diff --git a/studio/app/optinist/wrappers/optinist/visualize_utils/params/roi_fluo_from_hdf5.yaml b/studio/app/optinist/wrappers/optinist/visualize_utils/params/roi_fluo_from_hdf5.yaml
index e16c76dff..e498b8467 100644
--- a/studio/app/optinist/wrappers/optinist/visualize_utils/params/roi_fluo_from_hdf5.yaml
+++ b/studio/app/optinist/wrappers/optinist/visualize_utils/params/roi_fluo_from_hdf5.yaml
@@ -1 +1,4 @@
-""
+# This is a temporary measure. Generally, Transpose should be set to True.
+# However, when Transpose is set to True, the data coordinates may sometimes be reversed.
+# Therefore, it may be switched to False on the screen.
+transpose: True
diff --git a/studio/app/optinist/wrappers/optinist/visualize_utils/roi_fluo_from_hdf5.py b/studio/app/optinist/wrappers/optinist/visualize_utils/roi_fluo_from_hdf5.py
index 3d9de2291..036df2fa6 100644
--- a/studio/app/optinist/wrappers/optinist/visualize_utils/roi_fluo_from_hdf5.py
+++ b/studio/app/optinist/wrappers/optinist/visualize_utils/roi_fluo_from_hdf5.py
@@ -36,7 +36,11 @@ def roi_fluo_from_hdf5(
     all_roi = RoiData(
         np.nanmax(cell_img.data, axis=0), output_dir=output_dir, file_name="all_roi"
     )
-    fluorescence = FluoData(np.transpose(fluo.data), file_name="fluorescence")
+
+    if params["transpose"]:
+        fluorescence = FluoData(np.transpose(fluo.data), file_name="fluorescence")
+    else:
+        fluorescence = FluoData(fluo.data, file_name="fluorescence")
 
     if iscell is None:
         return {"all_roi": all_roi, "fluorescence": fluorescence}