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Rxn_ICI.xml
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Rxn_ICI.xml
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<?xml version="1.0"?>
<ReactionScheme>
<!-- you can specify the units of the diffusion constant. If they are not specified they are assumed to be micron^2/second, (microns written "mu" here) it should understand microns, mm, and m for distances and seconds and ms for times -->
<!-- This code is implemented CaMKII_bimols Rns -0.44, 0.28, 0.088. Fix 3 products 2rxns break down to 2nd order rxn : LbARG/Ip35P1P2 -->
<!-- last rxn of Ip35p1p2 was wrong reactant:fixed on Aug,8th,2008
Chosen katp=1 sec(not 10 sec) is enough -->
<!-- Suggested by avrama Ca kdiff=600 for helping fast decay on Feb.24th 2009-->
<Specie name="ICI" id="ICI" kdiff="91.4" kdiffunit="mu2/s"/>
<Specie name="ICIOut" id="ICIOut" kdiff="10.5"
kdiffunit="mu2/s"/>
<Specie name="ICIbAR" id="ICIbAR" kdiff="0" kdiffunit="mu2/s"/>
<Specie name="Ca" id="Ca" kdiff="174.3" kdiffunit="mu2/s" />
<Specie name="CaOut" id="CaOut" kdiff="3." kdiffunit="mu2/s" />
<!--Added from Andy's model-->
<Specie name="CaOutLeak" id="CaOutLeak" kdiff="0"
kdiffunit="mu2/s" />
<Specie name="CaCbuf" id="CaCbuf" kdiff="10" kdiffunit="mu2/s"/>
<Specie name="Cbuf" id="Cbuf" kdiff="10" kdiffunit="mu2/s"/>
<Specie name="Leak" id="Leak" kdiff="0" kdiffunit="mu2/s" />
<Specie name="Calbin" id="Calbin" kdiff="9.3" kdiffunit="mu2/s" />
<Specie name="CalbinC" id="CalbinC" kdiff="9.3" kdiffunit="mu2/s" />
<Specie name="CaBCa" id="CaBCa" kdiff="10" kdiffunit="mu2/s"/>
<Specie name="CaB" id="CaB" kdiff="10" kdiffunit="mu2/s"/>
<!--Added from Andy's model-->
<Specie name="ISOOut" id="ISOOut" kdiff="10." kdiffunit="mu2/s" />
<Specie name="Ibuf" id="Ibuf" kdiff="1" kdiffunit="mu2/s" />
<Specie name="ISOIbuf" id="ISOIbuf" kdiff="0" kdiffunit="mu2/s" />
<Specie name="Epac1" id="Epac1" kdiff="5.61" kdiffunit="mu2/s" />
<Specie name="Epac1cAMP" id="Epac1cAMP" kdiff="5.62"
kdiffunit="mu2/s" />
<Specie name="pmca" id="pmca" kdiff="0" kdiffunit="mu2/s" />
<Specie name="ncx" id="ncx" kdiff="0" kdiffunit="mu2/s" />
<Specie name="pmcaCa" id="pmcaCa" kdiff="0" kdiffunit="mu2/s" />
<Specie name="ncxCa" id="ncxCa" kdiff="0" kdiffunit="mu2/s" />
<Specie name="ISO" id="ISO" kdiff="111.4" kdiffunit="mu2/s" />
<!--Added from Andy's model-->
<Specie name="bAR" id="bAR" kdiff="0" kdiffunit="mu2/s" />
<Specie name="Gs" id="Gs" kdiff="0" kdiffunit="mu2/s" />
<Specie name="Gi" id="Gi" kdiff="0" kdiffunit="mu2/s" />
<Specie name="ISObAR" id="ISObAR" kdiff="0" kdiffunit="mu2/s" />
<Specie name="ISObARGs" id="ISObARGs" kdiff="0"
kdiffunit="mu2/s" />
<Specie name="PKAcISObAR" id="PKAcISObAR" kdiff="0"
kdiffunit="mu2/s" />
<Specie name="PKAcpISObAR" id="PKAcpISObAR" kdiff="0"
kdiffunit="mu2/s" />
<Specie name="PKAcppISObAR" id="PKAcppISObAR" kdiff="0"
kdiffunit="mu2/s" />
<Specie name="PKAcpppISObAR" id="PKAcpppISObAR" kdiff="0"
kdiffunit="mu2/s" />
<Specie name="pISObAR" id="pISObAR" kdiff="0" kdiffunit="mu2/s" />
<Specie name="ppISObAR" id="ppISObAR" kdiff="0"
kdiffunit="mu2/s" />
<Specie name="pppISObAR" id="pppISObAR" kdiff="0"
kdiffunit="mu2/s" />
<Specie name="ppppISObAR" id="ppppISObAR" kdiff="0"
kdiffunit="mu2/s" />
<Specie name="ppppISObARGi" id="ppppISObARGi" kdiff="0"
kdiffunit="mu2/s" />
<Specie name="PKAcbAR" id="PKAcbAR" kdiff="0" kdiffunit="mu2/s" />
<Specie name="PKAcpbAR" id="PKAcpbAR" kdiff="0"
kdiffunit="mu2/s" />
<Specie name="PKAcppbAR" id="PKAcppbAR" kdiff="0"
kdiffunit="mu2/s" />
<Specie name="PKAcpppbAR" id="PKAcpppbAR" kdiff="0"
kdiffunit="mu2/s" />
<Specie name="pbAR" id="pbAR" kdiff="0" kdiffunit="mu2/s" />
<Specie name="ppbAR" id="ppbAR" kdiff="0" kdiffunit="mu2/s" />
<Specie name="pppbAR" id="pppbAR" kdiff="0" kdiffunit="mu2/s" />
<Specie name="ppppbAR" id="ppppbAR" kdiff="0" kdiffunit="mu2/s" />
<Specie name="ppppbARGi" id="ppppbARGi" kdiff="0"
kdiffunit="mu2/s" />
<Specie name="GsbAR" id="GsbAR" kdiff="0" kdiffunit="mu2/s" />
<Specie name="GasGTP" id="GasGTP" kdiff="0" kdiffunit="mu2/s" />
<Specie name="GasGDP" id="GasGDP" kdiff="0" kdiffunit="mu2/s" />
<Specie name="GaiGTP" id="GaiGTP" kdiff="0" kdiffunit="mu2/s" />
<Specie name="GaiGDP" id="GaiGDP" kdiff="0" kdiffunit="mu2/s" />
<Specie name="Gibg" id="Gibg" kdiff="0" kdiffunit="mu2/s" />
<Specie name="Gsbg" id="Gsbg" kdiff="0" kdiffunit="mu2/s" />
