From 7bbf4536dfa126e1e572896ef5bc3684cab46a05 Mon Sep 17 00:00:00 2001 From: Dani Welter Date: Mon, 13 Aug 2018 15:54:38 +0100 Subject: [PATCH] Added two new sequencing technologies required for HCA --- src/ontology/efo-edit.owl | 48 +++++++++++++++++++++++++++++++++++---- 1 file changed, 44 insertions(+), 4 deletions(-) diff --git a/src/ontology/efo-edit.owl b/src/ontology/efo-edit.owl index 27ee3351..1ec6dc4c 100644 --- a/src/ontology/efo-edit.owl +++ b/src/ontology/efo-edit.owl @@ -487195,7 +487195,7 @@ label: granulocyte colony-stimulating factor measurement - Single G2/M nucleus sequencing of cells in S phase (Nuc-seq) + Single G2/M nucleus sequencing of cells in S phase (nuc-seq) Dani Welter PMID:25079324 nuc-seq @@ -491674,7 +491674,6 @@ label: granulocyte colony-stimulating factor measurementDani Welter 10X 10x - 10x_v2 10X sequencing @@ -503974,6 +503973,47 @@ It allows sampling of specific cells under direct microscopic visualization by a + + + + + + + + + + + + + + + + + + + + + Div-Seq is a single-nucleus RNA sequencing technique that improves upon sNuc-Seq by incorporating 5-ethynyl-2’-deoxyuridine (EdU) labeling to identify dividing cells during their different cell stages. EdU labeling also enables identification of different cell types in complex tissue samples and rare cell populationsduring FACS. Briefly, samples are labeled in vivo with EdU, dissected, and fixed before isolation into single nuclei. Individual nuclei are tagged fluorescently and sorted by FACS. From this step, the procedure follows the Nuc-Seq method: cDNA synthesis is performed using the Smart-seq2 protocol, while the cDNA library is prepared with Tn5 transposase–mediated tagmentation. + Dani Welter + PMID:27471252 + Div-Seq + + + + + + + + + 10x v2 is the second version of the 10x sequencing technology. + Dani Welter + 10x v2 + 10x_v2 + 10X v2 sequencing + + + + @@ -720186,8 +720226,8 @@ In EFO's scope, an obsolete class that holds an external-ontology namespace in_proximal_side_of uberon X proximal_side_of Y <=> if Y is subdivided into distal and proximal portions, X is part_of the proximal portion. - in_proximal_side_of in_proximal_side_of + in_proximal_side_of @@ -720197,9 +720237,9 @@ In EFO's scope, an obsolete class that holds an external-ontology namespace BSPO:0000125 - in_distal_side_of in_distal_side_of X distal_side_of Y <=> if Y is subdivided into distal and proximal portions, X is part_of the distal portion. + in_distal_side_of in_distal_side_of uberon