diff --git a/README.md b/README.md index 7d6d542..6901abf 100644 --- a/README.md +++ b/README.md @@ -97,33 +97,33 @@ The human GRCh38 [reference transcriptome](https://ftp.ensembl.org/pub/release-1 ##### BASIC WORKFLOW ```bash -# nextflow run main.nf --matrix dataset/sub_pbmc_matrice.csv \ -# --transcriptome dataset/Homo_sapiens.GRCh38.cdna.all.fa \ -# --features gene_name \ -# --gtf dataset/genes.gtf + nextflow run main.nf --matrix dataset/sub_pbmc_matrice.csv \ + --transcriptome dataset/Homo_sapiens.GRCh38.cdna.all.fa \ + --features gene_name \ + --gtf dataset/genes.gtf ``` ##### WITH PCR AMPLIFICTION ```bash -# nextflow run main.nf --matrix dataset/sub_pbmc_matrice.csv \ -# --transcriptome dataset/Homo_sapiens.GRCh38.cdna.all.fa \ -# --features gene_name \ -# --gtf dataset/GRCh38-2020-A-genes.gtf \ -# --pcr_cycles 2 \ -# --pcr_dup_rate 0.7 \ -# --pcr_error_rate 0.00003 + nextflow run main.nf --matrix dataset/sub_pbmc_matrice.csv \ + --transcriptome dataset/Homo_sapiens.GRCh38.cdna.all.fa \ + --features gene_name \ + --gtf dataset/GRCh38-2020-A-genes.gtf \ + --pcr_cycles 2 \ + --pcr_dup_rate 0.7 \ + --pcr_error_rate 0.00003 ``` ##### WITH SIMULATED CELL TYPE COUNTS ```bash -# nextflow run main.nf --matrix dataset/sub_pbmc_matrice.csv \ -# --transcriptome dataset/Homo_sapiens.GRCh38.cdna.all.fa \ -# --features gene_name \ -# --gtf dataset/GRCh38-2020-A-genes.gtf \ -# --sim_celltypes true \ -# --cell_types_annotation dataset/sub_pbmc_cell_type.csv + nextflow run main.nf --matrix dataset/sub_pbmc_matrice.csv \ + --transcriptome dataset/Homo_sapiens.GRCh38.cdna.all.fa \ + --features gene_name \ + --gtf dataset/GRCh38-2020-A-genes.gtf \ + --sim_celltypes true \ + --cell_types_annotation dataset/sub_pbmc_cell_type.csv ``` ##### WITH PERSONALIZED ERROR MODEL @@ -141,18 +141,18 @@ nextflow run main.nf --matrix dataset/sub_pbmc_matrice.csv \ ##### COMPLETE WORKFLOW ```bash -# nextflow run main.nf --matrix dataset/sub_pbmc_matrice.csv \ -# --transcriptome dataset/Homo_sapiens.GRCh38.cdna.all.fa \ -# --features gene_name \ -# --gtf dataset/GRCh38-2020-A-genes.gtf \ -# --sim_celltypes true \ -# --cell_types_annotation dataset/sub_pbmc_cell_type.csv -# --build_model true \ -# --fastq_model dataset/sub_pbmc_reads.fq \ -# --ref_genome dataset/GRCh38-2020-A-genome.fa -# --pcr_cycles 2 \ -# --pcr_dup_rate 0.7 \ -# --pcr_error_rate 0.00003 + nextflow run main.nf --matrix dataset/sub_pbmc_matrice.csv \ + --transcriptome dataset/Homo_sapiens.GRCh38.cdna.all.fa \ + --features gene_name \ + --gtf dataset/GRCh38-2020-A-genes.gtf \ + --sim_celltypes true \ + --cell_types_annotation dataset/sub_pbmc_cell_type.csv + --build_model true \ + --fastq_model dataset/sub_pbmc_reads.fq \ + --ref_genome dataset/GRCh38-2020-A-genome.fa + --pcr_cycles 2 \ + --pcr_dup_rate 0.7 \ + --pcr_error_rate 0.00003 ``` ## Results