Troubleshooting Spike Sorting Issues in Kilosort4 with High Noise Data

[mamang1101]

Feature you'd like to see:

"Hi, I’m currently a Ph.D. student who has just started experimenting. Since I’m new to spike sorting with Kilosort4, there are still many things I don’t fully understand, so I hope you can bear with me.

I’ve converted the .tev file obtained from TNT into a .bin file and proceeded with spike sorting using Kilosort4. My data has a characteristic where there’s significant noise (caused by the mouse licking), which persists continuously. However, as you can see in the figure below, when I check the overall recording data using NeuroExplorer, clear spikes are consistently present. When I sort this data using OfflineSorter, it seems to sort well, so I currently assume that the issue is not with the data itself.

However, when I process this data in Kilosort4 and check it in Phy, as you can see, it doesn’t seem to sort properly. Even when I look at the waveforms, they don’t look like spikes at all.

If the significant noise in my data is causing these results in Kilosort4, is there any way to address this?
I ran Kilosort4 with the settings below. Are there any parameters I should consider changing?"

Thank you for reading, and I hope everyone gets lots of 'good'

Additional Context

No response

[jacobpennington]

Please start by uploading kilosort4.log from the results directory.