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ERC000032

ERC000032: ENA Influenza virus reporting standard checklist

Minimum information about an Influenza virus sample. A checklist for reporting metadata of Influenza virus samples associated with genomic data. This minimum metadata standard supports submission of avian, human and mammalian surveillance data as well as serology and viruse isolate information (where available). The ENA Influenza sample checklist is based on standards in use at the Influenza Research Database.

Study

A Study is a container for a sequencing investigation that may comprise multiple experiments. The Study has an overall goal, but is otherwise minimally defined in the SRA. A Study is composed of a descriptor, zero or more experiments, and zero or more analyses. The submitter may decorate the Study with web links and properties.

Field name Cardinality Description Controlled vocabulary
alias mandatory Unique identificator for a study. this is used to link experiments to the study.
title mandatory Title of the study as would be used in a publication.
study_type mandatory The study_type presents a controlled vocabulary for expressing the overall purpose of the study. Whole Genome Sequencing, Metagenomics, Transcriptome Analysis, Resequencing, Epigenetics, Synthetic Genomics, Forensic or Paleo-genomics, Gene Regulation Study, Cancer Genomics, Population Genomics, RNASeq, Exome Sequencing, Pooled Clone Sequencing, Transcriptome Sequencing, Other
new_study_type optional Optional if 'study_type' is not 'other'. to propose a new term, select other and enter a new study type.
study_abstract optional Briefly describes the goals, purpose, and scope of the study. this need not be listed if it can be inherited from a referenced publication.

Experiment

An experiment object serves as a metadata record encapsulating essential details about a sequencing experiment, including the experimental design, sequencing type, and relevant parameters. This information enhances the interpretation and contextual understanding of nucleotide sequences submitted to the archive.

