diff --git a/doc/source/admin/galaxy_options.rst b/doc/source/admin/galaxy_options.rst
index 8f4aef21d667..45386d72d861 100644
--- a/doc/source/admin/galaxy_options.rst
+++ b/doc/source/admin/galaxy_options.rst
@@ -5115,6 +5115,26 @@
 :Type: str
 
 
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
+``workflow_scheduling_separate_materialization_iteration``
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
+
+:Description:
+    Workflows launched with URI/URL inputs that are not marked as
+    'deferred' are "materialized" (or undeferred) by the workflow
+    scheduler. This might be a lengthy process. Setting this to 'True'
+    will place the invocation back in the queue after materialization
+    before scheduling the workflow so it is less  likely to starve
+    other workflow scheduling. Ideally, Galaxy would allow more fine
+    grain control of handlers but until then, this provides a way to
+    tip the balance between "doing more work" and "being more fair".
+    The default here is pretty arbitrary - it has been to False to
+    optimize Galaxy for automated, single user applications where
+    "fairness" is mostly irrelevant.
+:Default: ``false``
+:Type: bool
+
+
 ~~~~~~~~~~~~~~~~~~~~~~~~
 ``cache_user_job_count``
 ~~~~~~~~~~~~~~~~~~~~~~~~
@@ -5602,3 +5622,42 @@
     This requires the help_forum_api_url to be set.
 :Default: ``false``
 :Type: bool
+
+
+~~~~~~~~~~~~~~~~~~~~~~~~
+``file_source_temp_dir``
+~~~~~~~~~~~~~~~~~~~~~~~~
+
+:Description:
+    Directory to store temporary files for file sources. This defaults
+    to new_file_path if not set.
+:Default: ``None``
+:Type: str
+
+
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
+``file_source_webdav_use_temp_files``
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
+
+:Description:
+    Default value for use_temp_files for webdav plugins that don't
+    explicitly declare this.
+:Default: ``true``
+:Type: bool
+
+
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
+``file_source_listings_expiry_time``
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
+
+:Description:
+    Number of seconds before file source content listings are
+    refreshed. Shorter times will result in more queries while
+    browsing a file sources. Longer times will result in fewer
+    requests to file sources but outdated contents might be displayed
+    to the user. Currently only affects s3fs file sources.
+:Default: ``60``
+:Type: int
+
+
+
diff --git a/lib/galaxy/config/__init__.py b/lib/galaxy/config/__init__.py
index ecf8f8aebcfb..80d57ab04bf6 100644
--- a/lib/galaxy/config/__init__.py
+++ b/lib/galaxy/config/__init__.py
@@ -704,6 +704,7 @@ class GalaxyAppConfiguration(BaseAppConfiguration, CommonConfigurationMixin):
     drmaa_external_runjob_script: str
     email_from: Optional[str]
     enable_tool_shed_check: bool
+    file_source_temp_dir: str
     galaxy_data_manager_data_path: str
     galaxy_infrastructure_url: str
     hours_between_check: int
@@ -1236,6 +1237,9 @@ def _load_theme(path: str, theme_dict: dict):
         else:
             _load_theme(self.themes_config_file, self.themes)
 
+        if self.file_source_temp_dir:
+            self.file_source_temp_dir = os.path.abspath(self.file_source_temp_dir)
+
     def _process_celery_config(self):
         if self.celery_conf and self.celery_conf.get("result_backend") is None:
             # If the result_backend is not set, use a SQLite database in the data directory
@@ -1348,6 +1352,7 @@ def check(self):
             self.template_cache_path,
             self.tool_data_path,
             self.user_library_import_dir,
+            self.file_source_temp_dir,
         ]
         for path in paths_to_check:
             self._ensure_directory(path)
diff --git a/lib/galaxy/config/sample/galaxy.yml.sample b/lib/galaxy/config/sample/galaxy.yml.sample
index 7c3e326a0a0c..d6718cf176e7 100644
--- a/lib/galaxy/config/sample/galaxy.yml.sample
+++ b/lib/galaxy/config/sample/galaxy.yml.sample
@@ -2748,6 +2748,19 @@ galaxy:
   # <config_dir>.
   #workflow_schedulers_config_file: workflow_schedulers_conf.xml
 
