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nextflow.config
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/*
* -----------------------------------------------------
* UCL-BLIC/rnaseq_variant_calling Nextflow config file
* -----------------------------------------------------
* Default config options for all environments.
* Cluster-specific config options should be saved
* in the conf folder and imported under a profile
* name here.
*/
// Global default params, used in configs
params {
// Pipeline Options
genome = false
forward_stranded = false
reverse_stranded = false
unstranded = false
splicesites = false
outdir = './results'
saveReference = false
saveTrimmed = false
saveAlignedIntermediates = false
singleEnd = false
reads = "data/*{1,2}.fastq.gz"
// Custom trimming options
clip_r1 = 0
clip_r2 = 0
three_prime_clip_r1 = 0
three_prime_clip_r2 = 0
// Defaults
sampleLevel = false
clusterOptions = false
maxMultiqcEmailFileSize = 25.MB
readPaths = null
tracedir = "${params.outdir}"
}
profiles {
legion {
includeConfig 'conf/legion.config'
}
myriad {
includeConfig 'conf/myriad.config'
}
}
// Capture exit codes from upstream processes when piping
process.shell = ['/bin/bash', '-euo', 'pipefail']
timeline {
enabled = true
file = "${params.tracedir}/pipeline_info/rnaseq_variant_calling_timeline.html"
}
report {
enabled = true
file = "${params.tracedir}/pipeline_info/rnaseq_variant_calling_report.html"
}
trace {
enabled = true
file = "${params.tracedir}/pipeline_info/rnaseq_variant_calling_trace.txt"
}
dag {
enabled = true
file = "${params.tracedir}/pipeline_info/rnaseq_variant_calling_DAG.svg"
}
manifest {
name = 'UCL-BLIC/rnaseq_variant_calling'
description = 'Nextflow RNA-Seq variant calling pipeline.'
homePage = 'https://github.com/UCL-BLICrnaseq_variant_calling'
author = 'LC'
version = '1.1'
mainScript = 'main.nf'
nextflowVersion = '>=0.32.0'
}
// Function to ensure that resource requirements don't go beyond
// a maximum limit
def check_max(obj, type) {
if(type == 'memory'){
try {
if(obj.compareTo(params.max_memory as nextflow.util.MemoryUnit) == 1)
return params.max_memory as nextflow.util.MemoryUnit
else
return obj
} catch (all) {
println " ### ERROR ### Max memory '${params.max_memory}' is not valid! Using default value: $obj"
return obj
}
} else if(type == 'time'){
try {
if(obj.compareTo(params.max_time as nextflow.util.Duration) == 1)
return params.max_time as nextflow.util.Duration
else
return obj
} catch (all) {
println " ### ERROR ### Max time '${params.max_time}' is not valid! Using default value: $obj"
return obj
}
} else if(type == 'cpus'){
try {
return Math.min( obj, params.max_cpus as int )
} catch (all) {
println " ### ERROR ### Max cpus '${params.max_cpus}' is not valid! Using default value: $obj"
return obj
}
}
}