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For future analysis of mantis datasets, the following order of steps will facilitate the processing:
Conversion to Zarr (light-sheet and label-free)
Label-free reconstruction
Deskew of fluorescence channels
Z and XY drift stabilization - register timepoints (typically in LF arm) to t=0
Registration - register moving dataset to t=0 of fixed dataset
-- We need to VS one FOV for registration
-- the registered datasets will have the voxel size of the fixed dataset
-- fixed channel should be the one with lower spacial resolution such that the moving channel is resamples in that space
-- if fixed and moving datasets have comparable resolution, the moving dataset should be the one with fewer channels such that less data is interpolated
** Where should we be merging the datasets and if that is necessary at all (i.e step 5)?
The folder structure should follow something like below. Additionally, the VS steps always output the scale metadata as (1,1,1,1,1), which by default should be the input dataset's scale (here)
The text was updated successfully, but these errors were encountered:
For future analysis of mantis datasets, the following order of steps will facilitate the processing:
-- We need to VS one FOV for registration
-- the registered datasets will have the voxel size of the fixed dataset
-- fixed channel should be the one with lower spacial resolution such that the moving channel is resamples in that space
-- if fixed and moving datasets have comparable resolution, the moving dataset should be the one with fewer channels such that less data is interpolated
Merging phase and fluorescence **** Where should we be merging the datasets and if that is necessary at all (i.e step 5)?
The folder structure should follow something like below. Additionally, the VS steps always output the scale metadata as (1,1,1,1,1), which by default should be the input dataset's scale (here)
The text was updated successfully, but these errors were encountered: