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Currently, **Ninetails** can distinguish characteristic signatures of four types of nucleotides: adenosines (A), cytosines (C), guanosines (G), and uridines (U).
-**Ninetails** relies on Nanopolish segmentation and therefore may underestimate terminal modifications (last and penultimate nucleotides of the tail).
-
> **Note**
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>
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+> **Note**
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+>**Ninetails** relies on Nanopolish segmentation and therefore may underestimate terminal modifications (last and penultimate nucleotides of the tail).
+>
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## Citation
Please cite **Ninetails** as: Gumińska N., Matylla-Kulińska K., Krawczyk P., Orzeł W., Maj M., Mroczek S., Dziembowski A. Direct detection of non-adenosine nucleotides within poly(A) tails -- a new tool for the analysis of post-transcriptional mRNA tailing, 27th Annual Meeting of the RNA Society, Boulder, Colorado, May 31 to June 5, 2022.