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Sample workflow output

Run Summary

The run_summary.py script outputs a run metrics json and html file. This is similar to what you would get in the Run Summary tab in the SAV Viewer software.

run_metrics.html

run_metrics.json

% Base

This plots the base % across each cycle. Each line represents a different base. Reference lines are added for each read.

File: percent_base.svg

percent base plot

Tile intensity

This plots the combined tile intensity for each lane.

File: max_intensity_1.svg

tile intensity

% Occupancy (NovaSeq only)

The % Occupied and % Pass Filter metrics are plotted to determine if a run was underloaded, optimally loaded, or overloaded.

More information found here.

Demo dataset

Download the MiSeqDemo.zip dataset here.