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fasta2DB.c
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fasta2DB.c
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/*******************************************************************************************
*
* Add .fasta files to a DB:
* Adds the given fasta files in the given order to <path>.db. If the db does not exist
* then it is created. All .fasta files added to a given data base must have the same
* header format and follow Pacbio's convention. A file cannot be added twice and this
* is enforced. The command either builds or appends to the .<path>.idx and .<path>.bps
* files, where the index file (.idx) contains information about each read and their offsets
* in the base-pair file (.bps) that holds the sequences where each base is compessed
* into 2-bits. The two files are hidden by virtue of their names beginning with a '.'.
* <path>.db is effectively a stub file with given name that contains an ASCII listing
* of the files added to the DB and possibly the block partitioning for the DB if DBsplit
* has been called upon it.
*
* Author: Gene Myers
* Date : May 2013
* Modify: DB upgrade: now *add to* or create a DB depending on whether it exists, read
* multiple .fasta files (no longer a stdin pipe).
* Date : April 2014
*
********************************************************************************************/
#include <stdlib.h>
#include <stdio.h>
#include <string.h>
#include <strings.h>
#include <sys/stat.h>
#include <unistd.h>
#include "DB.h"
#ifdef HIDE_FILES
#define PATHSEP "/."
#else
#define PATHSEP "/"
#endif
static char *Usage = "[-v] <path:string> <input:fasta> ...";
static char number[128] =
{ 0, 0, 0, 0, 0, 0, 0, 0,
0, 0, 0, 0, 0, 0, 0, 0,
0, 0, 0, 0, 0, 0, 0, 0,
0, 0, 0, 0, 0, 0, 0, 0,
0, 0, 0, 0, 0, 0, 0, 0,
0, 0, 0, 0, 0, 0, 0, 0,
0, 0, 0, 0, 0, 0, 0, 0,
0, 0, 0, 0, 0, 0, 0, 0,
0, 0, 0, 1, 0, 0, 0, 2,
0, 0, 0, 0, 0, 0, 0, 0,
0, 0, 0, 0, 3, 0, 0, 0,
0, 0, 0, 0, 0, 0, 0, 0,
0, 0, 0, 1, 0, 0, 0, 2,
0, 0, 0, 0, 0, 0, 0, 0,
0, 0, 0, 0, 3, 0, 0, 0,
0, 0, 0, 0, 0, 0, 0, 0,
};
int main(int argc, char *argv[])
{ FILE *istub, *ostub;
char *dbname;
char *root, *pwd;
FILE *bases, *indx;
int64 boff, ioff;
int ifiles, ofiles;
char **flist;
HITS_DB db;
int oreads;
int64 offset;
int VERBOSE;
// Usage: <path:string> <input:fasta> ...
{ int i, j, k;
int flags[128];
ARG_INIT("fasta2DB")
j = 1;
for (i = 1; i < argc; i++)
if (argv[i][0] == '-')
{ ARG_FLAGS("v") }
else
argv[j++] = argv[i];
argc = j;
VERBOSE = flags['v'];
if (argc <= 2)
{ fprintf(stderr,"Usage: %s %s\n",Prog_Name,Usage);
exit (1);
}
}
// Try to open DB file, if present then adding to DB, otherwise creating new DB. Set up
// variables as follows:
// dbname = full name of db = <pwd>/<root>.db
// istub = open db file (if adding) or NULL (if creating)
// ostub = new image of db file (will overwrite old image at end)
// bases = .bps file positioned for appending
// indx = .idx file positioned for appending
// oreads = # of reads currently in db
// offset = offset in .bps at which to place next sequence
// ioff = offset in .idx file to truncate to if command fails
