diff --git a/scpipelines/seurat/pipeline_qc-1/Rmarkdown/QC.Rmd b/scpipelines/seurat/pipeline_qc-1/Rmarkdown/QC.Rmd
index ee8c03b..f816d6b 100755
--- a/scpipelines/seurat/pipeline_qc-1/Rmarkdown/QC.Rmd
+++ b/scpipelines/seurat/pipeline_qc-1/Rmarkdown/QC.Rmd
@@ -191,7 +191,7 @@ for(i in 1:length(so)){
     
     # run emptydrops
     set.seed(1)
-    out <- DropletUtils::emptyDrops(assay(sce[[i]], "counts"), lower = 100)
+    out <- DropletUtils::emptyDrops(assay(sce[[i]], "counts"), lower = ini$UMI_filter)
     
     # add emptydrops info to colData
     sce[[i]]@colData$empty_drops_FDR <- out$FDR
diff --git a/scpipelines/seurat/pipeline_qc-1/pipeline.yml b/scpipelines/seurat/pipeline_qc-1/pipeline.yml
index 7946012..ae16a61 100644
--- a/scpipelines/seurat/pipeline_qc-1/pipeline.yml
+++ b/scpipelines/seurat/pipeline_qc-1/pipeline.yml
@@ -17,7 +17,7 @@ mirror: useast
 so_filter: 100
 
 #Specificy the number of counts if more stringent filter is required
-UMI_filter: 100
+UMI_filter: 300
 
 #Specify the format of output files
 output: seurat_object