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Dear all,
I just created a NextFlow pipeline called nf-m6anet, which performs alignment of sequencing reads in fastq format to the transcriptome with minimap2, resquiggling of raw fast5 files with f5c re-implementation of Nanopolish and m6A detection with m6anet. Compared to nanoseq, it also filters high-quality m6A+ sites based on modification probability and performs lift-over of transcriptome-based to genome-based coordinates. Feel free to use it and provide me with any feedbacks or suggestions you may have!
Best
This discussion was converted from issue #68 on December 23, 2022 01:44.
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Dear all,
I just created a NextFlow pipeline called nf-m6anet, which performs alignment of sequencing reads in fastq format to the transcriptome with minimap2, resquiggling of raw fast5 files with f5c re-implementation of Nanopolish and m6A detection with m6anet. Compared to nanoseq, it also filters high-quality m6A+ sites based on modification probability and performs lift-over of transcriptome-based to genome-based coordinates. Feel free to use it and provide me with any feedbacks or suggestions you may have!
Best
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