diff --git a/checkValidationNGS_DNA.sh b/checkValidationNGS_DNA.sh
index c324d4d..e138102 100755
--- a/checkValidationNGS_DNA.sh
+++ b/checkValidationNGS_DNA.sh
@@ -23,96 +23,122 @@ EOH
 	exit 0
 }
 
+function doVariantEval(){
+
+	folder="${validationFolderTmp}"
+	output="${outputFolder}"
+
+	mkdir -p "${output}"
+
+	for i in $(ls "${folder}/"*".${inputType}")
+	do
+		name=$(basename $i ".${inputType}")
+		java -jar ${EBROOTGATK}/GenomeAnalysisTK.jar \
+		-T VariantEval \
+		-R /apps/data/1000G/phase1/human_g1k_v37_phiX.fasta \
+		-o "${output}/output.${name}.eval.grp" \
+		--eval "${inputFolder}/"*"${name}"*".${inputType}" \
+		--comp "${i}"
+
+	done
+
+	for i in $(ls "${folder}/"*".${inputType}")
+	do
+
+		name=$(basename "${i}" ".${inputType}")
+		check=$(awk '{if (NR==5){if ($11 == "100.00"){print "correct"}}}' "${output}/output.${name}.eval.grp")
+		if [ "${check}" == "correct" ]
+		then
+			zcat "${i}" | awk -v sample="${name}" 'BEGIN {OFS="  "}{if ($1 !~ /^#/){print sample,$1,$2,$4,$5,"FOUND BACK"}}'
+		else
+			zcat "${i}" | awk -v sample="${name}" 'BEGIN {OFS="  "}{if ($1 !~ /^#/){print sample,$1,$2,$4,$5,"Not 100% concordant!"}}' 
+			exit 1
+		fi
+	done
+
+}
+
+function doComparisonFiltered (){
+	refCall="${1}"
+	if [ "${refCall}" == "referenceCall" ]
+        then
+		folder="${validationFolderTmp}/filtered/referenceCall"
+                output="${outputFolder}/filtered/referenceCall"
+        else
+		folder="${validationFolderTmp}/filtered/"
+                output="${outputFolder}/filtered/"
+        fi
+	mkdir -p "${output}"
+
+for i in $(ls "${folder}/"*".${inputType}")
+do
+
+	name=$(basename "${i}" ".${inputType}")
+
+        if [[ "${inputType}" == "vcf.gz" ]]
+        then
+		validationSample=$(zcat ${i} | grep -v '^#' | awk '{print $1"-"$2"-"$4"-"$5"-"$7}')
+                inputSample=$(zcat "${inputFolder}/"*"${name}"*".${inputType}" | grep 18598089 | awk '{print $1"-"$2"-"$4"-"$5"-"$7}')
+
+                if [ "${validationSample}" == "${inputSample}" ]
+                then
+			if [ "${refCall}" == "referenceCall" ]
+                        then
+				zcat "${i}" | awk -v sample="${name}" 'BEGIN {OFS="  "}{if ($1 !~ /^#/){print sample,$1,$2,$4,$5,$7,"FOUND BACK,REF CALL"}}'
+                        else
+                                zcat "${i}" | awk -v sample="${name}" 'BEGIN {OFS="  "}{if ($1 !~ /^#/){print sample,$1,$2,$4,$5,$7,"FOUND BACK"}}'
+                        fi
+                else
+			zcat "${i}" | awk -v sample="${name}" 'BEGIN {OFS="  "}{if ($1 !~ /^#/){print sample,$1,$2,$4,$5,$7,"Not 100% concordant!"}}' 
+                        zcat "${inputFolder}/"*"${name}"*".${inputType}" | grep 18598089
+                        zcat "${i}"
+                        exit 1
+                fi
+        fi
+done
+
+}
+
 
 while getopts "i:o:v:t:h" opt; 
 do
-	case $opt in h)showHelp;; i)inputFolder="${OPTARG}";; o)outputFolder="${OPTARG}";; v)validationFolder="${OPTARG}";; t)inputType="${OPTARG}";;
+	case $opt in h)showHelp;; i)inputFolder="${OPTARG}";; o)outputFolder="${OPTARG}";; v)validationFolderPrm="${OPTARG}";; t)inputType="${OPTARG}";;
 esac 
 done
 
 if [[ -z "${inputFolder:-}" ]]; then showHelp ; echo "inputFolder is not specified" ; fi ; echo "inputFolder=${inputFolder}"
 if [[ -z "${outputFolder:-}" ]]; then mkdir -p "${inputFolder}/output/" ; outputFolder="${inputFolder}/output/" ; fi ; echo "outputFolder=${outputFolder}"
 if [[ -z "${inputType:-}" ]]; then inputType="vcf.gz" ; fi ; echo "inputType=${inputType}"
-if [[ -z "${validationFolder:-}" ]]; then validationFolder="/groups/umcg-gd/prm03/projects/validationVcfs/" ; fi ; echo "validationFolder=${validationFolder}"
+if [[ -z "${validationFolderPrm:-}" ]]; then validationFolderPrm="/groups/umcg-gd/prm03/projects/validationVcfs/" ; fi ; echo "validationFolderPrm=${validationFolderPrm}"
 
