diff --git a/src/controller/python/chip/ChipStack.py b/src/controller/python/chip/ChipStack.py
index 06afff3ef3c380..b47c4639825da8 100644
--- a/src/controller/python/chip/ChipStack.py
+++ b/src/controller/python/chip/ChipStack.py
@@ -144,8 +144,6 @@ class ChipStack(object):
     def __init__(self, persistentStoragePath: str, enableServerInteractions=True):
         builtins.enableDebugMode = False
 
-        # TODO: Probably no longer necessary, see https://github.com/project-chip/connectedhomeip/issues/33321.
-        self.networkLock = Lock()
         self.completeEvent = Event()
         self.commissioningCompleteEvent = Event()
         self._ChipStackLib = None
@@ -212,7 +210,6 @@ def Shutdown(self):
         # #20437 tracks consolidating these.
         #
         self._ChipStackLib.pychip_CommonStackShutdown()
-        self.networkLock = None
         self.completeEvent = None
         self._ChipStackLib = None
         self._chipDLLPath = None
@@ -226,10 +223,7 @@ def Call(self, callFunct, timeoutMs: int = None):
         This function is a wrapper of PostTaskOnChipThread, which includes some handling of application specific logics.
         Calling this function on CHIP on CHIP mainloop thread will cause deadlock.
         '''
-        # TODO: Lock probably no longer necessary, see https://github.com/project-chip/connectedhomeip/issues/33321.
-        with self.networkLock:
-            res = self.PostTaskOnChipThread(callFunct).Wait(timeoutMs)
-        return res
+        return self.PostTaskOnChipThread(callFunct).Wait(timeoutMs)
 
     async def CallAsync(self, callFunct, timeoutMs: int = None):
         '''Run a Python function on CHIP stack, and wait for the response.
@@ -256,9 +250,7 @@ def CallAsyncWithCompleteCallback(self, callFunct):
         # throw error if op in progress
         self.callbackRes = None
         self.completeEvent.clear()
-        # TODO: Lock probably no longer necessary, see https://github.com/project-chip/connectedhomeip/issues/33321.
-        with self.networkLock:
-            res = self.PostTaskOnChipThread(callFunct).Wait()
+        res = self.PostTaskOnChipThread(callFunct).Wait()
 
         if not res.is_success:
             self.completeEvent.set()