<Specie name="Da" id="Da" kdiff="10" kdiffunit="mu2/s" />
<Specie name="Dbuf" id="Dbuf" kdiff="1" kdiffunit="mu2/s" />
<Specie name="DaDbuf" id="DaDbuf" kdiff="0" kdiffunit="mu2/s" />
<Specie name="DaOut" id="DaOut" kdiff="1" kdiffunit="mu2/s" />
<Specie name="D1R" id="D1R" kdiff="0" kdiffunit="mu2/s" />
<Specie name="DaD1R" id="DaD1R" kdiff="0" kdiffunit="mu2/s" />
<Specie name="DaD1RGs" id="DaD1RGs" kdiff="0" kdiffunit="mu2/s" />
<Specie name="GsD1R" id="GsD1R" kdiff="0" kdiffunit="mu2/s" />
<Specie name="AC1" id="AC1" kdiff="0" kdiffunit="mu2/s" />
<Specie name="AC1GasGTP" id="AC1GasGTP" kdiff="0"
kdiffunit="mu2/s" />
<Specie name="AC1GasGTPCaMCa4" id="AC1GasGTPCaMCa4" kdiff="0"
kdiffunit="mu2/s" />
<Specie name="AC1GasGTPCaMCa4ATP" id="AC1GasGTPCaMCa4ATP" kdiff="0"
kdiffunit="mu2/s" />
<Specie name="AC1GaiGTP" id="AC1GaiGTP" kdiff="0"
kdiffunit="mu2/s" />
<Specie name="AC1GaiGTPCaMCa4" id="AC1GaiGTPCaMCa4" kdiff="0"
kdiffunit="mu2/s" />
<Specie name="AC1GaiGTPCaMCa4ATP" id="AC1GaiGTPCaMCa4ATP" kdiff="0"
kdiffunit="mu2/s" />
<Specie name="AC1GasGTPGaiGTP" id="AC1GasGTPGaiGTP" kdiff="0"
kdiffunit="mu2/s" />
<Specie name="AC1GasGTPGaiGTPCaMCa4" id="AC1GasGTPGaiGTPCaMCa4"
kdiff="0" kdiffunit="mu2/s" />
<Specie name="AC1GsGiCaMCa4ATP" id="AC1GsGiCaMCa4ATP" kdiff="0"
kdiffunit="mu2/s" />
<Specie name="ATP" id="ATP" kdiff="1" kdiffunit="mu2/s" />
<Specie name="cAMP" id="cAMP" kdiff="86.4" kdiffunit="mu2/s" />
<Specie name="AC1CaMCa4" id="AC1CaMCa4" kdiff="0"
kdiffunit="mu2/s" />
<Specie name="AC1CaMCa4ATP" id="AC1CaMCa4ATP" kdiff="0"
kdiffunit="mu2/s" />
<Specie name="AC8" id="AC8" kdiff="0" kdiffunit="mu2/s" />
<Specie name="AC8CaMCa4" id="AC8CaMCa4" kdiff="0"
kdiffunit="mu2/s" />
<Specie name="AC8CaMCa4ATP" id="AC8CaMCa4ATP" kdiff="0"
kdiffunit="mu2/s" />
<Specie name="PDE1" id="PDE1" kdiff="0" kdiffunit="mu2/s" />
<Specie name="PDE1CaMCa4" id="PDE1CaMCa4" kdiff="0"
kdiffunit="mu2/s" />
<Specie name="PDE1CaMCa4cAMP" id="PDE1CaMCa4cAMP" kdiff="0"
kdiffunit="mu2/s" />
<Specie name="AMP" id="AMP" kdiff="84.8" kdiffunit="mu2/s" />
<Specie name="Ng" id="Ng" kdiff="0" kdiffunit="mu2/s" />
<Specie name="NgCaM" id="NgCaM" kdiff="0" kdiffunit="mu2/s" />
<!-- -->
<Specie name="CaM" id="CaM" kdiff="4" kdiffunit="mu2/s" />
<!--Calmodulin-->
<Specie name="CaMCa2" id="CaMCa2" kdiff="4" kdiffunit="mu2/s" />
<!-- 2 Ca bound Calmodulin-->
<Specie name="CaMCa4" id="CaMCa4" kdiff="4" kdiffunit="mu2/s" />
<!-- 4 Ca bound Calmodulin-->
<!-- -->
<Specie name="PP2B" id="PP2B" kdiff="0" kdiffunit="mu2/s" />
<Specie name="PP2BCaM" id="PP2BCaM" kdiff="0" kdiffunit="mu2/s" />
<Specie name="PP2CaMCa2" id="PP2BCaMCa2" kdiff="0"
kdiffunit="mu2/s" />
<Specie name="PP2BCaMCa4" id="PP2BCaMCa4" kdiff="0"
kdiffunit="mu2/s" />
<Specie name="CK" id="CK" kdiff="0.1" kdiffunit="mu2/s"/>
<Specie name="CKCaMCa4" id="CKCaMCa4" kdiff="0.1" kdiffunit="mu2/s"/>
<Specie name="CKpCaMCa4" id="CKpCaMCa4" kdiff="3.6" kdiffunit="mu2/s"/>
<Specie name="CKp" id="CKp" kdiff="3.6" kdiffunit="mu2/s"/>
<Specie name="CKpPP1" id="CKpPP1" kdiff="0" kdiffunit="mu2/s"/>
<Specie name="CKpCaMCa4PP1" id="CKpCaMCa4PP1" kdiff="0" kdiffunit="mu2/s"/>
<Specie name="CKpCaMCa4PP2A" id="CKpCaMCa4PP2A" kdiff="3.6" kdiffunit="mu2/s"/>
<Specie name="CKpPP2A" id="CKpPP2A" kdiff="3.6" kdiffunit="mu2/s"/>
<Specie name="PP2A" id="PP2A" kdiff="3.6" kdiffunit="mu2/s"/>
<Specie name="PP2ACKpCaMCa4" id="PP2ACKpCaMCa4" kdiff="3.6" kdiffunit="mu2/s"/>
<Specie name="PP2ACKCaMCa4" id="PP2ACKCaMCa4" kdiff="3.6" kdiffunit="mu2/s"/>
<Specie name="PP2ACKp" id="PP2ACKp" kdiff="3.6" kdiffunit="mu2/s"/>
<Specie name="pPP2A" id="pPP2A" kdiff="3.6" kdiffunit="mu2/s"/>
<Specie name="PP2ApPP2A" id="PP2ApPP2A" kdiff="3.6" kdiffunit="mu2/s"/>
<Specie name="PKA" id="PKA" kdiff="0" kdiffunit="mu2/s" />
<Specie name="PKAcAMP2" id="PKAcAMP2" kdiff="0"
kdiffunit="mu2/s" />
<Specie name="PKAcAMP4" id="PKAcAMP4" kdiff="0"
kdiffunit="mu2/s" />
<Specie name="PKAr" id="PKAr" kdiff="0" kdiffunit="mu2/s" />
<Specie name="PKAc" id="PKAc" kdiff="0.836" kdiffunit="mu2/s" />
<Specie name="I1" id="I1" kdiff="9.49" kdiffunit="mu2/s" />
<Specie name="I1PKAc" id="I1PKAc" kdiff="10.6" kdiffunit="mu2/s" />
<Specie name="Ip35" id="Ip35" kdiff="7.02" kdiffunit="mu2/s" />
<Specie name="PP1" id="PP1" kdiff="0" kdiffunit="mu2/s" />
<Specie name="Ip35PP1" id="Ip35PP1" kdiff="0" kdiffunit="mu2/s" />
