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hi, Error in RunHarmony.Seurat(object = merged_seurat, group.by.var = "orig.ident", : argument 3 matches multiple formal arguments
when I run seurat <- harmony::RunHarmony(object =seurat, group.by.vars="orig.ident", reduction = "pca", assay.use="RNA", plot_convergence = T, verbose = T)
when I removed reduction = "pca", it worked, but the harmony dimension drops to 4, no idea what happened, it seems the harmony integration worked
seurat was from a merged seurat object from two seurat object by merging a group of sample of each.
do you know what happened the error?
many thanks
The text was updated successfully, but these errors were encountered:
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hi,
Error in RunHarmony.Seurat(object = merged_seurat, group.by.var = "orig.ident", :
argument 3 matches multiple formal arguments
when I run seurat <- harmony::RunHarmony(object =seurat, group.by.vars="orig.ident",
reduction = "pca", assay.use="RNA", plot_convergence = T, verbose = T)
when I removed reduction = "pca", it worked, but the harmony dimension drops to 4, no idea what happened, it seems the harmony integration worked
seurat was from a merged seurat object from two seurat object by merging a group of sample of each.
do you know what happened the error?
many thanks
The text was updated successfully, but these errors were encountered: