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Copy path10. Mark duplicates, duplicates will not be removed but will be marked for quality checks
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10. Mark duplicates, duplicates will not be removed but will be marked for quality checks
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#!/bin/bash
#SBATCH -n 8
#SBATCH --job-name=markdups
#SBATCH -t 2-0:0
#SBATCH -A mwilsons
#SBATCH -o slurm.markdups.out
#SBATCH -e slurm.markdups.err
#SBATCH --mail-type=END,FAIL
#SBATCH [email protected]
#--------------------------
#--------------------------
# 1. start timer
#--------------------------
date
#--------------------------
# 2. move to directory and load programs
#--------------------------
cd /home/kcolney/cancer_HaynesLab/
module load bamtools/2.4.0
module load java/latest
#--------------------------
# 3. BAMtools sort, mark duplicates
#--------------------------
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH1_S1_L001.sort.bam OUTPUT=KH1_S1_L001.sort.markdup.bam METRICS_FILE=KH1_S1_L001sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH1_S1_L002.sort.bam OUTPUT=KH1_S1_L002.sort.markdup.bam METRICS_FILE=KH1_S1_L002sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH1_S1_L003.sort.bam OUTPUT=KH1_S1_L003.sort.markdup.bam METRICS_FILE=KH1_S1_L003sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH1_S1_L004.sort.bam OUTPUT=KH1_S1_L004.sort.markdup.bam METRICS_FILE=KH1_S1_L004sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH2_S2_L001.sort.bam OUTPUT=KH2_S2_L001.sort.markdup.bam METRICS_FILE=KH2_S2_L001sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH2_S2_L002.sort.bam OUTPUT=KH2_S2_L002.sort.markdup.bam METRICS_FILE=KH2_S2_L002sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH2_S2_L003.sort.bam OUTPUT=KH2_S2_L003.sort.markdup.bam METRICS_FILE=KH2_S2_L003sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH2_S2_L004.sort.bam OUTPUT=KH2_S2_L004.sort.markdup.bam METRICS_FILE=KH2_S2_L004sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH3_S3_L001.sort.bam OUTPUT=KH3_S3_L001.sort.markdup.bam METRICS_FILE=KH3_S3_L001sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH3_S3_L002.sort.bam OUTPUT=KH3_S3_L002.sort.markdup.bam METRICS_FILE=KH3_S3_L002sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH3_S3_L003.sort.bam OUTPUT=KH3_S3_L003.sort.markdup.bam METRICS_FILE=KH3_S3_L003sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH3_S3_L004.sort.bam OUTPUT=KH3_S3_L004.sort.markdup.bam METRICS_FILE=KH3_S3_L004sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH4_S4_L001.sort.bam OUTPUT=KH4_S4_L001.sort.markdup.bam METRICS_FILE=KH4_S4_L001sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH4_S4_L002.sort.bam OUTPUT=KH4_S4_L002.sort.markdup.bam METRICS_FILE=KH4_S4_L002sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH4_S4_L003.sort.bam OUTPUT=KH4_S4_L003.sort.markdup.bam METRICS_FILE=KH4_S4_L003sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH4_S4_L004.sort.bam OUTPUT=KH4_S4_L004.sort.markdup.bam METRICS_FILE=KH4_S4_L004sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH5_S5_L001.sort.bam OUTPUT=KH5_S5_L001.sort.markdup.bam METRICS_FILE=KH5_S5_L001sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH5_S5_L002.sort.bam OUTPUT=KH5_S5_L002.sort.markdup.bam METRICS_FILE=KH5_S5_L002sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH5_S5_L003.sort.bam OUTPUT=KH5_S5_L003.sort.markdup.bam METRICS_FILE=KH5_S5_L003sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH5_S5_L004.sort.bam OUTPUT=KH5_S5_L004.sort.markdup.bam