diff --git a/frontend/src/component/segments/CreateSegmentButton/CreateSegmentButton.tsx b/frontend/src/component/segments/CreateSegmentButton/CreateSegmentButton.tsx
index 7ca62fe951bb..74711b6a7880 100644
--- a/frontend/src/component/segments/CreateSegmentButton/CreateSegmentButton.tsx
+++ b/frontend/src/component/segments/CreateSegmentButton/CreateSegmentButton.tsx
@@ -6,8 +6,12 @@ import PermissionButton from 'component/common/PermissionButton/PermissionButton
 import { NAVIGATE_TO_CREATE_SEGMENT } from 'utils/testIds';
 import { useNavigate } from 'react-router-dom';
 import { useOptionalPathParam } from 'hooks/useOptionalPathParam';
+import type { FC } from 'react';
 
-export const CreateSegmentButton = () => {
+export const CreateSegmentButton: FC<{
+    disabled: boolean;
+    tooltip?: string;
+}> = ({ disabled, tooltip }) => {
     const projectId = useOptionalPathParam('projectId');
     const navigate = useNavigate();
 
@@ -22,6 +26,10 @@ export const CreateSegmentButton = () => {
             }}
             permission={[CREATE_SEGMENT, UPDATE_PROJECT_SEGMENT]}
             projectId={projectId}
+            disabled={disabled}
+            tooltipProps={{
+                title: tooltip,
+            }}
             data-testid={NAVIGATE_TO_CREATE_SEGMENT}
         >
             New segment
diff --git a/frontend/src/component/segments/SegmentTable/SegmentTable.test.tsx b/frontend/src/component/segments/SegmentTable/SegmentTable.test.tsx
index 8c4d4ba65be6..87b66b05d8a2 100644
--- a/frontend/src/component/segments/SegmentTable/SegmentTable.test.tsx
+++ b/frontend/src/component/segments/SegmentTable/SegmentTable.test.tsx
@@ -2,6 +2,7 @@ import { render } from 'utils/testRenderer';
 import { screen } from '@testing-library/react';
 import { SegmentTable } from './SegmentTable';
 import { testServerRoute, testServerSetup } from 'utils/testServer';
+import { CREATE_SEGMENT } from '../../providers/AccessProvider/permissions';
 
 const server = testServerSetup();
 
@@ -23,6 +24,10 @@ const setupRoutes = () => {
     testServerRoute(server, '/api/admin/ui-config', {
         flags: {
             SE: true,
+            resourceLimits: true,
+        },
+        resourceLimits: {
+            segments: 2,
         },
     });
 };
@@ -30,8 +35,14 @@ const setupRoutes = () => {
 test('should show the count of projects and features used in', async () => {
     setupRoutes();
 
-    render(<SegmentTable />);
+    render(<SegmentTable />, { permissions: [{ permission: CREATE_SEGMENT }] });
+
+    const loadingSegment = await screen.findByText('New segment');
+    expect(loadingSegment).toBeDisabled();
 
     await screen.findByText('2 feature flags');
     await screen.findByText('3 projects');
+
+    const segment = await screen.findByText('New segment');
+    expect(segment).not.toBeDisabled();
 });
diff --git a/frontend/src/component/segments/SegmentTable/SegmentTable.tsx b/frontend/src/component/segments/SegmentTable/SegmentTable.tsx
index c0d33139688e..c10d98446394 100644
--- a/frontend/src/component/segments/SegmentTable/SegmentTable.tsx
+++ b/frontend/src/component/segments/SegmentTable/SegmentTable.tsx
@@ -29,15 +29,41 @@ import { useConditionallyHiddenColumns } from 'hooks/useConditionallyHiddenColum
 import { TextCell } from 'component/common/Table/cells/TextCell/TextCell';
 import { useOptionalPathParam } from 'hooks/useOptionalPathParam';
 import { UsedInCell } from 'component/context/ContextList/UsedInCell';
+import useUiConfig from 'hooks/api/getters/useUiConfig/useUiConfig';
+import { useUiFlag } from 'hooks/useUiFlag';
+
+const useSegmentLimit = (segmentsLimit: number, segmentsCount: number) => {
+    const resourceLimitsEnabled = useUiFlag('resourceLimits');
+    const limitReached =
+        resourceLimitsEnabled && segmentsCount >= segmentsLimit;
+
+    return {
+        limitReached,
+        limitMessage: limitReached
+            ? `Limit of ${segmentsCount} segments reached`
+            : undefined,
+    };
+};
 
 export const SegmentTable = () => {
     const projectId = useOptionalPathParam('projectId');
-    const { segments, loading } = useSegments();
+    const { segments, loading: loadingSegments } = useSegments();
     const isSmallScreen = useMediaQuery(theme.breakpoints.down('md'));
     const [initialState] = useState({
         sortBy: [{ id: 'createdAt' }],
         hiddenColumns: ['description'],
     });
+    const { uiConfig, loading: loadingConfig } = useUiConfig();
+    const segmentLimit = uiConfig.resourceLimits.segments;
+    const segmentCount = segments?.length || 0;
+
+    const { limitReached, limitMessage } = useSegmentLimit(
+        segmentLimit,
+        segmentCount,
+    );
+
+    const createSegmentDisabled =
+        loadingSegments || loadingConfig || limitReached;
 
     const data = useMemo(() => {
         if (!segments) {
@@ -112,15 +138,18 @@ export const SegmentTable = () => {
                                 onChange={setGlobalFilter}
                             />
                             <PageHeader.Divider />
-                            <CreateSegmentButton />
+                            <CreateSegmentButton
+                                disabled={createSegmentDisabled}
+                                tooltip={limitMessage}
+                            />
                         </>
                     }
                 />
             }
-            isLoading={loading}
+            isLoading={loadingSegments}
         >
             <ConditionallyRender
-                condition={!loading && data.length === 0}
+                condition={!loadingSegments && data.length === 0}
                 show={
                     <TablePlaceholder>
                         <SegmentEmpty />
diff --git a/frontend/src/hooks/api/getters/useUiConfig/defaultValue.tsx b/frontend/src/hooks/api/getters/useUiConfig/defaultValue.tsx
index 033faefd9149..b81dcbd045e8 100644
--- a/frontend/src/hooks/api/getters/useUiConfig/defaultValue.tsx
+++ b/frontend/src/hooks/api/getters/useUiConfig/defaultValue.tsx
@@ -41,5 +41,6 @@ export const defaultValue: IUiConfig = {
         environments: 50,
         constraintValues: 250,
         projects: 500,
+        segments: 300,
     },
 };
diff --git a/frontend/src/openapi/models/resourceLimitsSchema.ts b/frontend/src/openapi/models/resourceLimitsSchema.ts
index f04845a529a4..5a5770852def 100644
--- a/frontend/src/openapi/models/resourceLimitsSchema.ts
+++ b/frontend/src/openapi/models/resourceLimitsSchema.ts
@@ -32,4 +32,6 @@ export interface ResourceLimitsSchema {
     constraintValues: number;
     /** The maximum number of projects allowed. */
     projects: number;
+    /** The maximum number of segment allowed. */
+    segments: number;
 }