diff --git a/src/System.Windows.Forms/System/Windows/Forms/Controls/ListView/ListView.cs b/src/System.Windows.Forms/System/Windows/Forms/Controls/ListView/ListView.cs
index f290e8ce8eb..c1d2a1f03b9 100644
--- a/src/System.Windows.Forms/System/Windows/Forms/Controls/ListView/ListView.cs
+++ b/src/System.Windows.Forms/System/Windows/Forms/Controls/ListView/ListView.cs
@@ -208,6 +208,9 @@ public partial class ListView : Control
     // We cache the NewWidth supplied by the user and use it on HDN_ENDTRACK to set the final column width.
     private int _newWidthForColumnWidthChangingCancelled = -1;
 
+    // Tracks indices of ListViewItems that have been accessed by UIA in virtual mode,
+    // so their UIA providers can be properly released later without triggering item retrieval.
+    private readonly HashSet<int> _uiaAccessedIndices = [];
     /// <summary>
     ///  Creates an empty ListView with default styles.
     /// </summary>
@@ -5084,6 +5087,14 @@ public void RedrawItems(int startIndex, int endIndex, bool invalidateOnly)
         }
     }
 
+    internal void NotifyUiaCreated(int index)
+    {
+        if (VirtualMode)
+        {
+            _uiaAccessedIndices.Add(index);
+        }
+    }
+
     internal override void ReleaseUiaProvider(HWND handle)
     {
         if (!OsVersion.IsWindows8OrGreater())
@@ -5091,9 +5102,21 @@ internal override void ReleaseUiaProvider(HWND handle)
             return;
         }
 
-        for (int i = 0; i < Items.Count; i++)
+        if (VirtualMode)
+        {
+            foreach (int index in _uiaAccessedIndices)
+            {
+                Items.GetItemByIndex(index)?.ReleaseUiaProvider();
+            }
+
+            _uiaAccessedIndices.Clear();
+        }
+        else
         {
-            Items.GetItemByIndex(i)?.ReleaseUiaProvider();
+            for (int i = 0; i < Items.Count; i++)
+            {
+                Items.GetItemByIndex(i)?.ReleaseUiaProvider();
+            }
         }
 
         if (_defaultGroup is not null)
diff --git a/src/System.Windows.Forms/System/Windows/Forms/Controls/ListView/ListViewItem.cs b/src/System.Windows.Forms/System/Windows/Forms/Controls/ListView/ListViewItem.cs
index 3c8bf59e6ee..a6e44dd7233 100644
--- a/src/System.Windows.Forms/System/Windows/Forms/Controls/ListView/ListViewItem.cs
+++ b/src/System.Windows.Forms/System/Windows/Forms/Controls/ListView/ListViewItem.cs
@@ -265,6 +265,8 @@ internal virtual AccessibleObject AccessibilityObject
                 };
             }
 
+            owningListView.NotifyUiaCreated(Index);
+
             return _accessibilityObject;
         }
     }