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demos.md

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Demos
Video demos for NBLAST
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/demos/

{% include JB/setup %}

Overview

An introduction to NBLAST from Greg.

<iframe width="640" height="390" src="https://www.youtube.com/embed/tyg6kbixuaM" frameborder="0" allowfullscreen></iframe>

NBLAST Online

Search options

Very quick example of comparing 2 FlyCircuit neurons.

<iframe width="480" height="360" src="https://www.youtube.com/embed/FnC64OLTsp8?rel=0" frameborder="0" allowfullscreen></iframe>

Detailed example of using a neuron mentioned in a recent paper on sleep by its FlyCircuit identifier to find structurally related neurons.

<iframe width="640" height="390" src="https://www.youtube.com/embed/KmTznynrwyk" frameborder="0" allowfullscreen></iframe>

Example of searching for related neurons with a neuron id, showing the links from the results to virtualflybrain.org.

<iframe width="640" height="390" src="http://www.youtube.com/embed/jqWwQusCTJI" frameborder="0" allowfullscreen></iframe>

Searching for GMR GAL4 lines that label specific FlyCircuit neurons

<iframe width="640" height="390" src="http://www.youtube.com/embed/lJBKPAXPJB4" frameborder="0" allowfullscreen></iframe>

Detailed example of making a tracing of a part of a group of neurons in registered image data downloaded from virtualflybrain.org.

<iframe width="640" height="390" src="https://www.youtube.com/embed/xdqAb1fC91I" frameborder="0" allowfullscreen></iframe>

Traced neurons can then be used as a query for NBLAST online (detailed run through).

<iframe width="640" height="390" src="https://www.youtube.com/embed/Vn8zTEQOwmk" frameborder="0" allowfullscreen></iframe>

Detailed example of making a skeletonised image from a lineage clone, with registered image data downloaded from virtualflybrain.org and using that for an NBLAST search.

<iframe width="640" height="390" src="http://www.youtube.com/embed/pLk-GSCmg5U" frameborder="0" allowfullscreen></iframe>

Local install

<iframe width="640" height="360" src="https://www.youtube.com/embed/cCDql94lSwI?rel=0" frameborder="0" allowfullscreen></iframe>

Exploring Neuron Clusters

####Starting from a search for a brain region (the AMMC) on the Virtual Fly Brain website we show how to look at structurally related neurons in 3D in your browser.

<iframe width="480" height="360" src="https://www.youtube.com/embed/YFsxjkdr5y8?rel=0" frameborder="0" allowfullscreen></iframe>

NBLAST Desktop

Installation

<iframe width="480" height="360" src="https://www.youtube.com/embed/C9arQ0Sws7k?rel=0" frameborder="0" allowfullscreen></iframe>

Assuming you have a neuron tracing, here is how to find the most similar neuron in a database in R using the nat.nblast package.

<iframe width="480" height="360" src="https://www.youtube.com/embed/5KZIN6u2aDw?rel=0" frameborder="0" allowfullscreen></iframe>

Here is an example of how to run code that generates some of the figures in our paper. The code for all figures is available on our lab github repo.

<iframe width="480" height="360" src="https://www.youtube.com/embed/LJgZejabqqg?rel=0" frameborder="0" allowfullscreen></iframe>

Troubleshooting

We recommend Chrome for NBLAST online, but you can also use Firefox or Safari. For Safari you must activate WebGL: Here's how.

<iframe width="640" height="390" src="https://www.youtube.com/embed/u1dH9NGVFWY" frameborder="0" allowfullscreen></iframe>