No issue. Curious about applying HiSig to my own project.

[ttessie2]

Hi Fan,
I'll preface this by saying I am not a bioinformatician. I have a question regarding applying HiSig/CliXO to my own research. I have proteomics data that I've acquired during the course of viral replication. I'm studying a single viral protein and I would like to identify protein systems which are enriched in my sample compared to my negative control. I'm also interested in significantly enriched proteins but that isn't difficult to determine. What I'm most interested is in how I can apply what you've done in NeST to my own research question. It's not cancer related, however, the viral protein I'm looking at is oncogenic and the difference between viral infection and oncogenesis sometimes aren't that different. Computationally, could my two conditions (viral protein vs negative control) just be considered tumor vs non-tumor?