<Specie name="Ip35PP2BCaMCa4" id="Ip35PP2BCaMCa4" kdiff="0"
kdiffunit="mu2/s" />
<Specie name="Ip35PP1PP2BCaMCa4" id="Ip35PP1PP2BCaMCa4" kdiff="0"
kdiffunit="mu2/s" />
<!-- AddRxns -->
<!--NMDARs-->
<Specie name="NMDAR" id="NMDAR" kdiff="0" kdiffunit="mu2/s" />
<Specie name="PKAcNMDAR" id="PKAcNMDAR" kdiff="0" kdiffunit="mu2/s" />
<Specie name="pNMDAR" id="pNMDAR" kdiff="0" kdiffunit="mu2/s" />
<Specie name="PP2BpNMDAR" id="PP2BpNMDAR" kdiff="0" kdiffunit="mu2/s" />
<Specie name="PP1pNMDAR" id="PP1pNMDAR" kdiff="0" kdiffunit="mu2/s" />
<!--AMPARs-->
<Specie name="GluR1" id="GluR1" kdiff="0" kdiffunit="mu2/s" />
<Specie name="GluR1_S845" id="GluR1_S845" kdiff="0"
kdiffunit="mu2/s" />
<Specie name="GluR1_S831" id="GluR1_S831" kdiff="0"
kdiffunit="mu2/s" />
<Specie name="GluR1_S845_S831" id="GluR1_S845_S831" kdiff="0"
kdiffunit="mu2/s" />
<Specie name="GluR1_PKAc" id="GluR1_PKAc" kdiff="0"
kdiffunit="mu2/s" />
<Specie name="GluR1_CKCaM" id="GluR1_CKCaM" kdiff="0"
kdiffunit="mu2/s" />
<Specie name="GluR1_CKpCaM" id="GluR1_CKpCaM" kdiff="0"
kdiffunit="mu2/s" />
<Specie name="GluR1_CKp" id="GluR1_CKp" kdiff="0"
kdiffunit="mu2/s" />
<Specie name="GluR1_S845_CKCaM" id="GluR1_S845_CKCaM" kdiff="0"
kdiffunit="mu2/s" />
<Specie name="GluR1_S845_CKpCaM" id="GluR1_S845_CKpCaM" kdiff="0"
kdiffunit="mu2/s" />
<Specie name="GluR1_S845_CKp" id="GluR1_S845_CKp" kdiff="0"
kdiffunit="mu2/s" />
<Specie name="GluR1_S831_PKAc" id="GluR1_S831_PKAc" kdiff="0"
kdiffunit="mu2/s" />
<Specie name="GluR1_S845_S831_PP1" id="GluR1_S845_S831_PP1"
kdiff="0" kdiffunit="mu2/s" />
<Specie name="GluR1_S845_S831_PP1_2" id="GluR1_S845_S831_PP1_2"
kdiff="0" kdiffunit="mu2/s" />
<Specie name="GluR1_S831_PP1" id="GluR1_S831_PP1" kdiff="0"
kdiffunit="mu2/s" />
<Specie name="GluR1_S845_PP1" id="GluR1_S845_PP1" kdiff="0"
kdiffunit="mu2/s" />
<Specie name="GluR1_S831_PP2A" id="GluR1_S831_PP2A" kdiff="0"
kdiffunit="mu2/s" />
<Specie name="GluR1_S845_PP2A" id="GluR1_S845_PP2A" kdiff="0"
kdiffunit="mu2/s" />
<Specie name="GluR1_S845_S831_PP2A" id="GluR1_S845_S831_PP2A"
kdiff="0" kdiffunit="mu2/s" />
<Specie name="GluR1_S845_S831_PP2A_2" id="GluR1_S845_S831_PP2A_2"
kdiff="0" kdiffunit="mu2/s" />
<Specie name="GluR1_S845_PP2B" id="GluR1_S845_PP2B" kdiff="0"
kdiffunit="mu2/s" />
<Specie name="GluR1_S845_S831_PP2B" id="GluR1_S845_S831_PP2B"
kdiff="0" kdiffunit="mu2/s" />
<Specie name="PDE4" id="PDE4" kdiff="0" kdiffunit="mu2/s" />
<Specie name="PDE4cAMP" id="PDE4cAMP" kdiff="0"
kdiffunit="mu2/s" />
<Specie name="PKAcPDE4" id="PKAcPDE4" kdiff="0"
kdiffunit="mu2/s" />
<Specie name="pPDE4" id="pPDE4" kdiff="0" kdiffunit="mu2/s" />
<Specie name="pPDE4cAMP" id="pPDE4cAMP" kdiff="0"
kdiffunit="mu2/s" />
<Specie name="PKAc_PDE4_cAMP" id="PKAc_PDE4_cAMP" kdiff="0"
kdiffunit="mu2/s" />
<!--*************************************************************************
Now, the reactions themselves SAME means params are same in both files
**************************************************************************-->
<! ICI pump out>
<Reaction name = "ICI_pump" id="ICI_pump">
<Reactant specieID="ICI"/>
<Product specieID="ICIOut"/>
<forwardRate>5e-1</forwardRate>
<reverseRate>2e-5</reverseRate>
<Q10>0.2</Q10>
</Reaction>
<!--ICI reactions 0.3 nM for ICI118,551 Functional Characterization of the βAdrenergic Receptor Subtypes Expressed by CA1 Pyramidal Cells in the Rat Hippocampus
Kristin L. Hillman, Van A. Doze1 and James E. Porter1 JPET August 2005 vol. 314 no. 2 561567 -->
<Reaction name = "ICIR_bind" id="ICIR_bind">
<Reactant specieID="ICI"/>
<Reactant specieID="bAR"/>
<Product specieID="ICIbAR"/>
<forwardRate>1.27e-5</forwardRate>
<reverseRate>3.5e-6</reverseRate>
<Q10>0.2</Q10>
</Reaction>
<!--I. *********** Ca pump and buffer rxns ************* -->
<!-- i: Ca pump high affinity - Ca + pmca <-> pmcaCa SAME-->
<Reaction name = "Ca_pump1a" id="Ca_pump1a">
<Reactant specieID="Ca"/>
<Reactant specieID="pmca"/>
<Product specieID="pmcaCa"/>
<forwardRate>0.05e-3</forwardRate>
<reverseRate>9e-3</reverseRate>
</Reaction>
<!-- ii: Ca pump high affinity - pmcaCa <-> pmca + CaOut SAME-->
<Reaction name = "Ca_pump1b" id="Ca_pump1b">
<Reactant specieID="pmcaCa"/>
<Product specieID="pmca"/>
<Product specieID="CaOut"/>
<forwardRate>3.0e-3</forwardRate>
<reverseRate>0</reverseRate>
</Reaction>
<!-- iii: Ca pump low affinity - Ca + ncx <-> ncxCa SAME-->
<Reaction name = "Ca_pump2a" id="Ca_pump2a">
