diff --git a/src/ontology/efo-edit.owl b/src/ontology/efo-edit.owl
index 4194558f..ada51357 100644
--- a/src/ontology/efo-edit.owl
+++ b/src/ontology/efo-edit.owl
@@ -16673,6 +16673,8 @@ Declaration(Class(efo:EFO_0022379))
 Declaration(Class(efo:EFO_0022380))
 Declaration(Class(efo:EFO_0022381))
 Declaration(Class(efo:EFO_0022382))
+Declaration(Class(efo:EFO_0022383))
+Declaration(Class(efo:EFO_0022384))
 Declaration(Class(efo:EFO_0022386))
 Declaration(Class(efo:EFO_0022388))
 Declaration(Class(efo:EFO_0022389))
@@ -200917,6 +200919,22 @@ AnnotationAssertion(oboInOwl:hasExactSynonym efo:EFO_0022382 "PASI")
 AnnotationAssertion(rdfs:label efo:EFO_0022382 "psoriasis area and severity index"@en)
 SubClassOf(efo:EFO_0022382 efo:EFO_0001444)
 
+# Class: efo:EFO_0022383 (protein extraction protocol)
+
+AnnotationAssertion(obo:IAO_0000115 efo:EFO_0022383 "A protocol that describes how to isolate protein from a tissue, cell culture or other biological samples.")
+AnnotationAssertion(dc:creator efo:EFO_0022383 "Zoe May Pendlington")
+AnnotationAssertion(dc:date efo:EFO_0022383 "2023-10-13T09:00:22Z"^^xsd:dateTime)
+AnnotationAssertion(rdfs:label efo:EFO_0022383 "protein extraction protocol"@en)
+SubClassOf(efo:EFO_0022383 efo:EFO_0000490)
+
+# Class: efo:EFO_0022384 (DNA extraction protocol)
+
+AnnotationAssertion(obo:IAO_0000115 efo:EFO_0022384 "Nucleic acid extraction protocol that describes how to remove and purify DNA from an input material. This requires physical and/or chemical methods in order to separate the DNA from cell membranes, proteins and other cellular components.")
+AnnotationAssertion(dc:creator efo:EFO_0022384 "Zoe May Pendlington")
+AnnotationAssertion(dc:date efo:EFO_0022384 "2023-10-13T09:01:13Z"^^xsd:dateTime)
+AnnotationAssertion(rdfs:label efo:EFO_0022384 "DNA extraction protocol"@en)
+SubClassOf(efo:EFO_0022384 efo:EFO_0002944)
+
 # Class: efo:EFO_0022386 (HEP-3B cell)
 
 AnnotationAssertion(obo:IAO_0000115 efo:EFO_0022386 "human hepatocellular carcinoma (HCC) cells (p53-null).")