diff --git a/data/outputs/assay_protocols.yaml b/data/outputs/assay_protocols.yaml index c198496b..38f0327e 100644 --- a/data/outputs/assay_protocols.yaml +++ b/data/outputs/assay_protocols.yaml @@ -2,8 +2,8 @@ permalink: http://asapdiscovery.org/outputs/assay-protocols/#MERS-CoV_Mpro_fluorescence-assay name: MERS-CoV main protease (Mpro) activity fluorescence dose response biochemical assay description: | - This is a functional, biochemical assay used to identify treatments for viral infectious disease in MERS 3C-like main viral protease. - Utilizing a direct enzyme activity measurement method, the experiment was performed in a 384-well plate reading the fluorescence intensity. This assay tested the mode of action of inhibition. + This biochemical assay measures the inhibition of protease activity of the MERS-CoV main viral protease (Mpro), also known as the 3C-like protease (3CLpro). + It is suitable for high-throughput dose-response measurements in a 384-well plates for supporting drug discovery efforts against this target. links: - name: assay protocol url: https://www.protocols.io/view/mers-main-protease-mpro-fluorescence-dose-response-eq2ly7r1rlx9 @@ -17,14 +17,16 @@ permalink: http://asapdiscovery.org/outputs/assay-protocols/#SARS-CoV-2_Mpro_fluorescence-assay name: SARS-CoV-2 main protease (Mpro) activity fluorescence dose response biochemical assay description: | - This is a functional, biochemical assay used to identify treatments for viral infectious disease that target SARS-CoV-2 3C-like main viral protease (MPro). - Utilizing a direct enzyme activity measurement method, the experiment was performed in a 384-well plate reading the fluorescence intensity. This assay tested the mode of action of inhibition. + This biochemical assay measures the inhibition of protease activity of the SARS-CoV-2 main viral protease (Mpro), also known as the 3C-like protease (3CLpro). + It is suitable for high-throughput dose-response measurements in a 384-well plates for supporting drug discovery efforts against this target. links: - name: assay protocol - url: https://www.protocols.io/view/sars-cov-2-main-protease-mpro-fluorescence-dose-re-81wgbye9nvpk + url: https://www.protocols.io/view/sars-cov-2-mpro-fluorescence-dose-response-81wgbye9nvpk type: biochemical-assay-protocol cores: [Biochemical Assay Core] events: + - date: 2024-04-28 + description: draft v4 - date: 2023-04-28 description: draft v3