// boff = offset in .bps file to truncate to if command fails
// ifiles = # of .fasta files to add
// ofiles = # of .fasta files already in db
// flist = [0..ifiles+ofiles] list of file names (root only) added to db so far
{ int i;
root = Root(argv[1],".db");
pwd = PathTo(argv[1]);
dbname = Strdup(Catenate(pwd,"/",root,".db"),"Allocating db name");
if (dbname == NULL)
exit (1);
istub = fopen(dbname,"r");
ifiles = argc-2;
if (istub == NULL)
{ ofiles = 0;
bases = Fopen(Catenate(pwd,PATHSEP,root,".bps"),"w+");
indx = Fopen(Catenate(pwd,PATHSEP,root,".idx"),"w+");
if (bases == NULL || indx == NULL)
exit (1);
fwrite(&db,sizeof(HITS_DB),1,indx);
oreads = 0;
offset = 0;
boff = 0;
ioff = 0;
}
else
{ fscanf(istub,DB_NFILE,&ofiles);
bases = Fopen(Catenate(pwd,PATHSEP,root,".bps"),"r+");
indx = Fopen(Catenate(pwd,PATHSEP,root,".idx"),"r+");
if (bases == NULL || indx == NULL)
exit (1);
fread(&db,sizeof(HITS_DB),1,indx);
fseeko(bases,0,SEEK_END);
fseeko(indx, 0,SEEK_END);
oreads = db.oreads;
offset = ftello(bases);
boff = offset;
ioff = ftello(indx);
}
flist = (char **) Malloc(sizeof(char *)*(ofiles+ifiles),"Allocating file list");
ostub = Fopen(Catenate(pwd,"/",root,".dbx"),"w");
if (ostub == NULL || flist == NULL)
exit (1);
fprintf(ostub,DB_NFILE,ofiles+ifiles);
for (i = 0; i < ofiles; i++)
{ int last;
char prolog[MAX_NAME], fname[MAX_NAME];
fscanf(istub,DB_FDATA,&last,fname,prolog);
if ((flist[i] = Strdup(fname,"Adding to file list")) == NULL)
goto error;
fprintf(ostub,DB_FDATA,last,fname,prolog);
}
}
{ int maxlen;
int64 totlen, count[4];
int pmax, rmax;
HITS_READ *prec;
char *read;
int c;
// Buffer for reads all in the same well
pmax = 100;
prec = (HITS_READ *) Malloc(sizeof(HITS_READ)*pmax,"Allocating record buffer");
if (prec == NULL)
goto error;
// Buffer for accumulating .fasta sequence over multiple lines
rmax = MAX_NAME + 60000;
read = (char *) Malloc(rmax+1,"Allocating line buffer");
if (read == NULL)
goto error;
totlen = 0; // total # of bases in new .fasta files
maxlen = 0; // longest read in new .fasta files
for (c = 0; c < 4; c++) // count of acgt in new .fasta files
count[c] = 0;
// For each new .fasta file do:
for (c = 2; c < argc; c++)
{ FILE *input;
char *path, *core, *prolog;
int nline, eof, rlen, pcnt;
int pwell;
// Open it: <path>/<core>.fasta, check that core is not too long, and add it to
// list of added files, flist[0..ofiles), after checking that it is not
// already in the list.
path = PathTo(argv[c]);
core = Root(argv[c],".fasta");
if ((input = Fopen(Catenate(path,"/",core,".fasta"),"r")) == NULL)
goto error;
if (strlen(core) >= MAX_NAME)
{ fprintf(stderr,"%s: File name over %d chars: '%.200s'\n",
Prog_Name,MAX_NAME,core);
goto error;
}
{ int j;
for (j = 0; j < ofiles; j++)
if (strcmp(core,flist[j]) == 0)
{ fprintf(stderr,"%s: File %s.fasta is already in database %s.db\n",
Prog_Name,core,Root(argv[1],".db"));
goto error;
}
flist[ofiles++] = core;
free(path);
}
if (VERBOSE)
{ fprintf(stderr,"Adding '%s' ...\n",core);
fflush(stderr);
}
// Get the header of the first line, check that it has PACBIO format, and record
// prolog in 'prolog'.
