diff --git a/dev-packages/node-integration-tests/package.json b/dev-packages/node-integration-tests/package.json
index 9243a1512884..0ac7fdbf0aa2 100644
--- a/dev-packages/node-integration-tests/package.json
+++ b/dev-packages/node-integration-tests/package.json
@@ -44,6 +44,7 @@
     "cors": "^2.8.5",
     "cron": "^3.1.6",
     "express": "^4.17.3",
+    "generic-pool": "^3.9.0",
     "graphql": "^16.3.0",
     "http-terminator": "^3.2.0",
     "ioredis": "^5.4.1",
diff --git a/dev-packages/node-integration-tests/suites/tracing/genericPool/scenario.js b/dev-packages/node-integration-tests/suites/tracing/genericPool/scenario.js
new file mode 100644
index 000000000000..74d5f73693f5
--- /dev/null
+++ b/dev-packages/node-integration-tests/suites/tracing/genericPool/scenario.js
@@ -0,0 +1,71 @@
+const { loggingTransport } = require('@sentry-internal/node-integration-tests');
+const Sentry = require('@sentry/node');
+
+Sentry.init({
+  dsn: 'https://public@dsn.ingest.sentry.io/1337',
+  release: '1.0',
+  tracesSampleRate: 1.0,
+  transport: loggingTransport,
+});
+
+// Stop the process from exiting before the transaction is sent
+setInterval(() => {}, 1000);
+
+const mysql = require('mysql');
+const genericPool = require('generic-pool');
+
+const factory = {
+  create: function () {
+    return mysql.createConnection({
+      user: 'root',
+      password: 'docker',
+    });
+  },
+  destroy: function (client) {
+    client.end(err => {
+      if (err) {
+        // eslint-disable-next-line no-console
+        console.error('Error while disconnecting MySQL:', err);
+      }
+    });
+  },
+};
+
+const opts = {
+  max: 10,
+  min: 2,
+};
+
+const myPool = genericPool.createPool(factory, opts);
+
+async function run() {
+  await Sentry.startSpan(
+    {
+      op: 'transaction',
+      name: 'Test Transaction',
+    },
+    async () => {
+      try {
+        const client1 = await myPool.acquire();
+        const client2 = await myPool.acquire();
+
+        client1.query('SELECT NOW()', function () {
+          myPool.release(client1);
+        });
+
+        client2.query('SELECT 1 + 1 AS solution', function () {
+          myPool.release(client2);
+        });
+      } catch (err) {
+        // eslint-disable-next-line no-console
+        console.error('Error while pooling MySQL:', err);
+      } finally {
+        await myPool.drain();
+        await myPool.clear();
+      }
+    },
+  );
+}
+
+// eslint-disable-next-line @typescript-eslint/no-floating-promises
+run();
diff --git a/dev-packages/node-integration-tests/suites/tracing/genericPool/test.ts b/dev-packages/node-integration-tests/suites/tracing/genericPool/test.ts
new file mode 100644
index 000000000000..a61782ae0fe5
--- /dev/null
+++ b/dev-packages/node-integration-tests/suites/tracing/genericPool/test.ts
@@ -0,0 +1,34 @@
+import { cleanupChildProcesses, createRunner } from '../../../utils/runner';
+
+describe('genericPool auto instrumentation', () => {
+  afterAll(() => {
+    cleanupChildProcesses();
+  });
+
+  test('should auto-instrument `genericPool` package when calling pool.require()', done => {
+    const EXPECTED_TRANSACTION = {
+      transaction: 'Test Transaction',
+      spans: expect.arrayContaining([
+        expect.objectContaining({
+          description: expect.stringMatching(/^generic-pool\.ac?quire/),
+          origin: 'auto.db.otel.generic-pool',
+          data: {
+            'sentry.origin': 'auto.db.otel.generic-pool',
+          },
+          status: 'ok',
+        }),
+
+        expect.objectContaining({
+          description: expect.stringMatching(/^generic-pool\.ac?quire/),
+          origin: 'auto.db.otel.generic-pool',
+          data: {
+            'sentry.origin': 'auto.db.otel.generic-pool',
+          },
+          status: 'ok',
+        }),
+      ]),
+    };
+
+    createRunner(__dirname, 'scenario.js').expect({ transaction: EXPECTED_TRANSACTION }).start(done);
+  });
+});
diff --git a/packages/astro/src/index.server.ts b/packages/astro/src/index.server.ts
index 0895eb86e364..747870da3014 100644
--- a/packages/astro/src/index.server.ts
+++ b/packages/astro/src/index.server.ts
@@ -43,6 +43,7 @@ export {
   fsIntegration,
   functionToStringIntegration,
