Same ASV assigned to different taxonomy in two samples

[Amanda-Biocortex]

Hi,

Im looking to get consistent results per samples such that sample X in cohort A will have the same results as the same sample X in cohort B.

I have previously run dada2 (stand alone) on cohort A and cohort B and am now running the results through lotus2 lambda -taxOnly. Ive pulled the ASV taxonomy assignment for sample X in both cohorts and while the identified ASVs and counts are the same, the taxonomy results are different.

Do you know why the taxonomy of ASVs in sample X change dependent on which cohort the sample is in?

Apologies is this amounts to me not understanding lambda sufficiently!

[hildebra]

Hey,
it seems a bit weird, since the tax should be independent of cohort. I suspect your dada2 ASVs are numbered differently between cohorts, or that they are not identical ?
A quick check to make sure this is not caused by lotus2: Please run the same ASV file (eg from cohort A) two or three times through tax assign with lambda. This should always result in the same taxonomic assignments.