diff --git a/malariagen_data/anoph/pca.py b/malariagen_data/anoph/pca.py
index 6d819ba4e..a5a28adeb 100644
--- a/malariagen_data/anoph/pca.py
+++ b/malariagen_data/anoph/pca.py
@@ -62,8 +62,12 @@ def pca(
         sample_indices: Optional[base_params.sample_indices] = None,
         site_mask: Optional[base_params.site_mask] = base_params.DEFAULT,
         site_class: Optional[base_params.site_class] = None,
-        min_minor_ac: Optional[base_params.min_minor_ac] = None,
-        max_missing_an: Optional[base_params.max_missing_an] = None,
+        min_minor_ac: Optional[
+            base_params.min_minor_ac
+        ] = pca_params.min_minor_ac_default,
+        max_missing_an: Optional[
+            base_params.max_missing_an
+        ] = pca_params.max_missing_an_default,
         cohort_size: Optional[base_params.cohort_size] = None,
         min_cohort_size: Optional[base_params.min_cohort_size] = None,
         max_cohort_size: Optional[base_params.max_cohort_size] = None,
@@ -73,7 +77,7 @@ def pca(
     ) -> Tuple[pca_params.df_pca, pca_params.evr]:
         # Change this name if you ever change the behaviour of this function, to
         # invalidate any previously cached data.
-        name = "pca_v2"
+        name = "pca_v3"
 
         # Normalize params for consistent hash value.
         (
diff --git a/malariagen_data/anoph/pca_params.py b/malariagen_data/anoph/pca_params.py
index e74a243af..ef959509d 100644
--- a/malariagen_data/anoph/pca_params.py
+++ b/malariagen_data/anoph/pca_params.py
@@ -3,6 +3,7 @@
 import numpy as np
 import pandas as pd
 from typing_extensions import Annotated, TypeAlias
+from . import base_params
 
 n_components: TypeAlias = Annotated[
     int,
@@ -23,3 +24,7 @@
     np.ndarray,
     "An array of explained variance ratios, one per component.",
 ]
+
+min_minor_ac_default: base_params.min_minor_ac = 2
+
+max_missing_an_default: base_params.max_missing_an = 0
diff --git a/malariagen_data/anoph/snp_data.py b/malariagen_data/anoph/snp_data.py
index 8e16c3552..7b404f3a8 100644
--- a/malariagen_data/anoph/snp_data.py
+++ b/malariagen_data/anoph/snp_data.py
@@ -1655,7 +1655,7 @@ def biallelic_snp_calls(
             ds_out = xr.Dataset(coords=coords, data_vars=data_vars, attrs=ds.attrs)
 
             # Apply conditions.
-            if max_missing_an or min_minor_ac:
+            if max_missing_an is not None or min_minor_ac is not None:
                 loc_out = np.ones(ds_out.sizes["variants"], dtype=bool)
 
                 # Apply missingness condition.
diff --git a/malariagen_data/anopheles.py b/malariagen_data/anopheles.py
index 615215858..324cc395e 100644
--- a/malariagen_data/anopheles.py
+++ b/malariagen_data/anopheles.py
@@ -35,6 +35,7 @@
     hapnet_params,
     het_params,
     ihs_params,
+    pca_params,
     plotly_params,
     xpehh_params,
 )
@@ -3158,8 +3159,12 @@ def plot_njt(
         sample_indices: Optional[base_params.sample_indices] = None,
         site_mask: Optional[base_params.site_mask] = base_params.DEFAULT,
         site_class: Optional[base_params.site_class] = None,
-        min_minor_ac: Optional[base_params.min_minor_ac] = None,
-        max_missing_an: Optional[base_params.max_missing_an] = None,
+        min_minor_ac: Optional[
+            base_params.min_minor_ac
+        ] = pca_params.min_minor_ac_default,
+        max_missing_an: Optional[
+            base_params.max_missing_an
+        ] = pca_params.max_missing_an_default,
         cohort_size: Optional[base_params.cohort_size] = None,
         min_cohort_size: Optional[base_params.min_cohort_size] = None,
         max_cohort_size: Optional[base_params.max_cohort_size] = None,
diff --git a/tests/anoph/test_pca.py b/tests/anoph/test_pca.py
index 554644f98..8d452fcce 100644
--- a/tests/anoph/test_pca.py
+++ b/tests/anoph/test_pca.py
@@ -9,6 +9,7 @@
 from malariagen_data import af1 as _af1
 from malariagen_data import ag3 as _ag3
 from malariagen_data.anoph.pca import AnophelesPca
+from malariagen_data.anoph import pca_params
 
 
 @pytest.fixture
@@ -83,13 +84,18 @@ def test_pca_plotting(fixture, api: AnophelesPca):
         sample_sets=random.sample(all_sample_sets, 2),
         site_mask=random.choice((None,) + api.site_mask_ids),
     )
-    ds = api.biallelic_snp_calls(**data_params)
+    ds = api.biallelic_snp_calls(
+        min_minor_ac=pca_params.min_minor_ac_default,
+        max_missing_an=pca_params.max_missing_an_default,
+        **data_params,
+    )
 
     # PCA parameters.
     n_samples = ds.sizes["samples"]
     n_snps_available = ds.sizes["variants"]
-    n_snps = random.randint(n_samples, n_snps_available)
-    n_components = random.randint(3, n_samples)
+    n_snps = random.randint(1, n_snps_available)
+    # PC3 required for plot_pca_coords_3d()
+    n_components = random.randint(3, min(n_samples, n_snps))
 
     # Run the PCA.
     pca_df, pca_evr = api.pca(