METRICS_FILE=KH5_S5_L004sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH6_S6_L001.sort.bam OUTPUT=KH6_S6_L001.sort.markdup.bam METRICS_FILE=KH6_S6_L001sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH6_S6_L002.sort.bam OUTPUT=KH6_S6_L002.sort.markdup.bam METRICS_FILE=KH6_S6_L002sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH6_S6_L003.sort.bam OUTPUT=KH6_S6_L003.sort.markdup.bam METRICS_FILE=KH6_S6_L003sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH6_S6_L004.sort.bam OUTPUT=KH6_S6_L004.sort.markdup.bam METRICS_FILE=KH6_S6_L004sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH7_S7_L001.sort.bam OUTPUT=KH7_S7_L001.sort.markdup.bam METRICS_FILE=KH7_S7_L001sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH7_S7_L002.sort.bam OUTPUT=KH7_S7_L002.sort.markdup.bam METRICS_FILE=KH7_S7_L002sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH7_S7_L003.sort.bam OUTPUT=KH7_S7_L003.sort.markdup.bam METRICS_FILE=KH7_S7_L003sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH7_S7_L004.sort.bam OUTPUT=KH7_S7_L004.sort.markdup.bam METRICS_FILE=KH7_S7_L004sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH8_S8_L001.sort.bam OUTPUT=KH8_S8_L001.sort.markdup.bam METRICS_FILE=KH8_S8_L001sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH8_S8_L002.sort.bam OUTPUT=KH8_S8_L002.sort.markdup.bam METRICS_FILE=KH8_S8_L002sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH8_S8_L003.sort.bam OUTPUT=KH8_S8_L003.sort.markdup.bam METRICS_FILE=KH8_S8_L003sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH8_S8_L004.sort.bam OUTPUT=KH8_S8_L004.sort.markdup.bam METRICS_FILE=KH8_S8_L004sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH9_S1_L001.sort.bam OUTPUT=KH9_S1_L001.sort.markdup.bam METRICS_FILE=KH9_S1_L001sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH9_S1_L002.sort.bam OUTPUT=KH9_S1_L002.sort.markdup.bam METRICS_FILE=KH9_S1_L002sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH9_S1_L003.sort.bam OUTPUT=KH9_S1_L003.sort.markdup.bam METRICS_FILE=KH9_S1_L003sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH9_S1_L004.sort.bam OUTPUT=KH9_S1_L004.sort.markdup.bam METRICS_FILE=KH9_S1_L004sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH10_S2_L001.sort.bam OUTPUT=KH10_S2_L001.sort.markdup.bam METRICS_FILE=KH10_S2_L001sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH10_S2_L004.sort.bam OUTPUT=KH10_S2_L004.sort.markdup.bam METRICS_FILE=KH10_S2_L004sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH10_S2_L002.sort.bam OUTPUT=KH10_S2_L002.sort.markdup.bam METRICS_FILE=KH10_S2_L002sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH10_S2_L003.sort.bam OUTPUT=KH10_S2_L003.sort.markdup.bam METRICS_FILE=KH10_S2_L003sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH11_S3_L001.sort.bam OUTPUT=KH11_S3_L001.sort.markdup.bam METRICS_FILE=KH11_S3_L001sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH11_S3_L002.sort.bam OUTPUT=KH11_S3_L002.sort.markdup.bam METRICS_FILE=KH11_S3_L002sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH11_S3_L003.sort.bam OUTPUT=KH11_S3_L003.sort.markdup.bam METRICS_FILE=KH11_S3_L003sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH11_S3_L004.sort.bam OUTPUT=KH11_S3_L004.sort.markdup.bam METRICS_FILE=KH11_S3_L004sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH12_S4_L001.sort.bam OUTPUT=KH12_S4_L001.sort.markdup.bam METRICS_FILE=KH12_S4_L001sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH12_S4_L002.sort.bam OUTPUT=KH12_S4_L002.sort.markdup.bam METRICS_FILE=KH12_S4_L002sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH12_S4_L003.sort.bam