+  # Workflows launched with URI/URL inputs that are not marked as
+  # 'deferred' are "materialized" (or undeferred) by the workflow
+  # scheduler. This might be a lengthy process. Setting this to 'True'
+  # will place the invocation back in the queue after materialization
+  # before scheduling the workflow so it is less  likely to starve other
+  # workflow scheduling. Ideally, Galaxy would allow more fine grain
+  # control of handlers but until then, this provides a way to tip the
+  # balance between "doing more work" and "being more fair". The default
+  # here is pretty arbitrary - it has been to False to optimize Galaxy
+  # for automated, single user applications where "fairness" is mostly
+  # irrelevant.
+  #workflow_scheduling_separate_materialization_iteration: false
+
   # If using job concurrency limits (configured in job_config_file),
   # several extra database queries must be performed to determine the
   # number of jobs a user has dispatched to a given destination.  By
@@ -2978,3 +2991,19 @@ galaxy:
   # Enable the integration of the Galaxy Help Forum in the tool panel.
   # This requires the help_forum_api_url to be set.
   #enable_help_forum_tool_panel_integration: false
+
+  # Directory to store temporary files for file sources. This defaults
+  # to new_file_path if not set.
+  #file_source_temp_dir: null
+
+  # Default value for use_temp_files for webdav plugins that don't
+  # explicitly declare this.
+  #file_source_webdav_use_temp_files: true
+
+  # Number of seconds before file source content listings are refreshed.
+  # Shorter times will result in more queries while browsing a file
+  # sources. Longer times will result in fewer requests to file sources
+  # but outdated contents might be displayed to the user. Currently only
+  # affects s3fs file sources.
+  #file_source_listings_expiry_time: 60
+
diff --git a/lib/galaxy/config/schemas/config_schema.yml b/lib/galaxy/config/schemas/config_schema.yml
index afcb6ec9c3ed..4b8519a6624f 100644
--- a/lib/galaxy/config/schemas/config_schema.yml
+++ b/lib/galaxy/config/schemas/config_schema.yml
@@ -4104,3 +4104,23 @@ mapping:
         per_host: true
         desc: |
           Enable the integration of the Galaxy Help Forum in the tool panel. This requires the help_forum_api_url to be set.
+
+      file_source_temp_dir:
+        type: str
+        required: false
+        desc: |
+          Directory to store temporary files for file sources. This defaults to new_file_path if not set.
+      
+      file_source_webdav_use_temp_files:
+        type: bool
+        default: true
+        desc: |
+          Default value for use_temp_files for webdav plugins that don't explicitly declare this.
+
+      file_source_listings_expiry_time:
+        type: int
+        default: 60
+        desc: |
+          Number of seconds before file source content listings are refreshed. Shorter times will result in more
+          queries while browsing a file sources. Longer times will result in fewer requests to file sources but
+          outdated contents might be displayed to the user. Currently only affects s3fs file sources.
diff --git a/lib/galaxy/files/plugins.py b/lib/galaxy/files/plugins.py
index 4f9c182f846a..fcbee3fdeaf8 100644
--- a/lib/galaxy/files/plugins.py
+++ b/lib/galaxy/files/plugins.py
@@ -24,6 +24,9 @@ class FileSourcePluginsConfig:
     user_library_import_dir: Optional[str]
     ftp_upload_dir: Optional[str]
     ftp_upload_purge: bool
+    tmp_dir: Optional[str]
+    webdav_use_temp_files: Optional[bool]
+    listings_expiry_time: Optional[int]
 
     def __init__(
         self,
@@ -33,6 +36,9 @@ def __init__(
         user_library_import_dir=None,
         ftp_upload_dir=None,
         ftp_upload_purge=True,
+        tmp_dir=None,
+        webdav_use_temp_files=None,
+        listings_expiry_time=None,
     ):
         symlink_allowlist = symlink_allowlist or []
         fetch_url_allowlist = fetch_url_allowlist or []
@@ -42,6 +48,9 @@ def __init__(
         self.user_library_import_dir = user_library_import_dir
         self.ftp_upload_dir = ftp_upload_dir
         self.ftp_upload_purge = ftp_upload_purge
+        self.tmp_dir = tmp_dir
+        self.webdav_use_temp_files = webdav_use_temp_files
+        self.listings_expiry_time = listings_expiry_time
 
