Error during TrimGalore step

[timyerg]

Hi!
Thank you for the tool.
I am trying to run Module1 with my data on the server. QC step started normally and I managed to obtain "sample_1_val_1.fq.gz file and the same for 2 read.

Then, qc stats gives me an error:

RUN STATISTICS FOR INPUT FILE: /beegfs/work/graaf20/PFOWL2-3/Clean_reads/UHO333_2.fq.gz
=============================================
36566934 sequences processed in total
The length threshold of paired-end sequences gets evaluated later on (in the validation step)

Validate paired-end files UHO333_1_trimmed.fq.gz and UHO333_2_trimmed.fq.gz
file_1: UHO333_1_trimmed.fq.gz, file_2: UHO333_2_trimmed.fq.gz


>>>>> Now validing the length of the 2 paired-end infiles: UHO333_1_trimmed.fq.gz and UHO333_2_trimmed.fq.gz <<<<<
Writing validated paired-end Read 1 reads to UHO333_1_val_1.fq.gz
Writing validated paired-end Read 2 reads to UHO333_2_val_2.fq.gz

Total number of sequences analysed: 36566934

Number of sequence pairs removed because at least one read was shorter than the length cutoff (20 bp): 25474 (0.07%)

Deleting both intermediate output files UHO333_1_trimmed.fq.gz and UHO333_2_trimmed.fq.gz

====================================================================================================

mv: cannot stat '/tools/mudoger_output_in_container/UHO333/qc/UHO333_1_val_1.fq': No such file or directory
mv: cannot stat '/tools/mudoger_output_in_container/UHO333/qc/UHO333_2_val_2.fq': No such file or directory

************************************************************************************************************************
*****                            Something went wrong with trimming the reads. Exiting.                            *****
************************************************************************************************************************

Is it because my file gziped? Should they be ungziped?

Since my samples already quality controlled and clean from host contamination, I will perform assembly outside and proceed to module2. Just reporting here in case if the problem was with gziped files. Is there an option to use it instead of ungziped? I have a large dataset to handle.