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VisProt for differential visual proteomics by electron microscopy

About:

VisProt is a MATLAB-based package designed for electron microscopy image-prosessing and differential visual proteomics.

Instructions:

  1. Create a project directory and add the vp package.

  2. In the project directory create the folder RawMicrographs that contains micrographs of different samples (eg: RawMicrographs/Sample1/, RawMicrographs/Sample2/, etc).

  3. Launch MATLAB in the project directory and initiate VisProt with either:

vp.MasterScript_matlab

for particle picking with MATLAB (.tif micrographs) or

vp.MasterScript_gauto

for particle picking with Gautomatch (.mrc micrographs).

Dependencies:

  • Supported platforms: MacOS/Linux
  • Matlab 9.0 (R2016a) or later
  • MATLAB Central File Exchange:
    1. Cobeldick, Stephen (2012). Natural-Order Filename Sort
    2. Sigworth, Fred (2009). Imagic, MRC and DM3 file i/o
    3. Altman, Yair (2007). Findjobj - Find java handles of Matlab graphic objects
  • Relion-2.1
  • Gautomatch v0.53
  • CTFFIND-4.1.4