pcnt = 0;
rlen = 0;
nline = 1;
eof = (fgets(read,MAX_NAME,input) == NULL);
if (read[strlen(read)-1] != '\n')
{ fprintf(stderr,"File %s.fasta, Line 1: Fasta line is too long (> %d chars)\n",
core,MAX_NAME-2);
goto error;
}
if (!eof && read[0] != '>')
{ fprintf(stderr,"File %s.fasta, Line 1: First header in fasta file is missing\n",core);
goto error;
}
{ char *find;
int well, beg, end, qv;
find = index(read+1,'/');
if (find != NULL && sscanf(find+1,"%d/%d_%d RQ=0.%d\n",&well,&beg,&end,&qv) >= 3)
{ *find = '\0';
prolog = Strdup(read+1,"Extracting prolog");
*find = '/';
if (prolog == NULL) goto error;
}
else
{ fprintf(stderr,"File %s.fasta, Line %d: Pacbio header line format error\n",
core,nline);
goto error;
}
}
// Read in all the sequences until end-of-file
{ int i, x;
pwell = -1;
while (!eof)
{ int beg, end, clen;
int well, qv;
char *find;
find = index(read+(rlen+1),'/');
if (find == NULL)
{ fprintf(stderr,"File %s.fasta, Line %d: Pacbio header line format error\n",
core,nline);
goto error;
}
*find = '\0';
if (strcmp(read+(rlen+1),prolog) != 0)
{ fprintf(stderr,"File %s.fasta, Line %d: Pacbio header line name inconsisten\n",
core,nline);
goto error;
}
*find = '/';
x = sscanf(find+1,"%d/%d_%d RQ=0.%d\n",&well,&beg,&end,&qv);
if (x < 3)
{ fprintf(stderr,"File %s.fasta, Line %d: Pacbio header line format error\n",
core,nline);
goto error;
}
else if (x == 3)
qv = 0;
rlen = 0;
while (1)
{ eof = (fgets(read+rlen,MAX_NAME,input) == NULL);
nline += 1;
x = strlen(read+rlen)-1;
if (read[rlen+x] != '\n')
{ fprintf(stderr,"File %s.fasta, Line %d:",core,nline);
fprintf(stderr," Fasta line is too long (> %d chars)\n",MAX_NAME-2);
goto error;
}
if (eof || read[rlen] == '>')
break;
rlen += x;
if (rlen + MAX_NAME > rmax)
{ rmax = 1.2 * rmax + 1000 + MAX_NAME;
read = (char *) realloc(read,rmax+1);
if (read == NULL)
{ fprintf(stderr,"File %s.fasta, Line %d:",core,nline);
fprintf(stderr," Out of memory (Allocating line buffer)\n");
goto error;
}
}
}
read[rlen] = '\0';
for (i = 0; i < rlen; i++)
{ x = number[(int) read[i]];
count[x] += 1;
read[i] = x;
}
oreads += 1;
totlen += rlen;
if (rlen > maxlen)
maxlen = rlen;
prec[pcnt].origin = well;
prec[pcnt].beg = beg;
prec[pcnt].end = end;
prec[pcnt].boff = offset;
prec[pcnt].coff = 0;
prec[pcnt].flags = qv;
Compress_Read(rlen,read);
clen = COMPRESSED_LEN(rlen);
fwrite(read,1,clen,bases);
offset += clen;
if (pwell == well)
{ prec[pcnt].flags |= DB_CSS;
pcnt += 1;
if (pcnt >= pmax)
{ pmax = pcnt*1.2 + 100;
prec = (HITS_READ *) realloc(prec,sizeof(HITS_READ)*pmax);
if (prec == NULL)
{ fprintf(stderr,"File %s.fasta, Line %d: Out of memory",core,nline);
fprintf(stderr," (Allocating read records)\n");
goto error;
}
}
}
else if (pcnt == 0)
pcnt += 1;
else
{ x = 0;
for (i = 1; i < pcnt; i++)
if (prec[i].end - prec[i].beg > prec[x].end - prec[x].beg)
x = i;
prec[x].flags |= DB_BEST;
fwrite(prec,sizeof(HITS_READ),pcnt,indx);
prec[0] = prec[pcnt];
pcnt = 1;
}
pwell = well;
}
// Complete processing of .fasta file: flush last well group, write file line
// in db image, free prolog, and close file
x = 0;
for (i = 1; i < pcnt; i++)
if (prec[i].end - prec[i].beg > prec[x].end - prec[i].beg)
x = i;
prec[x].flags |= DB_BEST;
fwrite(prec,sizeof(HITS_READ),pcnt,indx);
fprintf(ostub,DB_FDATA,oreads,core,prolog);
free(prolog);
fclose(input);
}
}
// Finished loading all sequences: update relevant fields in db record
db.oreads = oreads;
if (istub == NULL)
{ for (c = 0; c < 4; c++)
db.freq[c] = (1.*count[c])/totlen;
db.totlen = totlen;
db.maxlen = maxlen;
db.cutoff = -1;
}
else
{ for (c = 0; c < 4; c++)
db.freq[c] = (db.freq[c]*db.totlen + (1.*count[c]))/(db.totlen + totlen);
db.totlen += totlen;
if (maxlen > db.maxlen)
db.maxlen = maxlen;
}
}
// If db has been previously partitioned then calculate additional partition points and
// write to new db file image
if (db.cutoff >= 0)
{ int64 totlen, dbpos, size;
int nblock, ireads, bfirst, rlen;
int ofirst, cutoff, allflag;
HITS_READ record;
int i;
if (VERBOSE)
{ fprintf(stderr,"Updating block partition ...\n");
fflush(stderr);
}
// Read the block portion of the existing db image getting the indices of the first
// read in the last block of the exisiting db as well as the partition parameters.
