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Hi,
I tried to use MethylDackel extract on a dataset aligned with bwameth, and I used -l to provide a bed file.
The command I used is : MethylDackel extract --cytosine_report --CHG --CHH -l R012V1_Probe.bed -o Nova683-RD-R012V1-200827-LCM-Index100-cap08 hg19.fa Nova683-RD-R012V1-200827-LCM-Index100-cap08.sorted.bam
But it seems the positions in Nova683-RD-R012V1-200827-LCM-Index100-cap08.cytosine_report.txt did not match the regions in providing bed file.
While Bedfile regions be like this:
Hi,
I tried to use MethylDackel extract on a dataset aligned with bwameth, and I used -l to provide a bed file.
The command I used is :
MethylDackel extract --cytosine_report --CHG --CHH -l R012V1_Probe.bed -o Nova683-RD-R012V1-200827-LCM-Index100-cap08 hg19.fa Nova683-RD-R012V1-200827-LCM-Index100-cap08.sorted.bam
But it seems the positions in Nova683-RD-R012V1-200827-LCM-Index100-cap08.cytosine_report.txt did not match the regions in providing bed file.
While Bedfile regions be like this:
The Nova683-RD-R012V1-200827-LCM-Index100-cap08.cytosine_report.txt is:
There are lots of positions beyond my giving regions.
Is that any problem with my command?
Thank you so much! :)
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