diff --git a/.gitignore b/.gitignore
index e669845172..6c823299f6 100755
--- a/.gitignore
+++ b/.gitignore
@@ -1,54 +1,55 @@
 syntax: glob
-.spyderworkspace
-.coverage
-/MANIFEST
-.hook_ignore*
-*.so
-*.pyc
-*.pyd
-*.swp
 *~
 *.bak
+*.deb
 *.mex
+*nbc
+*nbi
 *.o
-dist
-*.deb
+*.pyc
+*.pyd
+*.pyproj
+*.so
 *.swo
+*.swp
+*.DS_Store
+*__pycache__
+*Thumbs.db
+/.cache/
+/.spyproject/
+.coverage
+.hook_ignore*
+.idea/*
+.python-version
+.ropeproject/*
+.spyderproject
+.spyderworkspace
+~.vimrc
+desktop.ini
+dist
+/MANIFEST
 build/*
 bin/*.bat
 PortableInstall/*
 hyperspy.egg-info/*
-deb_dist/*
-~.vimrc
 data/octave-core
-.spyderproject
+deb_dist/*
 doc/_build/*
 doc/.build/*
 doc/api/*
-doc/user_guide/_build/*
-doc/user_guide/.build/*
-setup/windows/requires/*
-setup/windows/reccomends/*
 doc/auto_examples
-examples/io/*.msa
 doc/gh-pages
 doc/log.txt
-*Thumbs.db
-*.DS_Store
-.ropeproject/*
-.idea/*
-desktop.ini
-*.pyproj
-hyperspy/tests/misc/cython/test_cython_integration.c
+doc/user_guide/_build/*
+doc/user_guide/.build/*
+examples/io/*.msa
+examples/region_of_interest/*.msa
 hyperspy/io_plugins/unbcf_fast.c
 hyperspy/misc/etc/test_compilers.obj
-/.cache/
-/.spyproject/
-*nbc
-*nbi
 hyperspy/tests/io/edax_files.zip
-.python-version
-*__pycache__
+hyperspy/tests/misc/cython/test_cython_integration.c
+setup/windows/requires/*
+setup/windows/reccomends/*
 
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