diff --git a/docs/api/utils.md b/docs/api/utils.md
index 886757a..551df3d 100644
--- a/docs/api/utils.md
+++ b/docs/api/utils.md
@@ -15,6 +15,7 @@ CREsted provides a few utility function to help with sequence encoding, function
     hot_encoding_to_sequence
     one_hot_encode_sequence
     fetch_sequences
+    reverse_complement
     permute_model
     setup_logging
 ```
diff --git a/src/crested/utils/__init__.py b/src/crested/utils/__init__.py
index 4440a9f..ed7a1f8 100644
--- a/src/crested/utils/__init__.py
+++ b/src/crested/utils/__init__.py
@@ -9,4 +9,5 @@
     hot_encoding_to_sequence,
     one_hot_encode_sequence,
     read_bigwig_region,
+    reverse_complement,
 )
diff --git a/src/crested/utils/_utils.py b/src/crested/utils/_utils.py
index 6a368ae..5fde197 100644
--- a/src/crested/utils/_utils.py
+++ b/src/crested/utils/_utils.py
@@ -426,3 +426,52 @@ def read_bigwig_region(
     ).squeeze()
 
     return values, positions
+
+
+def reverse_complement(sequence: str | list[str] | np.ndarray) -> str | np.ndarray:
+    """
+    Perform reverse complement on either a one-hot encoded array or a (list of) DNA sequence string(s).
+
+    Parameters
+    ----------
+    sequence
+        The DNA sequence string(s) or one-hot encoded array to reverse complement.
+
+    Returns
+    -------
+    The reverse complemented DNA sequence string or one-hot encoded array.
+    """
+
+    def complement_str(seq: str) -> str:
+        complement = str.maketrans("ACGTacgt", "TGCAtgca")
+        return seq.translate(complement)[::-1]
+
+    if isinstance(sequence, str):
+        return complement_str(sequence)
+    elif isinstance(sequence, list):
+        return [complement_str(seq) for seq in sequence]
+    elif isinstance(sequence, np.ndarray):
+        if sequence.ndim == 2:
+            if sequence.shape[1] == 4:
+                return sequence[::-1, ::-1]
+            elif sequence.shape[0] == 4:
+                return sequence[:, ::-1][:, ::-1]
+            else:
+                raise ValueError(
+                    "One-hot encoded array must have shape (W, 4) or (4, W)"
+                )
+        elif sequence.ndim == 3:
+            if sequence.shape[1] == 4:
+                return sequence[:, ::-1, ::-1]
+            elif sequence.shape[2] == 4:
+                return sequence[:, ::-1, ::-1]
+            else:
+                raise ValueError(
+                    "One-hot encoded array must have shape (B, 4, W) or (B, W, 4)"
+                )
+        else:
+            raise ValueError("One-hot encoded array must have 2 or 3 dimensions")
+    else:
+        raise TypeError(
+            "Input must be either a DNA sequence string or a one-hot encoded array"
+        )
diff --git a/tests/test_utils.py b/tests/test_utils.py
new file mode 100644
index 0000000..675befa
--- /dev/null
+++ b/tests/test_utils.py
@@ -0,0 +1,35 @@
+import numpy as np
+
+from crested.utils import (
+    hot_encoding_to_sequence,
+    one_hot_encode_sequence,
+    reverse_complement,
+)
+
+
+def test_reverse_complement_string():
+    assert reverse_complement("ACGT") == "ACGT"
+    assert reverse_complement("TGCA") == "TGCA"
+    assert reverse_complement("AAGGTTCC") == "GGAACCTT"
+
+
+def test_reverse_complement_list_of_strings():
+    assert reverse_complement(["ACGT", "TGCA"]) == ["ACGT", "TGCA"]
+    assert reverse_complement(["AAGGTTCC", "CCGGAATT"]) == ["GGAACCTT", "AATTCCGG"]
+
+
+def test_reverse_complement_one_hot_encoded_array():
+    seq_1 = "ACGTA"
+    seq_1_one_hot = one_hot_encode_sequence(seq_1)
+    seq_1_rev = reverse_complement(seq_1)
+    seq_1_rev_one_hot = one_hot_encode_sequence(seq_1_rev)
+
+    np.testing.assert_array_equal(reverse_complement(seq_1_one_hot), seq_1_rev_one_hot)
+
+    seq_2 = "AAGGTTCC"
+    seq_2_rev = "GGAACCTT"
+    seq_2_one_hot = one_hot_encode_sequence(seq_2, expand_dim=False)
+    seq_2_rev_one_hot = reverse_complement(seq_2_one_hot)
+    seq_2_seq_reversed = hot_encoding_to_sequence(seq_2_rev_one_hot)
+
+    assert seq_2_seq_reversed == seq_2_rev