Field name Cardinality Description Controlled vocabulary
alias mandatory Unique identificator for each experiment. this is used to link runs to experiments.
title mandatory Short text that can be used to call out experiment records in searches or in displays. this element is technically optional but should be used for all new records.
study_alias mandatory Identifies the parent study. (from study metadata)
sample_alias mandatory (from sample metadata)
design_description mandatory Goal and setup of the individual library including library was constructed.
library_name optional The submitter's name for this library.
library_strategy mandatory Sequencing technique intended for this library. WGS, WGA, WXS, RNA-Seq, ssRNA-seq, snRNA-seq, miRNA-Seq, ncRNA-Seq, FL-cDNA, EST, Hi-C, ATAC-seq, WCS, RAD-Seq, CLONE, POOLCLONE, AMPLICON, CLONEEND, FINISHING, ChIP-Seq, MNase-Seq, DNase-Hypersensitivity, Bisulfite-Seq, CTS, MRE-Seq, MeDIP-Seq, MBD-Seq, Tn-Seq, VALIDATION, FAIRE-seq, SELEX, RIP-Seq, ChIA-PET, Synthetic-Long-Read, Targeted-Capture, Tethered Chromatin Conformation Capture, NOMe-Seq, ChM-Seq, GBS, Ribo-Seq, OTHER
library_source mandatory The library_source specifies the type of source material that is being sequenced. GENOMIC, GENOMIC SINGLE CELL, TRANSCRIPTOMIC, TRANSCRIPTOMIC SINGLE CELL, METAGENOMIC, METATRANSCRIPTOMIC, SYNTHETIC, VIRAL RNA, OTHER
library_selection mandatory Method used to enrich the target in the sequence library preparation RANDOM, PCR, RANDOM PCR, RT-PCR, HMPR, MF, repeat fractionation, size fractionation, MSLL, cDNA, cDNA_randomPriming, cDNA_oligo_dT, PolyA, Oligo-dT, Inverse rRNA, Inverse rRNA selection, ChIP, ChIP-Seq, MNase, DNase, Hybrid Selection, Reduced Representation, Restriction Digest, 5-methylcytidine antibody, MBD2 protein methyl-CpG binding domain, CAGE, RACE, MDA, padlock probes capture method, other, unspecified
library_layout mandatory Library_layout specifies whether to expect single, paired, or other configuration of reads. in the case of paired reads, information about the relative distance and orientation is specified.
insert_size optional Insert size for paired reads
library_construction_protocol optional Free form text describing the protocol by which the sequencing library was constructed.
platform mandatory The platform record selects which sequencing platform and platform-specific runtime parameters. this will be determined by the center. optional if 'instrument_model' is provided. LS454, ILLUMINA, HELICOS, ABI_SOLID, COMPLETE_GENOMICS, BGISEQ, OXFORD_NANOPORE, PACBIO_SMRT, ION_TORRENT, CAPILLARY, DNBSEQ, ELEMENT, ULTIMA, VELA_DIAGNOSTICS, GENAPSYS, GENEMIND, TAPESTRI
instrument_model mandatory Model of the sequencing instrument. 454 GS, 454 GS 20, 454 GS FLX, 454 GS FLX Titanium, 454 GS FLX+, 454 GS Junior, AB 310 Genetic Analyzer, AB 3130 Genetic Analyzer, AB 3130xL Genetic Analyzer, AB 3500 Genetic Analyzer, AB 3500xL Genetic Analyzer, AB 3730 Genetic Analyzer, AB 3730xL Genetic Analyzer, AB 5500 Genetic Analyzer, AB 5500xl Genetic Analyzer, AB 5500xl-W Genetic Analysis System, AB SOLiD 3 Plus System, AB SOLiD 4 System, AB SOLiD 4hq System, AB SOLiD PI System, AB SOLiD System, AB SOLiD System 2.0, AB SOLiD System 3.0, BGISEQ-50, BGISEQ-500, Complete Genomics, DNBSEQ-G400, DNBSEQ-G400 FAST, DNBSEQ-G50, DNBSEQ-T7, Element AVITI, FASTASeq 300, GENIUS, GS111, Genapsys Sequencer, GenoCare 1600, GenoLab M, GridION, Helicos HeliScope, HiSeq X Five, HiSeq X Ten, Illumina Genome Analyzer, Illumina Genome Analyzer II, Illumina Genome Analyzer IIx, Illumina HiScanSQ, Illumina HiSeq 1000, Illumina HiSeq 1500, Illumina HiSeq 2000, Illumina HiSeq 2500, Illumina HiSeq 3000, Illumina HiSeq 4000, Illumina HiSeq X, Illumina MiSeq, Illumina MiniSeq, Illumina NovaSeq 6000, Illumina NovaSeq X, Illumina iSeq 100, Ion GeneStudio S5, Ion GeneStudio S5 Plus, Ion GeneStudio S5 Prime, Ion Torrent Genexus, Ion Torrent PGM, Ion Torrent Proton, Ion Torrent S5, Ion Torrent S5 XL, MGISEQ-2000RS, MinION, NextSeq 1000, NextSeq 2000, NextSeq 500, NextSeq 550, Onso, PacBio RS, PacBio RS II, PromethION, Revio, Sentosa SQ301, Sequel, Sequel II, Sequel IIe, Tapestri, UG 100, unspecified

Run

A run contains a group of reads generated for a particular experiment.

Field name Cardinality Description Controlled vocabulary
alias mandatory Unique identificator for each run.
experiment_alias mandatory From_experiment_metadata
file_name mandatory The name or relative pathname of a run data file.
file_format mandatory The run data file model. sra, srf, sff, fastq, fasta, tab, 454_native, 454_native_seq, 454_native_qual, Helicos_native, Illumina_native, Illumina_native_seq, Illumina_native_prb, Illumina_native_int, Illumina_native_qseq, Illumina_native_scarf, SOLiD_native, SOLiD_native_csfasta, SOLiD_native_qual, PacBio_HDF5, bam, cram, CompleteGenomics_native, OxfordNanopore_native

Sample

A Sample defines an isolate of sequenceable material upon which sequencing experiments can be based. The Sample object may be a surrogate for taxonomy accession or an anonymized individual identifier. Or, it may fully specify provenance and isolation method of the starting material.

