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Repeated isomiRs in the mirtop.tsv output #80

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bounlu opened this issue Apr 30, 2024 · 0 comments
Open

Repeated isomiRs in the mirtop.tsv output #80

bounlu opened this issue Apr 30, 2024 · 0 comments

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@bounlu
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bounlu commented Apr 30, 2024

The mirtop.tsv output contains repeated rows for some isomiRs with identical first 12 columns:

UID  Read  miRNA  Variant  iso_5p  iso_3p  iso_add3p  iso_snp  iso_5p_nt  iso_3p_nt  iso_add3p_nt  iso_snp_nt
iso-16-03170FE  ACAGTAGTCTACACAT  hsa-miR-199b-3p  iso_3p:-6,iso_snv_central  0  -6  0  1  0  tggtta  0  11AG
iso-16-03170FE  ACAGTAGTCTACACAT  hsa-miR-199a-3p  iso_3p:-6,iso_snv_central  0  -6  0  1  0  tggtta  0  11AG
iso-16-03170FE  ACAGTAGTCTACACAT  hsa-miR-199a-3p  iso_3p:-6,iso_snv_central  0  -6  0  1  0  tggtta  0  11AG

Their counts in the samples differ slightly though. I would expect these isomiRs to be merged and counted together in the output. I understand from mirtop.gff that they come from different precursors with identical mature sequences, but then I don't understand why their counts are different in the same sample?

Is this a bug? If not, what is the reason behind repeated isomiRs with identical sequences but different counts in the same sample?

@bounlu bounlu changed the title Repeated iromiRs in the mirtop.tsv output Repeated isomiRs in the mirtop.tsv output May 2, 2024
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