<Reactant specieID="Ca"/>
<Reactant specieID="ncx"/>
<Product specieID="ncxCa"/>
<forwardRate>0.011e-3</forwardRate>
<reverseRate>11.2e-3</reverseRate>
</Reaction>
<!-- iv: Ca pump low affinity - ncxCa <-> ncx + CaOut SAME-->
<Reaction name = "Ca_pump2b" id="Ca_pump2b">
<Reactant specieID="ncxCa"/>
<Product specieID="ncx"/>
<Product specieID="CaOut"/>
<forwardRate>5.6e-3</forwardRate>
<reverseRate>0</reverseRate>
</Reaction>
<!-- v: Ca leak - CaOut + Leak <-> CaOutLeak GQ RXN FILE, Leak is sm only-->
<Reaction name = "Ca_leak" id="Ca_leak">
<Reactant specieID="CaOut"/>
<Reactant specieID="Leak"/>
<Product specieID="CaOutLeak"/>
<forwardRate>0.01e-6</forwardRate>
<reverseRate>1.1e-3</reverseRate>
</Reaction>
<!-- vi: Ca leak - CaOutLeak <-> Ca (inside) + Leak -->
<Reaction name = "Ca_leak" id="Ca_leak">
<Reactant specieID="CaOutLeak"/>
<Product specieID="Ca"/>
<Product specieID="Leak"/>
<forwardRate>1.1e-3</forwardRate>
<reverseRate>0</reverseRate>
</Reaction>
<!-- vii: Ca Buffer Ca + Calbindin <-> calbinC SAME-->
<Reaction name = "Ca_buffer" id="Ca_Buffer">
<Reactant specieID="Ca"/>
<Reactant specieID="Calbin"/>
<Product specieID="CalbinC"/>
<forwardRate>0.028e-3</forwardRate>
<reverseRate>19.6</reverseRate>
</Reaction>
<!-- Irreversible IC Ca Buffer Ca + CaB <-> CaBCa -->
<Reaction name = "CaIn_buffer" id="CaIn_buffer">
<Reactant specieID="Ca"/>
<Reactant specieID="CaB"/>
<Product specieID="CaBCa"/>
<forwardRate>2.8e-5</forwardRate>
<reverseRate>0</reverseRate>
<Q10>0.2</Q10>
</Reaction>
<Reaction name="CaCbufCa+Cbuf" id="CaCbufCa+Cbuf">
<Reactant specieID="CaCbuf" />
<Product specieID="Cbuf" />
<Product specieID="Ca" />
<forwardRate>20e-03</forwardRate>
<reverseRate>0</reverseRate>
</Reaction>
<Reaction name="CaOut+CbufNULL" id="CaOut_CbufNULL">
<Reactant specieID="CaOut" />
<Reactant specieID="Cbuf" />
<forwardRate>1e-06</forwardRate>
<reverseRate>0</reverseRate>
</Reaction>
<!--0a. Injected DaDbuf dissociates to Dbuf (external) and Da: DaDbuf -> Da + Dbuf -->
<!-- G_protein part -->
<Reaction name="ISO_buffer" id="ISO_Buffer">
<Reactant specieID="ISO" />
<Product specieID="ISOOut" />
<forwardRate>1.0e-3</forwardRate>
<reverseRate>0.0055e-6</reverseRate>
<Q10>0.2</Q10>
</Reaction>
<Reaction name="ISOIbuf--ISO+Ibuf" id="ISOIbuf--ISO+Ibuf">
<Reactant specieID="ISOIbuf" />
<Product specieID="Ibuf" />
<Product specieID="ISO" />
<forwardRate>20e-03</forwardRate>
<reverseRate>0</reverseRate>
</Reaction>
<!--0c. excess DaOut eliminated to prevent baseline shift: DaOut + Dbuf
<-> DaOutbuf -->
<Reaction name="ISOOut+Ibuf--NULL" id="ISOOut_buf--NULL">
<Reactant specieID="ISOOut" />
<Reactant specieID="Ibuf" />
<forwardRate>1e-06</forwardRate>
<reverseRate>0</reverseRate>
</Reaction>
<!-- 1) ISO + bAR
<-> ISObAR -->
<Reaction name="ISObAR_bind" id="ISObAR_bind">
<Reactant specieID="ISO" />
<Reactant specieID="bAR" />
<Product specieID="ISObAR" />
<forwardRate>0.005555e-3</forwardRate>
<!--ten times higer
than previously-->
<reverseRate>5e-3</reverseRate>
<Q10>0.2</Q10>
</Reaction>
<!-- 2) ISObAR + Gs
<-> ISObARGs -->
<Reaction name="ISObARGs_bind" id="ISObARGs_bind">
<Reactant specieID="ISObAR" />
<Reactant specieID="Gs" />
<Product specieID="ISObARGs" />
<forwardRate>0.0006e-3</forwardRate>
<reverseRate>0.001e-3</reverseRate>
<Q10>0.2</Q10>
</Reaction>
<!-- 3) Gs + bAR
<-> GsbAR -->
<Reaction name="GsbAR_bind" id="GsbAR_bind">
<Reactant specieID="Gs" />
<Reactant specieID="bAR" />
<Product specieID="GsbAR" />
<forwardRate>0.00004e-3</forwardRate>
<reverseRate>0.0003e-3</reverseRate>
<Q10>0.2</Q10>
</Reaction>
<!-- 4) GsbAR + ISO
<-> ISObARGs -->
<Reaction name="GsbARISO_bind" id="GsbARISO_bind">
<Reactant specieID="GsbAR" />
<Reactant specieID="ISO" />
<Product specieID="ISObARGs" />
<forwardRate>0.0025e-3</forwardRate>
<reverseRate>0.5e-3</reverseRate>
<Q10>0.2</Q10>
</Reaction>
<!-- 5a) ISObARGs -> ISObARGsbg + GasGTP -->
<Reaction name="GasGTP_bind" id="GasGTP_bind">
<Reactant specieID="ISObARGs" />
<Product specieID="ISObAR" />
<Product specieID="GasGTP" />
<Product specieID="Gsbg" />
<forwardRate>25e-3</forwardRate>
<reverseRate>0</reverseRate>
<Q10>0.2</Q10>
</Reaction>
<!--II. *********** Gs coupled receptor, from GS RXN FILE *************-->
<!--0a. Injected DaDbuf dissociates to Dbuf (external) and Da: DaDbuf -> Da + Dbuf -->
<Reaction name="DaDbuf--Da+Dbuf" id="DaDbuf--Da+Dbuf">