   generateInstrumentOnce,
+  genericPoolIntegration,
   getActiveSpan,
   getAutoPerformanceIntegrations,
   getClient,
diff --git a/packages/aws-serverless/src/index.ts b/packages/aws-serverless/src/index.ts
index 27f48c2da0db..f648dba045ec 100644
--- a/packages/aws-serverless/src/index.ts
+++ b/packages/aws-serverless/src/index.ts
@@ -87,6 +87,7 @@ export {
   setupConnectErrorHandler,
   fastifyIntegration,
   fsIntegration,
+  genericPoolIntegration,
   graphqlIntegration,
   mongoIntegration,
   mongooseIntegration,
diff --git a/packages/bun/src/index.ts b/packages/bun/src/index.ts
index d4ca06f85584..1d8b02c33568 100644
--- a/packages/bun/src/index.ts
+++ b/packages/bun/src/index.ts
@@ -108,6 +108,7 @@ export {
   setupKoaErrorHandler,
   connectIntegration,
   setupConnectErrorHandler,
+  genericPoolIntegration,
   graphqlIntegration,
   mongoIntegration,
   mongooseIntegration,
diff --git a/packages/google-cloud-serverless/src/index.ts b/packages/google-cloud-serverless/src/index.ts
index f58305c64f6c..463a0c5c1246 100644
--- a/packages/google-cloud-serverless/src/index.ts
+++ b/packages/google-cloud-serverless/src/index.ts
@@ -87,6 +87,7 @@ export {
   connectIntegration,
   setupConnectErrorHandler,
   fastifyIntegration,
+  genericPoolIntegration,
   graphqlIntegration,
   mongoIntegration,
   mongooseIntegration,
diff --git a/packages/node/package.json b/packages/node/package.json
index f17c37541f63..6a64f646f64c 100644
--- a/packages/node/package.json
+++ b/packages/node/package.json
@@ -73,6 +73,7 @@
     "@opentelemetry/instrumentation-express": "0.41.1",
     "@opentelemetry/instrumentation-fastify": "0.38.0",
     "@opentelemetry/instrumentation-fs": "0.14.0",
+    "@opentelemetry/instrumentation-generic-pool": "0.38.0",
     "@opentelemetry/instrumentation-graphql": "0.42.0",
     "@opentelemetry/instrumentation-hapi": "0.40.0",
     "@opentelemetry/instrumentation-http": "0.52.1",
diff --git a/packages/node/src/index.ts b/packages/node/src/index.ts
index 5f4adf315fd5..6ce3c325e3ff 100644
--- a/packages/node/src/index.ts
+++ b/packages/node/src/index.ts
@@ -26,6 +26,7 @@ export { hapiIntegration, setupHapiErrorHandler } from './integrations/tracing/h
 export { koaIntegration, setupKoaErrorHandler } from './integrations/tracing/koa';
 export { connectIntegration, setupConnectErrorHandler } from './integrations/tracing/connect';
 export { spotlightIntegration } from './integrations/spotlight';
+export { genericPoolIntegration } from './integrations/tracing/genericPool';
 
 export { SentryContextManager } from './otel/contextManager';
 export { generateInstrumentOnce } from './otel/instrument';
diff --git a/packages/node/src/integrations/tracing/genericPool.ts b/packages/node/src/integrations/tracing/genericPool.ts
new file mode 100644
index 000000000000..55b8b6095f64
--- /dev/null
+++ b/packages/node/src/integrations/tracing/genericPool.ts
@@ -0,0 +1,40 @@
+import { GenericPoolInstrumentation } from '@opentelemetry/instrumentation-generic-pool';
+import { SEMANTIC_ATTRIBUTE_SENTRY_ORIGIN, defineIntegration, spanToJSON } from '@sentry/core';
+import type { IntegrationFn } from '@sentry/types';
+import { generateInstrumentOnce } from '../../otel/instrument';
+
+const INTEGRATION_NAME = 'GenericPool';
+
+export const instrumentGenericPool = generateInstrumentOnce(INTEGRATION_NAME, () => new GenericPoolInstrumentation({}));
+
+const _genericPoolIntegration = (() => {
+  return {
+    name: INTEGRATION_NAME,
+    setupOnce() {
+      instrumentGenericPool();
+    },
+
+    setup(client) {
+      client.on('spanStart', span => {
+        const spanJSON = spanToJSON(span);
+
+        const spanDescription = spanJSON.description;
+
+        // typo in emitted span for version <= 0.38.0 of @opentelemetry/instrumentation-generic-pool
+        const isGenericPoolSpan =
+          spanDescription === 'generic-pool.aquire' || spanDescription === 'generic-pool.acquire';
+
+        if (isGenericPoolSpan) {
+          span.setAttribute(SEMANTIC_ATTRIBUTE_SENTRY_ORIGIN, 'auto.db.otel.generic-pool');
+        }
+      });
+    },
+  };
+}) satisfies IntegrationFn;
+
+/**
+ * GenericPool integration
+ *
+ * Capture tracing data for GenericPool.