Field name Cardinality Description Controlled vocabulary
alias mandatory Unique identificator for each sample.
title mandatory Short text that can be used to call out sample records in search results or in displays.
taxon_id mandatory Ncbi taxonomy identifier. this is appropriate for individual organisms and some environmental samples.
sample_description optional Free-form text describing the sample, its origin, and its method of isolation.
number of inoculated individuals optional Number of host individuals inoculated for the experiment.
inoculation route optional Brief description of the protocol inoculation route.
inoculation dose optional Dose used for the inoculoation experiment.
inoculation stock availability optional Is the virus stock used for the inoculation available? no, yes
sample storage conditions optional Conditions at which sample was stored, usually storage temperature, duration and location. in soil context: explain how and for how long the soil sample was stored before dna extraction (fresh/frozen/other).
subject exposure optional Exposure of the subject to infected human or animals, such as poultry, wild bird or swine. if multiple exposures are applicable, please state them separated by semicolon. example: poultry; wild bird
type exposure optional Setting within which the subject is exposed to animals, such as farm, slaughterhouse, food preparation. if multiple exposures are applicable, please state their type in the same order in which you reported the exposure in the field 'subject exposure'. example: backyard flock; confined animal feeding operation
personal protective equipment optional Use of personal protective equipment, such as gloves, gowns, during any type of exposure. example: mask
hospitalisation optional Was the subject confined to a hospital as a result of virus infection or problems occurring secondary to virus infection? no, yes
antiviral treatment optional Antiviral treatment used for this subject, such as zanamavir oseltamivir, amantadine. example: rimantadine
antiviral treatment initiation optional Initiation of antiviral treatment after onset of clinical symptoms in days. example: 2.5
antiviral treatment dosage optional Dosage of the treatment taken by the subject. example: 0.05 mg
antiviral treatment duration optional Duration of antiviral treatment after onset of clinical symptoms in days.example: 5
influenza vaccination type optional Influenza vaccinations that have been administered to the subject over the last year. example: 2009 h1n1 flumist
influenza vaccination date optional Date that the influenza vaccination was administered to the subject over the past year. format: yyyy-mm-dd. example: 2007-05-12
source of vaccination information optional Designation of information related to vaccination history as self reported or documented. documented, self reported
vaccine lot number optional Lot number of the vaccine.
vaccine manufacturer optional Manufacturer of the vaccine.
vaccine dosage optional Dosage of the vaccine taken by the subject. example: 0.05 ml
influenza-like illness at the time of sample collection optional Is the subject at the time of sample collection considered to have influenza like illness? no, yes
illness onset date optional Date the subject showed an onset of symptoms. format: yyyy-mm-dd. example: 2011-10-20
illness duration optional The number of days the illness lasted. example: 4
illness symptoms optional The symptoms that have been reported in relation to the illness, such as cough, diarrhea, fever, headache, malaise, myalgia, nausea, runny_nose, shortness_of_breath, sore_throat. if multiple exposures are applicable, please state them separated by semicolon.
collection date mandatory The date the sample was collected with the intention of sequencing, either as an instance (single point in time) or interval. in case no exact time is available, the date/time can be right truncated i.e. all of these are valid iso8601 compliant times: 2008-01-23t19:23:10+00:00; 2008-01-23t19:23:10; 2008-01-23; 2008-01; 2008.
geographic location (latitude) recommended The geographical origin of the sample as defined by latitude. the values should be reported in decimal degrees and in wgs84 system (Units: DD)
geographic location (longitude) recommended The geographical origin of the sample as defined by longitude. the values should be reported in decimal degrees and in wgs84 system (Units: DD)
geographic location (region and locality) recommended The geographical origin of the sample as defined by the specific region name followed by the locality name.
subject exposure duration optional Duration of the exposure of the subject to an infected human or animal. if multiple exposures are applicable, please state their duration in the same order in which you reported the exposure in the field 'subject exposure'. example: 1 day; 0.33 days (Units: year)