<Reactant specieID="DaDbuf" />
<Product specieID="Dbuf" />
<Product specieID="Da" />
<forwardRate>20e-03</forwardRate>
<reverseRate>0</reverseRate>
</Reaction>
<!--0b. Da or Da Agonist is extruded -->
<Reaction name="Da--DaOut" id="Da--DaOut">
<Reactant specieID="Da" />
<Product specieID="DaOut" />
<forwardRate>1.0e-3</forwardRate>
<reverseRate>0.0055e-6</reverseRate>
</Reaction>
<!--0c. excess DaOut eliminated to prevent baseline shift: DaOut + Dbuf
<-> DaOutbuf -->
<Reaction name="DaOut+Dbuf--NULL" id="DaOut_buf--NULL">
<Reactant specieID="DaOut" />
<Reactant specieID="Dbuf" />
<forwardRate>1e-06</forwardRate>
<reverseRate>0</reverseRate>
</Reaction>
<!--1a. Da + D1R
<-> DaD1R Kd=3000 source: Sunahara et al. Nature 1991-->
<Reaction name="Da+D1R--DaD1R" id="Da+D1R--DaD1R">
<Reactant specieID="Da" />
<Reactant specieID="D1R" />
<Product specieID="DaD1R" />
<forwardRate>0.00166667e-03</forwardRate>
<reverseRate>5e-03</reverseRate>
</Reaction>
<!-- 2a. DaD1R + Gsabg
<-> DaD1RGs Kd=11.666667 source: Zhuang J Neurosci 2000-->
<Reaction name="DaD1R+Gs--DaD1RGs" id="DaD1R+Gs--DaD1RGs">
<Reactant specieID="DaD1R" />
<Reactant specieID="Gs" />
<Product specieID="DaD1RGs" />
<forwardRate>0.03e-03</forwardRate>
<reverseRate>0.4e-03</reverseRate>
</Reaction>
<!-- 1b. Gsabg + D1R
<-> GsD1R Kd=35 source: PMR-->
<Reaction name="Gsabg+D1R--GsD1R" id="Gsabg+D1R--GsD1R">
<Reactant specieID="Gs" />
<Reactant specieID="D1R" />
<Product specieID="GsD1R" />
<forwardRate>0.01e-03</forwardRate>
<reverseRate>0.4e-03</reverseRate>
</Reaction>
<!-- 2b. GsD1R + Da
< - > DaD1RGs Kd=1000 source: Falkenburger J Gen Phys off-rate, Dreyer J Neurosci-->
<Reaction name="GsD1R+Da--DaD1RGs" id="GD1R+Da--DaD1RGs">
<Reactant specieID="GsD1R" />
<Reactant specieID="Da" />
<Product specieID="DaD1RGs" />
<forwardRate>0.005e-03</forwardRate>
<reverseRate>5e-03</reverseRate>
</Reaction>
<!-- 3. DaD1RGs
<-> DaD1R + GsaGTP +Gbg-->
<Reaction name="DaD1RGs-DaD1R+GsaGTP+Gbg reac"
id="DaD1RGs-DaD1R+GsaGTP+Gbg_id">
<Reactant specieID="DaD1RGs" />
<Product specieID="DaD1R" />
<Product specieID="GasGTP" />
<Product specieID="Gsbg" />
<forwardRate>0.15e-03</forwardRate>
<reverseRate>0.0</reverseRate>
</Reaction>
<!--*****************PKAc phosphorylation of DaD1R *******************************-->
<!--4. PKAc + DaD1RGs -> PKAcDaD1RGs Km= 500nM affinity for dephosphorylation is 10 lower than for cAMP accumulation for Glioma Cells, so lower the KM even more-->
<!-- AMC 06/27/12: Below are the reactions for PKA-mediated ISObAR desensitization and subsequent Gs-to-Gi switching -->
<!-- 5c1) ISObAR + PKAc -> PKAcISObAR -->
<Reaction name="ISObAR_phos" id="ISObAR_phos">
<Reactant specieID="ISObAR" />
<Reactant specieID="PKAc" />
<Product specieID="PKAcISObAR" />
<forwardRate>0.0004e-4</forwardRate>
<reverseRate>44.8e-05</reverseRate>
<Q10>0.2</Q10>
</Reaction>
<!-- 5d1) PKAcISObAR -> pISObAR + PKAc -->
<Reaction name="pISObAR_reac" id="pISObAR_reac">
<Reactant specieID="PKAcISObAR" />
<Product specieID="pISObAR" />
<Product specieID="PKAc" />
<forwardRate>1.00e-03</forwardRate>
<reverseRate>0</reverseRate>
<Q10>0.2</Q10>
</Reaction>
<!-- 5c2) pISObAR + PKAc -> PKApcISObAR -->
<Reaction name="pISObAR_phos" id="pISObAR_phos">
<Reactant specieID="pISObAR" />
<Reactant specieID="PKAc" />
<Product specieID="PKAcpISObAR" />
<forwardRate>0.006e-04</forwardRate>
<reverseRate>44.8e-04</reverseRate>
<Q10>0.2</Q10>
</Reaction>
<!-- 5d2) PKAcpISObAR -> ppISObAR + PKAc -->
<Reaction name="ppISObAR_reac" id="ppISObAR_reac">
<Reactant specieID="PKAcpISObAR" />
<Product specieID="ppISObAR" />
<Product specieID="PKAc" />
<forwardRate>1.00e-03</forwardRate>
<reverseRate>0</reverseRate>
<Q10>0.2</Q10>
</Reaction>
<!-- 5c3) ppISObAR + PKAc -> PKAcppISObAR -->
<Reaction name="ppISObAR_phos" id="ppISObAR_phos">
<Reactant specieID="ppISObAR" />
<Reactant specieID="PKAc" />
<Product specieID="PKAcppISObAR" />
<forwardRate>0.034e-03</forwardRate>
<reverseRate>44.8e-04</reverseRate>
<Q10>0.2</Q10>
</Reaction>
<!-- 5d3) PKAcppISObAR -> pppISObAR + PKAc -->
<Reaction name="pppISObAR_reac" id="pppISObAR_reac">
<Reactant specieID="PKAcppISObAR" />
<Product specieID="pppISObAR" />
<Product specieID="PKAc" />
<forwardRate>1.00e-03</forwardRate>
<reverseRate>0</reverseRate>
<Q10>0.2</Q10>
</Reaction>
<!-- 5c4) pppISObAR + PKAc -> PKAcpppISObAR -->
<Reaction name="pppISObAR_phos" id="pppISObAR_phos">
