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k18-hACE2-coinfection-transcriptomics

Sequential infection with influenza A virus followed by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) leads to more severe disease and encephalitis in a mouse model of COVID-19.

Jordan J. Clark^1†, Rebekah Penrice-Randal^1†, Parul Sharma¹, Anja Kipar^1,2, Xiaofeng Dong¹, Shaun H. Pennington³, Amy E. Marriott³, Stefano Colombo³, Andrew Davidson⁴, Maia Kavanagh Williamson⁴, David A. Matthews⁴, Lance Turtle^5,6, Tessa Prince¹, Grant L. Hughes⁷, Edward I. Patterson⁷, Ghada Shawli¹, Daniele F. Mega¹, Krishanthi Subramaniam¹, Jo Sharp⁸, Lynn McLaughlin⁹, En-Min Zhou^1,10, Joseph D. Turner³, Giancarlo Biagini³, Andrew Owen⁸, Julian A. Hiscox^1,10,11, James P. Stewart^1,10,12

¹Department of Infection Biology & Microbiomes, Institute of Infection, Veterinary and Ecological Sciences, University of Liverpool, UK.

²Laboratory for Animal Model Pathology, Institute of Veterinary Pathology, University of Zurich, Switzerland.

³Department of Tropical Disease Biology, Centre for Drugs and Diagnostics, Liverpool School of Tropical Medicine, Liverpool, UK.

⁴School of Cellular and Molecular Medicine, Faculty of Life Sciences, University of Bristol, Bristol, UK.

⁵Department of Clinical Infection Microbiology and Immunology and NIHR Health Protection Research Unit for Emerging and Zoonotic Infections, Institute of Infection, Veterinary and Ecological Sciences, University of Liverpool, UK.

⁶Tropical & Infectious Disease Unit, Royal Liverpool University Hospital

⁷Departments of Vector Biology and Tropical Disease Biology, Centre for Neglected Tropical Disease, Liverpool School of Tropical Medicine, Liverpool, UK.

⁸Department of Pharmacology and Therapeutics, Centre of Excellence in Long-acting Therapeutics (CELT), University of Liverpool, UK.

⁹Biomedical Services Unit, University of Liverpool, UK.

¹⁰Department of Preventive Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, China.

¹¹Infectious Diseases Horizontal Technology Centre (ID HTC), A*STAR, Singapore.

¹²Department of Infectious Disease, University of Georgia, Georgia, USA.

*Corresponding authors: E. mail: [email protected]; [email protected]

^†These authors contributed equally to this work

Preprint

https://www.biorxiv.org/content/10.1101/2020.10.13.334532v3

Data availability

Sequencing reads have been uploaded to bioproject:

PRJNA914664 : K18-hACE2 mice, SARS-CoV-2 and IAV coinfection transcriptomics