OUTPUT=KH12_S4_L003.sort.markdup.bam METRICS_FILE=KH12_S4_L003sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH12_S4_L004.sort.bam OUTPUT=KH12_S4_L004.sort.markdup.bam METRICS_FILE=KH12_S4_L004sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH13_S5_L001.sort.bam OUTPUT=KH13_S5_L001.sort.markdup.bam METRICS_FILE=KH13_S5_L001sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH13_S5_L002.sort.bam OUTPUT=KH13_S5_L002.sort.markdup.bam METRICS_FILE=KH13_S5_L002sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH13_S5_L003.sort.bam OUTPUT=KH13_S5_L003.sort.markdup.bam METRICS_FILE=KH13_S5_L003sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH13_S5_L004.sort.bam OUTPUT=KH13_S5_L004.sort.markdup.bam METRICS_FILE=KH13_S5_L004sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH14_S6_L001.sort.bam OUTPUT=KH14_S6_L001.sort.markdup.bam METRICS_FILE=KH14_S6_L001sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH14_S6_L002.sort.bam OUTPUT=KH14_S6_L002.sort.markdup.bam METRICS_FILE=KH14_S6_L002sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH14_S6_L003.sort.bam OUTPUT=KH14_S6_L003.sort.markdup.bam METRICS_FILE=KH14_S6_L003sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH14_S6_L004.sort.bam OUTPUT=KH14_S6_L004.sort.markdup.bam METRICS_FILE=KH14_S6_L004sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH15_S7_L001.sort.bam OUTPUT=KH15_S7_L001.sort.markdup.bam METRICS_FILE=KH15_S7_L001sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH15_S7_L002.sort.bam OUTPUT=KH15_S7_L002.sort.markdup.bam METRICS_FILE=KH15_S7_L002sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH15_S7_L003.sort.bam OUTPUT=KH15_S7_L003.sort.markdup.bam METRICS_FILE=KH15_S7_L003sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH15_S7_L004.sort.bam OUTPUT=KH15_S7_L004.sort.markdup.bam METRICS_FILE=KH15_S7_L004sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH16_S8_L001.sort.bam OUTPUT=KH16_S8_L001.sort.markdup.bam METRICS_FILE=KH16_S8_L001sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH16_S8_L002.sort.bam OUTPUT=KH16_S8_L002.sort.markdup.bam METRICS_FILE=KH16_S8_L002sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH16_S8_L003.sort.bam OUTPUT=KH16_S8_L003.sort.markdup.bam METRICS_FILE=KH16_S8_L003sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH16_S8_L004.sort.bam OUTPUT=KH16_S8_L004.sort.markdup.bam METRICS_FILE=KH16_S8_L004sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH17_S9_L001.sort.bam OUTPUT=KH17_S9_L001.sort.markdup.bam METRICS_FILE=KH17_S9_L001sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH17_S9_L002.sort.bam OUTPUT=KH17_S9_L002.sort.markdup.bam METRICS_FILE=KH17_S9_L002sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH17_S9_L003.sort.bam OUTPUT=KH17_S9_L003.sort.markdup.bam METRICS_FILE=KH17_S9_L003sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH17_S9_L004.sort.bam OUTPUT=KH17_S9_L004.sort.markdup.bam METRICS_FILE=KH17_S9_L004sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH18_S9_L001.sort.bam OUTPUT=KH18_S9_L001.sort.markdup.bam METRICS_FILE=KH18_S9_L001sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH18_S9_L002.sort.bam OUTPUT=KH18_S9_L002.sort.markdup.bam METRICS_FILE=KH18_S9_L002sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH18_S9_L003.sort.bam OUTPUT=KH18_S9_L003.sort.markdup.bam METRICS_FILE=KH18_S9_L003sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH18_S9_L004.sort.bam OUTPUT=KH18_S9_L004.sort.markdup.bam METRICS_FILE=KH18_S9_L004sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH19_S10_L001.sort.bam OUTPUT=KH19_S10_L001.sort.markdup.bam METRICS_FILE=KH19_S10_L001sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH19_S10_L002.sort.bam OUTPUT=KH19_S10_L002.sort.markdup.bam METRICS_FILE=KH19_S10_L002sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH19_S10_L003.sort.bam OUTPUT=KH19_S10_L003.sort.markdup.bam METRICS_FILE=KH19_S10_L003sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH19_S10_L004.sort.bam OUTPUT=KH19_S10_L004.sort.markdup.bam METRICS_FILE=KH19_S10_L004sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH20_S10_L001.sort.bam OUTPUT=KH20_S10_L001.sort.markdup.bam METRICS_FILE=KH20_S10_L001sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH20_S10_L002.sort.bam OUTPUT=KH20_S10_L002.sort.markdup.bam METRICS_FILE=KH20_S10_L002sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH20_S10_L003.sort.bam OUTPUT=KH20_S10_L003.sort.markdup.bam METRICS_FILE=KH20_S10_L003sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH20_S10_L004.sort.bam OUTPUT=KH20_S10_L004.sort.markdup.bam METRICS_FILE=KH20_S10_L004sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH21_S11_L001.sort.bam OUTPUT=KH21_S11_L001.sort.markdup.bam METRICS_FILE=KH21_S11_L001sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH21_S11_L002.sort.bam OUTPUT=KH21_S11_L002.sort.markdup.bam METRICS_FILE=KH21_S11_L002sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH21_S11_L003.sort.bam OUTPUT=KH21_S11_L003.sort.markdup.bam METRICS_FILE=KH21_S11_L003sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH21_S11_L004.sort.bam OUTPUT=KH21_S11_L004.sort.markdup.bam METRICS_FILE=KH21_S11_L004sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH22_S11_L001.sort.bam OUTPUT=KH22_S11_L001.sort.markdup.bam METRICS_FILE=KH22_S11_L001sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH22_S11_L002.sort.bam OUTPUT=KH22_S11_L002.sort.markdup.bam METRICS_FILE=KH22_S11_L002sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH22_S11_L003.sort.bam OUTPUT=KH22_S11_L003.sort.markdup.bam METRICS_FILE=KH22_S11_L003sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH22_S11_L004.sort.bam OUTPUT=KH22_S11_L004.sort.markdup.bam METRICS_FILE=KH22_S11_L004sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH23_S12_L001.sort.bam OUTPUT=KH23_S12_L001.sort.markdup.bam METRICS_FILE=KH23_S12_L001sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH23_S12_L002.sort.bam OUTPUT=KH23_S12_L002.sort.markdup.bam METRICS_FILE=KH23_S12_L002sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH23_S12_L003.sort.bam OUTPUT=KH23_S12_L003.sort.markdup.bam METRICS_FILE=KH23_S12_L003sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH23_S12_L004.sort.bam OUTPUT=KH23_S12_L004.sort.markdup.bam METRICS_FILE=KH23_S12_L004sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH24_S12_L001.sort.bam OUTPUT=KH24_S12_L001.sort.markdup.bam METRICS_FILE=KH24_S12_L001sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH24_S12_L002.sort.bam OUTPUT=KH24_S12_L002.sort.markdup.bam METRICS_FILE=KH24_S12_L002sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH24_S12_L003.sort.bam OUTPUT=KH24_S12_L003.sort.markdup.bam METRICS_FILE=KH24_S12_L003sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
java -Xmx8g -jar /home/kcolney/ngcchome/tools2/picard.jar MarkDuplicates INPUT=KH24_S12_L004.sort.bam OUTPUT=KH24_S12_L004.sort.markdup.bam METRICS_FILE=KH24_S12_L004sort.markdup.picardMetrics.txt REMOVE_DUPLICATES=false ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT
#--------------------------
# 4. end timer
#--------------------------
date