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Missing tmap and refmap files in query gff directory + missing statistics in stats file #72

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jchang97 opened this issue Apr 20, 2022 · 0 comments

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@jchang97
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jchang97 commented Apr 20, 2022

Hi there,

Thank you for creating this wonderful tool!

I currently have two issues when running with gffcompare v0.12.2:

  1. I understand that the tmap and refmap files should be in the query gff directory, but upon running the code, I find that there are no files in the directory or any other relevant directories. Could you please help me understand what is going wrong?

I ran this code:

gffcompare -r /data/gpfs/projects/punim1466/db/refdata-gex-GRCh38-2020-A/genes/genes.gtf -i "/data/gpfs/projects/punim1466/analysis/nanopore/isoform/novel_isoforms/gff.txt"

And in 'gff.txt' I have the following:

/data/gpfs/projects/punim1466/analysis/nanopore/isoform/novel_isoforms/gff/libA/isoform_annotated.filtered_libA.gff3
/data/gpfs/projects/punim1466/analysis/nanopore/isoform/novel_isoforms/gff/libB/isoform_annotated.filtered_libB.gff3
/data/gpfs/projects/punim1466/analysis/nanopore/isoform/novel_isoforms/gff/libC/isoform_annotated.filtered_libC.gff3
/data/gpfs/projects/punim1466/analysis/nanopore/isoform/novel_isoforms/gff/libD/isoform_annotated.filtered_libD.gff3
/data/gpfs/projects/punim1466/analysis/nanopore/isoform/novel_isoforms/gff/libE/isoform_annotated.filtered_libE.gff3
/data/gpfs/projects/punim1466/analysis/nanopore/isoform/novel_isoforms/gff/libF/isoform_annotated.filtered_libF.gff3
/data/gpfs/projects/punim1466/analysis/nanopore/isoform/novel_isoforms/gff/libG/isoform_annotated.filtered_libG.gff3
/data/gpfs/projects/punim1466/analysis/nanopore/isoform/novel_isoforms/gff/libH/isoform_annotated.filtered_libH.gff3
  1. The output in my stats file are missing the percentages and only show:

# gffcompare v0.12.2 | Command line was:
#gffcompare -r /data/gpfs/projects/punim1466/db/refdata-gex-GRCh38-2020-A/genes/genes.gtf -i /data/gpfs/projects/punim1466/analysis/nanopore/isoform/novel_isoforms/gff.txt
#

 Total union super-loci across all input datasets: 14959 
  (12405 multi-transcript, ~8.4 transcripts per locus)
125784 out of 125784 consensus transcripts written in gffcmp.combined.gtf (0 discarded as redundant)

I do get the loci, stats, tracking and combined.gtf files.

Any help on these two issues would be greatly appreciated!

Thank you

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