     @staticmethod
     def from_app_config(config):
@@ -54,6 +63,10 @@ def from_app_config(config):
         kwds["user_library_import_dir"] = config.user_library_import_dir
         kwds["ftp_upload_dir"] = config.ftp_upload_dir
         kwds["ftp_upload_purge"] = config.ftp_upload_purge
+        kwds["tmp_dir"] = config.file_source_temp_dir
+        kwds["webdav_use_temp_files"] = config.file_source_webdav_use_temp_files
+        kwds["listings_expiry_time"] = config.file_source_listings_expiry_time
+
         return FileSourcePluginsConfig(**kwds)
 
     def to_dict(self):
@@ -64,6 +77,9 @@ def to_dict(self):
             "user_library_import_dir": self.user_library_import_dir,
             "ftp_upload_dir": self.ftp_upload_dir,
             "ftp_upload_purge": self.ftp_upload_purge,
+            "tmp_dir": self.tmp_dir,
+            "webdav_use_temp_files": self.webdav_use_temp_files,
+            "listings_expiry_time": self.listings_expiry_time,
         }
 
     @staticmethod
@@ -75,6 +91,10 @@ def from_dict(as_dict):
             user_library_import_dir=as_dict["user_library_import_dir"],
             ftp_upload_dir=as_dict["ftp_upload_dir"],
             ftp_upload_purge=as_dict["ftp_upload_purge"],
+            # Always provided for new jobs, remove in 25.0
+            tmp_dir=as_dict.get("tmp_dir"),
+            webdav_use_temp_files=as_dict.get("webdav_use_temp_files"),
+            listings_expiry_time=as_dict.get("listings_expiry_time"),
         )
 
 
diff --git a/lib/galaxy/files/sources/_pyfilesystem2.py b/lib/galaxy/files/sources/_pyfilesystem2.py
index afcff2884961..5b3add6e6119 100644
--- a/lib/galaxy/files/sources/_pyfilesystem2.py
+++ b/lib/galaxy/files/sources/_pyfilesystem2.py
@@ -38,6 +38,7 @@ class PyFilesystem2FilesSource(BaseFilesSource):
     required_package: ClassVar[str]
     supports_pagination = True
     supports_search = True
+    allow_key_error_on_empty_directories = False  # work around a bug in webdav
 
     def __init__(self, **kwd: Unpack[FilesSourceProperties]):
         if self.required_module is None:
@@ -65,10 +66,14 @@ def _list(
             with self._open_fs(user_context=user_context, opts=opts) as h:
                 if recursive:
                     recursive_result: List[AnyRemoteEntry] = []
-                    for p, dirs, files in h.walk(path, namespaces=["details"]):
-                        to_dict = functools.partial(self._resource_info_to_dict, p)
-                        recursive_result.extend(map(to_dict, dirs))
-                        recursive_result.extend(map(to_dict, files))
+                    try:
+                        for p, dirs, files in h.walk(path, namespaces=["details"]):
+                            to_dict = functools.partial(self._resource_info_to_dict, p)
+                            recursive_result.extend(map(to_dict, dirs))
+                            recursive_result.extend(map(to_dict, files))
+                    except KeyError:
+                        if not self.allow_key_error_on_empty_directories:
+                            raise
                     return recursive_result, len(recursive_result)
                 else:
                     page = self._to_page(limit, offset)
diff --git a/lib/galaxy/files/sources/s3fs.py b/lib/galaxy/files/sources/s3fs.py
index 6967f4a5b6a7..c6a7dedc96d3 100644
--- a/lib/galaxy/files/sources/s3fs.py
+++ b/lib/galaxy/files/sources/s3fs.py
@@ -8,7 +8,10 @@
     Tuple,
 )
 
-from typing_extensions import Unpack
+from typing_extensions import (
+    NotRequired,
+    Unpack,
+)
 
 from galaxy.files import OptionalUserContext
 from . import (
@@ -35,6 +38,7 @@ class S3FsFilesSourceProperties(FilesSourceProperties, total=False):
     endpoint_url: int
     user: str
     passwd: str
+    listings_expiry_time: NotRequired[Optional[int]]
     client_kwargs: dict  # internally computed. Should not be specified in config file
 
 
@@ -45,6 +49,14 @@ def __init__(self, **kwd: Unpack[S3FsFilesSourceProperties]):
         if s3fs is None:
             raise Exception("Package s3fs unavailable but required for this file source plugin.")
         props: S3FsFilesSourceProperties = cast(S3FsFilesSourceProperties, self._parse_common_config_opts(kwd))
+        file_sources_config = self._file_sources_config
+        if (
+            props.get("listings_expiry_time") is None
+            and file_sources_config
+            and file_sources_config.listings_expiry_time
+        ):
+            if file_sources_config.listings_expiry_time:
+                props["listings_expiry_time"] = file_sources_config.listings_expiry_time
         # There is a possibility that the bucket name could be parameterized: e.g.
         # bucket: ${user.preferences['generic_s3|bucket']}
         # that's ok, because we evaluate the bucket name again later. The bucket property here will only
diff --git a/lib/galaxy/files/sources/webdav.py b/lib/galaxy/files/sources/webdav.py
index 2e11a51d98b2..9aec17fdbdc4 100644
--- a/lib/galaxy/files/sources/webdav.py
+++ b/lib/galaxy/files/sources/webdav.py
@@ -5,10 +5,13 @@
 
 import tempfile
 from typing import (
+    cast,
     Optional,
     Union,
 )
 
