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Bad input data sent to tcoffee #51

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aflaus opened this issue Apr 13, 2020 · 2 comments
Open

Bad input data sent to tcoffee #51

aflaus opened this issue Apr 13, 2020 · 2 comments
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@aflaus
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aflaus commented Apr 13, 2020

  1. Download appeared to complete successfully (see attached log)
  2. Cannot build catalogue or manuscript due to problem with sequences being sent to tcoffee.
  3. Can build 'data' (see attached output).

scons: Building targets ...
$ perl '-Ilib-perl5' '-Iscripts' '-MHistoneSequencesDB' '-e' 'HistoneSequencesDB->new("results/sequences")->write_db("results/histones_db.store")'
$ perl '-Ilib-perl5' '-Iscripts' 'scripts/align_proteins.pl' 'results/histones_db.store' 'H2A' 'results/aligned_H2A_proteins.fasta'

--------------------- WARNING ---------------------
MSG: passed an array ref which did not contain objects to _setinput

------------- EXCEPTION: Bio::Root::Exception -------------
MSG: Bad input data or less than 2 sequences in ARRAY(0x561294f4c138) !
STACK: Error::throw
STACK: Bio::Root::Root::throw /usr/share/perl5/Bio/Root/Root.pm:449
STACK: Bio::Tools::Run::Alignment::TCoffee::align /usr/share/perl5/Bio/Tools/Run/Alignment/TCoffee.pm:161
STACK: scripts/align_proteins.pl:87

scons: *** [results/aligned_H2A_proteins.fasta] Error 25
scons: building terminated because of errors.

extractor.log
output-of-data.txt

@haleymac
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I also experienced this error when attempting to run in virtual box

@aflaus
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aflaus commented Oct 24, 2023

Apologies but irrespective of the likely broken dependency, this set of scripts also relies on an outdated histone gene nomenclature. I hope to revisit this in the future but it will require a major rewrite sorry,

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