Question about QuickMerge use for multiple assemblies

[Edouard94]

Dear Mahulchak,

First, thank you for developing your tool and staying involved with it.

I used your tool to merge various assemblies obtained after splitting a large FASTQ file into 24 chunks and assembling each chunk individually with Megahit. I had to split my FASTQ file because my computer did not have enough resources to assemble the large file directly.

After assembling all the chunks, I created a 'hybrid_assembly.fasta' by concatenating half of my assemblies (12) and the rest as 'self_assembly.fasta'.

I then ran the command merge_wrapper.py -pre merged_output hybrid_assembly.fasta self_assembly.fasta.

Does it make sense to follow this procedure when having more than two initial assemblies? Thank you for your insights on this matter.

Best wishes,
Edouard