+from typing_extensions import NotRequired
+
 from . import (
     FilesSourceOptions,
     FilesSourceProperties,
@@ -16,18 +19,35 @@
 from ._pyfilesystem2 import PyFilesystem2FilesSource
 
 
+class WebDavFilesSourceProperties(FilesSourceProperties, total=False):
+    use_temp_files: NotRequired[Optional[bool]]
+    temp_path: NotRequired[Optional[str]]
+
+
 class WebDavFilesSource(PyFilesystem2FilesSource):
     plugin_type = "webdav"
     required_module = WebDAVFS
     required_package = "fs.webdavfs"
+    allow_key_error_on_empty_directories = True
 
     def _open_fs(self, user_context=None, opts: Optional[FilesSourceOptions] = None):
-        props = self._serialization_props(user_context)
+        props = cast(WebDavFilesSourceProperties, self._serialization_props(user_context))
+        file_sources_config = self._file_sources_config
         use_temp_files = props.pop("use_temp_files", None)
+        if use_temp_files is None and file_sources_config and file_sources_config.webdav_use_temp_files is not None:
+            use_temp_files = file_sources_config.webdav_use_temp_files
         if use_temp_files is None:
             # Default to True to avoid memory issues with large files.
-            props["use_temp_files"] = True
-            props["temp_path"] = props.get("temp_path", tempfile.TemporaryDirectory(prefix="webdav_"))
+            use_temp_files = True
+
+        if use_temp_files:
+            temp_path = props.get("temp_path")
+            if temp_path is None and file_sources_config and file_sources_config.tmp_dir:
+                temp_path = file_sources_config.tmp_dir
+            if temp_path is None:
+                temp_path = tempfile.mkdtemp(prefix="webdav_")
+            props["temp_path"] = temp_path
+        props["use_temp_files"] = use_temp_files
         extra_props: Union[FilesSourceProperties, dict] = opts.extra_props or {} if opts else {}
         handle = WebDAVFS(**{**props, **extra_props})
         return handle
diff --git a/lib/galaxy/model/unittest_utils/data_app.py b/lib/galaxy/model/unittest_utils/data_app.py
index 75e5101eca80..0d424cca3623 100644
--- a/lib/galaxy/model/unittest_utils/data_app.py
+++ b/lib/galaxy/model/unittest_utils/data_app.py
@@ -79,6 +79,10 @@ def __init__(self, root=None, **kwd):
         self.ftp_upload_dir = None
         self.ftp_upload_purge = False
 
+        self.file_source_temp_dir = None
+        self.file_source_webdav_use_temp_files = False
+        self.file_source_listings_expiry_time = 60
+
     def __del__(self):
         if self._remove_root:
             shutil.rmtree(self.root)
diff --git a/test/unit/files/_util.py b/test/unit/files/_util.py
index 16ad933affa4..2bf1274fcee5 100644
--- a/test/unit/files/_util.py
+++ b/test/unit/files/_util.py
@@ -2,6 +2,7 @@
 
 import os
 import tempfile
+from typing import Optional
 
 from galaxy.files import (
     ConfiguredFileSources,
@@ -156,8 +157,9 @@ def write_from(
         file_source_path.file_source.write_from(file_source_path.path, f.name, user_context=user_context)
 
 
-def configured_file_sources(conf_file):
-    file_sources_config = FileSourcePluginsConfig()
+def configured_file_sources(conf_file, file_sources_config: Optional[FileSourcePluginsConfig] = None):
+    file_sources_config = file_sources_config or FileSourcePluginsConfig()
+    assert file_sources_config
     if isinstance(conf_file, str):
         conf = ConfiguredFileSourcesConf(conf_file=conf_file)
     else:
diff --git a/test/unit/files/test_webdav.py b/test/unit/files/test_webdav.py
index 071281e1bc88..9294881ec247 100644
--- a/test/unit/files/test_webdav.py
+++ b/test/unit/files/test_webdav.py
@@ -1,9 +1,10 @@
 # docker run -v `pwd`/test/integration/webdav/data:/media  -e WEBDAV_USERNAME=alice -e WEBDAV_PASSWORD=secret1234 -p 7083:7083 jmchilton/webdavdev
-# GALAXY_TEST_WEBDAV=1 pytest test/unit/files/webdav.py
+# GALAXY_TEST_WEBDAV=1 pytest test/unit/files/test_webdav.py
 import os
 
 import pytest
 
+from galaxy.files.plugins import FileSourcePluginsConfig
 from ._util import (
     configured_file_sources,
     find,
@@ -17,6 +18,7 @@
 