+ */
+export const genericPoolIntegration = defineIntegration(_genericPoolIntegration);
diff --git a/packages/node/src/integrations/tracing/index.ts b/packages/node/src/integrations/tracing/index.ts
index 886c11683674..46a9f79e4caa 100644
--- a/packages/node/src/integrations/tracing/index.ts
+++ b/packages/node/src/integrations/tracing/index.ts
@@ -4,6 +4,7 @@ import { instrumentHttp } from '../http';
 import { connectIntegration, instrumentConnect } from './connect';
 import { expressIntegration, instrumentExpress } from './express';
 import { fastifyIntegration, instrumentFastify } from './fastify';
+import { genericPoolIntegration, instrumentGenericPool } from './genericPool';
 import { graphqlIntegration, instrumentGraphql } from './graphql';
 import { hapiIntegration, instrumentHapi } from './hapi';
 import { instrumentKoa, koaIntegration } from './koa';
@@ -37,6 +38,7 @@ export function getAutoPerformanceIntegrations(): Integration[] {
     hapiIntegration(),
     koaIntegration(),
     connectIntegration(),
+    genericPoolIntegration(),
   ];
 }
 
@@ -61,5 +63,6 @@ export function getOpenTelemetryInstrumentationToPreload(): (((options?: any) =>
     instrumentHapi,
     instrumentGraphql,
     instrumentRedis,
+    instrumentGenericPool,
   ];
 }
diff --git a/packages/remix/src/index.server.ts b/packages/remix/src/index.server.ts
index 7ab6efb15827..457dcb9f8685 100644
--- a/packages/remix/src/index.server.ts
+++ b/packages/remix/src/index.server.ts
@@ -47,6 +47,7 @@ export {
   flush,
   functionToStringIntegration,
   generateInstrumentOnce,
+  genericPoolIntegration,
   getActiveSpan,
   getAutoPerformanceIntegrations,
   getClient,
diff --git a/packages/solidstart/src/server/index.ts b/packages/solidstart/src/server/index.ts
index c3499a82459a..995f58d057e3 100644
--- a/packages/solidstart/src/server/index.ts
+++ b/packages/solidstart/src/server/index.ts
@@ -38,6 +38,7 @@ export {
   flush,
   functionToStringIntegration,
   generateInstrumentOnce,
+  genericPoolIntegration,
   getActiveSpan,
   getAutoPerformanceIntegrations,
   getClient,
diff --git a/packages/sveltekit/src/server/index.ts b/packages/sveltekit/src/server/index.ts
index 32dd6627d7a6..d57ec35bd7cc 100644
--- a/packages/sveltekit/src/server/index.ts
+++ b/packages/sveltekit/src/server/index.ts
@@ -37,6 +37,7 @@ export {
   fastifyIntegration,
   flush,
   functionToStringIntegration,
+  genericPoolIntegration,
   generateInstrumentOnce,
   getActiveSpan,
   getAutoPerformanceIntegrations,
diff --git a/yarn.lock b/yarn.lock
index 6f2432d7054a..1acd7e11fbd9 100644
--- a/yarn.lock
+++ b/yarn.lock
@@ -7113,6 +7113,13 @@
     "@opentelemetry/core" "^1.8.0"
     "@opentelemetry/instrumentation" "^0.52.0"
 
+"@opentelemetry/instrumentation-generic-pool@0.38.0":
+  version "0.38.0"
+  resolved "https://registry.yarnpkg.com/@opentelemetry/instrumentation-generic-pool/-/instrumentation-generic-pool-0.38.0.tgz#9ea4d82da23541cda613d553bd405b2cbc0da184"
+  integrity sha512-0/ULi6pIco1fEnDPmmAul8ZoudFL7St0hjgBbWZlZPBCSyslDll1J7DFeEbjiRSSyUd+0tu73ae0DOKVKNd7VA==
+  dependencies:
+    "@opentelemetry/instrumentation" "^0.52.0"
+
 "@opentelemetry/instrumentation-graphql@0.42.0":
   version "0.42.0"
   resolved "https://registry.yarnpkg.com/@opentelemetry/instrumentation-graphql/-/instrumentation-graphql-0.42.0.tgz#588a18c39e3b3f655bc09243566172ab0b638d35"
@@ -19084,7 +19091,7 @@ generate-function@^2.3.1:
   dependencies:
     is-property "^1.0.2"
 
-generic-pool@3.9.0:
+generic-pool@3.9.0, generic-pool@^3.9.0:
   version "3.9.0"
   resolved "https://registry.yarnpkg.com/generic-pool/-/generic-pool-3.9.0.tgz#36f4a678e963f4fdb8707eab050823abc4e8f5e4"
   integrity sha512-hymDOu5B53XvN4QT9dBmZxPX4CWhBPPLguTZ9MMFeFa/Kg0xWVfylOVNlJji/E7yTZWFd/q9GO5TxDLq156D7g==