTTCCGAAGATATTGGTTTTAATACTTCGATCACATAGTTTAAGTTATCTTTATTAGTTATATAAGAAAGTCCACAATACGAATCATCAAATATACCAAAAAATAATACTATTATAATTGACGTTACATCAAACGGAAATAAAGATATAACAACCGAAGGAAAGTTATTAGGCGTTTTAAAACTTAAAATATTAAGTTCCTTTTCTTTTTGTATTATTAGTAAATCGAAAATATCATAATGGGATAAAAAATCTTTTATTTTATTACTTAGTGGTTGAAATGGTTTATTATTTTCAATCGTTATTTTTATAACTACGTGATTATTATTTATATTATCATCGTTGTTAATATTATTGCCAACAATCCATCCTATGGGAATGAAAAAAAAATCCGTTATATAAGATTTTTTTTTTAAAATATTTATCAGTCCCATAATAACATATTTTTCATACTTAAAGGATTCCATTATTAACCATGATTTGTTTACATCATCTAAAGAATTGGTAGGTTTATTGAATAAAATTTCATTGGAATAATAAAAATTTTCAAAATTAAACGATATTATACATTCTTTTAGTTTTATTTCATCATCGAGTTTTTTAGATAGTTTTTTGTTTGGTTTTAAAATTTTGTAACTTACTTGTTTTATAAAGTTATTCATTATTAACTATTTTATTTGCTTTTATCACTATTTTATTGTATAACGCAGTTATTGCCTTTTTTGTCCACGCCTCCATTCTTTTATATTCTTTCAATGCGGGGCATTCTAAATACTCAGTTTCTATATTTATTTCGCCTATATTAAACAAAATACTTTTAAATAAAGTCATCTTTTTATCTGCTAAAAACTTAAAAAATGTATATATTTTTCTTATTGATTTAAATCCTTTCGGTGGCGTTTGTGTACAAATTATCATAAATATACTTATGAACATTCCACATTCGGATTCTAAAAGTTGGTTAACTTCTACATTTATACAACCTACCTTTGCTCCAAATGAATATTTGAAAAATCTAAACAAAACATCTACATCACCATTACCATTCTCTAAAACTGAATCATCTTTGTCATTTGTATTAAAGCCATCAGAAAATGAATAAAAATAAAAATTGTCATAATGATGAAATTCACTTGGTATGTTTCCTCCAGAATCATAAAAACAAACAACTAATTTTTCTTTATCAAAAATTACACATTTCCAATGAGACAAGTAACAAAATCCAAACATAGCATATCTATATTTGGATTTTCTTATTTTATCTTTTAAGATGTATGATAAAATATGTTTGTTTATAAAAAAATCAAACTTTTCATAAATTGGAACATTATTTAAAAATCTCATATCGTATTGTCCTAAATAATCAACATCAGTTCCATATGAAAATTGTGAAACTAAACTTTGAATACTTTTATTACTCATCCATGCCCGTTTATCGGGTTCGATTTTTATCTTTACTATTTTGCTATTTTCTCCCGCTAAAAATCCCTTTTTAGATAAATCATCAACTTTTATATCCATTTGCGAAAAATCGATCGCCTTCGAAATCTTTTCTTTAAGTTTAGGTTTAAAAAAATATTCAATTGGTATAGAAGTCGGAAAATTGAAAATTTCTTGAATGTTTTTATATTTGTTTTTTAATTGATTAACTAAATACTTCTTTAATGATAATTCATATTCATTATTGTTAATTACATTATCTTTATCTTTTTGATAATTATTTTCTAAATATTTTAAATCGACTAATTCCAATAATAAAAACAATGGTATACATGATTCCTTTCCCGCAGTTACTGACTTATCATATTTTTCTACAACGTATCCATTACAATTGGTTAAAAATTTTGAAATATCTAGGTTAAAGCATAACCCAGATTCAGAATATATATATGATAATAAATTAGTAAATCCTAACTCCGGAATCTTATTTACAACTAAATCTGTATATCTATCCATTTATAATGGATAAGCTACCAAATATATATTATTTCCCAAACTGTGTAAATTTATTTCCCTATCAATACTCTCAAAACGAGTTTGAAAACATGTCAAATGAAGAAAAGAAAAATTTTTCCTTTGCTATATTTCCTTTAATAAAACATAGATGGAAGAAATCATATGTAGTAAAAGATAATAAAACGTATAAACTAAGTAATGAGGTCAACAAAACACTTAAAAAAGTTACTAATGTGAATATTCCATCTATTTATCCAACAAAAATAGATGGAGCTTCAAAAGAATACACAATAAATAATATAAAAATTAGTTTTGAATGCTATAGCTATCTAAATTATTCTAAGTTCGATAATATCCACTCATTCGATGAGTATATTTTAAGAGGGTTATTGGAGGGAGGAAATAAACTTAAAATATTTTCCAATAATATAGGTTCTACCAACTCAACCATTGGTATTTTTAATAACACAGAGCCATTTTTTAAAATTAAGTTGGCATCTTTGCAACCAAAATCACAGCAGGAAATATTTAAATCTTGGATATCCCATAAACCAATAATATTGACAGGTGGTACAGGTGTAGGCAAAACGTCACAAGTACCTAAACTAATATTATGGTTTAATTATTTATTTGGTGGGTTTAAAAATTTATCCAATGATTATACATATATAGAAAAACCAATTGTGTTATCATTACCCAGAATTACTTTGGTAAAGTTACATAGTAAAAATTTACTTAACTCTCTAGGTTTCAAATCACTAAACGAATCACCAATTTCACTGAAATTTGGATCTATAACTAAAGATCTAATAAACCTCAATCAAAGGAACTATGGCATTGTTTTTTCAACGCATAGAATAACACTGTCTAAACTTTTTGATTATGGTACTATCATATTAGATGAAGTGCATGAACACGATCAAATAGGAGATATAATAATATCTGTTACTAGAAAATATATTAATAAAATAGAATCATTGTTTTTAATGACAGCTACACTAGAGGACGATATTGATCGTATAAAAGTTTTTTTTGAAAGCCCAATATTTGTTCACATACCAGGTAGTACACTATTTAACATATCTGAAGTTTATATAAAAAATAGTGTAAATATAAAAAATAAATTTAAATATATTGAAGAAGAAAAGAAAAACATGTTAAATGCCATAAAGAAATATACACCGCCAAAAAACTCATCTGGGATAGTTTTTGTTTCATCTATCACACAGTGTGAAAACTATAAGAAGTTTTTATCTAAACATATACCATATGATATACATATAATTCATGGAAAAGTAAAAAATATAGAAGATATATTGTCAGATATTTATTCTACAAATAATACATCAATAATTATATCTACTCCATATTTAGAATCAAGTGTGACGATAAATAATGTAACCCATATTTATGATAGTGGAAGAGTTTACATTCCAGAACCATATGGTGGTAAAGAAATTTTTATTTCTAAATCTATGCGGGAACAAAGAAAAGGTCGTGTTGGTCGAGTGAAGCCCGGAACATATATCTACTTTTACGATATCGCGTATTTAAAACCAATAAAGCGTATAGATTCAGAATTTTTACATAATTACATTTTATATGGAAAAATATTTAACTTATCATTACCAAACGATTTATTTGTACAACCATCTAATTTAGATTTTTTAAACAAAGTAAGTACTTATATAGACTCATTTTGTATCAGTGATGAAAAATGGACATTTATTATGTCTTCTTATTATGTTAATATTTTAGAATATGCAAAAATATACTCTAAGGGTGGAAACGATGCATGTGCGTTGGATATTTTTGAAAGAGAAAATGTTTTAAATGATGATGCCTTTAACGCAATAAAGTTGTTGAATATGAGAGCAAAAATAATAAAGCACAGAAGATATAATAAATTATATGTATTAACGTGTAAATTAATGTTTGGTGTATATTTTGGTAAAACATTTAACATTATACATAATCGACCATTATTTGGTTATATTACTATGATAACAGATAGTACTTTTATAACTGATGATTAAATTAATTTAATTACTATATCTTTTTTCATCGATGAAAAATCTTCTACTATTCCTTTTTCTGAAATAACTAAACAATGATTTTTAAAAATATTTTCCGTCGTGATATACTTTACAACACTATTTGGATAAATAGTAAACATAAACACGTAATACAGATGTTTGTCTAATTTAGTAAAATCAAGAGAATAAATTAATCCTTTTCTTTTTAATACCCACATCATATTCGTTAATGATTCATTCGTGATATTAATTCCTTTAACTAAACCACAAACAAAACTGGAAAATGTTTTTATATGATATTTACTTTTTTTACCAAACACAATTTTATTTTTAAAATGTTTTACGGGTTTTAAAACAGATTCCCTATTTAACAGTATATCTTCAATAGAATTTCCACTTACGAAAAAAGATAAAAGTAAGTACTGATACATGGTAGCAGGTTTTGATTTGCTGTATTTTAAAAAAGTCTCGGATTCCAATATATTTTTAATATCTTCTGATGAAAATTGATGATTTATTTGCACACCATCATTTTTTTTCTTTATTTTATTTTTAAATCCTAATGCTAAATTTACATAGCTTATAAATTCTACATCGTCATATTTTATATAAATCTCATTTTGACTTTGTTTTTTCATTAGTTTTATCCGTGATTTAGTATTAGTATTATTATAAATATCGTTAATAGAAAAGTTATTATTATCCATTACTATTATTTTGTGCATTTGACTATCATTTTTATTAAAAATAACGTTACTAAAATTTGGATATATAGCCACATAGTTTCCAGATGCGATAGAGTTATATATGTTATCTTCTAAAGATTTTAATATTATTGATATATTACAATTAATTTCAGTTATATAATCATAAATGTCGTGTTGAATCCATGGAAACGATAAATATATGTTCATTAAGAAATCATCTCCATAGTTAAACGTTATATTTTTATATGCTTCAAAATCGATAATATAAATACCTTTTATGAATTTTTCGAAATCGTCTTCATTTATGAATGAAATTGAAATGTATAATTGATCACCTATTGTTTCGTAATCAATTAAATGATACATTTCATAAAGAGAAATCATCGATAATATATTATCTAAAGTTGGTTTTATGTTAACCATTACTGTATAAAAAGGTGACGGCATCATAACAAATTTTCCCTTTATCGTTGGTAAATTATTATATGGTATGGTTAGTGGACACGTGGGCAATTTTCCAAATGTTTTTGATAGCAACGATAATGTATATTCGTCTAATGCTTTAACAAATATTACAACATTAGGACCTATAATTCTGTGCATCCTACTTGATAGCATATCATCAATATTGTTAATGTTTTGCAACATTGATATTCTACCTCCGTTATATAAATCTCCGTTTGCTAAAAAAGATAAAGCATCCATACAATGAAATATTTCATTTCTAAAATAATATTCGTTTTCTAATTCTTTTATATGGTCCTTAATTTTAGAAATCGAAAACGTATCTTTTATTTTATTTTCATCAAAAAACCACGATATTAACGTTTCTACCGCATCTATAGTTTTAGATTTTCCTTTTATAGATCGACACCAAAAACTCATATAGCTTCTTGCAGTGGAAGCGTTTGCGACAAATTTTGATGGATCAAAAGTGATAAGAATATGTTCTAACAAATGTGCTATACCTATTATATTACCAATATCATTTTCAAACCCAAAACTAGCAATTCCTAAATATATATCTTTGTTCATGAGTTCATCAATAAATATTCTAACTCCATTTGAAAGAACTATCATTTACTAAGGAGTAAAATAGGAAGTAAAGTCCTTATGTCTTCGTCAAAGTATAAATTAAAAACATATTTTTTGTTATTTGAAATCACTGTTACTGTTCCAGACAAAGAATCGTAATACGTATCTATTTTTGTAAGTGCTACAGATTCGGGTTTTGAAAAAATCATAGAATTGAGTGACGTAGGAAAAGTATTGTTTATATTTTTTATTACGTTATTTTCTTCATTTTTTTGATTAACCGACATAAGGAGTTTATTAGTTGGATAATAACTAAAATAATATAGTAATAATAAAAAAAATATAAAAGAAAGTAAATAAAAAAAATATGCCATTTAGGGATTTGATATTATTCCACCTTTCTAAATTTATTTTAACAAAAAATGAAGAGTCTATTAAAACTACTTTATCTTTATGTAGAGGATTTGATGTAGATTTAGAAGAACTGATGCTTAGTTTTTTAAGTAAAAAATATTTAAAAAAACTTACAAATATAGAATATAATAATTTGTTGCCAGAATTAACGATATCGTTTCCAAATGATATCGTTAACGAATTGATTAAGTTAAGATTATATAAGTTTTATAAAATTATTAAACCTTCTTTAAAACTTAAAAATACAATGAAAGGAATCGTATTTATTAAAAATAGAAATGTGTATATTTTACACACAAATGATGAATTAATCGATTTCTTATTTAAGGAGTATGATCCTTATATATATACGTATGAAATAGATAAACCGGATAACTTATCAGGGTCAAAAATAATTTTGTGTGGATATGACAAAGTTACATTTTATACGTATCTAATAACTAAAATTGTAACAAATCAAACAGTAGATATAGTGGTTACTGATAAATGCATTGAATTATTATTACAACCACAAAACAAACAACTTCTAAAAGATTTATTTAATAACAGCAATGATATGATTAATAAAATATTAAAAAAGATATTTTATTCAGTTATAGATGGGGGCGGCCAAACACCATAAGGAGAATTCTCTATTTCTTCTTTTAGTTTATCTTTTTTAATAAAGCTTTTATCTATTACTGCTAGATTAAAAAACTTAGATATATTAACATATGCCATTTGTCCGGTTTCGGGATTATATTTAAATAATGAAGCAGCCTCTGAGCTTAAATATTTAAAGAAGTTATTAATAAACGCAACCCCTTCATCTATTTCAAACTCTTTAATTTGACCATTTTTGGAAAAAAATGATAATATATTTATACGTAATATATCATATTCATCTTCTAAAATTTTAATAGCATCACTTATAGATTCACAAACTCCACACAGAGGTTTTCCAAACAATATCAACGTTTTCTTCATTTATGGGTATCAAAAATTTAAAAACTGTACTGGTAGAAATTGGCGCATTACACCAAATTAAAAATGTACAAGAAAATATAATAAATGGAATATTTGTAGACACAATGAGTTTTTTCGTATCTATAGCACATTGTGTCAATAATTTAGATGATCTGTATGAAAGTTTTATGTTATACATATCCCAATGGAAGAAACAAGGAAAAATTACACTGTTTGTTGATAGGGGAGTTATTCCAATAAAAGAATCACTTAGAGAAAAAAGAAGAAATGCATCAAGAAATACATCAAAAAAAAACGTGTTAGAAATAGAAAAACTTAATAACTTAATAAAAAATTTAAACGTTGACGATATGATGTATGACGAAATAAAAACAGATTTAGAGTTAAAAATTAAAAAGCTTGAATTTAATAATTATTTAGCTAATCATGTACAATTAAAAGATGCTTTAGATAATTCATTAAAACTACTAGGAAGCGATGTAACAATCATATACTGTGATGGTATCGATGCAGAATTTGTCATGTGTCAAGAAGCGAAAAAAATAGCATATATGACCGAAAAATGGCCATTAATGATTAGTACTGATCAAGATACTTTGCTTTTTTCATCATGCGACGATCTTCCAAAAAATATAAGAACAATGAATCAAATTTATTCGTTTATACCTTGCGCAAAAACTAGATATCTTTCTAAGTTAGTTGCATTAACTAACGGATGCGATTATTTTCAAGGGCTTTATGGATTTTCTATAACTGCAAAATCATTAAAATCTATTAAATTGTTTGATGATTTTACTATTGATAATGTGATAAAAAGCTTAATCATTAGAAATTATTCAAGGAAAGAAACAGACAGATATATAAATGCCGAAAAAATCATACATTTTATAAATAAATATTCGTGCTTAAATGAATCTATATATAATGAAGTCCCCCCTGAAAGTATAACAGTGCAAGAATTTATTTTTTCAGCATTATATCATAAATGGAAAAAGTTTGACGATAGTTTACTAAAAGGAATTTCATTATGTTGTTCACTTATATGTGTTCTCAAACCTAAAAAAGATATAAAAAGGAATGAAATTAGTAAATTATGTAATATAATTAATAATGATTCAAATAAAAAAAGTAGTCTAAATAATATCAAATCTGTCATAGATATATTTGGCTATGATCTAAACAAAAATAAAAATATAGTTTATGGAATATCTAAGTTAAAAAAAATTATGCTTTGCTATGACGACCTGTTTTATTTTAACAATGAAAACATAATTAGAAATAACTTAAAAAAAAATGGTATTATAAATATATCATAAATGGTTTTTCAATTAGTTTGTTCAACATGTGGGCGCGACATATCAGAAGAACGTTATGCGTTATTAATAAAAGAAGTAAATATAAAAAAAGTTTTATCTAGAGTTAAAAATAGTTGTTGTAGACTAAAGTTATCAACACAGATAGAGCCTCAAAGAAATTTAACCGTGCAGCCTCTTTTAGATATTAATTAATGGATCCTGCAACTTTTATAAAAAATTATGCGCCTAAAGGCGCTGTTATTTTTATTAATTATGAATTTTCCTTAACTGAATATTTTAATCCATCAGAAGATAAACACGCTGCTATATATATCGGATCCGACTGTACATCTAACCATGTTAAAATGTTTTTATCGTGTAGCAAATTCGATGAAAAAATGGCAATAGAAGCATCGTATAAAAATGGAGTTAGATTAGTTAATATCGATGATTTAATAAAGGGAGCCATAAGTGTTAAAGTTCTTACATTAAATGATGTTATTAGTCAATATAAAATGGGATTGGCTGCGGATAACGCAATGAGCTTCTTAGGAATGCCATATGGTTTTGGAAATGATAATATTTACTGTTTTAAATTAGTAGCTGACTCATACAAATTTTTAGGCGTTACTCTTCCCACTTATAATATTTTAGGAAAACAAATATTTTTAAGCCAAAGTTTCGTAAATAATAAACAATGGAAAATAGTATACTCGTTTTCAAAACCGACAAACTAATATAATTATAAATTTACTTTACAGAATTTAATCCAGGAACTGTTATTGTTTTAAATGGTGAGTTTATATTAAGTATAATACCACCACTAGTTTTATTATCCCCATTTTTCTTTTTAATATTATTAGCCATAACACGCATTATATATAAATATTCTAATATTGTAAATTTTTCCATATCCGTTACAGATAATGGAATTTGGTTTGAAATAAATGCGTTTTCTGTTATTGGACTCGACACGGCACCATCTAAAACATGACCCTTTGAATGTTTTTCAGCCTCTTTTAGTAAATCTATTATTTTACCAGAATAATTTTCATTATTGTTTTTTAGAACTTGTTTTACGTTATTGTCTTCATCATTTTGCGCGCATGTATTTTTAACTATTTCGTTAATTTTTAATCCTGCAACGTATTCTGCAAAATTATTAGATGTATTAATTCCTTTTTCAGATAAAAACTTATACAAGTTAGGTATTATATCTGTTTTAATTGGTAATTCCATTATTTTGTTTACTTCAACACTGTAATCAGTTGTTGATGTAGATCCTGCAAAAAATATTGGCGTTGATAATGTTCCTATAAGTGGCTCTTTTATACCCATGATAATAGGAAATATTTTGTACTGTACTAAATTTATTAATCCATATTTCATCTCTAAAAGATCTGCAAGTTCTTGCGCCGGAGTTTTTTGTGATGAAAAAAGATAAGTTTCAATGCTTTCAACCATTTCGGCGGCATATAGTAATTTATTAGAAAGGACTAACGTTATTAATGTAGCCAACGTGGTTACTATTGTCGGCATACATGTTACAAAAAAAGATTTAGAATGTGTAAATCTATAAAGGCTATTATACAATCGCCAAAATTCGCCTATGTTATATACATAAACAGAATGTGCCTTTAAATTGTTTAAAATATCCTTTAAATGATTATTATCATTAATTGATATATCTTTCTCAATGCAATTATAAATACTATTAATGATATTTTCTTTATATGATGAATTATTACAATAACATAAATGTTTTGATTTATTTTCTAAGTATTCATTATTGACCGATAATCCTTTAACTACAGATTTAATTATTGATTTTGATATAATTGAATAAATCGATGATTGTTTTGGTTCTATCATTTAACTTTAAATAATTTACAAAAAATAAAATGAGTCTTCGTATAAAAATAGATAAATTACGACAAATAGTTGCGTATTTTTCTGAATTTAGTGAAGAAGTTTCAATAAATGTTGATTCAAATAGTAACTTAATGTATATTTTTGCAGCACTTGGGGGATCTGTTAATATTTGGGCAATCATTCCATTAAGTGCTTCAGTTTTTTATGATGGTAAAAACAACCAAGTATTTAATATTCCAGTATCAAAAGTAAAATCGTGTCTTTGTAGTTTTCATAACGATGCGATAATCGAAATAGAACCAGATATTGAAAATAACTTAATAAAATTATCCAGTTATCATGTTGTAAGCGTTGATTGTAATAAAGAATTAATGCCTATACGAACTGATACAACTATTTCTTTAAAAATAGATCAAAAAAAGTCTTACATTTTTAACTTTCACAAATACGAAGAAAAATGTTGTGGTAGAACAGTAATTCATTTAGAACTATTGTTAGGTTTTATAAAATGTATAAATCAATATCAACATTTATCAATTTTATTTAAAAACGACAATATAATTTTAAGAACACCTGGGAATTTGGATACGTTTTCTAGAGAATATTCTATGACTGAATGCTCTCAAGAACTTCAAAAATTTTCATTTAAAATGGCAATATCATCACTTAACAAACTAAGAGGATTCAAAAAAAGAGTTAATGTGTTCGAAACAAGAATCGTAATGGATAAAGACGACAACGTATTAGGTATGTTATTTAGTGATAGAATACCATCTTTTCGCATTAATATTTTTATGGCATTCCACGATTAAGTTTAAATAAATAACTAACAAATAAAATGGGTTCTTCTTTTACAGTTCCTGAAAAAATTAAAATAAGCAACAGGCCAAAAAAAGAAACAATGGAAATGAGATTAAATGTTGATAATATGTATGAACAAATAAAAACATTAAATCAAATCAATGATGAGTTATATATAGGCATAATAAACGAAGAAAAAAAGAAACAACTTATTAAGCAATTTCCTGAGTTTTTATTTATTGAATCAGGTCCAGGAAATCTACATAAAGTAATAAGATCGAAGTATAACAATGATAAAAAATATTGTTGTAAAAGTATGAATCTTTTTAGTTATTGGCAAGACAATAATGGAAACATTACTGATTTTTATAAACCAAATTCTATTTTGAGTAGCTGCGATCCGGATATACAAAACAGTGGGTTTTGCGACGACACACTTTTAAGTTGGTGCGATACCGCCACACAATCAAACTCAAAATTATGTTACGACTGGATAAATTCTGCATTAAATAGAAATAATTCATCGTCTGAAAAATTGATAAATGGACTAATAACAACGTGTTCTAATAATGCAAATACGCCAATATGTAATATTTTTTTACATTGTTTAAGAGTAAAAAACACTGAGACATTTGATAATGTAATAGATTATATTTTATACTCTCAAAGTGATGATTTTAAACGAACATATATGAAATGTAGTTATCCATCAAATAAAATTATAAAAGAATCCCTAAAATTTTTTGAAGCGAGAGAGTGTTGGGACCCAAATTGTTCAGAATCTAATTTAAACTTTTTATTGACAAAAAATTATAAAAATCTTGGTTTGTGTAAAATAGATAGATGTAATATTAGCATTAACAATTTATTTTTTGATGAAGGATCTAGTGTACGGATGTCTTGCAACGACGAATCTATAAAATCTAAATTTCCTATTAATAAAGAAAAAGTAATTTTACATAATGTAGATAATTCATTTCAGTTAAAAATACATCTTTTAACAATATTATCTTTATTTGTTATATGGGTATTAATTGTAGCTATTTAAATGGGAGCAGCCGCAAGTATACAAACTACAGTAAATACGTTAAATGAAAAAATAAGTAGTAAATTGGAACAAACTGCTGAAGCCACTGCGGAAGCAAAATGCGATATAGAAATTGGTAGTATTGTATTTAGACAAAATAAGGGTTGTAATGTTACTGTAAAAAACTTGTGTTCGTCTAAAGCAGAATCTCAATTAGATGCTATATTAAAAGCAGCAACAGAAACATATGATTCACTTACTCCTGATCAAAAAGCATATGTTCCAGGATTGATGACAGCGGCCTTAAATATCCAAACAAGTGTTAATACTGTGGTTAAAGATTTTGAAACGTATGTAAAACAAAAATGTACATCGAAATCGGTTATTGATAATAAATTAAAGATTCATAATATTTTTATAGATGAATGTGCTGCACCAACCGGAACAACGACAAACTTTGAATTTATTAATTCTGGAACCAGTCAGGGAATATGTGCAATAAAAACGTTAATGGATGTAACCACAAAGGCGAGTACAAAATTTTCCCCTAGTCAAAGTTCGGGATACGGATATCAATTTTATATAATTGCGGCAGTTGTTGTAATATTATCGATGGTTTTTTTATACTATGTAAAAAAAATGCTATTTACTTCTACAAAGGATAAAATAAAAATTATTTTAGCTAACAAACCAGAAGTTCACTGGACATCTTATTTGGATACCTTTTTTAGTAATACACCAACAATCATAGAAAAATAAAAGTGAAAAAAGAAAAGAAAAAATTAATTAGGATAAAATGGCGGAAATATTAACAAATAAACTAAAAAGTATAGAGAATGAAAATAAATACAACGAAAAATTGATAGAATGCATAATTCAAGAAATAGAAAAACAGCAATATTTTATTTTAAAACCCATAGTGCGATTTATCATAGATGTAATGATAATCGTAATTGTTTTAAATGATTTAACAATTAGGTTATTGAAGCGAAACTATAAAATTTTATTTTCTATCGTATTATTATCTATACTTTATGATGCTTTTGCACATAGCGATGTTTCGTCAGATATATAAAATATCACATCAAATAATTATTGATGTTAATTGTGATCTTATAAAATATTCATTAGCGAGAAAAACATCTTGTATGTTAAAATCATCTATTTCTAAAATGTATCCGCCATCTAAAGAAAACAAAACTCTTACATTGTCACTAATTGAAACATCCGTTAATGTTATTCCAGATATGTACATATCATCTCTATATACCAAAACTATGGTTCCTGGTTCAACGAATCTATTTATGTGATTGGTAGTAACGGTTCTCTTGCGTATTTTAAACAAATCTGTTCCATCTTTAGATAATAATGAAATATGATTTTTTATTAACCACTCAAAAAAGTAGTTTGAGGTTTCATAACTTTTCCTCATTGTTGTTTCTAAACAATGTAAAAAGTTTAAATAACAAGATTGTGGTAGGTTTACTTTATAAGGTCTAGTGCATAAAGATAATATTACCAACTTGTCAGTGCATATTTCAAAGACTATTTGATTAACTGAAATAAAAAAAGTAGTTTTTGGAATATCTACAATGTCAAATTCGAAATTATCTACGTATATTTCTCTTTTATGCATTGAATATAAAATTACAGCAATTTGGAATAATATACTTTCGTGTAGTAAGGACGACGTCACTACCAACTTTTCAACCTTTGTATTTTTGAATTGTTCAAAGTATAATATTTTACCAGATGAAAAACTTTTGTAACCTCTAAGTAATGGAAACCCTATTGATTTTTTCGATCTAACTAATGATGATAACTCAATCCAACATTCGGTTTCTTCTTTGCATGCTTGTTTTAATATACAGTTAATCTTTACTAAATTACATTCTAAATCTAATGATTCTTTTTCGTGTAACATTAATCTATTTGAAATAAAATCATCGTGATAACAATTATACTTAGTATCCGAATCTTTTTTAAAGTTTTTATTTTTTTTATCATTTTCATAATGTTTATGTTGTTTATTACTATTCATTTATCACAAAAAAAACTTATCTAAATGAGTCTCATTTTAGAAAACCTGATTGATGAAGAAACAATATTTTATGCGGGCAGTATAACCGATTATACAGAAATGGAATTAATAATCGCAGGTGCTAAAATAAAATATCCTAGATCGTTTTTATCTATATTTAACATAATACCAAGAAATATGACTAAATATGAAATGGATTTAGTTCAAACAGAAAATATCACAGGTGCAGTATTTACAACAGCTTTTAATATAAAAAAGAATTTGGGTATCGTAGATGAAAAACTTACTATAGAATCCATAGAAAATTATTATTTAGATCCCAACAATGATGTTTTGACTCTTATGATTAATAATACTTCATTAGATAATGTAACAACAAGAAAAAAAAGATCTAAAAGAAATAAAAATCCTGTACTTTTTAGGCAAGGATCCATTCCATTATTTTTTATTTTTGAATCCAGAAAAAAGGTAGAAATTTATAAAGAAAATGTTGATAAAGCATCATCAGATAATTCATATATGAAAATTGATGACAATATTGCTGTAGCAAGCAAGTATGCTAATATGTCGTTATTAGATGTTCATTCACCATCCGCATCAATGAAACTTAATGGAGTTTATGGTTTTACGTATAAAAATGAACTAAGAAAACTTAGTTCAGATAAAGAAATTGATGAATATAGCAACAAGCCATTACAAGAACCAGTTAGATTAAATGACTTTATTGGACTGTTTGATTGTGTAAAGAAAAATATACCACTAACAAATATTCCAATTATGGAATAAAATATCATCAGTTAAATGGAGAAAAATAACTCAAAAAACATTTATTTTACTCCTGTATTTATAGAACCTACTATAAAACATTCCCTTTTGCAATCTTACAAATATACATATATCATAATATTTGAAATTATAACAGTGATGGTATTATTATTTTTATTTTTTAAGTCAGAAATCCACATGTTGTTTAAATTCAAACAACAAAAAGTTATCAGTCCGATAGACAAATTTTCAAAAACCACTTTATACTGTAAAGAAAATAAGCTTTTTATTAGTGGGTTACCTAATACTATGTACTCAAAGGAAGCACTATCATTAAATAGACAACCGATAACATATAAATATTGTAATGATCTTTTACAATCAATAAATGGATCACAGCAAGTATTTATTAACGATATTCTTAGAAAATGATGACTCCTTTTTTAAATACTTATCAGAACAAGATGATGAAACAGCTATGTCTGATATCGAAACTATTGTAACATATTTAAATTTTTTATTGTCATTGTTAATTAGATCAAAGGATAAATTAGAGTCGATAGGTTATTATTATGAACCACTGTCTGAAGAATGTAAAACATTAGTTGATTTTTCCAATATGAAAAATTTTAGGATATTATTTAATAAGATTCCTATAAATATACTAAATAAACAAATAACTGTAAATAAAGGGTACTTATCAGATTTTGTTACGACATTGATGAGATTAAAAAAAGAACTTTTTTTAGAATCACCAGAGCCGATAACATATATAGACCCTAGAAAAGATCCAACATTTTTAAACATTTTATCAATATTGCACGAAAATAATTGAACAAATATTTTTTAAAAAAAAATGGACTATGGATATATACATTTAATTATAGGACCTATGTTTTCTGGCAAAAGTACTGAATTGATAAGAATAGTTAAAAGGTACCAAATAGCGCAGTATAAATGCTGTGTAGTAAAATACTTAAAAGATATCCGATATGGTAATTCTGTGTATACACATGATAATAACCATGTATCTGCCATATCAACAACTTTATTATATGACGTCGTTGATAAAATTATGAATTTCGACATTATAGGTATAGATGAAGGGCAATTTTTTAAAGATATTGTATCTTTTTCTGAAAATATGGCAAATATGGGAAAGATAATTATAATAGCTGCACTAGATAGCACGTTTCAACGAAAAGAATTTAATGATATATTGAAATTGATACCGTTATCTGAAAAAGTAACAAAATTAAACGCTGTATGTATGGAATGTTATAAAGACGCCGCATTTTCTAAGAGGATCACTAAAGAAAAGGAAATAGAACTCATCGGGGGTAAAGAAAAATATAAATCTGTTTGTAGGAAATGTTATTTTTTAGAATAATAAATATTAATGAAAAAAAATCAAAAAAAAGTGATCTATTTACTTATTAAACTATATAGTTAATAAGTAAAATGGGTATCAGACACGAGTTAGATATTTTGCTTGTTTCTGAAAATCTCGCACTGAAGAATGTTGAACTTCTTAAAGGTGATAGTTATGGATGTACTATTAATATAAAAGTTAATCAACAAAAAAAATTGGATTTTATTATTATATTACGGCCCGATTGGACAGAGGTAAGGAATGTTAAAAAAATAAATATGGTATGTAACGGTGTTGTTATTGATACAACACTAATTAAAAAATCTTTTTACGAAGAAGTATATTCATCATCTGTAACAGTTTTTCAAAATACTACCGTTGAATTTTTTAGTGATACTAGTAAGAAATATAAAGAAGAATATCCCATTGTTAACATAAACACCATAAAGCGTTATTACGAAATAAAAGATTCAAAAATGACATGTATAAATTTTGAATCACCTATAAGTGATTATGATCAAGTAAATTATTTAAAAGATTACATAAATATAAGTGATGATTATTATCTGTATGACGCATGCGATGATTGTATCATTAGTAGCGATGATGATGACGATAATGATAATGCGGACGATGACGATGAGGACGACGAGGTTAATGATATAGAGGATGACTATGAATGATTTTTTTGAAAACTAATGATTAATATATTTAAATATTAACAAAATTTACTTTAAAAATGGAAGCAGTATCTATGGATAAACCATTTATGTATTTTGACGAAATAGATAACGAATTAGAATATGACCCTAAAACTTCCGAAGAAAAGCCAAAAAAATTACCATATCAAGGTCAATTAAAATTACTTCTTTGTGAATTATTTTTTCTGAGTAAGTTACAAAGACATGGAATATTGGATGGATGCACAATAGTATATGTTGGATCAGCTCCAGGAACACATATTAAATACTTAAGAGATCATTTTTTATCTATGGGATTAGTTATAAGATGGATATTGATAGATGGACGACAACATGACACAATTTTAAATGGGCTCAGAGACGTGACGTTAATAACTAAGTTTGTAGATGAAAGTTATATAAGAGTTTTAAAAAAACAGTTATATCAATCAAAAATAGTTCTTATTTCTGATGTTAGATCCAAAAGAGGAGGAAACGAACCTAGCACATTTGATTTATTAAGTAATTACGCATTACAAAATATAATGGTAAGTATATTAAAACCAGCAGCATCTAGTTTAAAATGGCGATGTCCATTCCCTGATCAATGGGTAAAAGATTTCTATATTCCTCACGGAAATGAAATGCTTCAACCATTTGCGCCTAAATATTCTGCAGAAATGAGGTTAATAAGTATTTATAGCGGTAATCCTATAAAGCTTAGATGCATCACAAAGGATGATTCTATAAAATATGAAAAAAAGATGTTTTATTTTAATAAAATAATAAGGAATAGAATTATTATAAACTTTGATTATTCAAATCAAGAATATGACTTTTATCACATGTATAATATGTTAAAAACTGTATATTCTAATAAAGATTTTTCATCTATAAAATCAAAAGTTTTATTTTTTCACCATTCAATTTTCAAGTTTTTAAAAATACCTATATTCAACACAGAAAAAATAAACTATGAACCCACACAACGTAAAATACTTAGCAAAAATATTATGTTTAAAAACCGAAATATTAAAAAATCCATACGCTATAATAAGTAAAGATGTTGTTTTACGATATAATACAACTATAAAATATGGAGATCTTGTAACATATATAACAGTACAACATAAAATAGATGCAACTAAAACTCTTTTTCAAGTTTTTAATGAATCTTCTGTTACATATTCACCTTTAGAAAATGATTACGGATTCCCGATAATAATAACATCTTTTCTACAAACGGGTCATAATAAATTTCCTATCAACTTCTTATATATAGATATCGTTGCATCTGATTTATTTCCAAAATTTGTTAGATTAACTGATGAAGAAATAGTTACTGTACAAAGTGTTTTACAAATAGGCGATAGTAAAGAAAGCTTAAAGCTTCCAAAAATGTTAGAAACAGAGATAGCTGCAAAAATATTATTTCACAAAGATATCCCACTTAAGATAATAAGATTTTTTAGAAACAATATAATCACGGGTATTGAAATATCTGATAGAGCAGTAATAACTGTATTAGATTAATAATATTATGTAATCAGTATTAGAGACGTTATTATAAATAAAGATATTGGCAACCATATGGGATGTATGATAGGTTCTTCTATTTCTTGATTTAAATATTTGGTTCGAATATATTCATTTGTAAAAGATGAATTAGTACCACAAACATTTTTAACATCAATATATCCATTTTTTATTTTAATATTACCTAATGTTACTCTACAATCTGAAATATTACATAGTGATATGTTCTTTTTAAGCGATTTAACAATAAGCGCATCTGATCTTTTACATGGCTCATACCAACAATAGTATGGTAATCTTGTTTCTTCTCCTATTTTTATTATACTCGAATCAGGATTTAAACACCTACATCTTATATCGTTTTCGTTTTTATCACAGAAAGAATATATATCTTCATCTAACATTTTTATAATAAAAGAAAAAAAAAGAAAAAAAGAAAAAATCAGTTAATATATTGTATTTGTATTAATAAAAAACTAGTAAATAAAAATGGCAGTAATATCTAAAGTAACATATAGTTTATATAATCAGGAAGAAATTAATGCTACTGATATATTTATTAATCATGTCAAAAACGATGATGATGTTGGAACGGTTAAAGATGGCAGGTTAGGTGCCATGGATGGAGCATTATGTAAAACTTGTGGTAAAACAGAATTGCAATGTTTTGGACATTGGGGAAAAGTAAGACTTTATGAGACACATATAATAAAACCAGAATACATAGGTGAAGTAATAAGAATTCTTAACCATATATGTATAAGATGTGGATTTTTAAGATCTAGAGAACCATACATAGAAGACGTAACCAAAATGTCATCGCATGCACTTAGAAAGTTAAAGGATAAGATCCTTTCTAAAAAAAAATCTTGTTGGAATAGTAAATGTATGCAACAATATCAAAAAATAACATTCTCAAAAAAAAAAGTATGTTTCGTAAACAAATCAGACGATATCACAATCCCTAATGCGTTGATATATCAAAAAATAACGTCAATTTATAAACGTTTTTGGCCATTGTTAGAAATTCATCAAAAACCGGAAAATTTATTTTATAAAAACTTTTTTCCTGTACCACCGTTGATAATTAGGCCAGCTATTAGTTTTTGGATAGATAGTATACCAAAAGAAACAAATGAACTAACTTACTTATTAGGTATGATTGTAAAGTACTGTAATATGAATGCGGACGAACAGGTGATACAAAAAGCAATAATAGAATATGATGATATTAAAATTATATCAAATAATACTACTAGTATAAACTTATCATACATAACATCTGGAAAGAATAATATGATAAGAAGCTATATTGTTGCAAGACGAAAAGATCAAACTGCTAGATCTGTTATTGGACCTGATATCACATTAACAATTAATGAAGTAGGTGTTCCATTTTATATAAGGAATACTCTAACTGAAAAAATATTTGTAAATCCATTCACCACGGATGAGGTACAGAGATTATTTACAAATAATGAGATTAAGTTTTACTTTAATAAACGACTAAATCAATTAACTAGAATAAAGCAAGGAAAATTTATTAAAAACAAAATACATATGCTTCCTGGGGATTGGGTAGAAGTACCATTAAAAGAAAACACGAGCATAATTTTTGGAAGGCAACCTTCTTTACATAGATACAATGTTATTGCTTCTACAGTTAAATATATTGAAGGTGATACAATAAAAATACCACCCGGTATAGCAAACTCACAAAATGCTGATTTTGACGGTGACGAAGAATGGATGATACTTGAACAAAACCCCAAAGCTGTTATAGAGCAAAGTATTTTAATGTATCCAACTACATTGTTAAAACATGACGTTAATGGATCTCCTGTATATGGTTCTATACAAGATGAAATAGTGGCAGCGTATTCCCTTTTTAGGATGGAAAATTTAACACTTGATGAGGTTATGAATCTATTAGGAAAGTATGGTATTAATTTTTCATCCAATGGAAAAACAGTATTCTCAGGAAAGGATATACTTGAATTTCTTATAAATGATAAAGTTAACTATCCTGGCATTTTAAGTAATGGTAGGATAGTCGCAAATGATATTATAAGCGATTTTATAGTTGCGATGCGTGGCATGTCACTTGCCGGGTTTATATCTGACTATAAATCTAACGTAGAAGGTATTGAATTTATCATAAAATCATCTTACGTCTTTAAGAGGTATTTAAGTATTTATGGATTTGGGGTCACATTTAAAGAATTACGCCCCAATTCTGATTTTACAAACAAATTAGAAGCAATAAACTCAGAAAAAATTGAGTTAATTAAAAATTCATATAAAAAATATCTAACAGATGTAAAAGATGGTAAAATATTACCTCTTTCAAAATCTATGGAATCTGACGCTGTAGAATCAATGCTTTCCAACTTAACAAACCTTAATATAAAAGAAATTGAAGAATATATGAAGACAACATTAATCAATAATCCAGATAATAATTTACTAAAGATGGCAAAGGCTGGTTATAAAGTAAACCCAACTGAGTTAATGTATATATTAGGAACTTACGGACAACAAAGGATTGATGGTGAGCCTACAGAAACAAGGGTGATGGGAAGGGTGTTACCGTATTATTTACCTGATTCAAAAGATCCAGAGGGGAGAGGATATATATTAAACTCATTGACGCAAGGGTTAACCGGATCACAATATTATTTTTCCATGTTAGTAGCACGATCACAATCAACAGATATTGTTTGTGAAACATCTAGAACAGGAACGTTGGCACGTAAAATTATAAAAAAGATGGAAGATATGGTTGTGGATGGATACGGGCAAGTTATTTACGGTAACATTTTGATAAAGTATGCGGCTAATTATACAAAAATTTCAGCATCTGTTTGTAAACCAATTGATTTAATATTTCCACGAGAGTCGATGGTTTGGTATTTTGAAATAAGCTCCATTTGGGAAAAAATCAAAGATGGTTTTATATATTCACAAAAACAAAAAATATCAAAAAAAACATTATCACCATTTAATTTTTTGGTGTTTTTAGAACCATCTGATGAAAAACATGCAATAAGTTCTAAAGATTTATACGACATGATACAAAATGTAATCGATGACGTTAGTGAAAAATATTTCTTTACTATATCGAGTATAGATTTTATAAAGTATGTATTTCTAACTCACTTAAATCCTTCAATAATCAGAATAACAAAAGAAACTGCTACAACAATTTTTAAAAAATTTTATGAAAAGTTAAATTATTCATTAGGTGGTGGGACCCCTATAGGTATTATTGCTGCTCAGGTATTATCTGAAAAGTTTACTCAACAAGCGTTATCTAGTTTTCACACCACTGAAAAGAGTGGAGCTATAAAACATAAACTTGGTTTTAATGAATTTAATAACTTAACAAATTTAAGCAAAAATAAAACAGAAATTATTACATTAATATCTGAAGATATCGAAAAACTTCAAAGTGTTAAAATAAACTTTGAGTTTGTATGTTTAGGAGAATTGAATCCAGATATTGTATTAAATAATGATAATGAAAAATACATAATTGATATCACTATCAATCGTATTTATATAAAACGAGCAGATATAACTGAATTAGTCGTTGAACATATGATAGAAAGATTTGTATCATTCAGTGTATTAGTTAAGGAATGGGGATTAGAAACACATATAGAAGATAATAATAATATAAAGTTTACAATACTTGTAAAATTTATCGAACCTGAAGAATTATCACTAAATAAGTTTATGATGGTATTGCCAGGAGCTGCAAATAAAGGGAAAATTAGTAAATTTAAGATCCCTATAAGTAATTACAATTACTACGATGATCATAATAAAAGTAGGAAAATGAATAAAATGACAGTTGAACTGATGAATTTGAAAGAGTTGGGAACCTTTAATTTAGAAAATGTAATAGTATATCCTGGTATATGGAATACATATGATATATTTGGTATAGAATCAGCCAGATCATACTTATGCGAAGCTTTATTAAATACATACGGTGAAGGATTTGATTATTTGTATCAACCATGTGACTTATTGGCTAGCTTAATTTGTATGAGTTACGAACCCGAATCAGTTAATAAGTTTAAGTTTGGTGTTACAAGTTCCCTTAAAAAGGCTACGTTTGGAGATAACAAAGCTCTATTAAATGCTGCATTACATAAAAAGACTGAACCTGTTAATGATAACAGCAGTTGTCATTTCTTTAGCAAGGTTCCAAAAATAGGAACAGGGTACTACCGTTATTTTGTTAACCTAGAATTACTAACTAGATTGGAAAAGAAACTTTCTGTTACAATATCTGATAGAAAGGTTGAAGAATTAATCGAAAAATCCGACGAGTTTTAACTTTTTATTCATTAATAATATACTTATCAATTAGTTGTTTTCTAAAAGAGGGATTTTCTATAAATGCGCCTCTTTTTTCTCTCATAGAGTGGTATATATATAAAAAATATATAAACGCAGGTATATCTTTACTTCTCTTTGACATTAAATATGCCATTATCATCGCACCACTTCTATTAACGCCGGCAACACAATGTACTAACACAGGATAATGTTCTTCATCACATTTTGATAAAAAATTTGTAACATAATCAAAATGATCATTTAAATTTGTTTTTTCGTCATCAATAAGAGGCATATGAATAATATTTATACGTGAATCATTAAAACAATATTTTTCTGTAGTAAGATTTAAAATATATTTAAAATTTACATTTGAAGATATTGCATTAATTGCATCATCATAATTTCCTAAATATACATAATCCGTTACTCTCATCATCTTTGTCGGTGCCTTCGCTTTGGGTAGAGAACCTGTAGACTTTAGTAAAACGTTTTCGTAAAGACTTTTTTTATCCATTTATAATGGCTACAAATGGATAAAACAACATTAAATGTTAATGGTGTTGAATTAGAATACGCTAGAGAAAAAGAAAATAAAACAATACAATCGGCTAAAACATCTACTTTATGTTTCTTTATGTTAATTTTAGGTATTAGTATACTATTACTTTGGTTTCAAGTATCTGATAATTCTATTTTCTCAGAATTAATGAAGTATATACGGATAAAAAAATCTATAAGAGGGTGGAGACCTTTAGTTGAAACCAAAACTAACTTAGAAAGTGATAGAGGAAAAATGTTATCAATGGGAAGAGATGAGTATTTTTCTTTTAATTGTGTAGATTTTGGTTCTTATTTTGTTCCTATTAGATTAGATAGAAAAACGTTTTTACCACAGGCTATTAGAAGGGGAAAAGGTGATGGATGGATGGTACAAAAAGCAGATAAAATAGATGTATCCGCGCAACAATTTTGTCAATATTTAATTAAGCATAAATCAGAAAACATAATAACGTGCGGAACCCATATGATTAACGAAATAGGATATAGTGGCTATTTTACAAACTCTCATTGGTGTTCGGATTTTCATAATCTTTTAAACTAAATTATATACGTAAATAACATACCTGCTAAAAACCATAGTAATTTAGAATTCAAATCAAAAATTATCATTATTATAATAAATAAAATAATAAGTGCTCCTATTATACTAATATCAAATATACCAAAAAATGAAACCAATGGATGGGATACATAGTAAGAAAAATCAGGAAATCTATGAGCCATCCATTTTCCAACTCTATTCCATATACCGTTTTTCATTTCGTTAGCTCTTTTTTTTGACAAATACTTAGTATTGTGAAGTATATACTTAGATGAGTCATTTAAAACTTGTCTATTGAGTTTTAATTCTTTTTCCAACTTATACATTTCAAAACTTAAACTGGTAGAAATACCTTTATTTTTTATAATTTCGTTTATTATTTTTATGGCAGACGATAATCTAATTACATATGCGGATAAGGTAAAATCATATCCCCCTGTGTACGAATACATAAAATTATTATTTTCTTGATTGAACAATATTCTAGAATTACTATTATGAAAAGTTTCACGTAATTGGAAAATGTCTATATTTTTTTCTTGCATTTCAATTATAATGTTATGTATTGTTATTAAATCTTTTAACGTGTTATCATCTTCTATAACAACAATAAACTTATTCTCAGAATTTTTTATATCCGCATCGGCATACGATTTCCATAAACTAAAGTGTTTAACAATAGAACTTTTTGCCTCTTTTGTACAAAGAGCATTACATAATCCAGAAAAATATTCTGTAAAATTTTCAACACCTCCTGATTTTTCTACCTTTTCCCATATAAGGAACTTATATGATAAACTAATATCTTTTTTATCTTTAAAAAAAAAATTTACATCTGAATTTTTAAAATCTTTTACTGTGTCGACTTTTTTATAAAATATATCATTATCACTTTTTAATTCTGGAACTACATCTGAAATTTCGCGACCAACGATTGGTATAACATATAATGGAATATCTGCCATTTTGATAATTAGTTATCTAAAGCACTATTTAGTTATTAAAATTAAAGAAGTATAGCTCTCTAATTTTAGTTATGTTTTCATCTATCCAAATAGAAACATCATTTACGTATCTTGATAAAAATCTATAAAATGTATACTCTAATTTATCTCTATTAAGCGAATCGCTAAAATATACAAAAACATCACTTTCTGGAAGCAAGTGATAACGTAATAAGTTGTAACAATTTACTTTACTCTGCACATGATATTTATCTACAAGTTCGTCATCAAAAGGAAACGGATCTCCAAATCTAAATACGTATAATGATGGGATAGAATAGTAATATTTTAAAACATTATTTATAAATAAGTTTTTTAACGCGATTATCATTTTTTTCCTTTCTATTTCTATTTTTAAATGCGTCGGAAAAATAACTAATTTAGTAGCGTTTGTGTCGTTGTATCTAATCAAAACTTTTAATTCATCATCAGATGTTATCTTTTTAAATTGTTCCCACATTTCCTTCGTATCGTTTAAAATAGGAATAGGGTGAGCAATAATATTACGCTTTTTAATTTTGTTTTCGATAACCGGCATATTGTTCACGATAAGTGTGTAACTTTCAAAAAATTTTAATGCTGCAAGTGGCGAATCCAATGTTTTAACTTCAACGTCATAATTTGGCGCAACTAAAGATCTTTCTATAGAAACTAATTTTCTAAGTTCGATTATTATTCTTTGAAGATGAAGATCTAATTCTTCTGGCATGATGGATGTATAGACATCAGTAAATAATATTATTGTATCTAAAAACCCTTTTTCACAAATTTTAGTTTTTAACAAAAAATCATATATTATAACCGAAATTGCATATTTTAAAGATGACTCATCAACCTTTTTTTTCTTAGAATTCATGTACGAAACAATAAAATCAGCACTACTGTTTAGTATAATCACTAAAACGACGATATAGTTTAAGTTTAATATTTTTGATGAATAAAATAATTCAGTAGATGATACATGAATATCAAATAAATTAGCGGATAATCTGAGGAAGAAAATACCTTTTTTAATTTCGTTAGAAAATTTTTTTTCATATTTTTGTCTTCGAGAGTTTATCTCAATTAAAAAATTTAATATAATCCTATTTATGTTATATTTCATTACCCATGTCTGTGATTTCATTATATTATCAAAAGACATTATTATATTAAAAATAAATCGTTGACTATGTACAAAGTAACTATATGGTTCGCTCAAAAAAATGGATTTATTAAACGTAGAAACTATTACATTATTCTTTACAACATCTGCAGCATCTAAATTAAAGTTTGGTACATTCATACCACAGATATTACAAAACGCAAGACCGTTTTCGTAATAAATGTACTCAGTAGCAAAATTATTTACTTTTGTAAAATATTCCGTATCAACTTTTAGTGCTTCTATTAGTTTAACTTGATGTTCACATGGTACAAACTCTATTTTTACTTCACCTCTTAAGGATTTTGGTTCGATTACTTCTATTTTTTTAATATTTTTTAAATCCTTAAACGTAATTTCTTTTTCTTCTATTAATTTTGGAAATAAGTCTCGAAGGTAAAAATTAGATAAAAATATATAACTATACAAATGTTTATTTTTTATTTTTGGTATTATAATGTTCCCCCAATCTATTAAACTGTTATTATCCTCAGAAAAGGAATTTTTATACGTTTTATTAGTTTCTTCCAAATAAACGTACTTAACATCTAAAAAGTCCTTTATAACAATAGGTATTGAAACTCCATCCACTTTATATAAGTATATATATCGCATATTAAGTTTTTTTTTTGAAATAGGTATTCCTATATGCCTGCATAAGTATGCAAAATCTAAATATTTTTTAGAAAATCTGAACTGGTCAATGTTTTTAGATACATAACTTATCATATCTTTCATGTTAAATGGTATTTCTCTAACTTCTGGATCAATATCTTTGTTAAAATTAGATGTTAAATCACTAGTTTTATAATTTATCATTATGTCGCCATTAAGATCATCGTTAACTAATACATTTATCCTTCTTTGTCTAAGATATACATCTAGGTTATCAAAAAATAAATTATATAAATTAAACGTCATCTCATCAGTTGTAGTATTATACTCATTTATAAATAAACTAGATGTTATCTCTTCCTTAACGTTTTGTTCAAACTTGTATCCTATATATGAAAATATGGATATTAATGTTTGATCGTCAACTTCTAAATTTTGTTCTATTGTCATATATAACAACCTAATATCTTCTTCAGTGATTGTAGAAACATTATATAAATTAATAATAAAAATATTTTTATTATTTGATATAAAATCCGAATATGATTTTGGATTTATATTTGGATTAAGAATGTATTCTTTAATTTTTGGTATAATCTCAATTAAAACAGATTCTTTAGTTTCCATTTAAATATTATATACAAAAGGCAAAATTTATAATAGATTTATTTAATAGTGTACAAATTTTTTATAAAAATGAAATAATAAAGACTTGTTTTAAAAATAATTATAAAAATAGCTTCATATAAATACAAACAATTATTAAAAAAATGTCGTGGTCTATAAATTTGGGAAATAACGGAGATAATTTTAAAACATTAGAAGAAATACGAGCACATGTAAGGTCGACAACTGAAAATATGGATAAGGTAGCGGACGAAATCTTTCCGAGTGATGTAAAAATTCCAACGACTTCCTCTTCTTCAAAGCAAAAGAAACCAGTTGTTCGTAAGAAAGCGGTTACGACAACAAAGAAAAGTAAAAAGATTAAAGAAAAAACCCCTATCGAAGATCACGATGACGAAAACGATAATGATAATGATGATGAAAACAATGATAATAATGATAATGATGATATTAATAATATTGAAGCAGATGATAAAAATAATGATACAAATAATAGTGACACTGAAGAAAATGAAAATGACGATAAAAAAGAAGACAATAAAAAGAGCAAAAAAAGCACCAACGACAATAATCAACACGACGATGACGTGATCGATAATTCTGATTTAAAAATTGCAACTGAGTCAATAATTAAAGGTTTGAAAATACTTAACAATAAGGTATCGTCCGTTTCAACTGTATTGGAAGATGTACAAGCTGCGTCAATAAATAGGCAATATAGTTCTTTAATTAAGAACATAGATTTGCTTAAATCGCTTGCGGAATGTGGCAAATCTCAAGTTATTAGGAAAAAAGTTAAAAACACTAAAAAGTAAACAATCAAATATTATGATTTTTATTTTATTAGCGAATAAAAATGAGAGGATTATTTTATAACGATGGAAAACTTTTTACGGATAAAAATTTAACAACTCTTGTATCATTTGATAATCCTACTTATGAAATTATAAAAAGGATTAGGATACCGCCCCACTTAACTGATGTTATTGTTTATGAACAAACGTATGAACAGTCATTAACTAGATTGATTTTTGTTGGTTCAGATTCAAAGGGTAGAAGGCAGTATTTTTATGGAAAACTGCACGTAAAAAATAGAAACTCCAATAGAAACAAAATATTCATAAAGGTGTTTAATGTTATTAATAAAATAAATGAGTTTATAGACAATAATATTATTAATGAAAAAGATAAAAATGATGTTTTGTTCCAATCAGGAGTGTTTATGCTAATGGAAACAAGTTTTTTTATCAGGATGGGAAAGATGTGCTATCTAAAAGAAAATGAAACGGTAGGATTATTAACTTTAAAAAATAAACATATAAGCATAAGTAATGGAAAAATAGTTATAAAATTTATAGGAAAAGATAAGGTTCCGCATACCTTTATAGTACATAAATCTAATAAATTATATAAACCTCTTTTAAAATTATTTGATAAAGAATTTCCTGATAACTTTTTATTTTCTAAGTTGAGCGAACAAAAAATTTATAAACTAATGAAAAAATTTAAAATACGATTAAAAGATTTACGTACATATGGTGTAAATTACACGTTTTTATATAATTTTTGGTCTAATGTAAAATCGTTAAATCCTATACCATCTACAAAAAAGTTGATATCTACATCTATAAAACAAACGGCTGAAATTGTTGGTCATAGTCCTTCAATTTCTAAAAGTGCATATATCGCAACAACTGTTATAGATTTTTTAATTCAAGATTCTAATATATTAGAAACAATAAAAGAAAAATCGTTTGAAGAGTTTATAGAATTAATTGTAGATTATATAGTAAATAGAGACACTATATAAAAAAAACCTTAAACGATAAATGGATGAAAGATTAAAGACGATTTCTATGACCTTTTTTTATGGTGAATTAACAACTACAGATATAATGGTTTTAAAGGCACACTTGTTAAACATTTTTCCAAATGATACTATTTTTTCTATAAATGAGGATAATAAATTTATAATTGACTTTAAATATAACGACTGTTTAGCTTCAGGTTATATTAACCAAAAAATAATACCTATAACTCCAGAAAAATACAATGAATATTCATCGATGATAGCTAAAGAGTTGACAAATTATAATATTATCATTGATGATATTAAGGGGCATATACTATCATCTAAAAAACTTAAAAGATTTATTAAATTTTATAAAACAAATAATAATAAACAAAATAAAAAAATAATTAACGCAAGTAAAAAATTAAATATTGCGTTAAATAAAGGAATTGATTATGATTATATTAGACATATTAATTAAGTGAAAAAAAATAAAACAAAAAATAAAATAATGGACGAAAGAGACTTAGAAAATTCATTAAGTGATTATATTAACAAAATAGTTGAAATATACGATGAAATTCCAAATCCTACATTATCTAATGATGTAAATCATGAAGTAGAATTAGTATTTATACAACCCCCAATAATTACATTAAGTAATGTATATAATATTTCATCGGTTACAGAATCATATATACTTTTTACTGTAACCAATAAAGGAGAATGTATAAAAATTAGAACTAAAGTGCCCATGTCAAAAATACATGGGTTAGATTTAAAAAATTTACAATTAGTTGATTTAATCGATGATATAATTTGGGAAAAGAAAACACTAATAAAAGAAAAAAAAATTGATAAACATTGCATTATAAGATACTCTTCTGAAGAAAGACATGTATTTTTAGATTACAAAAGATATACTTCATCTATAAAACTAGAATTAGTTAATTTAGTACAAACTAAAGTAAAAAATATAGTGGTTGATTTCAAAATTAAATATTTTTTAGGGTCTGGTGCACAAGCAAAAAGCTCATTGTTGCATGTCCTTAACCATCCTAAATCTAAACCTACTCCTACTTTAGAATTTGAAATCATTACACGTGAAAAAAAAAATTCTCGTGATATATTACTAGATGAATTACATATACTTTTTAAAAATATATTTATGGCAGATCCAAAAAATGTTTTCCTCCCTACCGTTATTTTTACTAGTCCTGTAAAAACTATAATGTTAAAAAAACAAGAAATACATAATATAGAATTGGATAAGTTATATATTACAACAAAGACTGATGGGGTTGGTACTTTAATAAAAATTAATAAAAATGGTATATTTTGTTATTTTACTCACCTAAACTACATAATTAAATATAACCTGTTACGTAAGTTTGACGATACTGTAATATTATACGGAGAGGCGATAAAACAAAATCAAAAATGGTTCATATACTTAATAAAGTTATTGAAACCAGAATTATCTAATAGATTTAAAGAACGAGATTTTTTGATAGAAAAGTTGACAAGTGATATATCGGATAGAATTGTGTTCAAAACAAAAAAATATGAAGGACCGTATGAAACACATTCTGAAGTTATAGATTTATTATCTACATATCTTCCATTACAAACCGAAGGCGTGATTTTGTTTTATTCAGAAGGGAATAAATCAAAAATAGATTATAAAATTAAACATGATAATACTACCGATCATATGATAAATGCCGTATATAGATATATGTCAAGTGAACCCGTTATTTTTGGAGATAAATATACGTTTATAGAATTTAAAAAGTTTAGCGATGAACGGGGATTTCCTAAAGAGCATGGAACGAGTAAAATAATACTAGGGGATAACATAAAATATTTAAACAATATATATTGTATCGAATTTAAAAATATTTATAACGATGTAGGATTAAAAAATATAGTTTTACCGATAAAATTTATTTCAGAATTTTCATTTAATGGAGATTTTTTAAAACCTAGACTGGATAAAACTATGAAATATTTCTATAAGGAGTATTATGGAAATCAATACCAAGTAGTATTAGAACATATTAGAGATCAAGAGTTAAGAATTAATGATATTTTTGACGAAAATAAATTATCTGATTTAGGAAAGAAGTACGTAGACGACGAATTTCGACTGAATCCAGAAGTATCTTATTTTACTAATAAACGAACTAGAGGCCCGTTGGGAATTTTATCAAATTATATAAAAACTCTTTTAATATCTTTATATTGCTCTAAGACCTTTTTGGATAATCCAAATAAAAGAAAAGTATTAGCCGTTGATTTTGGAAATGGCGCAGATTTAGAAAAGTACTTTTATGGTGAAATAGCATTGTTAGTAGCAACTGACCCAGATAAAGACGCAATAAGTAGATGTATAGAAAGATATAACAAATTAAATTCGGGAATAAAATCAAAATACTATAAACTGGACTACATACGAGAAACAATACGTTCAAGCGTCTATGTTTCTAAAATACGCGAAGTGTTCTTTTTTGGAAAGTTTGATATTGTAGATTGGAACTTTGCAATTCATTATTCATTTCATCCAAAACATTATTCTACAATAATGAGAAATTTGTCAGAATTAACAGCACCTGGAGGTAACGTATTAATAACCACCATGGATGGGGATAAACTGTCTTATTTATCAGAAAGGAAAGTATTCATTATCCATAAAAACTTACCGAGTAGTGAAAATTATATGTCTGTTGAAAAAATAAGTGATGATAAAATATTAGTTTATAATCCATCTTCGATGTCTAAACCAATGGAAGAATATATTGTAAAAAAATCAGATATAAAAAGAGTATTCTCAGAATATGGTTTTGTGCTAATAGATTGTGTTAATTTTGATACAGTTATTAATAGAAGTAAAAAATTCATCAATTATGTTTCTAAAATGGAAGAACGTCAATCAACAAAAAACTTTTTTGAACTTAATCGGGAAGCGTTAAAGTATGACAATATAGATATAGAAGAACTTCTAAATTATTATGTTGTTTATGTATTTTCCAAAAGGTAAATAGTATGGAATAGTTGATATATTAGTTAGTTTATTAAACTTAAACGAATTAAATAAGTGTAACAATATTAAAAGAATACCTTCGGAACCGTTTGGTATCTCGGTTAATGGTATCAACGCATTCTTACGTTCAATAATATTTACACTATATGTAGGAAGTAGTGCATTTGTGCTTTCTATTTTTTTTTCGTCGATAAAAATTTTTTTTAAAACATCTATATCATTTCTATATAAAAATTTTGCAATTTTAAAAAATGTACCATTGTCAAATCTAGCCATAGAATCATATATATGAATAGCATATAAGGTTTCATCTAATCCTTTTTCCAATAACAAAAATTTTTCGTTATAAATTTTTTCTATATCTATAGGGCATATAATATTCCTTTCTTTAATAATATTTGTTAATCTTTTAATTTCAGATTCCATAATGAATATTTGTATAATAAAAAATAACGAATATCCAAAAACTGATTTGCATGACGTAACATTTTTTCTATTGGGAAACCATAAAGGTTTTTTTGAAAATGTTTTAGAAAAAATTAATAAATATAAATATTTTTTTTCTCAATATGAAGTAAGTTCGGACAACGATGGTGTGTTAGAATTAAAAAATGTAAAGTCTTCTCACATTATTAAAGATAGATATGTTACAACAAGAGAATTTATTTTGTCAGGATGCCAAAAAGAATGGTGTAATGAAATTATTCAAAAAAATGATAATATCACTAATTACGATACAATAATAATACAAGATATTACTTATTTTGAAAAAAAAATATGGAAGAGGTTATTATTTGTCAACTGCCCAGAAATGAAAAATAGTTCATATGAAATCTTTTTAATAAATCCATTTGTAATAAAAAACAACACAGATATATTTAAAAATTTGATTTTAAGGTCATCGATTAACTATTTCATATTTGATAATTATGCGCCACTGAAAAAAATTTTAACGCATATATACCCATCCTTTAATATAGAAAAAACGTATATATATATAAACTTTAACGAAATAAATAATATTAAAATAATAAAAAAATGTTATCATAGAAATAAAATTAGAGCTTTCGTTTATGCGTGGTTTAATTTTCAAATATGTGAAAATAGTGAAATAGAAAATGAAAAGGTAAAAAAAAAATACAATGATTTGTATAGCTTAATATGAAAACCATAAAAACAAAATCTTTTCCTTATTGTATAGAATATCATGACGATTGGGAAGCCGTTATTAATCAATTGGTAGATTTATACGATGAGATAGCTGAATGGATTTTAAAAGATGAAACATCTCCAATTCCGGAAAACTTCTTTAAACAATTACGGAATCCACTTAATGATAAACTAGTGTGTATATGTGGAATAGATCCGTACCCAAGGGATGCGACAGGTGTTCCGTTTGAATCACCAAATTTTTCAAAAAAAACAATTAAATCAATAGCTGAAACTATTTCAAATATAACAGGTATTAGTAACTATAAAGGATACAATTTGAATAATGTAAAAGGTGTATTTCCGTGGAATTATTATCTAAGTTGTAAAATAGGAGAAACTAAAAGTCATTCATTACACTGGAAAAAAATTTCCAAACTTCTTCTTCAACATATTACTAAGTATGTAAATATATTGTACTGTTTAGGAAAAACTGATTTTTCAAATATAAAATCTATAATAGAAGTTCCGGTCACTACAATAATAGGCTATCATCCCGCTGCTAGAGATAGACAATTTAGTAAAGATAAGTCTTTTGAAGCTATAAATATATTATTAGAAATTAATGGTAAAGAAATAATACATTGGGAAGAAGGATTTTGTTATTAGGTTTAAGCTTTAGTGAAATTTTAACTAACTTGTGTATTAAAAATGGCAAGTCATTTAGTCAATAATGAGTATATATTTGTTTTAAAAAAAATTGGCGTTCATACATCTCATAGAGAGAGTGAAGATCCGAGGTTTGTTGATATTTTTACATGTGAAGAATTAGAAAACTATATATTAAAAAATCCATCATGTACATTATTCGAAACATTAAGAGATGAAGAAGCATACTCCGTAGTAAGAGTATTTTTTGACGTTGATTTGGATATTGTATTAGATGAAATAGACTATACTGCGGCTTTAGAAGATTTCATATTAAATGTTACAAAATTTGTATCTATATTTTCTTCTCGGGAATGTGGTACGAATCAAAATATTGTGTTAAAATGTATGAGATCAAATTTTTCGCTTACAAAATCTACAGATACTAGTCGTACTAGTTTTCATATAGTATTTCCCGATACTTACACGAATATGGATACTTTAATTTATATGAAAAAACCATTATTAGAATTTTTAAGATCGTCAGAAAATCCATTAATAAGATCAATTGATCCTGCAGTGTATAGGAGAAAAGCAACACTAAGAATAGTTGGGACGCGAAAAAATCCAAACAATGATAAAATTCATATTAGACAACCGCCTCACAACAATATATCTGATTATTTGTTTACTTATGTGAACATGAATGAAAAAAGTTGTTATTTTACATTATCAAAAAAAGTAGACGATAAAAAGGCAGATGTATTATGGGAACCAAATTATATACACTTTAATGATGCTATGAAAAAAGTTTCTAAAATAATAATAAACGAAATTGTTAATATAAACGATTTTAATGTGAATAACTTTACTAGTATTCCAATAATAATAGATTATATTATGCCGTGTGCGTTATGTAAAAAAAAAATACATAAACATCATCATCAACTTACATTAGGAAATGGAATTTTAAAAATATTTAAAGCTGGAAATCCTCACAGTTGTAAAGTTAAAGCGATAGCATTAGAAGGAAATAAGCTATTTACTATATCTCAGCTTATAATAGATTCAAATGTTATACATTTAACAGAACGCGGAGATCATGTAGTATGGATAAAAAACTCTTGGAAATTTAATACAGATGAACCCCTTATAACTAAATTAATATTAAATATGAAAGATCAACTACCTTCGGAATATATACCAGATATATTATGTCCTAGAAAACGTAAAGTAATTGAAAACAATTTAAAAGATATGTTGGTTGATACAATCGAGACAGATACATACCCTTTTATGCTTCCATTTAAAAACGGGGTATTGGATATATGCAGTGGTAAATTTTACAAAGGGGCAGAATCAAAAAAATTTATTTGTACTGTATCAACTGGATTTAATTTAGACATTGAACAGTATTTAAATGAGGATTCGCAAGAAATGAAAGATCTAAATAATATTATAAACGATATACAACCTTTAACCGAAGAAAATAAAGAAAATAGAGAACTATATGAAAGAACACTTTCTAGCTGTTTATGTGGATCAACAAAACAATGTTTGACATTCTTTTTTGGAGAAACAGCAACAGGAAAATCCACAACGAAAAAATTACTTCAATCATCGATAGGTGAATTATTTATAGAAACGGGTCAAACAATTCTTACTGATATTATAGATAAAGGCCCCAATCCTTTTATTTCTAATATGCATTTAAAAAGATCAGTATTTTGTAGCGAACTTCCTGATTTTTCATGTAGTGGAAGTAAAAAGATTAAAGCGGATAACATAAAAAAACTTACAGAACCTTGTATAGTTGGGCGACCATGTTTTTCAAATAGGATACATAATAAAAATCATGCATCTATTATTATAGATACAAATTATAAACCTGTATTTGATAAAGTCGACAATGCATTAATGAGACGAATAGCATTAGTAAAATTTAGAACACATTTTTCGCAATATTCTAATTCTGACTCTGTAAAAAATAATGCAGCATATGATGATGTAAAACCATTAGACGAAAACTTAGATATGAAAATTCAAAAAAATTACTTTAGGTATGCATTTTTAAACTTACTAGTTAAATGGTATCAAAAATATCACATTCCTACAATGAGATTATTTCCTACACCTGAAGCAATTCCAGATTTTGTATTTCAACTTAGAGTTGGTTCGTTACTCATCCCAAGTTCTAGCACACATTTAAAGTTTATGTCTTCGTTATCTAAAATAGGATATGTATTAGAAAATGACTTAATGGTATTACCCCTTCAGTTATTTCAACAAAAAATATCAAAACATTTTAATATAAAAGTATACGGTAACGACATTGAAAGTTTTATAACAAAACATAAAAAGTTTGCTAATGTTAGCGAAGAATACCTAGAGTATATATTTATAGAAGACATCATATCTAAATGAACTTAGGAATAATAAAATTGTTTAATAATCACGTAAATAGTATACCAAATATATTACCACATCAATTAGCTACATTAGACTATTTAGTTAGAAGTATTATAGATGAAAATAAAAGTGTGTTACTTTTTCATATAATGGGATCTGGAAAAACAATTATTGCATTATTATTTGCGTTAGTGGCGTCTAAATTTAAAAAAGTTTATATATTAGTACCAAATATAAATATACTAAAGATTTTTAATTATAGTATGGACGTAGCGATAAATTTGTTTAATTCAGATTTTATTCTTGAAAATATTTTTATCTATTCCACCACAAGTTTTTATTCTCTCAACTATAACGATAATGTAATTAACTACAACGGATTATCTAGATATAATAATGCAATATTTATAATTGATGAAGCCCATAACATATTTGGTAACAATACGGGAGAACTAATGACAGTAATAAAAAATAAAAACAAGATTCCTTTTTTGCTACTTTCTGGCTCACCAATAACTAATACACCGATAACACTTTCAAACATAATTAGCTTAATGTCAGATGAAGAAATAAATTTTGGAGATATTATAGTACAGGGGAAAAAAGTATTTCAAATTTTGTTAAATGAGCACGGCGTCAATGTTTTAAAAAACATATTAAGAGGGAGAATATCATACTATGAAATGCCCGATTCTGATTTACCAATGATAAAATATCATGGTAAAAATTTTTTAGATACACGTGTAGTATATTGTAATATGTCTAAGTTACAAGAAAAAGATTATATCAACGTAAGAAAATTATGCAATAATGAAATGTTTGAAAAAAATATGAACAATGTTTCGTTAGCGGTATTAGGGCAATTAAATTTGATAAATAACTTAGATATTTTGTTTCAAGAACAAGATAAAGAACTTTATCCAAATCTTAAAATAAGTAATGGTATTTTATATGGAGATGAACTCATTAACTTAAATATTAGTTCTAAATTTAAGTATTTCATTGACAAAATAACTTCATTAAAGGGGAAACATTTTATATACTTTTCTAACTCAACATATGGTGGATTAGTAATAAAGTATATAATGCTTAGTAATGGATACTCAGAATATAATGGGTCACAAGGGACAAATCCAAAACTTATAAATGGAAAACCTAAAATGTTTACAATAGTAACTAGTAAAATGAAGTCATCATTAGAAGATTTATTATCTGTATACAACTCACCAACAAATGATGATGGGAGCCAGATAATGTTTTTATTTTCATCAAATATTATGTCAGAGTCATATACTCTAAAAGAGGTTAGAAACATTTGGTTTATGACAATCCCGGATACCTTTTCTCAGTATAATCAAATCTTGGGAAGATCAATAAGAAAGTTTTCATATAATAATATAACAGAACCAGTTAATGTATATTTACTAGCTACAGTGTATTCTGATTTTGATGACGATATAACATCGTTAGATGATTACAGCTTGGATGAAATAAACACACTTCCATTTGATATAAAAAAATTATTATATTTAAAATTCAAAACTAAAGAAACAAATAGAATATATTCGATATTGGAAAGTATTTCTGAAAATTATACAAAGGAACCACATCCGTATATTATCGAAATTGTATTAGGAGAAATTATAAGACAATTTTTTTATCACCATTCCAGAATTAAGTTTGATAATAAACAACTAATAAAAGAAATATCGTCTGTGTTGCAAAATATAGAATTGGTAAAAAAATACATTGATGATATTGTCAATGGACACTTTTTTGTATCGAATAAAGTTTTTGATAAATCGTTATTATATAGATACAATGATGAAATTATAACTGTTCCATTTAAATTATCGTATGAACCGTTTGTGTGGGGAGTTAATTTTAGGAAAGAACATAATCTTGTATCATCGCCTTAATTTAAATATTTATTAAAGATAAAAATGTCAACTTTTATAAAAAATGTTTATTTGCCTATTAACTTAAATCCTCATGAGTTAACATTAGACATAAAAAAAAATATAAAAGATGCAATTTATAAAGAATACTTACATCGAGAATCGGGTGGGATAATGGCTAAAAAAATTGATATATGTTTTGATAAAGAATTACCACTAGGTGAAATTATAAACAATCACATAATGGTAAAAGTTCCGTGTATTGTTACGTATAAATATTATAAAAATGGAGACATTGTTAGTGGCACATTAAACATAGAAGATGAATCAAATATAACAGTTTTATGCGGAGATTTAGTTTGTAAACTTAATAGGGATTCTGGATCTGTCTCTTTTAATGATTCCAAATACTGTTTTGTAAGAAATGGAATAGCTTATGATAACGGATCAATGGTCACAGTTATATTAAAGGAAGAACAACAAGGAATGAACTCCAACTTTGTTTTTTTAGCATCTATAATGGATCCATCTAATTAAAAATGAAAAATAATAACGTTTATACCCGAGTTATTTAGAGATGTTGACAACAATGATCGAAACAGATAGAGAAAACGTTACAATTGAAAGAGTAAACGAAATACCAGATGAAAAAAATACACACGTTTTCGCTATTTGTATAACCAGTGATGGTAAACCATTAATTGCTGCAAGGAGGAACTCGTTCGCATTTCAAGAAATAATGTCACAAAGAGTGTCACCTACGTCGATATTGAAAGTATCTAAACACTTATTAAAATACATGTATAATAACGAAATAAAAGAAATTCAAAGAAGACTTTTAAAAGGTTCATATTTAAACGTATCTCAAATGACTAACAACTCGTTTGAGGAATTAATATTATTGGGTGGAAAAATAAATAAATCTGAATCTATAAGTGAGTGTCTACAAAGAGAAATACAAGAAGAAAGTGATTCTAATTTAACAATTAAGTGTTTTGGAAATAAATGTGTGAAGTTAACAATATTTGATAAGTTGTTTGATAAAACTTATATAAGTTATTGTACAACATGTTATATAACAGAAACAATATCGCAAGCTATATCTTCCATTATATACAACGTCGAAATTAGAGAGCTAAAGTCGTTGTTAGAATGTTCTAAAAATGATAAGTATCATTACTTATTGTTTATTTATAATACGTTGGTTAATAGTAAATAATGGATAATTATTATCCTTCTAGTTTATTTAATGATATAATCAAACGGAATAGAAAGTTGTCAAAAACATATATATTATCTAGCAATAATCAAAAAATATATGTTACTGGTTTTAATAATCAAAGCTTGGATAACGTAAAAAAAGTTTCTATTTCTATAGTAATGTTAACAAGTGATAACAAATTTTTAGCATGTTCTAGAAGACATAGTTTTTTATTTACAGAAATCATAAGATCAAAAAACATATTTCGTAAACGGAGATTATTTTTAAAATATTCAAACTTTTTAAAAAAAAACGAACGTATTATTTTATCATCCGAATTAGATATTAAACTGTCTTCGTCAGACCATGAAAATGATTTCAGTAATATAATATTTCCAGGTGGGGTAATTAGAAACGAGGAAGACGTTATTAAATGTCTTTCAAGAGAAATTAAAGAAGAAGTTAATATAGATAGTAAAGATATTTTTTTAGATTATAGATTTTTTATACACATGTTAATAGAAGATCTTCTAACAGATAGGTTTTATGAAAACATATTATTTTTTGGAAAAACGTTTCTAACGAGTAATGAGATAATACATAATTTTTTTTCTAATAAAGAAATAAAATCATTAGTTTTTTTCGATTCTTTATATAATGGAATAGAAGGAGATATAATAAGATTTGTGTTAGATATTTCAAGATTAAAATGTTTTGGAAATAAGGGTTATGAATTATATAATAAAAACACTTTTAAAAGTTTAAAAAGCTTTTTTTAAAATCATTAGTCGTCTGGCAATATATAAATCAGTTTATTATTTTGTATTTTTATAATTGGATTTTCTGGCATTGTTTGTAAAAAGTTTCCTTCAGCGTCAAAAATTTTTCCATCGTTGGTTTTAAATCCCTTATGTATATTTACCAATCTTTTTGATGTAGAAAACCATATATTTTCACCTTCTACAACTTTAACTATTTCTTTTGTTTTAGCATTCATATCAATTGATCTTGATATTAGTGGTATCCACCCAGATTCATCATCTACAGGTGAAAAGTTTTTTTGGTGATCGTATATCCATTCAATAGATGATTCCTTTAACACCTTAAATAATTGAGAAAATTCTTTTGATTTTGTTCGTATAATTTCTAATAGATCATCATCAACTGTAGATTCGCCATTTGATAATCTAGCGATTATAAAATGCACATTAACATATCTTCTATCTTTAGGAGTATTTACATGACTGTTTAACCTAATAGCTCTTCCAATTATCTGTCTTAGAGATGCTTCATTCCATGTCATATCTAGTATAAAAATATCATTTATAGAAAAAAAACTAATTCCTTCACCTCCACTAAGTGAAAACACACAAACTTTAATTACTGTACCGTCAGTATTTACTTCTTCGTTGAATTTGGATACCATCTGCACACGAGTATTTTTTGTACGTGAAGAAAATTCTATATAAGATATTTTAAATACAGAAAAATATAGCAATAAAATTATAATTCCTGATTGGTTCACAAATGGTTCAAAAACCAAACACTTTCCAGGAGATGAAAGTATGGATAAACACACTTCTGTAAATTTACAACTTTTTTCTTTTAGTTCATTATACAAAGATAAATCTGATGCATTAACGTCGCCTTTTAATTCTTCCCCTTTTTTGAAGTAATCCAGTGCTTTATCGGAAAAATATCTACCTTTGATAGAATTTTCAAAATCTTTATATAGTGTATTAACTTCTTTATTAAAATCATCTAAGTTTCTATCTTTTTTTTCTGGCAACACGTCAAACGAAAATGTTGCAGCCATTCTTCTATAAATTCTAAACGATGATATACCAGATTTAATTTCTAACATTTTAGCTTTTTGGTAAATAAATTCTTGTTTTGGTGTCATGTTTATATATTTCATATACACTTTTTTTCTAGCAAAAGAATCTGAACCATCTACATCTTCAAAAATAGAAAATTCATTATTTACAATATATGAACAAATTCCTCCTAATTTGTTTATTAGTTCTCTTTCGTTAACCAATTTTTTATTTTCAAATAATGATTGTGTTTGTAATATTTTTGGTCTTAGTAAGTTTACTAACATGGTAAACTCTCTAACGTTATTAACAATTGGTGTCGCAGATAAACATATAAGCTTATGGTTGTGATACGCTATATTTTTTACTAAGTAATTATATACAGATTTTGTAGGACGTATTTTTCCATCCTCTTTAATTAATGATTTAGAAATAAAATTGTGACATTCATCGATTATTACACATATTCTGCTTCTAACATTTATAGTTTTTATGTTTGTAAAGAACTTATTGTGAAAATTTTTATCATCATATGTTATAAAAATACAGTCTTTGATTATCTCGGGAGAATATTTCATTAATGTATTCATCCATGGATCATCGACTAGTGCTTTTTTAACTAATAAAATTATGGTCCAATTTGTATAAAAATCTTTTAAATGTTTTAAAATAAAGACAGTTGTTATGGTTTTACCAACACCTGTTTCGTGAAATAGTAATATCGAATGCATTTTATCTAATCCTAAAAAAATTCTCGCAACAAAATGTTGGTACGATTTTAGTTTTATTTTGTTTACTGGACCAACCATTTCAATTGGCATTTTTTCCGTAGTGCGTAACGCATAGTCTATGTATGCGGCGTGATAACTACTCATATTTATTTAATATTTTGTTATAGTAATCATAATAAAATTTTAACTAATTTTTTTTGATATTCATTTTCAATACTTTTATTTTCCGAAGTCATACTTGAATAAAGTAATGAATGTACATAATATAGTGATTCGTAAACAGATGTTGAAAACTCTAAAAACTTGTTTATGAATTCTTTATCTACTGGATACTCTAATATGTTAAATTGGACATTGTTGGCATCCTTCCAAATAGAAGATTTTGTAGTTTTTTTTATTTCAGCCATAAAACTATCAAAATTTTTTTTATATGGTTCGCTCGCTACCATAATCGCACTATCTTTTAACCAACTATCACTCGCACATTTAAACAATCGTACCGACTTGAATAAGCTACAATATACCCTTAACGTATCGACAATACACCTACCAAACAACGTTGGTAATTTTATTATACAAAACTTTTCTACAGACATTTCAAATAGCGGTCTAAATACTTCAAATGTCAACATTTTATAGTCACTCACATAAAGATTATTCTCGTTTAGGTAGTTATTAGATTTTATTAAATCTCTTTCTTTTTTAAAAAATTTATTTTTAGCACTTAAATCTGCAACGACAGCGTCGGCTTTTATATTTTTTCCATAAGATTTTATAGATTTTATATTTAAAATTTCATATACTTTTTCTAAATCGGTTTCTGGTATCATTAAATCATGTGTAAATAAACTTAATAAATCCGTTGGTAATCTATTCAAATCATTTACTCTTGATAATTGTAAAAAATAATTAGTTCCATATTCCAAACTAGGTAGAGGACTTTTCCCTAAAGTTAAGTTTAAATCTGGCAAATGTTCGTAAAAAGGTAACAGAATGTAAATTCCTTCTTTAATATTTTTTATAACGTCATCCATTTATTTATTAGGTATTTTGATTTCATTATTATACTATTTTTATTCTTCCGTTATTTTGATAACATTTATACCATTTTCATTAGTTACTTTGTATAGGTCATTACAAACTAACACAATTTGTTTTGATACATAATAAATTGGATTATTTGGATCAAATGATGAAAAAATAACTCTAACTGAGAGTTTATCCTTTCCTCTGGCTGTATTAGAAACAATAGTAGTTGGTTTAAAGAAAGTATGTGGTGTAAAATTAAACTTCAAAGTCCTTGTACCCAATACACAGTTTGATAGTAGATCGTTATAAATCTTTGAAATAGGATTAGTTTCAGAATAAAGTACATCATTTCCAAATCTAACTTCCATCCTAGAAATAATATCAGTTTTATTTTTAAAATCTATTCCTCTTATAAAGGGATCGTTAATAAATAAATCTTTATTTTTTGACGATTCTGATCTATTATCTCCATTATAAACGTTTCGTTGACACGACCATAAATTAACCGGAATAGAAACATCTGTAATACTAATAGAGTGTGATATATGAGAAAACATAATTCTTTTTGTGGATTCGCTATAACTCCCAGTTATTGTAGAAAACTTTTTTGAAATATTATACGTGATAGAGTTTTTTCGTGTTCCAAAAACTAAGATATTTGAATGATAATAGATACTCATTCCACAAGGCACGTTATCAATTTTAATAAAAACATCAACATCTTGAATATTTACAATACCGTTTGGAGGCACCTCGATAAACTCCGCACTTTCTGGAAAATAGTTAGGTGGTTCCTTGCTTACAACCACTAAGTCTTCCAATAATCTTTCAACAAAAACACAAATGAAGTCTCGTTCACTTTGTGAGTAGCCCGGGTAAGCTATAAATTTATTGTCAGTATTTCCGTAATACGGTTTTACATAAACCGAAAAAGATGTAACTGAGTTTATTTCTGAGATTACGGCAGTTGATTGGTTTATTTGTCCTAACACTCTACGAGGTATTTCTATATATGATTCTTTTGTTTGAATATTTTTAACCATGTACCCGATAAAGCTGAGTTCGGTTACGTAAATAAAATCTTTAACAAAAGATTCATAATCAAATGTAGAATCCCTAATTATTATATCAGATACAGGATTAAATGTAATAGTAACAATAACTTTTGAATCTGATAATTTTAAACTACTAAATGTTTTTTCCATATCGAATGGGGTTTTTATATATACATATATCGTAGCAGACTCCTTAATCGTATCGTTATTTGTTAAACCAGTAGAGATGCTATTAAGTTCATGACAAAATCCAGAATTGTTGAGAGCCGTTTCGTTATTTTTACAGGAATAAAACAAGTCTTCTCCTGACGATTCCCAAATTGTACCATTATTGCATGAGATAGAAACATGTTGTATCGACTTATAACCAACATAAGTAACATAACCAAATTTGCCAATTCCTTTTACTTCCGGAAGATCTATACTTAACATTAAATGACTCAACGCTGTGATGTACTGATCTCTAATCTCAAACGTCGAAACAACATTTTGTGTACATGACCCTGTCGATGAAACGCCGTTGAGGGTTATATACTGTGGCATATATAATGTTGGATTTTGTATATCCACACCAAAAACATTGTGACGTTTAATATTATCATCGTTTCCAATTAATGAATTAATTACCGTGTTGTTCATTGTTTATTTATTTAATGTATAAAAGCTTAAAGTAAACTACGTAGAGAAACATGTATGGATTTATCTTTTTCTTTATAAAAACAACTCCCATAAACACCCTCTTTATTACGTAATGTGTTAATTATATTAATTACTTCTTTGGGATTATCATAAAAAACTAGAGGCAATAATGTTACCTTAGGTTCCTCCCTCAACGCTATATGAGATTTCACCATTGATGCAAAAGAATCTCTGCATATAGTGGAACAAAAAGCCCCAACATTATTTCCTTTTATGGTTTCTATACATAATGGTTTTATAATTAAAATTTGGGAACAAAACCAACAATAACTATTATCTGAATTTGTATCTATTGATATATCCGCTACGGGTACGTAATATTTTGGAAGTATCGACGAAAAACATTCATTTTTTGTTGATTTGGCAACTGATTTAGGAGAAGAAATAGTAATATCTGGCAACGTAACTCTTTTTGCCATTTAACTAACACGTTTATTTTTAACTAAAATTATATATCTAAAGAACAAGTTGTAAATTTTTCATCAGTAAAAACATACTCGTGACCAAAGTCTATTATATTCGCAGTCTTTTTTTTATTTTTCATATATATTAAGAAATTTTCCCATATTAATTGATTACTGTTATTTTTTGTATAATTTTTAACTGTTTGCGGTTTTAAATTTTTTGTAACAAGTGTTATGTCAAAAATTTTATCTAAAAAAAAGGAATAATTTATCGTCTTAGATGGTGTATTTTCTTGGCAAAAAAATACTAAATGTTTAAATATCTCAACAACTTCATTAATTTTTTCCGTTGTTAAATTTAACTTTTCAGATTTTACTTGATTTATTATTTCAAAAACAAGTTTATAGTCCTTTTTATTAATTCGTTCGTGTGCTTTTAAAAAACTAGACACAAAATTAGCATCAACATCTTCAGAAGAAATTTGATTTTTTTTCATTAAATCTAATAACTCTGAAATAATTTCCCCCGAACATTGATTAGATAAAAGTCGTCTAAGAACATTTCTTAAATGTATAAGTTTATTAGAAACATGAAAATTAGATTTTTTTTGAACACTCTTCGAATATTTTTGAAAAACGGATTCGCAAAAAATACAAAACTCATATCCTTGTTCTGAAACTATTCCATTATGAAAGCATCCACTGCACATTTTTAAATTTAAATTCATTTTTAAACAACTCATTTTAAGACTCTTTGTAGTTCTTTATCTAGTATTTTATATATGAAATCTTTATTTCTACAAGAAGTTAAAAAATTTTTTAATATTGATAACTTATTTGGTTGAGATTCTAATACCATTTTTATTGTTTTTTCATCCTCTGTAATAAACTCAAATAAATTTGATAGACATTTAGAGCATTTCTGTGGTTTATCCAAATCAACTTTATACTTTAAATACCAACTCATTTATTAAGTTAAAATAATTCAGTAATATTTTTAGCAGTTATATAAGAATCATAATCTTGTGAATAAATTAACATACAACATTGTCTAGATATTAATGACATTGCTTCTTCCATTTTTAACAAATCATCATCGAACATCGTTATATGATTCATAAGCATTTGTTGTTTTGATAGGAAAAACGAAGCGCCTTCACCTTTTAACCACTCATAAAAATCAGATTCATCAAGTTGATTATTATTTGACGTCTTTCTATATTGATTTTTTAAAGATCTCATCATTCTAGAATCTCTCGATGATTTATTAAAAAATGATAATGGGTCATACATCCAAGGTCCTAATTCAGTAAATATAATTGAGTAATGCCCCTTTAAAAATATATCCGTGTTATTAGTATGGCATTCAAAAAAGTTATCGCCTACTTTATAACATACAGCAGATTTTAATTTGTACATAATGCCGTTAATTACAACTTCTTGAGATAATTCTATTGGAGAATCATTTATGATTGATCTGTATCCAGTGTAACACTCCCCACCAAATGTATTTCTGTGCTGTCTACGTTCAACGTAAAAAATTAAAACACCATTAACAATAACTGGCGAACTGATATTCGATATTGTTGGGAAATTATTACCAAAAGCATTAAAATGATTTTCTAAAGGATTACTATAGCCATATCCTATATTAGGTGGTGCAAAAACTACCCTGCCAGTATTTCCATCAAAAGTAAGTGAATTTATGTTACCCGTGTTGATAGAAATTGGACAATTTGAAGTAGTTATCATTTTCATTGGATTAATAACGATATATGGCACAGATTGCAACTGCATTTCATAATTATTCATAAATCCAGGTTTAGGAATCGAAACAAGTGTTGGTTTAAATCCTACTATTGATAATATAGATGCTATTATTTGTTCTTCGTCCGACATAACTTCTGAACAGTTTGTGTGAATTATCTTCATTAAGAATGGATCTATTGACTCTTCATCACTGCAGTAAAAAATTCCCATTCTCATTCTCATTATAAGCTTTTTTATCATTGTATGAATGTTAGCTCTTTGTATTTCGGTAGATATAACATCTGAAATGCCAGTAAATACAATTGGTGCTTCCTCAGTAAGTCTATTAACTAAAAGCATATAATTATCCGGATTAACTTTTTTGTAATCGTAAAGTTGTTGAAGTAAACTATAACTATCCCCATATACAAATGTATTTTCTAACGCCGGTAGTTTTACACCAAAAAGCGACATAAAAATAGGATGAATGTAATATGTTGATTCATTATCTCTATAATGAAATGCTAAATCTGATGAGCTAGACATATCAACAAAGTGGATAGATTGAAATCGAGTTGTTGATAAAAGTATTTCTTGCTTAGTTGGAGAGTATAATTTTTCTAATTCTTTTAGTTGTTCACCTATTTTAGGATGTGCATGCGAATGAATTAAAACTAAGGGATGAGTGCTCTTAACAGACATGCTGGAGTTAGTTAACGAACTTTTAACATGTGCTAAAAGTTCAAATAACTCATTTCTATTAGATCTCATTATATTTAATTTTCGCATTATTGTGAGCATGTCTTGAATTGTAAAATTAGAAACATTGCATTTGTTATCCGTCAAATACCTCGCTATCATATTACCATCTTTTCTTAATTTAACTTGCCAATCATTAGTTGATGCGACTTCATCGATTGGCACAGTTGCTGTATCGGTATCTTCTTTTTTGTTGTTAGGACAAGGCGATGACGTTTCGTTTGTTTTATCTTTACTAAGAATTCTCCTTTTCATTGGTAAAGATCTTTGTCTAGCCCCTGCTGAGATAATATCGTCGGATGTTGCAAAATTTGATAGTGAATTACAAATTGAACAAGATAGTGATGTAATTGGAGTATGAACATGGTTATCCACCAATGATAGAGTTTGATTTTTATATTCTGTATGTAAGTTAACTTTTGAAGTTAGAAATATATCTTTACTAGATTCCATTTATATTCGAAGTTTTTACTAACACTCTATATAGTAATACTAGTTTTTTACATAATTTATTTTGTCAGTTGAAGTTTATTTGCTGTTATTAGTTTTTTACATAATTTATTTTGTCAGTTGAAGTTTATTTGCTGTTATTATCATCCAGTTTGTCTATATTAAATCTCTTTTTTAAGTCATTCATAATTGAGTTTATAGCATCAAAAGTTGTATCTTTAGCAACTGCAATATCTTTAATGAGTGAATCAGAATCATCTTGAAGTGATTTAGTTTTATCTTTTATATCTTTCAGTTCCTTTACTAGTTCATCATGACTAATAGATTTTATTTTTTTTAACGGATCATTTTGTTTTTTATCACTGCATTGATCGTTGAGTTTATTACTGTTGTTTTTAATTTCTATATTTTTGATTTTTAATGTTTTTTTTTCTTCCAATTGATTCATCATCATTTGTTGATATAAATGTTCTTTACTTTTTAACATAGTATCTACATCAGTTAATGTAACAGATGTGTTGTTAATTAAATTTGAAGTAGAAGCTATTTCCTCATCTTTTTTATTCTTTACCGTTGTTTCTAAATCTTTAGTATATTTTTTCATGAAGTCCATTTTATAACCTTTAAAATTGAATTGAAAATATATATATAAAATGGCCGAATCTGATGATATTATCGACGATTATATTTCTGACGAAGATGCGAGCGATGAATTTGAAGAAGAAGAAGAAGAAGAAGAATCGTTAGAAACAAGTGATGTTCCGTCTTTGAAGCAATCTAATTATAAAGTTGGGTCGTCCATATCTATTAATATAGAGGATACCAATCAACAAAATATAACTAACGCAAGCATAATAAGTTCTAAAATATCAGCTATTAAAAAAAGATACACAAGAAGAATAAGTTTATTTGAAATTACTGGTATTATAGCTGAAAGTTATAATCTTTTACAACGTGGAAGAATGCCACTTGTATTAAATTTATCAGATGATACTATGAAACAAAAGATTTTGCATATAGTCATTGAAGAAATTAAAGAGGGCACTTGTCCTATAATAATTGAAAAGAATGGAGAATTGTTATCCGTAACGGATTTTGATAAAAAAGGACTTTATAATCATCTTGATTATATTATCAATATTTGGAAAAAACAAAACAGATTTTAAACATCGTTCTTAAAGAATCTATCCTTTGTGATTTTTATTAACTTTCGTATATCAATTATTTCTCCACAGTACTCTATTTTACTTTCATTAGAAATATTTTGCAATATCTCTATTATTTCTCCTTCCCGTTTAATATATTCTTCTGATAATATAGACTTATACGAAGATTTGTTGTCTGATATAAACCGAATTAATGATTGTAAATTATTTACCCCTTTTTGTTTTATATCATCGATTGATATTTTCGTCTTTATAGAATCCAGCACATCAGTTAATAAGTAGCCCACGTTTGGATTTGTTTTAAATTGTTGATTTATTAAAGAATATAGTTTGGCAACTATATAAATGAATTTTTGAGAATTTAAGTCATTATTTTGAAACAATATAACAGTGCCTAATATTTTCTTAAAAAACATAATATATTTGTTATTATCTACATCTATTGTAGAAATTTCTAAGTCTGATATGCATTTTATTCCGTGAATAATGCTTTCTGTTGAAATGGTGAATATTTCTCTATACTCAGTTAATTTACTTTTATCAGTTACAAGTTTTGAATCAAAAACAGCAATTATTTTTAATAAGTAATTTTCATCAGAAAGAATTTTATTTATTGTCTTGACAATAAAACTATGATTATTTTGCAAAAGTTTATAAGCACTACTTTCACAATTAGTATTTTTAATTTGATTTATAATATCGATAATTTTTTTTGAATCAGATACTATTTCTGCAGTGTCCTGTTTTAAATTTATATACATATTATTAATTGATATAAGCGTTTGTACATTGATTAACATGTCCTTAAATATAGTTTTAAATTTTTCTTTTTCGTTTTCTAATTCTAACTTATTATAAACAGATTTAACTACAGCATCAGATTTTAAAAACCAAAAAGAAAATAATTTGTAGTTACAATACTTCATCATTACGATAATATCTTCATCTTTTGTTGAAACTGATATAGTTGACGAAATTTTCTTTTCTAAAACAGGTACTAAATTCATAAGAATAGAAACATCTGATTCAAAATTTGGATCAGATGTTAACGCATTAGTTTCTTTTTCTAAATATTTTTTACTAATATTAAAAAAATCATAATATAATGTCCTCAGTTTATCCATTTATTGTAAAATATAGTTGTTTAATTTGTCATCGACTTACCGTTATTCGGATAATATTTTTCAAGTTTTGTTAGAATAGAAGGATCCATTTCTATATCTAATCCCAGTTTTAACATGATATTTATTAACCAAGTTTTAGTAAATCTGTCTTTTGATTCTGTAACTACATACTTTAAAAGTGGAGAAAAGTACTTATGTAATTTATACGATGTTGTTATGTTTTTTGTAAATGTTACATAATTTGAAGATAAATCTTGATTAAATTCTTTACCATCAATTAAAAATCCATCTGTTGTCAAATCCATTCCTGAATCAGAACGTATTATTATTGATTTTGTTTGTCTAGGAAACAGATCTTTTATTATTTTATAATTTGGTGGAAAAATTTCTATAATATCTTTATCAAGTGTTTTCTTTACCATAACAATTTCATGGACTATTTCCTGAATTAATATATAAACTTTATTTCCAATATCAAAATAATTCATTGGAAAATATACAATATCGTCAGAAATTAAAATATTTTTATCTTCATTAACAAATGAAATTATATCCGATATTGTACGTATACGTTTAATGTTTTCATTTTCACATGATTTAACTACATGATATCCGCTTGTTTCTTTTAGTCTTTTATTATCTTGTTCAAATATTATTTTTAATCCATCATTAAAAAATGTATCAAAAATAATGGGTAAAAAGGATATCTTTGATGTTGTTACCACCTTTCCAAAGTTGAGTATATATGGATTTATTACGTTACTAATAATATCTTTTTGGTATACACACGATGAAAAAGTTTCAGTGTGTGATTGGTCTTTCAAAAAACAACAAGGTATACACATTTTTTGTAATCTATGAAAGATAGATAAAAATCCTATATTATTATATTTACCCAACGGGTCCAAACATGAAAACATAATTCCATTAGAATTTACAAAAACTTCCTTTGTGCGTGATTTATAAAAATTATCACTTATTTTTTTCATATCTCCCTCTAAAGAAGAGACGATCACTGGCTTTCTATGTTTGTTTTTGGTGTTTTGACATATCCTAGACCAGTATACGGTTTCAACTTTAGTAAAATCAGATGATTGTTGTATATTATTAAACATATTATTAACTGCAATAACTAAAAATGTAAAATATTTTTCAATATTGGGAATGTAGTTTTTAACTTTTATAGATATGTGGTTTTTTGCTAAAATAATTGATATTCTCTTATCTGATGAAAGCAGTATGTTGTTTGTAGCTGTTTCGACAAATATAAAACTGGTTTCTATATCAAGTTTTATTCTAGACGTGATTGGCGTAGATAAAAAAATTTTATACGTTACGTCGCACTTTATACGTTCCATTTGTGTATCAATATCTGATAATAAACTAGTAAATAGTTTGACATTGTTTATCATTATTGTATCTCCATCGCTTGAAAATGCTAATGTGCCATCTGTATCCCAAACTGATAAGTTTATTTTTTCATCAGTTAAAATAAATTTATTCCCTGTGATATTAATAAAAAATTCATCAGATTTGTATAATAAAGATTTATAATTGCATTCCTTTAATTCATTTACCTTTTTTATAATATTTTTTAATCCTGTGAATTTTAAATTTGTCCTAAAAATATTATTGAATTTTGACTCCACCGTCATATCCAAATCCATGGAAGGAAACACGTCAAGTAATCTAGATTCAAATTTTAAAATATTATTATCAACTTCTTCATGGGATCCAAATTCCGGAATTGATGTATCGTTTGCGTTAGCAACCCATATGACTAAAAAATCGCACGCGTCCGGATATATATTATATAAAAATCCATCAGATTTTATCAATGTTTTTCGTTGAGTATGAACGAAAGGGTTAAAAATAGTATTATCGACATAACTATATTCTAAATTGTTTTTATGAGAATAAATAATTATATCATCGTCTATGCTTAGTAAATCACATAAGTATCCTTTTAATTGTCTCATTTTTAAGGTTAATAGTATATGTCTAGTTAATACCTCAGGCGTAGTAATTTTTAAACATGATGTTAAAAAATAATAAATAGGAGATTTATCATCTAACACACCGTACGGTGTTAAATATAAAGCTCTTTTTATTTCTTGTGATTTGCCAACATATAGCACTAATTGCGGACTTACAATATATTTCATTTTATTTATACTGTATAAAGTGAAATTCTATATCTATTACATAAACTTTTGTTATTATGTTTGATCCCGTTCCAGATCTTAATCTGGAAGCTAATATAGAAATTGGTGATGTAAACATCGATAAAATAACATCAGGAGAAGCGAGCGAATCTTTATCGTATGCGTCTAAAAGTAGACGATTGTTTATTCACAAAACTAAGGATGAAGAAAGAAAGTTAGCTCTTAGATTTTTCTTACCTCGTATGTACTTTTTATCATATAAGGAAGTCAACTATTTATTTAGATGTATTGATGCTGTTAAAGATGTCGCCATAACAAAAAAAAATAACATCATTGTCGCACCTTATATGATTTTACTTATAATGGATTCAAAGGGTTATAAATTATCAGAATCAATGATAGAAGTATTTTTCCCTGAACTATACAACGAAAATAGTAAAAAATTTAAGTTTACTTCACAAATATGTAGTATACAAGAAAAGTTAGGTTATAATGTAACAAATTATCATACATATGATTTCGAATCATATTATTCTACTTTTGCATTAACCTTGAGGAAAAATACAGAATCGGATATTATATTTAACACCAGAAATGAGAGCGAATTTATAAGTTCGTTATCTGAAGTAACATATAGATTTTATATTATACTACTTAAAAATAATTTAGTTCAATGGAGTTCAAGTACCGGATCCGTAGTCAATCAAATGATTAATACCGTATTAATAACAATTTACGAAATTTTAAAACAAAAAGAAAGAGACAGAAAGAAATATATATGTCATTTAGCAAATGAGACAAAATTTCCATCTGTCTTTTTGTTAGATCGTATGAACTTATTTGATAAAATTATTTCAGATATAAAATCTACTAATTCTTTTAAAGTAAGCAAGAGAGATAAATCAATTCTTTTAACATATTTTCAATAGTGTAGTAATTGTTTGTTAAGTAATTAATCATCTTCTTTCTTATTTTCATTATTAGAAATTGATAATAGTACTATGCCTAGTATTAAAAACATTATCATGGTAAATATCGATCTTAGTATTACAAACCAAAATGAGTTCGGTCTAAAATTATTTTCACAAAAGTACATAAAAACATGCCTAAAGATATCAACTGCCCCATTAGCTACTTGAAATAAAGCTAACCCTCCAATAGATTTTAAAATTGTAAAATAGCATAGCATTTATTCTATATCAAAAGTGATTAAATTCTTATCATCCATATTAAGATTTTTTTTGATGAAGTTAAAATATGCCCTACTCATTTCAGTGTAATAATTTGTCATATTAATAATTTTAGGTTTAATTATTTCATACGATTTTCTTATTTCATCAGATCCAATTTCTACATCGTCGATAGTTCTTCCATTTCTTTTAATTATGTACAAAATAGCCTTTAATGTTTCTACGGAAATCAAATCTTGATATAACGATTTTGAAAGGTGTGAAAATGATTTTAGTTTTTCTAATAGCTCAACCTTTACATCGTCGCTAAGTTTTGCGTTAAAAATTTTTTCTATAGTGTATATTGATCTTGCTAAGTTTTTAAATATATATGCCATTTGGCAAGATGTTTGTTTGATATCATTAAACTGTTTTTGAGATACGGAAGCCACTGAATTAAATGTTTGTGTTACCAATGATGTGTTTTTTTTAGCATAATTTTCTTTAAGAGCTGTATTAATAAGTTCAGTTATATCATTTGTAGAATCACAATCATCATTTTCGTTAGATATTCCATCTATAAGATCGTTAAATGAAACATTACAAGAACCCGCAACGGTTATTAACTTATCTAATATATTTAATGGCATCGTCTGTAATAACGTATCACCAGATAGTTTATTATCCCTAATAAGATTTTCTAAATTTTTTTTAAAGACGATATTTTCTGGTCCAAATGCGTCCACAGTCATCATTTCATCTGTTGTATTTGCTGAAAATATACCTCTAATATCTATCCTATCCAAGATATTTATTGGTTCAGATATATGAATTAGTGTTGTTTCTTCATCGACATTTTGCGGCAACGAACTATCTTGAGGATTCCCACCATAAAAAAAGTAACTAGGAAAGAAGTTTTTCGTGTAGTAGTATTCTTGATAATTTCGGTAATCGCCTGCAGATACCATAAAATGTAATAAATCATACGACGCCGATATTAGTTTATCAGCTTCTTCCTTTGTACAGCTAGCCTTTACTAACATATTATGTATATATTGTTTTGATACTCTAGGTCTTACGCTTATTATTGTATGATTATTGCTTCCAATTATAGATTTCTTAATACTAACTTTAAATCCCATTGCCGAAAAAAGGAGAATGGAAAAATCTTGATAAGATTCCTTTTTTATTAAGTGTGGTGAACCATTATCGTCAATTTTTAATCCAGCGTATAGCATTAAAACTTCTTTAATTGATAATTCTGGAGATTTATTAGTAATTAATCTAAGTAGTTGAAAAAATCTCATAAAACTATTCTCAGACAGTCCGATATGCATTAGCTTGGACGCTGGTCTTTTTGGAAAATCTTTCTTAGATATAGAGTTTACACTTTCTAACACTTCAGATATTAGTTTTGATACCGTCTTTCCTGATAGTACTCTAGGTAGTAAACATACCCTTAAAGGTGTTTCATTTGATGAGATTACGTCCGAAAGCATTGAGCAATATGTTGTTCCGTTTTTGTTGAACAATGCCATCACATAACATGTTGCATTATAAAAAATGATATCAGATATTTTCTTTGATTTATACTCTTGATAGTCGATACCATCCCAAAATAGTGACAATTTAGATGTTAATTTTCTAGATCTGGATTCCTTTGCCAACAAGTTAAGTAAATTTATATAATAATGATCTCGTCCAGAAATATCAACATATTTAATATTTAAGTTATTGAGTCGATGGATGTATCTGTTATTTGTTGCGGTGTCCAATAAAAGAAGATCGGCATAATCTTGTACTGTTAAATCTGGATATAATTCAATCAGTTTAGGTATTTGATCTTGAAATCGCATAGAAGTTATAATACTAATTAAGTCAGGAATGCTATTCAGTACATAATCTCCATTAAATGTTACGTAGCTGTTTTCGTATTCGTTAGAAAAGTACTGATAATAAATAATAAAGTGTTTCATAGACATTGTAAGAATATCAGTATCCATTGCTCGTTGGAACGGTGGCAATGTGGTATTGTGATCAATTGCGGCATCAAACATTTTAAATTCTTCATCAAACTTATTAATTGATCTTGCATCAATAACCCTTCTTACTCCCAATAGATTTTCGAAATCTCTATAAAACATAACACCGTTAGATTTTCTTACTCTAAAACCTTGTATACTAGTAGACAATACATCCTTATATAAAAGGTTCAATAGGTATGTATTTTCAAAGTTTAAATCTGGGTGTATTTTTTCTTCTACTTGAATATCGAATCCGCCTTCTACTGGTTTAACATATCCGCCTACAAACCTAGAATTATACTCAGGATACTTAAGTCTGTACATTAGTAGTTCTCTAGTAAATTTTGGAAGATCGTTAAAACTATGTGTGTTCACAATTGGATTATTACTTGAATCAAATTTAGGAACTACATCATTAGGAGTAATATATCCAGGATTAATTACATTGTGAAAAAGTTTGTTGCTAGAATATACATCTAATAAATAATTTATTCCTAGTTTTTCTATAGAATTTTGTATTTCATTTTCGTCGCATATCTTTCCATAATCAGGATGTAACAACACATCAAATGGATGCACGTATGACGTTTTCAAATTTTCATCAACTTTATAATCCAAACATAATGATGGCAAAATAGATGATATTACTTTAAAAAGATATTCCGAGTCTTCTAATTGGTCTAATGTGGTAACTGCGTTAATGGGCATCATTTATTTAGTATTAAATGACAACTATACCGGTTACAGATATTCCTAATGATTATAGCGTTACTGCGTTTTCTGAAGATGGATATCCATCAAATAAAAATTATGAAATAACTACTGGTCAACTATCTATTTTAAGAACAGTAAACGATAAATTATTGGCAAAAACGAAATCGCCATCATTTACAGAATTAGAAATAGCCAACTCTCCTGATTTTTTTATACCAGGTGAGGATTCACCTATTACCATAATAGAACACGTCGAATCTCCTCAAACAAAATTTATAGATAATAGTTTAAAATCGGAAATAGTATTAGCAGAAAAACAACGACAACAACGCGTAAATATAAGGGTATCAGGGTTAGAGACGGTGATAGAAAAAGAACCTATAGAGGAGCACATGCGAATAACTTCTATTCCATCTAAAACACCATCGCTTGGTGTTATGTTTGATAAAGATAAAAGGATAAAACTACTAGAGGATGAAATATATGAACTTAAAACACAACAAAGTAAATTACTTAATACTAATAATAATTTAGATAATTTTACAAAATTATTATTTGGGAAGAATATACACCAGTCTTCAGAAATAAATAAAAGAATAGCGATAGTTAACTATGCGAGCCTTACAAAATCTGAATTAACATTGGAAGATTTAGAAATATGTTCTGAAGAAGAAATAGATAAAATTTATAAAGTTGTGAAACAACATAATGATAGTTATAAAAAAAGAATTATCGTGACCCATTTTATAACTATTATAATTATAATATTGGAACAAATATTAGTCAAATTAGGTTTTGAAGAAATGAAAGGTGTGAGCAACGAACTTACATCAGAAATAATTGATTTAAACATAGGAGAAGATTGTGAAGCGATAGCTGTAAAAATTGGTATAGTTAATAGCCCCATTATTAATATTGTTATATTCTTAATTAAAAAACTTATGACAAGGATAAAACTTTGTTAATCCATACCATCGTCGATAGAGTCATCGGCGGTTTTTACTTTATACATTCCACGTTTAATTAACTTTTTTTTTGATTGTGAAATATTTTTGTTTCGTCTACCAGCATTTACTGATTCTATTCCTAACAAGTCTTGATTTATTTCACCTACCATACCTTGAACTTCTAATTTTCCTTCTTTCATGGTTCCGTATACTATTTTTCCGCCATTTGTTACTGCTTGCATTATTTGATCAGTTTCGTTAAATGTATTTCTTTTTTTAAAAACAGCTCCATTATTAACACTTTTCGAAGAACATACGTTACTGCTTCTCGAAGTAGTTTGTTTATTACTGCAAGATTTTGATTTTTTATTTGGTTTTGTCATACAAGCTTTAGTTTTCACATTGCCAGCTTTTACTTCATTCTCATCTGTATTATTAGTAGTATTATCCTCTGGAGTAAGATTTGATTTTTGAGATGCTTGTTCTCCACTAATGTGGGCAAGAATGGTCCTTAGTTGAGGAGTTAGTTTATTTATAGTTTCGATATAATCATCATAACTGCTTCGAGATAATTTTTTATCAGACATGGTTATAAAAAACAAAACAAACAATTTTTTCTTATATTATAATTTATATAATATATATTTTTTTTTATTTACTAGAAAATAATTTATAAAAAGATGTTAAATGTTTAGATTCCAACTTATCAACATATCCAGTTTTTAACTCATTCATTTTTTCATATTTCTCAGCTTCGTGATTTTGATTAGTAGTCGTTTTTTTGTTATATATTCCATAAACTATTAGTCCTATTACTACGACACAAATGATGACTAAAAATATATCACCAATCATTATTTATGTACTAATAAACTTTCTATTATTATACTCCATATAGTATATTTTTTTTAAAAAAATAAACCTAGAACTATTTTTATTGTCCGTTAAGTTCTATAGCACTTGAATTGTATCTACCACCATCTATCAAAACCTTATTAGGAGAACAATATTTGCCCCATGCAGAATAAATAAGCATGCCTATAGTCATCACTATTCCTAAAACAAATGCGATTCCGCTCAAAGCTCGCCATACAGTTATTCCGTTGTTGTTTTTATGTTTAGAAAAATCGACAAATGCAAAAATACATGAAGCTGCAAGTAAAATTATTCCACCAATAAGTGCTGTACTAAAATAGTTACTAATCATACCCATTATGTCCATTTAACTAACAAAAAAATTAAAACACTGAATGTACAAAGTGGAATATAAACCACATAAATAATATTGATTGTATGAAGAAAATAATATTAATTTTAAGTTTGAAGGATAGTTTTAAGTTTCCTAGTAACGTTATTAAAATTATACCAAATAAAATAAGTGGTTCATAATTAGTTATCATTTACTAGCCTAAAAGGTGGGAGTTTATTATCACGTTTAAACATGATAGCTATCTTACCATTCCACTTTGTTCTTTTAATATAAACACAAAATAACATTAATTCTACCAACGATGGATTTATTAATAATATCGTTTTGCTATCATCATTGTTAATTTTGTTAAATTTTTTAAATGTACAAGAATTGTTAGTGGGAAAAGAAATAAATCCTTTTTTATCATATACAGAACACAGAGGCCATTTTTTGTAAACTATTATAGTATTATCGTCTACGAACATTTATATTATTTGTTTTAATTTTTTTACGATTATATATTACTAAAAAATAAAATAAAACAGCGAGACAAATAAAAATAAATGGAAATCCTATTATATTAGGAAGATCTCTTTTTTTTTTAGCTTTGGGTATTCCAGGATCAAGATCACCAATAATATTTTTATTTTTACTACAATCAGATATTAGATCAATTTTAGAGTTTTCTAAAGTTAATGAATTAATATTTATGTTACATCCTATATATTTGCATCTAGATCGTTGAATATCTTGATCATATAATAACCATTTTCTATCTCTAGTTTTATCAGTGCATTCGTGTAACAAACATACTCTAGGGCCTAAATATTTATCAAACGTAATATTATTTGGAGGCGACACACACCAGCAATTTCTGTTTCCTTTATTCTTATTACAGTAACTTAATAATGATATATCTCCAAATGTAAAAAAATTAGGTCTTACAACCCTAATAAACTCAGAACAATATCTTTTATCCATATTATTAGAACATATGTCTGTATATGTACTTAGTGCTATTTTTCTTTTTGTTCTTAGCCATTTTAAACATTGTACATTATCGGGGTTTGATTTGCAAAAAAAAGACATTGCATTATCACAATGCGATGTTTGGTATTCGTTATTTAACTTTTTAGGACAGTTGCCAATACTAGGATTTGTACAACATTTTATAATATCATCATCATTAATAAAATAATCTTTTTTTATAAACTTACATTGAGTACCTTTTAATATGTATTCCTTTGCGGATTCTGGAATTTTATTATCTAAGTAATCCTCAGTAATATCATTTGTATATAATATCATAGATCCTGGTCTAAAACTAAATGATCTACACGAATCCCCCTTAACCAAAATGTACTTTTTTGATATTTCGTCAGAAACAAAGTTGCCACAGTGAGATATTTTTAAATTATCGGTTAAACAAAAATTAGGTAAAATTTCCGATTTTTTGTCATCGTTATATATTTTTTGTTCTTCTAAAAACGTTATAATTTTATTATATTCAGGATAGTTAAAATTTATAAACATATACTTTTTATTATAATCCGCTAAATCTTTTGATACGTTTAAATTTGTTACAGATACAGATCCACCCATTTATTTTATATTTAATTACTCTTCGTAAAAACTTGGTTTCTCTCTGTATTTGTTACCACCTTTAACGTTTTTATAACACGCTTGATCTTGATCTTGATAAGAAATTTTTGAAATATTAAACGCATAAACACAAAATAAAATTGCTAGAATAATGTATGAAATGGTGTAAGCTGTACTAGATATATGTCTAAAAATCAAAGTAATTATAGAAACCGACACGCATAAAATAGACATAATAATTTGTTTGTTATTACCTACCCCTCCGCTGAATTTTTGTATTGATAAACAATACGCAATAACTAAAGAAGGGAATGGTAGCAATGCAGACGCTACAGCAATCATAATAACCGCAATAATTGGAGTAGTTGTGATAGCTAAAACAAATAAAATCAATCCAATCAATGCTCTAATATCACTTCTCATGAGAATATTAGGAAAACGATTTTTTAATGAGTGATGTTTTGATATTTTATATTCTTCTCCTTCTTCATGATGTTCTTTTGGCAAAAATGCTTCTTCTTCTTCTCTAGTAAATAATTCATTATCAGATACGCCTGCACCTGCAGTGAAGTCACTAAACATATTATAATAACTTAAATAACTCATTTATATATTAAAAAATGTCTATGTATTATGAGATAGACTATAAACTATATAGTCAGATAAAAAGAATTATAGACGACCAACAATTATTTCTATTTAACGATAAAAAAGAATTTGTAGAAGTTGAACCAAAATCTATGTTTAAATTTATATTACCTATTGGTTTATTCTCATCTGAAAGTTTTCCATTACCATCGAAAATCTTTGCAGAGAAAAAGAATTACATTATTAATTTAGATGCAATAACTTTGCCTAATTTATATCCTCTTCAAAAAAAAGTTGTATTTAAAGTATTATCTTTAATGCGTGAAAAACGATTACAAGGAAGACCGATGTATATAACATTACATCTATCTTGTGGATTTGGAAAAACAATAACCGCTTGTTATTTAATGTTGAGACATAAGAGAAAAACTGTAATTTGTTTACCTAATAAGATGTTAGCATCTCAATGGAAAACAGTTATTGAATCCACTAAGCTGAGTTATATAATTTCCTTAGATGGTGTTAAAAAGTTAATGGATAAGTTAAAATTTGAAAACGTTGACGTGTTGATAATTATTAATAGGCATTTATCTAATGAAGATTTTTGCAAAAAAATATATAGCGATTATGATACTTTTATATTAGACGAATCACATATGTATAATTTAATGAACAATTCTATACTAACTAGATTTTTAACATTTTATCCACCAAAAATATGCTATTTTTTAACAGCTACTCCTAGAAAATATAACAGGCTTTATTGTAATGAAATCGTTAACATTTTAAAAATTACTGATATGGTAAAGACAATTAAAGTTGTTGAATACTTTTTTGAAAATTATTCGTCTGTATCTATACGTAAAATGTTAAAGAGAAAACTTGACAATGATAAGTATCATATATACACTGAAAAAATACTATCAGAAGATTCTCCAAGAAATGAACTAATATTAAAAACAATTTTTGAAAATTTTAAAAATGATATCATAAAAAGAACTATAGTTGTAACAAAACTAAGATCGCATATGATGTTATTATATAGCGAGCTAAATAAAATTTTGGGAAACGAGTTGGTATTTTTAGGGGATGCAAAAAACAAAAGCTCATATGACGTAGTAAAACGTATAAGGGAAAAAGAAAAATTTATATTTGTTTCAACTATTAATTATTCTGGTACTGGATTAGATATACCAACATTAGACTCTCTAATTTTGTGTAACGTAGTATTAAACACCATTCAAGTTGAACAACTATTTGGAAGAATATGTAGAGAAAGTGACCATCAAAAACGAATTGTTTTTCTATTTCCAACTACGTCAATAAAAGAAATTAGACACTTAGTTGGGTTTTTCACACAAAAAATAATATCGTTTTCAATGGAAAAATTAGGATTTGAAAAAGAGATAGAAGGTAACGATAGAGGAAAAAAAGAGCTTGCGTTGTGTAAAGCACTCAATTTACAAACCCATTAAGCATTCTAAGTTCAGACCCACATGCAGAACATGTTAATATATTTCCCTTTCCGCCAACTTTGTATTCATTTAATGATACCTTTGTTATATCTGAAACTTTTACTAATTTTGAATAACACGATGAGCATGTCATGCATTCTTCATCTTGTACAGTTGTTTTTGGTTTTCGTTTCTTTCTGCGTTTATTACCCGCACTATCTTCCATTTATGTAGTAAAAAATAAATTTAAGTAAGCATTTGAACAGGATCTCGAAAAGTTTACTATATCATTATCATTATTTTCACACGATAAATAATTATTTTTATTTGCTTTTTTTATTTCCGAACACCTTAGAGGTTTTTGTTTATTATTATCAAGTAACATTTTTGCAGATAATCCTGAAATATTAAAATCATAATAAAAAAGGTTATTGTCTACACAAATAAAATCCTTTTTTATGTTTTTATATTTTGTATATGCGTCGTATTCACGTTTCAACTTTTCAGAAACAGTAGGTATGATAATATTAAAAATAAGAATGAAATAACATAAAATTAGAAAAAGGCTTATCATGACTACAGAAAGCGATTTAAAAAACTTAAAAGAGTTACTAAAATTAAAGAAATGTATAAATATAGCAGACCAAAAAACAAGGGAAAAATATAATTCTATCGTTGAATGGGCATCGAATAAGTTTTGGAAAATAAACACTCATAAGGTTATCGATGCCGAAACTTCTATTAGTGATTATTACAATACTTCAAAAAACGAATCATTTATTTTAAAAAATGGTAAATATGTATTTTTACCCATGTGTTTTGGTAATATATTTATTTATTTTAAAGGAACTATGATGGAACTCGGATCTGGAAATATACAATATATTGATATTAAAATTAAAAAAAAGATAGATAAGTTATTAAAAGAAAACTTAGATATAGAATTTCTTAGATTTGTGTACTTTAAAGGAGAGTGGATATTAGAAGATTGTTTTTCAAAATGTAAATGCCCAATAAATTTTTTAAAAACGGCATCAACTAATGGTCTAAACACTGTTCCATACATATTATTAAATACTAAAGAAATAATATTTACAGAAGATGATTATGAGAATATAGATTCTTATTTTACATTTTATATAAAAGAACCTTTTAAAATTAGTTCGTTGTGTTATATAGAAGAAGGGAACAACGAAAGAAATATAATAGATTTCTATAAAAAAACATATGTTTTTGTCGAATCTATAAAAATAGAAAATATACACAACAATTATTATATTCCTAAAATAGTGACAAAATCAAAAAATGTGGTGTTAGTAAGGGATATAAATCACTTAATAGACTCTAAAATAAAAATAAATTCATTCATAAGCGTTAAGAAAAAAAAATACTTTTTAATACTAGACAATAATTCTATTTTTTCATTAGAGACACCCTCAGAAGCATTTTGTAGAATTATGAAATATATAGGAAATGAATTTTATGTTAACGGTAAATATCTATCAAAGGTTAGTAATGATATAGAAATAAAACATTTATCTAGTAAATTGGGATTATTATCATCCACATTAAGTGAACTTATTACTGAGATTAGCTTGTGTGATAAATTAAGTGAAAAAATAAAAAATCAATCAGTGTTTGAATTTGTAATGGAATGTTTGGAATATCCAAAGGCAGATTTTCTAACATTAGTTAATAATATGTCATTTGATGTAAAAAATGGTATTGTTTTAAATTTTAAGTTAGAAAATATAAATTGTTTAGATAATCCAAATATATCTGCAATATATGGTAACTTTAATAAGTTTATATCATTATTTAATATTTTTATAGATGTCAAACAATCATTGTTTAAAGCATATTAAAAAAGAGGTTATTTGTGCATTTGATATAGGTGCTAAAAATCCAGCTAGGACAGTATTAGAAATAGAAAGTAATAATATAAAAATAATTGATATATCGAAATTAGATTGGTCATGTAAATGGGAAGAACAAGTTGCTAAAGATTTATTTCAACATAACTATAATTATGTGCTATTGGAACGACAATCAAAAAGATCGCCATATATTAAATTTATCCATTTTATTAAAGGGTTGTTATACAATTCTGATACAAAAGTAATATCAGTATCGCCGGTAATGGTTGGTTACTCATATAAAGATAGAAAAAAAAAATCTACAGAATCTTTTTTAAATTGGATGAAAGTATTTGGATTAGTAAATTCTATCCCAAAGGGAAAAAAAATAGATGATGTAGCAGATAGTTTTAATATCGCACTAAGGTTTGTTTTAGATAAATGGGATATTAATTATATTCCGTATAAAAAAATAAAAAAAATAGTTAAAAAACCATTTAAAAATAATAATGGATGAATTATTTACATTTTTACATAAAATAGAGAATGAGTATTCTAGAACTATATTTAATTTTCATCTTATAAGTTATAAAGACAAGTACAAAATATATGATATAATGAAAGAAAAAATATCAATCGAAAACATTTTTAACAATATAGCATTAACTGATGAAATTAAGAATCATATTAAAAAGTTGGTTTATTGTGATATCCATTTAACAAAGCATATTATAAACAATATAGTTTATCCTAAATATGATAATTTAAATCAAAACAATAATTTAAAATTTTCTCAATTTTTTGATATCAATAAAGATGATGACTGTATTAGTTTAAGAACTGCTGAAATATTTGATAATGATAAATCATCATTAATATCTTATATAAAAACCACCAATAAAAAAAAAAAAGTTGATTATGGCGAGATAAAAAAAACAGTGCATGGGAACACAAAAAATTATTCAAATTATTTTTCCGGCAAAAAATCTGACGATTATCTTGTTACGACGATAAATACTAAAGAATCACAGCCATGGATAAAAACAATTTCTAAGCGTATGCGAATAGATATTAAAAAAAATGCAATCATAACAAAGGGAAAAAGTTCAATTTTACAAACAATAGAAATAGTATTTGTAAATAGAACATGTATAAAAATTTTTAAAGATTCGACGATGCATATTATATTATCAAAAGAAAAGGATGAAATTGGTTGTGTTGGAATTATAAATAAAATATTTCAAGTATATAAAATATTGTTTTGTTTACTTTACGATATTTCAAAAAATGAAAATTTTAAAAATGCATTTGATAATTCTGTATCTATTTTAAACTTAACTAAGTTTGATGATAAGATTGCAAAAATAAAAACAGTAAAAAATGAATACGGGTTTGAAAACTTTAAAATTGGAATGTTTAATTTAACATATAACAAACCTATTTTGCATACAGTATTTCCGTCGTTGTTAGATTACGAAAACAAAATAAAATTTTTTAAAGGAAAAAAACTAAATATAGTTGCTCTTAGATCATTAGATGAATGTATTCAATATGTGTCGTTAGCAAATAAAATATTAAAAAAGATGGCAGATCGGTCCGATATTCTAAATAATTTAAATATAGAATCTGAAAGTATAGAAATACTAAAAAAAATATTGTTTAATTAATTTAAAGTGAAAAAAAACACTATTTTGAAAATGGACCAAAGATTAGGATACAAGTTTCTTTCTCCTGATCCAAAAATCGGAGTGTTTTATAGACCGTTACATTTTCAATATAAATCGTATTCGAACTTTATCAATTACAGATTACAAGAAATTTTATCGGTTAAAAGAACTCTATTGTCGTTTAAAAATGATACTGAAAAGATTATAATCGAAATTGATAATATAAAAGTGACACCGCCAGAGTATTCACCTATTATAGCTAGTATAAAAGGAAAGAGTTATGATGCATTAGTAACATTTACTGTGCACATTTATAAGGAAGTAATGACAAAAGATGGAACTACTATAACCAAAATTAGTAGTTATGAAGGTAACGATTCTCATTTAATTAAAATACCATTATTAATAGGTTATGGAAATAAAAACCCATTGGATACAGCAAAATATGTTGTTCCAAATGTTATCGGTGGTGTATTTATCAATAAACAATCTGTTGAAAAAGTAGGAATAAATTTAGTAGAAAAATTAACAACATGGCCAAAATTTAGAGTTATTAAACCAAACGCGTTTACTTTTTCTTTTTCATCAGTTTCGCCGGCACATATACTACCGACTAAGTATAGGCATTATAAAATACTAATGGACTTATCTCAATTAGATAATTGTTATATTTCATCGACTAAAACATTCATAACCGTTAATATAATTTTATTGGTTCAATATTTATCTAGAGTAAGTTTAAGCTTTATAAAACACAGTTTATCTTATGATATGCCTTCAGAAATTTCGTATTTGGTTAACACAATAATAGAAAGTACAAAAGAATTGATAAAAACGATAAACGATTTTGACATTGACACATACATTAATGATTTAATAATATCTGAATATAATAAACAAAAATCGCAATTAATTTTGGAAGAATTTAAACATGAAATGATAAATAATTTTCTACCACATATGAATGACACGCCTAACCAACTAAAAGGTTTTTATATTATGTCATTATTAAGAAAATTTATTTATTGCATTTACTACACGTCTAGATATCCTGATCGTGATTCGATGGTTTGTCATAGAGTTTTAACATATGGAAAATACTTTGAAATTTTAGCACATGACGAGTTAGAAAATTACATAGGAAATATACGAACAGACATTATCAATAATCATAAAAATAGAGGAACTTATTCAGTAAACATACATGTATTAACAACACCGGGATTTAATCATGCGTTTTCTGGATTATTAAGTGGAAAGTTCAAAAAAACAGACGGTAGTTACAGGACTCACTCACATTATTCTTGGATGCAAAATATATCCATTCCAAGAAGTGTCGGATATTACCCGGATCAGGTTAAAATTTCTAAAATGTTTTCAGTAAGAAAATATCATCCGAGTCAATATGCATACTTTTGTCCGTCAGATGTTCCTGAAAGAGGTCCACAAGTAGGACTAGTGTCACAATTATCTGTTCTTACATCTATTACCAATATATGCACGAATGAATATTTAGAATTAGAAAAAAAAATTTGTAATTATATACGATCATATAACCATAACGATATAAGTTATTTTGAAACTGGGTATTATATAACATTAGAAAATTCATTAATTGCTTGTCTTAATCCAAATTTGGTCGATGATTTTGTTATTGATTTTAGAAGAAAAAAAAGAATGAACTATTTTGGCAACTTAGAAATAGGAATTACGTTGGTTAATGATCATATGAACGAAATTAGAATCAACATAGGAGGAGGTAGATTAATTAGACCATTTTTAGTTATTGATAATGGTAATCTTATCATGGATGAAATATTTTCAGAACTCGAATTTAAAATAGATGATATGACATTTTCTGATATACAAAAAGAATTTCCACATGTTATTGAAATAGTTGACATTGAACAATTTACATTTAGCAATGTATGTGAATCAGTGCAAAAATTTAGAGCTCTTCCCAAATCTGAAAAATGTAAATATCATTTGTGTGACTTTCCTGCCGAATTTAAAGATGGGTATGTTGCATCTTCACTGGTTGGTATAAATCATAATTCTGGCCCTAGAGCTATTTTGGGATGCGCGCAAGCAAAGCAGGCCATATCTTGTTTAAGTTCAGATATACGAAATAAAATAGATAATGGTATCCATTTGATTTATCCAGAAAGACCTATTGTTATTAGCAAAGCATTAGAAACATCTAAAATCGCGGTAAATTGCTTCGGGCAACATGTTACTATTGCATTAATGTCTTATAAAGGAATAAATCAAGAAGATGGTATTATTATTAAAAAACAGTTTGTTGAACGCGGAGGTTTAGACATAATAACTGCTAAAAAACATCAGGTAGAAATTCCATTAGAGAATTTTAATAATAAAGAAAGAGTTAAATCTACAGCTTACTCAAAGTTAGAAAGTAATGGCTTAGTAAGACTAAATGCGTTTTTAGAATCAGGAGACGCTATTGCCAGAAACATATCGTCGCGAACATTAGAGGATGATTTTGTTCAAGATAACCAAATTAGTTTTGACATATCTGATAGATATACTGATATGTACCAATCACGGGTTGAGAGAGTTCAGGTAGATCTAACGGACAAAGTAAAAGTTAGGGTTTTGACAATGAAAGAAAGACGGCCAGTATTAGGTGATAAATTTACTAGTAGAACTAGTCAAAAAGGAACAGTAGCGTATATAGCAGATGAAACAGAATTACCATATGATGAAAATGGAATTAAACCAGATGTTATAATTAATTCTACATCTATATTTTCAAGAAAAACGGTTTCTATGTTAATTGAAGTTATATTAACATCAGCGTACGCAAGTAAGCCATATAATAATGATGGTCAAAACAGACCAATATGCTTCCCTAGTAGCAATGAAACCAGCATCGATACATATTTGGATTTTGCCAAAAGATGTCACAGAGATAGATATCCAAGTTTATCAGACGATGATATAAATGATAAAATGTTTTGTGATACAATTTTATATGATCCCGAAACAGATAAACCGTATTCGTCGAAAATATTTATGGGTCCGATATATTATCTTAGATTGCGTCACCTAACTCAGGATAAGGCAACAGTTAGATGCCGGGGCAAAAAAACAAAGCTAATACGTCAAGCTAACGAAGGGCGTCGACGAGGCGGCGGTATTAAATTTGGAGAAATGGAAAGAGATTGTTTAATCGCACACGGTGCAGCAAATACGATAACAGAAGTATTAAAAGATTCGGAAGAGGACTATCAAGATGTGTATGTCTGTGAAAACTGTGGAGATATAACAGCACAGATACAAGGAAATAAAGTTTGTATTAGGTGCTCTAAACAAAATTTATCTACTATACTAACCAAAGTTGATACAACACATGTGGCAAAGGTTTTCATAACTCAAATGAATGCAAGGGGTGTGAAGGTTAAACTGGAGTTTGAAAAACGGAACCCATTATTTTATAAACCTCTGGATGTGGTAGATTTAAGTCCTAATTTTTTGTAAAATGTATACTCACTCATAGTGTTGTACTTCGGCCTGTTTGAAAATCTACCTTTTTTGTTAAAACAATCATATTTTTTCTAATAGTTTCTATATGATTTTCTAATCTTTTAATAATATTTTTTAGTGAATTGCATTCTTTTACTGACGTTTGAAATAGTTTTTCTAATACCAACAGTCTTAAAACAAAATCTTTTATTTGCATTTCTTCGTTACTAATGAACGAATCTTTTAAAATGTCAGAAATTTCATCGTCGTCGATAGAAATATAATTGCTATATCTTTCATTAATTATTTTCTTAATATCATTTTTTATTTTTATCATATTGGGTTCTTCCTCACTTAAGTCCAAAAGTTTATCTTCATAATGCCCGTGTTCGCCACTAGTTTTCTCTCTGTGATTTATATTTCTTTCATTGGTTTCATCATCATCGCCTGGAAAGATTGATAAAGCTCTGTCCATTTAAAACATAGATTTTAAAAAAATACACTCTTTACTTTTTATACCATTTTGTAATATACATGGATTTTTAATATCTGAATGAGTTGATTGCGAAAATGTATAATCGATACACGACTCTAAATTTTTAAACTTTCTTATTTCTGATCCATTAGATTTAAACCCAAACATTGATACAGAAACATAATCATTGTCAAGTTTAACACACCTCCACTTTTGTTTAGGATCATAAATGTCATCGTTTGGATCTTTTATAGTTCTATCAAGAGATAAAGTATTTACTGATTTAGAAAACTCAAACGCTGAATGAGCTGAATTAAATTCTTTTATGTTGTCATAATTTAGATATATTGAGTATAGTTGAAAGATAATTATACAAACGGCTACTGTGGATAAAATAATAAAAAAAATTGTTATTGCGTTCATTTACACTTCTAAATAATTAGCGATTTTTTTAAATGCGTCTGCTAGAGCTTTCAAAAATGTATTTATATCTAGTTTTGTCATATCAAACCTATGGTAACTATTTAATCTAGAATTGCATCCAAAAAAGTTTACCACTGTCATAGTTAGCAAATCATTTACAAACAGCATTCCAGAAACAGGATTATAAGAAGAACTTGTTGAATTATCTAGAACCGATGTTTCTAACTTATGCGAATGTACATCTGAAACTAGTGATCCCTCTAAAAAGTCTAATATTTCTTTCATATGTGATTTAAAATCGATCCATGGATAATGTTCTACATAAAATGTTAATCTACCTAATGGTGCTGTAACTATAAACGGTATAATACTTTCAAAGCTTGTCTTTAACGTAGGTTTGATGTTGTCGTATTCTTTATTAAATGTTTCAATATTTATAAATGTTAATCCCTCTATTATTTTTTCTGTAATTTGAATGTTCGAACTAAACGCTATAATTTCAATAACTTGTAAATTTTTTATTCTATAATCTTGAAAAGTGTGTAAAAGTGTTTCATTTAATTCAATAGGTTTATCTATTTTTATAAATTTATAATTTTCTAAATCTATAATATAATAGTCGAAATCTCCCAATGTAATAATTCTTTTTTTCTCTGTTTGGGATTTTTTTAAATATACACAAAAACCTACTTCTTTTCTTAAAATTTTCGATGTGTAATTATAAAATATCTTTCTTGTAGGCATATGCTCTATAGAAGTTAACCATTCTTCATTGACGGCAGTTGTATTATTTGATAAAACCACACCTGTAGTTAATAACGGCCATCTAACTCTATTGTTAAATCCATGTTTTATAAATGTTGCCAAATCTGGATTTAAATCAATAACGATTACCTTCTCTTCTCGGAACATAACTTAATTGCTGATTTTCACTTTTAAAATTTTTTTTTGACTTTTTATTTAATGATAAAACTCTAAGATTTATCTGAGATATTATTTCTTTAACTGTTGATAGAGTGGCAGTGAAATCATTACAATCTGATTTAGTTGATAAATTTGTTATTAAATGAATAAAATTAGATTCATCTATATCTTCTTCACTCATTTAAAAACTTATAAAGCACAAAAATACTTATTCTGTATTATCATCAATAGATGGTATAAATATTAATATTAATAGTTTACAAATAGTACCTTTTCCATCAACTATATAACATTTGGATAATATTTTAATTGCTTCATTTAAAAAGTTATTCATTTAGTATTATTAAATATTATTTACATTATTTTTCATACGATGATAATATTTTCGTTAAGTTTTTCTTCATATGTGAAAATTGTTTTAAAAGGATACTTGGATCAACTATATGTCTAATTACAGATTCATCAGCAGTATCATAACATATTCTTTGTTCGCCATTGCTAAAAACAGAATCTTCTATAATTAATACTCTTCTCTTAGAAACATTAACTGCCCTCATCAAACTTACTGATTTTAACAAATCTTTTTTTGACCCAGTAATAGACATAGATCTCAACATATTTTCTATATCAGAATCTGACACGTTACAACAACAAAAATGTGTTATACTTGTTCTACCATTAATTGGAACATGTTTATATGTTTGACATAGTAATATTATTGAAGTATTAAGATGTCTTCCATGATTTAAAAAATCCAACAAACAACTTGATTTTGTTTGTTTATCCCCTAAATCGTCCAAAATTAATAAAAACATATCTGCTTTTTTATTTCCCTTTGTTTCGACATATTTTTCGATTTTTTCTTTTGTTGTAATTAATGCATAATCAAGTTCTTCTGGAGTCGTCACTCTATTGACATGATCAGGCCATATATAACTATCATATGCAGAATTGTAAACAGGAGTAAATAAAAATATATGTTTGTATTTTTCAACTAATGTACTAAATAAAGATAAAAGATATGCAGTTTTTCCTGATCCGGATCCACCAACTAATGCTATCCTAAATGGATTTGTTATTAAACTATTTCTTAAAAAATGTTTTTCCTTAAACCTATTCATCATATTTATAATTACTGTTATTAGTTAATTTGGTATTATTAATAATAAATGTAGAAAAATAAGCCACAAATGTCGAGACAATTAAAACTTGTAACTATAAAAAAAATACGTTACAAATAAAAGAACTTTAAAATATGTTAGTTGATATTCCAAAGAGTGGAACTGAAACAGATTATGATGAAAGTAATAATTTTACAGCATTCGCAGGTTCCACTATATACGGATATGGTTTAAAATCAAAAAAAAATATAAAAAAAAAAGTAAAATTAATTAATTTCTGTATAAAAATATCAATTATGACATCAATGGTTTCGTTAATTACAATAACAATTCTTTTAGCATTTTTTAATAATACATGTGAATTAAATCAATTTAAGGAACACAAACCGTACTTTTTAAAAAATCCAAATCCTACTACATATAGTGACGACGATACTGAATCTGAGTTAAATGTTTATAGATCATGTAAAGGTATTGTTTATAGCGGATACTGTTACACGTTTAACTCAGAACCTAAAAGTTTTAATGATGCATACGATGATTGTGAAAAAAAAAATAGCGAATTACCATCAAATAATTTAATGAATGATTGGATAAGTGACTACTTAGATGGGACGTGGGGCGAAGACGGTAACGTACTTTTTAAAGAAAAAAATCAAGAACTTGAAGCTATAGATATAAGCGATGAGATGAGAAGCTATTACTGTGTAAGATCTTTTTTTTAAAAAAAAAAATAAATTATTATATTACTATAAATGAAGTCATTAAATAGACAAACCATAAATAAGATTAAACGTGCTTCTGCACCTACTGCTATTTTTATGCTAGTATCAACTATTGTTAGTGGTATAGGCACCGCAATACGATACAAAGATGAACTATTTCCTAGTGCATGTAATAAAGGATGGGTACCATATGATGATAGCTGTTACTTAGATTCGAGGCTTCAACTTTCATTATATGGTGGTGTAATGTTATGCAATAAGTATAATGCAAAGATACCTAATGTTAGTATTAGACATTTAAAAGTAATCTCGTTAACTTATGGTAGACAATTTTGGTATGGACTTGTAAAAAAAAAAAATAATATATGGGTAGATGTTAATAGTAATAGCACTGTAGATATGAATAAAAACACGGAACTTAGTAATATAAAAAAAAGTAGTAAAGGAGATATTAATGCATGTTATGTTTATAATTTTGGCCAATTTAAAAATGTGTCGTGTAACTACGTAAGTTATATAATCTGTGTTAAAAGGTTATATAATTGAAAAATGAGATTACCTAAATAGAGGAAAATGGATTTTGACTTCATTTTCGACAAAGACGAGGATGATATTTATACGTTAATAACAACTTTAGGTGTATTAAAAATAAAAAAGAAAGAAATATCAAAAGTTTGTAGTGAGCTAGGCATTAATCTTATAGAAACATTAGGACCTTATAACGTAGTATCTTTAAATATACACCCATTTCCTAACAATTTCATAGAACAATCAAATTTGATTAATTGTTATATATCTTATAATGGGACACTGTTTCACTGTTCTAAAGATGAAAGACTGAGTATCCCAATAAATGGCTTGTATAGAGGGTTTTACTCTAACAATAGTTTTATATTTTCTTTTGATAAAGAAAATTATGGTAAGTTACTAATTGATGAAAAAGAACAATATTTTTACCTAGGGACAGCATATGATATAGTTAATTCAAATATCATCGAAGTGTATAATTTATACAGGAAAGGAGATTACAATTTTATTATAAATCCATCGGATAATTTTTTAGAAATGATAGCTAATCAATCAAAAATGTGCTTAACTGATAAAAGTGGGTGGTGTATTGTGGATATAAAAAATGAAATAGAATATTAATTATGAAAATGTAATAGTTTTGTGTATATTATAACTATGATTAACAGAATAAATTTATCGTATGGAATTGTTAAATATATTAAAGCTTTGTTATTAAAAGAAAATATATCAGAATCAGATAAAAGTATTATTAAAACAATAATAAATGAATCCATTTATCCACATAATTATGTTTATAATATTTTAGATTTTAATTCGCTAACGATAAATTTTAACACATGTGAAGAACGTAACATAATAGATTTTAAAGATGTCAATATAAAACCACAAAGGTTCATAAGCTATTATAAAGATTATATATTAGAATCTAGCGAATATATGTTTATAGTTTGTTTAAAAGGAGAAACCATTATAAAATGTTATAATAACAATGTAATTTCATCTAACAAAGTACAAAAAGGAGAAGCTTTTACATTGAATATTAAAACTAGATATAGCACAATAACAAAAAATAGAGATCTACATCTAGCGATTATAACATATACCACAATTTATCCATTAATATATTATAAAAATATTGTTTTTTCAAAGGATAGTTCATTATATAACATTTTTTCCGGATACAAATTTGCACTTTTTAGAATTTCAAGCGACGATGATAAAGTTTTAGAAGATATAATAATTCTGAATGGGAGGTACTATTACAGCAATTCAATGGAAAAGGTTAATATAATTAATATAAAGACTCTATTGGATAAGTACAATATAAAAATTAATAGTTTTACACAAATTGATTATATTCCTAAAAGCTCACACAAGGCATGTGTAGAGGCATTAGAGTTGTCATTAGATGATGTGACAGTCAGACAAATTTACGATGGTACTTCTTTTAAAGATGTATGCTCTATATCTGGTATCAAATATAAAATAGTAATTTCGTATTGTGTTCTTTGTTATAATTAATCTTCTTCTTTTTTTAAAAAAAATACTGAACAAAAAAGCTTTTTTACATGGTTAAATAATGGGAATAGTATCTGTTGTATACGTCGTAGTACCATTTTCATTTATTGTTTTACTTTCATATATATTTTTTGAATACAAAAATGTTATTAAAAAAATGTTATTTAAAAAAAAAGGGAAAAATCAAAAAAGAACATGTGTTAGACTTAATTCAATCACTTATTCAACAAATAGTATAGAATCCACTATATCAGAAAGTACTTGGTCAAATTGTAGTAATGATACATTTGTAAAAAATGAAAAAGAAAATGTAGAGATTGTTGAAATTAAACGTTGTGATAATGAATTAATTGAAGAAGGTAATAATAACGTTTTAGAAAATGGATGTACCACAAATACAGGTGAAGAAAATTTAATTTGGGATGATAACAACGTTTATGATTTACCGCCTAACGATTTGAGTTGTAACAACGATTGTGTTTATACATTACCGGACGACAATGTTTCAAACATAGAGGAAAAAATAACTAAGTTAATGCACAAAAATAATTCCGAGTCAAACTATTATAATTGTTGTTAAATTTTTTAACATGAAAAATAAAATATTCAGATCGATTCCGTTTTTTGATATCATTAAAATAAAAACGCAAGTTTTAGATTTACTTTGTATGAACATAACAAAATACTATAATGAAGAACATGTAAACATTATAGTAAAAGAGATAGAACAATATATTAACGAAAAGGTATTTGTAAAAAATTTAATATCGATACGATGTTTTAATAAAGAAGGATGTAATAAAAATAGACAAAATTTTAATACATCTTTTTCTAAAGCACTTATATGTATCAAATCGGCAAAAAAAGGTGGGGTAATGGTTATTTTTAATAAAGCTACAAAAAATAAAAAAGTAGTATATCCTAGTTCTAATCATATAATATTGTTAAATCCATTAAGTTATTACACCATTTCTCAAGTAAATAGGGGTTCGGTAATAATGATGGTATTGGATATTGATATTCCAAGTATGAGAATAATGTATAAAAGCATAAATAATGTAAAATATTCTAATGTATTAGCGTTACTATGCCCGTTTTTTAAAAATGAGTTTGTTTTTGCACTAAAGCAATTAATTGATATAAATAATAATCAGGTCTTATGTGAACAAATAGTTATTAACAGGGAATACTATACAGTTGTTTCGACAAATTTAAAAAAATTCTATATACCATCTATATGTTTTGGAAAAAAAATAATAGATTTTAATTATGGATATGATGATTTTAATGAAGACATAATAAATAAAATAATAAACAGTGATACTCCATTTGATTTTATATCACCACAGAAAAGTATTATTGACGCGTCGTTAGTTTATGAAAAAGTTATATATGGAAAAATAAATGTTTAATTTTTATTCTATAGAAAAAAGTAAGTTTTCGTCTTCTATTATATGAAGTTTTCTGTATTTAATACTAGTATTTAAAGAAAAGATAAACAATGATAATAAACTAATAAATTCAAAAATGATAACCAATAATAGTTTATAAACTATGACTTTGCCATATATTTCATTATAACACCCTATAAGGGACATGGTAAACATAATTAGTGATATTATATATGATATAAAATGTAACAAAAAAACTGTTATCAGCGATAATGTGTTTTTAAATTTTTTCTTAAAAAATATTATTTGTAATATTATAGATATAAAAATAGATACTTGTATAAATGAAGTTCCTAAAATACGTATAAAAATATTGTTTGTGTTTAATCCTATCATGTATTGTCCTAGTAACATGATCAGAGTTATAACAAATCCTATGTAATAGTATAGATAAATTATTTTTTTATCTTTTTTAAAACTAAGTTTAAACTTAATTGTTGTAGCTATTATTTTACCCCATAGTAGTATGACATAAATAGAAAGCAATATAACAACTGCATTTATTTCTTTTTCATTTAACCATTTTATAATATATTTCAATTGTATAGTTTCTTTTAAGAGTATATTATCGTTATTAATTACATAACAGGTATAATTTCCGTATAAGTATTTTGAAAAATCCAATATCAGTGTTTGAGTATTAGTATTATTTTTTTTAAAAAGTGGGTAGTTATTAAAAAACCAATTAATTGTAACATTTCCATTATACTTAAAATGATTAATGTCACAGTCGATGATAATTGTTTTATTACATTTTGAATAATTTATTGAAGAAACTTTGTTTTCTGGTATTGAAAAAGATGAATTAATATAAATTGTCAAAAAAAGAAATATTATGTTTACTTTCTTTTTTTTAAAATAAAACATATTTAAAATACTATAAAGTTTCTTAACTTATACAAAAAAACGTACTATAAATAGTATTAATTGGGAACTAATTAATTATTTTTTTTTAAAAATAAATATGAAAGAGGTTTATACAAATTTTAATATAAGTTTAAATACTACAAATCAAAAAATTGAAGTAATGGGATCAGTTCCTACAATTGATGGAAAAGATCCGTCTATAGATATTAGAATTGTTAGTAAACCAAATAAAGAAAAAAATAATTTTGATAATACTAACGAAAATATTTTTTTAGATGATGATAATACTAACGACTATGATGAAAATTTTATAAAACCTAATAAAGTTTCGTATGATGAAACAAAAAAAGGAAAAGGGGTCGACATTGAACAAACCGATAATGAAAAACAACTATTAAGTGATTTTAGTGATATTCTAAACAATGATGAAAAAAATCACGATAAAGATAAAAAAAAGGTTGTAGCGTAAATTAAGCAAAATATTGTATACTTTATGTATACGTTTTAATATTGAATTAATATATAAGTTTATAATTATGGAAGAACTTGAATGTATGTCGATAAAAATAACTTCCGAAAATGGGAGCACCATTTCCATATACTGTGAATCCAAAACTAAAATATCATTAGATATAACAACAAAAAAAAATAACATAAAACCTATTACAACAAAAAAATCTATTAAAAAAATAGAAAAAAATGATGATGATGATTTATGCGCAGACCAAGAAAATGCAATGGAAATAGATGGGGAGCATACAACTGATAATAACAAACTATAGTTATAGTTAGTTTGGTTTTTAAATAACAAATATTGACAATCACTGTTTTAATCATATTAAATGATTAGGAAATATTATGAATTTAATAAAACAGTTTCAAAAGCGGTCTGTGTTTTGAAAGGATATAAACTTCATGGTGTTATTAATTTTGATCAGTTACAAAATGGAATAGTAATTATATCAGGCGTTGTGCTCGGATTACCGGAAGGTAATCATGGATTACATGTACACGAGTTCGGTGATGAAACTAATGGGTTTTTGAGTATGGGGAATCATTATAATCCTGAAAATAAAAAACATGGTAGCCCTTTTAATAATGAGAGGCATATTGGAGATCTGGGAAATATTTATTCAAACAAATATGGAATATCTTATATATATATATTAGATGGTAAAATCTCTCTTGTTGGAGATTATTCTATTATAGGAAGATCGTTAGTTATTAGTGAGAAAAATGATGATTTAGGCAAAGGATATAACTTTAAAAGCTTTATAGATGGAAACTCTGGAAACGGTGTCGCGTATGGAATTATCGGAATTGCATAATCTATTTTTTTTTAAATATATAAATTATAAAAATGACAAACACTTCCCTCCTTTTAAGCTACTTTTTCTTATTTTTGTACGGAAACTTGTTTGTCAAATCTGACTATAACTATTTAATGTATAAGATAAATGTTTTTAAAAACAATGAAAGTACTATCAAGTGTTATCATAATAATGATATTATTTTTTTATCAGATGATTGTGTAAGCTTTAACTCTACAACATATAATACAACTGTTTTTAACAATGATGATGTTAAAACTGAACTCGTTACATTATGTGATGTATCTAAAGAAGTACAAATATTCTCTCTCGACAATTCTTATAATGGTCTATTTTTAACTTTTTTATGCAATAACAACGATAGTTATTGGTTTGTTGATATTTTAGAAAATGGAATAGGTAACAATGTTCCTACATGTATCGATGGAGAAGTTAATAATAAAAAAGTTAAAAGTGAATTACAGGACTCTTTAGATAAGGAAAAAGTGTTGTTATCCACCTTTTGTAAAGTTTCTCATGGTGATTGTAGAATGTGGTTTAAAAAACATATATTTTATAAATAAAACAAAAAATATACTTATACTTTTAAAATATGAACATGTGCACATTTAAAAAATTTAGGTCGTTGTGTAAAAGCATTGCGTTAACGCCAAAATATTTAGAAAAAACAAAACTTATAAACAATTTCATAAGTTCACTTGATGTAGATAATAAGTACTTAGTAATAAAAATGTTATTACCAATGGTCAACAAACGGGTTTATTATTTAAACGATTTACAAATCATTAAAATTTTTAGTAAAATATTTAAACATGATTATAAGACCATGTTAGAAGATTTAGAAAATGGATATGTATCTAATACAATAAAAAAGTTTTTTAAAATCAGCAATACTAATATCGTTCCTATACAAAAAAGTATTTTATTACTTAACGATGTTGACATGTTTTTAAATCATTTAACTACATTAACAAAGGAAAAAGATAAACAGATGTTTTTAACAATGATATCCAGTATATGTACCGCCGACGATTTAAAATGTTTTATTTTATTTATCAAAAATGATTTGCAAATAAAAGCAGGTGTAAAATGTGTACTAGATGCATTAGATAAACATGCATATAATCATTTTAAAAATTCTATAGATTTAAAAAAAATAGTATTTGGTATATCTTTGAATAAGTTGAACGATGATAATATGGTACTTTTTACTCCTGTAAAACCTATGCTAGCAGATGTGTGTAAATCTAGCTATTATGCATTTAAAAAGTATAAAGATGGAATCTGTGCTGAAATAAAGTACGATGGCGAACGAATTCAAATTCATAAAGACAATAATTTATATAAATATTTTAGTAGAAATCTAAAACCAGTTTTATTTCATAAAATAGAGGGATTTGATGAGTTTCTAACCAGTGCGTTCCCTTCGGCTAATAACTTTATTTTAGATGCTGAATTAATATTAATTGATTTGAATACTAACAAATTTGTACCGTTTGGTTCATTAGGTATAAATAAAAAAAATTTGTTTAAAAACTCTAGCACTTGTTTATTTGTCTTTGATTGTTTATTTTACAATAATGTAAGTTTAATAAGTTTACCATTTGTTAATAGGAGAAAAATTATTGTTGATAATATCAATGAAATAAAAAATAAGGTTATGGTGTCAGAAGCATTTTATGTGTATAAAGAAGATGATTTAAATAAAATAATGGATGACGTTATTAAAAAAAAATTAGAAGGGTTGATATTAAAAGGAATAAATATCGATTATGTACCTGGAAAAAGAGGTTGGTTAAAGATGAAGAAGGATTACTTAGATGATGGATCCATGGCAGATTCTGCAGACTTAGTAGTTTTAGGATCATATTACGGTAAAGGGTCTAAGGGTGGGGCCCCATCAATATTTTTAATGGGGTGCTATGATAAGGAAAGTAATAATTGGAAAACGGTAACTAAATGCTCTGGCCACGACGATGACACATTAAAAAAAGTACAAAAAGAAATAGAGTTTTTAAAAATTAGTAAAGATACAAAAAAAATCCCAAAATGGTTAATAGTAGATAAAATATATTATCCAGATTTTGTAGTTTGTGATCCAAAAAAATCACAGGTGTGGGAAATATCGGGATCTAGTTTTACAAAATCGATACATCACACAGCAGATGGGATTTCGATAAGATTTCCTAGATTTAAAAAAATTAGATATGATAAAAATTGGTTATCGGCTACAAGTTTGAACGAACTTAAGAATTTATACAAAGAATCGTAAAATGTGTTGTTTTTAAAAAAAGAAAAAAAAAAGGCATTATTTTTTCTTTTTTTTTCTTTTTTTTTAAAAATATAAATTTAATACAAAGAAATAAATTGCTCCAAAGAAAGCAATACACACAATCTATCTGTTGTTTTTGATAAGGCATTGTAATATGGGAAAAGTTTTACTGAATTCATTATTCACTATGTTGATCATATTAATTTACAACACATATTGTGAGAAATTAGTTTGTTATAGAAAACTCGGATTATACAATTTTTACAATAAGGATATGAGATCACATTCATCGTTTGAATTGAATACCAAACGTCAAGACTTTGATGTTTTGAGGAAAGCAAATGCTATGGCACTTGAACAAAAAATTAATTGGACAATGTTAAAAGAAGAAGTAGATGATCTTTTTAAAAAAAATTGTACTGATTCTGATAATACAATTTATAACGGCATATTGTCTGAATCCATAACTTTTGAAATAAATCATGGATTTTTTAATGTGGATACTGAAGTCAGTATATCAAAATCATTATTATATATGGATTTAGGAGTAGAAAATTATACTGATGTATTAGAAAGTTACAACATTACAATATCAAACATGACAACGATGGCCACGCCGACGCCTGCTACAGTAATGACAACACCGTTATATTTAGAAAATAAAAATGACACCAATGATATTAAAGTAAAGCTTTACATGGTTAATTCAAGTATTATTCTAACATTTGAAAATATAACAATTGTTATAAATAATACATGTATTGGTACATCCATAAACAGTATTTATGCAAAAATAACAAGTGATTTTATAACTGTTAATGTCACAACAGATCCGTTGTCTACGACACCTCCATTTTTAACTAAGTCTATGTTTGATAATTGCACCTTAACACTTCCTGTTACTATTTCTCAAGAAGGATATTATGCAAAAGAATATAATGAATATACTGGTACGACTGAATCATTCACATATAGTTATTATTCAGAAGATAATATGACCGAATCATTTACTACTGCATTTAGTTATTATTCTGATGATACCACCACGGCTGAACCATTCACTACATCTAATTATTTAAATGATACCACGTCTGAACCATCATTTACTACATCGAGATACTATTTAAACAATGGTACATCTAATTATAAGAATATTTCATATGTATACAATAACAAAACAGGTATCGTATTTGTAAAACAACAAAATAACATTTTTAAAAATATAACCATACAAACAGAGTTTAAAAATTTATGTAACGAATCAAGTTTAGAAACAAAGGTATATGCTGTAGGGGTTCCGGAAAAGTTTAATACGATTTTAAAGAATATTAGTGTCGAAAAATCTAATGACAGTGATTCATATTTTATTTGTAAAATGATTGACGATGGAGGGAATTGCGGAATCGATATCTTCATGGATGCCGCAACTAATAATATTAATGATGAACTGGATAGTTCTAAAAAAGGAAGTAGTACCAGACACGCTAGATCTGTTGATCATATGGAGTTAGATCCATTCTGTTTGCATATGTATCACGGCATTGATGAAACCATAGATTGTTCACTAAAGAAAAACAATAAAGATACGAGTGGTCGCTCAAACAGAATCAGGAGATCTCCTCCTGATAAAGGTAAAAAACCACAAGTGCCCGCAAAAGGAAATTTACAACTAATGAGCGCCGAGGAAATGGGCGCTCGACCAAAAATAAGAAAACAAACGCAAGATATACAAATTGGTGCTAGAGGGGTCGATGGCCCAGTTTCTGGATCAGAAGAAATATATTCTCAAGTTAGGAAAGAACTATCTCAGAAGTTAAAATCTTTAGTTTTGGACGATAATGGAAAATTGACAACGACAAGATTGCCTAAATCGACAAAAGCTTTAATTGGTGGGTTGGTAGATTCTAAGATAGAGACATCTAGTGCTGCGTCTGATATAACAAGACAAATAGTCCATCAACAATCAGGAGATATTTATTCGTTGCCTGTAAAGAATAAAGTATTCGGTGTAGAATCAAATAGAAAGATCACATCTCCAATTATAGACGTCGATACATCATCTAATATATACGCAAACGTTTTACAAAAAAGAGATTTTAAAGTTACGTCTCCAAAAAATGTTGATGTATATGAAAAAACTAGAACTGATTCTTTTTCAGACGATTCGTATTTTTTGAAAAGATCATTTTCTTCTAGTTCCGACGATACTTACTTTTTAAAAGAAACAGATGATAGCGATAGTGACATTTTTAGGAGATCTTCAAAAAAGGATCAAACTTATTCACTAGCAGGAAAACCATTAAAAAAACAATCACTTAGAAGGTATTCATCTAGTGATTATGAAACAATAGGAGAAAATATATATGAATCTATTCGTGAACCAGAATATGCACTGCTTTCAAAACCAAGAGTTTTAAATCCACGAAGTCATATACCCCTACCATCAGTACCTAAGGATGACATACCATTCACTCAACAAAAAAGAAAGGTGATTGACATGATTTGTGACTCAAGGTCTGCATCCTCTATATGTAATGCAAGAGGGTTGGATTCGGCAAATTATAGAGGTGATGGTAACATATATGAAACAACTGACGACGATTTTGTGAAACGTGAAAATTCTTTATATGCAAGATCAAAACTAGAACCAGAATTAAAGGATAATCCATTGTATGAATCATCGTCCGATAGCGGCATTGTATCAAATCCATATAACAATCCTAAATTGTCGAGAAGAAATGCTATTAAAAAGAAAGTATTAAACGATGGTTATGAGGAATTCGTTATACGGACCGACGAAGAGCCTAATGAAAAACCTAATATGGCAGCAAATACGTATAATAACAATGATAAAGCTAATAATAAAGATAAAAATAAAGGATTTTCTTACTTAAATAATGATATACAAAAAGATGAAAAAAATGTTAATAAAATTAAAAAATCAAAAAAAGGAAGAACTAAACGTTTAACTGAATTATCCACTGATAATAAAATGAATAATATGATAAAAACAATTGCTATTTCCAGTTATTTATCATCAACGAATAGTAGGATATCGTCCATAATGGCACAGGCTAATCACCAACCAAAAGAATTAACAATTGTTAACATTGTAACGTCCGTATTATCTCAAATCGGTGGAACTTTAGCAATGGCTGGCTCTGGGTCTCCCAAAGCTGCAGCTGCAGGTTTAGTTATTCAAGGAATATCTGGATTAATAGATGCAGCGACATCAATTTATTTTCTTCTTTCTGGTCAAGAACAACCTAAAGATCCAGCTATTGAAAAGTTTTCCAACTACGCATCTTACGTTTCAAGGACAGATGCAGGAGCAAGGGTTTGTATGATGCCAGATTCAGATATTACAATAACGTTAGCATATAGACATAGTAACATGAATGTAGAAGCTGAAAAAACTAGAGGTGAGTACACAGATATAATTCCAAGTACCGTATACTACTTAAAAAACAGTCAAATAAGTTATACGGTGAAAGTAACATTGGTATGTCCAATCGGTCAGTTGAGGCTTTTGGAAGCAGATATAAATACATATGCCACACTTACTCGGGAAGATAAAGATGGTGTAAAATTCTATCATGTGTTTGGGATTTTAGAACTTCTATCATATCATCCTAATGTTACATTTACTTGTGGAAATGAACCAGGTGTAATCTTCATCCCGTTTGAGCAAAAATTAAGCGACATGCAATTATTAAGGATATCAACTCCTGGCGAACCTAGAGAAGCCGAAGCAATGAGTTCTGATGTTTGTGATATTTATCCTCTTAAGAAGTTTTATGTATTAGCTGGAAACTGCCCTTTTGATATGAGCAGAAAATCAGTAGCGTATGTTACATGTAGTACGTTACTAAGAATGTCAACGTATGAACATGAAAAACAGAGGTGGATTTTAATGAATCCGTTTTCAAACGGTAATGAGGACAATATTCAATTGTTTACGTTTAAAAAATATGACTTTAGTGCAGAAAAAGATAAAATAAAACTAAATAGTATTTCACATAGTGATACTATTTGTAGCCAATCTGATACTAGTACATGTTTTTGGGCAGATGCTATGATTTTAGAAGATGTAACATCTTGCAATTCTAGGATAAGAAAATTGTCAGTTGAGATGTCTACAGTATCAGAAAAAGGATATAACAATTTTGTACTTACGTGTCCATATGGGTCTACACCGTTTTATATAAGTAATGGTTCAATAATAAGTATTCCAATGAACACAAGAAGGACGTCGGTTAGATTTGCTTCTAAACATAACAATGTTGCACTAATATCATGTATACATAATTCAAACCCTGCATATAAATCGGATATCGTTGAAGTTATGTTTAAACAATCAAATATGAGTGATATGTATTTGGATTTCAAATATTTTAAGGATAGAAAATATTTATTTGATTCATTTAGTGATATAATGCCTAAAAGATCGAAAACTTGTAAGAGAGCATCAGAAAATAGCAATTGTAAAAACTATTATTATATTAAACATGTTCCTGAAATAGAGTTTAAAGTGTATGTTGCAAAACTTCCAATGGTTAGATTAGGCACAAGTTATTCAGGTGTTTTGAATTATCAAACATTAGAAAAAGTTAATAAGTATTTTTCAACACCTATAAGTATTTCTGTAGATGCTAGTACTCTATCTGATGTATACAAAAACCAAGAACATTTTTGGAAATTTGCAATGGAAAAGAAAAGAACTTTTAGTTCTATTACGGCTACCATATTTGCGTGCTCTGTTGTAGCTGGGAAGGTAAACGTAAACATGGGAGTTAAAGGTTCACACGATTATTATGGAAGAAGCGGTAAATATATTTACATGGGATCAAAAGATTTTGCATCTAATGATAAAATATATTTCAAATTTATACCTGACAAAGCAGAGTACCGTTTAAAGGATACATATGGCGAATGTGAAATTTATTTAGATTTAAAAACTAGGAGAGTGAATGTAAACTGTCCGGAATTGACTATACCACAACATCCATTTGATTCTCCAGATGTTAATAGTTTATGTGTGTTAGTTGCCACATCAAGAGATCATTGTGCTATATCTGAGGAAAATTGGAGAAGTTATGATAGAACTCATGGTGTTGGGTATAGCCACGAATACGTAGATGCGGAGTTTGATTCTTGTAGGAAGAGCCATGGTCCAACATATCCAGTTGATAACTTTTGCTTTTATTGGTGTGCTGGGATATATTGGCCACCTGACTATGATCCATGTGCTTCATCAATGGTGTTAGGTTATTCTCCGATTTTTCCAGAAAATAGAATTGTGCACCCTCCATATATAAAGGAATTTGGTTATGAACCAGGAAAAAATGAATACGTGAAAAGAGAGTTATATAACAAATTACAAAATCTTTATGAAAAATATAATATGTTGGTTTTATATTCAATGAACCCGGTTGTTGAAATGTCAAACGGATTAGCAAAATCTATGACGCCAGAAGGCCGTGAAATTTTTAGGTTAATAGCAAATAGTAACGAAATGCAAAAAGCTAAAGAAGAAAATGATATGAAAGCTGAGAAAGTTAAAATGGAAATAGAAGAAACATTAAATAATATTTACGTTGATACATTATCATACTCCGAATCAACTTCCCTTTTGCGGTCTGCTATTTCCACAAGGTGTTGTGTTTTGGACGGTACTAGCGTATATAAGTATTTTGATTTAGAATATTATCTTTGTGGTAATTATTCTGACTACTTAATTAATATTAATAATGTAACATATGTAAAAATTAATGATAGTCTAATAGAAGAAGATATCTATTTGGTAAGAAATATTCCACAGGTAACATGTTTTCATATAACGTTGGTGGCTGTAAATAACGAAGAACAACAGAAAAAGTTTGAAACAGAAATTGTGACAATGGCATTTGAGGATGTTTTAACTGAAATATTTGACGAGTATGATGATAGAATGGTTAGTTATTTTGACAAATATATAAGCGATGATAATAATAATAAAAATAGCAAATTTACCACCAATAACACAATTGCTATCATATTATCATTTGTTTTTACGGCAATAATTATTCTAATTTCTACTAAATTAATAATGCGCTCTAGAAAAGGCAAATATACCATACATAATAATTTTGTTATCTTTAAAAATAATAATAGCAGTAGTGAAGATGATAATACAGTTTCATTATCTGATTTAGATTTAGATAATAATAGTGAGTTTTTATATTTTTGAAAAAGTAACTTGTAAGTAATGAGGGTGTAAGGGGTTATAATTAAGTGAAAATAACTACAATCAATCAAAATGTTACTTTTAACTTCTTTTGTTTTTTCATTGTTGTTTTCGCATTCGTTAGGAAAAAGAAATTTGTCATATTTTGATACTGATGAATATAAAAATAGTATCAAAGATGTGTATGATTTTAGTAAAAGATTATTGGACGACGATGTGTTGTTTGCGCTAAATGATAACTGTACATTTAGAGATGAAAGAGGCAAACTCGCTACTCTTAAAGAACCACTTTCATTAAAATGTCCACTTTTAAACGAATATGGGTTAAAATGGCCGTATATGGAAAAGGATGATTATGAAATAAAATGGGAAGTTGTGCGTAATTACACTAAGAAAAGTATTAATAATAATACTAACAATTACTTAATTGAAGATGGAAAATTACTAATTTTAAAAACAAATCTTAATACGTTAAACAGTAAATATCTATGTACTATTACTAGAAAACGAGACAATGATTGCGATCAATCCATTGTGCAGCTCTCTAGATATAGAAATAAAAATTGTTATAAGTTAAACGGTTTAAAAGGCAGGAATATCGTGATTGAGTGTGGGGTTCGTCACGTAGAATATGATACTGTTGAGTGGTATAAACAAAATAAAACATCAATAATAAAACTTAATAACGATAGTAAAAGAAGAATTATTCTTCGTAATATCACTAAGGGCGATTCTGGAAAATATTATTGTAAAGGGCATTATAGCAAACTAAATATTTCATATACAATTAGTAGGTGTACGTATCTATCAGTGTTTCCACTAAGTCATTACGAATATGAATTAGTTTATATGCCAACTATTATTAACGTAACAATAGGAGAACCAATGACTGTAAACTGTAGCGTTCGTACAAAGGCAAACGCATATGAATATATTGGCGCTATGTGGTTAGATGATAAAAAATTATATGTAGGTATGGAAGAAAATTTATACCAAGACGGAAGTCGTAAAATTGAAGGAGACGATATTATTAAAACTTCACATTTGGTATTTGTTAATGTTATGGAAAAAGATATTGGAAGAACATTTACATGTGAAGTAGGATCATATTTTAAAGGAGAATATAGAACGGTTACTTTAAACTTAAAAAGTTAAAAAAAAATAAAATTTTAAGTTAAGTTATAATTAAAGAGCCAGACAGTATGGACAATAACATTGTTGACGTTGATGAATATCGTATGTGTTTTATATACGATAAGGTTGATTATATAAATATAGACGATCCCATTAAAAATATAATAGAAGAGTACTTTTTATGGAGGGGGTTAGTTGGTAGAATAGGAAGAAAACCATCAAAGATAGGAAAATTATTTGTAGATTTTATTAACTTAGATTTGACCGCAAAAAAAATGTTAGGAGACTTGGATTTATTGTTTACTGATATTTTAAAGTTAGATGATATTAATGGTGCAAACGAAAGACTTTCTAACTTTGTGAAGTTTGTCAATATTAATTTTAATAATAAACCTTTAGTGTTATTAGGATTGCTGGGGGCAGTTTCTGAATTTTGGGGAAAAAAGAAGATTAGTGTTAATGGTATAATGTCAGTGTTCTTAAGCAATATATCAAATGATACATTGCTTGAGATATGTAATCACATATAGTAGTTTTTTTATGTATTTTTATGTTAAAAAATAAAAATGAAAATGTACTTTTGATTAATATTTAAATGGATATTATACTTGGTAATACATTTTCTAACAATGATTGGGATATTTCAGAATTCTTTAAAAATGTATTTGAAAATAATGTATTCGAAAATCCAAAAAATATATGCTCTGTTTTTGATAGTATAATTATTGGAAATAAGACAAAAAGTAAAATTATTGTGGCAGATACACCCAAAATAGATTTATCTATCTCTAACACATACAAAGAAAAAAAAAAGGTAGATTTAATTAGAGTATCTAGATTTTGTAAAGCTGTGGCTCTTAAAAGTAAAAAAGACTATGTTTACATTCCCGAAACAAAAATTTCATTTGCCATACTTACTGTTTCTACAATAGACGATGATTTAAAACATGAACTAAGTTGTTGCTCGTTGCAAAACAAACAATATGATTTGTCGATGAATATGAATTCGTTTGCTATCGTTGCTAAATGTAATGGTCTATCAATAAAAGGTACTAATATGTTAATAGTCATAACATTTTTTGAAGAGAAAAACTATCCACATATTCCATTGATACGTACTATATCTAGTAATGATGTGTTTATATCTAGACATAGTAGGCTGCATAAGGAAATACCTAATAAAGATTGGTTTAAATTTTACGTAGAGTTTAATCATAATTATTGTACGGCATTGACGGTTATAATAGATGGAAGCATCTTATATTCTAGATCAGATTATAAAACTCATTGTATAATTAGTAAGTATCAATCAAAAAAAGACGAAATCAACGATGATTGTTGCTGTTGTTATAACACACCATCTGTTTATATACTTAATAAAAAAGAAATTATTGAATACGTGTCTTGCAATACAATAAGAGGAGGAATTCATATTTCATTAAAAGGTGTCGGGGATTTTAGTTCCAGTTATATAGGGAAATGCCCTAATGTTGAACATTTAAAAGTTGTTATAGGTTCAACATATGACATGTTAACAAAACAAGATAGCATATCTGGTAAAAAAATGTATTGTTCTTATATTTATGGAATTGCCCATAGATAGAAAAAAATAAAAATAAGTTCGGTATAAAAATGAATAAAATTAATAATATATTTAAGCTTATATGGATAAGTAATTATATTAATGTAATTTTATCAGCTACGTGTATCTCAAAAATATATAGCAGTTTTAAAGAAAACACTGTAAAGATTAGTTGTAACAAAACAAGCAAATTTAATTCTATTATTATAACATGGAAAAAGAATAATGAAACCATTGCTACGTATGGCCCACATGGTTCATACGTAGTAGATGATTATAAAAACAAAATAGAGTATATATCTAAATCATTTAATTATTCAACTATTATAATAAAAAATGCTACAATAAAAGATAATTCCTGTTATACATGCATATTTAATATTTTATTAAGTGAAAATGATAAGGGAACTTTATGCTTAAACACAACAAATGATGAATATATAAACAATTTAATTCCAATAAAAATAAATGAAAATAATAATCACATAAAAAATAATATTATTGTTGATGATTACGGTACTACAGAATCATTGTTTTCTTTATATGGTACGTTATGTCTTTGTTTGTGGATTTATATGTACTACGTTGACACTGTACGATTTTTCTTATAAAAGTGATTTTTTATATAATAAAATAAATTGTTAATAATATGCCAAAACGTAACATAAACGTTTTTGAGGAAGGTGATGTGTTAGTAGATTCTTTAAAGAAAGAATGGCGATTGGGGAAAATAATTGGTCAAGGAGGGTTCGGTTTCATATTTTTAGCATATTCACAAAATAATGAAGAATATGTGGTAAAAATTGAACCTAAGAGCAACGGACCATTATTTGTAGAACAAGTGTTTTATCAACGGATAGGGAAACGAGACATGATAACGTTATGGTCAAAAAATAATCATATAGATCATTTAGGAATTCCAGTATTTTATGGTTTTGGATTTCATAAAAAAAATGGAATAGATTATAGGTTTATAATTATTAATAGATTAGGTTGTGATTTAAATAAAATAATACAGTATAATAACAATAAACTTCCTGAAAGATCTGTGTTTTTAATAGCGTCTAAAATAATAATGATATTAAAATACCTACATGAAAATGGCTATACGCATAGTGATATTAAAGCATCTAATATAGCAATCGATATCAATAATAAAAATAAAATTTATTTATTGGATTATGGATTATCTTATAGATTCATGATAAACGGCAACCATGTGGAGTATAAGCGAGATCCCAAAAAGATGCATAATGGAACAATAGAATACACAAGTATAGATATGCATAAAGGTGTATCACCGTCTAGGAGAGGAGATTTGGAAATTTTAGGATATTGTATAATAAAATGGTTAGGCGGTAAATTGCCATGGGAAAATGATTTAAAAAATTGTAAATATGTAATGGAGTCAAAGATTAAATACATGAACGATATTGTAAATTTAATGACTGACTCGCTAGGACCTAATTATCCTGAAAAAATTTTAAAGTATTTTAATTATATAAAAACATTACAATACGATTCTATTCCAGATTATGAAAAAATAATGTCATTTTTTCTTCTTTAAAAAACATAAAAAAAAATTTTTTTTATTGACATGGATTCTGATATTCGCAACGGTTTTATTTTGCTAAATTATGAAAAAGTCAAAGTTATTATTGTGTTAGCAAATAATTATGTTAACATAACAAAACTTTGTAAACCTATGGGTAAATCATTCCACAATTGGTGGAATTTAAAGAGTAGTTATCAAATTATATGCAACATAGCAAAAGAAGAAGATATAGAAGTTGAAGATCTTTCATTTAATGTTTGGAAAGGACAAGAAACTAAAGATGTTCATGGAAAATATATTCATCCTAAGTTGTTGGTATATATTTTAAACTGGATTTCAGAAGAGTATTGTGCCAAAATTCTATCAATAATTAATGACTTTAATTCAAAAATATTAAATAATATTACGACAAATACCAAAATTAACAAAATTTATAAGCGTCTACCGAAAGAAGAGTCTATGTTTAATGCAATATTTAATAAAGAAAATAGCAAATCTAAAAATTACAAAAAGTTTCTAATAAACATTCCAACAATATTATCAAAATACGAAAAATATTTTTTGGAATCTGAAGAAAAAGAGTGCTCAGTTTGTTTAGAAAATGTATATGATAAAGAATATGATAGCATGTACTTTGGAATATTACCTAATTGTGATCATGTCTTTTGTATAGAATGTATTAATATTTGGAAAAAGGAAAACAGTACATGTCCAGTATGTAGGGATGAATTCTTATTTGTTATAAAAAGTAGATTTTTTCCATAAAAAATTTATTAATTGCAAACATGAGGAAAATATTTATTATTATAAATCATGAAAAATATACATATGTTATTGATTTTATTATGTAATAAAGTATATTCGTTATGTGATTTAAATAAATGTTGTTATCCTCCATCGATAAAAAATGGATACATATATAATAAAAAAACTGAATATAATATTGGATCAAATGTAACATTTTTTTGTGGAAATAACACGCGAGGAGTTAGTTATACTTTAGTAGGAGAAAAAAATATTATTTGTGAAAAAGATGGTAAATGGAATAAAGAATTCCCCGTTTGTAAAATTATAAGATGTCGATTCCCAGCTTTGCAAAATGGATTTGTAAATGGAATACCTGATAGTAGAAAATTTTATTATGAATCTGAAGTAAGTTTTTCATGTAAACCGGGTTTTGTTTTAATAGGAACAAAATATTCAGTTTGTGGTATAAATTCATCATGGATACCTAAAGTACCCATTTGTTCAAGAGACAATATTACATATAATAAAATTTATATCAATAAAGTAAATATAGATGATAACTTTTTTAACCAAATAAATAACAGTAATACTTATTACTTTGATAAAATATTACAAACAAATAATGTTAACAGATTTACTTTAATTTTTTTTGTTGTTGTATCAATTAAAATATTATTTGGTTTTGTATTTATATTCTTTTCGTGTAATAAAAAACCTTTGGATAGTATAAAATATTATAAATAAAATGGGAAATAAAAATGATGATATTAAACATATTATTAAAGAATATATTAGATGGAGAGGAAACGGTGAAAATATATGCGAAGAACGTGAACTAAATATTTTTAAGAAACTTAAAAAAATGGACTCGTTTGTAAAAAAAAATATTAAAGAAAGTAATAATGATGAATATGATATAATCAAATCTATGAAGTTATCGTTGGATGATGGTCCAAGGATGGATAAGCTTCCAGAATATTTGTTGGATGGGTTGAATGCAGAAGAAATACTAGGTCTATGTGGTATAATATCTGAAAAGGTTGTTAGTGCAACATATGAAGTAGATTGGGATCGTGTTTATGAATCGTTATTTAACATATTAGATGAAAAAGATTTAAACTACATTAAAAGTAAAATAATGTAAAACATAAAATAAATAACTTTTAAAAGTGATTATATCATGGACGAAAGAAACTCATTTTGGAGTTTAGTAAAAAAAATAACTACAGAAAATTCACAAAATATAATAAACCAAAGCTATATTAATCCAATAAGATGTATAAAATCAGATGATATTTGTCAATCTTATACAAGTATCGTTATAAAAGAAAATGATCAAAATTACATTCATAAAGGTATTTTTAATAATAAAGAAGTTATCGTTAGAACTTTCAAAAAATCACATACAAGTCACAAAATACTAATGGATATTTCAAATAATGAAATAAAAAATTTAAGAAGGATAGACAGTAATAATATATTAAAAATTTACGCATTTTATATAGAAATGTGCGACGGCCTTCCAAGGCTTTCATTGATATTAGAATATTGTAAAAGAGGATATTTAAAAAATGTAATAAGAAAAGAAAGAGATTTAACTTTTAAAACAAAATTAGATATGGCAATAGATTGCAGTGTTGGTTTATATAACGTTTATAAATACACAAATAATAAACCATATGGATGTATTTCAAGCGTTAGTTTTTTAGTAGAGGAAAATTATAAAGTAAAGATTATATGTCATGGTTTAGAAAAAATACTAGCCAATCCACCTTTTAAAAATATAAACGCTATAGTTTATCAATCTCATAAAATGTTATCAAATGTGTTTAATGAACGCACTATGGATGATGATATGTATAGTTTTGGCGTTGTTTTGTGGGAAATATTTTCTGGAAAAATACCATTTAAAAATCTAACAACGAAAGAAATATATGATTTAATCATTAATAAAAATGGACAGTTAAAACTTCCTGAAACGCCGCATTTAATTAAATATACAGTAGAAAAATGTACTTCACATACCAGTAAGAGACCAAATATAAAAGAAATTTTGTACAATTTTTCATTGTATAAATTTTACAACTAAAAAATCGTCTGTTTTTTATTTTTTTAAAAAATCTTTTAATAAAAATGAAACTAATTTAATAAAAGAATTTAAAAATAATAACTCATAACTAAGAGGCGATATAAATATACAAACTATATATTTTTATTTTTTAATAAAACAAAAATATATAGTTTGAGTTTTAAAAATATGTATTCATTTTACGAATTACGTAAATACTGCGATGTATCAATATGTTTTAATGATGTGATTAAAATTAAAGGTCATAAAATAATTTTATCAAATTCATCTAAATACTTTGATACCATGTTTAGTAATAAATATTTTATAGAAAACCAAAAAAATGACATAAGTATTAATATATCAAATTTAGATAATCCAGAGGTATCAATAAATGAGATAATTAAATTTATGTATAACGGGAAATTAGATAAAACTCTTAAAGATTATGTAATCTTAAAAGATATGCTTAAAATAGCAGATTATCTTATTATAGATGATATTATCCCACTTTGTATAACATCTATAGTTAAAATTATAGATTTTAATAATTGTCTCGATGCGTGTATTTTTTCAGAATTTTATAACTTAAAAAAACTAAATAGGTTTACTTATAAATTTATACGAAAAAATATACGTAAAATATATTTAAAAAAAGATTTTATTTATTTATCATTTAACGTTATTGATCTTCTTTTACGAGATAAAAAAACTGTATTTTATAATGAAGATGATGTATTATGTATTATATTACAATGGCTTAATAATGAAGAAAATAATAAATATTTTCATGATGTTTTAAAAGTAATAAGATTTTCACTTTTGTCAGAAGAATGTGCCATCAAGCTTAAGATAAAATATAAATACTTATTTCCTGATAGTTTATTAAGTAAGTCATTATTTTCCTCAAAAATGTCACGCAGAGAATCAACGTTTGGATCGTTTATATATATATCCGACCCAAATTGGTATAGTAAAAAATATGATATTATGTACATAAGAACATTTAATTATATTACAAAAAGAGTTAAAACAATAGATAGTATACCGTTTGTTGAAAAGTTTCATTCTGTATTACATAATGACGTTATTTACTTTTTAAGATTTGAAGGTAGTAATAATAAAAATAAATTTGATAGGAATTTTAACAGTTATGATATTGTAACTAAATCATGGAACAGTTTTCCAAAAATAGACGATTGTGAAAATTTTTCATCCTGTGTATTAAATAATAAAATGTACCTAATTGGTGGTGAAATTAATGGTATTTCAACAAATAGGGTATTGTGGTGGGATTTTAAATCTAATTATTGGAACCAAACAACTCCTATGCGTTTTCCAAAATCTGAATCATGTGTGGTACCGGCTGAAAATTTTATATTTGTAATAGGTGGAAAAGATATGTACTCATTAGATGTAGTTGAAAGGTTTGATACAAAAACACAATCTTGGTCAACTTTAATGCATTTACCTATACGATTAAAAAGAAGTTCTGGTATATATCATAAAGGATTTATTTATATAGTAGGGGGTATCAGTTACGCTAGTGCTGAGCTCGGAATAGGCTATGAAGGTTTTGTTAATAAAATATATAGATATGATATATCAAATAACTATTGGATTGAATTAAACCCATTAAGACATACAAAAATAAATGTTAATCTGGGTATATTAGATAATGACAATAAAATATATGCAATTGGAGGCGATAAAAATAATACAATAGAAGTGTATAATATATCAACAAATACATGGAGTATGTTTGGTAGATCGTTTTGTAATTTAATTAACTCAAAGCAATGTAATATATTTACAAAAAGTGTTTTTTTGTAAAAACATAAATAAAAGGGTTTAAGTTTATGAGCAAAATCATTAAAAATGATTTTGTTATACGATTACATTACGCTTACAAAATCTAGAAATATCAAAGTTAAAAATATAATAAAACTTATAAAAAATGATCAGCTAATTGATTTTAACTATAAATTATTTACGTTGTGTGAATATGTGAGCAAAAAACATATTCGTATAGATGTTTTAAAAATATTGTTTGAAATAGGTTGTAAAGAAAATTTACATAGGTTATCATATTATACTCTTTTAAGTTTCTTTAAAAACTATAAAATTAAGTATAATTTTAATCATGTTAAAAGTATTATAGAATTAATAACTAGTTATGGCGTATCGTTTAATGATGAACCAATCAAAAAAGATTCGTATCCCATTTTATTTTTAGTAAGTAATGAAAAATTTAATCATATTAGCCTTTATGAATATTTAGAAAATAATAATATAAATTTCAATATAGTAAGATCTGATGGTTATAACTTGTTGCATTTATACTTAGAATCCTGTAATAATATAAAACTAAGTGTATTAAAACTACTCATCAAAAATAATGTAAACGTTAACGGATTAACTAGATTTAGAAACCTAACACCGTTACATATATATTTGTGTAAAGGTTATTGCTTAAACTATAGTGTAATAAATTTTTTAATAGACACTGGATCTGAAATAAATTGCGGCAAGGAAACTTTATTATATTCATTCTTTACTACTTGTGATAATGAAAAAGTATTCAAATCACTAACTACTAAACTTATTAAAAAAGGAGCTGATATAAATCAAAAATCAGAAAGTGGATTAACGCCATTAATGGGATTTGTTGCATATTCAGATTTAGCTACTCCTAATAATATAAAATTCATACTAAGTTGTGGGGGAAACCAATTAGTTGTAAATCCTCAAAGTAATGAAACTTTGTTACATATTTACTTACGTAGGTATGATGTATCATTGTCAACAATATCAACGTTGTTAGAAAGTGGCATTAATATAAATTCTGTCAATTTTAAAAATTATACTCCTTTACACGTATATGTTGATAAAAATATAGAACATTTATCTTTAGATGTCGTGGATTATTTAATCTATAAAGGTGCTACTACCGAAAGAGTTAACAGGTATGATTGTTTTGCAAAAACTATATTAGAAGTATTTTTAGAAAAAAACAAGTTTTTAAACAATTCTTCAAGATATTTTATTAACTATATTTTAAAGTATTTTCCAATAAATGAAAAAGATCTATATGGATTTACCCCACTTTTATCTTCTGTATATGTAAATAATGTAAACTTTTTTAACTATTTTTTGAGATTAGGATCTAGTATAAATGTAATTTCAGAAATAGGAGAAACGTGTGTAAGTATACCAATTGAGAATCAGTATAAGCGTTTTTTAAAAATAGTTTTAGATAGTAAACCAAATATAAAAACAATAAAAGCTACTCTTAGATATCTTAGCGATAAGGAGTTTAATACAAGAACAAAGTTTAAATTGATGAAACATTGTATTAAATATTTATTCACTTTGGATCCTGACGAATATAAAAATCATAGGTTTATTTTTTCAAAGTTCAAAAGTGTCGTCAATGAATGTCAAAAAGATATTATAACTATGAAAAGTACATTTTTAAAGACTGTATCTGTTTACGATTTAATATTTAACAAAAATGGAACAATGCATAAAAGGTATTTAAATACCATGGAAGTAAGAAAATACTTAAAATCAAATATTTATGCTGAAAGAGTTAGATGTGTAATAAATAATTCAATAGAAAAAGATAAAAAAATTGATTCCATAATCAAAAAAGTTAATATATTATGTAAACATACTATTTGGGATATATTACCTGAAGAAATAAAAGACAATATCTTTAACTGTATGTCGTTGAGGGATATTAGAGTAATCTTTCATATGTTTGTTGTGTATAAGCCATAAGTCTTACTAATATTTTTTTTTGTTTTATGAATATAACCATAGTGTTAAGTGGTATCTTTTTATGTTTTAAATAATTAATTATCTTATAAAATATAATTTAAAAAAAAGTTTAAGATAAAAATATAATTATGAAAGATTGTCACGAAGATACGAAATTGGTAATTGTTGAAACAATACCATGCGATCTTGACTTATTGTATAATGTGTCAACATATGATTTTTGGATGAAACTTATATCAGAAACAAAGGAAAGTTTATATATCGCATCATTTTATTGGAGCTTATATGATGAAGATAGTAAAAAAGATGAAACTTCGAATAACGGAAAAATGGTATTAAAAGAACTAATTAAGTTAACATCTAGAGCATCTTTAAAAATAGTAGTTAATAAGTCTAATCAAACTTCTGAAGATTTATTAATATTATCTAGTTATGGGGCACAGGTTATCTACGTAGATATAAAAAATATTTTTGGTGGAGTTTTACATACAAAATTTTTAATTTCGGATGAGTTACATGCATATGTTGGAAGCGCTAATATGGATTGGCGGTCTCTTTCTCAAGTTAAAGAAATTGGTATAGGTATTTACAATTCTTCATGTTTGGTAATGGATTTAATAAAAATCTTTAATGAGTATTGTTATATTGGGTACACTAATGTACCTTGTTTTTGGACAAATGAATATCAACTATGTTATAATATCGATAATCCATTAAGTTTAATAAATAGTAATTATAAAATGTTTATAGCATCTTCGCCGCCATCGTTAAGTAAAAACAATGGTACCGACGATTTATATTCGTTATTATCTGCCATAAAAAACGCAGAAAAATTTATTTATATTTCTGTAATGAATTATATTCCAGTAATATATCAAAATAATAAAACACTATTCTGGCCTGATATAGATATAGAATTACGAAGAGTTGCTATAGATAAAAAAATAAATATAAAGCTATTGGTTAGTTTTTGGGATCATACACCTTTAATAATGAAAGGATTTTTAAAATCACTAAAAGATATAAACTATAAAAATATTAATATAGAGGTAAGATTTTTCATAATACCAAAAAATCAATTAAATATACCGTACACTAGAGTAAACCATACAAAATACATGGTTACTGATAAAGTTGCATATGTAGGTACATCAAATTGGTCCGGTAATTATTTTACCGACACCTGTGGTGTTTCATTAAACATAATTGATTTTAATGAATTATCAATAAGGTCAAAGTTAGAAAATATCTTTATAAGAGATTGGTATTCCGTATATTCATTTCCTATTTCATATGTCACACCAAAAGATAATAATGAAAAAGTACCTATAAAACAAGATAGGGATTTTTTAGATTATAAGCTATAAAAAGGTGTATTTTATTGAAAATACATGTTATTATTTTGATCATCTTCTCACCAAAAAAAATGTCACTGTTATATGAATACGTCTTACATTCTCCAACAGTAGATAAAGATGCAGTAGAATTATTAATTAGAACGGGATTTGATGTAAATGAAGAAAATTGTGATAATGAATCAATATTATTACTATATTTAAAAAGGAAAGATGTGAGTATTGATATACTGAGATTGCTACTAGAAAACGGTGCTGATGTTAACTACAGAAGCTATTATGATACTTCAATAACCAGTGTTATTAGGAACTATGAATTGGACAGTAATACTATAAAACAAATTATTATTCTACTATTAAAGTTTGGAGCGGATATAAACCTAAAACCGTTCAATGGTGTTTATACAATAATGCCTTTTATATATAATTTTCATATAAATAATTGTAATATGTTTAAGTTTTTGCTAAGTAAAGGAATATATGTTAAAAATGATATAATTGATTCTAGAGGATTTAATCTATTTCATATGTACTTTGAAAGTTTTTCAGTTAAAATAGATGTTATTAAAATTTTATTAAGTTTTAATGTTGGATTGTTTGAAAAATCATTTAAAGGATTAACGCCGATGAATATATATTTTGATCATTTCATAGACGTTATATCGTTGAAAGTTATCAAGTATCTAATTTCAAAGGGGGTAAATATTGAAACCAACAATAATGGTAGCAAATCTTTATTGGAAACTTTTCTACGATCTAACAAAATATTATCAAGAAAATGCTTTAAGGTTTTGAATTTTATTTTAAAATATGTAAAATTAAACAAAGTTGACGAAAAAGGACTTAATCCTATTCTGATATCTGCAAAGGCGGATAATTATGATGCGTTTAATCATTTACTGAAATTAGGAGATGATATATACAATGTTTCAAAAAAAGGAAATACAGTCATAACCTATGCAATAAAAAGAGAAAATATTGATATTCTCAATAGAGTATTAGCGATAGAACCCAAAAAATATCTTATAAAACAAACATTTGAATATTTTTCAAATTATGGATATGGTGGAATTAATGGATTGTTTTTAAGTGAAAGAAAATCACTAATGATGATATTATTAATGCAGTACTCTTTTAAAGTTTATCCGATATTTTACAAAAACTTTTACGAGGTTAGACTTTTATTTCCAAACATTATAGAAATGTATAACGAAGATATATTGTTAATGGAAAAAGAAAAGGTTGGAAAAAATATATCTGTATATGATTTAGTTTTTAATAGAGAAAAAGAAACTATCCCCGTAAAATTTTTAAAAAACGAAAATTTTTTAAAATTTACTTCGTCTACTATATACGGTGAACGTATAAAACAAATAATCAATAACACTTACAGATATGAAAACTGTTTGAATACATCAATTAATATACTGAACAAATATTGTAACAAAAAAAATTATTGGAATTATTTACCTACAGAAATAAAGATACACATATTAGAATATTTAGATTTTTCAGACTTTGATACTATTATTAATCCAAAAAGAAAATCAAAGAAGTACTTTTTATAATTATTGAAAAAATAAACATTGGTTTATGATTGGGGTCATAAGACACCATGGAATACATTAGCGATTATGAAGAACTATTAAAAGAGATTTATATCAATCAATCAAATTTAAAAATAAATGTGCTAAAAAAGATAATAAATGATATACCTACAACATATCATAAAGGTATTTATAACAATTTATTACTAACAGTTTTGGGCAACAAAAGTATTAGTCTTAGTGTACAAAAGTATAAAAAAATTTTGTCATTTTTAGTAGATAATGGTGCTGATTTAAACACAAAAATTAAATACAAATATAATGCTTTACATTACTACTTATATAGTAATTCAAATGTTACAGTTGATATAATAAAATTTTTAATAAAAAAAGGAGCTGATATAACAAAAAAGTGTAATGGTGACAATGTCTTACATACATATTTGTGTAATAAAAACATAGATTTTAAGGTTATTAAGTTTTTAGTTAATAAAAAGATAGACTTAGGCGATAGGAATTTAGATGACCATACTCCAGTTAATATATATATAAGGAATAAGAGAAACAATTGTGAGATCGATACATTGAAATTATTATTTCTTGTTGATTTTAATATATACAATAAGGAAGATATTTTCTTATCCGCATTAGATGTATTTTTAGATTATTTAAAATCATATACACGTAAATCCTTAGATATAGTTAATTATATATTAGAAAATATATCAATAAATTCTGTTGATTCTAATGGCTTTAATCCAATTTTATACGCTACAGTATCAGGACAAAAAGTATTTTTTGATTATTTTTTAAAATTAGGATGTAGTATAAATATAACAACATCATGTGGAGAAACTTGTGGATCCTTATCGTTGATGGATTGTGATTATTGTACTTTTAAAACTTTTTTAAGCAAAAAACCAAATTTACAAACAATCGAAAATACTTTAAGTTGCTTATCAGATTATTTAGAAGATATTTACTATTGTGATTTAAAGTTTAAAATGTTTAAAGAATTACTTTTAGAAGCTTTTATGTTAGATAGTGAATTTTACAATAGACACGAATCAATCAAATTATATTTTCCAAAAACGATTTCATTGTATAAAGAACCGATAGTGCAAATGTGTAGTGATAAAATAGGTGATAAATCTGTTTATGATATTATTTTTAAAAACAGTGATATTAGGTATTGCTATAATGATTATATTAACAAATATACTAACTTAAAATATTATGGAAATCTTATAAGGAAATGTATTTTAGCTTCTAAGATGAGGAAAAAGAATATAGTTAAGATTATAAAGACAATTAATATTGCACCTTACTGGAATACATTACCAACAGAGATAAAAATGTACATCATTAATTTTTTGAGTGATAATGAAATAAAGTTGTTGGCAATAAAATGAAAAAAAAAATTTTTTTTTAAATGGATATATTTACGCACCTATCATCTATAGCATCTCCCGATTCAAATGTGTTAATTTCTCCTGTTTCTATATCGTCAATACTTTCAATTTTACTTTTTGGGTCAAATGGTGATACAGCTATACAAATATCATCAGTATTAGAAAGTACGTATAGTAATTGCTGTTCGGACGATATAATCATTGCAAATAGAATTTATGGAGATTTGAAATTGCACTTAAAAACACAATTTATTGATAAATTTGGGAAAGAACTTATTTTGGTAAATTTTAATCATAATACCGAATTAATAAAAAATGATATTAATGAGTGGATAAAAAGATTAACTCATGACAAAATAAAAAACTTGATAGATGAAATAAGTGAGAATACAAAGCTGTTATTATTAAATGCTGTGTATTTTAAATTAAAGTGGAAAAGTCCTTTTGATAAACAACAAACAAAAATAGAAAAATTTTGGTATGATAATAGTAGATATGAAAATATTGAAATGATGAACGATGTTAATGTTTATCCTTTTATTGAATTAAAAGAACTAGGATTAAAAATTATTGAACTACCTTATGAAAACAATTATTCAATGATAATATTGTTACCAAAAGATATTAAAAAAATAGAAAAAAAATTGACTGGTAATAATTTAACTTTGTGGATCGACAAAATGAACCTATACGAAGTTAATGTAAAAATTCCAAAGTTTAAAATAGAAGAAACGTATGATTTAAAACTAAGCTTAATTAGTTTAGGTATTATTGACATATTTGATGGATCCGCAGACTTTTCGAATATGACAAAAAACAAAAACCTATCTGTTGATAATTTTTACCATAAAACTTATATAGAAATAGATGAAGAGGGCGCAGAATTATCCGCAGCATCATATTGTTGTGTAGCTGATTGTGGATGCAATGAAAAAGAATTTATAGCAAACAAACCTTTTATATTTTTCATAAAGGATAATATTAACGTATCTTTTCTTTTTATCGGAAAATTTAGTTTTCCCCATATTCGTTAAAAAAATTAAAAAAAAATTACTTACTTCTAAACGCAAGAATGGATACTAACACTCTTCCTTTTAGTAAGCACTATATTCATGATTTTACAGATTATGATAATTTTGAAAAAAAGAATAGTTTTTCGATGTCTTACCAACTGACAGGCATATTACAGTATTACTTTATTTGGAGATTTAATGGTGGTAAAGTTGATGAGACTACAGGGAAAATTTTTAAAGAGTTTTTTAAATATGATACATTAGCAAAATGGAGATTCTCAAAAAGAGATTTTGAACAGTGTAAGAATATGTTATTGGAAAATGATAAAAATATTTCATATGTGTTAGATGTTCCAAAACCAGAAAAAAATGATCCGTTGTTAAACTTAAAAGAATTAATTGGATTCTTGGCAATTTTATCAGAAGAAATTAAAAAAAATACAAAGTTTTTCGCATTGTTTAATATAATGAATTCGTTATTTACGCATACAAAAATAGATATTGTAAATTGCTTAAAAACAGAATTACAATTTTTATATCCTTAGTTTGTTTGCAAAATTGAAAGAAAAAATTTTTTTTTATGGAAAATGTACGCCTTTTACGAACAACAGAGGTTTTGTGACGTTACATTGTTGTTCAACAATGGAAAAAAATCTATTAAAACTCATAAAATAATACTATCATCTTGTTCAGATTATTTTAATGTTATGTTTAACAATAATTTCATTGAAAATATTATAGATGAAATTAATATTTATATAGATATAGAAAATGATAAATCGTTAGATGAAGTTATAAAGTTTATGTATTTAAAAAAGTTTAATTATGGCATAAATAATTATAAAATTTTAAAAGAAATGATATTTTTAGCAAATTACTTTATTATGGATAATTTAGTAAATTTATGTATTGCGTCTATCATAAAATTTGTCGATGTAGATAATTGTATAGAGATATACAAATTTTCACAATATTATGGTTATAAAAAATTGTCATCATATACTTATGATTATATAAGATGTTATATAGAATCAATATATAATCAAAATGAATTTACAACATTAACTATTAATGATATTATGAAATTACTATCTGATAGGAAAACTATCGTTGCATCGGAAAATAGTGTTGTTGAAATACTAGTTAGTTGGTTAACTTCACAAAATAGTATAAGTTGTTTTGAAATTTGTAAAATACTCAAACTTATTAAAATACCATTTGTTACAGAAGAATATTTTAAAAAACTACAATATCATCCCAAAATATTATATAATGAAAAATGTAGAAAAATATTAAGAGTAAAAAATAATAATATAACACCAAGAGCTTCGACATTGGGTTCTATTATTTATATATCTAATCCCAACGATCATTCCGGCGGAAATCCAATATTTATGGCAATATATACGTATAACTTTGTCACCAACGAAATAAAAGCTATAGATAATATACAGTATGCAAATAACTTCTGTTCGATATTTTATAATAATATATTTTATTTTATAAATTTTTCATACAGAGTAAGGGATTCGTTAGTTCATGACGATTTCCAAAGTTATAATATTATCACTAAGGAATGGGGAAAAATTCCCAAGATAAGTGATAGAAAGGACTTTTCTATAATAATTTTTAATGAAAAATTATACGCAATTGGAGGAATAAAAAATGGATCCGTTGTATCAGATGTTAGTTTTTGGGATTTAAAATCATCAAAATGGGAAGATGCCCCACCTCTTATATTTCCAAAATCTAATATGTCTTTAGCAAATAATAATGAATACATTTTTGCAATTGGAGGTAAAAATCATGAACTACTTAATAATGTTGAAAGGTTCGATATAAATGCTCTTAAGTGGGATAACGTAGCACCGCTTCCTATACCTTTATATAATTCCTCCGCCATAGCATATAAAAAATACATATATGTTATTGGGGGGAAAACATATATAGATTTACCAGAACGTTACAATATCGATCCAGTAGATGGCTCTAGTAAAAATTTGTTCATGTATAATATCGAATATAATGTGTGGAATGAACTTAATATGATGATATTTACAAAGGTTTTACCTAGTTTGGCGATCATTAATAATAAAATTTACGTAGTTGGTGGAGATAAAAATAACCTTATAGAAGTGTATGATATAAAAAAAAATTATTGGTATATTTTTAAAAAATCATTTCCTAAATCACTTTCTAAGCAAGAAAATATTTTCACTAATAAAGTTTTTTTATAAGAAAAATCAAAAAAAAATAAAACTTAAAAATTACTTATGATTAATTAAATAAGTATATTTACAAAATGGATATTGATAATATATATAATTATATAGCTTTTAGGGGTAATCCTAAATTAAGTATAATTAAATCATGTGATCCAAACAAAATAGTTAATGAGTATTCACTGTTTCAAAAATACTTACAACGAGATTCGCCATCGATCGATATTGTAAAATTATTAATTAATTTAGGTGGTAATGTTAATGGATTAGAAAATGAATACTCAACACCTTTATGTACAATTTTATCAAATATAAAAAAATATAAACATATGTTAGATGTTGTTAAAATATTAATCGAAAACAATGCAGATGTTAACAAAAAAAATTCTGAAGGCGAGACACCTTTATATTGTTTACTATCAAATGGATATATAAAAAATAATAAAGAAATTTTGTTATATATGATACAAATGGGTGTCGAAACAACATTGCTATCTAAAGATGGATATACAATGCTTCAAGTATATGTTAAAACAAATCATCATATCATTGATATAGAAATTATAAAAATATTATTAGGAAGTGGTATTGATATAAATACAATAAGTAATAAAGAAAAGTATGATACATTAGATTGCTATTTCAAGTATAATATCGATAGGATAGATGCAAATATTTTGAAATTATTTGTTGATAATGGATTTATAATAAAAAAAGAGGATAAATCTCATAAAAAAAAACTCATGGAATATTTAAACTCGTTATTATACGAGAATAGAAGAGTTAAAAAAAATATACTCGATTTTATATTTACATATATTAACGTTAATCAAGTAGATGAATTAGGATTTAACCCACTATACTATTCAGTTTCTCATAATAATAGGACGATATTTGAATATATTATCCAATTGGGTGGTAATATTAATTGTATATCGGAGTTAGGCGACACTTTATTATTTAAAGCATTTGAGAATCGGAGTCTTTTCATATTTAATTCAATACTTGAAAAAAAACCAAACAAAAAAACAATTTCTTATACATATTATAAACTAAGAAAACATCTTTTAGATGTAGGCGATTTTATTAATCAAATAGAGTTTGATATTATTAAAAAGTTTATAGCGCACGTAATTTTGTATGTAAAAAATTTCAGCGTTCGAAATCGAACAAAAGCATTTATTTATTTTGATGATTTTATAGAAAAATGTACAAAATCTTCTAATTACATTCATAATACGTACATAAATAATGAAACAATTTTTCAACTTTGCTTTAATAAAAAATATATACCAATAAGCGTGATAAATAATAACGAAAAACTGTTAAAAAAACATACAAAATTATTTTACTATGGAAATATTTTAAAGAAAAACATAGAAAAGTCTAAAAAATATTATGAAAATATTTGTAAAGTATCGTGTTGTATTTCTAATTTATGTGATACATGTAGTTATTGGAATACAATACCGTTAGAAATAAAATTTAAAATAGTAAATAATTTATCACTTAATGACATAGAAATGTTTTTGAAAAATAATAAAAAATAGAAAAAAATATTTTTATTTTTTATATTTAAATACAAACAACAACTATGATTTCATATATTTTAAGCCCTTTACTAAGTGTTTTCTATTTTGTTATAGGCAAAATAACTAATCTTGTTACATTTTTATTTTTGAAGTTTTTTTGGGCAATGATAGGGTTAATGAATCCTTATAACATGATTACCTCATCGACAAAAAATCTGTTTTCGTTTAATATAAACCCCTTTAGGCAAAAGAAAAAAAGTTTCAGTTTTCTATCATATATAAATCCTTTTAAGAAAGAGGAAAAAAAGAAAGGTTTGTTTTCAAGTTTTTTTAGTTAATAATAGAAAAAAAAGTTCAGTAGTATCGAGGTGGTTTGTGTCGGTAATACGTCAAAACAACCAACCAATATGTCATCATTAACTTTGTTCATTTTCTTTTTGCAACTATTCATTTTTACTAGTAGTGTAAGCGGAATATCAATAAAGAGATGCACAGAAGAAGAAAATAACACATGGGAAATCGAAGTAGGATTATGTATCCAAACAGAGAATTTTAGAGCGATTAAAACTGGCTGTTATAAAATTCAAGGACCTGGAGGACTTCTAACAGAGGGAAATGGATTTAAAATTTTCGCACATGATGATTGTTCCAAAGAAGAAACACAAAACAACTTTATATTAGATAGTGTTAACGAAGCAGTTTATGCATTGGGTAAATATGTATATATGGAAATATCAACCAGTAATATAACAACTCTGTATTCGCTGCCACAGTGTGCTAAGAGAATATCATTGTCAATTTCTTGTGATCAAGTAACTACAGAAATGAAATCATACGTTGAGAGTGTAAGTTTTAAAGATTATGATTTAGAATTTGTTATAACAACAGATATTAGTTGTGTAAAACATGTAAGTTCAAGTGTTATAGTTAGAAATGAATGTGAAAAAAAATATATATCTACAGGAAAAAAGATTTTTGGGTTTAATAATAAAATAGATTGCTCAGCTGTAAAATTTAGCGAACACGTAAATTATTTAAAAACGTGCAGTGTTGGTAAATTTGATAGAAAAAAATATTATGAACATCAGCATAATTATATTAAAAAAATATTTCATCATAATGAACTATAGGATTGTTATACAAAAGACGATATAATATTTATTTTGTTCTTTTTCACATATATAAATTATGAATAAGAAACAGGAGTTTTCCGTTTTTTTTTAGTCTGGTGGATTTTTGCATTAATTCATAAAATAATCTTTAGTATATGGAGATGTGTAAGAGGGACATTAGTTCTTATACTAAAGATTTTAGGATTTGTGATATATGCAATAAAAGGATTTTGTCAACTACTCATTAATAGCTTAGCTAAAGTTTGTATGTTGTGTATTTTTTATATAGCTAGATTATGTAATTTAATTATATATAGTTTGTATTCATTATTAATGTTTCCAATGCAAAAGCTTATCTCCTTCATGTTTGGAAACCTAAATCCGTTCGATTCTGTCTTGGATAAGGATGAAAAAATCCAAGACAATATAAACACTAATCACAAACCTATAGAAAGTAAAGAAATAAATAATGATCTACCATTAACTGTATTAGATAAAAAAGATACAAGCGATATAAATATTAGAAATGATAATGGAGTTTTTGATTTTATTAAAATTCCTAATCCGTTTAAAAAACACTACGAATACTACTGTAATCAAAACACAATAAAGGAACCACCTAGAAAAGGATTAGTTGAAAGGATGATGAATATGGTTGAATAATTTTGAAAAATAAGCACAGGAAATAACAAGTTCGGGTTAAAAGAGACTTTTTTCCCGTTCATTTTACAAGATGTCTTCCGGCAACTATGTCTACCGAAACAACTTTTCAGATGATGATGATATCACAACCGCCATCTCTGATTATTTGTTTTGGTCATCACTGGCATTTTCCTCCAGGGAGGTTGCTGGAAAGGTGTTCTTAGTTTTTGAATCTTTCAAAAAGGATGCCTCACTTGTATTTGGAAATGATCTAACAGCTTTTGTCAAAAACATGTTTTTGGATTCTAAAATTGGATTTGAACAATCAAAAATTATGATTAATTCCATGTTAAAAAAAGAAAATTACATTAGGGAATCATGTGCAGTGATTGGCATTTTAGCAAGAGCAGCAGAATATTGGGGTGGTGAATCATCTCCAACTTGTTCTTCTGTGAAAGTGTTGGTATTGCTTCGAGACCTCGTTTCTGACAACGATATTTCGCTAGTGAAATCAGCACTAATAATTAGACTTAAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN diff --git a/workflows/wftest.yml b/workflows/wftest.yml index 6bb2104ee..08f7d0790 100644 --- a/workflows/wftest.yml +++ b/workflows/wftest.yml @@ -8,17 +8,13 @@ jobs: name: Setup cache uses: galaxyproject/iwc/.github/workflows/setup.yml@main with: - python-version-list: "[\"3.7\"]" galaxy-fork: galaxyproject - galaxy-branch: master test: name: Test workflow needs: setup uses: galaxyproject/iwc/.github/workflows/test_workflows.yml@main with: galaxy-head-sha: ${{ needs.setup.outputs.galaxy-head-sha }} - python-version-list: "[\"3.7\"]" galaxy-fork: galaxyproject - galaxy-branch: master repository-list: '.' check-outputs: true