 SCRIPT_DIRECTORY = os.path.abspath(os.path.dirname(__file__))
 FILE_SOURCES_CONF = os.path.join(SCRIPT_DIRECTORY, "webdav_file_sources_conf.yml")
+FILE_SOURCES_CONF_NO_USE_TEMP_FILES = os.path.join(SCRIPT_DIRECTORY, "webdav_file_sources_without_use_temp_conf.yml")
 USER_FILE_SOURCES_CONF = os.path.join(SCRIPT_DIRECTORY, "webdav_user_file_sources_conf.yml")
 
 skip_if_no_webdav = pytest.mark.skipif(not os.environ.get("GALAXY_TEST_WEBDAV"), reason="GALAXY_TEST_WEBDAV not set")
@@ -62,17 +64,40 @@ def test_sniff_to_tmp():
 
 @skip_if_no_webdav
 def test_serialization():
-    # serialize the configured file sources and rematerialize them,
-    # ensure they still function. This is needed for uploading files.
-    file_sources = serialize_and_recover(configured_file_sources(FILE_SOURCES_CONF))
+    configs = [FILE_SOURCES_CONF_NO_USE_TEMP_FILES, FILE_SOURCES_CONF]
+    for config in configs:
+        # serialize the configured file sources and rematerialize them,
+        # ensure they still function. This is needed for uploading files.
+        file_sources = serialize_and_recover(configured_file_sources(config))
 
-    res = list_root(file_sources, "gxfiles://test1", recursive=True)
-    assert find_file_a(res)
+        res = list_root(file_sources, "gxfiles://test1", recursive=True)
+        assert find_file_a(res)
 
-    res = list_root(file_sources, "gxfiles://test1", recursive=False)
-    assert find_file_a(res)
+        res = list_root(file_sources, "gxfiles://test1", recursive=False)
+        assert find_file_a(res)
 
-    _download_and_check_file(file_sources)
+        _download_and_check_file(file_sources)
+
+
+@skip_if_no_webdav
+def test_config_options():
+    file_sources = configured_file_sources(FILE_SOURCES_CONF)
+    fs = file_sources._file_sources[0]
+    user_context = user_context_fixture()
+    assert fs._open_fs(user_context).use_temp_files
+
+    file_sources = configured_file_sources(FILE_SOURCES_CONF_NO_USE_TEMP_FILES)
+    fs = file_sources._file_sources[0]
+    user_context = user_context_fixture()
+    assert not fs._open_fs(user_context).use_temp_files
+
+    disable_default_use_temp = FileSourcePluginsConfig(
+        webdav_use_temp_files=False,
+    )
+    file_sources = configured_file_sources(FILE_SOURCES_CONF, disable_default_use_temp)
+    fs = file_sources._file_sources[0]
+    user_context = user_context_fixture()
+    assert not fs._open_fs(user_context).use_temp_files
 
 
 @skip_if_no_webdav
diff --git a/test/unit/files/webdav_file_sources_conf.yml b/test/unit/files/webdav_file_sources_conf.yml
new file mode 100644
index 000000000000..6116edd6de1f
--- /dev/null
+++ b/test/unit/files/webdav_file_sources_conf.yml
@@ -0,0 +1,6 @@
+- type: webdav
+  id: test1
+  doc: Test WebDAV server.
+  url: http://127.0.0.1:7083
+  login: alice
+  password: secret1234  
diff --git a/test/unit/files/webdav_file_sources_without_use_temp_conf.yml b/test/unit/files/webdav_file_sources_without_use_temp_conf.yml
new file mode 100644
index 000000000000..916f8068f459
--- /dev/null
+++ b/test/unit/files/webdav_file_sources_without_use_temp_conf.yml
@@ -0,0 +1,7 @@
+- type: webdav
+  id: test1
+  doc: Test WebDAV server.
+  url: http://127.0.0.1:7083
+  login: alice
+  password: secret1234  
+  use_temp_files: false