<Reactant specieID="pppISObAR" />
<Reactant specieID="PKAc" />
<Product specieID="PKAcpppISObAR" />
<forwardRate>13.4e-03</forwardRate>
<reverseRate>44.8e-04</reverseRate>
<Q10>0.2</Q10>
</Reaction>
<!-- 5d4) PKAcpppISObAR -> ppppISObAR + PKAc -->
<Reaction name="ppppISObAR_reac" id="ppppISObAR_reac">
<Reactant specieID="PKAcpppISObAR" />
<Product specieID="ppppISObAR" />
<Product specieID="PKAc" />
<forwardRate>1.00e-03</forwardRate>
<reverseRate>0</reverseRate>
<Q10>0.2</Q10>
</Reaction>
<!-- 5e) ppppISObAR + Gi -> ppppISObARGi -->
<Reaction name="ppppISObARGi_bind" id="ppppISObARGi_bind">
<Reactant specieID="ppppISObAR" />
<Reactant specieID="Gi" />
<Product specieID="ppppISObARGi" />
<forwardRate>0.15e-2</forwardRate>
<reverseRate>0.25e-2</reverseRate>
<Q10>0.2</Q10>
</Reaction>
<!-- 5f) ppppISObARGi -> ppppISObARGibg + GaiGTP -->
<Reaction name="GaiGTP_bind" id="GaiGTP_bind">
<Reactant specieID="ppppISObARGi" />
<Product specieID="Gibg" />
<Product specieID="ppppISObAR" />
<Product specieID="GaiGTP" />
<forwardRate>0.2e-3</forwardRate>
<reverseRate>0</reverseRate>
<Q10>0.2</Q10>
</Reaction>
<!-- 5h1) pISObAR -> ISObAR -->
<Reaction name="pISObAR_dephos" id="pISObAR_dephos">
<Reactant specieID="pISObAR" />
<Product specieID="ISObAR" />
<forwardRate>0.25e-05</forwardRate>
<reverseRate>0</reverseRate>
<Q10>0.2</Q10>
</Reaction>
<!-- 5h2) ppISObAR -> pISObAR -->
<Reaction name="ppISObAR_dephos" id="ppISObAR_dephos">
<Reactant specieID="ppISObAR" />
<Product specieID="pISObAR" />
<forwardRate>0.25e-05</forwardRate>
<reverseRate>0</reverseRate>
<Q10>0.2</Q10>
</Reaction>
<!-- 5h3) pppISObAR -> ppISObAR -->
<Reaction name="pppISObAR_dephos" id="pppISObAR_dephos">
<Reactant specieID="pppISObAR" />
<Product specieID="ppISObAR" />
<forwardRate>0.25e-05</forwardRate>
<reverseRate>0</reverseRate>
<Q10>0.2</Q10>
</Reaction>
<!-- 5h3) ppppISObAR -> pppISObAR -->
<Reaction name="ppppISObAR_dephos" id="ppppISObAR_dephos">
<Reactant specieID="ppppISObAR" />
<Product specieID="pppISObAR" />
<forwardRate>0.25e-05</forwardRate>
<reverseRate>0</reverseRate>
<Q10>0.2</Q10>
</Reaction>
<!-- 5ac1) bAR + PKAc -> PKAcbAR -->
<Reaction name="bAR_phos" id="bAR_phos">
<Reactant specieID="bAR" />
<Reactant specieID="PKAc" />
<Product specieID="PKAcbAR" />
<forwardRate>0.0004e-05</forwardRate>
<reverseRate>44.8e-05</reverseRate>
<Q10>0.2</Q10>
</Reaction>
<!-- 5ad1) PKAcbAR -> pbAR + PKAc -->
<Reaction name="pbAR_reac" id="pbAR_reac">
<Reactant specieID="PKAcbAR" />
<Product specieID="pbAR" />
<Product specieID="PKAc" />
<forwardRate>1.00e-03</forwardRate>
<reverseRate>0</reverseRate>
<Q10>0.2</Q10>
</Reaction>
<!-- 5ac2) pbAR + PKAc -> PKAcpbAR -->
<Reaction name="pbAR_phos" id="pbAR_phos">
<Reactant specieID="pbAR" />
<Reactant specieID="PKAc" />
<Product specieID="PKAcpbAR" />
<forwardRate>0.004e-04</forwardRate>
<reverseRate>44.8e-04</reverseRate>
<Q10>0.2</Q10>
</Reaction>
<!-- 5d2) PKAcpbAR -> ppbAR + PKAc -->
<Reaction name="ppbAR_reac" id="ppbAR_reac">
<Reactant specieID="PKAcpbAR" />
<Product specieID="ppbAR" />
<Product specieID="PKAc" />
<forwardRate>1.00e-03</forwardRate>
<reverseRate>0</reverseRate>
<Q10>0.2</Q10>
</Reaction>
<!-- 5ac3) ppbAR + PKAc -> PKAcppbAR -->
<Reaction name="ppbAR_phos" id="ppbAR_phos">
<Reactant specieID="ppbAR" />
<Reactant specieID="PKAc" />
<Product specieID="PKAcppbAR" />
<forwardRate>0.016e-03</forwardRate>
<reverseRate>44.8e-04</reverseRate>
<Q10>0.2</Q10>
</Reaction>
<!-- 5ad3) PKAcppbAR -> pppbAR + PKAc -->
<Reaction name="pppbAR_reac" id="pppbAR_reac">
<Reactant specieID="PKAcppbAR" />
<Product specieID="pppbAR" />
<Product specieID="PKAc" />
<forwardRate>1.00e-03</forwardRate>
<reverseRate>0</reverseRate>
<Q10>0.2</Q10>
</Reaction>
<!-- 5ac4) pppbAR + PKAc -> PKAcpppbAR -->
<Reaction name="pppbAR_phos" id="pppbAR_phos">
<Reactant specieID="pppbAR" />
<Reactant specieID="PKAc" />
<Product specieID="PKAcpppbAR" />
<forwardRate>1.6e-03</forwardRate>
<reverseRate>44.8e-04</reverseRate>
<Q10>0.2</Q10>
</Reaction>
<!-- 5ad4) PKAcpppISObAR -> ppppISObAR + PKAc -->
<Reaction name="ppppbAR_reac" id="ppppbAR_reac">
<Reactant specieID="PKAcpppbAR" />
<Product specieID="ppppbAR" />
<Product specieID="PKAc" />
<forwardRate>1.00e-03</forwardRate>
<reverseRate>0</reverseRate>
<Q10>0.2</Q10>
</Reaction>
<!-- 5ae) ppppbAR + Gi -> ppppbARGi -->
<Reaction name="ppppbARGi_bind" id="ppppbARGi_bind">
