TWZLR_primers.json

{
     "Layer 1": {
          "Homology left-ampR-gfp": [
               "CAGGCCTGCCAGGCAA",
               "AGGCGCGGGGGAGATAAGAGTGCCGGATCTTTTTCCTGA",
               "GCACTCTTATCTCCCCCGCGCC",
               "CAGTGAAAAGTTCTTCTCCTTTACTCATTTGGTTCGACCCCATTTGCC",
               "GGCAAATGGGGTCGAACCAAATGAGTAAAGGAGAAGAACTTTTCACTG",
               "TCAACAAGAATTGGGACAACTCC"
          ],
          "Homology left-PA0056-gfp": [
               "CAGGCCTGCCAGGCAA",
               "CCTGGCGCTGGCGTGAGAGTGCCGGATCTTTTTCCTGA",
               "CACTCTCACGCCAGCGCCAG",
               "CAGTGAAAAGTTCTTCTCCTTTACTCATATGGACCGCCTGACCGC",
               "GCGGTCAGGCGGTCCATATGAGTAAAGGAGAAGAACTTTTCACTG",
               "TCAACAAGAATTGGGACAACTCC"
          ]
     },
     "Layer 2": {
          "ampR-gfp-Homology Right": [
               "GAAGCTTCGAAGGCGCG",
               "CAGTGAAAAGTTCTTCTCCTTTACTCATTTGGTTCGACCCCATTTGCC",
               "GGGTCGAACCAAATGAGTAAAGGAGAAGAACTTTTCACT",
               "CACCCTCCACAATGTATAGTTCAGATTATTTGTATAGTTCATCCATGCCATGT",
               "ACATGGCATGGATGAACTATACAAATAATCTGAACTATACATTGTGGAGGGTG",
               "TGCTTAGCACTACCGCCC"
          ],
          "PA0056-gfp-Homology Right": [
               "CCGCTGTCGATGACTTCGA",
               "CAGTGAAAAGTTCTTCTCCTTTACTCATATGGACCGCCTGACCGC",
               "CAGGCGGTCCATATGAGTAAAGGAGAAGAACTTTTCACT",
               "CACCCTCCACAATGTATAGTTCAGATTATTTGTATAGTTCATCCATGCCATGT",
               "ACATGGCATGGATGAACTATACAAATAATCTGAACTATACATTGTGGAGGGTG",
               "TGCTTAGCACTACCGCCC"
          ]
     }
}