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@@ -7,13 +7,28 @@ mtDNA-Server 2 is a Nextflow DSL2 pipeline to accurately detect heteroplasmic an | |
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 | ||
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## Publication | ||
Weissensteiner H*, Forer L*, Kronenberg F, Schönherr S. [mtDNA-Server 2: advancing mitochondrial DNA analysis through highly parallelized data processing and interactive analytics](https://doi.org/10.1093/nar/gkae296). Nucleic Acids Res. 2024 May 6:gkae296. doi: 10.1093/nar/gkae296. Epub ahead of print. PMID: 38709886. | ||
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## Version History | ||
Release [v2.1.11](../../releases/tag/v2.1.11) - Improve QC command, update to latest mutserve v2.0.1. | ||
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Release [v2.1.10](../../releases/tag/v2.1.10) - Improved mutect2 support: create missing RG tags, write inidividual reference sequence on the fly, support complex ref tags. | ||
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### Are your files not working? | ||
We want to steadily improve mtDNA-Server 2. If your files are not working, please feel free to create a issue or contact [[Sebastian](mailto:[email protected])] directly. | ||
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## How to execute mtDNA-Server 2? | ||
mtDNA-Server 2 is available as a **hosted web-service** at the Medical University of Innsbruck or as a **Nextflow pipeline**. | ||
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## Web Service | ||
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mtDNA-Server 2 is hosted as a **free** service on our [mitoverse](https://mitoverse.i-med.ac.at/) platform. | ||
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### Documentation | ||
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The web-service documentation can be accessed [here](https://mitoverse.readthedocs.io/mtdna-server/mtdna-server/). | ||
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## Nextflow Pipeline | ||
To run mtDNA-Server 2 via Nextflow, please execute the following steps. | ||
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@@ -31,32 +46,7 @@ To run mtDNA-Server 2 on your own data, create a [config file](tests/test_mitohp | |
nextflow run genepi/mtdna-server-2 -r v2.1.11 -c <your-config-file> -profile docker | ||
``` | ||
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## Documentation | ||
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Documentation can be accessed [here](https://mitoverse.readthedocs.io/mtdna-server/mtdna-server/). | ||
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 | ||
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## Publication | ||
Weissensteiner H*, Forer L*, Kronenberg F, Schönherr S. [mtDNA-Server 2: advancing mitochondrial DNA analysis through highly parallelized data processing and interactive analytics](https://doi.org/10.1093/nar/gkae296). Nucleic Acids Res. 2024 May 6:gkae296. doi: 10.1093/nar/gkae296. Epub ahead of print. PMID: 38709886. | ||
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### Version History | ||
Release [v2.1.11](../../releases/tag/v2.1.11) - Improve QC command, update to latest mutserve v2.0.1. | ||
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||
Release [v2.1.10](../../releases/tag/v2.1.10) - Improved mutect2 support: create missing RG tags, write inidividual reference sequence on the fly, support complex ref tags. | ||
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## Are your files not working? | ||
We want to steadily improve mtDNA-Server 2. If your files are not working, please feel free to create a issue or contact [[Sebastian](mailto:[email protected])] directly. | ||
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### Development | ||
``` | ||
git clone https://github.com/genepi/mtdna-server-2 | ||
cd mtdna-server-2 | ||
docker build -t genepi/mtdna-server-2 . # don't ignore the dot | ||
nextflow run main.nf -profile test,development | ||
``` | ||
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### Documentation | ||
### Parameters | ||
The following parameters can be set in the configuration file. | ||
| Parameter | Default Value | Comment | | ||
|----------------------|--------------|----------------| | ||
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@@ -72,6 +62,16 @@ The following parameters can be set in the configuration file. | |
| alignQ | 30 | Alignment quality threshold | | ||
| output | null | Specific Output folder | | ||
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## Development | ||
``` | ||
git clone https://github.com/genepi/mtdna-server-2 | ||
cd mtdna-server-2 | ||
docker build -t genepi/mtdna-server-2 . # don't ignore the dot | ||
nextflow run main.nf -profile test,development | ||
``` | ||
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## Contact | ||
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