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How can I get files like tagMir-all.gff? #57

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illumination-k opened this issue Sep 5, 2019 · 1 comment
Open

How can I get files like tagMir-all.gff? #57

illumination-k opened this issue Sep 5, 2019 · 1 comment

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@illumination-k
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illumination-k commented Sep 5, 2019

Hi,
I would like to analyze isomiRs by using isomiR-SEA ,mirtop and R-package(isomiRs).

Your quick start example to import isomiR-SEA files is
(and maybe your example of 'isomiR-SEA' is example of 'validation')


mirtop gff --format isomirsea -sps hsa --hairpin  examples/annotate/hairpin.fa --gtf  examples/annotate/hsa.gff3 -o  test_out examples/isomir-sea/tagMir-all.gff

But I think isomiR-SEA generate just below files...
• out_result_mature_21_ambigue.txt
• out_result_mature_21_ambigue_ambigue_selected.txt
• out_result_mature_21_tag_ambigue.txt
• out_result_mature_21_tag_ambigue_selected.txt
• out_result_mature_21_tag_unique.txt
• out_result_mature_21_unique.txt
• out_result_mature_21_unique_ambigue_selected.txt

So I wondered how can I get files like tagMir-all.gff.

Can you tell me how can I generate this?

@lpantano
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lpantano commented Sep 5, 2019

Thank very much for the question, I will tag @gurgese who is the developer of isomir-sea. I will add that to the documentation when we get the answer from him.

Thanks!

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