sample capture status recommended Reason for the sample collection. active surveillance in response to outbreak, active surveillance not initiated by an outbreak, farm sample, market sample, other, pet sample, zoo sample
geographic location (country and/or sea) mandatory The geographical origin of where the sample was collected from, with the intention of sequencing, as defined by the country or sea name. country or sea names should be chosen from the insdc country list (http://insdc.org/country.html). Afghanistan, Albania, Algeria, American Samoa, Andorra, Angola, Anguilla, Antarctica, Antigua and Barbuda, Arctic Ocean, Argentina, Armenia, Aruba, Ashmore and Cartier Islands, Atlantic Ocean, Australia, Austria, Azerbaijan, Bahamas, Bahrain, Baker Island, Baltic Sea, Bangladesh, Barbados, Bassas da India, Belarus, Belgium, Belize, Benin, Bermuda, Bhutan, Bolivia, Borneo, Bosnia and Herzegovina, Botswana, Bouvet Island, Brazil, British Virgin Islands, Brunei, Bulgaria, Burkina Faso, Burundi, Cambodia, Cameroon, Canada, Cape Verde, Cayman Islands, Central African Republic, Chad, Chile, China, Christmas Island, Clipperton Island, Cocos Islands, Colombia, Comoros, Cook Islands, Coral Sea Islands, Costa Rica, Cote d'Ivoire, Croatia, Cuba, Curacao, Cyprus, Czechia, Czech Republic, Democratic Republic of the Congo, Denmark, Djibouti, Dominica, Dominican Republic, East Timor, Ecuador, Egypt, El Salvador, Equatorial Guinea, Eritrea, Estonia, Ethiopia, Europa Island, Falkland Islands (Islas Malvinas), Faroe Islands, Fiji, Finland, France, French Guiana, French Polynesia, French Southern and Antarctic Lands, Gabon, Gambia, Gaza Strip, Georgia, Germany, Ghana, Gibraltar, Glorioso Islands, Greece, Greenland, Grenada, Guadeloupe, Guam, Guatemala, Guernsey, Guinea, Guinea-Bissau, Guyana, Haiti, Heard Island and McDonald Islands, Honduras, Hong Kong, Howland Island, Hungary, Iceland, India, Indian Ocean, Indonesia, Iran, Iraq, Ireland, Isle of Man, Israel, Italy, Jamaica, Jan Mayen, Japan, Jarvis Island, Jersey, Johnston Atoll, Jordan, Juan de Nova Island, Kazakhstan, Kenya, Kerguelen Archipelago, Kingman Reef, Kiribati, Kosovo, Kuwait, Kyrgyzstan, Laos, Latvia, Lebanon, Lesotho, Liberia, Libya, Liechtenstein, Lithuania, Luxembourg, Macau, Macedonia, Madagascar, Malawi, Malaysia, Maldives, Mali, Malta, Marshall Islands, Martinique, Mauritania, Mauritius, Mayotte, Mediterranean Sea, Mexico, Micronesia, Midway Islands, Moldova, Monaco, Mongolia, Montenegro, Montserrat, Morocco, Mozambique, Myanmar, Namibia, Nauru, Navassa Island, Nepal, Netherlands, New Caledonia, New Zealand, Nicaragua, Niger, Nigeria, Niue, Norfolk Island, North Korea, North Sea, Northern Mariana Islands, Norway, Oman, Pacific Ocean, Pakistan, Palau, Palmyra Atoll, Panama, Papua New Guinea, Paracel Islands, Paraguay, Peru, Philippines, Pitcairn Islands, Poland, Portugal, Puerto Rico, Qatar, Republic of the Congo, Reunion, Romania, Ross Sea, Russia, Rwanda, Saint Helena, Saint Kitts and Nevis, Saint Lucia, Saint Pierre and Miquelon, Saint Vincent and the Grenadines, Samoa, San Marino, Sao Tome and Principe, Saudi Arabia, Senegal, Serbia, Seychelles, Sierra Leone, Singapore, Sint Maarten, Slovakia, Slovenia, Solomon Islands, Somalia, South Africa, South Georgia and the South Sandwich Islands, South Korea, Southern Ocean, Spain, Spratly Islands, Sri Lanka, Sudan, Suriname, Svalbard, Swaziland, Sweden, Switzerland, Syria, Taiwan, Tajikistan, Tanzania, Tasman Sea, Thailand, Togo, Tokelau, Tonga, Trinidad and Tobago, Tromelin Island, Tunisia, Turkey, Turkmenistan, Turks and Caicos Islands, Tuvalu, USA, Uganda, Ukraine, United Arab Emirates, United Kingdom, Uruguay, Uzbekistan, Vanuatu, Venezuela, Viet Nam, Virgin Islands, Wake Island, Wallis and Futuna, West Bank, Western Sahara, Yemen, Zambia, Zimbabwe, missing, missing: control sample, missing: data agreement established pre-2023, missing: endangered species, missing: human-identifiable, missing: lab stock, missing: sample group, missing: synthetic construct, missing: third party data, not applicable, not collected, not provided, restricted access
host disease outcome recommended Disease outcome in the host. dead, recovered, recovered with sequelae
host common name mandatory Common name of the host, e.g. human
host subject id mandatory A unique identifier by which each subject can be referred to, de-identified, e.g. #131
host age recommended Age of host at the time of sampling; relevant scale depends on species and study, e.g. could be seconds for amoebae or centuries for trees (Units: years)
host health state mandatory Health status of the host at the time of sample collection. diseased, healthy, missing: control sample, missing: data agreement established pre-2023, missing: endangered species, missing: human-identifiable, missing: lab stock, missing: sample group, missing: synthetic construct, missing: third party data, not applicable, not collected, not provided, restricted access