 ml GATK 
 
 
 if [ $(hostname) != "calculon" ]
 then
-	mkdir -p "${inputFolder}/validationVcfs/filtered"
+	validationFolderTmp=${inputFolder}/validationVcfs/
+	mkdir -p "${outputFolder}/filtered/"
+
 	echo "copying validationVcfs"
-	if [ -f "${inputFolder}/validationVcfs/DNA087244.${inputType}" ]
+	if [ -f "${validationFolderTmp}/DNA087244.${inputType}" ]
 	then
 		echo "already copied, skipped"
 	else
-		scp calculon.hpc.rug.nl:${validationFolder}/*{.gz,tbi} "${inputFolder}/validationVcfs/"
-		scp calculon.hpc.rug.nl:${validationFolder}/filtered/* "${inputFolder}/validationVcfs/filtered/"
+		scp -r calculon.hpc.rug.nl:${validationFolderPrm}/ "${validationFolderTmp}/"
+		#scp calculon.hpc.rug.nl:${validationFolderPrm}/referenceCall/*{.gz,tbi} "${validationFolderTmp}/referenceCall/"
+		#scp -r calculon.hpc.rug.nl:${validationFolderPrm}/filtered/* "${validationFolderTmp}/filtered/"
 	fi
-	validationFolder="${inputFolder}/validationVcfs/"
-fi
-for i in $(ls "${validationFolder}/"*".${inputType}")
-do
-	name=$(basename $i ".${inputType}")
-
-	java -jar ${EBROOTGATK}/GenomeAnalysisTK.jar \
-	-T VariantEval \
-	-R /apps/data/1000G/phase1/human_g1k_v37_phiX.fasta \
-	-o "${outputFolder}/output.${name}.eval.grp" \
-	--eval "${inputFolder}/"*"${name}"*".${inputType}" \
-	--comp "${i}"
-
-done
-
-for i in $(ls "${validationFolder}/"*".${inputType}")
-do
-        name=$(basename "${i}" ".${inputType}")
-
-        referenceCall=$(grep "0/0" "${i}" | wc -l)
-        referenceCallMale=$(grep -P "\t0:" "${i}" | wc -l)
-
-        check=$(awk '{if (NR==5){if ($11 == "100.00"){print "correct"}}}' "${outputFolder}/output.${name}.eval.grp")
-	if [[ "${inputType}" == "vcf.gz" ]]
-	then
-		if [ "${check}" == "correct" ]
-		then
-			zcat "${i}" | awk -v sample="${name}" 'BEGIN {OFS="  "}{if ($1 !~ /^#/){print sample,$1,$2,$4,$5,"FOUND BACK"}}'
-		elif [[ "${referenceCall}" -ne 0 || "${referenceCallMale}" -ne 0 ]]
-		then
-			zcat "${i}" | awk -v sample="${name}" 'BEGIN {OFS="  "}{if ($1 !~ /^#/){print sample,$1,$2,$4,$5,"FOUND BACK,REF CALL"}}'
-		else
-			zcat "${i}" | awk -v sample="${name}" 'BEGIN {OFS="  "}{if ($1 !~ /^#/){print sample,$1,$2,$4,$5,"Not 100% concordant!"}}' 
-			exit 1
-		fi
-	elif [[ "${inputType}" == "vcf" ]]
-	then
-		if [ "${check}" == "correct" ]
-		then
-			awk -v sample="${name}" 'BEGIN {OFS="  "}{if ($1 !~ /^#/){print sample,$1,$2,$4,$5,"FOUND BACK"}}' "${i}"
-		elif [[ "${referenceCall}" -ne 0 || "${referenceCallMale}" -ne 0 ]]
-		then
-			awk -v sample="${name}" 'BEGIN {OFS="  "}{if ($1 !~ /^#/){print sample,$1,$2,$4,$5,"FOUND BACK,REF CALL"}}' "${i}"
-		else
-			awk -v sample="${name}" 'BEGIN {OFS="  "}{if ($1 !~ /^#/){print sample,$1,$2,$4,$5,"Not 100% concordant!"}}' "${i}"
-			exit 1
-		fi
+else
+	validationFolderTmp=${inputFolder}/validationVcfs/
+	if [ -f "${validationFolderTmp}/DNA087244.${inputType}" ]
+        then
+		echo "already copied, skipped"
+        else
+		cp -r calculon.hpc.rug.nl:${validationFolderPrm}/ "${validationFolderTmp}/"
 	fi
-done
-
-for i in $(ls "${validationFolder}/filtered/"*".${inputType}")
-do
-	name=$(basename "${i}" ".${inputType}")
+fi
 
-	validationSample=$(zcat ${i} | grep -v '^#' | awk '{print $1"-"$2"-"$4"-"$5"-"$7}')
-	inputSample=$(zcat "${inputFolder}/"*"${name}"*".${inputType}" | grep 18598089 | awk '{print $1"-"$2"-"$4"-"$5"-"$7}')
+doVariantEval
 
-	if [ "${validationSample}" == "${inputSample}" ]
-	then
-		zcat "${i}" | awk -v sample="${name}" 'BEGIN {OFS="  "}{if ($1 !~ /^#/){print sample,$1,$2,$4,$5,$7,"FOUND BACK"}}'
-	else
-		zcat "${i}" | awk -v sample="${name}" 'BEGIN {OFS="  "}{if ($1 !~ /^#/){print sample,$1,$2,$4,$5,$7,"Not 100% concordant!"}}' 
-		zcat "${inputFolder}/"*"${name}"*".${inputType}" | grep 18598089
-		zcat "${i}"
-		exit 1
-	fi
-done
+doComparisonFiltered "no"
+doComparisonFiltered "referenceCall"