Merging results from two lotus2 runs

[Amanda-Biocortex]

Hi,

I have data from 3 projects that I would like to compare. The methods were very similar but error rate liley vary, hence I would like to run them through lotus2 (particularly the error rate calculation of DADA2) separately.

Is there a way of running the 3 project separately through lotus2-DADA2 and lotus2-Lambda, and then combining the results at the end?
If I was using DADA2 in R I would use mergeSequenceTables, is there something similar I can do in lotus2 so that I can analyse all the data together?

[hildebra]

Hey Amanda,
please see #73
in short: I would recommend to

1. run all three data together if you can - take advantage of the "SequencingRun" column header in the lotus2 map
2. if not possible, combine at species level
3. if preferred, use the mergeSeqTable (which means literally searching for 100% identical nt sequences, i.e. you can use R and put the ASV seq as "name" of a row in the aundance matrix, and then merge this way)

hth,
Falk