question about merged miRNAs

[hust-yyan]

Hi, I found merged miRNA in the annotation file, but they are in different locations of the genome, according to what criteria is merge done?If I want to count separately, can I assign expression values to each miRNA?Now I am doing eQTL research for miRNA, which requires the precise location of each miRNA in the genome, but merged miRNA makes me not know how to deal with it.

[mhalushka]

Hi. miRge3.0 does not distinguish between genomic locations when a miRNA has more than one site, such as let-7a. I know other programs do this, but I think their assignments are arbitrary. There is no information in a FASTQ that can help determine location when the sequence exactly matches more than one genomic sequence.

[arunhpatil]

Hi @hust-yyan, you can find the link to miRge3.0 here.