host sex mandatory Gender or sex of the host. female, hermaphrodite, male, missing: control sample, missing: data agreement established pre-2023, missing: endangered species, missing: human-identifiable, missing: lab stock, missing: sample group, missing: synthetic construct, missing: third party data, neuter, not applicable, not collected, not provided, other, restricted access
host scientific name mandatory Scientific name of the natural (as opposed to laboratory) host to the organism from which sample was obtained.
influenza test method mandatory Method by which the current assessment of a sample as flu positive/negative is made. if multiple test were performed, please state them separated by semicolon. example: rt-pcr; antigen elisa
influenza test result mandatory Classification of a sample as flu positive or negative based on the test performed and reported. if multiple tests were performed, please state the results in the same order in which you reported the tests in the field 'influenza test method'. format: p(ositive)/n(egative). example: p; p
other pathogens tested mandatory Classification of pathogenic organisms other than influenza virus tested in the current assessment of a sample. if multiple tests were performed, please state them separated by semicolon. if no other pathogens test was performed, please state 'none'. example: newcastle
other pathogens test result mandatory Classification of a sample as positive or negative based on the test performed and reported. if tests for multiple pathogenic organisms were performed, please state the results in the same order in which you reported the tests in the field 'other pathogens tested'. format: p(ositive)/n(egative)/not applicable. example: n
influenza virus type recommended One of the three influenza virus classification types. A, B, C
virus identifier recommended Unique laboratory identifier assigned to the virus by the investigator. strain name is not sufficient since it might not be unique due to various passsages of the same virus. format: up to 50 alphanumeric characters
influenza strain unique number optional Unique number of the strain which is reported as a part of the influenza strain name, such as a/chicken/fujian/411/2002(hxn1). format: integer number, example: 411
WHO/OIE/FAO clade (required for HPAI H5N1 viruses) optional Who/oie/fao clade should be included for highly pathogenic h5n1 viruses. example: 2.2
lineage:swl (required for H1N1 viruses) optional Does the h1n1 influenza virus originate from a swine-like outbreak (as opposed to a seasonal flu)? no, yes
collector name mandatory Name of the person who collected the specimen. example: john smith
collecting institution mandatory Name of the institution to which the person collecting the specimen belongs. format: institute name, institute address
receipt date recommended Date on which the sample was received. format:yyyy-mm-dd. please provide the highest precision possible. if the sample was received by the institution and not collected, the 'receipt date' must be provided instead. either the 'collection date' or 'receipt date' must be provided. if available, provide both dates.
definition for seropositive sample recommended The cut off value used by an investigatior in determining that a sample was seropositive.
meaning of cut off value optional Description helping to explain what the cut off value means.
serotype (required for a seropositive sample) recommended Serological variety of a species characterised by its antigenic properties. for influenza, ha subtype should be the letter h followed by a number between 1-16 unless novel subtype is identified and the na subtype should be the letter n followed by a number between 1-9 unless novel subtype is identified. if only one of the subtypes have been tested then use the format h5nx or hxn1. example: h1n1
strain optional Name of the strain from which the sample was obtained.
host habitat recommended Natural habitat of the avian or mammalian host. domestic:free-range farm, domestic:indoor farm, domestic:live market, domestic:semi-enclosed housing, other, wild:migratory, wild:resident
isolation source host-associated recommended Name of host tissue or organ sampled for analysis. example: tracheal tissue
host description optional Other descriptive information relating to the host.
gravidity optional Whether or not the subject is gravid. if so, report date due or date post-conception and specify which of these two dates is being reported.
host behaviour recommended Natural behaviour of the host. captive-wild (e.g. at zoo), domestic, other, wild
isolation source non-host-associated recommended Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived. example: soil