// Copy the old image block information to the new block information (except for
// the indices of the last partial block)
fscanf(istub,DB_NBLOCK,&nblock);
dbpos = ftello(ostub);
fprintf(ostub,DB_NBLOCK,0);
fscanf(istub,DB_PARAMS,&size,&cutoff,&allflag);
fprintf(ostub,DB_PARAMS,size,cutoff,allflag);
if (allflag)
allflag = 0;
else
allflag = DB_BEST;
size *= 1000000ll;
nblock -= 1;
for (i = 0; i <= nblock; i++)
{ fscanf(istub,DB_BDATA,&ofirst,&bfirst);
fprintf(ostub,DB_BDATA,ofirst,bfirst);
}
// Seek the first record of the last block of the existing db in .idx, and then
// compute and record partition indices for the rest of the db from this point
// forward.
fseeko(indx,sizeof(HITS_DB)+sizeof(HITS_READ)*ofirst,SEEK_SET);
totlen = 0;
ireads = 0;
for (i = ofirst; i < oreads; i++)
{ fread(&record,sizeof(HITS_READ),1,indx);
rlen = record.end - record.beg;
if (rlen >= cutoff && (record.flags & DB_BEST) >= allflag)
{ ireads += 1;
bfirst += 1;
totlen += rlen;
if (totlen >= size || ireads >= READMAX)
{ fprintf(ostub," %9d %9d\n",i+1,bfirst);
totlen = 0;
ireads = 0;
nblock += 1;
}
}
}
if (ireads > 0)
{ fprintf(ostub,DB_BDATA,oreads,bfirst);
nblock += 1;
}
db.breads = bfirst;
fseeko(ostub,dbpos,SEEK_SET);
fprintf(ostub,DB_NBLOCK,nblock); // Rewind and record the new number of blocks
}
else
db.breads = oreads;
rewind(indx);
fwrite(&db,sizeof(HITS_DB),1,indx); // Write the finalized db record into .idx
if (istub != NULL)
fclose(istub);
fclose(ostub);
fclose(indx);
fclose(bases);
rename(Catenate(pwd,"/",root,".dbx"),dbname); // New image replaces old image
exit (0);
// Error exit: Either truncate or remove the .idx and .bps files as appropriate.
// Remove the new image file <pwd>/<root>.dbx
error:
if (ioff != 0)
{ fseeko(indx,0,SEEK_SET);
ftruncate(fileno(indx),ioff);
}
if (boff != 0)
{ fseeko(bases,0,SEEK_SET);
ftruncate(fileno(bases),boff);
}
fclose(indx);
fclose(bases);
if (ioff == 0)
unlink(Catenate(pwd,PATHSEP,root,".idx"));
if (boff == 0)
unlink(Catenate(pwd,PATHSEP,root,".bps"));
if (istub != NULL)
fclose(istub);
fclose(ostub);
unlink(Catenate(pwd,"/",root,".dbx"));
exit (1);
}