Problem in Bin_refinement module_IOError: [Errno2] No such file or directory: binsA.checkm/storage/tree/concatenated.tre

[mamunimsfcu16]

Dear metawrap Experts,
Good day!
I have encountered this issue using my personal computer (24GB RAM). I'm new user and trying to learn from tutorial data (3 samples in the metaWRAP tutorial). I hope, I can get some help to fix this issue.
Thanks.

Best regards
Mamun

[Zk-linux]

Hi~ I think the problem is that the pplacer implemented in the checkm needs a large amount of memory (40G at least), and you can use the "--reduced_tree" to reduce the RAM requirement.
https://github.com/Ecogenomics/CheckM/wiki/Installation#system-requirements

[mamunimsfcu16]

Thank you very much for your email. I will try it again. Best regards Mamun

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On Mon, Jul 15, 2024 at 12:59 AM Zk-linux ***@***.***> wrote: Hi~ I think the problem is that the pplacer implemented in the checkm needs a large amount of memory (40G at least), and you can use the "--reduced_tree" to reduce the RAM requirement. — Reply to this email directly, view it on GitHub <#545 (comment)>, or unsubscribe <https://github.com/notifications/unsubscribe-auth/AIYJZTBXII3E4SJJX5U4FY3ZMKU5RAVCNFSM6AAAAABI35IY6CVHI2DSMVQWIX3LMV43OSLTON2WKQ3PNVWWK3TUHMZDEMRXGQYTGNRQHE> . You are receiving this because you authored the thread.Message ID: ***@***.***>