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Cutadapt is used quite often to trim primer sequences, in which case it is expected that the "adapter" (primer) is not preceded/followed by a random nucleotide, but a specific one.
Cutadapt is used quite often to trim primer sequences, in which case it is expected that the "adapter" (primer) is not preceded/followed by a random nucleotide, but a specific one.
The warning was added to prevent people from providing an Illumina adapter that is missing the initial
A
. Instead, we could directly check for the Illumina adapter sequence (and only that one).cf #779
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