From 6b73a9df79e37c398d7e438d883cdc00f57aaea2 Mon Sep 17 00:00:00 2001
From: Katharina Hoff <39333668+KatharinaHoff@users.noreply.github.com>
Date: Thu, 7 Dec 2023 21:23:34 +0100
Subject: [PATCH] Update long_read_protocol.md
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# Long-Read Integration Using BRAKER, GeneMarkS-T and TSEBRA
+> :warning: Parts of this protocol are outdated (in particular, the cupcake part). We will likely not update this protocol.
+
## Introduction
The following instructions are a prelimary protocol for producing a BRAKER[1](#ref1)[2](#ref2)[3](#ref3) gene set that integrates extrinsic evidence from long-read RNA-Seq (PacBio ccs), short-read RNA-seq (Illumina) and a large database of protein sequences (e.g. OrthoDB [9](#ref9) clade) into a single prediction.
-> :warning: This protocol was developed for presentation of intermediate results at **The Plant and Animal Genome Conference XXIX** (2022) in San Diego. Significant changes are expected prior publication in a peer reviewed scientific journal. For preliminary accuracy results, see https://github.com/Gaius-Augustus/BRAKER/blob/master/docs/slides/slides_PAG2022.pdf (will be made available after January 9th 2022).
+> :warning: This protocol was developed for presentation of intermediate results at **The Plant and Animal Genome Conference XXIX** (2022) in San Diego. Significant changes are expected prior publication in a peer reviewed scientific journal. For preliminary accuracy results, see https://github.com/Gaius-Augustus/BRAKER/blob/master/docs/slides/slides_PAG2022.pdf.
## Installation