diff --git a/robotools/evotools/test_worklist.py b/robotools/evotools/test_worklist.py
index 8a58f69..22e997b 100644
--- a/robotools/evotools/test_worklist.py
+++ b/robotools/evotools/test_worklist.py
@@ -1,6 +1,7 @@
 import logging
 import os
 import tempfile
+from pathlib import Path
 
 import numpy as np
 import pytest
@@ -268,6 +269,17 @@ def test_aspirate_systemliquid(self) -> None:
             assert wl[-1] == "A;Systemliquid;;;1;;200.00;;;;"
         return
 
+    def test_accepts_path(self):
+        fp = Path(tempfile.gettempdir(), os.urandom(24).hex() + ".gwl")
+        try:
+            with Worklist(fp) as wl:
+                wl.comment("Test")
+            assert isinstance(wl._filepath, Path)
+            assert fp.exists()
+        finally:
+            fp.unlink(missing_ok=True)
+        return
+
     def test_save(self) -> None:
         tf = tempfile.mktemp() + ".gwl"
         error = None
diff --git a/robotools/evotools/worklist.py b/robotools/evotools/worklist.py
index 5e46d7a..90810fa 100644
--- a/robotools/evotools/worklist.py
+++ b/robotools/evotools/worklist.py
@@ -3,9 +3,9 @@
 import collections
 import logging
 import math
-import os
 import typing
 import warnings
+from pathlib import Path
 from typing import Dict, List, Optional, Tuple, Union
 
 import numpy
@@ -277,7 +277,7 @@ class Worklist(list):
 
     def __init__(
         self,
-        filepath: Optional[str] = None,
+        filepath: Optional[Union[str, Path]] = None,
         max_volume: typing.Union[int, float] = 950,
         auto_split: bool = True,
     ) -> None:
@@ -285,7 +285,7 @@ def __init__(
 
         Parameters
         ----------
-        filepath : str
+        filepath
             Optional filename/filepath to write when the context is exited (must include a .gwl extension)
         max_volume : int
             Maximum aspiration volume in µL
@@ -293,7 +293,9 @@ def __init__(
             If `True`, large volumes in transfer operations are automatically splitted.
             If set to `False`, `InvalidOperationError` is raised when a pipetting volume exceeds `max_volume`.
         """
-        self._filepath = filepath
+        self._filepath: Optional[Path] = None
+        if filepath is not None:
+            self._filepath = Path(filepath)
         if max_volume is None:
             raise ValueError("The `max_volume` parameter is required.")
         self.max_volume = max_volume
@@ -309,17 +311,17 @@ def __exit__(self, exc_type, exc_val, exc_tb) -> None:
             self.save(self._filepath)
         return
 
-    def save(self, filepath: str) -> None:
+    def save(self, filepath: Union[str, Path]) -> None:
         """Writes the worklist to the filepath.
 
         Parameters
         ----------
-        filepath : str
+        filepath
             File name or path to write (must include a .gwl extension)
         """
-        assert ".gwl" in filepath.lower(), "The filename did not contain the .gwl extension."
-        if os.path.exists(filepath):
-            os.remove(filepath)
+        filepath = Path(filepath)
+        assert ".gwl" in filepath.name.lower(), "The filename did not contain the .gwl extension."
+        filepath.unlink(missing_ok=True)
         with open(filepath, "w", newline="\r\n", encoding="latin_1") as file:
             file.write("\n".join(self))
         return