<Reactant specieID="ppppbAR" />
<Reactant specieID="Gi" />
<Product specieID="ppppbARGi" />
<forwardRate>0.75e-3</forwardRate>
<reverseRate>0.125e-2</reverseRate>
<Q10>0.2</Q10>
</Reaction>
<!-- 5af) ppppbARGi -> ppppbARGibg + GaiGTP -->
<Reaction name="GaiGTP_bind_r" id="GaiGTP_bind_r">
<Reactant specieID="ppppbARGi" />
<Product specieID="ppppbAR" />
<Product specieID="Gibg" />
<Product specieID="GaiGTP" />
<forwardRate>0.1e-3</forwardRate>
<reverseRate>0</reverseRate>
<Q10>0.2</Q10>
</Reaction>
<!-- 5ah1) pISObAR -> ISObAR -->
<Reaction name="pbAR_dephos" id="pbAR_dephos">
<Reactant specieID="pbAR" />
<Product specieID="bAR" />
<forwardRate>0.25e-05</forwardRate>
<reverseRate>0</reverseRate>
<Q10>0.2</Q10>
</Reaction>
<!-- 5ah2) ppISObAR -> pISObAR -->
<Reaction name="ppbAR_dephos" id="ppbAR_dephos">
<Reactant specieID="ppbAR" />
<Product specieID="pbAR" />
<forwardRate>0.25e-05</forwardRate>
<reverseRate>0</reverseRate>
<Q10>0.2</Q10>
</Reaction>
<!-- 5ah3) pppbAR -> ppbAR -->
<Reaction name="pppbAR_dephos" id="pppbAR_dephos">
<Reactant specieID="pppbAR" />
<Product specieID="ppbAR" />
<forwardRate>0.25e-05</forwardRate>
<reverseRate>0</reverseRate>
<Q10>0.2</Q10>
</Reaction>
<!-- 5ah3) ppppbAR -> pppbAR -->
<Reaction name="ppppbAR_dephos" id="ppppbAR_dephos">
<Reactant specieID="ppppbAR" />
<Product specieID="pppbAR" />
<forwardRate>0.25e-05</forwardRate>
<reverseRate>0</reverseRate>
<Q10>0.2</Q10>
</Reaction>
<!-- 6a) GasGTP -> GasGDP -->
<Reaction name="GasGTP_disso" id="GasGTP_disso">
<Reactant specieID="GasGTP" />
<Product specieID="GasGDP" />
<forwardRate>10e-3</forwardRate>
<reverseRate>0</reverseRate>
<Q10>0.2</Q10>
</Reaction>
<!-- 7a) GasGDP + Gbg -> Gs -->
<Reaction name="Gs_form" id="Gs_form">
<Reactant specieID="GasGDP" />
<Reactant specieID="Gsbg" />
<Product specieID="Gs" />
<forwardRate>100e-3</forwardRate>
<reverseRate>0</reverseRate>
<Q10>0.2</Q10>
</Reaction>
<!-- 6b) GaiGTP -> GaiGDP -->
<Reaction name="GaiGTP_disso" id="GaiGTP_disso">
<Reactant specieID="GaiGTP" />
<Product specieID="GaiGDP" />
<forwardRate>0.125e-3</forwardRate>
<reverseRate>0</reverseRate>
<Q10>0.2</Q10>
</Reaction>
<!-- 7b) GaiGDP + Gbg -> Gi -->
<Reaction name="Gi_form" id="Gi_form">
<Reactant specieID="GaiGDP" />
<Reactant specieID="Gibg" />
<Product specieID="Gi" />
<forwardRate>1.25e-3</forwardRate>
<reverseRate>0</reverseRate>
<Q10>0.2</Q10>
</Reaction>
<!--Fine, but I in future would like to compare above to Linderman who studies G proteins. Above is old from Destexhe-->
<!-- AC part:AC1 & AC8 activation through CaMCa4 -->
<!-- Add 1 more reaction(11) for AC1 bound CaMCa4 form binds Gsa-->
<!-- reaction 4, kcat adjusted by 4X faster than previous due to facilitate-->
<!-- 1a) GasGTP + AC1
<-> E(AC1GasGTP) -->
<Reaction name="GasGTP_AC1_reac" id="GasGTP_AC1_reac">
<Reactant specieID="GasGTP" />
<Reactant specieID="AC1" />
<Product specieID="AC1GasGTP" />
<forwardRate>0.0385e-3</forwardRate>
<reverseRate>10e-3</reverseRate>
<Q10>0.2</Q10>
</Reaction>
<!-- 1b) E + CaMCa4
<-> ECaM(AC1GasGTPCaMCa4) -->
<Reaction name="AC1GasGTP_CaMCa4_Reac" id="AC1GasGTP_CaMCa4_Reac">
<Reactant specieID="AC1GasGTP" />
<Reactant specieID="CaMCa4" />
<Product specieID="AC1GasGTPCaMCa4" />
<forwardRate>6.0e-6</forwardRate>
<reverseRate>0.9e-3</reverseRate>
<Q10>0.2</Q10>
</Reaction>
<!-- 1c) ECaM + ATP
<-> ECaMATP -->
<Reaction name="AC1GasGTPCaMCa4_ATP_Reac"
id="AC1GasGTPCaMCa4_ATP_Reac">
<Reactant specieID="AC1GasGTPCaMCa4" />
<Reactant specieID="ATP" />
<Product specieID="AC1GasGTPCaMCa4ATP" />
<forwardRate>0.01e-3</forwardRate>
<reverseRate>2273e-3</reverseRate>
<Q10>0.2</Q10>
</Reaction>
<!-- 1d) ECaMATP -> ECaM + cAMP -->
<Reaction name="AC1GasGTPCaMCa4ATP_diss"
id="AC1GasGTPCaMCa4ATP_diss">
<Reactant specieID="AC1GasGTPCaMCa4ATP" />
<Product specieID="cAMP" />
<Product specieID="AC1GasGTPCaMCa4" />
<forwardRate>0.02842</forwardRate>
<reverseRate>0</reverseRate>
<Q10>0.2</Q10>
</Reaction>
<!-- 2a) GaiGTP + E
<-> F(AC1GasGTPGaiGTP) -->
<Reaction name="GaiGTP_AC1GasGTP_reac" id="GaiGTP_AC1GasGTP_reac">
<Reactant specieID="GaiGTP" />
<Reactant specieID="AC1GasGTP" />
<Product specieID="AC1GasGTPGaiGTP" />
<forwardRate>0.0625e-3</forwardRate>
<reverseRate>10e-3</reverseRate>
<Q10>0.2</Q10>
</Reaction>
<!-- 2b) F + CaMCa4
<-> FCaM(AC1GasGTPGaiGTPCaMCa4) -->
<Reaction name="AC1GasGTPGaiGTP_CaMCa4_Reac"
