@@ -92,7 +92,12 @@ public void printUsageInfo() {
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System .out .println (example );
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System .out .println ();
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- System .out .println ("Documentation: https://github.com/MSGFPlus/msgfplus" );
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+ System .out .println ();
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+ System .out .println ("For Thermo .raw files, obtain a centroided .mzML file using MSConvert, which is part of ProteoWizard (http://proteowizard.sourceforge.net/)" );
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+ System .out .println (" MSConvert.exe DatasetName.raw --filter \" peakPicking true 1-\" --mzML --32" );
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+ System .out .println ();
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+ System .out .println ("Documentation: https://msgfplus.github.io/msgfplus/" );
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+ System .out .println ("Releases: https://github.com/MSGFPlus/msgfplus/releases" );
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}
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public void printValues () {
@@ -150,6 +155,7 @@ public void addSpecFileParam() {
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specFileParam .addFileFormat (SpecFileFormat .DTA_TXT );
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specFileParam .addFileFormat (FileFormat .DIRECTORY );
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specFileParam .fileMustExist ();
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+ specFileParam .setAdditionalDescription ("Spectra should be centroided (see below for MSConvert example). Profile spectra will be ignored." );
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addParameter (specFileParam );
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}
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@@ -372,8 +378,8 @@ public void addMSGFPlusParams() {
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maxMissedCleavages .defaultValue (-1 );
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addParameter (maxMissedCleavages );
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- addExample ("Example (high-precision): java -Xmx3500M -jar MSGFPlus.jar -s test.mzML -d IPI_human_3.79.fasta -inst 1 -t 20ppm -ti -1,2 -ntt 2 -tda 1 -o testMSGFPlus.mzid" );
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- addExample ("Example (low-precision): java -Xmx3500M -jar MSGFPlus.jar -s test.mzML -d IPI_human_3.79.fasta -inst 0 -t 0.5Da,2.5Da -ntt 2 -tda 1 -o testMSGFPlus.mzid" );
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+ addExample ("Example (high-precision): java -Xmx3500M -jar MSGFPlus.jar -s test.mzML -d IPI_human_3.79.fasta -inst 1 -t 20ppm -ti -1,2 -ntt 2 -tda 1 -o testMSGFPlus.mzid -mod Mods.txt " );
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+ addExample ("Example (low-precision): java -Xmx3500M -jar MSGFPlus.jar -s test.mzML -d IPI_human_3.79.fasta -inst 0 -t 0.5Da,2.5Da -ntt 2 -tda 1 -o testMSGFPlus.mzid -mod Mods.txt " );
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// Hidden parameters
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FileParameter dbIndexDirParam = new FileParameter ("dd" , "DBIndexDir" , "Path to the directory containing database index files" );
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