id="AC1GasGTPGaiGTP_CaMCa4_Reac">
<Reactant specieID="AC1GasGTPGaiGTP" />
<Reactant specieID="CaMCa4" />
<Product specieID="AC1GasGTPGaiGTPCaMCa4" />
<forwardRate>0.006e-3</forwardRate>
<reverseRate>0.9e-3</reverseRate>
<Q10>0.2</Q10>
</Reaction>
<!-- 2c) FCaM + ATP
<-> FCaMATP -->
<Reaction name="AC1GasGTPGaiGTPCaMCa4_ATP_Reac"
id="AC1GasGTPGaiGTPCaMCa4_ATP_Reac">
<Reactant specieID="AC1GasGTPGaiGTPCaMCa4" />
<Reactant specieID="ATP" />
<Product specieID="AC1GsGiCaMCa4ATP" />
<forwardRate>0.01e-3</forwardRate>
<reverseRate>2273e-3</reverseRate>
<Q10>0.2</Q10>
</Reaction>
<!-- 2d) FCaMATP -> FCaM + cAMP -->
<Reaction name="AC1GsGiCaMCa4ATP_diss" id="AC1GsGiCaMCa4ATP_diss">
<Reactant specieID="AC1GsGiCaMCa4ATP" />
<Product specieID="cAMP" />
<Product specieID="AC1GasGTPGaiGTPCaMCa4" />
<forwardRate>2.842e-3</forwardRate>
<reverseRate>0</reverseRate>
<Q10>0.2</Q10>
</Reaction>
<!-- 3a) GaiGTP + AC1CaM4
<-> GCaM(AC1CaMCa4GaiGTP) -->
<Reaction name="GaiGTP_AC1CaMCa4_reac" id="GaiGTP_AC1CaMCa4_reac">
<Reactant specieID="GaiGTP" />
<Reactant specieID="AC1CaMCa4" />
<Product specieID="AC1GaiGTPCaMCa4" />
<forwardRate>0.0625e-3</forwardRate>
<reverseRate>10e-3</reverseRate>
<Q10>0.2</Q10>
</Reaction>
<!-- 3b) G + CaMCa4
<-> GCaM(AC1GaiGTPCaMCa4) -->
<!-- 3c) GCaM + ATP
<-> GCaMATP -->
<Reaction name="AC1GaiGTPCaMCa4_ATP_Reac"
id="AC1GaiGTPCaMCa4_ATP_Reac">
<Reactant specieID="AC1GaiGTPCaMCa4" />
<Reactant specieID="ATP" />
<Product specieID="AC1GaiGTPCaMCa4ATP" />
<forwardRate>0.01e-3</forwardRate>
<reverseRate>2273e-3</reverseRate>
<Q10>0.2</Q10>
</Reaction>
<!-- 3d) GCaMATP -> GCaM + cAMP -->
<Reaction name="AC1GaiGTPCaMCa4ATP_diss"
id="AC1GaiGTPCaMCa4ATP_diss">
<Reactant specieID="AC1GaiGTPCaMCa4ATP" />
<Product specieID="cAMP" />
<Product specieID="AC1GaiGTPCaMCa4" />
<forwardRate>0.5684e-3</forwardRate>
<reverseRate>0</reverseRate>
<Q10>0.2</Q10>
</Reaction>
<!-- 5) AC1 + CaMCa4
<-> AC1CaM -->
<Reaction name="AC1CaMCa4_Reac" id="AC1CaMCa4_Reac">
<Reactant specieID="AC1" />
<Reactant specieID="CaMCa4" />
<Product specieID="AC1CaMCa4" />
<forwardRate>0.006e-3</forwardRate>
<reverseRate>0.9e-3</reverseRate>
<Q10>0.2</Q10>
</Reaction>
<!-- 6) AC1CaM + ATP
<-> AC1CaMATP -->
<Reaction name="AC1CaMCa4ATP_bind" id="AC1CaMCa4ATP_bind">
<Reactant specieID="AC1CaMCa4" />
<Reactant specieID="ATP" />
<Product specieID="AC1CaMCa4ATP" />
<forwardRate>0.01e-3</forwardRate>
<reverseRate>2273e-3</reverseRate>
<Q10>0.2</Q10>
</Reaction>
<!-- 7) AC1CaMATP -> AC1CaM +cAMP -->
<Reaction name="AC1CaMCa4ATP_disso" id="AC1CaMCa4ATP_disso">
<Reactant specieID="AC1CaMCa4ATP" />
<Product specieID="cAMP" />
<Product specieID="AC1CaMCa4" />
<forwardRate>5.684e-3</forwardRate>
<reverseRate>0</reverseRate>
<Q10>0.2</Q10>
</Reaction>
<!-- 6a) G + GasGTP
<-> F(AC1GasGTPGaiGTP) -->
<Reaction name="AC1GaiGTP_GasGTP_Reac" id="AC1GaiGTP_GasGTP_Reac">
<Reactant specieID="AC1GaiGTP" />
<Reactant specieID="GasGTP" />
<Product specieID="AC1GasGTPGaiGTP" />
<forwardRate>0.0000625</forwardRate>
<reverseRate>10e-3</reverseRate>
<Q10>0.2</Q10>
</Reaction>
<!--AC1 is fine - thermodynamically balanced. But what is source for affinity to ATP? 2273 is very large backward rate;large Kb prevents large E-S intermidiate complex, Kd(cAMP)=2.3(+-)0.8 mM, low affinity -->
<!-- 8) AC8 + CaMCa4
<-> AC8CaM -->
<Reaction name="AC8_CaMCa4_bind" id="AC8_CaMCa4_bind">
<Reactant specieID="AC8" />
<Reactant specieID="CaMCa4" />
<Product specieID="AC8CaMCa4" />
<forwardRate>0.00125e-3</forwardRate>
<reverseRate>1e-3</reverseRate>
<Q10>0.2</Q10>
</Reaction>
<!-- 9) AC8CaM + ATP
<-> AC8CaMATP -->
<Reaction name="AC8CaMCa4_ATP_bind" id="AC8CaMCa4_ATP_bind">
<Reactant specieID="AC8CaMCa4" />
<Reactant specieID="ATP" />
<Product specieID="AC8CaMCa4ATP" />
<forwardRate>0.01e-3</forwardRate>
<reverseRate>2273e-3</reverseRate>
<Q10>0.2</Q10>
</Reaction>
<!-- 10) AC8CaMATP -> AC8CaM + cAMP -->
<Reaction name="AC8CaMCa4ATP_disso" id="AC8CaMCa4ATP_disso">
<Reactant specieID="AC8CaMCa4ATP" />
<Product specieID="cAMP" />
<Product specieID="AC8CaMCa4" />
<forwardRate>2.842e-3</forwardRate>
<reverseRate>0</reverseRate>
<Q10>0.2</Q10>
</Reaction>
<!-- CaM, CaMCa4 and PP2B Binding -->
<!--1) CaM + 2Ca
<-> CaMCa2 -->
<!--This is the C site-->
<Reaction name="CaM_bind" id="CaM_bind">
<Reactant specieID="CaM" />
<Reactant specieID="Ca" n="2" />
<Product specieID="CaMCa2" />